FlyBase Allele Report: Dmel\Rbf[HMS03004]

General Information

Symbol

Dmel\Rbf^HMS03004

Species

D. melanogaster

Name

FlyBase ID

FBal0266576

Feature type

allele

Associated gene

Dmel\Rbf

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS03004}

Also Known As

UAS-Rbf RNAi, RBF1 RNAi, UAS-Rbf1^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

(Ni et al., 2011.7.19)

Progenitor genotype

Carried in construct

P{TRiP.HMS03004}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS03004

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA

(Parkhitko et al., 2021)

CEA with Pten^HMS00044, Ras85D^G13Q.UAS

(Parkhitko et al., 2021)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of cancer

is exacerbated by Nkap^HMS00995

(Parkhitko et al., 2021)

is exacerbated by qkr58E-2^HMC03966

(Parkhitko et al., 2021)

is exacerbated by α-Spec^HMC04371

(Parkhitko et al., 2021)

is exacerbated by CG7483^HMS00696

(Parkhitko et al., 2021)

is exacerbated by Cdc27^HM04024

(Parkhitko et al., 2021)

is exacerbated by Ada2a^HMJ03126

(Parkhitko et al., 2021)

is exacerbated by Blos2^HMJ21554

(Parkhitko et al., 2021)

is exacerbated by CG10600^HMS05767

(Parkhitko et al., 2021)

is exacerbated by CG16903^HMC06024

(Parkhitko et al., 2021)

is exacerbated by CG31871^HMS05379

(Parkhitko et al., 2021)

is exacerbated by CG6015^HMS01037

(Parkhitko et al., 2021)

is exacerbated by Mfap1^HM05216

(Parkhitko et al., 2021)

is exacerbated by Cdc5^HMC04732

(Parkhitko et al., 2021)

is exacerbated by Mi-2^HMS00301

(Parkhitko et al., 2021)

is exacerbated by Cse1^JF02972

(Parkhitko et al., 2021)

is exacerbated by Nipped-B^HM05172

(Parkhitko et al., 2021)

is exacerbated by Cul3^HMS01572

(Parkhitko et al., 2021)

is exacerbated by Non1^JF01590

(Parkhitko et al., 2021)

is exacerbated by Not1^HMS00526

(Parkhitko et al., 2021)

is exacerbated by Pgam1^HMC04818

(Parkhitko et al., 2021)

is exacerbated by Su(var)2-10^HMS00750

(Parkhitko et al., 2021)

is exacerbated by Sumo^JF02869

(Parkhitko et al., 2021)

is exacerbated by Taf1^HMS00416

(Parkhitko et al., 2021)

is exacerbated by Cul4^HMC02981

(Parkhitko et al., 2021)

is exacerbated by Tango1^HMS05801

(Parkhitko et al., 2021)

is exacerbated by Pp1-87B^HMS00409

(Parkhitko et al., 2021)

is exacerbated by Pp2A-29B^JF03316

(Parkhitko et al., 2021)

is exacerbated by CycA^JF02472

(Parkhitko et al., 2021)

is exacerbated by l(1)10Bb^HMS00255

(Parkhitko et al., 2021)

is exacerbated by Pp4-19C^HMJ21831

(Parkhitko et al., 2021)

is exacerbated by Tlk^HMS00943

(Parkhitko et al., 2021)

is exacerbated by Dim1^HMC03851

(Parkhitko et al., 2021)

is exacerbated by U2af38^HMS04505

(Parkhitko et al., 2021)

is exacerbated by Prosα7^HMS00068

(Parkhitko et al., 2021)

is exacerbated by U2af50^HMC03810

(Parkhitko et al., 2021)

is exacerbated by Dis3^HMJ24000

(Parkhitko et al., 2021)

is exacerbated by U4-U6-60K^HMC03931

(Parkhitko et al., 2021)

is exacerbated by Ran^HMS02885

(Parkhitko et al., 2021)

is exacerbated by Dlg5^HMJ23170

(Parkhitko et al., 2021)

is exacerbated by Rm62^JF01385

(Parkhitko et al., 2021)

is exacerbated by E(Pc)^JF03101

(Parkhitko et al., 2021)

is exacerbated by Uba1^JF01977

(Parkhitko et al., 2021)

is exacerbated by RpS29^HMJ21541

(Parkhitko et al., 2021)

is exacerbated by RpS4^HMS05869

(Parkhitko et al., 2021)

is exacerbated by HDAC1^JF01401

(Parkhitko et al., 2021)

is exacerbated by Rpn8^HMS05743

(Parkhitko et al., 2021)

is exacerbated by Hsc70-1^HMS00888

(Parkhitko et al., 2021)

is exacerbated by Ubc6^HMS02466

(Parkhitko et al., 2021)

is exacerbated by VhaAC39-1^HMS01442

(Parkhitko et al., 2021)

is exacerbated by Rpn9^HMS01007

(Parkhitko et al., 2021)

is exacerbated by Hsc70-4^HMS00152

(Parkhitko et al., 2021)

is exacerbated by Sce^HMS05745

(Parkhitko et al., 2021)

is exacerbated by dom^HMS02208

(Parkhitko et al., 2021)

is exacerbated by eIF3m^HMS00667

(Parkhitko et al., 2021)

is exacerbated by eIF4G1^HMS00762

(Parkhitko et al., 2021)

is exacerbated by Sec31^HMS00666

(Parkhitko et al., 2021)

is exacerbated by gpp^JF01283

(Parkhitko et al., 2021)

is exacerbated by KCNQ^JF02562

(Parkhitko et al., 2021)

is exacerbated by jub^HMS02335

(Parkhitko et al., 2021)

is exacerbated by SkpA^HMS00791

(Parkhitko et al., 2021)

is exacerbated by SmF^JF02276

(Parkhitko et al., 2021)

is exacerbated by Kdm5^HM05155

(Parkhitko et al., 2021)

is exacerbated by kin17^HMC03906

(Parkhitko et al., 2021)

is exacerbated by pyd^HMS00263

(Parkhitko et al., 2021)

is exacerbated by kto^HMS01062

(Parkhitko et al., 2021)

is exacerbated by lqf^HMJ22079

(Parkhitko et al., 2021)

is exacerbated by mRNA-cap^HMS04494

(Parkhitko et al., 2021)

is exacerbated by Snap29^JF01883

(Parkhitko et al., 2021)

is exacerbated by Spt6^HMS00364

(Parkhitko et al., 2021)

is exacerbated by Su(H)^HMS05748

(Parkhitko et al., 2021)

is exacerbated by pea^HMS00528

(Parkhitko et al., 2021)

is exacerbated by sktl^JF02796

(Parkhitko et al., 2021)

is exacerbated by snRNP-U1-70K^HMS00274

(Parkhitko et al., 2021)

is exacerbated by snRNP-U1-C^HMC04045

(Parkhitko et al., 2021)

is exacerbated by sqh^HMS00437

(Parkhitko et al., 2021)

is exacerbated by sv^JF02582

(Parkhitko et al., 2021)

is exacerbated by trr^HMS01019

(Parkhitko et al., 2021)

is exacerbated by trx^HMS00580

(Parkhitko et al., 2021)

is exacerbated by tum^HMS01417

(Parkhitko et al., 2021)

is exacerbated by wapl^HMJ22638

(Parkhitko et al., 2021)

is exacerbated by wupA^JF02172

(Parkhitko et al., 2021)

is ameliorated by Naa20A^GL01093

(Sheng and Du, 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell cycle | adult stage | male, with Scer\GAL4^esg.PU

(Sekar et al., 2023)

abnormal cell number | somatic clone | spermatogenesis, with Scer\GAL4^Tub.PU

(Sainz de la Maza et al., 2022)

abnormal cell number | spermatogenesis, with Scer\GAL4^C587

(Sainz de la Maza et al., 2022)

abnormal cell number | spermatogenesis, with Scer\GAL4^NP1624

(Sainz de la Maza et al., 2022)

increased cell death | larval stage | somatic clone, with Scer\GAL4^Act.PU

(Sheng and Du, 2020)

increased cell death | somatic clone, with Scer\GAL4^unspecified

(Zhang et al., 2014)

increased cell growth rate | adult stage | male, with Scer\GAL4^esg.PU

(Sekar et al., 2023)

increased occurrence of cell division | adult stage, with Scer\GAL4^esg.PU

(Zhao et al., 2022)

increased occurrence of cell division | spermatogenesis, with Scer\GAL4^NP1624

(Sainz de la Maza et al., 2022)

visible | adult stage | somatic clone, with Scer\GAL4^Act.PU

(Sheng et al., 2016)

Phenotype Manifest In

Detailed Description

Statement

Reference

Adulthood-only expression of Rbf^HMS03004 under the control of Scer\GAL4^esg.PU leads to an increase in the number of intestinal progenitors (esg-positive ISCs and EBs) in the adult posterior midgut epithelium.

(Zhao et al., 2022)

Full eye disc clones expressing Rbf^HMS03004 under the control of Scer\GAL4^Act.PU show apoptosis .

(Sheng and Du, 2020)

Expression of Rbf^HMS03004 RNAi under the control of Scer\GAL4^Act.PU in somatic clones induced specifically in the eye results in moderately reduced adult eye size compared to controls.

(Sheng et al., 2016)

Expression of Rbf^HMS03004 RNAi under the control of Scer\GAL4^nub.PU (using UAS-Dicer2 to enhance the RNAi efficiency) does not cause any apparent adult wing defects.

(Miles et al., 2014)

Expression of Rbf^HMS03004 in eye disc clones under the control of Scer\GAL4^unspecified induces apoptosis anterior to the morphogenetic furrow.

(Zhang et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Rbf^HMS03004, Scer\GAL4^Act.PU has increased cell death | larval stage | somatic clone phenotype, enhanceable by Naa20A^GL01093, Scer\GAL4^Act.PU

(Sheng and Du, 2020)

Rbf^HMS03004, Scer\GAL4^Act.PU has visible | adult stage | somatic clone phenotype, enhanceable by Stam^HMS01429, Scer\GAL4^Act.PU

(Sheng et al., 2016)

Rbf^HMS03004, Scer\GAL4^Act.PU has visible | adult stage | somatic clone phenotype, enhanceable by Hrs^JF02860, Scer\GAL4^Act.PU

(Sheng et al., 2016)

Rbf^HMS03004, Scer\GAL4^unspecified has increased cell death | somatic clone phenotype, enhanceable by sgg^DN.UAS.cUa, Scer\GAL4^unspecified

(Zhang et al., 2014)

Rbf^HMS03004, Scer\GAL4^unspecified has increased cell death | somatic clone phenotype, enhanceable by arm^{S10.UAS.Tag:MYC}, Scer\GAL4^unspecified

(Zhang et al., 2014)

Suppressed by

Statement

Reference

Rbf^HMS03004, Scer\GAL4^Tub.PU has abnormal cell number | somatic clone | spermatogenesis phenotype, suppressible by Dp^a3/Dp^a3

(Sainz de la Maza et al., 2022)

Rbf^HMS03004, Scer\GAL4^NP1624 has abnormal cell number | somatic clone | spermatogenesis phenotype, suppressible by Dp^GD4444, Scer\GAL4^NP1624

(Sainz de la Maza et al., 2022)

Rbf^HMS03004, Scer\GAL4^NP1624 has abnormal cell number | spermatogenesis phenotype, suppressible by srl^UAS.cUa/Ets97D^UAS.Tag:HA, Scer\GAL4^NP1624

(Sainz de la Maza et al., 2022)

Rbf^HMS03004, Scer\GAL4^Tub.PU has abnormal cell number | somatic clone | spermatogenesis phenotype, suppressible | partially by Ets97D^UAS.Tag:HA, Scer\GAL4^Tub.PU

(Sainz de la Maza et al., 2022)

Rbf^HMS03004, Scer\GAL4^Tub.PU has abnormal cell number | somatic clone | spermatogenesis phenotype, suppressible | partially by srl^UAS.cUa, Scer\GAL4^Tub.PU

(Sainz de la Maza et al., 2022)

Suppressor of

Statement

Reference

Rbf^HMS03004, Scer\GAL4^esg.PU is a suppressor of decreased cell number | adult stage phenotype of Phb1^HMS00702, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Rbf^HMS03004, Scer\GAL4^esg.PU is a suppressor of decreased cell number | adult stage phenotype of Phb2^GD8538, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Other

Statement

Reference

Ets97D^UAS.Tag:HA, Rbf^HMS03004, Scer\GAL4^NP1624, srl^UAS.cUa has increased occurrence of cell division | spermatogenesis phenotype

(Sainz de la Maza et al., 2022)

Naa20A^GL01093, Rbf^HMS03004, Scer\GAL4^Act.PU has decreased size | somatic clone | adult stage phenotype

(Sheng and Du, 2020)

Rbf^HMS03004, Scer\GAL4^nub.PU, pum^GD14303 has visible | adult stage phenotype

(Miles et al., 2014)

Rbf^HMS03004, Scer\GAL4^nub.PU, nanos^j3B6 has visible | adult stage phenotype

(Miles et al., 2014)

Rbf^HMS03004, Scer\GAL4^nub.PU, brat^GD7073 has visible | adult stage phenotype

(Miles et al., 2014)

Rbf^HMS03004, Scer\GAL4^nub.PU, brat¹ has visible | adult stage phenotype

(Miles et al., 2014)

Rbf^HMS03004, Scer\GAL4^nub.PU, pum^HMS01564 has visible | adult stage phenotype

(Miles et al., 2014)

Rbf^HMS03004, Scer\GAL4^nub.PU, brat^ts1 has visible | adult stage phenotype

(Miles et al., 2014)

Rbf^HMS03004, Scer\GAL4^nub.PU, pum³ has visible | adult stage phenotype

(Miles et al., 2014)

Rbf^HMS03004, Scer\GAL4^nub.PU, nanos^JF02931 has visible | adult stage phenotype

(Miles et al., 2014)

Rbf^HMS03004, Scer\GAL4^nub.PU, nanos^HMS00785 has visible | adult stage phenotype

(Miles et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

Rbf^HMS03004, Scer\GAL4^Act.PU has eye disc | somatic clone phenotype, enhanceable by Naa20A^GL01093, Scer\GAL4^Act.PU

(Sheng and Du, 2020)

Rbf^HMS03004, Scer\GAL4^Act.PU has eye | somatic clone phenotype, enhanceable by Stam^HMS01429, Scer\GAL4^Act.PU

(Sheng et al., 2016)

Rbf^HMS03004, Scer\GAL4^Act.PU has eye | somatic clone phenotype, enhanceable by Hrs^JF02860, Scer\GAL4^Act.PU

(Sheng et al., 2016)

Rbf^HMS03004, Scer\GAL4^unspecified has eye disc | somatic clone phenotype, enhanceable by sgg^DN.UAS.cUa, Scer\GAL4^unspecified

(Zhang et al., 2014)

Rbf^HMS03004, Scer\GAL4^unspecified has eye disc | somatic clone phenotype, enhanceable by arm^{S10.UAS.Tag:MYC}, Scer\GAL4^unspecified

(Zhang et al., 2014)

Suppressed by

Statement

Reference

Rbf^HMS03004, Scer\GAL4^Tub.PU has somatic cell of testis | somatic clone phenotype, suppressible by Dp^a3/Dp^a3

(Sainz de la Maza et al., 2022)

Rbf^HMS03004, Scer\GAL4^NP1624 has somatic cell of testis | somatic clone phenotype, suppressible by Dp^GD4444, Scer\GAL4^NP1624

(Sainz de la Maza et al., 2022)

Rbf^HMS03004, Scer\GAL4^NP1624 has somatic cell of testis phenotype, suppressible | partially by srl^UAS.cUa/Ets97D^UAS.Tag:HA, Scer\GAL4^NP1624

(Sainz de la Maza et al., 2022)

Rbf^HMS03004, Scer\GAL4^NP1624 has mitochondrion | spermatogenesis phenotype, suppressible | partially by srl^UAS.cUa/Ets97D^UAS.Tag:HA, Scer\GAL4^NP1624

(Sainz de la Maza et al., 2022)

Rbf^HMS03004, Scer\GAL4^Tub.PU has somatic cell of testis | somatic clone phenotype, suppressible | partially by Ets97D^UAS.Tag:HA, Scer\GAL4^Tub.PU

(Sainz de la Maza et al., 2022)

Rbf^HMS03004, Scer\GAL4^Tub.PU has somatic cell of testis | somatic clone phenotype, suppressible | partially by srl^UAS.cUa, Scer\GAL4^Tub.PU

(Sainz de la Maza et al., 2022)

Suppressor of

Statement

Reference

Rbf^HMS03004, Scer\GAL4^esg.PU is a suppressor of adult posterior midgut epithelium phenotype of Phb1^HMS00702, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Rbf^HMS03004, Scer\GAL4^esg.PU is a suppressor of adult posterior midgut epithelium phenotype of Phb2^GD8538, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Other

Statement

Reference

Ets97D^UAS.Tag:HA, Rbf^HMS03004, Scer\GAL4^NP1624, srl^UAS.cUa has somatic cell of testis phenotype

(Sainz de la Maza et al., 2022)

Ets97D^UAS.Tag:HA, Rbf^HMS03004, Scer\GAL4^NP1624, srl^UAS.cUa has nucleus | spermatogenesis phenotype

(Sainz de la Maza et al., 2022)

Naa20A^GL01093, Rbf^HMS03004, Scer\GAL4^Act.PU has eye | somatic clone phenotype

(Sheng and Du, 2020)

Rbf^HMS03004, Scer\GAL4^nub.PU, pum^GD14303 has wing blade phenotype

(Miles et al., 2014)

Rbf^HMS03004, Scer\GAL4^nub.PU, nanos^HMS00785 has wing blade phenotype

(Miles et al., 2014)

Rbf^HMS03004, Scer\GAL4^nub.PU, nanos^j3B6 has wing vein | ectopic phenotype

(Miles et al., 2014)

Rbf^HMS03004, Scer\GAL4^nub.PU, nanos^j3B6 has wing blade phenotype

(Miles et al., 2014)

Rbf^HMS03004, Scer\GAL4^nub.PU, brat^GD7073 has wing blade phenotype

(Miles et al., 2014)

Rbf^HMS03004, Scer\GAL4^nub.PU, brat¹ has wing vein | ectopic phenotype

(Miles et al., 2014)

Rbf^HMS03004, Scer\GAL4^nub.PU, brat¹ has wing blade phenotype

(Miles et al., 2014)

Rbf^HMS03004, Scer\GAL4^nub.PU, brat^ts1 has wing vein | ectopic phenotype

(Miles et al., 2014)

Rbf^HMS03004, Scer\GAL4^nub.PU, brat^ts1 has wing blade phenotype

(Miles et al., 2014)

Rbf^HMS03004, Scer\GAL4^nub.PU, pum^HMS01564 has wing blade phenotype

(Miles et al., 2014)

Rbf^HMS03004, Scer\GAL4^nub.PU, pum³ has wing blade phenotype

(Miles et al., 2014)

Rbf^HMS03004, Scer\GAL4^nub.PU, nanos^JF02931 has wing blade phenotype

(Miles et al., 2014)

Rbf^HMS03004, Scer\GAL4^nub.PU, nanos^HMS00785 has wing vein | ectopic phenotype

(Miles et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The induction of full eye clones co-expressing Rbf^HMS03004 and Naa20A^GL01093 under the control of Scer\GAL4^Act.PU results in a small adult eye.

(Sheng and Du, 2020)

The smaller adult eye size characteristic for animals expressing Rbf^HMS03004 RNAi under the control of the Scer\GAL4^Act.PU driver in somatic clones induced specifically in the eye can be reduced further by co-expression of either Stam^HMS01429 or Hrs^JF02860 RNAi.

(Sheng et al., 2016)

Expression of Rbf^HMS03004 RNAi under the control of Scer\GAL4^nub.PU (using UAS-Dicer to enhance RNAi efficiency) in combination with any of the following: pum^GD14303, pum^HMS01564, pum³, nos^JF02931, nos^HMS00785, nos^j3B6, brat^GD7073, brat¹ or brat^ts1 results in adult wing defects of varying severity (ectopic venation, blistering and/or wing blade deformation). Combination with nos^L7 has no apparent wing phenotype.

(Miles et al., 2014)

Co-expression of sgg^{DN.Scer\UAS.cUa} enhances the apoptosis seen in eye disc clones expressing Rbf^HMS03004 under the control of a GAL4 driver.

Co-expression of arm^{S10.Scer\UAS.T:Hsap\MYC} enhances the apoptosis seen in eye disc clones expressing Rbf^HMS03004 under the control of a GAL4 driver.

(Zhang et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

36744