Human Disease Model Report: cancer, epithelial, RAB5-microRNA-related

General Information

Name

cancer, epithelial, RAB5-microRNA-related

FlyBase ID

FBhh0001533

Disease Ontology Term

cancer

Parent Disease

OMIM

Overview

Assessed in larval eye discs, loss-of-function mutations of Dmel\Rab5 in clones of cells overexpressing a growth regulator microRNA, mir-ban (bantam), result in dramatic tumor growth and malignant invasion of adjacent tissues, specifically the ventral nerve cord. Neither overexpression of mir-ban alone nor Rab5 mutation alone causes tumor growth or metastatic behavior. This system has been used to assess transcriptome changes over the course of tumor growth and progression.

There are three human genes orthologous to Dmel\Rab5, RAB5A, RAB5B, and RAB5C. UAS constructs of tagged Hsap\RAB5C and Hsap\RAB5A human genes have been introduced into flies, but have not been characterized in the context of this disease model.

[updated Sep. 2023 by FlyBase; FBrf0222196]

Disease Summary Information

Disease Summary: cancer, epithelial, RAB5-microRNA-related

OMIM report

Human gene(s) implicated

Symptoms and phenotype

Genetics

Cellular phenotype and pathology

Molecular information

The Rab family of proteins is a member of the Ras superfamily of monomeric G proteins. Rab GTPases regulate many steps of membrane traffic, including vesicle formation, vesicle movement along actin and tubulin networks, and membrane fusion. [https://www.genenames.org/cgi-bin/genefamilies/set/388]

(FlyBase Curators, 2013-)

External links

COSMIC gene: RAB5C
COSMIC gene: RAB5A
COSMIC gene: RAB5B
Gene2Function (human gene): RAB5A
Gene2Function (human gene): RAB5B
Gene2Function (human gene): RAB5C
Gene Cards: RAB5A
Gene Cards: RAB5B
Gene Cards: RAB5C
MedlinePlus (gene): RAB5A
MedlinePlus (gene): RAB5B
MedlinePlus (gene): RAB5C
MARRVEL (human gene): RAB5A
MARRVEL (human gene): RAB5B
MARRVEL (human gene): RAB5C
NCBI (Entrez) gene: RAB5A; RAB5A, member RAS oncogene family
NCBI (Entrez) gene: RAB5B; RAB5B, member RAS oncogene family
NCBI (Entrez) gene: RAB5C; RAB5C, member RAS oncogene family

Disease synonyms

cancer, bantam-RAB5-related

Related Diseases

Related human health report(s)

cancer, intercellular interactions, RAB5-related

cancer, multiple, Hippo signaling pathway

Ortholog Information

Human gene(s) in FlyBase

Human gene (HGNC)

Symbol / Name

RAB5C; RAB5C, member RAS oncogene family

D. melanogaster ortholog (based on DIOPT)

Dmel\Rab5

(DIOPT, 2013-)

Comments on ortholog(s)

Many to one: 3 human to 1 Drosophila. The human genes are RAB5A, RAB5B, and RAB5C.

Human gene (HGNC)

Symbol / Name

RAB5A; RAB5A, member RAS oncogene family

D. melanogaster ortholog (based on DIOPT)

Dmel\Rab5

(DIOPT, 2013-)

Comments on ortholog(s)

Many to one: 3 human to 1 Drosophila. The human genes are RAB5A, RAB5B, and RAB5C.

Human gene (HGNC)

Symbol / Name

RAB5B; RAB5B, member RAS oncogene family

D. melanogaster ortholog (based on DIOPT)

Dmel\Rab5

(DIOPT, 2013-)

Comments on ortholog(s)

Many to one: 3 human to 1 Drosophila. The human genes are RAB5A, RAB5B, and RAB5C.

Other mammalian ortholog(s) used

D. melanogaster Gene Information (2)

Dmel\mir-ban

Gene Snapshot

Contributions welcome

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

Comments on ortholog(s)

5' seed sequence homologous to that of human MIR450B-3p (FBrf0242402).

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\Rab5

Gene Snapshot

Rab5 (Rab5) encodes a monomeric GTPase that controls entry of endocytosed cargo into the early endosome and is required for vesicle re-uptake at the synapse. Altering the activity of the product of Rab5 affects many receptor-mediated signaling pathways as well as epithelial polarity. [Date last reviewed: 2019-03-14]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

Comments on ortholog(s)

Moderate- to high-scoring ortholog of human RAB5A, RAB5B, and RAB5C (1 Drosophila to 3 human).

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Other Genes Used: Viral, Bacterial, Synthetic (0)

Summary of Physical Interactions (143 groups)

mir-ban

RNA-protein

Interacting group

Assay

References

mir-ban - AGO1

anti bait coimmunoprecipitation, northern blot, anti tag coimmunoprecipitation, nuclease footprinting, autoradiography, filter binding, competition binding, pull down, western blot

(Kakumani et al., 2020, Wen et al., 2015, Yang et al., 2014, Carré et al., 2013, Hur et al., 2013, Nishida et al., 2013, Eulalio et al., 2009, Flynt et al., 2009, Miyoshi et al., 2009, Eulalio et al., 2008, Zhou et al., 2008, Förstemann et al., 2007, Rehwinkel et al., 2006, Miyoshi et al., 2005, Okamura et al., 2004)

mir-ban - AGO2

anti tag coimmunoprecipitation, northern blot, filter binding, autoradiography

(Yang et al., 2014, Hur et al., 2013, Rehwinkel et al., 2006, Okamura et al., 2004)

mir-ban - bel

anti tag coimmunoprecipitation, northern blot

(Zhou et al., 2008)

mir-ban - Dcr-1

electrophoretic mobility shift assay, autoradiography, enzymatic study, anti tag coimmunoprecipitation, northern blot

(Liu et al., 2007, Ye et al., 2007, Saito et al., 2005)

mir-ban - drosha

enzymatic study, northern blot

(Denli et al., 2004)

mir-ban - Fmr1

anti bait coimmunoprecipitation, quantitative reverse transcription pcr, northern blot, anti tag coimmunoprecipitation

(Yang et al., 2009, Okamura et al., 2004)

mir-ban - gw

anti tag coimmunoprecipitation, northern blot, anti bait coimmunoprecipitation

(Eulalio et al., 2009, Eulalio et al., 2009, Miyoshi et al., 2009, Zekri et al., 2009)

mir-ban - His1

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Liu et al., 2016)

mir-ban - loqs

anti tag coimmunoprecipitation, northern blot, anti bait coimmunoprecipitation, fluorescence polarization spectroscopy, predetermined participant

(Tants et al., 2017, Miyoshi et al., 2009, Saito et al., 2005)

mir-ban - pasha

enzymatic study, northern blot

(Denli et al., 2004)

mir-ban - Ran

anti tag coimmunoprecipitation, northern blot

(Zhou et al., 2008)

mir-ban - ref(2)P

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Ghosh et al., 2024)

mir-ban - RpL21

anti tag coimmunoprecipitation, northern blot

(Zhou et al., 2008)

mir-ban - RpL22

anti tag coimmunoprecipitation, northern blot

(Zhou et al., 2008)

mir-ban - RpL32

anti tag coimmunoprecipitation, northern blot

(Zhou et al., 2008)

mir-ban - RpS7

anti tag coimmunoprecipitation, northern blot

(Zhou et al., 2008)

mir-ban - SmD1

anti bait coimmunoprecipitation, primer specific pcr

(Xiong et al., 2015)

mir-ban - Unr

pull down, western blot

(Kakumani et al., 2020)

mir-ban - vig

anti tag coimmunoprecipitation, northern blot

(Zhou et al., 2008)

RNA-RNA

Interacting group

Assay

References

mir-ban - cic

luminiscence technology, necessary binding region

(Herranz et al., 2012)

mir-ban - Clk

luminiscence technology, necessary binding region

(Kadener et al., 2009)

mir-ban - hid

fluorescence technology, immunohistochemistry, luminiscence technology, necessary binding region

(Shen et al., 2015, Kertesz et al., 2007, Leaman et al., 2005, Robins et al., 2005, Brennecke et al., 2003)

mir-ban - Mad

luminiscence technology, necessary binding region

(Robins et al., 2005)

mir-ban - mei-P26

luminiscence technology, necessary binding region, immunohistochemistry

(Herranz et al., 2010)

mir-ban - numb

luminiscence technology, necessary binding region

(Wu et al., 2017)

mir-ban - Tgi

luminiscence technology, necessary binding region, immunohistochemistry, western blot

(Shen et al., 2015)

mir-ban - trbl

fluorescence technology

(Gerlach et al., 2019)

Rab5

protein-protein

Interacting group

Assay

References

Rab5 - Agpat3

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - Arf1

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - Arl8

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - Atg6

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Rab5 - ATPsynγ

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - Bulli

pull down, peptide massfingerprinting

(Gillingham et al., 2014, Gillingham et al., 2014)

Rab5 - car

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Rab5 - cathD

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - CCT3

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - CCT6

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - Ccz1

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Rab5 - cDIP

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Rab5 - CG1416

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - CG1513

pull down, peptide massfingerprinting

(Gillingham et al., 2014, Gillingham et al., 2014)

Rab5 - CG3558

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Rab5 - CG4041

pull down, peptide massfingerprinting

(Gillingham et al., 2014, Gillingham et al., 2014)

Rab5 - CG6607

pull down, peptide massfingerprinting, anti tag western blot

(Gillingham et al., 2014, Gillingham et al., 2014)

Rab5 - CG9205

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Rab5 - CG9986

pull down, peptide massfingerprinting

(Gillingham et al., 2014, Gillingham et al., 2014)

Rab5 - CG15098

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - CG34126

pull down, peptide massfingerprinting

(Gillingham et al., 2014, Gillingham et al., 2014)

Rab5 - CG41099

pull down, peptide massfingerprinting

(Gillingham et al., 2014, Gillingham et al., 2014, Fabrowski et al., 2013)

Rab5 - Chc

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - Cnx99A

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - CtsL1

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - Cyp12d1-p

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - Dlic

anti tag coimmunoprecipitation, western blot, Identification by mass spectrometry

(Satoh et al., 2008)

Rab5 - dome

anti tag coimmunoprecipitation, western blot

(Chen et al., 2018)

Rab5 - dor

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Rab5 - Droj2

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - fax

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - fz

pull down, anti tag western blot

(Lin and Katanaev, 2013)

Rab5 - Gαo

pull down, anti tag western blot, western blot

(Purvanov et al., 2010)

Rab5 - Gdi

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based, pull down

(Gillingham et al., 2014, Rhee et al., 2014)

Rab5 - Gfat2

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - Gp93

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - Hap40

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Rab5 - Hel25E

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - hook

pull down, peptide massfingerprinting, western blot

(Gillingham et al., 2014, Gillingham et al., 2014, Gillingham et al., 2014)

Rab5 - htt

pull down, peptide massfingerprinting

(Gillingham et al., 2014, Gillingham et al., 2014)

Rab5 - Idh3a

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - Ipo9

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - KdelR

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - Kdsr

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - Khc-73

pull down, peptide massfingerprinting

(Gillingham et al., 2014, Gillingham et al., 2014)

Rab5 - Lam

pull down, western blot, affinity chromatography technology, Identification by mass spectrometry

(Capalbo et al., 2011)

Rab5 - Ldh

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - Lrrk

anti tag coimmunoprecipitation, anti tag western blot

(Dodson et al., 2014, Dodson et al., 2012)

Rab5 - Mad

anti tag coimmunoprecipitation, anti tag western blot

(Gui et al., 2016)

Rab5 - Mcad

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - Mec2

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - Mhcl

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Rab5 - Mon1

pull down, peptide massfingerprinting, protein three hybrid

(Hegedűs et al., 2016, Gillingham et al., 2014)

Rab5 - mop

anti tag coimmunoprecipitation, anti tag western blot

(Chen et al., 2012)

Rab5 - MRP

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - mt:CoII

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - mud

affinity chromatography technology, Identification by mass spectrometry, anti bait coimmunoprecipitation, anti tag western blot

(Capalbo et al., 2011)

Rab5 - mv

anti tag coimmunoprecipitation, western blot, Identification by mass spectrometry

(Lattao et al., 2021)

Rab5 - Nadsyn

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Rab5 - Nfs1

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - Ocrl

pull down, peptide massfingerprinting

(Gillingham et al., 2014, Gillingham et al., 2014)

Rab5 - Ost1

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - Ost48

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - OstΔ

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - ox

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - peo

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Rab5 - Pi3K59F

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Rab5 - Pi3K68D

anti tag coimmunoprecipitation, anti tag western blot

(Jean et al., 2012)

Rab5 - put

anti tag coimmunoprecipitation, anti tag western blot

(Gui et al., 2016)

Rab5 - Rab1

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - Rab2

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - Rab3-GAP

pull down, peptide massfingerprinting

(Gillingham et al., 2014, Gillingham et al., 2014)

Rab5 - Rab3GAP1

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Rab5 - Rab6

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - Rab7

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - Rabex-5

pull down, peptide massfingerprinting

(Gillingham et al., 2014, Gillingham et al., 2014)

Rab5 - Rap1

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - Rbcn-3A

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Rab5 - Rbcn-3B

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Rab5 - Rbpn-5

pull down, anti tag western blot, peptide massfingerprinting, western blot

(Gillingham et al., 2014, Gillingham et al., 2014, Thomas and Strutt, 2014)

Rab5 - Rbsn-5

pull down, anti tag western blot, peptide massfingerprinting, western blot, autoradiography

(Gillingham et al., 2014, Gillingham et al., 2014, Mottola et al., 2010, Morrison et al., 2008, Tanaka and Nakamura, 2008)

Rab5 - Rdh1

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - rogdi

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Rab5 - Rok

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Rab5 - RpS10b

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - Rpt1

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - sau

anti tag coimmunoprecipitation, western blot

(Sechi et al., 2014)

Rab5 - scrib

anti tag coimmunoprecipitation, anti tag western blot

(Gui et al., 2016)

Rab5 - ScsβA

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - ScsβG

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - Sec61α

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - SERCA

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - sp3

pull down, peptide massfingerprinting

(Gillingham et al., 2014, Gillingham et al., 2014)

Rab5 - spidey

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - spri

anti tag coimmunoprecipitation, anti tag western blot, pull down

(Rowshanravan et al., 2014, Sakuma et al., 2014)

Rab5 - Spt-I

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - SsRβ

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - Strip

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation

(Sakuma et al., 2014)

Rab5 - tacc

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Rab5 - Tapδ

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - Tbc1d15-17

pull down, anti tag western blot, peptide massfingerprinting

(Gillingham et al., 2014, Gillingham et al., 2014, Gillingham et al., 2014)

Rab5 - Tecr

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - tkv

anti tag coimmunoprecipitation, anti tag western blot

(Gui et al., 2016)

Rab5 - Tspo

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - Ubr1

pull down, peptide massfingerprinting

(Gillingham et al., 2014, Gillingham et al., 2014)

Rab5 - UQCR-C2

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - Uvrag

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Rab5 - Vha68-2

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - Vps8

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Rab5 - Vps15

pull down, peptide massfingerprinting

(Gillingham et al., 2014, Gillingham et al., 2014)

Rab5 - Vps16A

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Rab5 - Vps35

proximity ligation assay, fluorescence microscopy

(Inoshita et al., 2017)

Rab5 - Vps45

pull down, peptide massfingerprinting

(Gillingham et al., 2014, Gillingham et al., 2014)

Rab5 - wal

experimental knowledge based

(Guruharsha et al., 2011)

Rab5 - wts

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

Rab5 - ZnT63C

experimental knowledge based

(Guruharsha et al., 2011)

Alleles Reported to Model Human Disease (Disease Ontology) (22 alleles)

mir-ban

Models Based on Experimental Evidence ( 2 )

Allele

Disease

Evidence

References

mir-ban^C.UAS.EGFP

model of carcinoma

CEA with Egfr^UAS.cBa

(Herranz et al., 2012)

model of cancer

CEA with Rab5^LL00467

(Cong et al., 2021)

mir-ban^UAS.cUa

model of cancer

CEA with Egfr^UAS.cBa

(Eichenlaub et al., 2016)

Modifiers Based on Experimental Evidence ( 7 )

Allele

Disease

Interaction

References

mir-ban^{sponge.UAS.DsRed(Unk)}

ameliorates carcinoma

modeled by Ras85D^V12.UAS, l(2)gl⁴

(Ito and Igaki, 2021)

mir-ban^D.UAS.EGFP

ameliorates neurodegenerative disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Bilen et al., 2006)

mir-ban^B90.1

ameliorates neurodegenerative disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Bilen et al., 2006)

mir-ban^EP3208

exacerbates neurodegenerative disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Bilen et al., 2006)

mir-ban^C.UAS.EGFP

ameliorates neurodegenerative disease

modeled by Zzzz\CAG^{127Q.UAS.Tag:HA}

(Dubey and Tapadia, 2018)

mir-ban^Δ.as.B.UAS

exacerbates neurodegenerative disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Bilen et al., 2006)

mir-ban^EP3622

exacerbates neurodegenerative disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Bilen et al., 2006)

Rab5

Models Based on Experimental Evidence ( 5 )

Allele

Disease

Evidence

References

Rab5²

model of carcinoma

CEA

(Lu and Bilder, 2005)

model of cancer

CEA

(Sollazzo et al., 2018)

Rab5^S43N.UAS

model of cancer

CEA

(Yamamoto et al., 2017)

model of carcinoma

CEA with scrib¹

(Igaki et al., 2009)

Rab5^HMS00147

model of kidney disease

CEA

(Xi et al., 2024)

Rab5^GD10492

model of cancer

CEA

(Ballesteros-Arias et al., 2014)

Rab5^LL00467

model of cancer

CEA with mir-ban^C.UAS.EGFP

(Cong et al., 2021)

Modifiers Based on Experimental Evidence ( 13 )

Allele

Disease

Interaction

References

Rab5²

ameliorates amyotrophic lateral sclerosis type 8

modeled by Vap33^Δ20

(Mao et al., 2019)

ameliorates autosomal dominant adult-onset proximal spinal muscular atrophy

modeled by Vap33^Δ20

(Mao et al., 2019)

Rab5^EY10619

exacerbates viral infectious disease

modeled by EBV\BRLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

exacerbates infectious mononucleosis

modeled by EBV\BRLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

exacerbates Epstein-Barr virus hepatitis

modeled by EBV\BRLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

ameliorates amyotrophic lateral sclerosis type 8

modeled by Vap33^{P58S.UAS.Tag:FLAG}

(Sanhueza et al., 2015)

exacerbates amyotrophic lateral sclerosis type 10

modeled by Hsap\TARDBP^UAS.cUa

(Liu et al., 2017)

modeled by Hsap\TARDBP^G298S.UAS

(Liu et al., 2017)

Rab5^KG05684

exacerbates viral infectious disease

modeled by EBV\BZLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

exacerbates infectious mononucleosis

modeled by EBV\BZLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

exacerbates Epstein-Barr virus hepatitis

modeled by EBV\BZLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

Rab5^S43N.UAS

ameliorates tauopathy

modeled by Hsap\MAPT^GMR.Ex.PJ

(Bakhoum et al., 2014)

ameliorates epilepsy

modeled by zyd¹

(Weiss et al., 2019)

ameliorates brain cancer

modeled by Vps35^E42

(Li et al., 2018)

ameliorates Chediak-Higashi syndrome

modeled by mv³

(Lattao et al., 2021)

modeled by mv^ros

(Lattao et al., 2021)

Rab5^UAS.EGFP

exacerbates Huntington's disease

modeled by Hsap\HTT^GMR.Q120

(Ravikumar et al., 2008)

ameliorates amyotrophic lateral sclerosis type 15

modeled by Hsap\UBQLN2^WT.UAS

(Kim et al., 2023)

modeled by Hsap\UBQLN2^P4X.UAS

(Kim et al., 2023)

modeled by Hsap\UBQLN2^P497H.UAS

(Kim et al., 2023)

ameliorates Alzheimer's disease 1

modeled by Hsap\APP^2xAPP.SP.UAS

(Yu et al., 2020)

ameliorates Huntington's disease

modeled by Hsap\HTT^{N548.Q128.UAS}

(Martin et al., 2021)

Rab5^JF03335

exacerbates amyotrophic lateral sclerosis type 15

modeled by Hsap\UBQLN2^WT.UAS

(Kim et al., 2023)

modeled by Hsap\UBQLN2^P4X.UAS

(Kim et al., 2023)

modeled by Hsap\UBQLN2^P497H.UAS

(Kim et al., 2023)

Rab5^HMS00147

exacerbates amyotrophic lateral sclerosis type 15

modeled by Hsap\UBQLN2^WT.UAS

(Kim et al., 2023)

modeled by Hsap\UBQLN2^P4X.UAS

(Kim et al., 2023)

modeled by Hsap\UBQLN2^P497H.UAS

(Kim et al., 2023)

exacerbates amyotrophic lateral sclerosis type 8

modeled by Vap33^{P58S.UAS.Tag:FLAG}

(Sanhueza et al., 2015)

ameliorates epilepsy

modeled by zyd¹

(Weiss et al., 2019)

Rab5^GD10492

model of cancer

is ameliorated by Dronc^GD12376

(Ballesteros-Arias et al., 2014)

is ameliorated by puc^UAS.cMa

(Ballesteros-Arias et al., 2014)

Rab5^UASp.YFP

ameliorates motor neuron disease

modeled by scat^HMS01910

(Patel et al., 2020)

exacerbates amyotrophic lateral sclerosis type 10

modeled by Hsap\TARDBP^G298S.UAS

(Liu et al., 2017)

Rab5^{S43N.UASp.YFP}

ameliorates Alzheimer's disease

modeled by Hsap\APP^695.UAS

(Xu et al., 2016)

ameliorates motor neuron disease

modeled by scat^HMS01910

(Patel et al., 2020)

Rab5^{Q88L.UASp.YFP}

ameliorates epilepsy

modeled by para^bss1

(Kroll et al., 2015)

Rab5^KK102895

exacerbates Huntington's disease

modeled by Hsap\HTT^{N548.Q128.UAS}

(Martin et al., 2021)

Rab5^KO

ameliorates Alzheimer's disease 1

modeled by Appl^d

(Kessissoglou et al., 2020)

Alleles Representing Disease-Implicated Variants

Genetic Tools, Stocks and Reagents

Sources of Stocks

Contact lab of origin for a reagent not available from a public stock center.

Bloomington Stock Center Disease Page

Related mammalian, viral, bacterial, or synthetic transgenes

Allele

Transgene

Publicly Available Stocks

Hsap\RAB5C^UAS.Tag:HA

PBac{UAS-hRAB5C.HA}

y¹ w^*; PBac{UAS-hRAB5C.HA}VK00033

Hsap\RAB5A^UAS.Tag:HA

PBac{UAS-hRAB5A.HA}

y¹ w^*; PBac{UAS-hRAB5A.HA}VK00033/TM3, Sb¹ Ser¹

Selected Drosophila transgenes

Allele

Transgene

Publicly Available Stocks

Rab5^S43N.UAS

P{UAS-Rab5.S43N}

w^*; P{UAS-Rab5.S43N}2

Rab5^UAS.EGFP

P{UAS-GFP-Rab5}

w^*; P{UAS-GFP-Rab5}3

Rab5^UASp.YFP

P{UASp-YFP.Rab5}

y¹ w^*; P{UASp-YFP.Rab5}Pde8^08b

Rab5^{S43N.UASp.YFP}

P{UASp-YFP.Rab5.S43N}

y¹ w^*; P{UASp-YFP.Rab5.S43N}01

Rab5^{Q88L.UASp.YFP}

P{UASp-YFP.Rab5.Q88L}

w¹¹¹⁸; P{UASp-YFP.Rab5.Q88L}01a/TM3, Ser¹

mir-ban^UAS.cUa

P{UAS-mir-ban.U}

mir-ban^D.UAS.EGFP

P{UAS-EGFP-mir-ban.D}

mir-ban^C.UAS.EGFP

P{UAS-EGFP-mir-ban.C}

w^*; P{UAS-EGFP-mir-ban.C}3

mir-ban^Δ.as.B.UAS

P{UAS-mir-ban.Delta.as.B}

mir-ban^{sponge.UAS.DsRed(Unk)}

P{UAS-dsRed.mir-ban.sponge}

RNAi constructs available

Allele

Transgene

Publicly Available Stocks

Rab5^GD10492

P{GD10492}

w¹¹¹⁸ P{GD10492}v34094

y¹ v¹; P{TRiP.JF03335}attP2

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00147}attP2

y¹ v¹; P{TRiP.HMC03420}attP40

y¹ sc^* v¹ sev²¹; P{TRiP.GL01872}attP40

Selected Drosophila classical alleles

Allele

Allele class

Mutagen

Publicly Available Stocks

Rab5^KO

Rab5^KG05684

P-element activity

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{SUPor-P}Rab5^KG05684 P{neoFRT}40A/CyO y⁺

Rab5^EY10619

P-element activity

y^d2 w¹¹¹⁸ P{ey-FLP.N}2; P{EPgy2}Rab5^EY10619 P{neoFRT}40A/CyO y⁺

Rab5^LL00467

y^* w^*; PBac{SAstopDsRed}LL00467 P{neoFRT}40A P{FRT(w^hs)}G13 cn¹ bw¹/CyO, S^* bw¹

Rab5²

loss of function allele

P-element activity

P{hsFLP}1, y¹ w^*; Rab5² P{neoFRT}40A/In(2LR)Gla, wg^Gla-1 PPO1^Bc

P-element activity

P-element activity

Delta2-3 transposase

amorphic allele - genetic evidence

Delta2-3 transposase

References (5)

Report Sections

Open Close