FlyBase Gene Report: Dmel\ebd1

larval somatic muscle cell

(Fisher et al., 2012)

larval nervous system

(Fisher et al., 2012)

(Fisher et al., 2012)

gonad

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

adult stage

adult mushroom body | restricted

(Kobayashi et al., 2006)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- larval stage

embryonic/larval salivary gland

(Benchabane et al., 2011)

(Benchabane et al., 2011)

(Benchabane et al., 2011)

(Benchabane et al., 2011)

(Benchabane et al., 2011)

(Benchabane et al., 2011)

embryonic stage 16

(Benchabane et al., 2011)

(Benchabane et al., 2011)

(Benchabane et al., 2011)

(Benchabane et al., 2011)

(Benchabane et al., 2011)

larval stage

third instar larval stage

(Benchabane et al., 2011)

myoblast of wing disc

(Benchabane et al., 2011)

(Benchabane et al., 2011)

larval brain

(Benchabane et al., 2011)

photoreceptor neuron

(Benchabane et al., 2011)

embryonic/larval salivary gland

(Benchabane et al., 2011)

(Benchabane et al., 2011)

wing disc

(Benchabane et al., 2011)

(Benchabane et al., 2011)

pupal stage

(Benchabane et al., 2011)

adult salivary gland

(Benchabane et al., 2011)

pupal stage P6

(Benchabane et al., 2011)

Additional Descriptive Data

ebd1 protein is expressed in embryos in neurons, glia, muscle cells, and garland cells. In larvae and pupae, expression persists in the same cell types and also in salivary glands. In pupae, it stains the dorsal medial muscle fibres and myoblasts. Expression is observed in third instar larvae in wing disc-associated myoblasts, neurons

(Benchabane et al., 2011)

and glial cells and also in photoreceptor cells and larval brain.

(Benchabane et al., 2011)

ebd1 is expressed in neurons, glial cells, myoblasts, myofibres, and nephrocytes in embryos. Expression persists in the same cell types in larvae and pupae and it is also present in salivary glands. Expression is observed in dorsal medial muscles (also known as the DLMs). It is also expressed in the brain, in wing_disc-associated myoblasts and in photoreceptor cells in third instar larvae.

(Benchabane et al., 2011)

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

Reporter: P{ebd1-Gal4.B}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 16

(Benchabane et al., 2011)

(Benchabane et al., 2011)

(Benchabane et al., 2011)

(Benchabane et al., 2011)

(Benchabane et al., 2011)

larval stage

(Benchabane et al., 2011)

(Benchabane et al., 2011)

(Benchabane et al., 2011)

embryonic/larval salivary gland

(Benchabane et al., 2011)

(Benchabane et al., 2011)

pupal stage

adult salivary gland

(Benchabane et al., 2011)

pupal stage P6

View Dmel\ebd1 in JBrowse

(Benchabane et al., 2011)

Reporter: P{GawB}ebd1^NP3004

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

enterocyte

(Tian et al., 2017)

enteroendocrine cell

(Tian et al., 2017)

enteroblast

(Tian et al., 2017)

intestinal stem cell

(Tian et al., 2017)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\ebd1

View exonic expression by tissue for Dmel\ebd1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0035153

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38082/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0035153

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0035153

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0035153

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3371

Flygut - An atlas of the Drosophila adult midgut

FBgn0035153

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\ebd1

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 7 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of ebd1

Transgenic constructs containing regulatory region of ebd1

Aberrations (Deficiencies and Duplications) ( 9 )

Inferred from experimentation ( 9 )

Gene disrupted in

Df(3L)27-3

(Benchabane et al., 2011)

Df(3L)ED4136

(Benchabane et al., 2011)

Df(3L)ED4157

(Benchabane et al., 2011)

Df(3L)ED4196

(Benchabane et al., 2011)

Df(3L)ED9698

(Benchabane et al., 2011)

Df(3L)Exel6085

(Ryder, 2004.4.26)

Gene partially disrupted in

Df(3L)5

(Benchabane et al., 2011)

Gene not disrupted in

Df(3L)ED4125

(Benchabane et al., 2011)

Df(3L)ED4238

(Benchabane et al., 2011)

Inferred from location ( 9 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

ebd1^GD10952

viable, with Scer\GAL4^pnr-MD237

ebd1^GD10952

Other Phenotypes

Allele

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav-C155

ebd1^EP3592

increased cell number, with Scer\GAL4^NP0001

increased cell number | adult stage

ebd1²⁴⁰

increased cell number | adult stage | cell non-autonomous | somatic clone

increased cell number | adult stage | somatic clone

ebd1²⁴⁰

increased occurrence of cell division, with Scer\GAL4^NP0001

increased occurrence of cell division | cell non-autonomous | somatic clone

Phenotype manifest in

Allele

adult midgut epithelium

ebd1²⁴⁰

adult midgut epithelium, with Scer\GAL4^NP0001

adult midgut epithelium | cell non-autonomous

anterior fascicle & synapse, with Scer\GAL4^elav-C155

ebd1^EP3592