FlyBase Gene Report: Dmel\garz

General Information

Symbol

Dmel\garz

Species

D. melanogaster

Name

gartenzwerg

Annotation Symbol

CG8487

Feature Type

protein_coding_gene

FlyBase ID

FBgn0264560

Gene Model Status

Current

Stock Availability

12 publicly available

Gene Summary

gartenzwerg (garz) encodes a guanine nucleotide exchange factor localized to the cis-Golgi apparatus. The roles of the product of garz include membrane and protein trafficking between the Golgi apparatus and the endoplasmic reticulum. It is required for epithelial morphogenesis in the embryo. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Red Book
Interactive Fly

Also Known As

ang, CG 8487, sec7-family GEF

Key Links

Genomic Location

Cytogenetic map

48F8-48F10

Sequence location

2R:12,326,360..12,333,860 [+]

Recombination map

2-66

RefSeq locus

NT_033778 REGION:12326360..12333860

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (15 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables guanyl-nucleotide exchange factor activity

inferred from genetic interaction with FLYBASE:Arf1; FB:FBgn0010348,FLYBASE:ArfGAP1; FB:FBgn0020655

(Armbruster and Luschnig, 2012)

inferred from genetic interaction with FLYBASE:Arf1; FB:FBgn0010348

(Khadilkar et al., 2014)

enables small GTPase binding

inferred from physical interaction with UniProtKB:O18332

(Sechi et al., 2017)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables guanyl-nucleotide exchange factor activity

inferred from electronic annotation with InterPro:IPR000904, InterPro:IPR035999

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN008950430

(GO Reference Genome Project, 2011-)

Biological Process (11 terms)

Terms Based on Experimental Evidence (9 terms)

CV Term

Evidence

References

involved_in ARF protein signal transduction

inferred from mutant phenotype

(Armbruster and Luschnig, 2012)

involved_in epithelial cell development

inferred from direct assay

(Szul et al., 2011)

involved_in Golgi organization

inferred from mutant phenotype

(Armbruster and Luschnig, 2012)

involved_in lumen formation, open tracheal system

inferred from direct assay

(Wang et al., 2012)

involved_in positive regulation of endocytosis

inferred from genetic interaction with FLYBASE:Arf1; FB:FBgn0010348

(Khadilkar et al., 2014)

involved_in protein secretion

inferred from mutant phenotype

(Armbruster and Luschnig, 2012)

involved_in regulation of tube diameter, open tracheal system

inferred from mutant phenotype

(Armbruster and Luschnig, 2012)

involved_in salivary gland development

inferred from direct assay

(Szul et al., 2011)

involved_in secretion by cell

inferred from direct assay

(Wang et al., 2012)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in Golgi organization

inferred from biological aspect of ancestor with PANTHER:PTN008503207

(GO Reference Genome Project, 2011-)

involved_in regulation of ARF protein signal transduction

inferred from electronic annotation with InterPro:IPR000904, InterPro:IPR035999

(InterPro Project Members, 2004-)

involved_in retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum

inferred from biological aspect of ancestor with PANTHER:PTN008503207

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in cis-Golgi network

inferred from direct assay

(Wang et al., 2012)

located_in Golgi cis cisterna

inferred from direct assay

(Armbruster and Luschnig, 2012)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in Golgi cis cisterna

inferred from biological aspect of ancestor with PANTHER:PTN008503207

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

ARF GUANINE NUCLEOTIDE EXCHANGE FACTORS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

ARF GUANINE NUCLEOTIDE EXCHANGE FACTORS -: ArfGEFs are Guanine Nucleotide Exchange Factors (GEFs) for ARF family GTPases. They are characterized by a central Sec7 catalytic domain domain with homology to yeast SEC7. (Adapted from PMID:15731001, FBrf0158741 and FBrf0220529).

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

ang: angle wing

Wings held up from dorsal surfaces and extended outward 15-90 from the mid-dorsal line. Longitudinal dorsal median muscles 5 and 6 fused (Goldberg, 1954, Ph.D. Thesis, Ill. Inst. Technol.). No increase in expressivity with temperature. Does not overlap wild type. RK2.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\garz for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A1Z8W8)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.49

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

garz-RA

FBtr0087898

NM_165881

5631

1740

garz-RB

FBtr0087897

NM_136917

6630

1983

garz-RC

FBtr0305075

NM_001259349

5634

1741

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

garz-PA

FBpp0087011

194.9

1740

5.66

FBpp0087010

220.6

1983

5.08

FBpp0293612

194.9

1741

5.66

A0A0B4K7N0

NP_001246278

AFH08032

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\garz using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

garz_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.77

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

embryonic stage 10 -- 11

embryonic/larval hindgut

(Wang et al., 2012)

embryonic stage 10 -- 16

embryonic/larval salivary gland

(Wang et al., 2012)

embryonic stage 10 -- 17

embryonic/larval proventriculus

(Wang et al., 2012)

larval tracheal system

(Wang et al., 2012)

embryonic stage 11 -- 12

organism | faint | ubiquitous

(Fisher et al., 2012)

salivary gland body primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic/larval salivary gland

(Fisher et al., 2012)

embryonic/larval salivary gland body

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Isoform-specific expression patterns are observed. Sex-specific expression of alternative transcripts observed.

(Telonis-Scott et al., 2009)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cis-Golgi network

inferred from direct assay

(Wang et al., 2012)

located_in Golgi cis cisterna

inferred from direct assay

(Armbruster and Luschnig, 2012)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\garz in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\garz

View exonic expression by tissue for Dmel\garz

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0264560

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36337/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0264560

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0033714

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0264560

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8487

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 15 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of garz

Transgenic constructs containing regulatory region of garz

Aberrations (Deficiencies and Duplications) ( 7 )

Inferred from experimentation ( 7 )

Gene disrupted in

Df(2R)Exel6061

(Armbruster and Luschnig, 2012, Ryder, 2004.4.26)

Gene not disrupted in

Df(2L)ed1

(Reuter and Szidonya, 1983)

Df(2L)ed2

(Reuter and Szidonya, 1983)

Df(2L)M24F11

(Reuter and Szidonya, 1983)

Df(2L)M24F-B

(Reuter and Szidonya, 1983)

Df(2L)tkv2

(Reuter and Szidonya, 1983)

Df(2L)tkv3

(Reuter and Szidonya, 1983)

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Mef2.PR

garz^GD13940

lethal, with Scer\GAL4^pnr-MD237

garz^GD13940

lethal (with garz^EMS667)

garz^S4-50

lethal (with garz^EP2028)

garz^S4-50

lethal (with garz^S4-50)

lethal | pupal stage, with Scer\GAL4^pnr-MD237

garz^GD13940

lethal - all die before end of first instar larval stage

lethal - all die before end of first instar larval stage (with Df(2R)Exel6061)

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

garz^GD13940

viable, with Scer\GAL4^tin.CΔ4

garz^GD13940

Other Phenotypes

Allele

abnormal developmental rate

garz^Δ137

abnormal developmental rate (with Df(2R)Exel6061)

garz^Δ137

abnormal developmental rate | embryonic stage (with Df(2R)Exel6061)

garz^S4-50

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav-C155

garz^EP2028

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^elav-C155

garz^JF01603

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^RapGAP1-OK6

garz^JF01603

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

garz^GD13940

some die during first instar larval stage

some die during first instar larval stage (with Df(2R)Exel6061)

visible, with Scer\GAL4^GMR.PF

visible | recessive

garz¹

Phenotype manifest in

Allele

anterior fascicle & synapse, with Scer\GAL4^elav-C155

garz^EP2028

anterior fascicle & synapse | supernumerary, with Scer\GAL4^elav-C155

garz^EP2028

cis-Golgi network | embryonic stage

garz^Δ137

cortical zone of lymph gland primary lobe | third instar larval stage, with Scer\GAL4^Hml.Δ

garz^RNAi.UAS

dorsal longitudinal indirect flight muscle cell

garz¹

embryonic/first instar larval cuticle

garz^Δ137

embryonic/larval crystal cell | third instar larval stage, with Scer\GAL4^Hml.Δ

garz^RNAi.UAS

embryonic/larval cuticle (with Df(2R)Exel6061)

garz^R1044

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^{SPARC-MI00329-GAL4}

garz^HMS01397

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^elav-C155

garz^JF01603

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^RapGAP1-OK6

garz^JF01603

embryonic/larval plasmatocyte | third instar larval stage, with Scer\GAL4^Hml.Δ

garz^RNAi.UAS

embryonic/larval salivary gland, with Scer\GAL4^fkh.PH

garz^EP2028

embryonic/larval salivary gland (with Df(2R)Exel6061)

garz^R1044

embryonic/larval tracheal section

garz^Δ137

extracellular matrix | third instar larval stage, with Scer\GAL4^{SPARC-MI00329-GAL4}

garz^HMS01397

eye, with Scer\GAL4^GMR.PF

eye photoreceptor cell, with Scer\GAL4^GMR.PF

garz^EP2028

hemocyte | third instar larval stage, with Scer\GAL4^srp.PU

garz^GD13940

larval intersegmental nerve, with Scer\GAL4^elav-C155

garz^EP2028

NMJ bouton | third instar larval stage, with Scer\GAL4^elav-C155

garz^JF01603

NMJ bouton | third instar larval stage, with Scer\GAL4^RapGAP1-OK6

garz^JF01603

ommatidium | adult stage, with Scer\GAL4^ninaE.PU

garz^UAS.cUa

ommatidium | adult stage | progressive | conditional, with Scer\GAL4^ninaE.PU

garz^GD13940

posterior signaling center of lymph gland primary lobe | third instar larval stage, with Scer\GAL4^Hml.Δ

garz^RNAi.UAS

retina | adult stage | progressive | conditional, with Scer\GAL4^ninaE.PU

garz^GD13940

rhabdomere, with Scer\GAL4^GMR.PF

garz^EP2028

rough endoplasmic reticulum | embryonic stage

garz^Δ137

salivary gland | embryonic stage

garz^Δ137

secondary lobe of embryonic/larval lymph gland | third instar larval stage, with Scer\GAL4^Hml.Δ

garz^RNAi.UAS

tracheal lumen

garz^R1044

tracheal lumen, with Scer\GAL4^btl.PS

tracheal lumen (with Df(2R)Exel6061)

garz^R1044

tracheal lumen | embryonic stage

garz^Δ137

vesicle | embryonic stage

garz^Δ137

wing

garz¹

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (17)

Hsap\GBF1

14 of 14

Yes

4 of 14

4 of 14

3 of 14

3 of 14

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (16)

Rnor\Gbf1

14 of 14

Yes

4 of 14

4 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (17)

Mmus\Gbf1

14 of 14

Yes

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (12)

Xtro\gbf1

11 of 13

Yes

Xtro\fbxo8

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (22)

Drer\gbf1

14 of 14

Yes

4 of 14

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (5)

Cele\gbf-1

12 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (7)

Agam\AgaP_AGAP001527

10 of 12

Yes

3 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (9)

Atha\GN

13 of 13

Yes

Atha\GNL1

12 of 13

Yes

Atha\GNL2

12 of 13

Yes

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (5)

Scer\GEA1

12 of 13

Yes

Scer\GEA2

12 of 13

Yes

Scer\SYT1

3 of 13

Yes

Scer\SEC7

2 of 13

Scer\YEL1

1 of 13

Schizosaccharomyces pombe (Fission yeast) (6)

Spom\SPBC211.03c

10 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:garz. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (6)

5 of 13

5 of 13

4 of 13

4 of 13

2 of 13

2 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

GBF1; golgi brefeldin A resistant guanine nucleotide exchange factor 1

model of Charcot-Marie-Tooth disease dominant intermediate A

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

garz^EP2028

exacerbates tauopathy

modeled by Hsap\MAPT^V337M.UAS

(Shulman and Feany, 2003)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

GBF1; golgi brefeldin A resistant guanine nucleotide exchange factor 1

14 of 14

603698

CHARCOT-MARIE-TOOTH DISEASE, AXONAL, TYPE 2GG; CMT2GG

Charcot-Marie-Tooth disease dominant intermediate A

ARFGEF1; ARF guanine nucleotide exchange factor 1

4 of 14

604141

DEVELOPMENTAL DELAY, IMPAIRED SPEECH, AND BEHAVIORAL ABNORMALITIES, WITH OR WITHOUT SEIZURES; DEDISB

ARFGEF2; ARF guanine nucleotide exchange factor 2

4 of 14

605371

PERIVENTRICULAR HETEROTOPIA WITH MICROCEPHALY, AUTOSOMAL RECESSIVE; ARPHM

periventricular nodular heterotopia

3 of 14

3 of 14

FBXO8; F-box protein 8

3 of 14

605649

IQSEC1; IQ motif and Sec7 domain ArfGEF 1

3 of 14

IQ MOTIF- AND SEC7 DOMAIN-CONTAINING PROTEIN 1; IQSEC1

INTELLECTUAL DEVELOPMENTAL DISORDER WITH SHORT STATURE AND BEHAVIORAL ABNORMALITIES; IDDSSBA

intellectual developmental disorder with short stature and behavioral abnormalities

IQSEC3; IQ motif and Sec7 domain ArfGEF 3

3 of 14

612118

PSD2; pleckstrin and Sec7 domain containing 2

3 of 14

620656

PSD3; pleckstrin and Sec7 domain containing 3

3 of 14

614440

PSD4; pleckstrin and Sec7 domain containing 4

3 of 14

614442

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Dmel gene

Ortholog showing functional complementation

Supporting References

garz

Hsap\GBF1

(Armbruster and Luschnig, 2012)

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

garz - Arf1

pull down, peptide massfingerprinting

(Christis and Munro, 2012)

garz - Rab1

anti tag coimmunoprecipitation, western blot, pull down, peptide massfingerprinting

(Sechi et al., 2017, Gillingham et al., 2014)

garz - Rab6

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

garz

enhanceable

Arf1

(Armbruster and Luschnig, 2012)

garz

enhanceable

Cds

(Raghu et al., 2009)

garz

suppressible

Vps35

(Thakur et al., 2016)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Cds

enhanceable

garz

(Raghu et al., 2009)

Pld

suppressible

garz

(Thakur et al., 2016)

Pld

enhanceable

garz

(Raghu et al., 2009)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0264560

MIST (genetic) - An integrated Molecular Interaction Database

36337

MIST (protein-protein) - An integrated Molecular Interaction Database

36337

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Endocytosis

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-66

Cytogenetic map

48F8-48F10

Sequence location

2R:12,326,360..12,333,860 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

48F8-48F10

Limits computationally determined from genome sequence between P{EP}garz^EP2028 and P{lacW}l(2)k11404^k11404

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-66

(FlyBase, 2016)

2-68.3

(Comeron et al., 2012)

2-10.5

(Reuter and Szidonya, 1983)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (12)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

2389

garz¹

24324

w¹¹¹⁸; Mi{ET1}garz^MB05159

17017

w¹¹¹⁸; P{EP}garz^EP2028/CyO

31232

y¹ v¹; P{TRiP.JF01603}attP2

37664

y¹ w^*; Mi{MIC}garz^MI04410

34987

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01397}attP2/TM3, Sb¹

Kyoto

114444

y^d2 w¹¹¹⁸ P{ey-FLP.N}2; P{neoFRT}42D P{EP}garz^EP2028 /CyO y⁺

NIG-Fly

8487R-2

P{NIG.8487R}2

2RG-1227

y² VhaAC39-1^cho-1 v¹; P{U6-garz.NIG.sgRNA}attP40/CyO

HMS01397

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01397}attP2/TM3, Sb¹

VDRC

v42140

w¹¹¹⁸; P{GD13940}v42140

v42141

w¹¹¹⁸; P{GD13940}v42141/CyO

Genomic Clones (19)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (25)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

AT08349

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0264560

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Wang et al., 2012)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

garz is not involved in invagination or in the elongation of salivary gland cells, but is involved in apical membrane expansion.

(Wang et al., 2012)

garz depletion inhibits AP-1 recruitment to membranes.

(Szul et al., 2011)

S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

(Stroschein-Stevenson et al., 2006)

dsRNA made from templates generated with primers directed against garz that is transfected into S2 treated with Listeria monocytogenes reveals garz to be involved in Listeria monocytogenes entry.

(Cheng et al., 2005)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Identification: in a screen for genes which when overexpressed cause motorneuron pathfinding and or synaptogenesis errors.

(Kraut and Zinn, 2001)

Involved in endocytosis/membrane trafficking.

(Kraut and Zinn, 2001)

Relationship to Other Genes

Source for database merge of

Source for merge of: garz ang

(Armbruster and Luschnig, 2012)

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: garz CG8487

(Kraut, 2002.3.6)

Nomenclature comments

Etymology

The gene is named "angina" based on the narrow tracheal lumen phenotype seen in mutant embryos.

(Armbruster and Luschnig, 2012)

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

ARF1GEF

(Khadilkar et al., 2014)

ArfGEF

(Wang et al., 2012)

CG 8487

(Kraut et al., 2001, Kraut et al., 2001)

CG8487

(National Institute of Genetics Fly Stocks, 2022-, Hosono et al., 2015, Sing et al., 2014, Armbruster and Luschnig, 2012, Christis and Munro, 2012, Wang et al., 2012, Ni et al., 2011.7.19, Gupta et al., 2009, Ni et al., 2008, Cox et al., 2004, Shulman and Feany, 2003)

GBF1

(Cox et al., 2004)

GEF

(Khadilkar et al., 2014)

Garz

(Fujii et al., 2020, Sechi et al., 2017, Hemalatha et al., 2016, Sopko et al., 2015, Khadilkar et al., 2014, D'Ambrosio and Vale, 2010)

ang

(Armbruster and Luschnig, 2012)

Name Synonyms

Gartenzwerg

(Khadilkar et al., 2014, Szul et al., 2011, Kraut and Zinn, 2001)

angina

(Armbruster and Luschnig, 2012)

angle wing

gartenzwerg

(Khadilkar et al., 2014, Armbruster and Luschnig, 2012, Reid et al., 2012, Wang et al., 2012, Kühnlein, 2010, Gupta et al., 2009, Gabut et al., 2007, Kraut, 2002.3.6)

sec7-family GEF

(Kraut et al., 2001, Kraut et al., 2001)

Secondary FlyBase IDs

FBgn0033714
FBgn0063599
FBgn0000081

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 46 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

36337

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A1Z8W8

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A1Z8W8

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0264560

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36337/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0264560

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0264560

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG8487

MARRVEL_MODEL - MARRVEL (model organism gene)

36337

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0264560

DroID - A comprehensive database of gene and protein interactions.

FBgn0264560

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

garz_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0033714

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0264560

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8487

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0264560

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC005830

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Endocytosis

MIST (genetic) - An integrated Molecular Interaction Database

36337

MIST (protein-protein) - An integrated Molecular Interaction Database

36337

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (89)

The following external sites may use different assemblies or annotations than FlyBase.

1.77

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