FlyBase Gene Report: Dmel\Rcc1

General Information

Symbol

Dmel\Rcc1

Species

D. melanogaster

Name

Regulator of chromosome condensation 1

Annotation Symbol

CG10480

Feature Type

protein_coding_gene

FlyBase ID

FBgn0002638

Gene Model Status

Current

Stock Availability

12 publicly available

Gene Summary

Regulator of chromosome condensation 1 (Rcc1) encodes the guanine-nucleotide exchange factor for the product of Ran. It promotes the nucleo-cytoplasmic shuttling of specific proteins and regulates chromatin condensation. The roles of Rcc1 product include cell proliferation, cell survival, apoptosis, epigenetic regulation, and neuronal specification. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

Bj1, RanGEF, dRCC1, inxs

Key Links

Genomic Location

Cytogenetic map

65A1-65A1

Sequence location

3L:5,930,110..5,932,773 [-]

Recombination map

3-16

RefSeq locus

NT_037436 REGION:5930110..5932773

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (12 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables chromatin binding

inferred from direct assay

(Trieselmann and Wilde, 2002)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables guanyl-nucleotide exchange factor activity

inferred from sequence or structural similarity with UniProtKB:P18754

(Shi and Skeath, 2004)

Biological Process (7 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

involved_in central nervous system development

inferred from direct assay

(Frasch, 1991)

involved_in NLS-bearing protein import into nucleus

inferred from mutant phenotype

(Shi and Skeath, 2004)

involved_in regulation of mitotic cell cycle

inferred from direct assay

(Trieselmann and Wilde, 2002)

involved_in regulation of neurogenesis

inferred from mutant phenotype

(Shi and Skeath, 2004)

involved_in regulation of nucleocytoplasmic transport

inferred from mutant phenotype

(Wu et al., 2019, Shi and Skeath, 2004)

involved_in ventral cord development

inferred from high throughput mutant phenotype

(Bivik et al., 2015)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in regulation of mitotic cell cycle

inferred from biological aspect of ancestor with PANTHER:PTN002338189

(GO Reference Genome Project, 2011-)

involved_in regulation of mitotic spindle assembly

inferred from biological aspect of ancestor with PANTHER:PTN002338189

(GO Reference Genome Project, 2011-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in condensed chromosome

inferred from direct assay

(Shi and Skeath, 2004)

located_in nucleus

inferred from direct assay

(Shi and Skeath, 2004)

inferred from direct assay

(Frasch, 1991)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN002338189

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

RAN GUANINE NUCLEOTIDE EXCHANGE FACTORS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

RAN GUANINE NUCLEOTIDE EXCHANGE FACTORS -: Ran Guanine Nucleotide Exchange Factor (RanGEF) promotes the exchange of Ran-bound GDP for GTP on nucleoplasmic side of the nuclear pore as part of the nucleocytoplasmic transport shuttling cycle of Ran. (Adapted from FBrf0174533).

Protein Function (UniProtKB)

Promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds to chromatin.

(UniProt, P25171)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Rcc1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P25171)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

3MVD

Comments on Gene Model

Gene model reviewed during 5.44

Gene model reviewed during 5.45

Gene model reviewed during 6.61

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Rcc1-RA

FBtr0077074

NM_079219

2365

547

Rcc1-RB

FBtr0077075

NM_168151

2144

547

Rcc1-RC

FBtr0077076

NM_168152

2085

547

Rcc1-RD

FBtr0482688

2230

547

Additional Transcript Data and Comments

Reported size (kB)

2.2 (northern blot)

(Frasch, 1991)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Rcc1-PA

FBpp0076782

58.9

547

8.06

FBpp0076783

58.9

547

8.06

FBpp0076784

58.9

547

8.06

FBpp0428608

58.9

547

8.06

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

547 aa isoforms: Rcc1-PA, Rcc1-PB, Rcc1-PC, Rcc1-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

547 (aa); 68 (kD)

(Frasch, 1991)

68 (kD)

(Ohtsubo et al., 1991)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

3MVD

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

P25171

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rcc1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Rcc1_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.78

Transcript Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage & oogenesis

nurse cell

(Frasch, 1991)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

neuroblast

(Frasch, 1991)

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

visual anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

ventral nerve cord primordium

Comment: reported as ventral nerve cord anlage

(Fisher et al., 2012)

embryonic stage 9 -- 10

antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 11 -- 12

central brain primordium

(Fisher et al., 2012)

embryonic neuroblast of ventral nerve cord primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

procephalic neuroblast

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic central brain neuron

(Fisher et al., 2012)

embryonic central brain surface glial cell

(Fisher et al., 2012)

larval nervous system

(Fisher et al., 2012)

gonad

(Fisher et al., 2012)

lateral cord neuron

(Fisher et al., 2012)

larval lateral VNC surface glial cell

(Fisher et al., 2012)

mushroom body primordium

Comment: reported as embryonic central brain mushroom body

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

adult stage & oogenesis

oocyte

(Frasch, 1991)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 15

organism

(Frasch, 1991)

Additional Descriptive Data

Bj1 transcripts are abundant in early embryos and adult females and are barely detectable at other developmental stages. Bj1 transcripts are strongly enriched in the prospective oocyte of young follicles and only low levels are present in nurse cells. At stage 10 of oogenesis, strong expression starts in the nurse cells and the transcripts are later transported to the oocyte. Bj1 transcripts are uniformly distributed in early embryos. In later embryos, transcripts persist only in neuroblasts and ganglion cells derived from them.

(Frasch, 1991)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

presumptive embryonic/larval central nervous system

(Frasch, 1991)

adult stage & oogenesis

nurse cell

(Frasch, 1991)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

neuroblast

(Frasch, 1991)

• chromatin

Comment: interphase

(Trieselmann and Wilde, 2002)

embryonic stage 1 -- 12

cell

• nucleus

Comment: Bj1 is localized to all cell nuclei

(Shi and Skeath, 2004)

embryonic stage 13 -- 17

presumptive embryonic/larval central nervous system

• nucleus

(Shi and Skeath, 2004)

larval stage

embryonic/larval salivary gland

(Frasch, 1991)

adult stage | female

oocyte

(Cesario and McKim, 2011)

adult stage & oogenesis

oocyte

(Frasch, 1991)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Bj1 protein is found exclusively in nuclei in the oocyte and is predominantly localized to nuclei in early cleavage stage embryos. From the blastoderm stage onwards, Bj1 protein is exclusively nuclear during interphase. During mitosis, it becomes evenly distributed throughout the cells. It is found in all bands of polytene chromosomes and is absent from developmental puffs, heat-shock puffs and interbands.

(Cesario and McKim, 2011, Frasch, 1991)

Bj1 is maternally supplied to early embryos; the maternally-supplied protein can be detected in the nuclei of late-stage Bj1-null embryos. In wild-type embryos, Bj1 protein expression remains high in the CNS after embryonic stage 13, but is sharply decreased in other tissues. Bj1 is found exclusively in the nucleus during interphase, but colocalizes with mitotic chromosomes and forms spindle-like structures during mitosis.

(Shi and Skeath, 2004)

Bj1 (RCC1) protein wasassociated with chromatin throughout the cell cycle including during mitosis in methanol fixed embryos and as monitored by injected rhodamine labeled human RCC1 protein.

(Trieselmann and Wilde, 2002, Frasch, 1991)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in condensed chromosome

inferred from direct assay

(Shi and Skeath, 2004)

located_in nucleus

inferred from direct assay

(Shi and Skeath, 2004)

inferred from direct assay

(Frasch, 1991)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Rcc1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Rcc1

View exonic expression by tissue for Dmel\Rcc1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0002638

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38669/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0002638

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0002638

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0002638

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10480

Flygut - An atlas of the Drosophila adult midgut

FBgn0002638

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Rcc1

Stages(s) 1-3
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 17 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 12 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Rcc1

Transgenic constructs containing regulatory region of Rcc1

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3L)Exel7210

(Ryder, 2004.4.26)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

cell lethal | somatic clone

Rcc1^unspecified

lethal, with Scer\GAL4^insc-Mz1407

Rcc1^GD6989

lethal | germline clone

Rcc1^unspecified

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

Rcc1^GD6989

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Rcc1^GD6989

viable

Rcc1^EY06262

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Rcc1^GD6989

viable, with Scer\GAL4^tin.CΔ4

Rcc1^GD6989

Sterility

Allele

fertile

Rcc1^EY06262

Other Phenotypes

Allele

abnormal neuroanatomy

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav-C155

Rcc1^EP3630

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

Rcc1^GD6989

abnormal oxidative stress response | adult stage, with Scer\GAL4^elav.PU

Rcc1^GD6989

decreased rate of movement, with Scer\GAL4^elav.PU

Rcc1^GD6989

increased mortality | chemical conditional, with Scer\GAL4^elav.PU

Rcc1^GD6989

some die during pupal stage, with Scer\GAL4^pnr-MD237

Rcc1^GD6989

visible, with Scer\GAL4^pnr-MD237

Rcc1^GD6989

Phenotype manifest in

Allele

anterior fascicle & synapse, with Scer\GAL4^elav-C155

Rcc1^EP3630

apterous-expressing neuron of the lateral cluster of the thorax

apterous-expressing neuron of the lateral cluster of the thorax Ap4

central nervous system | lateral

central nervous system | lateral (with Df(3L)ZN47)

chromosome | adult stage | female, with Scer\GAL4^{VP16.mat.αTub67C}

Rcc1^UASp.mCherry

eye

Rcc1^FF32

eye photoreceptor cell

Rcc1^FF32

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

Rcc1^GD6989

larval EL neuron

larval EL neuron (with Df(3L)ZN47)

larval intersegmental nerve, with Scer\GAL4^elav-C155

Rcc1^EP3630

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

Rcc1^GD6989

mesonotum, with Scer\GAL4^pnr-MD237

Rcc1^GD6989

ommatidium

Rcc1^FF32

oocyte, with Scer\GAL4^{VP16.mat.αTub67C}

Rcc1^UASp.mCherry

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (31)

Hsap\RCC1

13 of 14

Yes

Hsap\HERC1

4 of 14

Yes

4 of 14

3 of 14

3 of 14

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (19)

Rnor\Rcc1

13 of 14

Yes

Rnor\Rpgr

4 of 14

Yes

Rnor\Herc2

3 of 14

Rnor\Rcbtb1

3 of 14

Yes

Rnor\Rcbtb2

3 of 14

Yes

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Nek8

1 of 14

Rnor\Zzef1

1 of 14

Yes

Mus musculus (laboratory mouse) (21)

Mmus\Rcc1

13 of 14

Yes

Mmus\Rpgr

4 of 14

Yes

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Mmus\Rcbtb2

3 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (30)

Xtro\rcc1

10 of 13

Yes

Xtro\herc1

2 of 13

Yes

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\pptc7

1 of 13

Xtro\rcbtb1

1 of 13

Yes

Xtro\rcbtb2

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Danio rerio (Zebrafish) (25)

Drer\rcc1

13 of 14

Yes

Drer\rpgrb

4 of 14

Yes

Drer\herc1

3 of 14

Drer\rcbtb1

3 of 14

Yes

Drer\rcbtb2

3 of 14

Yes

Drer\rcc2

3 of 14

Drer\rpgra

3 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\nek8

1 of 14

Drer\nek9

1 of 14

Yes

Drer\rccd1

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (8)

Cele\ran-3

13 of 14

Yes

3 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Yes

Cele\W09G3.7

1 of 14

Cele\wwp-1

1 of 14

Anopheles gambiae (African malaria mosquito) (14)

Agam\AgaP_AGAP003742

10 of 12

Yes

3 of 12

3 of 12

3 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (26)

Atha\AT3G02510

6 of 13

Yes

Atha\AT5G16040

6 of 13

Yes

Atha\UVR8

5 of 13

Yes

Atha\RUG1

4 of 13

Yes

Atha\RUG3

4 of 13

Yes

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

Yes

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

Yes

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (4)

Scer\SRM1

12 of 13

Yes

Scer\ATS1

1 of 13

Scer\FMP25

1 of 13

Scer\HUL4

1 of 13

Schizosaccharomyces pombe (Fission yeast) (4)

Spom\pim1

10 of 12

Yes

Spom\btb1

1 of 12

Spom\pof9

1 of 12

Yes

Spom\SPAC10F6.04

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Rcc1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (16)

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 4 )

Human Ortholog

Disease

Evidence

References

RPGR; retinitis pigmentosa GTPase regulator

model of retinitis pigmentosa 3

IEA

(FlyBase, 2019-)

model of X-linked cone-rod dystrophy 1

IEA

(FlyBase, 2019-)

model of X-linked retinitis pigmentosa and sinorespiratory infections

IEA

(FlyBase, 2019-)

model of X-linked atrophic macular degeneration

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Rcc1^KK100628

ameliorates frontotemporal dementia and/or amyotrophic lateral sclerosis 1

modeled by Zzzz\poly-PR^{25.UAS.Tag:FLAG}

(Boeynaems et al., 2016)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RCC1; regulator of chromosome condensation 1

13 of 14

179710

INFECTION-INDUCED ACUTE-ONSET AXONAL NEUROPATHY; IIAAN

HERC1; HECT and RLD domain containing E3 ubiquitin protein ligase family member 1

4 of 14

605109

MACROCEPHALY, DYSMORPHIC FACIES, AND PSYCHOMOTOR RETARDATION; MDFPMR

SERGEF; secretion regulating guanine nucleotide exchange factor

4 of 14

606051

HERC2; HECT and RLD domain containing E3 ubiquitin protein ligase 2

3 of 14

605837

autosomal recessive intellectual developmental disorder 38

NEK9; NIMA related kinase 9

3 of 14

609798

RCBTB1; RCC1 and BTB domain containing protein 1

3 of 14

607867

RETINAL DYSTROPHY WITH OR WITHOUT EXTRAOCULAR ANOMALIES; RDEOA

RCBTB2; RCC1 and BTB domain containing protein 2

3 of 14

603524

RCC2; regulator of chromosome condensation 2

3 of 14

609587

RCCD1; RCC1 domain containing 1

3 of 14

617997

RPGR; retinitis pigmentosa GTPase regulator

3 of 14

312610

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Rcc1 - HPS1

experimental knowledge based

(Guruharsha et al., 2011)

Rcc1 - dachs

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Rcc1

enhanceable

Mi-2

(Nikalayevich and Ohkura, 2015)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

hid

suppressible

Rcc1

(Shi and Skeath, 2004)

Mi-2

enhanceable

Rcc1

(Nikalayevich and Ohkura, 2015)

RanGAP

suppressible

Rcc1

(Wu et al., 2019, Kusano et al., 2001)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

64129

DroID - A comprehensive database of gene and protein interactions.

FBgn0002638

MIST (genetic) - An integrated Molecular Interaction Database

38669

MIST (protein-protein) - An integrated Molecular Interaction Database

38669

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Postmitotic nuclear pore complex (NPC) reformation

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-16

Cytogenetic map

65A1-65A1

Sequence location

3L:5,930,110..5,932,773 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

65A1-65A1

Limits computationally determined from genome sequence between P{PZ}vn¹⁰⁵⁶⁷ and P{PZ}loj⁰⁴⁰²⁶

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

64F-64F

(Eggert et al., 1998)

64F-64F

(determined by in situ hybridisation)

(Frasch, 1991)

Experimentally Determined Recombination Data

Location

3-16

(FlyBase, 2016)

3-14.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (12)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

37719

w¹¹¹⁸; P{UAS-Rcc1.S}3

16706

y¹ w^67c23; P{EPgy2}Rcc1^EY06262

36067

y¹ sc^* v¹ sev²¹; P{TRiP.GL00485}attP40

600198

w^*; Rcc1^C226 Fit2⁸³ P{FRT(w^hs)}2A/TM6B, P{Dfd-EYFP}3, Sb¹ Tb¹ ca¹

600257

w^*; P{Rcc1.GFP}M4/CyO; Rcc1^A47 Fit2⁸³ P{FRT(w^hs)}2A/TM6B, P{Dfd-EYFP}3, Sb¹ Tb¹ ca¹

37720

Rcc1^FF32 hry¹ Diap1^th-1 st¹ cu¹ sr¹ e^s/TM3, P{ftz-lacZ.ry⁺}TM3, Sb¹ ry^*

Kyoto

123439

w¹¹¹⁸; P{RS3}Rcc1^CB-5068-3

104713

w^*; P{GawB}Rcc1^NP4610 / TM3, Sb¹ Ser¹

NIG-Fly

10480R-1

P{NIG.10480R}1

HMJ21881

y¹ v¹; P{TRiP.HMJ21881}attP40/CyO

VDRC

v110321

P{KK100628}VIE-260B

v38389

w¹¹¹⁸; P{GD6989}v38389

Genomic Clones (23)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (205)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD22520

Other clones

DPiM_LD22520

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0002638

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0002638

Antibody Information

Laboratory Generated Antibodies

monoclonal

(Frasch, 1991)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Bj1 regulates nucleocytoplasmic transport and neural differentiation.

(Shi and Skeath, 2004)

Alleles of Bj1 show general defects in lateral CNS development.

(Shi and Skeath, 2002)

Mutants show supernumerary pigment cells between ommatidia, evident in the adult as a rough eye due to a disruption of the normally precise ommatidial pattern. This phenotype results from a failure of programmed cell death in the retinal epithelium.

(Gorski et al., 2000)

The maternally expressed Bj1 gene product binds to chromatin to establish or maintain a proper higher order structure as a prerequisite for regulated gene expression. Disruption of the structure may cause both misexpression and default repression of genes.

(Frasch, 1991)

Relationship to Other Genes

Source for database merge of

Source for merge of: Bj1 inxs

(Freeman et al., 2004)

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Bj1 CG10480

(Gabrielian, 1999.11)

Source for identity of: Bj1 side-out

(Shi, 2001.3.12)

Source for identity of: Rcc1 Bj1

(FlyBase, 2009-)

Nomenclature comments

Renamed from 'Bj1' to 'Rcc1' because: i) 'Bj1' is not informative - appears to refer to a antigen code (FBrf0053867); ii) 'Rcc1' was used in the original publication (FBrf0053867) and is popular in recent publications; iii) 'RCC1' is the HGNC symbol for the human ortholog.

(FlyBase, 2009-)

Etymology

Named "Rcc1" after the human ortholog.

(FlyBase, 2009-)

Synonyms and Secondary IDs (18)

Reported As

Symbol Synonym

BJ1

(Eggert et al., 2004, Eggert et al., 1999, Eggert et al., 1998, Bassett et al., 1997, Dasso, 1993, Foe et al., 1993)

Bj1

(Gingell and McLean, 2020, Bivik et al., 2015, Liang et al., 2014, Cui et al., 2013, Kwon et al., 2013, Kwon et al., 2013, Antao et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Neumüller et al., 2011, Hofmann et al., 2010, Mason and Goldfarb, 2009, Doheny et al., 2008, Kwon et al., 2008, Bakal et al., 2007)

CG10480

(Neuert et al., 2017, Kumar et al., 2016, Hosono et al., 2015, Cui et al., 2013, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Mason and Goldfarb, 2009)

CG18640 (Bj1)

(Kraut et al., 2001)

RCC1

(Zykova et al., 2021, Kasbekar, 2020, Nikalayevich and Ohkura, 2015, Poulton et al., 2014, Moutinho-Pereira et al., 2013, Cesario and McKim, 2011, Makde et al., 2010, Kwon et al., 2008, McElroy et al., 2008, Jang et al., 2007, Trieselmann and Wilde, 2002)

RanGEF

(Zhang et al., 2015, Kwon et al., 2008, McElroy et al., 2008, McLean et al., 2008, Johnson, 2002, Kusano et al., 2002, Hawley, 2001, Kusano et al., 2001, Kusano et al., 2001)

Rcc1

(Harkness et al., 2025, Yheskel et al., 2023, Kim et al., 2021, Park et al., 2020, Wu et al., 2019, Neuert et al., 2017, Solis et al., 2017, Boeynaems et al., 2016, Bivik et al., 2015, Colombié et al., 2013, Silverman-Gavrila and Wilde, 2006)

bj1

(Hampoelz et al., 2019)

dRCC1

(Solomon et al., 2018, Mason and Goldfarb, 2009)

inxs

(Gorski et al., 2000)

sdt

(Shi and Skeath, 2001)

Name Synonyms

Bj1 protein

CG18640 (Bj1), RCC protein

(Kraut et al., 2001)

Regulator of chromosome condensation 1

Regulator of chromosome condensation 1 ortholog

in excess

(Gorski et al., 2000)

regulator of chromosome condensation 1

(Moutinho-Pereira et al., 2013)

side-out

(Shi and Skeath, 2001)

Secondary FlyBase IDs

FBgn0041146

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 47 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38669

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P25171

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P25171

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P25171

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0002638

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38669/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0002638

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0002638

FlyMine - An integrated database for Drosophila genomics

FBgn0002638

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG10480

MARRVEL_MODEL - MARRVEL (model organism gene)

38669

PDB - An information portal to biological macromolecular structures

3MVD

Linkouts

BioGRID - A database of protein and genetic interactions.

64129

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0002638

DroID - A comprehensive database of gene and protein interactions.

FBgn0002638

DRSC - Results frm RNAi screens

FBgn0002638

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Rcc1_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0002638

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0002638

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10480

Flygut - An atlas of the Drosophila adult midgut

FBgn0002638

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0002638

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC031060

MIST (genetic) - An integrated Molecular Interaction Database

38669

MIST (protein-protein) - An integrated Molecular Interaction Database

38669

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Postmitotic nuclear pore complex (NPC) reformation

References (110)

The following external sites may use different assemblies or annotations than FlyBase.

0.78

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