FlyBase Gene Report: Dmel\Ptp99A

General Information

Symbol

Dmel\Ptp99A

Species

D. melanogaster

Name

Protein tyrosine phosphatase 99A

Annotation Symbol

CG11516

Feature Type

protein_coding_gene

FlyBase ID

FBgn0004369

Gene Model Status

Current

Stock Availability

123 publicly available

Enzyme Name (EC)

protein-tyrosine-phosphatase (3.1.3.48)

Gene Summary

Protein tyrosine phosphatase 99A (Ptp99A) encodes a transmembrane receptor tyrosine phosphatase. Its single mutants have no known phenotypes, but Ptp99A synergizes with Ptp69D to increase the penetrance of the Ptp69D motor axon defects. Ptp99A mutations also suppress the 'bypass' motor axon phenotype produced by Lar mutations. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

DPTP99A

Key Links

Genomic Location

Cytogenetic map

99A7-99B1

Sequence location

3R:29,377,646..29,487,131 [+]

Recombination map

3-98

RefSeq locus

NT_033777 REGION:29377646..29487131

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (9 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein tyrosine phosphatase activity

inferred from direct assay

(Madan et al., 2011)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables protein tyrosine phosphatase activity

inferred from biological aspect of ancestor with PANTHER:PTN008567661

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity with UniProtKB:P23470

(Hariharan et al., 1991)

inferred from electronic annotation with InterPro:IPR000242

(InterPro Project Members, 2004-)

enables transmembrane receptor protein tyrosine phosphatase activity

inferred from sequence or structural similarity

(Yang et al., 1991)

Biological Process (5 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in defasciculation of motor neuron axon

inferred from genetic interaction with FLYBASE:Ptp69D; FB:FBgn0014007

(Desai et al., 1996)

involved_in motor neuron axon guidance

inferred from genetic interaction with FLYBASE:Lar; FB:FBgn0000464,FLYBASE:Ptp10D; FB:FBgn0004370

(Sun et al., 2001)

inferred from genetic interaction with FLYBASE:Ptp69D; FB:FBgn0014007

(Desai et al., 1996)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in axonal defasciculation

traceable author statement

(Van Vactor et al., 1998)

involved_in neuron projection development

inferred from biological aspect of ancestor with PANTHER:PTN000439254

(GO Reference Genome Project, 2011-)

involved_in signal transduction

inferred from biological aspect of ancestor with PANTHER:PTN008567661

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in axon

inferred from direct assay

(Jeon et al., 2008)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in plasma membrane

inferred from sequence or structural similarity

(Hariharan et al., 1991, Tian et al., 1991, Yang et al., 1991)

Gene Group (FlyBase)

RECEPTOR PROTEIN TYROSINE PHOSPHATASES

Protein Family (UniProt)

Belongs to the protein-tyrosine phosphatase family. Receptor class subfamily. (P35832)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

protein tyrosine phosphatase activity

O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate (3.1.3.48)

RHEA 10684:

Summaries

Gene Snapshot

Gene Group (FlyBase)

RECEPTOR PROTEIN TYROSINE PHOSPHATASES -: Receptor Protein Tyrosine Phosphatases possess a single transmembrane domain and are localized to the plasma membrane. The Protein Tyrosine Phosphatases are defined by the active-site signature motif Cys-X5-Arg. (Adapted from FBrf0129980 and PMID:17057753).

Protein Function (UniProtKB)

May play a key role in signal transduction and growth control. May have a role in the establishment of the intersegmental and segmental nerves.

(UniProt, P35832)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Ptp99A for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P35832)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Ptp99A-RA

FBtr0085422

NM_170409

6223

1226

Ptp99A-RB

FBtr0085423

NM_057468

4340

1297

Ptp99A-RC

FBtr0085424

NM_206579

6268

1241

Ptp99A-RF

FBtr0303148

NM_143434

7109

1397

Ptp99A-RG

FBtr0303454

NM_001202380

5793

922

Additional Transcript Data and Comments

Reported size (kB)

7.5, 6.0, 5.0, 3.7 (northern blot)

(Hariharan et al., 1991)

7.3, 6.2, 4.8 (northern blot)

(Tian et al., 1991)

7.9, 6.9, 6.4, 6, 5.4, 4.4, 3.9 (northern blot)

(Yang et al., 1991)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Ptp99A-PA

FBpp0084791

136.9

1226

6.45

NP_733288

AAF56891

Ptp99A-PB

FBpp0084792

145.1

1297

6.41

NP_476816

AAN14172

Ptp99A-PC

FBpp0089274

138.7

1241

6.45

NP_996302

AAS65222

Ptp99A-PF

FBpp0292247

157.3

1397

6.75

FBpp0292507

103.9

922

6.72

A0A0B4JDF4

NP_001189309

ADV37399

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

13101 (aa); 145 (kD predicted)

(Hariharan et al., 1991)

1231 (aa); 137 (kD predicted)

(Tian et al., 1991)

1231 (aa); 137, 120 (kD)

(Yang et al., 1991)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ptp99A using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.20

Transcript Expression

expression microarray

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult fat body

Comment: cyclical, peak ZT21-0

(Xu et al., 2011)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

presumptive embryonic/larval central nervous system | segmentally repeated

(Tian et al., 1991)

embryonic stage 12

central nervous system | restricted

(Yang et al., 1991)

pCC neuron

(Yang et al., 1991)

embryonic stage 12 -- 14

larval RP2 motor neuron

(Yang et al., 1991)

larval DA1 motor neuron

(Yang et al., 1991)

larval VL3/4 motor neuron

(Yang et al., 1991)

larval VO2 motor neuron

(Yang et al., 1991)

embryonic stage 13 -- 16

larval midline neuron

(Kearney et al., 2004)

embryonic stage 13 -- 17

larval VUM neuron

(Yang et al., 1991)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(Hariharan et al., 1991, Tian et al., 1991, Yang et al., 1991)

embryonic stage & adult stage

(Yang et al., 1991)

embryonic stage 1 -- embryonic stage 17(i)

organism

(Tian et al., 1991)

larval stage

imaginal disc

(Hariharan et al., 1991)

first instar larval stage

(Yang et al., 1991)

adult stage

adult head

(Yang et al., 1991)

Additional Descriptive Data

Expressed cyclically in the adult fat body.

(Xu et al., 2011)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunohistochemistry

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic/larval tracheal section

(Tian et al., 1991)

embryonic stage 12 -- 17

symmetrical commissure

(Tian et al., 1991)

larval ventral nerve cord

(Tian et al., 1991)

larval longitudinal connective

(Tian et al., 1991)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 12

larval VO2 motor neuron

(Yang et al., 1991)

larval VL3/4 motor neuron

(Yang et al., 1991)

pCC neuron

(Yang et al., 1991)

central nervous system | restricted

(Yang et al., 1991)

embryonic stage 12 -- 14

larval DA1 motor neuron

(Yang et al., 1991)

larval RP2 motor neuron

(Yang et al., 1991)

embryonic stage 12 -- 16

larval intersegmental nerve

(Yang et al., 1991)

larval segmental nerve

(Yang et al., 1991)

embryonic stage 12 -- 17

symmetrical commissure

(Yang et al., 1991)

larval longitudinal connective

(Yang et al., 1991)

embryonic stage 13 -- 17

supraesophageal ganglion

(Hariharan et al., 1991)

larval longitudinal connective

(Hariharan et al., 1991)

presumptive embryonic/larval central nervous system

(Hariharan et al., 1991)

larval posterior commissure

(Hariharan et al., 1991)

larval anterior commissure

(Hariharan et al., 1991)

neuron

• axon

(Hariharan et al., 1991)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Localized Ptp99A protein is first observed in the axonal layer in stage 12 embryos. It is also expressed transiently in some neuronal cell bodies. By stage 14 uniform expression is seen along the commissures and longitudinal connectives. Weaker staining is seen on peripheral axon tracts near the edges of the CNS. Weak tracheal staining is sometimes observed.

(Tian et al., 1991)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in axon

inferred from direct assay

(Jeon et al., 2008)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Ptp99A in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Ptp99A

View exonic expression by tissue for Dmel\Ptp99A

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

43469/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004369

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004369

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004369

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

Flygut - An atlas of the Drosophila adult midgut

FBgn0004369

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 20 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 16 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Ptp99A

Transgenic constructs containing regulatory region of Ptp99A

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(3R)KE

(Desai et al., 1997)

Gene not disrupted in

Df(3R)Spase12^d4

(Haase Gilbert et al., 2013)

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

viable, with Scer\GAL4^Mef2.PR

viable, with Scer\GAL4^pnr-MD237

viable, with Scer\GAL4^tin.CΔ4

viable (with Ptp99A^HA64)

Ptp99A^R3

viable (with Ptp99A^R3)

Ptp99A^HA64

Sterility

Allele

female semi-sterile, with Scer\GAL4^{VP16.mat.αTub67C}

fertile

Ptp99A^EY08245

fertile (with Ptp99A^HA64)

Ptp99A^R3

fertile (with Ptp99A^R3)

Ptp99A^HA64

Other Phenotypes

Allele

abnormal neuroanatomy | adult stage (with Df(3R)Ptp99A^R3)

Ptp99A¹

chemical sensitive, with Scer\GAL4^αTub84B.PL

Ptp99A^JF01858

Phenotype manifest in

Allele

larval abdominal segmental nerve

Ptp99A¹

larval intersegmental nerve

Ptp99A¹

lobe system of adult mushroom body (with Df(3R)Ptp99A^R3)

Ptp99A¹

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (61)

Hsap\PTPRG

9 of 14

Yes

Hsap\PTPRZ1

9 of 14

Yes

Hsap\PTPN1

2 of 14

Hsap\PTPN2

2 of 14

Hsap\PTPN5

2 of 14

Yes

Hsap\PTPN7

2 of 14

Hsap\PTPN12

2 of 14

Yes

Hsap\PTPN18

2 of 14

Yes

Hsap\PTPN20

2 of 14

Yes

Hsap\PTPN22

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Hsap\PTPRR

2 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\FNDC5

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (41)

Rnor\Ptprg

9 of 14

Yes

Rnor\Ptprz1

9 of 14

Yes

Rnor\Ptpn1

2 of 14

Rnor\Ptpn7

2 of 14

Yes

Rnor\Ptpn12

2 of 14

Yes

Rnor\Ptpn18

2 of 14

Yes

Rnor\Ptpn20

2 of 14

Yes

Rnor\Ptpn22

2 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (42)

Mmus\Ptprz1

7 of 14

Yes

Mmus\Ptprg

6 of 14

Yes

Mmus\Ptpn1

2 of 14

Mmus\Ptpn2

2 of 14

Mmus\Ptpn7

2 of 14

Yes

Mmus\Ptpn12

2 of 14

Yes

Mmus\Ptpn18

2 of 14

Yes

Mmus\Ptpn20

2 of 14

Yes

Mmus\Ptpn22

2 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (22)

Xtro\ptprz1

7 of 13

Yes

Xtro\ptprg

5 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (49)

Drer\ptprz1b

10 of 14

Yes

Drer\ca16b

9 of 14

Yes

Drer\ptprga

9 of 14

Yes

Drer\ptprz1a

8 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\ptpn22

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (89)

Cele\egg-4

3 of 14

Yes

Cele\egg-5

3 of 14

Yes

Cele\Y39A3A.4

3 of 14

Yes

Cele\B0207.1

2 of 14

Yes

Cele\B0280.11

2 of 14

Yes

Cele\C02B10.6

2 of 14

Yes

Cele\C07E3.4

2 of 14

Yes

Cele\C15H7.3

2 of 14

Yes

Cele\C17H12.3

2 of 14

Yes

Cele\C17H12.5

2 of 14

Yes

Cele\C33F10.8

2 of 14

Yes

Cele\C33H5.16

2 of 14

Yes

Cele\C46A5.1

2 of 14

Yes

Cele\C55B7.3

2 of 14

Yes

Cele\egg-3

2 of 14

Yes

Cele\F20H11.4

2 of 14

Yes

Cele\F25H5.7

2 of 14

Yes

Cele\F35C11.2

2 of 14

Yes

Cele\F36H1.3

2 of 14

Cele\F36H12.10

2 of 14

Yes

Cele\F38H4.4

2 of 14

Yes

Cele\F40G12.10

2 of 14

Yes

Cele\F47B3.1

2 of 14

Yes

Cele\F47B3.2

2 of 14

Yes

Cele\F47B3.6

2 of 14

Yes

Cele\F47B3.7

2 of 14

Yes

Cele\F55F8.7

2 of 14

Yes

Cele\F57H12.5

2 of 14

Yes

Cele\K07F5.6

2 of 14

Yes

Cele\K07F5.8

2 of 14

Yes

Cele\M05B5.1

2 of 14

Yes

Cele\M05D6.3

2 of 14

Yes

Cele\pph-2

2 of 14

Yes

Cele\ptp-3

2 of 14

Cele\ptp-4

2 of 14

Yes

Cele\ptp-5.1

2 of 14

Yes

Cele\ptp-5.2

2 of 14

Yes

Cele\ptp-5.3

2 of 14

Yes

Cele\ptpn-22

2 of 14

Yes

Cele\sdf-9

2 of 14

Yes

Cele\T04F3.3

2 of 14

Yes

Cele\T13H5.1

2 of 14

Cele\T22C1.8

2 of 14

Cele\T27A3.5

2 of 14

Yes

Cele\T28F4.3

2 of 14

Yes

Cele\W01B6.6

2 of 14

Yes

Cele\Y48G9A.9

2 of 14

Yes

Cele\Y62F5A.10

2 of 14

Yes

Cele\Y71F9AL.4

2 of 14

Yes

Cele\Y116A8C.37

2 of 14

Yes

Cele\ZK354.8

2 of 14

Yes

Cele\ZK484.7

2 of 14

Yes

Cele\ZK809.1

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\eak-6

1 of 14

Cele\F10G8.1

1 of 14

Yes

Cele\F20B6.1

1 of 14

Yes

Cele\F20B6.6

1 of 14

Yes

Cele\F21C10.7

1 of 14

Cele\F55H12.5

1 of 14

Cele\hpo-7

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\R155.3

1 of 14

Yes

Cele\rig-4

1 of 14

Cele\sax-7

1 of 14

Cele\sre-45

1 of 14

Yes

Cele\T04F3.5

1 of 14

Yes

Cele\VW02B12L.2

1 of 14

Yes

Cele\Y5F2A.3

1 of 14

Yes

Cele\Y22D7AR.7

1 of 14

Yes

Cele\Y39B6A.30

1 of 14

Cele\Y48G1C.5

1 of 14

Yes

Cele\Y54F10BM.3

1 of 14

Cele\Y69A2AR.19

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\ZK616.65

1 of 14

Cele\ZK783.3

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (30)

Agam\AgaP_AGAP002832

11 of 12

Yes

Agam\AgaP_AGAP002342

5 of 12

Yes

3 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Agam\AgaP_AGAP010884

1 of 12

Agam\AgaP_AGAP011586

1 of 12

Agam\AgaP_AGAP011650

1 of 12

Arabidopsis thaliana (thale-cress) (1)

Atha\PTP1

5 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\PTP1

4 of 13

Yes

Scer\PTP3

2 of 13

Scer\PTP2

1 of 13

Schizosaccharomyces pombe (Fission yeast) (3)

Spom\pyp2

4 of 12

Yes

Spom\pyp3

3 of 12

Spom\pyp1

2 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Ptp99A. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (26)

Lar

5 of 13

5 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

2 of 13

mop

2 of 13

Ptp36E

2 of 13

PTP-ER

2 of 13

1 of 13

CG46459

1 of 13

csw

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

fra

1 of 13

IA-2

1 of 13

Idit

1 of 13

MnM

1 of 13

Pez

1 of 13

Ptpmeg

1 of 13

sdk

1 of 13

sls

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PTPRG; protein tyrosine phosphatase receptor type G

9 of 14

176886

PTPRZ1; protein tyrosine phosphatase receptor type Z1

9 of 14

176891

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Ptp99A

enzyme linked immunosorbent assay

(Ozkan et al., 2013)

Ptp99A - Abl

surface plasmon resonance, enzymatic study

(Madan et al., 2011)

Ptp99A - Csp

two hybrid

(Bugga et al., 2009)

Ptp99A - Immp2

two hybrid

(Bugga et al., 2009)

Ptp99A - InR

enzymatic study, surface plasmon resonance

(Madan et al., 2011)

Ptp99A - mys

enzymatic study, surface plasmon resonance

(Madan et al., 2011)

Ptp99A - nerfin-2

surface plasmon resonance, enzymatic study

(Madan et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

kon

suppressible

Ptp99A

(Harrison et al., 2021)

miple1

enhanceable

Ptp99A

(Muñoz-Soriano et al., 2013)

miple1

suppressible

Ptp99A

(Muñoz-Soriano et al., 2013)

nmo

enhanceable

Ptp99A

(Muñoz-Soriano et al., 2013)

Ptp69D

enhanceable

Ptp99A

(Desai et al., 1996)

Ptp69D

suppressible

Ptp99A

(Desai and Purdy, 2003)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

68335

DroID - A comprehensive database of gene and protein interactions.

FBgn0004369

MIST (genetic) - An integrated Molecular Interaction Database

43469

MIST (protein-protein) - An integrated Molecular Interaction Database

43469

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-98

Cytogenetic map

99A7-99B1

Sequence location

3R:29,377,646..29,487,131 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

99A7-99B1

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

99A7-99A8

(determined by in situ hybridisation)

(Hariharan et al., 1991)

99A7-99A7

(determined by in situ hybridisation)

(Tian et al., 1991)

99A-99A

(determined by in situ hybridisation)

(Yang et al., 1991)

Experimentally Determined Recombination Data

Location

3-98

(FlyBase, 2016)

3-96.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (123)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Korea

11974

P{VT049373-GAL4}attP2

12227

P{VT049354-GAL4}attP2

12228

P{VT049372-GAL4}attP2

12229

P{VT049377-GAL4}attP2

12785

P{VT049340-GAL4}attP2

12786

P{VT049363-GAL4}attP2

13001

P{VT049344-GAL4}attP2

13002

P{VT049356-GAL4}attP2

v103457

v103931

v108505

v200002

P{VT049348-GAL4}attP2

More stocks available...

Genomic Clones (58)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (48)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

RT09995

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0004369

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0004369

Antibody Information

Laboratory Generated Antibodies

monoclonal

(Tian et al., 1991)

polyclonal

(Hariharan et al., 1991, Yang et al., 1991)

Commercially Available Antibodies

Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research

3A6
4C71B7

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Annotation CG11515 was eliminated in release 3 of the genome annotation, October 2002.

(FlyBase Genome Annotators, 2002-2003)

Requirements of Lar, Ptp69D and Ptp99A in growth cone guidance decisions along the ISN and SNb motor pathways demonstrates relationships among the tyrosine phosphatases are complex and dependent on cellular context. At growth cone choice points along one nerve, two phosphatases cooperate, while along another nerve these same phosphatases can act in opposition to one another. Lar is central to ISN guidance, Ptp69D and Ptp99A are not essential but do participate in guidance processes involving Lar.

(Desai et al., 1997)

The products of the Ptp69D and Ptp99A genes are required for motor axon guidance and have partially redundant functions during development of the neuromuscular junction.

(Desai et al., 1996)

Spatial and temporal transcript accumulation pattern in ovaries is determined by in situ hybridisation.

(Fitzpatrick et al., 1995)

A Ptp99A cDNA has been cloned and sequenced.

(Hariharan et al., 1991)

Receptor linked PTPases may be involved in axon outgrowth and guidance during embryonic development.

(Tian et al., 1991)

Ptp99A has been characterised. Ptp99A transcripts and proteins are localised exclusively to the CNS, and expression coincides temporally with axon outgrowth.

(Yang et al., 1991)

Relationship to Other Genes

Source for database merge of

Source for merge of: Ptp99A CG2005

(Bayraktaroglu, 2000.4.13)

Source for merge of: Ptp99A CG11517 CG11516 CG11515

(FlyBase Genome Annotators, 2006)

Additional comments

Annotations CG2005, CG11517 and CG11516 merged as CG2005 (which corresponds to Ptp99A) in release 5.1 of the genome annotation. In addition, release 5.1 annotation CG2005 also contains sequences corresponding to part of release 2 annotation CG11515 (which was eliminated in release 3 of the genome annotation).

(FlyBase Genome Annotators, 2006)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

CG11515

(Luschnig, 2003.12.17)

CG11516

(Hosono et al., 2015, Khanna et al., 2010, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009.1.15, Morrison et al., 2000)

CG11517

CG2005

(Christis and Munro, 2012, Morrison et al., 2000)

CT6383

(Hynes and Zhao, 2000)

DPTP_99A

(Tian et al., 1991)

Dptp99A

(Jeong, 2021)

PTP99A

(Kawakami et al., 2022, Madan et al., 2012, Madan et al., 2011, Hofmeyer and Treisman, 2009, Andersen et al., 2004, Koushika et al., 2000)

Ptp99A

(Chen et al., 2025, Sato and Takahashi, 2025, Collins et al., 2024, Olivares et al., 2023, Beaver et al., 2022, Velten et al., 2022, Uçkun et al., 2021, Xie et al., 2021, Kurmangaliyev et al., 2020, Yanagawa et al., 2020, Hall et al., 2019, Harbison et al., 2019, Meltzer et al., 2019, Rohde et al., 2017, Shen et al., 2016, Dobson et al., 2015, Duff et al., 2015, Garlapow et al., 2015, Gene Disruption Project members, 2015-, Hatzihristidis et al., 2015, Izutsu et al., 2015, Ugrankar et al., 2015, Ashwal-Fluss et al., 2014, Sopko et al., 2014, Carter, 2013, Ghezzi et al., 2013, Muñoz-Soriano et al., 2013, Ozkan et al., 2013, Ozkan et al., 2013, Ozkan et al., 2013, Song et al., 2012, Abruzzi et al., 2011, Bellen et al., 2011, Xu et al., 2011, Wright et al., 2010, Bugga et al., 2009, Jeon et al., 2008, Kurusu and Zinn, 2008, Zhang et al., 2007, Sun et al., 2001)

R-PTP 99A

(Morrison et al., 2000)

dptp99A

(Landgraf et al., 1999, Desai et al., 1996, Stoker, 1996)

ptp99A

(Monteith et al., 2024, Harrison et al., 2021, Lin et al., 2021, Muñoz-Soriano et al., 2013)

Name Synonyms

Protein tyrosine phosphatase 99A

(Dalton et al., 2010, Khanna et al., 2010)

Secondary FlyBase IDs

FBan0002005
FBan0011517
FBgn0010251
FBgn0039661
FBgn0039662
FBgn0040621
FBgn0040622

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 68 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

43469

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P35832

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P35832

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P35832

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

43469/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004369

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004369

FlyMine - An integrated database for Drosophila genomics

FBgn0004369

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG11516

MARRVEL_MODEL - MARRVEL (model organism gene)

43469

Linkouts

BioGRID - A database of protein and genetic interactions.

68335

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0004369

DroID - A comprehensive database of gene and protein interactions.

FBgn0004369

DRSC - Results frm RNAi screens

FBgn0004369

Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research

3A6
4C71B7

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004369

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0004369

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

Flygut - An atlas of the Drosophila adult midgut

FBgn0004369

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

MIST (genetic) - An integrated Molecular Interaction Database

43469

MIST (protein-protein) - An integrated Molecular Interaction Database

43469

References (165)

The following external sites may use different assemblies or annotations than FlyBase.

-1.20

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