FlyBase Gene Report: Dmel\emb

General Information

Symbol

Dmel\emb

Species

D. melanogaster

Name

embargoed

Annotation Symbol

CG13387

Feature Type

protein_coding_gene

FlyBase ID

FBgn0020497

Gene Model Status

Current

Stock Availability

14 publicly available

Gene Summary

embargoed (emb) encodes an exportin involved in protein export from the nucleus. It regulates the response to hypoxia and is involved in centriole replication. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

Crm1, dCRM1, XPO1, Exportin, l(2)k16715

Key Links

Genomic Location

Cytogenetic map

29C1-29C3

Sequence location

2L:8,403,573..8,408,853 [+]

Recombination map

2-32

RefSeq locus

NT_033779 REGION:8403573..8408853

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (16 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables nuclear export signal receptor activity

inferred from mutant phenotype

(Xylourgidis et al., 2006)

inferred from direct assay

(Roth et al., 2003)

enables protein binding

inferred from physical interaction with FLYBASE:Nup214; FB:FBgn0010660

(Xylourgidis et al., 2006)

inferred from physical interaction with UniProtKB:Q9GYU8

(Roth et al., 2003)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables nuclear export signal receptor activity

inferred from electronic annotation with InterPro:IPR014877, InterPro:IPR045065

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000131749

(GO Reference Genome Project, 2011-)

enables small GTPase binding

inferred from electronic annotation with InterPro:IPR001494

(InterPro Project Members, 2004-)

Biological Process (9 terms)

Terms Based on Experimental Evidence (7 terms)

CV Term

Evidence

References

involved_in centriole replication

inferred from mutant phenotype

(Dobbelaere et al., 2008)

involved_in nuclear export

inferred from direct assay

(Song et al., 2005)

involved_in nuclear pore complex assembly

inferred from mutant phenotype

(Hampoelz et al., 2019)

involved_in protein export from nucleus

inferred from mutant phenotype

(Irisarri et al., 2009, Bi et al., 2005)

inferred from mutant phenotype

(Collier et al., 2000)

involved_in protein transport

inferred from direct assay

(Roth et al., 2003)

involved_in regulation of cellular response to hypoxia

inferred from mutant phenotype

(Irisarri et al., 2009)

involved_in ribosomal small subunit export from nucleus

inferred from mutant phenotype

(Kim et al., 2015)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in intracellular protein transport

inferred from electronic annotation with InterPro:IPR001494

(InterPro Project Members, 2004-)

involved_in protein export from nucleus

inferred from electronic annotation with InterPro:IPR045065

(InterPro Project Members, 2004-)

involved_in ribosomal large subunit export from nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000131787

(GO Reference Genome Project, 2011-)

involved_in ribosomal small subunit export from nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000131787

(GO Reference Genome Project, 2011-)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in nuclear membrane

inferred from direct assay

(Dobbelaere et al., 2008)

part_of nuclear pore

inferred from mutant phenotype

(Roth et al., 2003)

located_in nucleus

inferred from direct assay

(Dobbelaere et al., 2008)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000131749

(GO Reference Genome Project, 2011-)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000131749

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

BETA IMPORTINS

Protein Family (UniProt)

Belongs to the exportin family. (Q9TVM2)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

embargoed (emb) encodes an exportin involved in protein export from the nucleus. It regulates the response to hypoxia and is involved in centriole replication. [Date last reviewed: 2019-03-07]

Gene Group (FlyBase)

BETA IMPORTINS -: Importin-β family proteins are nucleocytoplasmic transport receptors that import and export proteins and RNAs through the nuclear pores. In some import/export pathways, importin-β directly interacts with the cargo. For cargo containing a classical nuclear localization (cNLS) signal, importin-α acts as an adaptor linking the cargo to importin-β. (Adapted from FBrf0235243, FBrf0131350 and PMID:21029754).

Protein Function (UniProtKB)

Receptor for the leucine-rich nuclear export signal (NES) (PubMed:10636888, PubMed:14638854, PubMed:16103875). Binds cooperatively to the NES on its target protein and to the small GTPase Ran in its active GTP-bound form (PubMed:10636888, PubMed:14638854). Involved in the export of dl, RpS2 and the pre-40S ribosome from the nucleus to the cytoplasm (PubMed:14638854, PubMed:17032737, PubMed:25858587). Plays an important role in nuclear pore assembly by mediating nucleoporin condensation and biogenesis of annulate lamellae (PubMed:31626769). Required for the function or maintenance of certain tissues such as brain and gut (PubMed:10924475).

(UniProt, Q9TVM2)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\emb for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9TVM2)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.50

Gene model reviewed during 5.45

Gene model reviewed during 6.02

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

emb-RA

FBtr0079663

NM_164818

4492

1063

emb-RB

FBtr0346708

NM_001316387

3919

1063

Additional Transcript Data and Comments

Reported size (kB)

4.2, 3.5 (northern blot)

(Collier et al., 2000)

3.8 (northern blot)

(Fasken et al., 2000)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

emb-PA

FBpp0079278

122.8

1063

5.70

FBpp0312321

122.8

1063

5.70

Q9TVM2

NP_001303316

ALI30202

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1063 aa isoforms: emb-PA, emb-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

1063 (aa)

(Collier et al., 2000)

1063 (aa); 123 (kD predicted)

(Fasken et al., 2000)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with Clbn (via its N-terminus) (PubMed:16103875). Associates with the nuclear pore complex via interaction with mbo and Nup214 (PubMed:14638854, PubMed:17032737). Interacts with target proteins containing NES sequences such as actin and dl (PubMed:10924475, PubMed:14638854, PubMed:17032737).

(UniProt, Q9TVM2)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\emb using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.96

Transcript Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic/larval midgut

(Collier et al., 2000)

embryonic/larval posterior spiracle

(Collier et al., 2000)

embryonic brain

(Collier et al., 2000)

organism | ubiquitous

(Collier et al., 2000)

larval somatic muscle cell

(Collier et al., 2000)

larval ventral nerve cord

(Collier et al., 2000)

embryonic/larval hindgut

(Collier et al., 2000)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

anterior endoderm anlage

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 13 -- 15

embryonic brain

(Collier et al., 2000)

embryonic hindgut

(Collier et al., 2000)

posterior spiracle primordium

(Collier et al., 2000)

embryonic stage 15

larval ventral nerve cord

(Collier et al., 2000)

embryonic/larval midgut primordium

(Collier et al., 2000)

embryonic somatic muscle cell

(Collier et al., 2000)

Additional Descriptive Data

emb transcripts are expressed ubiquitously at all stages of embryonic development. Elevated levels are observed in brain, hindgut, and posterior spiracles shortly before dorsal closure and in ventral nerve cord, midgut, and somatic musculature after dorsal closure.

(Collier et al., 2000)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in nuclear membrane

inferred from direct assay

(Dobbelaere et al., 2008)

part_of nuclear pore

inferred from mutant phenotype

(Roth et al., 2003)

located_in nucleus

inferred from direct assay

(Dobbelaere et al., 2008)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\emb in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\emb

View exonic expression by tissue for Dmel\emb

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0020497

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34167/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0020497

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0020497

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0020497

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG13387

Flygut - An atlas of the Drosophila adult midgut

FBgn0020497

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\emb

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 11-12

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 17 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 12 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of emb

Transgenic constructs containing regulatory region of emb

Aberrations (Deficiencies and Duplications) ( 5 )

Inferred from experimentation ( 5 )

Gene disrupted in

Df(2L)Exel8021

(Ryder, 2004.4.26)

Df(2L)N22-14

(Collier et al., 2000, Spradling et al., 1999, BDGP Project Members, 1994-1999)

T(Y;2)fy⁴

(Collier et al., 2000)

Gene not disrupted in

Df(2L)N22-5

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(2L)TE29Aa-11

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal (with Df(2L)N22-14)

lethal | recessive

lethal - all die before end of larval stage, with Scer\GAL4^phtm.PO

emb^GD1239

lethal - all die before end of larval stage (with Df(2L)N22-14)

lethal - all die before end of second instar larval stage

emb²

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

emb^GD1239

partially lethal - majority die, with Scer\GAL4^pnr-MD237

emb^GD1239

viable, with Scer\GAL4^tin.CΔ4

emb^GD1239

Other Phenotypes

Allele

abnormal developmental rate, with Scer\GAL4^phtm.PO

emb^GD1239

abnormal stress response | embryonic stage

emb³

abnormal stress response | embryonic stage, with Scer\GAL4^en-e16E

emb^UAS.cIa

increased body size | larval stage, with Scer\GAL4^phtm.PO

emb^GD1239

some die during larval stage (with Df(2L)N22-14)

some die during pupal stage, with Scer\GAL4^pnr-MD237

emb^GD1239

some die during second instar larval stage

emb²

Phenotype manifest in

Allele

annulate lamellae | oogenesis, with Scer\GAL4^{VP16.nanos.UTR}

emb^HMS00991

chromatin | larval stage, with Scer\GAL4^phtm.PO

emb^GD1239

larval tracheal system

emb^unspecified

oocyte, with Scer\GAL4^{VP16.nanos.UTR}

emb^HMS00991

prothoracic gland | larval stage, with Scer\GAL4^phtm.PO

emb^GD1239

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (6)

Hsap\XPO1

14 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (6)

Rnor\Xpo1

14 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mus musculus (laboratory mouse) (6)

Mmus\Xpo1

13 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (7)

Xtro\xpo1

11 of 13

Yes

Xtro\abhd5

1 of 13

Xtro\LOC100486598

1 of 13

Yes

Xtro\uspl1

1 of 13

Yes

Xtro\xpo5.2

1 of 13

Yes

Xtro\xpo5

1 of 13

Xtro\xpot

1 of 13

Yes

Danio rerio (Zebrafish) (7)

Drer\xpo1a

13 of 14

Yes

Drer\xpo1b

13 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (4)

Cele\xpo-1

13 of 14

Yes

Cele\T16G12.6

1 of 14

Cele\tsr-1

1 of 14

Cele\xpo-3

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (5)

Agam\AgaP_AGAP009929

12 of 12

Yes

2 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (6)

Atha\XPO1A

13 of 13

Yes

Atha\XPO1B

13 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (4)

Scer\CRM1

13 of 13

Yes

Scer\LOS1

1 of 13

Yes

Scer\MSN5

1 of 13

Scer\MTR10

1 of 13

Schizosaccharomyces pombe (Fission yeast) (5)

Spom\crm1

12 of 12

Yes

Spom\kap111

1 of 12

Spom\los1

1 of 12

Yes

Spom\mtr10

1 of 12

Spom\SPAC328.01c

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:emb. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (5)

Ranbp21

4 of 13

cdm

1 of 13

CG10950

1 of 13

ebo

1 of 13

Tnpo-SR

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 6 )

Allele

Disease

Interaction

References

emb^JF01311

exacerbates amyotrophic lateral sclerosis

modeled by Zzzz\GGGGCC^58.UAS.EGFP

(Freibaum et al., 2015)

exacerbates frontotemporal dementia

modeled by Zzzz\GGGGCC^58.UAS.EGFP

(Freibaum et al., 2015)

exacerbates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Sowa et al., 2018)

ameliorates C9orf72 frontotemporal dementia and/or amyotrophic lateral sclerosis

modeled by Zzzz\GGGGCC^30.UAS.EGFP

(Cunningham et al., 2020)

modeled by Zzzz\poly-PR^{25.UAS.Tag:FLAG}

(Boeynaems et al., 2016)

emb^NIG.13387R

exacerbates Machado-Joseph disease

modeled by Hsap\ATXN3^{fl.Q84.UAS.Tag:MYC}

(Chan et al., 2011)

emb^E128-1A

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Bilen and Bonini, 2007)

modeled by Hsap\ATXN3^{fl.Q84.UAS.Tag:MYC}

(Chan et al., 2011)

emb^EY08770

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Sowa et al., 2018)

emb^E2-1A

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Bilen and Bonini, 2007)

emb^KK102552

exacerbates acute myeloid leukemia

modeled by Hsap\HOXA9::Hsap\NUP98^WT.UAS

(Gavory et al., 2021)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

XPO1; exportin 1

14 of 14

602559

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

emb - Cand1

experimental knowledge based

(Guruharsha et al., 2011)

emb - Dfd

bimolecular fluorescence complementation, fluorescence microscopy

(Duffraisse et al., 2020)

emb - Ipo9

experimental knowledge based

(Guruharsha et al., 2011)

emb - Karyβ3

experimental knowledge based

(Guruharsha et al., 2011)

emb - MRP

experimental knowledge based

(Guruharsha et al., 2011)

emb - Nup214

pull down, anti tag western blot, two hybrid, anti bait coimmunoprecipitation, western blot

(Xylourgidis et al., 2006)

emb - Pp2A-29B

experimental knowledge based

(Guruharsha et al., 2011)

emb - Rdh1

experimental knowledge based

(Guruharsha et al., 2011)

emb - Scamp

experimental knowledge based

(Guruharsha et al., 2011)

emb - Scr

pull down, western blot, bimolecular fluorescence complementation, fluorescence microscopy

(Duffraisse et al., 2020)

emb - Sec61α

experimental knowledge based

(Guruharsha et al., 2011)

emb - Ubx

bimolecular fluorescence complementation, fluorescence microscopy, pull down, western blot

(Duffraisse et al., 2020)

emb - Z600

anti bait coimmunoprecipitation, peptide massfingerprinting

(Gawlinski et al., 2007)

emb - fra

anti tag coimmunoprecipitation, anti tag western blot, western blot

(Zang and Bashaw, 2023)

emb - tim

anti tag coimmunoprecipitation, anti tag western blot, pull down, western blot

(Cai et al., 2021)

RNA-protein

Physical Interaction

Assay

References

emb - polo

pull down, Identification by mass spectrometry

(Oliveira et al., 2019)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

emb

suppressible

mbo

(Roth et al., 2003)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

hid

enhanceable

emb

(Bilen and Bonini, 2007)

External Data

Subunit Structure (UniProtKB)

(UniProt, Q9TVM2 )

Linkouts

BioGRID - A database of protein and genetic interactions.

60290

DroID - A comprehensive database of gene and protein interactions.

FBgn0020497

MIST (protein-protein) - An integrated Molecular Interaction Database

34167

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-32

Cytogenetic map

29C1-29C3

Sequence location

2L:8,403,573..8,408,853 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

29C1-29C3

Limits computationally determined from genome sequence between P{lacW}l(2)k12914^k12914&P{lacW}Akap200^k07118a and P{lacW}l(2)k03706^k03706&P{lacW}Acer^k07704

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

29C-29C

(Hovemann, 1999.8.27)

29C1-29C5

(Fasken, 1999.9.28)

29C1-29C2

(Collier, 1999.8.23)

29C3-29C4

(determined by in situ hybridisation)

(Spradling et al., 1999)

29C3-29C4

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

2-32

(FlyBase, 2016)

2-27.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (14)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

31353

y¹ v¹; P{TRiP.JF01311}attP2

81489

y¹ v¹; P{TKO.GS04819}attP40

11195

y¹ w^67c23; P{lacW}emb^k16715/CyO

19935

y¹ w^67c23; P{EPgy2}emb^EY08770/CyO

602843

y¹ w^*; TI{CRIMIC.TG4.1}emb^{CR71266-TG4.1}/SM6a

34021

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00991}attP2

Kyoto

102459

y¹ w^67c23 ; P{lacW}emb^k06303 / CyO

NIG-Fly

13387R-1

P{NIG.13387R}1

13387R-4

P{NIG.13387R}4

HMS00991

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00991}attP2

VDRC

v103767

P{KK102552}VIE-260B

v3347

w¹¹¹⁸; P{GD1239}v3347

More stocks available...

Genomic Clones (21)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (112)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

DPiM_LD45706

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0020497

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0020497

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

sima protein nuclear export is affected in emb mutant embryos.

(Irisarri et al., 2009)

emb is involved in centriole duplication.

(Dobbelaere et al., 2008)

Mutations of the S.pombe crm1 are suppressed by expression of the Drosophila emb.

(Collier et al., 1997)

Relationship to Other Genes

Source for database merge of

Source for merge of: emb l(2)k16715

(Collier et al., 2000)

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

The gene is named "embargoed" to reflect the nuclear export defects of the mutants.

(Collier et al., 2000)

Synonyms and Secondary IDs (24)

Reported As

Symbol Synonym

CG13387

(National Institute of Genetics Fly Stocks, 2022-, Metwally et al., 2019, Hosono et al., 2015, Ding et al., 2014, Schertel et al., 2013, Christis and Munro, 2012, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Mason and Goldfarb, 2009, Dobbelaere et al., 2008, Ni et al., 2008, Lippai et al., 2000)

CRM-1

(van Soldt and Cardoso, 2020)

CRM1

(Gamez et al., 2020, Irisarri et al., 2009, Sabri et al., 2007, Xylourgidis et al., 2006, Tootle and Rebay, 2005, Herold et al., 2003, Roth et al., 2003, Roth et al., 2003, Roth et al., 2003, Xylourgidis et al., 2003, Bienz, 2002, Fasken, 1999.9.28)

Crm1

(Ho et al., 2024, Wang et al., 2021, Kneuss et al., 2019, Freibaum et al., 2015, Kim et al., 2015, Büssing et al., 2010, Gawlinski et al., 2007, Song et al., 2005, Bono et al., 2004, Hieronymus and Silver, 2004, Hovemann, 1999.8.27, Collier and Gubb, 1997, Collier et al., 1997)

DCRM1

(Roth et al., 2003)

EN2-1

(Gavory et al., 2021)

Emb

(Zang and Bashaw, 2023, Keegan and Hughes, 2021, Duffraisse et al., 2020, Metwally et al., 2019, Oliveira et al., 2019, Sowa et al., 2018, Boeynaems et al., 2016, Schwartz et al., 2016, Finan et al., 2011, Büssing et al., 2010)

XPO-1

(Büssing et al., 2010)

XPO1

(Layalle et al., 2021, Swevers et al., 2018, Büssing et al., 2010)

Xpo1

(Liguori et al., 2021, Chan et al., 2011)

crm1

(Nawathean and Rosbash, 2004, Tootle et al., 2003)

dCRM1

(Jin et al., 2012, Roth et al., 2003, Xylourgidis et al., 2003, Fasken et al., 2000)

dXPO1

(Yasunaga et al., 2014)

l(2)k16715

(Spradling et al., 1999)

xpo1

(Cai et al., 2021)

Name Synonyms

Chromosomal region maintenance 1

(Collier et al., 1997)

Embargoed

(Deivasigamani et al., 2014, Büssing et al., 2010)

Enhancer of NA9 2-1

(Gavory et al., 2021)

Exportin

(Zhang et al., 2015, Bi et al., 2005, Bi et al., 2004)

Exportin-1

(Nie et al., 2009)

embargoed

(Hampoelz et al., 2019, Akan et al., 2016, Kimura and Imamoto, 2014, Yasunaga et al., 2014, Irisarri et al., 2009, Mason and Goldfarb, 2009, Bilen and Bonini, 2007, Bi et al., 2005, Roth et al., 2003)

exportin

(Jin et al., 2012)

exportin-1

(Chan et al., 2011)

Secondary FlyBase IDs

FBgn0021807

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 69 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34167

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9TVM2

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9TVM2

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9TVM2

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0020497

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34167/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0020497

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0020497

FlyMine - An integrated database for Drosophila genomics

FBgn0020497

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG13387

MARRVEL_MODEL - MARRVEL (model organism gene)

34167

Linkouts

BioGRID - A database of protein and genetic interactions.

60290

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0020497

DroID - A comprehensive database of gene and protein interactions.

FBgn0020497

DRSC - Results frm RNAi screens

FBgn0020497

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0020497

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0020497

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG13387

Flygut - An atlas of the Drosophila adult midgut

FBgn0020497

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0020497

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC012162

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (protein-protein) - An integrated Molecular Interaction Database

34167

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (131)

The following external sites may use different assemblies or annotations than FlyBase.

-0.96

Report Sections