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Gene Dmel\sif

General Information
Symbol	Dmel\sif	Species	D. melanogaster
Name	still life	Annotation symbol	CG34418
Feature type	protein_coding_gene	FlyBase ID	FBgn0085447
Gene Model Status	Current	Stock availability	18 publicly available
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	64E1-64E5	Sequence location	3L:5,660,920..5,741,139 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene still life is referred to in FlyBase by the symbol Dmel\sif (CG34418, FBgn0085447). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: Rho guanyl-nucleotide exchange factor activity. There is experimental evidence that it is involved in the biological process: regulation of axonogenesis. 19 alleles are reported. The phenotypes of these alleles are annotated with: bouton; mesothoracic tergum. It has 8 annotated transcripts and 8 annotated polypeptides. Protein features are: Dbl homology (DH) domain; EVH1; Guanine-nucleotide dissociation stimulator, CDC24, conserved site; PDZ/DHR/GLGF; Pleckstrin homology domain; Pleckstrin homology-type; Raf-like Ras-binding. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of extremely low expression. Peak expression observed within 12-24 hour embryonic stages, during early larval stages, during late pupal stages, in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression in all larval and adult organs/tissues ranges from low to undetected. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system. Gene sequence location is 3L:5660920..5741139.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01	Sequence features BKN33323 BKN28810 HFA11395 HFA08759
FB2011_10	References Georgiou and Baum, 2010, J. Cell Sci. 123(7): 1089--1098 Bakal et al., 2007, Science 316(5832): 1753--1756
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 64E1-64E5   Limits computationally determined from genome sequence between P{PZ}sinu06524 and P{PZ}Bre101640
Experimentally Determined Cytological Location
Cytogenetic map Notes References 64E-64E     (Sone, 1996.7.15) 64E-64E   (determined by in situ hybridisation)   (Sone et al., 1997)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\sif for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model includes transcripts encoding non-overlapping portions of the full CDS Gene merge based on conservation across Drosophila species (region between former annotations shows pattern of conservation consistent with protein coding region).
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) sif-RA FBtr0112709  NM_079908   9271   2052 sif-RB FBtr0112708  NM_168126   7857   2072 sif-RC FBtr0112710  NM_206278   9298   2061 sif-RD FBtr0112711  NM_001104046   9910   2682 sif-RE FBtr0112714  NM_001104049   7939   2025 sif-RF FBtr0112712  NM_001104047   8535   2657 sif-RG FBtr0112713  NM_001104048   8713   2408 sif-RH FBtr0114646  NM_001144420   687   154
Additional Transcript Data & Comments
Reported size (kB)	9.233, 7.854 (compiled cDNA) (Sone et al., 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein sif-PA   FBpp0111621   229.0   2052   8.99     NP_524647   AAF50756 sif-PB   FBpp0111620   231.1   2072   8.07     NP_729084   AAF50755 sif-PC   FBpp0111622   229.9   2061   8.99     NP_996000   AAS65075 sif-PD   FBpp0111623   297.8   2682   8.49     NP_001097516   ABW08472 sif-PE   FBpp0111626   225.8   2025   8.71     NP_001097519   ABW08474 sif-PF   FBpp0111624   295.3   2657   7.86     NP_001097517   AAN12103 sif-PG   FBpp0111625   264.0   2408   8.77     NP_001097518   ABW08473 sif-PH   FBpp0113138   17.4   154   8.05     NP_001137892   ACL83247
Additional Polypeptide Data & Comments
Reported size (kDa)	2064, 2044 (aa) (Sone et al., 1997)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Dbl homology (DH) domain (IPR000219) Guanine-nucleotide dissociation stimulator, CDC24, conserved site (IPR001331) EVH1 (IPR000697) Pleckstrin homology domain (IPR001849) Pleckstrin homology-type (IPR011993) PDZ/DHR/GLGF (IPR001478) Raf-like Ras-binding (IPR003116)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA567950   AE014296 AAF50755   AE014296 AAF50756   AE014296 AAN12103   AE014296 AAS65075   AE014296 ABW08472   AE014296 ABW08473   AE014296 ABW08474   AE014296 ACL83247   AI113562   AI113856   AI113857   AI402203   AY089550   AY118305 AAM48334   BE978109   BI608936   BI608978   BI617503   BT024945   BT120133   BZ286328   BZ286329   CO177407   CO178174   CO178388   CO186247   CO187793   CO190293   CO262302   CO268087   CO268220   CO268221   CO272478   CO275127   CO277067   CO279703   CO281457   CO282423   CO287334   CO287820   CO287913   CO288836   CO291029   CO291887   CO293305   CO298079   CO300313   CO300681   CO309399   CO310608   CO314615   CO320112   CO321536   CO325328   CO328285   CO328372   CO333497   CO343000   CZ471511   CZ473093   CZ485589   D86546 BAA13108   D86547 BAA13109   EC059411   EC069729   EC070541   EC072485   EC073639   EC077262   EC078606   EC079381   EC082465   EC083592   EC084356   EC085868   EC086196   EC196258   EC196259   EC199605   EC202912   EC203532   EC207153   EC208022   EC209100   EC218925   EC219373   EC220575   EC220705   EC221132   EC221681   EC221796   EC222336   EC222882   EC223134   EC225133   EC229902   EC231392   EC233844   EC235329   EC235491   EC236429   EC240417   EC240768   EC242823   EC242824   EC247127   EC260550   EL874691   EL885016   EL885141   FE527413   FE527469   FE527522   FE527530   FE527584   FL502771   FL502776
UniProtKB/Swiss-Prot	P91621 P91620
UniProtKB/TrEMBL	B7Z0C2 A8JNL5 A8JNL4 A8JNL6
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 14   presumptive embryonic/larval central nervous system | restricted   (Sone et al., 1997) presumptive embryonic/larval central nervous system | segmentally repeated   (Sone et al., 1997) embryonic stage 17   ventral nerve cord   (Sone et al., 1997) embryonic brain   (Sone et al., 1997)
Additional Descriptive Data
	In stage 14 embryos, sif transcripts are expressed in several cells per segment in the CNS. At stage 17, expression is observed exclusively in the brain and ventral nerve cord. (Sone et al., 1997) In stage 14 embryos, sif transcripts are expressed in several cells per segment in the CNS. At stage 17, expression is observed exclusively in the brain and ventral nerve cord. (Sone et al., 1997)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 17   ventral nerve cord   (Sone et al., 1997) third instar larval stage   bouton of neuromuscular junction   (Sone et al., 1997) adult stage   medulla neuropil   (Sone et al., 1997) lobula plate   (Sone et al., 1997) lamina neuropil   (Sone et al., 1997) mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
	sif protein is localized to limited cytoplasmic regions of photoreceptor axon terminals in the adult optic lobe. It is closely associated with the plasma membrane especially in regions where the membrane protrudes outward toward other cells. In the larval neuromuscular junction, staining is found adjacent to the plasma membrane of presynaptic nerve terminals. (Sone et al., 1997) sif protein is located in neuropil of the ventral nerve cord in stage 17 embryos. In third instar larvae, it is detected in body wall muscle in the boutons of neuromuscular junctions. sif protein is also found in neuropil in the adult brain. Thus, it is exclusively localized to synaptic regions primarily after the synapses have undergone maturation. (Sone et al., 1997)
Marker for
Subcellular Localization
CV Term	cytoplasm (Sone et al., 1997)
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0085447    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0085447 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of extremely low expression. Peak expression observed within 12-24 hour embryonic stages, during early larval stages, during late pupal stages, in adult male stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0085447 expression level Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    2 embryo 04-06hr    6 embryo 06-08hr    21 embryo 08-10hr    32 embryo 10-12hr    51 embryo 12-14hr    171 embryo 14-16hr    788 embryo 16-18hr    942 embryo 18-20hr    1006 embryo 20-22hr    982 embryo 22-24hr    920 larva L1    1113 larva L2    323 larva L3 12hr old    112 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6    83 larva L3 puffstage 7-9    66 white prepupae new    58 white prepupae 12hr    154 white prepupae 24hr    285 pupae 2d postWPP    776 pupae 3d postWPP    1202 pupae 4d postWPP    815 adult male 01day    791 adult male 05day    850 adult male 30day    837 adult female 01day    370 adult female 05day    94 adult female 30day    123 Expression Level Scale  None   Extremely low   Very low   Low   Moderate  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    2 embryo 04-06hr    6 embryo 06-08hr    21 embryo 08-10hr    32 embryo 10-12hr    51 embryo 12-14hr  (171) embryo 14-16hr  (788) embryo 16-18hr  (942) embryo 18-20hr  (1006) embryo 20-22hr  (982) embryo 22-24hr  (920) larva L1  (1113) larva L2  (323) larva L3 12hr old  (112) larva L3 puffstage 1-2    24 larva L3 puffstage 3-6    83 larva L3 puffstage 7-9    66 white prepupae new    58 white prepupae 12hr  (154) white prepupae 24hr  (285) pupae 2d postWPP  (776) pupae 3d postWPP  (1202) pupae 4d postWPP  (815) adult male 01day  (791) adult male 05day  (850) adult male 30day  (837) adult female 01day  (370) adult female 05day    94 adult female 30day  (123) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    2 embryo 04-06hr    6 embryo 06-08hr    21 embryo 08-10hr    32 embryo 10-12hr    51 embryo 12-14hr    171 embryo 14-16hr    788 embryo 16-18hr    942 embryo 18-20hr    1006 embryo 20-22hr    982 embryo 22-24hr    920 larva L1    1113 larva L2    323 larva L3 12hr old    112 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6    83 larva L3 puffstage 7-9    66 white prepupae new    58 white prepupae 12hr    154 white prepupae 24hr    285 pupae 2d postWPP    776 pupae 3d postWPP    1202 pupae 4d postWPP    815 adult male 01day    791 adult male 05day    850 adult male 30day    837 adult female 01day    370 adult female 05day    94 adult female 30day    123 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    2 embryo 04-06hr    6 embryo 06-08hr    21 embryo 08-10hr    32 embryo 10-12hr    51 embryo 12-14hr    171 embryo 14-16hr    788 embryo 16-18hr    942 embryo 18-20hr    1006 embryo 20-22hr    982 embryo 22-24hr    920 larva L1    1113 larva L2    323 larva L3 12hr old    112 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6    83 larva L3 puffstage 7-9    66 white prepupae new    58 white prepupae 12hr    154 white prepupae 24hr    285 pupae 2d postWPP    776 pupae 3d postWPP    1202 pupae 4d postWPP    815 adult male 01day    791 adult male 05day    850 adult male 30day    837 adult female 01day    370 adult female 05day    94 adult female 30day    123 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    2 embryo 04-06hr    6 embryo 06-08hr    21 embryo 08-10hr    32 embryo 10-12hr    51 embryo 12-14hr    171 embryo 14-16hr    788 embryo 16-18hr    942 embryo 18-20hr    1006 embryo 20-22hr    982 embryo 22-24hr    920 larva L1    1113 larva L2    323 larva L3 12hr old    112 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6    83 larva L3 puffstage 7-9    66 white prepupae new    58 white prepupae 12hr    154 white prepupae 24hr    285 pupae 2d postWPP    776 pupae 3d postWPP    1202 pupae 4d postWPP    815 adult male 01day    791 adult male 05day    850 adult male 30day    837 adult female 01day    370 adult female 05day    94 adult female 30day    123 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0085447 expression level Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    2 embryo 04-06hr    6 embryo 06-08hr    21 embryo 08-10hr    32 embryo 10-12hr    51 embryo 12-14hr    171 embryo 14-16hr    788 embryo 16-18hr    942 embryo 18-20hr    1006 embryo 20-22hr    982 embryo 22-24hr    920 larva L1    1113 larva L2    323 larva L3 12hr old    112 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6    83 larva L3 puffstage 7-9    66 white prepupae new    58 white prepupae 12hr    154 white prepupae 24hr    285 pupae 2d postWPP    776 pupae 3d postWPP    1202 pupae 4d postWPP    815 adult male 01day    791 adult male 05day    850 adult male 30day    837 adult female 01day    370 adult female 05day    94 adult female 30day    123 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    2 embryo 04-06hr    6 embryo 06-08hr    21 embryo 08-10hr    32 embryo 10-12hr    51 embryo 12-14hr  (171) embryo 14-16hr  (788) embryo 16-18hr  (942) embryo 18-20hr  (1006) embryo 20-22hr  (982) embryo 22-24hr  (920) larva L1  (1113) larva L2  (323) larva L3 12hr old  112 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6    83 larva L3 puffstage 7-9    66 white prepupae new    58 white prepupae 12hr  (154) white prepupae 24hr  (285) pupae 2d postWPP  (776) pupae 3d postWPP  (1202) pupae 4d postWPP  (815) adult male 01day  (791) adult male 05day  (850) adult male 30day  (837) adult female 01day  (370) adult female 05day    94 adult female 30day  123 Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    2 embryo 04-06hr    6 embryo 06-08hr    21 embryo 08-10hr    32 embryo 10-12hr    51 embryo 12-14hr    171 embryo 14-16hr    788 embryo 16-18hr    942 embryo 18-20hr    1006 embryo 20-22hr    982 embryo 22-24hr    920 larva L1    1113 larva L2    323 larva L3 12hr old    112 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6    83 larva L3 puffstage 7-9    66 white prepupae new    58 white prepupae 12hr    154 white prepupae 24hr    285 pupae 2d postWPP    776 pupae 3d postWPP    1202 pupae 4d postWPP    815 adult male 01day    791 adult male 05day    850 adult male 30day    837 adult female 01day    370 adult female 05day    94 adult female 30day    123 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    2 embryo 04-06hr    6 embryo 06-08hr    21 embryo 08-10hr    32 embryo 10-12hr    51 embryo 12-14hr    171 embryo 14-16hr    788 embryo 16-18hr    942 embryo 18-20hr    1006 embryo 20-22hr    982 embryo 22-24hr    920 larva L1    1113 larva L2    323 larva L3 12hr old    112 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6    83 larva L3 puffstage 7-9    66 white prepupae new    58 white prepupae 12hr    154 white prepupae 24hr    285 pupae 2d postWPP    776 pupae 3d postWPP    1202 pupae 4d postWPP    815 adult male 01day    791 adult male 05day    850 adult male 30day    837 adult female 01day    370 adult female 05day    94 adult female 30day    123 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    2 embryo 04-06hr    6 embryo 06-08hr    21 embryo 08-10hr    32 embryo 10-12hr    51 embryo 12-14hr    171 embryo 14-16hr    788 embryo 16-18hr    942 embryo 18-20hr    1006 embryo 20-22hr    982 embryo 22-24hr    920 larva L1    1113 larva L2    323 larva L3 12hr old    112 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6    83 larva L3 puffstage 7-9    66 white prepupae new    58 white prepupae 12hr    154 white prepupae 24hr    285 pupae 2d postWPP    776 pupae 3d postWPP    1202 pupae 4d postWPP    815 adult male 01day    791 adult male 05day    850 adult male 30day    837 adult female 01day    370 adult female 05day    94 adult female 30day    123 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0085447    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0085447 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression in all larval and adult organs/tissues ranges from low to undetected. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Heatmap Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut     Larval Hindgut no informative data Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass no informative data Adult Head no informative data Adult Eye no informative data Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut     Adult Hindgut no informative data Adult Malpighian Tubules     Adult Fat Body no informative data Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary     Adult Testis     Adult Male Accessory Gland no informative data Adult Carcass no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_14
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0085447 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG32414-RA;CG5406-RB
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 sifGD10887 sifGD11638 Sterility Allele male sterile | recessive sif98.1 Other Phenotypes Allele locomotor behavior defective | recessive sif98.1 sifES11 neuroanatomy defective (with Df(3L)64D-F) sifES11 visible, with Scer\GAL4pnr-MD237 sifGD10887 wild-type sifelav.PS Phenotype manifest in Allele bouton (with Df(3L)64D-F) sifES11 chaeta | supernumerary, with Scer\GAL4pnr-MD237 sifGD10887 mesothoracic tergum, with Scer\GAL4pnr-MD237 sifGD10887
Classical Alleles ( 13 )
For All Classical Alleles Show
Allele of sif Class Mutagen Stocks Known lesion sifES11 ethyl methanesulfonate 2 Yes sifd00298 P-element activity 1 -- sifd02948 P-element activity 1 -- siff03033 piggyBac transposase 1 -- sifHP30448 1 -- sifKG09067 P-element activity 1 -- sifMB00266 1 -- sifMB01486 1 -- sifMB02755 1 -- sifMI02376 1 -- sif98.1 P-element activity 0 Yes sifA 0 -- sifd08550 0 --
Alleles Carried on Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Allele of sif Class Mutagen Stocks Known lesion sifGD11638 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes sifScer\UAS.cSa in vitro construct - regulatory fusion 2 Yes sifGD10887 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes sifJF01795 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes sifKK108810 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes sifelav.PS in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)SseA (Jager et al., 2001)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD10887} NA P{GD11638} NA P{KK108810} NA P{TRiP.JF01795} NA P{UAS-sif.S} NA characterization construct P{elav-sif} NA P{SIFΔN} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions Mi{MIC}sifMI02376 NA P{}sif98.1 NA P{SUPor-P}sifKG09067 NA PBac{WH}siff03033 NA insertion of mobile activating element P{EPg}sifHP30448 NA P{XP}sifd00298 NA P{XP}sifd02948 NA P{XP}sifd08550 NA insertion of enhancer trap binary system Mi{ET1}sifMB00266 No Mi{ET1}sifMB01486 No Mi{ET1}sifMB02755 No
Gene Ontology: Function, Process & Cellular Component ( 10 unique terms )
Terms Based on Experimental Evidence ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
regulation of axonogenesis inferred from genetic interaction with LIMK1 (Ng and Luo, 2004)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 9 terms )
Molecular Function
CV term Evidence References
guanyl-nucleotide exchange factor activity non-traceable author statement (Sone, 1996.7.15) receptor signaling protein activity inferred from electronic annotation with InterPro:IPR003116 (FlyBase Curators et al., 2004-) Rho guanyl-nucleotide exchange factor activity inferred from electronic annotation with InterPro:IPR000219 (FlyBase Curators et al., 2004-) inferred from sequence or structural similarity (Sone et al., 1997)
Biological Process
CV term Evidence References
actin cytoskeleton organization non-traceable author statement (Swiss-Prot Project Members, 1999.4.15, Swiss-Prot Project Members, 1999.4.15) regulation of Rho protein signal transduction inferred from electronic annotation with InterPro:IPR000219 (FlyBase Curators et al., 2004-) regulation of synapse structure and activity traceable author statement (Packard et al., 2003) synaptic transmission non-traceable author statement (Swiss-Prot Project Members, 1999.4.15, Swiss-Prot Project Members, 1999.4.15)
Cellular Component
CV term Evidence References
intracellular inferred from electronic annotation with InterPro:IPR000219 (FlyBase Curators et al., 2004-) synapse non-traceable author statement (Swiss-Prot Project Members, 1999.4.15, Swiss-Prot Project Members, 1999.4.15)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions sif allele Gene References sifES11 Fas2 (Sone et al., 2000) LIMK1 (Ng and Luo, 2004) sifScer\UAS.cSa Fas2 (Sone et al., 2000) LIMK1 (Ng and Luo, 2004)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 68698 DroID - A comprehensive database of gene and protein interactions. • FBgn0085447 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43892
Orthologs
Genome-wide drosophilid orthologs
Curated drosophilid orthologs	Dpse\GA16886   Dpse\GA18858
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL004246-PB Aedes aegypti (mosquito) AGAP006590-PA Anopheles gambiae (mosquito) BGIBMGA000379-PA Bombyx mori (silk worm) CPIJ014866 Culex pipens (mosquito) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV16161 LH23788 FBgn0238761 FBgn0219303 FBgn0200641 FBgn0185592 FBgn0169636 JGI_V11_104151 FBgn0078858 FBgn0149964 FBgn0134895 FBgn0123937 FBgn0107534 FBgn0100982 CPIJ014866 BGIBMGA000379 GB10237 AGAP006590 AAEL004246 PB22232 PHUM606710 GLEAN_13483
Stocks & Reagents
Stocks Listed in FlyBase ( 18 )
Bloomington	9127 w*; P{UAS-sif.S}M3.1 24030 w1118; Mi{ET1}sifMB02755 25789 y1 v1; P{TRiP.JF01795}attP2 22749 y1 w67c23; Mi{ET1}sifMB00266 23324 y1 w67c23; Mi{ET1}sifMB01486
Harvard	- P{XP}sifd00298 - P{XP}sifd02948 - PBac{WH}siff03033
VDRC	v106832 P{KK108810}VIE-260B v26132 w1118 P{GD10887}v26132 v27406 w1118; P{GD11638}v27406 v27407 w1118; P{GD11638}v27407/TM3
	More stocks available...
Genomic Clones ( 3 )
	BACR11E06 BACR13A12 BACR29P05 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 93 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH10341 IP16015 LD26837
Other clones	IP16115
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH09902 GH10342 HL02037 RH13656 RH13704 RH47860
Other clones	1496 1682 8648 14470 15472 28290 31718 33637 33708 40549 42433 45082 46977 54100 57531 60853 65207 67253 70763 73327 83582 99335 117768 144287 147630 177213 214469.19 281773 bs74a04 CK01.17C.G8 CK01.24B.G4 CK01.58D.C3 CK01.65C.H2 CK01.69C.H4 CK01.84C.C12 CK01.88A.G8 CK01.88A.H8 CK01.98A.E8 CK01.100D.C11 CK01.107D.A2 CK01.107D.A8 CK01.118D.G5 DMG3-chr3L.5910.pred.a DMG4-chr3L.10.010.pred.a EK017660 EK033508 EK047710 EK053361 EK055562 EK056608 EK065562 EK066907 EK067133 EK069460 EK075852 EK085012 EK090127 EK090633 EK090636 EK107323 EK116530 EK122842 EK135707 EK157217 EK160440 EK171324 EK177922 EK179055 EK185855 EK196131 EK198614 EK202528 EK217518 EK222117 EK237153 EK282261 EK307115 EP08914 EP09101 EP15911 IP14806 IP16215 IP16315
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0085447 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43892 318019
Antibody Information
	polyclonal (Sone et al., 1997)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: sif CG5256 (Gabrielian, 1999.11) Source for identity of: sif CG5406 (Gabrielian, 1999.11)
Source for database merge of	Source for merge of: sif CG5256 CG5406 (Bayraktaroglu, 2000.4.17) Source for merge of: sif CG32414 (FlyBase Genome Annotators, 2007)
Additional comments	Annotations CG5406 and CG32414 merged as CG34418 in release 5.2 of the genome annotation. (FlyBase Genome Annotators, 2007)
Other Comments
	New annotation (CG32414) in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003) Expression of a truncated sif protein results in defects in neuronal morphology and induces membrane ruffling with altered actin localisation in human KB cells. (Sone et al., 1997) sif may regulate the change of axon terminal morphology with differentiation into mature synapses. (Sone et al., 1997) The sif gene product may regulate synaptic differentiation through the organization of the actin cytoskeleton by activating Rho-like GTPases. (Sone et al., 1997)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079908 NM_168126 NM_206278 NM_001104046 NM_001104047 NM_001104048 NM_001104049 NM_001144420 RefSeq (Proteins) • NP_524647 NP_729084 NP_996000 NP_001097516 NP_001097517 NP_001097518 NP_001097519 NP_001137892 DDBJ / EMBL / GenBank • AAF50755 AAF50756 AAM48334 AAN12103 AAS65075 ABW08472 ABW08473 ABW08474 ACL83247 AI113856 AI113857 AI402203 AY118305 BAA13108 BAA13109 BZ286328 BZ286329 CZ471511 CZ473093 CZ485589 D86546 D86547 Entrez Gene - A searchable database of RefSeq genes. • 43892 UniProtKB/Swiss-Prot • P91620 P91621 UniProtKB/TrEMBL • A8JNL4 A8JNL5 A8JNL6 B7Z0C2
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Dbl homology (DH) domain (IPR000219) Guanine-nucleotide dissociation stimulator, CDC24, conserved site (IPR001331) EVH1 (IPR000697) Pleckstrin homology domain (IPR001849) Pleckstrin homology-type (IPR011993) PDZ/DHR/GLGF (IPR001478) Raf-like Ras-binding (IPR003116)
Linkouts
	BioGRID - A database of protein and genetic interactions • 68698 DroID - A comprehensive database of gene and protein interactions. • FBgn0085447 DRSC - Results from RNAi screens. • FBgn0085447 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0085447 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG32414-RA;CG5406-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0085447 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43892 318019 InParanoid A subset of ortholog calls from InParanoid. • AAEL004246-PB Aedes aegypti (mosquito) AGAP006590-PA Anopheles gambiae (mosquito) BGIBMGA000379-PA Bombyx mori (silk worm) CPIJ014866 Culex pipens (mosquito) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43892 modMine - Data generated by the modENCODE project. • FBgn0085447 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV16161 LH23788 FBgn0238761 FBgn0219303 FBgn0200641 FBgn0185592 FBgn0169636 JGI_V11_104151 FBgn0078858 FBgn0149964 FBgn0134895 FBgn0123937 FBgn0107534 FBgn0100982 CPIJ014866 BGIBMGA000379 GB10237 AGAP006590 AAEL004246 PB22232 PHUM606710 GLEAN_13483
Synonyms & Secondary IDs ( 16 )
Reported As
Symbol Synonym	CG5256   CG5406 (Mount, 2001.8.14, ) CG32414   CG34418 (FlyBase Genome Annotators, 2007, Perkins et al., 1.15.2009, Keleman et al., 2009.8.5) RhoGEF (Hanai et al., 2002) SIF (Jin, 2002, Sone et al., 2001, Bakal et al., 2007, Bakal et al., 2007) sif (Peyre et al., 2006, Bakal et al., 2008, Ng, 2008, Dickman and Davis, 2009, Glazov et al., 2005, Wasbrough et al., 2010, Ball et al., 2010) Sif (Ball et al., 2010, Oliva and Sierralta, 2010, Georgiou and Baum, 2010) slf (Ponting et al., 1997) unnamed (Hicks et al., 1997)
Name Synonym	CG32414   Still life (Hanai et al., 2002) still life (Sone et al., 1997, Wolfgang et al., 2007, Glazov et al., 2005) still-life (Bakal et al., 2008) Still Life (Oliva and Sierralta, 2010) still life type 1 (Sone, 1996.7.15) still life type 2 (Sone, 1996.7.15)
Secondary FlyBase IDs
	FBan0032414 FBgn0019652 FBgn0019656 FBgn0035628 FBgn0035629 FBgn0052414
References ( 65 )
Generate a list of	All references relating to this gene ( 65 )
List References by type	Research paper  ( 16 ) Supplementary material  ( 5 ) Review  ( 10 ) Erratum  ( 1 ) Personal communication to FlyBase  ( 11 ) Abstract  ( 7 ) FlyBase analysis  ( 4 ) DNA/RNA sequence record  ( 2 ) Curated genome annotation  ( 3 ) Protein sequence record  ( 2 ) Computer file  ( 3 ) Automatic genome annotation  ( 1 )
Recent research papers ( 3 )
	Ball et al., 2010, Neuron 66(4): 536--549 Retrograde BMP Signaling Controls Synaptic Growth at the NMJ by Regulating Trio Expression in Motor Neurons. [FBrf0210920] Georgiou and Baum, 2010, J. Cell Sci. 123(7): 1089--1098 Polarity proteins and Rho GTPases cooperate to spatially organise epithelial actin-based protrusions. [FBrf0214990] Oliva and Sierralta, 2010, Dev. Biol. 344(2): 911--921 Regulation of axonal development by the nuclear protein hindsight (pebbled) in the Drosophila visual system. [FBrf0211428] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...