Abstract
Humoral factors act as inter-tissue mediators and regulate various organismal physiologies. Although the importance of humoral factors in tissue repair has been recently recognized, our understanding of how humoral proteins regulate tissue repair remains limited. Glycosylation is an important modification of the conventional secretory pathway, and we have demonstrated that N-glycosylation in the Golgi apparatus of the Drosophila fat body, the major secretory tissue equivalent to the mammalian liver and adipose tissue, remotely contributes to epithelial tissue repair. To identify humoral factors that contribute to repair via the Golgi apparatus, we constructed a Golgi-specific protein biotinylation system and performed hemolymph proteomics. By combining genetic analyses, we found that fat body-derived innate immune regulators and iron mediators affect tissue repair. Altogether, our Golgi-specific labeling system has the potential to identify Golgi-mediated secreted factors that regulate inter-organ communication.
