FlyBase Allele Report: Dmel\dally[gem]

General Information

Symbol

Dmel\dally^gem

Species

D. melanogaster

Name

FlyBase ID

FBal0098753

Feature type

allele

Associated gene

Dmel\dally

Associated Insertion(s)

H{}dally^gem

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

loss of function allele

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Chorsky and Belote, 1994)

hypomorphic allele - genetic evidence

(Tsuda et al., 1999)

loss of function allele

(Kirkpatrick et al., 2006)

Mutagen

Progenitor genotype

Associated Insertion(s)

H{}dally^gem

Cytology

Description

Insertion of a hobo element into exon 4 of dally. The insertion leads to a frameshift generating two consecutive stop codons, leading to a truncated protein.

(Merino et al., 2022)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

anterior dorsocentral bristle

(Kirkpatrick et al., 2006)

anterior dorsocentral bristle (with dally⁰⁶⁴⁶⁴)

(Fujise et al., 2001)

anterior dorsocentral bristle (with dally^ΔP-188)

(Fujise et al., 2001)

anterior dorsocentral bristle (with dally^ΔP-527)

(Kirkpatrick et al., 2006, Fujise et al., 2001)

anterior dorsocentral bristle (with Df(3L)Scf-R6)

(Kirkpatrick et al., 2006)

anterior dorsocentral bristle | absent

(Nakato et al., 2024)

dorsal row of triple row of wing sensilla (with dally⁰⁶⁴⁶⁴)

(Fujise et al., 2001)

dorsocentral bristle | increased number, with Scer\GAL4^A9, dally^UAS.cJa

(Kirkpatrick et al., 2006)

dorsocentral bristle | increased number (with dally^ΔP-527), with Scer\GAL4^A9, dally^UAS.cJa

(Kirkpatrick et al., 2006)

embryonic epidermis (with dally^ΔP-188)

(Tsuda et al., 1999)

eye

(Nakato et al., 2024)

female germline stem cell

(Guo and Wang, 2009)

genital disc

(Chorsky and Belote, 1994)

genitalia (with dally^ΔP-188)

(Tsuda et al., 1999)

longitudinal vein

(Koh et al., 2023)

male genitalia | absent

(Koh et al., 2023)

posterior dorsocentral bristle (with dally⁰⁶⁴⁶⁴)

(Fujise et al., 2001)

posterior dorsocentral bristle (with dally^ΔP-188)

(Fujise et al., 2001)

posterior dorsocentral bristle (with dally^ΔP-527)

(Fujise et al., 2001)

posterior postalar bristle (with dally^ΔP-527)

(Luders et al., 2003)

posterior supraalar bristle

(Kirkpatrick et al., 2006)

posterior supraalar bristle (with dally⁰⁶⁴⁶⁴)

(Fujise et al., 2001)

posterior supraalar bristle (with dally^ΔP-188)

(Fujise et al., 2001)

posterior supraalar bristle (with dally^ΔP-527)

(Kirkpatrick et al., 2006, Fujise et al., 2001)

posterior supraalar bristle (with Df(3L)Scf-R6)

(Kirkpatrick et al., 2006)

sensory mother cell (with dally^ΔP-527)

(Fujise et al., 2001)

wing

(Akiyama et al., 2022)

wing (with dally^ΔP-527)

(Luders et al., 2003)

wing disc | larval stage

(Merino et al., 2022)

wing margin

(Koh et al., 2023, Kleinschmit et al., 2010)

wing margin bristle

(Kleinschmit et al., 2010)

wing margin bristle | anterior

(Kirkpatrick et al., 2006)

wing margin bristle | anterior (with dally^ΔP-527)

(Kirkpatrick et al., 2006)

wing margin bristle | anterior (with Df(3L)Scf-R6)

(Kirkpatrick et al., 2006)

wing margin bristle | decreased number

(Nakato et al., 2024)

wing nerve TSM(1) & sensillum campaniformium (with dally^ΔP-527)

(Fujise et al., 2001)

wing nerve TSM(2) & sensillum campaniformium (with dally^ΔP-527)

(Fujise et al., 2001)

wing sensillum

(Dejima et al., 2013)

wing vein L5

(Nakato et al., 2024, Akiyama et al., 2022, Kirkpatrick et al., 2006, Takeo et al., 2005, Chorsky and Belote, 1994)

wing vein L5 (with dally^ΔP-527)

(Kirkpatrick et al., 2006, Luders et al., 2003)

wing vein L5 (with Df(3L)Scf-R6)

(Kirkpatrick et al., 2006)

Detailed Description

Statement

Reference

dally^gem mutant flies exhibit a reduction in the number of chemosensory bristles. In dally^gem heterozygous mutants, the average number of chemosensory bristles is slightly lower than wild-type, but the difference is not statistically significant.

(Dejima et al., 2013)

dally^gem mutant animals display a much reduced number of chemosensory and mechanosensory bristles at the anterior dorsal wing margin compared to wild-type. No ectopic mechanosensory bristles are observed.

(Kleinschmit et al., 2010)

The ovarioles of homozygous females show progressive loss of the germline stem cells (GSCs), with 91% of ovarioles containing no GSCs in the niche at day 10 after eclosion. The number of cap cells in the niche is normal in the mutant ovarioles. Programmed cell death is not detected in cap cells or germline cells in the mutant germaria.

(Guo and Wang, 2009)

dally^gem flies show a reduction in the number of chemosensory bristles at the anterior margin of the wing compared to wild-type flies. The L5 vein in the wings of these flies does not form properly and stops short between the posterior crossvein and the wing margin. These mutants also show a loss of the anterior dorsocentral bristles and the posterior supraalar bristles on the notum. This phenotype is also seen in dally^gem/dally^ΔP-527 and dally^gem/Df(3L)Scf-R6 transheterozygotes.

Expression of dally^Scer\UAS.cJa under the control of Scer\GAL4^A9 in a dally^gem/dally^ΔP-527 or dally^gem mutant background results in the formation of excess dorsocentral bristles (the excess is around 30% compared to wild-type wings).

(Kirkpatrick et al., 2006)

Wing vein V does not reach the posterior edge of the wing in dally^gem mutants.

(Takeo et al., 2005)

In dally^gem/dally^ΔP-527 adults, the posterior postalar bristle (pPA) is absent from 5% of all individuals. These adults also display defects in wing patterning. In addition to notches in the margin, the wings of these mutants show a truncation of longitudinal vein L5.

(Luders et al., 2003)

The number of the multiply innervated (recurved) bristles in the dorsal row of dally⁰⁶⁴⁶⁴/dally^gem wings decreases to 55% of that of wild-type flies. dally^ΔP-527/dally^gem animals show a loss of both twin campaniform sensilla of the margin (TSM1 and 2) in 70% of animals. In dally^gem/dally^ΔP-527 flies, 98% of animals lack the posterior supraalar bristle (pSA), 93% the anterior dorsocentral bristles (aDC) and 3% the posterior supraalar bristle (pSA). In dally^gem/dally⁰⁶⁴⁶⁴ flies, 86% of animals lack the posterior supraalar bristle (pSA), 24% the anterior dorsocentral bristles (aDC) and 1% the posterior supraalar bristle (pSA). In dally^gem/dally^ΔP-188 flies, 96% of animals lack the posterior supraalar bristle (pSA), 60% the anterior dorsocentral bristles (aDC) and 1% the posterior supraalar bristle (pSA). In the wing disc of dally^ΔP-527/dally^gem animals, the number of sensory organ mother cells (SMCs) for chemosensory bristles at the anterior wing margin is severely reduced, as compared to wild-type discs. An absence of the SMCs for the aDC, pSA are also observed in these wing discs. The size of the TSM proneural cluster is severely decreased in these mutants, whilst the L3 proneural cluster remains unaffected.

(Fujise et al., 2001)

16% of dally^gem/dally^ΔP-188 mutant embryos have cuticle defects. About 90% of dally^ΔP-188/dally^gem flies show abnormalities in their genitalia. About 70% of dally⁰⁶⁴⁶⁴/dally^gem flies show abnormalities in their genitalia.

(Tsuda et al., 1999)

Homozygous males and females display a genitalia defective phenotype, all or parts of the genital disc derivatives are missing. In addition flies have abnormal wings, L5 is incomplete.

(Chorsky and Belote, 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

dally^gem has increased mortality during development phenotype, enhanceable by dlp^ΔGAG

(Nakato et al., 2024)

dally^gem has visible | adult stage phenotype, enhanceable by wdp^KO.ΔCDS/wdp^KO.ΔCDS

(Koh et al., 2023)

dally^gem has visible | adult stage phenotype, enhanceable by nord^Δ1728/nord^Δ1728

(Akiyama et al., 2022)

dally^gem has decreased size | adult stage phenotype, enhanceable by nord^Δ1728/nord^Δ1728

(Akiyama et al., 2022)

NOT Enhanced by

Statement

Reference

dally^gem has increased cell death | larval stage | dominant phenotype, non-enhanceable by Scer\GAL4^C-765/magu^UAS.Tag:V5

(Merino et al., 2022)

Suppressed by

Statement

Reference

dally^gem has increased cell death | dominant | larval stage phenotype, suppressible by Scer\GAL4^C-765/fwe^KK109356

(Merino et al., 2022)

NOT suppressed by

Statement

Reference

dally^gem has increased cell death | dominant | larval stage phenotype, non-suppressible by Scer\GAL4^C-765/magu^UAS.Tag:V5

(Merino et al., 2022)

NOT Enhancer of

Statement

Reference

dally^gem/dally[+] is a non-enhancer of visible | dominant phenotype of sog^EP7

(Araujo et al., 2003)

dally^gem/dally[+] is a non-enhancer of visible | dominant phenotype of sog^EP11

(Araujo et al., 2003)

Suppressor of

Statement

Reference

dally^gem is a suppressor of visible phenotype of Sulf1^ΔP1

(Kleinschmit et al., 2010)

NOT Suppressor of

Statement

Reference

dally^gem/dally[+] is a non-suppressor of visible | dominant phenotype of sog^EP11

(Araujo et al., 2003)

dally^gem/dally[+] is a non-suppressor of visible | dominant phenotype of sog^EP7

(Araujo et al., 2003)

Other

Statement

Reference

dally^gem, dlp^ΔGAG has lethal phenotype

(Nakato et al., 2024)

dally^gem, wdp^KO.ΔCDS has visible | adult stage phenotype

(Koh et al., 2023)

Hsepi^d12, dally^gem has lethal phenotype

(Dejima et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

dally^gem has male genitalia | absent phenotype, enhanceable by wdp^KO.ΔCDS/wdp^KO.ΔCDS

(Koh et al., 2023)

dally^gem has longitudinal vein phenotype, enhanceable by wdp^KO.ΔCDS/wdp^KO.ΔCDS

(Koh et al., 2023)

dally^gem has wing phenotype, enhanceable by nord^Δ1728/nord^Δ1728

(Akiyama et al., 2022)

dally^ΔP-527/dally^gem has posterior postalar bristle phenotype, enhanceable by sll^7E18

(Luders et al., 2003)

dally^gem/dally⁰⁶⁴⁶⁴ has anterior dorsocentral bristle phenotype, enhanceable by put¹⁰⁴⁶⁰

(Fujise et al., 2001)

dally^gem/dally⁰⁶⁴⁶⁴ has posterior supraalar bristle phenotype, enhanceable by put¹⁰⁴⁶⁰

(Fujise et al., 2001)

dally^gem/dally⁰⁶⁴⁶⁴ has posterior supraalar bristle phenotype, enhanceable by wg^l-17

(Fujise et al., 2001)

dally^ΔP-188/dally^gem has embryonic epidermis phenotype, enhanceable by wg^l-12/wg[+]

(Tsuda et al., 1999)

NOT Enhanced by

Statement

Reference

dally^gem has wing margin phenotype, non-enhanceable by wdp^KO.ΔCDS/wdp^KO.ΔCDS

(Koh et al., 2023)

dally^gem has wing disc | larval stage phenotype, non-enhanceable by Scer\GAL4^C-765/magu^UAS.Tag:V5

(Merino et al., 2022)

Suppressed by

Statement

Reference

dally^gem has wing vein L5 phenotype, suppressible | partially by nord[+]/nord^Δ1728

(Akiyama et al., 2022)

dally^gem has wing vein L5 phenotype, suppressible by nord^Δ1728/nord^Δ1728

(Akiyama et al., 2022)

dally^gem has wing disc | larval stage phenotype, suppressible by Scer\GAL4^C-765/fwe^KK109356

(Merino et al., 2022)

dally^gem has female germline stem cell phenotype, suppressible by Dad^P1883

(Guo and Wang, 2009)

dally^gem has female germline stem cell phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/tkv^ca.UASp

(Guo and Wang, 2009)

dally^ΔP-188/dally^gem has genitalia phenotype, suppressible by wg^l-12/wg[+]

(Tsuda et al., 1999)

dally^gem/dally⁰⁶⁴⁶⁴ has genitalia phenotype, suppressible by wg[+]/wg^l-17

(Tsuda et al., 1999)

NOT suppressed by

Statement

Reference

dally^gem has wing margin phenotype, non-suppressible by wdp^KO.ΔCDS/wdp^KO.ΔCDS

(Koh et al., 2023)

dally^gem has wing disc | larval stage phenotype, non-suppressible by Scer\GAL4^C-765/magu^UAS.Tag:V5

(Merino et al., 2022)

NOT Enhancer of

Statement

Reference

dally^gem/dally[+] is a non-enhancer of wing vein phenotype of sog^EP7

(Araujo et al., 2003)

dally^gem/dally[+] is a non-enhancer of wing vein phenotype of sog^EP11

(Araujo et al., 2003)

Suppressor of

Statement

Reference

dally^gem is a suppressor of wing margin bristle | increased number phenotype of Sulf1^ΔP1

(Kleinschmit et al., 2010)

dally^gem is a suppressor of anterior wing margin phenotype of Sulf1^ΔP1

(Kleinschmit et al., 2010)

NOT Suppressor of

Statement

Reference

dally^gem/dally[+] is a non-suppressor of wing vein phenotype of sog^EP11

(Araujo et al., 2003)

dally^gem/dally[+] is a non-suppressor of wing vein phenotype of sog^EP7

(Araujo et al., 2003)

Other

Statement

Reference

dally^gem, wdp^KO.ΔCDS has wing phenotype

(Koh et al., 2023)

dally^gem, nord^Δ1728 has gonad | male | larval stage phenotype

(Akiyama et al., 2022)

dally^gem, sgl^N71/sgl[+] has embryonic epidermis phenotype

(Tsuda et al., 1999)

dally^gem, sgl^N71 has embryonic epidermis phenotype

(Tsuda et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

A heterozygous dally^gem mutant in a Hsepi^d12 homozygous background results in a decreased number of chemosensory bristles.

Hsepi^d12; dally^gem double mutants are lethal.

(Dejima et al., 2013)

dally^gem, Sulf1^ΔP1 mutant animals display the same reduced number of chemosensory and mechanosensory bristles at the anterior dorsal wing margin as dally^gem single mutants.

(Kleinschmit et al., 2010)

Dad^P1883 partially suppresses the loss of germline stem cells which is seen in the ovarioles of dally^gem females.

Expression of tkv^{ca.Scer\UAS.P\T} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} suppresses the loss of germline stem cells which is seen in the ovarioles of dally^gem females.

(Guo and Wang, 2009)

The addition of sll^7E18 to dally^gem/dally^ΔP-527 animals enhances the posterior postalar bristle phenotype - 30% of individuals show this phenotype.

(Luders et al., 2003)

The addition of wg^l-17 to dally^gem/dally⁰⁶⁴⁶⁴ flies, leads to enhancement of the posterior supraalar bristle (pSA) phenotype seen in dally^gem/dally⁰⁶⁴⁶⁴ flies alone: 30% of flies have the phenotype, rather than 1%. The addition of put¹⁰⁴⁶⁰ to dally^gem/dally⁰⁶⁴⁶⁴ flies, leads to enhancement of the anterior dorsocentral bristle (aDC) phenotype seen in dally^gem/dally⁰⁶⁴⁶⁴ flies alone: 90% of flies have the phenotype, rather than 24%. The pSA bristle phenotype is also enhanced by the addition of put¹⁰⁴⁶⁰. All flies have the phenotype, rather than 86%. The addition of dally^gem/+ enhances the pSA phenotype of Tp(3;3)iro-1 or Df(3L)iro-DFM3/+ flies.

(Fujise et al., 2001)

Heterozygosity for wg^l-12 increases the penetrance of cuticle defects in dally^ΔP-188/dally^gem embryos from 16% to 41%. Embryos heterozygous for dally^gem derived from mothers heterozygous for sgl^N71 show cuticle defects characteristic of a reduction in wg signalling. The abnormal genitalia phenotype of dally^ΔP-188/dally^gem flies is dominantly suppressed (down to less than 5% penetrance) by wg^l-12. The abnormal genitalia phenotype of dally⁰⁶⁴⁶⁴/dally^gem flies is dominantly suppressed (down to less than 10% penetrance) by wg^l-17.

(Tsuda et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

dally^unspecified

(Selleck, 1999.11.2)

Rescued by

Df(3L)Scf-R6/dally^gem is rescued by dally^UAS.cJa/Scer\GAL4^A9

(Kirkpatrick et al., 2006)

dally^gem is rescued by dally^UAS.cJa/Scer\GAL4^A9

(Kirkpatrick et al., 2006)

dally^gem is rescued by Scer\GAL4^A9/dally^{tm.UAS.Tag:MYC}

(Takeo et al., 2005)

dally^gem is rescued by Scer\GAL4^A9/dally^UAS.Tag:MYC

(Takeo et al., 2005)

Partially rescued by

dally^gem is partially rescued by dally^UAS.cJa/Scer\GAL4^bab1-Pgal4-2

(Guo and Wang, 2009)

dally^gem is partially rescued by dally^UAS.cJa/Scer\GAL4^en-e16E

(Guo and Wang, 2009)

dally^gem is partially rescued by dally^UAS.cJa/Scer\GAL4^C587

(Guo and Wang, 2009)

dally^ΔP-527/dally^gem is partially rescued by Scer\GAL4^A9/dally^{ΔGAG.UAS.Tag:MYC}

(Kirkpatrick et al., 2006)

dally^ΔP-527/dally^gem is partially rescued by Scer\GAL4^A9/dally^ΔGAG.UAS

(Kirkpatrick et al., 2006)

Df(3L)Scf-R6/dally^gem is partially rescued by Scer\GAL4^A9/dally^ΔGAG.UAS

(Kirkpatrick et al., 2006)

dally^gem is partially rescued by Scer\GAL4^A9/dally^{ΔGAG.UAS.Tag:MYC}

(Kirkpatrick et al., 2006)

dally^gem is partially rescued by Scer\GAL4^A9/dally^ΔGAG.UAS

(Kirkpatrick et al., 2006)

dally^gem is partially rescued by dally^{sec.UAS.Tag:MYC}/Scer\GAL4^A9

(Takeo et al., 2005)

Comments

Expression of dally^{ΔGAG.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^A9 restores the L5 wing vein in 35% of dally^gem homozygotes and 76% of dally^gem/dally^ΔP-527 transheterozygotes. It performs a slight rescue of the number of chemosensory bristles in dally^gem/dally^ΔP-527 flies but does not restore posterior supraalar bristles.

Expression of dally^{ΔGAG.Scer\UAS} under the control of Scer\GAL4^A9 partially rescues the phenotypes of dally^gem, dally^gem/Df(3L)Scf-R6, and dally^gem/dally^ΔP-527 mutants. The transgene substantially restores chemosensory bristle numbers in the wing margin, almost fully rescues the formation of wing vein L5, almost fully restores the formation of the anterior dorsocentral bristles on the notum without inducing the formation of extra ones for all mutants. However, it fails to restore formation of the posterior supraalar bristles.

(Kirkpatrick et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

dally^gem

(Liu et al., 2024, Nakato et al., 2024, Koh et al., 2023, Akiyama et al., 2022, Merino et al., 2022, Romanova-Michaelides et al., 2022, Zhao et al., 2020, Kanai et al., 2018, Dejima et al., 2013, Xia et al., 2012, Dejima et al., 2011, Wojcinski et al., 2011, Kleinschmit et al., 2010, Guo and Wang, 2009, Akiyama et al., 2008, Kirkpatrick et al., 2006)

gem

(Selleck, 1999.11.2)

Name Synonyms

Secondary FlyBase IDs

FBal0039364

References (24)

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