FlyBase Gene Report: Dmel\eEF1α1

General Information

Symbol

Dmel\eEF1α1

Species

D. melanogaster

Name

eukaryotic translation elongation factor 1 alpha 1

Annotation Symbol

CG8280

Feature Type

protein_coding_gene

FlyBase ID

FBgn0284245

Gene Model Status

Current

Stock Availability

20 publicly available

Gene Summary

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. (UniProt, P08736)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

Ef1alpha48D, EF-1α, Ef1α48D, EF1α, l(2)00248

Key Links

Genomic Location

Cytogenetic map

48C5-48C5

Sequence location

2R:11,891,135..11,895,762 [+]

Recombination map

2-65

RefSeq locus

NT_033778 REGION:11891135..11895762

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (7 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables GTP binding

inferred from electronic annotation with InterPro:IPR000795, InterPro:IPR004161, InterPro:IPR004539

(InterPro Project Members, 2004-)

enables GTPase activity

inferred from electronic annotation with InterPro:IPR000795

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000561878

(GO Reference Genome Project, 2011-)

enables translation elongation factor activity

inferred from electronic annotation with InterPro:IPR004539

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000562490

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity

(Lasko, 2000)

Biological Process (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in determination of adult lifespan

inferred from mutant phenotype

(Shepherd et al., 1989)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in translational elongation

inferred from biological aspect of ancestor with PANTHER:PTN000562490

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR004539

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity

(Lasko, 2000)

involved_in translation

inferred from biological aspect of ancestor with PANTHER:PTN000561878

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of eukaryotic translation elongation factor 1 complex

inferred from sequence or structural similarity

(Lasko, 2000)

Gene Group (FlyBase)

CYTOPLASMIC TRANSLATION ELONGATION FACTORS

TRANSLATIONAL GTPASES

Protein Family (UniProt)

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily. (P08736)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

GTPase activity
RHEA 19669:

Summaries

Gene Group (FlyBase)

CYTOPLASMIC TRANSLATION ELONGATION FACTORS -: Cytoplasmic (or eukaryotic) translation elongation factors function in the chain elongation stage of ribosome-mediated polypeptide synthesis in the cytoplasm. (Adapted from FBrf0233552).
TRANSLATIONAL GTPASES -: Translational GTPases are a family of GTPases that facilitate and control protein synthesis on the ribosome. (Adapted from PMID:26971860).

Protein Function (UniProtKB)

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

(UniProt, P08736)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

Ef1α1: Elongation factor 1α #1

Encodes an α unit of the heterotrimeric protein, elongation factor 1, which promotes the binding of aminoacyl-tRNA to ribosomes. Originally isolated on the basis of its displaying 5 to 10 fold overexpression in females versus males. Ef1α1 is expressed throughout development. The rate of protein synthesis in Drosophila declines with age; this has been attributed to impaired binding of aminoacyl-tRNA to ribosomes, which is promoted by elongation- factor 1 (EF1); transcription of elongation factor-1 mRNA shown to decrease with age; by fifteen days following emergence the level of EF1 mRNA is but a few percent that of newly emerged adults (Webster and Webster, 1984, Mech. Ageing Dev. 24: 335-42). Transformation with Ef1α1 under the control of heat shock promoter increased the mean life span from 38.2 to 45.1 days at 25 and from 21.1 to 29.8 days at 29 (Shepherd et al.).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\eEF1α1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P08736)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.45

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.55

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

eEF1α1-RA

FBtr0088035

NM_058027

2029

463

eEF1α1-RC

FBtr0331927

NM_165850

2278

463

eEF1α1-RD

FBtr0345022

NM_001299392

1979

463

eEF1α1-RE

FBtr0345023

NM_001299393

2076

463

Additional Transcript Data and Comments

Reported size (kB)

2.0 (unknown)

(Walldorf et al., 1985)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

eEF1α1-PA

FBpp0087142

50.3

463

9.60

FBpp0304260

50.3

463

9.60

FBpp0311272

50.3

463

9.60

FBpp0311273

50.3

463

9.60

P08736

NP_001286322

AHN56120

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

463 aa isoforms: eEF1α1-PA, eEF1α1-PC, eEF1α1-PD, eEF1α1-PE

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\eEF1α1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.70

Transcript Expression

dot blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Walldorf et al., 1985)

Additional Descriptive Data

Ef1α48D transcript is expressed in all developmental stages. Expression in females is 5 to 10-fold higher than in males.

(Walldorf et al., 1985)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

embryonic/larval hemolymph

Comment: component of hemolymph clot

(Scherfer et al., 2004)

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood | male -- day 7 of adulthood | male

spermatozoon

(Wasbrough et al., 2010)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Isolated from a pullout assay for proteins contained in clots from larval hemolymph and identified by mass spec.

(Scherfer et al., 2004)

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

Reporter: P{PZ}eEF1α1⁰¹²⁷⁵

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

prothoracic gland

(Harvie et al., 1998)

wandering third instar larval stage

eye disc

(Harvie et al., 1998)

embryonic/larval fat body

(Meister and Braun, 1995.10)

Malpighian tubule

(Meister and Braun, 1995.10)

larval brain

(Meister and Braun, 1995.10)

imaginal disc

(Meister and Braun, 1995.10)

embryonic/larval optic lobe

(Harvie et al., 1998)

larval ventral nerve cord

(Harvie et al., 1998)

adult stage

adult central complex

(Vosshall, 1998.8.10)

adult brain | restricted

(Vosshall, 1998.8.10)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\eEF1α1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\eEF1α1

View exonic expression by tissue for Dmel\eEF1α1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36271/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0284245

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0000556

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0284245

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8280

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 17 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 12 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of eEF1α1

Transgenic constructs containing regulatory region of eEF1α1

Aberrations (Deficiencies and Duplications) ( 32 )

Inferred from experimentation ( 32 )

Gene disrupted in

Df(2R)BSC25

(Marygold et al., 2007)

Df(2R)BSC153

(Marygold et al., 2007)

Df(2R)BSC329

(Kahsai et al., 2016)

Df(2R)BSC879

(Kahsai et al., 2016)

Gene partially disrupted in

Df(2R)BSC25

(Stathopoulos et al., 2004)

Gene not disrupted in

Df(2R)50C-16

(Preston et al., 1996)

Df(2R)50C-21

(Preston et al., 1996)

Df(2R)50C-36

(Preston et al., 1996)

Df(2R)50C-38

(Preston et al., 1996, BDGP Project Members, 1994-1999)

Df(2R)50C-40

(Preston et al., 1996)

Df(2R)50C-43

(Preston et al., 1996)

Df(2R)50C-45

(Preston et al., 1996)

Df(2R)50C-49

(Preston et al., 1996)

Df(2R)50C-68

(Preston et al., 1996)

Df(2R)50C-69

(Preston et al., 1996)

Df(2R)50C-71

(Preston et al., 1996)

Df(2R)50C-81

(Preston et al., 1996)

Df(2R)50C-85

(Preston et al., 1996)

Df(2R)50C-89

(Preston et al., 1996)

Df(2R)50C-101

(Preston et al., 1996, BDGP Project Members, 1994-1999)

Df(2R)50C-102

(Preston et al., 1996)

Df(2R)50C-107

(Preston et al., 1996)

Df(2R)50C-111

(Preston et al., 1996)

Df(2R)50C-116

(Preston et al., 1996)

Df(2R)50C-128

(Preston et al., 1996)

Df(2R)50C-129

(Preston et al., 1996)

Df(2R)50C-131

(Preston et al., 1996)

Df(2R)50C-132

(Preston et al., 1996)

Df(2R)50C-133

(Preston et al., 1996)

Df(2R)CX1

(BDGP Project Members, 1994-1999)

Df(2R)en30

(BDGP Project Members, 1994-1999)

Df(2R)vg135

(BDGP Project Members, 1994-1999)

In(2R)vg^W

(BDGP Project Members, 1994-1999)

Inferred from location ( 9 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Act5C.PU

lethal, with Scer\GAL4^Bx-MS1096

lethal, with Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH

lethal, with Scer\GAL4^pnr-MD237

lethal, with Scer\GAL4^tin.CΔ4

eEF1α1^GD13875

lethal (with Df(2R)BSC329)

eEF1α1⁰⁰²⁴⁸

lethal (with Df(2R)BSC879)

eEF1α1⁰⁰²⁴⁸

lethal (with eEF1α1⁰⁰²⁴⁸)

eEF1α1⁰¹²⁷⁵

lethal (with eEF1α1⁰¹²⁷⁵)

eEF1α1⁰⁰²⁴⁸

lethal | pupal stage, with Scer\GAL4^pnr-MD237

eEF1α1^GD13875

lethal | recessive

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

eEF1α1^GD17848

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

eEF1α1^GD13875

Sterility

Allele

female sterile, with Scer\GAL4^nanos.PG

Other Phenotypes

Allele

decreased speed of aging

eEF1α1^hs.PS

some die during pupal stage, with Scer\GAL4^pnr-MD237

eEF1α1^GD17848

Phenotype manifest in

Allele

sarcomere, with Scer\GAL4^Mef2.PR

eEF1α1^GD13875

Z disc, with Scer\GAL4^Mef2.PR

eEF1α1^GD13875

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (17)

Hsap\EEF1A2

12 of 14

Yes

Hsap\EEF1A1

11 of 14

Yes

Hsap\EEF1A1P5

9 of 14

Yes

4 of 14

4 of 14

4 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (9)

Rnor\Eef1a1

12 of 14

Yes

Rnor\Eef1a2

12 of 14

Yes

Rnor\LOC100360413

11 of 14

Yes

4 of 14

4 of 14

4 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (8)

Mmus\Eef1a1

12 of 14

Yes

11 of 14

4 of 14

4 of 14

4 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (11)

Xtro\eef1a1

11 of 13

Yes

Xtro\eef1a2

10 of 13

Xtro\42Sp50

6 of 13

Yes

Xtro\adam28.1

6 of 13

Xtro\eef1a1o

6 of 13

Yes

3 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (16)

Drer\eef1a1l1

13 of 14

Yes

Drer\eef1a1a

12 of 14

Yes

Drer\eef1a2

12 of 14

Drer\si:dkey-37o8.1

8 of 14

Yes

4 of 14

4 of 14

3 of 14

2 of 14

Yes

Drer\eef1a1b

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (13)

Cele\eef-1A.1

14 of 14

Yes

Cele\eef-1A.2

13 of 14

Yes

3 of 14

3 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (11)

Agam\AgaP_AGAP007406

12 of 12

Yes

5 of 12

4 of 12

4 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (18)

Atha\AT1G07920

12 of 13

Yes

Atha\AT1G07930

12 of 13

Yes

Atha\AT1G07940

12 of 13

Yes

Atha\AT5G60390

12 of 13

Yes

Atha\AT1G18070

4 of 13

Atha\AT5G10630

2 of 13

Yes

Atha\AT1G35550

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (10)

Scer\TEF1

13 of 13

Yes

Scer\TEF2

13 of 13

Yes

Scer\HBS1

5 of 13

Scer\SKI7

3 of 13

Yes

3 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (7)

Spom\ef1a-a

12 of 12

Yes

Spom\ef1a-c

12 of 12

Yes

Spom\tef102

12 of 12

Yes

Spom\SPBC25B2.01

4 of 12

Spom\sup35

3 of 12

Spom\SPAP8A3.05

1 of 12

Yes

Spom\tuf1

1 of 12

Escherichia coli (enterobacterium) (4)

Ecol\cysN

8 of 11

Yes

Ecol\selB

1 of 11

Ecol\tufA

1 of 11

Ecol\tufB

1 of 11

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:eEF1α1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (17)

11 of 13

9 of 13

9 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

waw

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 2 )

Human Ortholog

Disease

Evidence

References

EEF1A2; eukaryotic translation elongation factor 1 alpha 2

model of autosomal dominant intellectual developmental disorder 38

IEA

(FlyBase, 2019-)

model of developmental and epileptic encephalopathy 33

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

eEF1α1^RNAi.UAS.cUa

ameliorates X-linked panhypopituitarism

modeled by SoxN^A34.UAS

(Li et al., 2017)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

EEF1A2; eukaryotic translation elongation factor 1 alpha 2

12 of 14

602959

EEF1A1; eukaryotic translation elongation factor 1 alpha 1

11 of 14

130590

EEF1A1P5; eukaryotic translation elongation factor 1 alpha 1 pseudogene 5

9 of 14

GSPT1; G1 to S phase transition 1

4 of 14

139259

GSPT2; G1 to S phase transition 2

4 of 14

300418

HBS1L; HBS1 like translational GTPase

4 of 14

612450

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

eEF1α1 - Akt

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eEF1α1 - CG11807

experimental knowledge based

(Guruharsha et al., 2011)

eEF1α1 - S6k

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eEF1α1 - ZIPIC

anti bait coimmunoprecipitation, anti tag western blot, pull down, western blot

(Zolotarev et al., 2016)

eEF1α1 - dwg

pull down, Identification by mass spectrometry, western blot, anti bait coimmunoprecipitation, anti tag western blot

(Zolotarev et al., 2016)

eEF1α1 - grau

pull down, Identification by mass spectrometry, western blot, anti bait coimmunoprecipitation, anti tag western blot

(Zolotarev et al., 2016)

eEF1α1 - h-cup

experimental knowledge based

(Guruharsha et al., 2011)

eEF1α1 - trus

tandem affinity purification, Identification by mass spectrometry

(Takada et al., 2025)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Mettl1

suppressible

eEF1α1

(Kaneko et al., 2024)

suppressible

eEF1α1

(McClure and Schubiger, 2008)

External Data

Linkouts

MIST (protein-protein) - An integrated Molecular Interaction Database

36271

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Nucleocytoplasmic transport

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

(dos Santos, 2014, Bai et al., 2007, Langille and Clark, 2007, Betran et al., 2002)

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-65

Cytogenetic map

48C5-48C5

Sequence location

2R:11,891,135..11,895,762 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

48C5-48C5

Limits computationally determined from genome sequence between P{EP}wal^EP825&P{PZ}wal⁰²⁵¹⁶ and P{PZ}Ef1α48D⁰¹²⁷⁵

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

48C5-48C6

(determined by in situ hybridisation)

(Spradling et al., 1999)

50D5-50D7

(determined by in situ hybridisation)

(Preston et al., 1996)

48C1-50D7

48C5--6 48C6--8 48C7--8 48D1--2

(BDGP Project Members, 1994-1999)

48D-48D

(determined by in situ hybridisation)

(Walldorf et al., 1985)

Experimentally Determined Recombination Data

Location

2-65

(FlyBase, 2016)

2-66.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

(Preston and Engels, 1996)

Notes

Determined by deletion mapping.

(Preston and Engels, 1996)

Stocks and Reagents

Stocks (20)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

10935

cn¹ P{PZ}eEF1α1⁰⁰²⁴⁸/CyO

28393

y¹ w^*; P{EP}eEF1α1^G13120

11034

cn¹ P{PZ}eEF1α1⁰¹²⁷⁵/CyO; ry⁵⁰⁶

10397

y¹ w^67c23; P{lacW}eEF1α1^k06102/CyO

19959

y¹ w^67c23; P{EPgy2}eEF1α1^EY09615/CyO

Kyoto

102440

y¹ w^67c23 ; P{lacW}eEF1α1^k06102 / CyO

102552

y¹ w^67c23 ; P{lacW}eEF1α1^k07303 / CyO

102567

y¹ w^67c23 ; P{lacW}eEF1α1^k07507 / CyO

VDRC

v104502

P{KK109406}VIE-260B

v24270

w¹¹¹⁸; P{GD13875}v24270

v24271

w¹¹¹⁸; P{GD13875}v24271

v49890

w¹¹¹⁸; P{GD17848}v49890/TM3

More stocks available...

Genomic Clones (10)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (1986)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

RH01053

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland.

(Harvie et al., 1998)

In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.

(Kliman and Eyre-Walker, 1998)

Expression of transcript changes with adult age.

(Shikama and Brack, 1996)

Expression of Ef1α100E or Ef1α48D is not associated with an extended longevity phenotype.

(Dudas and Arking, 1994)

The report (FBrf0050863) that expression of Ef1α48D correlates with longevity cannot be confirmed by a study of the expression of this gene and of Ef1α100E in lines with extended longevity.

(Dudas and Arking, 1994)

An important aging control may be exerted at the level of Ef1α48D transcription but heat induced expression of Ef1α48D does not cause increased levels of Ef1α48D mRNA or protein.

(Shikama et al., 1994)

Enhanced expression of Ef1α48D increases the lifespan of mated females and makes local changes within the bounds of tradeoffs that are given by a pre-existing physiological structure whose basic nature is not changed by the treatment.

(Stearns and Kaiser, 1993)

Ef1α48D is the housekeeping gene that is needed in all growing cells. Individuals transformed with a P-element containing Ef1α48D sequences under heat shock control have a longer lifespan than wild type flies.

(Shepherd et al., 1989)

Encodes an α unit of the heterotrimeric protein, elongation factor 1, which promotes the binding of aminoacyl-tRNA to ribosomes. Originally isolated on the basis of its displaying 5 to 10 fold overexpression in females versus males. Ef1α1 is expressed throughout development. The rate of protein synthesis in Drosophila declines with age; this has been attributed to impaired binding of aminoacyl-tRNA to ribosomes, which is promoted by elongation-factor 1 (EF1); transcription of elongation factor-1 mRNA shown to decrease with age; by fifteen days following emergence the level of EF1 mRNA is but a few percent that of newly emerged adults (Webster and Webster, 1984). Transformation with Ef1α1 under the control of heat shock promoter increased the mean life span from 38.2 to 45.1 days at 25^oC and from 21.1 to 29.8 days at 29^oC (Shepherd, Walldorf, Hug and Gehring, 1989).

Relationship to Other Genes

Source for database merge of

Source for merge of: Ef1α48D l(2)k07303

(Beaton, 1999.12.12)

Source for merge of: Ef1α48D l(2)00248

(Kahsai et al., 2016)

Additional comments

The Ef1α48D gene may have been derived from the Ef1α100E gene by retroposition.

(Bai et al., 2007, Langille and Clark, 2007, Langille and Clark, 2007)

The Ef1α48D gene may be derived from the Ef1α100E gene by retroposition.

(Betran et al., 2002)

"l(2)00248" probably corresponds to "Ef1α48D"; the P{PZ} insertion in the "l(2)00248⁰⁰²⁴⁸" allele maps only 42bp upstream of the transcription start of the CT24517 transcript of "Ef1α48D".

(Misra, 2000.8.25)

Not allelic to shot.

(Gregory and Brown, 1998)

Nomenclature History

Source for database identify of

Source for identity of: eEF1α1 Ef1α48D

(Marygold et al., 2017)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (60)

Reported As

Symbol Synonym

0248

(Harvie et al., 1998)

1275

(Harvie et al., 1998)

Dm0084

(Teeter et al., 2000)

DmEF1

(Grinblat et al., 1989)

(Guichard et al., 2006, Giribet et al., 2001)

EF-1

(Arking et al., 1996)

EF-1^a

(Harvie and Bryant, 1997)

EF-1a

(Khan et al., 2019, Shikama et al., 1992)

EF-1α

(Tycowski and Steitz, 2001, Johnson, 1997, Kaiser et al., 1997, Ackermann and Brack, 1996, Rose and Archer, 1996, Shmookler Reis and Ebert, 1996, Stearns and Kaiser, 1996, Tower, 1996, Gasser et al., 1995, Ackermann et al., 1994, Dudas and Arking, 1994, Nusbaum and Rose, 1994, Stearns and Kaiser, 1993, Stearns et al., 1993, Shepherd et al., 1989)

EF-1α F1

(Shikama and Brack, 1996)

EF-1αF

(Liang and Biggin, 1998)

EF-1αF1

(Kutach and Kadonaga, 2000)

EF-1αF₁

(Harvie et al., 1998)

EF1

(Chen et al., 2021)

EF1-alpha

(Hilger et al., 2009)

EF1-α

(Rose, 1999)

EF11_DROME

(Fukuyama et al., 2012)

EF1A1

(Eikenes et al., 2015)

EF1α

(Busto et al., 2016, Fan et al., 2010, van Steensel et al., 2001, Leips and Mackay, 2000, Takada et al., 2000, Tatar, 1999)

EF1α 48D

(Fan et al., 2010)

EF1α1

(Zolotarev et al., 2016)

EF1α48D

(Worringer et al., 2009, Jasper et al., 2002)

Ef1

(Erokhin et al., 2013)

Ef1a10

(Kliman and Eyre-Walker, 1998)

Ef1a48D

(Thomson et al., 2008)

Ef1alpha 48D

(Stephano et al., 2018, Pathak et al., 2014)

Ef1alpha48E

(Betran et al., 2002)

Ef1α1

Ef1α48D

(Wong et al., 2023, Li et al., 2017, Abruzzi et al., 2011, Ling and Salvaterra, 2011, Hoopfer et al., 2008, Bai et al., 2007, Dietzl et al., 2007, Marygold et al., 2007, Stuart et al., 2007, Kulkarni et al., 2006)

Ef1α48E

(Betran et al., 2002)

Efalpha48D

(Cermelli et al., 2006)

(Shepherd et al., 1989, Henikoff and Wallace, 1988)

F1 EF-1α

(Donati et al., 1996)

anon-EST:Liang-1.17

anon-EST:Liang-2.46

chr2R:7409490..7409690

(Will et al., 2013)

chr2R:7410009..7410129

(Will et al., 2013)

clone 1.17

(Liang and Biggin, 1998)

clone 2.46

(Liang and Biggin, 1998)

eEF1a1

(Silva et al., 2021)

eEF1α

(Lasko, 2000)

eEF1α1

(Takada et al., 2025, De Groef et al., 2024, Kaneko et al., 2024, Ramesh et al., 2023, Majane et al., 2022, Bilder et al., 2021, Vásquez-Procopio et al., 2020, Liaw and Chiang, 2019, Bost et al., 2018, Marygold et al., 2017)

ef-1α

(Wang et al., 2004)

l(2)00248

(Kahsai et al., 2016, McClure and Schubiger, 2008)

l(2)01275

l(2)1275

(Harvie, 1997.6.4)

l(2)248

(Gregory and Brown, 1998)

l(2)k07303

(BDGP Project Members, 1994-1999)

Name Synonyms

Ef1 alpha 48D

(Wójtowicz et al., 2015)

Elongation factor 1 alpha

(Ling and Salvaterra, 2011)

Elongation factor 1 alpha 48D

(Cermelli et al., 2006)

Elongation factor 1alpha48D

(Jaspers et al., 2012, Cammarato et al., 2011)

Elongation factor 1α48D

(Costa et al., 2013, Erokhin et al., 2013)

Translational elongation factor1

(Kang et al., 2002)

elongation factor 1-alpha F1

(Hovemann, 1988.2.18)

elongation factor 1alpha48D

(Sen and Cox, 2016, Hull et al., 2015)

eukaryotic translation elongation factor 1 alpha 1

lethal (2) 00248

Secondary FlyBase IDs

FBgn0000556
FBgn0010480
FBgn0022092
FBgn0010459

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 68 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

36271

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P08736

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P08736

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q8T3U3

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P08736

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36271/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0284245

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0284245

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG8280

MARRVEL_MODEL - MARRVEL (model organism gene)

36271

Linkouts

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0000556

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0284245

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8280

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC031771

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Nucleocytoplasmic transport

MIST (protein-protein) - An integrated Molecular Interaction Database

36271

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (198)

The following external sites may use different assemblies or annotations than FlyBase.

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