FlyBase Gene Report: Dmel\Nlp

General Information

Symbol

Dmel\Nlp

Species

D. melanogaster

Name

Nucleoplasmin

Annotation Symbol

CG7917

Feature Type

protein_coding_gene

FlyBase ID

FBgn0016685

Gene Model Status

Current

Stock Availability

10 publicly available

Gene Summary

Binds to core histones and functions in the ATP-facilitated assembly of approximately regularly spaced nucleosomal arrays. May participate in parallel with other histone-binding proteins such as NAP-1. (UniProt, Q27415)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
UniProtKB
Interactive Fly

Also Known As

dNLP, CRP1

Key Links

Genomic Location

Cytogenetic map

99D1-99D1

Sequence location

3R:30,004,785..30,010,352 [+]

Recombination map

3-100

RefSeq locus

NT_033777 REGION:30004785..30010352

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (10 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables histone binding

inferred from direct assay

(Emelyanov et al., 2014)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables chromatin binding

inferred from biological aspect of ancestor with PANTHER:PTN001100765

(GO Reference Genome Project, 2011-)

enables histone binding

inferred from biological aspect of ancestor with PANTHER:PTN001100765

(GO Reference Genome Project, 2011-)

traceable author statement

(Ito et al., 1997)

enables RNA binding

inferred from biological aspect of ancestor with PANTHER:PTN001100765

(GO Reference Genome Project, 2011-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in chromatin remodeling

inferred from direct assay

(Emelyanov et al., 2014)

involved_in sperm DNA decondensation

inferred from direct assay

(Emelyanov et al., 2014)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in chromatin remodeling

inferred from biological aspect of ancestor with PANTHER:PTN001100765

(GO Reference Genome Project, 2011-)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Emelyanov et al., 2014)

located_in nucleolus

inferred from direct assay

(Colflesh et al., 1999)

located_in nucleoplasm

inferred from high throughput direct assay

(Lye et al., 2014)

located_in nucleus

inferred from direct assay

(Emelyanov et al., 2014, Rohrbaugh et al., 2013, Colflesh et al., 1999, Ito et al., 1996)

colocalizes_with polytene chromosome interband

inferred from direct assay

(Rohrbaugh et al., 2013)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN001100765

(GO Reference Genome Project, 2011-)

is_active_in nucleolus

inferred from biological aspect of ancestor with PANTHER:PTN001100765

(GO Reference Genome Project, 2011-)

is_active_in nucleoplasm

inferred from biological aspect of ancestor with PANTHER:PTN001100765

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Protein Family (UniProt)

Belongs to the nucleoplasmin family. (Q27415)

Protein Signatures (InterPro)

Summaries

Protein Function (UniProtKB)

(UniProt, Q27415)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Nlp for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q27415)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

1NLQ

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Nlp-RA

FBtr0085552

NM_079833

1080

152

Nlp-RB

FBtr0334701

NM_001276165

914

152

Additional Transcript Data and Comments

Reported size (kB)

1.2 (northern blot)

(Ito et al., 1996)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Nlp-PA

FBpp0084918

17.0

152

4.24

FBpp0306756

17.0

152

4.24

Q27415

NP_001263094

AGB96473

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

152 aa isoforms: Nlp-PA, Nlp-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

152 (aa); 16.977 (kD predicted)

(Crevel et al., 1997)

152, 121 (aa); 22 (kD observed); 16.985, 15.522 (kD predicted)

(Ito et al., 1996)

Comments

In in vitro assays, purified native or recombinant Nlp protein coimmunoprecipitates with core histones. In a reaction containing DNA, purified core histones, ATP and partially purified CAF1 fraction, the longer Nlp protein was able to promote assembly of approximately regularly spaced nucleosome arrays, while the shorter Nlp protein could not. When Nap1 and Nlp proteins were compared, only Nap1 was able to promote sperm decondensation in Xenopus laevis sperm chromatin (contrast this with the

results of FBrf0093082).

(Crevel et al., 1997)

The shorter Nlp protein lacks 31 N-terminal residues found in the longer protein; this deletion abolishes the specificity of interaction with core histones.

(Ito et al., 1996)

External Data

Subunit Structure (UniProtKB)

Decamer formed by two pentameric rings associated in a head-to-head fashion.

(UniProt, Q27415)

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

1NLQ

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Nlp using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Nlp_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.26

Transcript Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Crevel et al., 1997)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Ito et al., 1996)

Additional Descriptive Data

Nlp and Nap1 proteins are expressed at highest levels in early embryos, and at reduced but significant levels in larval through adult stages.

(Ito et al., 1996)

Nlp protein localizes to nuclei of Schneider cells and of Drosophila embryos.

(Ito et al., 1996)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Emelyanov et al., 2014)

located_in nucleolus

inferred from direct assay

(Colflesh et al., 1999)

located_in nucleoplasm

inferred from high throughput direct assay

(Lye et al., 2014)

located_in nucleus

inferred from direct assay

(Emelyanov et al., 2014, Rohrbaugh et al., 2013, Colflesh et al., 1999, Ito et al., 1996)

colocalizes_with polytene chromosome interband

inferred from direct assay

(Rohrbaugh et al., 2013)

Expression Deduced from Reporters

Reporter: PBac{768.FSVS-0}Nlp^CPTI003532

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult gnathal ganglion

Comment: punctate expression throughout neuropil areas ( adult subesophageal ganglion, supraesophageal ganglion )

(Knowles-Barley, 2011.8.24)

adult brain cell body rind | restricted

Comment: subset of cells in the adult brain cortex

(Knowles-Barley, 2011.8.24)

adult brain cell body rind

Comment: adult brain cortex, higher around central brain, subset around optic lobe

(Knowles-Barley, 2011.8.24)

supraesophageal ganglion

Comment: punctate expression throughout neuropil areas ( adult subesophageal ganglion, supraesophageal ganglion )

(Knowles-Barley, 2011.8.24)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Nlp in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Nlp

View exonic expression by tissue for Dmel\Nlp

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0016685

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

43560/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0016685

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0016685

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0016685

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7917

Flygut - An atlas of the Drosophila adult midgut

FBgn0016685

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 6 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Nlp

Transgenic constructs containing regulatory region of Nlp

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(3R)Exel6213

(Ryder, 2004.4.26)

Gene partially disrupted in

Df(3R)Nlp-Nph

(Emelyanov et al., 2014)

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

Nlp^CC01224

viable, with Scer\GAL4^Mef2.PR

Nlp^GD12319

viable, with Scer\GAL4^pnr-MD237

Nlp^GD12319

viable, with Scer\GAL4^tin.CΔ4

Nlp^GD12319

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (3)

Hsap\NPM3

10 of 14

Yes

Hsap\NPM2

9 of 14

Yes

Hsap\NPM1

6 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (4)

Rnor\Npm2

10 of 14

Yes

Rnor\Npm3

9 of 14

Yes

Rnor\Npm1

7 of 14

Rnor\LOC300303

1 of 14

Yes

Mus musculus (laboratory mouse) (3)

Mmus\Npm2

9 of 14

Yes

Mmus\Npm3

9 of 14

Yes

Mmus\Npm1

6 of 14

Xenopus tropicalis (Western clawed frog) (5)

Xtro\npm3

8 of 13

Yes

Xtro\npm1

6 of 13

Yes

Xtro\npm2

5 of 13

Yes

Xtro\csf1

1 of 13

Xtro\XB5867546

1 of 13

Yes

Danio rerio (Zebrafish) (5)

Drer\npm2a

9 of 14

Yes

Drer\npm1b

8 of 14

Yes

Drer\npm2b

8 of 14

Yes

Drer\npm1a

6 of 14

Drer\npm3

6 of 14

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP004395

12 of 12

Yes

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Nlp. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (2)

Nph

9 of 13

CG43902

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

Nlp^HMS00556

model of primary ovarian insufficiency

CEA

(Gorsi et al., 2022)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Nlp^GD12319

ameliorates C9orf72 frontotemporal dementia and/or amyotrophic lateral sclerosis

modeled by Zzzz\poly-GR^50.UAS.EGFP

(Lee et al., 2016)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

NPM3; nucleophosmin/nucleoplasmin 3

10 of 14

606456

NPM2; nucleophosmin/nucleoplasmin 2

9 of 14

608073

NPM1; nucleophosmin 1

6 of 14

164040

LEUKEMIA, ACUTE MYELOID; AML

acute myeloid leukemia

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Nlp

comigration in sds page, molecular weight estimation by staining, protein cross-linking with a bifunctional reagent, electron microscopy, anti tag coimmunoprecipitation, anti tag western blot

(Anselm et al., 2018, Crevel et al., 1997)

Nlp - 14-3-3ε

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - ATPsynβ

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Acaa

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Akap200

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Aldo

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - AnxB10

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Ar1

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Ar2

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Bacc

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Boh1

anti tag coimmunoprecipitation, Identification by mass spectrometry, anti bait coimmunoprecipitation

(Lukacs et al., 2021)

Nlp - Boh2

anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation, Identification by mass spectrometry

(Lukacs et al., 2021)

Nlp - CG10265

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - CG11267

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - CG13983

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - CG14715

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - CG1910

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - CG2852

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - CG4598

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - CG5174

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - CG8993

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - CTCF

anti bait coimmunoprecipitation, western blot

(Padeken et al., 2013)

Nlp - Calr

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Chd64

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Cyp1

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Df31

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Dim1

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - ERp60

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Eb1

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - EndoGI

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Eno

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Fie

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Fim

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Fkbp12

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Fkbp14

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - GstE6

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - GstO3

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Gtpx

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - HINT1

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - His2A

cosedimentation, molecular weight estimation by staining, anti bait coimmunoprecipitation, cosedimentation through density gradient

(Emelyanov et al., 2014, Ito et al., 1996)

Nlp - His2B

cosedimentation, molecular weight estimation by staining, cosedimentation through density gradient, anti bait coimmunoprecipitation

(Emelyanov et al., 2014, Ito et al., 1996)

Nlp - His3

cosedimentation through density gradient, molecular weight estimation by staining, anti bait coimmunoprecipitation, cosedimentation

(Emelyanov et al., 2014, Ito et al., 1996)

Nlp - His4

cosedimentation through density gradient, molecular weight estimation by staining, cosedimentation, anti bait coimmunoprecipitation

(Emelyanov et al., 2014, Ito et al., 1996)

Nlp - HmgD

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Hmr

anti bait coimmunoprecipitation, Identification by mass spectrometry, anti tag coimmunoprecipitation, anti tag western blot

(Lukacs et al., 2021, Anselm et al., 2018)

Nlp - Hsp83

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Inos

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Lhr

anti bait coimmunoprecipitation, Identification by mass spectrometry

(Lukacs et al., 2021)

Nlp - Mdh2

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Moe

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Mst77F

anti bait coimmunoprecipitation, Identification by mass spectrometry, molecular weight estimation by staining

(Emelyanov et al., 2023)

Nlp - Nacα

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Nph

two hybrid, anti tag coimmunoprecipitation, anti tag western blot, pull down, molecular sieving, western blot, anti bait coimmunoprecipitation, Identification by mass spectrometry, experimental knowledge based

(Lukacs et al., 2021, Anselm et al., 2018, Hu et al., 2017.6.13, Guruharsha et al., 2011)

Nlp - Ntf-2

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - PCNA

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Pdi

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Pgam1

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Pgd

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - ProtA

anti tag coimmunoprecipitation, peptide massfingerprinting, pull down, anti tag western blot

(Emelyanov and Fyodorov, 2016, Emelyanov et al., 2014)

Nlp - Prx6c

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - RhoGDI

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Sh3β

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Sod1

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Stip1

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Su(var)205

anti bait coimmunoprecipitation, Identification by mass spectrometry

(Lukacs et al., 2021)

Nlp - Sumo

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - TER94

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Tango2

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Tctp

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Tkt

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - TpnC25D

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Trx2

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Trxr1

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - Uch

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - awd

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - cPges

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - capt

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - chic

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - cib

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - cid

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation

(Padeken et al., 2013)

Nlp - dUTPase

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - dhd

pull down, anti tag western blot

(Emelyanov and Fyodorov, 2016)

Nlp - dod

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - eEF1δ

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - eEF2

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - eEF5

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - fabp

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - fax

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - mod

anti bait coimmunoprecipitation, western blot

(Padeken et al., 2013)

Nlp - regucalcin

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - scf

experimental knowledge based

(Guruharsha et al., 2011)

Nlp - scu

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

Decamer formed by two pentameric rings associated in a head-to-head fashion.

(UniProt, Q27415 )

Linkouts

BioGRID - A database of protein and genetic interactions.

68416

DroID - A comprehensive database of gene and protein interactions.

FBgn0016685

MIST (protein-protein) - An integrated Molecular Interaction Database

43560

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-100

Cytogenetic map

99D1-99D1

Sequence location

3R:30,004,785..30,010,352 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

99D1-99D1

Limits computationally determined from genome sequence between P{PZ}ncd⁰⁵⁸⁸⁴ and P{lacW}l(3)s2500^s2500

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

99A-99A

(determined by in situ hybridisation)

(Crevel et al., 1997)

Experimentally Determined Recombination Data

Location

3-100

(FlyBase, 2016)

3-97.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (10)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

6976

w¹¹¹⁸; P{EP}Nlp^EP3555

51551

y¹ w^*; P{PTT-GC}Nlp^CC01224

15919

y¹ w^67c23; P{EPgy2}Nlp^EY03900

33688

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00556}attP2

97722

y¹ w^*; TI{CRIMIC.TG4.1}CG7912^{CR02987-TG4.1} Nlp^{CR02987-TG4.1-X}/TM3, Sb¹ Ser¹

Kyoto

115418

w¹¹¹⁸; PBac{768.FSVS-0}Nlp^CPTI003532

NIG-Fly

7917R-3

P{NIG.7917R}3

HMS00556

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00556}attP2

VDRC

v22623

w¹¹¹⁸; P{GD12319}v22623

v22625

w¹¹¹⁸; P{GD12319}v22625

Genomic Clones (14)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (109)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD16456

Other clones

DPiM_LD16456

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0016685

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0016685

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Ito et al., 1996)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Nlp protein can substitute for Xenopus nucleoplasmin in catalysing the in vitro decondensation of demembranated Xenopus sperm, this is accompanied by binding of Nlp to sperm chromatin.

(Crevel et al., 1997)

Nlp is a nuclear protein present throughout development. Purified and recombinant proteins bind to core histones and can function in the assembly of approximately regularly spaced nucleosomal arrays.

(Ito et al., 1996)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

CG7917

(Çiçek et al., 2016, Cammarato et al., 2011, Ni et al., 2010.12.1)

CRP1

(Colflesh et al., 1999, Crevel et al., 1997, Ito et al., 1997, Crevel, 1996.7.11)

Crp1

NLP

(Emelyanov et al., 2023, Lukacs et al., 2021, Okimune et al., 2020, Anselm et al., 2018, Emelyanov and Fyodorov, 2016, Boija and Mannervik, 2015, Emelyanov et al., 2014, Padeken et al., 2013, Thomae et al., 2013)

Nlp

(Choudhury et al., 2024, Titlow et al., 2023, Gorsi et al., 2022, Michki et al., 2021, Meltzer et al., 2019, Gene Disruption Project members, 2018-, Hu et al., 2017.6.13, Lee et al., 2016, Noble et al., 2016, Dent et al., 2015, Kwon et al., 2013, Rohrbaugh et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Friedman et al., 2011, Singer and Gall, 2011, Nie et al., 2009, McElroy et al., 2008, Buszczak et al., 2007)

dCAF-4

(Ito et al., 1996)

dNLP

(Khuong et al., 2017, Fei et al., 2015, Rohrbaugh et al., 2013, Ellis, 2006, Namboodiri et al., 2003, Ridgway and Almouzni, 2001, Tyler et al., 1999, Crevel et al., 1997, Ito et al., 1997, Ito et al., 1997, Ito, 1996.5.30, Ito et al., 1996)

dNLP-S

(Ito, 1996.5.30)

nlp

(Joyce et al., 2012)

p22

(Ito et al., 1997)

Name Synonyms

Chromatin decondensation protein 1

Nucleoplasmin

(Ogienko et al., 2024, Rohrbaugh et al., 2013, Cammarato et al., 2011, Singer and Gall, 2011)

Nucleoplasmin-like

(Ito et al., 1996)

nucleoplasmin

(Lukacs et al., 2021)

nucleoplasmin-1

(Mello and Almouzni, 2001)

Secondary FlyBase IDs

FBgn0016133

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 48 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

43560

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q27415

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q27415

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

A0A0B4KI24

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q27415

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0016685

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

43560/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0016685

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0016685

FlyMine - An integrated database for Drosophila genomics

FBgn0016685

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG7917

MARRVEL_MODEL - MARRVEL (model organism gene)

43560

PDB - An information portal to biological macromolecular structures

1NLQ

Linkouts

BioGRID - A database of protein and genetic interactions.

68416

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0016685

DroID - A comprehensive database of gene and protein interactions.

FBgn0016685

DRSC - Results frm RNAi screens

FBgn0016685

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Nlp_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0016685

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0016685

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7917

Flygut - An atlas of the Drosophila adult midgut

FBgn0016685

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0016685

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC012176

MIST (protein-protein) - An integrated Molecular Interaction Database

43560

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (82)

The following external sites may use different assemblies or annotations than FlyBase.

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