FlyBase Gene Report: Dmel\ninaA

General Information

Symbol

Dmel\ninaA

Species

D. melanogaster

Name

neither inactivation nor afterpotential A

Annotation Symbol

CG3966

Feature Type

protein_coding_gene

FlyBase ID

FBgn0002936

Gene Model Status

Current

Stock Availability

16 publicly available

Enzyme Name (EC)

peptidylprolyl isomerase (5.2.1.8)

Gene Summary

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts on the folding of rhodopsin RH1 and RH2 (but not RH3) and is required for visual transduction. (UniProt, P15425)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

21E2-21E2

Sequence location

2L:877,302..878,270 [+]

Recombination map

2-1.5

RefSeq locus

NT_033779 REGION:877302..878270

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (12 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables cyclosporin A binding

inferred from mutant phenotype

(Shieh et al., 1989)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables cyclosporin A binding

inferred from biological aspect of ancestor with PANTHER:PTN008511653

(GO Reference Genome Project, 2011-)

enables peptidyl-prolyl cis-trans isomerase activity

traceable author statement

(Zuker, 1996, Colley et al., 1991)

inferred from biological aspect of ancestor with PANTHER:PTN008511653

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR002130, InterPro:IPR020892

(InterPro Project Members, 2004-)

Biological Process (5 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress

inferred from mutant phenotype

(Mendes et al., 2009)

involved_in N-glycan processing

inferred from mutant phenotype

(Rosenbaum et al., 2014)

involved_in rhodopsin biosynthetic process

inferred from mutant phenotype

(Rosenbaum et al., 2014, Ozaki et al., 1993)

inferred from genetic interaction with FLYBASE:Cnx99A; FB:FBgn0015622,FLYBASE:Xport-A; FB:FBgn0038749

(Rosenbaum et al., 2011)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in 'de novo' protein folding

traceable author statement

(Colley et al., 1991)

involved_in protein folding

inferred from electronic annotation with InterPro:IPR020892

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN008511653

(GO Reference Genome Project, 2011-)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

located_in cytoplasmic vesicle

inferred from direct assay

(Colley et al., 1991)

located_in endoplasmic reticulum lumen

inferred from direct assay

(Colley et al., 1991)

located_in endoplasmic reticulum membrane

inferred from direct assay

(Rosenbaum et al., 2011)

located_in membrane

inferred from direct assay

(Stamnes et al., 1991)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN008511653

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

CYCLOPHILINS

Protein Family (UniProt)

Belongs to the cyclophilin-type PPIase family. (P15425)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

peptidyl-prolyl cis-trans isomerase activity

[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0) (5.2.1.8)

RHEA 16237:

Summaries

Gene Group (FlyBase)

CYCLOPHILINS -: Cyclophilins are a class of evolutionary conserved peptidyl prolyl cis/trans isomerases. In vertebrates, they form binary complexes with their ligand, cyclosporine A, and are involved in key biochemical processes including protein folding, receptor signalling, protein trafficking, and transcription. Cyclophilins are characterized by the presence of a 'Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain' (IPR002130). (Adapted from PMID:25986569.)

Protein Function (UniProtKB)

(UniProt, P15425)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

ninaA: neither inactivation nor afterpotential A (J.C. Hall; B.H. Shieh)

Strong blue light leads to anomalously small degree of photoreceptor inactivation, and the prolonged depolarizing afterpotential PDA (seen after such treatment of wild-type photoreceptor cells) degrades rapidly following blue-light exposure reaching baseline levels within a few seconds; only the six outer photoreceptors (R1-R6) in each eye facet are aberrant physiologically (Larrivee et al., 1981). Rhodopsin levels are much lower than normal in these outer cells; altered gene dosage of ninaA+ does not effect changes in rhodopsin levels; ninaA's mutant phenotypes are not suppressed by feeding on retinoid (e.g. vitamin A-enriched) media.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\ninaA for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P15425)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.45

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

ninaA-RA

FBtr0078023

NM_057308

871

237

Additional Transcript Data and Comments

Reported size (kB)

0.95 (northern blot)

(Schneuwly et al., 1989)

0.9 (northern blot)

(Shieh et al., 1989)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

ninaA-PA

FBpp0077688

26.4

237

7.08

P15425

NP_476656

AAF51437

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

237 (aa)

(Stamnes et al., 1991, Shieh et al., 1989)

237 (aa); 26 (kD predicted)

(Schneuwly et al., 1989)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ninaA using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

ninaA_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.58

Transcript Expression

expression microarray

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

eye

(Xu et al., 2004)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 13 -- 16

Bolwig organ

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

pharate adult stage P8 -- pharate adult stage P15(i)

(Schneuwly et al., 1989)

adult stage

adult head

(Schneuwly et al., 1989)

Additional Descriptive Data

Eye-enriched transcripts determined by ratio of expression level in wild-type heads. versus expression level in so heads.

(Xu et al., 2004)

The ninaA transcript appears late in pupal development. It is present in adult heads, but is absent from adult bodies, and from heads of eya adults.

(Schneuwly et al., 1989)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

(Tian et al., 2013)

Additional Descriptive Data

ninaA protein is an integral membrane protein.

(Stamnes et al., 1991)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasmic vesicle

inferred from direct assay

(Colley et al., 1991)

located_in endoplasmic reticulum lumen

inferred from direct assay

(Colley et al., 1991)

located_in endoplasmic reticulum membrane

inferred from direct assay

(Rosenbaum et al., 2011)

located_in membrane

inferred from direct assay

(Stamnes et al., 1991)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\ninaA in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\ninaA

View exonic expression by tissue for Dmel\ninaA

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0002936

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

33271/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0002936

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0002936

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0002936

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3966

Flygut - An atlas of the Drosophila adult midgut

FBgn0002936

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 56 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of ninaA

Transgenic constructs containing regulatory region of ninaA

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

Df(2L)ast4

(Schneuwly et al., 1989)

Df(2L)Exel6003

(Ryder, 2004.4.26)

Gene not disrupted in

Df(2L)ast6

(Schneuwly et al., 1989)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

ninaA^GD795

viable, with Scer\GAL4^Mef2.PR

ninaA^GD795

viable, with Scer\GAL4^tin.CΔ4

ninaA^GD795

Other Phenotypes

Allele

abnormal neurophysiology

abnormal neurophysiology | adult stage | somatic clone

ninaA¹

abnormal touch response | larval stage, with Scer\GAL4^ChAT.PU

ninaA^RNAi.UAS.cUa

increased cell death

ninaA^E110V

visible | recessive | cold sensitive

ninaA¹²

visible | recessive | heat sensitive

ninaA²⁷

wild-type

ninaA^+t2.4

Phenotype manifest in

Allele

endoplasmic reticulum

ninaA³

endoplasmic reticulum | adult stage

ninaA³

ommatidium

ninaA^E110V

photoreceptor cell & endoplasmic reticulum

ninaA¹

photoreceptor cell R1

ninaA³

photoreceptor cell R2

ninaA³

photoreceptor cell R3

ninaA³

photoreceptor cell R4

ninaA³

photoreceptor cell R5

ninaA³

photoreceptor cell R6

ninaA³

photoreceptor neuron

retina | conditional

ninaA³

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (26)

Hsap\PPIB

5 of 14

Yes

Hsap\PPIC

5 of 14

Yes

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (20)

Rnor\Ppib

5 of 14

Yes

Rnor\Ppic

5 of 14

Yes

4 of 14

4 of 14

4 of 14

4 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Yes

Rnor\Ppil2

1 of 14

Rnor\Ppil6

1 of 14

Rnor\Ranbp2

1 of 14

Mus musculus (laboratory mouse) (17)

Mmus\Ppib

5 of 14

Yes

Mmus\Ppic

5 of 14

Yes

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (21)

Xtro\ppic

4 of 13

Yes

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\ppwd1

1 of 13

Danio rerio (Zebrafish) (17)

Drer\ppib

5 of 14

Yes

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (21)

Cele\cyn-5

5 of 14

Yes

Cele\cyn-6

5 of 14

Yes

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (13)

Agam\AgaP_AGAP009991

12 of 12

Yes

4 of 12

4 of 12

4 of 12

3 of 12

3 of 12

3 of 12

3 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (32)

Atha\AT4G34960

5 of 13

Yes

Atha\CYP5

5 of 13

Yes

Atha\ROC7

5 of 13

Yes

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

3 of 13

3 of 13

Yes

Atha\AT2G38730

3 of 13

Atha\AT3G22920

3 of 13

Atha\AT1G74070

2 of 13

Yes

Atha\AT4G32420

2 of 13

Atha\AT4G33060

2 of 13

Atha\AT5G35100

2 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\AT3G66654

1 of 13

Atha\ATE1

1 of 13

Atha\CYP38

1 of 13

Yes

Atha\CYP71

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (9)

Scer\CPR2

4 of 13

Yes

Scer\CPR5

4 of 13

Yes

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (8)

Spom\cyp4

4 of 12

Yes

3 of 12

3 of 12

3 of 12

2 of 12

2 of 12

2 of 12

1 of 12

Escherichia coli (enterobacterium) (2)

Ecol\ppiA

5 of 11

Yes

Ecol\ppiB

5 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:ninaA. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (18)

7 of 13

7 of 13

7 of 13

6 of 13

6 of 13

6 of 13

6 of 13

5 of 13

4 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

PPIB; peptidylprolyl isomerase B

model of osteogenesis imperfecta type 9

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PPIB; peptidylprolyl isomerase B

5 of 14

123841

OSTEOGENESIS IMPERFECTA, TYPE IX; OI9

osteogenesis imperfecta type 9

PPIC; peptidylprolyl isomerase C

5 of 14

123842

NKTR; natural killer cell triggering receptor

4 of 14

161565

PPIA; peptidylprolyl isomerase A

4 of 14

123840

PPIAL4A; peptidylprolyl isomerase A like 4A

4 of 14

PPIAL4C; peptidylprolyl isomerase A like 4C

4 of 14

PPIAL4D; peptidylprolyl isomerase A like 4D

4 of 14

PPIAL4E; peptidylprolyl isomerase A like 4E

4 of 14

608608

PPIAL4F; peptidylprolyl isomerase A like 4F

4 of 14

PPIAL4G; peptidylprolyl isomerase A like 4G

4 of 14

PPIAL4H; peptidylprolyl isomerase A like 4H

4 of 14

PPID; peptidylprolyl isomerase D

4 of 14

601753

PPIF; peptidylprolyl isomerase F

4 of 14

604486

PPIG; peptidylprolyl isomerase G

4 of 14

606093

PPIL1; peptidylprolyl isomerase like 1

4 of 14

601301

PONTOCEREBELLAR HYPOPLASIA, TYPE 14; PCH14

pontocerebellar hypoplasia type 14

PPIH; peptidylprolyl isomerase H

3 of 14

606095

PPIL3; peptidylprolyl isomerase like 3

3 of 14

615811

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

ninaA - Vps4

experimental knowledge based

(Guruharsha et al., 2011)

ninaA - ninaE

pull down, western blot

(Baker et al., 1994)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

ninaA

suppressible

ninaE

(Colley et al., 1991)

ninaA

enhanceable

PAPLA1

(Kunduri et al., 2014)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Apc

suppressible

ninaA

(Mendes et al., 2009)

Dcp-1

suppressible

ninaA

(Mendes et al., 2009)

p53

suppressible

ninaA

(Mendes et al., 2009)

rpr

suppressible

ninaA

(Mendes et al., 2009)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

59525

DroID - A comprehensive database of gene and protein interactions.

FBgn0002936

MIST (protein-protein) - An integrated Molecular Interaction Database

33271

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-1.5

Cytogenetic map

21E2-21E2

Sequence location

2L:877,302..878,270 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

21E2-21E2

Limits computationally determined from genome sequence between P{lacW}l(2)k09610^k09610&P{lacW}S^k09538a and P{PZ}l(2)10685¹⁰⁶⁸⁵&P{lacW}Tango14^k00619

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

21E1-21E1

(determined by in situ hybridisation)

(Schneuwly et al., 1989, Shieh et al., 1989)

Experimentally Determined Recombination Data

Location

2-1.5

(FlyBase, 2016)

2-0.7

(Comeron et al., 2012)

2-1.4

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (16)

Aberrations including deletions of this gene Aberrations including duplications of this gene

3533

64820

64821

3530

42261

64822

w^*; ninaA³; P{Rh1-ninaA}36

605627

y¹ w¹¹¹⁸; P{ninaE-ninaA.EGFP}2/CyO

NIG-Fly

3966R-2

P{NIG.3966R}2

3966R-3

P{NIG.3966R}3

M2L-2192

y¹ w¹¹¹⁸; ninaA^SK3 P{neoFRT}40A/CyO-Tb

VDRC

v51855

w¹¹¹⁸; P{GD795}v51855

v51856

w¹¹¹⁸; P{GD795}v51856

More stocks available...

Genomic Clones (11)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (7)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH08867

Other clones

DPiM_GH08867

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0002936

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

GH18403

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0002936

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Stamnes et al., 1991)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.

(Kliman and Eyre-Walker, 1998)

ninaA and ninaE bind to form a complex in vivo and the stability of the complex is dependent on the last six amino acids of the ninaA protein.

(Baker et al., 1994)

700,000 mutagenised flies were screened for visible deep pseudopupil ninaA phenotype, and 70 new alleles were isolated and characterised. Deep pseudopupil phenotype was scored on a 1-5 scale where 1= null ninaA phenotype and 5= weakest detectable ninaA phenotype. The rate of base changes suggests that mutagenic saturation of ninaA is being approached and that the majority of amino acid changes that remain unrecovered as mutations may be of no phenotypic consequence.

(Baker et al., 1994)

Major opsin genes can be transcribed in the absence of carotenoid, or retinoid. Expression of mature opsin is extremely depressed by carotenoid deprivation. The chromophore 11-cis-3-hydroxyretinal accelerates the synthesis of opsin by inducing its maturation.

(Ozaki et al., 1993)

(Lindsley and Zimm, 1992)

Mutations in ninaA severely inhibit opsin transport from the ER, leading to dramatic accumulations of ER cisternae in the photoreceptor cells.

(Colley et al., 1991)

ninaA gene product is an integral membrane protein. Ecol\lacZ reporter gene constructs demonstrate that ninaA is a tissue specific cyclophilin.

(Stamnes et al., 1991)

ninaA gene product is required for the synthesis of rhodopsin in a subset of photoreceptor cell types.

(Stamnes et al., 1991)

Only the six outer photoreceptors (R1-R6) in each eye facet are aberrant physiologically.Rhodopsin levels are much lower than normal in these outer cells; altered gene dosage of ninaA⁺ does not effect changes in rhodopsin levels; ninaA's mutant phenotypes are not suppressed by feeding on retinoid (e.g. vitamin A-enriched) media.

(Larrivee et al., 1981)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: ninaA CG3966

(Li, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

CG3966

(National Institute of Genetics Fly Stocks, 2022-, Park et al., 2014, Goldman and Arbeitman, 2007)

NINAA

(Tian et al., 2013, Pak et al., 2012)

NinaA

(Schopf and Huber, 2017, Roman-Fernandez and Bryant, 2016, Xu et al., 2016, Kunduri et al., 2014, Rosenbaum et al., 2014, Satoh et al., 2013, Xiong and Bellen, 2013, Rosenbaum et al., 2011, Satoh et al., 2010, Mendes et al., 2009, de Bruyne and Warr, 2006)

ninA

(Kliman and Eyre-Walker, 1998)

Name Synonyms

Neither inactivation nor after potential

(Kunduri et al., 2014)

either inactivation nor afterpotential A

(Goldman and Arbeitman, 2007)

neither inactivation nor afterpotential A

(Mendes et al., 2009, Lai et al., 2007)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 38 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

33271

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P15425

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P15425

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q540W6

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P15425

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0002936

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

33271/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0002936

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0002936

FlyMine - An integrated database for Drosophila genomics

FBgn0002936

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG3966

MARRVEL_MODEL - MARRVEL (model organism gene)

33271

Linkouts

BioGRID - A database of protein and genetic interactions.

59525

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0002936

DroID - A comprehensive database of gene and protein interactions.

FBgn0002936

DRSC - Results frm RNAi screens

FBgn0002936

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

ninaA_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0002936

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0002936

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3966

Flygut - An atlas of the Drosophila adult midgut

FBgn0002936

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC033125

MIST (protein-protein) - An integrated Molecular Interaction Database

33271

References (123)

The following external sites may use different assemblies or annotations than FlyBase.

-0.58

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