FlyBase Gene Report: Dmel\MAPk-Ak2

General Information

Symbol

Dmel\MAPk-Ak2

Species

D. melanogaster

Name

MAP kinase activated protein-kinase-2

Annotation Symbol

CG3086

Feature Type

protein_coding_gene

FlyBase ID

FBgn0013987

Gene Model Status

Current

Stock Availability

12 publicly available

Enzyme Name (EC)

calcium/calmodulin-dependent protein kinase (2.7.11.17)

Gene Summary

Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25). (UniProt, P49071)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

MK2, dMK2

Key Links

Genomic Location

Cytogenetic map

5B8-5B8

Sequence location

X:5,759,908..5,764,203 [-]

Recombination map

1-13

RefSeq locus

NC_004354 REGION:5759908..5764203

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (13 terms)

Molecular Function (6 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein kinase activity

inferred from direct assay

(Tian et al., 2014)

Terms Based on Predictions or Assertions (6 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR017441

(InterPro Project Members, 2004-)

enables calcium-dependent protein serine/threonine kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN000681419

(GO Reference Genome Project, 2011-)

enables calcium/calmodulin-dependent protein kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN000681419

(GO Reference Genome Project, 2011-)

enables calmodulin binding

inferred from biological aspect of ancestor with PANTHER:PTN000681419

(GO Reference Genome Project, 2011-)

enables mitogen-activated protein kinase binding

inferred from biological aspect of ancestor with PANTHER:PTN001218849

(GO Reference Genome Project, 2011-)

enables protein kinase activity

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR008271

(InterPro Project Members, 2004-)

Biological Process (5 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in negative regulation of mitochondrial electron transport, NADH to ubiquinone

inferred from genetic interaction with FLYBASE:IscU; FB:FBgn0037637

(Tian et al., 2014)

involved_in positive regulation of cell size

inferred from genetic interaction with FLYBASE:gig; FB:FBgn0005198

(Cully et al., 2010)

involved_in regulation of JNK cascade

inferred from genetic interaction with FLYBASE:bsk; FB:FBgn0000229

(Seisenbacher et al., 2011)

inferred from genetic interaction with FLYBASE:Mkk4; FB:FBgn0024326

(Seisenbacher et al., 2011)

inferred from genetic interaction with FLYBASE:Tak1; FB:FBgn0026323

(Seisenbacher et al., 2011)

involved_in response to salt stress

inferred from mutant phenotype

(Seisenbacher et al., 2011)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in intracellular signal transduction

inferred from biological aspect of ancestor with PANTHER:PTN000681419

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000681419

(GO Reference Genome Project, 2011-)

located_in cytoplasm

inferred from sequence or structural similarity with UniProtKB:P49137

(Gene Ontology Curators, 2002-)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000681419

(GO Reference Genome Project, 2011-)

located_in nucleus

inferred from sequence or structural similarity with UniProtKB:P49137

(Gene Ontology Curators, 2002-)

Gene Group (FlyBase)

CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASES

Protein Family (UniProt)

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. (P49071)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

calcium/calmodulin-dependent protein kinase activity

(1) L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+) (2.7.11.17)

(2) L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+) (2.7.11.17)

Summaries

Gene Group (FlyBase)

CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASES -: The Calcium/Calmodulin-dependent kinase family are serine/threonine kinases which, in general, prefer substrates containing basic residues. Many, but not all, members are regulated by calcium or calmodulin. (Adapted from FBrf0132098).

Protein Function (UniProtKB)

Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25).

(UniProt, P49071)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\MAPk-Ak2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P49071)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.52

Gene model reviewed during 5.39

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.49

Gene model reviewed during 5.55

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

MAPk-Ak2-RB

FBtr0070837

NM_080030

2020

359

MAPk-Ak2-RD

FBtr0070839

NM_176688

2045

359

MAPk-Ak2-RE

FBtr0302940

NM_001201618

2060

359

MAPk-Ak2-RF

FBtr0308577

NM_001258607

1913

359

Additional Transcript Data and Comments

Reported size (kB)

2.4, 2.3, 1.8 (northern blot)

(Larochelle and Suter, 1995)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

MAPk-Ak2-PB

FBpp0070802

41.4

359

7.38

FBpp0070804

41.4

359

7.38

FBpp0292066

41.4

359

7.38

FBpp0300801

41.4

359

7.38

P49071

NP_001245536

AFH07250

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

359 aa isoforms: MAPk-Ak2-PB, MAPk-Ak2-PD, MAPk-Ak2-PE, MAPk-Ak2-PF

Additional Polypeptide Data and Comments

Reported size (kDa)

359 (aa); 42 (kD predicted)

(Larochelle and Suter, 1995)

Comments

External Data

Post Translational Modification

Phosphorylated and activated by MAP kinase.

(UniProt, P49071)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\MAPk-Ak2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

MAPk-Ak2_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.67

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis & adult stage | female

ovary

(Larochelle and Suter, 1995)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Larochelle and Suter, 1995)

embryonic stage 1 -- 9

organism

(Larochelle and Suter, 1995)

oogenesis & adult stage | female

ovary

(Larochelle and Suter, 1995)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\MAPk-Ak2 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\MAPk-Ak2

View exonic expression by tissue for Dmel\MAPk-Ak2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

44573/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0013987

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0013987

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0013987

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3086

Flygut - An atlas of the Drosophila adult midgut

FBgn0013987

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 8 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 16 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of MAPk-Ak2

Transgenic constructs containing regulatory region of MAPk-Ak2

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(1)Exel6236

(Ryder, 2004.4.26)

Inferred from location ( 21 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal - majority live | nutrition conditional

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

MAPk-Ak2^GD1597

viable, with Scer\GAL4^Mef2.PR

MAPk-Ak2^GD1597

viable, with Scer\GAL4^pnr-MD237

MAPk-Ak2^GD1597

viable, with Scer\GAL4^tin.CΔ4

MAPk-Ak2^GD1597

Sterility

Allele

female semi-sterile, with Scer\GAL4^{VP16.mat.αTub67C}

female semi-sterile, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

MAPk-Ak2^GL00223

Other Phenotypes

Allele

abnormal oxidative stress response

MAPk-Ak2^5B

melanotic mass phenotype | larval stage | nutrition conditional

Phenotype manifest in

Allele

embryonic/larval hindgut | larval stage | posterior | nutrition conditional

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (26)

Hsap\MAPKAPK3

14 of 14

Yes

Hsap\MAPKAPK2

12 of 14

Yes

Hsap\MAPKAPK5

5 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (14)

Rnor\Mapkapk3

14 of 14

Yes

Rnor\Mapkapk2

11 of 14

Yes

Rnor\Mapkapk5

4 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (13)

Mmus\Mapkapk3

14 of 14

Yes

Mmus\Mapkapk2

11 of 14

Yes

Mmus\Mapkapk5

5 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (14)

Xtro\mapkapk2

7 of 13

Yes

Xtro\mapkapk3

7 of 13

Yes

Xtro\crnkl1

6 of 13

Xtro\mapkapk5

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (20)

Drer\mapkapk3

13 of 14

Yes

Drer\mapkapk2a

12 of 14

Yes

Drer\mapkapk2b

11 of 14

Yes

Drer\mapkapk5

5 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (11)

Cele\mak-2

13 of 14

Yes

Cele\mak-1

3 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (4)

Agam\AgaP_AGAP011890

7 of 12

Yes

Agam\AgaP_AGAP002469

1 of 12

Agam\AgaP_AGAP003715

1 of 12

Agam\AgaP_AGAP005306

1 of 12

Arabidopsis thaliana (thale-cress) (48)

Atha\CDPK2

2 of 13

Yes

Atha\CDPK6

2 of 13

Yes

Atha\CDPK9

2 of 13

Yes

Atha\CDPK19

2 of 13

Yes

Atha\CPK1

2 of 13

Yes

Atha\CPK2

2 of 13

Yes

Atha\CPK4

2 of 13

Yes

Atha\CPK7

2 of 13

Yes

Atha\CPK15

2 of 13

Yes

Atha\CPK17

2 of 13

Yes

Atha\CPK20

2 of 13

Yes

Atha\CPK25

2 of 13

Yes

Atha\CPK34

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (5)

Scer\AKL1

1 of 13

Yes

Scer\ARK1

1 of 13

Yes

Scer\ENV7

1 of 13

Yes

Scer\NNK1

1 of 13

Yes

Scer\PRK1

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:MAPk-Ak2. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (8)

Lk6

3 of 13

CG17528

2 of 13

aux

1 of 13

CaMKI

1 of 13

CaMKII

1 of 13

CG1227

1 of 13

Nak

1 of 13

png

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

MAPKAPK3; MAPK activated protein kinase 3

model of patterned macular dystrophy 3

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

MAPKAPK3; MAPK activated protein kinase 3

14 of 14

602130

MACULAR DYSTROPHY, PATTERNED, 3; MDPT3

patterned macular dystrophy 3

MAPKAPK2; MAPK activated protein kinase 2

12 of 14

602006

MAPKAPK5; MAPK activated protein kinase 5

5 of 14

606723

NEUROCARDIOFACIODIGITAL SYNDROME; NCFD

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Dmel gene

Ortholog showing functional complementation

Supporting References

MAPk-Ak2

Mmus\Mapkapk2

(Seisenbacher et al., 2011)

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

MAPk-Ak2 - IscU

anti tag coimmunoprecipitation, anti tag western blot, enzymatic study, autoradiography

(Tian et al., 2014)

MAPk-Ak2 - p38a

experimental knowledge based

(Guruharsha et al., 2011)

MAPk-Ak2 - p38b

anti tag coimmunoprecipitation, peptide massfingerprinting, anti tag western blot, pull down, western blot, experimental knowledge based

(Belozerov et al., 2012, Guruharsha et al., 2011, Seisenbacher et al., 2011)

MAPk-Ak2 - ph-p

anti tag coimmunoprecipitation, anti tag western blot

(Prickaerts et al., 2012)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

MAPk-Ak2

suppressible

bsk

(Seisenbacher et al., 2011)

MAPk-Ak2

suppressible

Mkk4

(Seisenbacher et al., 2011)

MAPk-Ak2

suppressible

Tak1

(Seisenbacher et al., 2011)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

p38a

enhanceable

MAPk-Ak2

(Seisenbacher et al., 2011)

p38b

enhanceable

MAPk-Ak2

(Seisenbacher et al., 2011)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

69137

DroID - A comprehensive database of gene and protein interactions.

FBgn0013987

MIST (protein-protein) - An integrated Molecular Interaction Database

44573

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Efferocytosis

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

P49071

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-13

Cytogenetic map

5B8-5B8

Sequence location

X:5,759,908..5,764,203 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

5B8-5B8

Limits computationally determined from genome sequence between P{EP}EP404&P{EP}EP1405 and P{EP}CG15770^EP390

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

5D-5D

(Larochelle, 1995.2.6)

5D-5D

(determined by in situ hybridisation)

(Larochelle and Suter, 1995)

Experimentally Determined Recombination Data

Location

1-13

(FlyBase, 2016)

1-14.9

(Comeron et al., 2012)

(Larochelle, 1995.2.6)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (12)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

33524

w^* P{EP}MAPk-Ak2^G265

63717

y¹ v¹; P{TRiP.HMJ30285}attP40

602804

y¹ v¹; P{CFD4FLPOUT-Lk6-MAPk-Ak2}attP40/CyO

35317

y¹ sc^* v¹ sev²¹; P{TRiP.GL00223}attP2

41894

y¹ sc^* v¹ sev²¹; P{TRiP.GL01326}attP2

57013

y¹ sc^* v¹ sev²¹; P{TRiP.HMS04456}attP40

Korea

10627

P{EP}MAPk-Ak2^G265

NIG-Fly

HMJ30285

y¹ v¹; P{TRiP.HMJ30285}attP40/CyO

GL00223

y¹ sc^* v¹ sev²¹; P{TRiP.GL00223}attP2

VDRC

v110317

P{KK100594}VIE-260B

v3170

w¹¹¹⁸; P{GD1597}v3170

v342009

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD01359}attP40/CyO-GFP

Genomic Clones (15)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (77)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD26767

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0013987

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0013987

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Seisenbacher et al., 2011)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, spindle abnormalities and chromosome alignment defects are seen.

(Bettencourt-Dias et al., 2004)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in S2R⁺ cells: cells become round and detached. Kc167 cells are unaffected.

(Kiger et al., 2003)

An evolutionarily conserved MAP kinase-like pathway that responds to stress but not to mitogenic signals exists, MAPk-Ak2 is part of this stress-responsive pathway.

(Larochelle and Suter, 1995)

Comparison of sequence with the mammalian counterpart reveals the catalytic domain, MAPK phosphorylation site and the nuclear targeting sequence are conserved.

(Larochelle and Suter, 1995)

Relationship to Other Genes

Source for database merge of

Source for merge of: MAPk-Ak2 CG3086

(Bayraktaroglu, 2000.4.12)

Additional comments

FlyBase curator comment: Correspondence with author Seisenbacher confirmed that 'MK2'/'MAPKAPK-2' = 'CG3086'/'FBgn0013987'.

(Gerhard et al., 2007)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

CG3086

(Schertel et al., 2013, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Li et al., 2007, Morrison et al., 2000)

DmMAPKAPK-2

(Larochelle and Suter, 1995)

MAPKAP

(Morrison et al., 2000)

MAPKAPK-2

(Gerhard et al., 2007)

MAPk-Ak2

(Port et al., 2020, Myat et al., 2019, Kanoh et al., 2015, Ashwal-Fluss et al., 2014, Sopko et al., 2014, Bonke et al., 2013, Schertel et al., 2013, Hazelett et al., 2012, Li et al., 2007, Loop et al., 2004)

MK2

(Maier et al., 2019, Terriente-Félix et al., 2017, Belozerov et al., 2014, Seisenbacher et al., 2011, Cully et al., 2010, Seisenbacher et al., 2008, Gerhard et al., 2007)

dMK2

(Tian et al., 2014, Chen et al., 2010)

Name Synonyms

MAP kinase activated protein kinase-2

(Larochelle, 1995.2.6)

MAP kinase activated protein-kinase-2

MAP kinase-activated protein kinase 2

(Castillo et al., 2015)

Secondary FlyBase IDs

FBgn0029802

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 59 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

44573

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P49071

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P49071

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P49071

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

44573/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0013987

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0013987

FlyMine - An integrated database for Drosophila genomics

FBgn0013987

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG3086

MARRVEL_MODEL - MARRVEL (model organism gene)

44573

Linkouts

BioGRID - A database of protein and genetic interactions.

69137

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0013987

DroID - A comprehensive database of gene and protein interactions.

FBgn0013987

DRSC - Results frm RNAi screens

FBgn0013987

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

MAPk-Ak2_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0013987

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0013987

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3086

Flygut - An atlas of the Drosophila adult midgut

FBgn0013987

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0013987

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC013565

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Efferocytosis

MIST (protein-protein) - An integrated Molecular Interaction Database

44573

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

P49071

References (72)

The following external sites may use different assemblies or annotations than FlyBase.

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