guf, gut feeling, l(2)90/37
Please see the JBrowse view of Dmel\SmD3 for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.39
Gene model reviewed during 5.49
0.95 (northern blot)
There is only one protein coding transcript and one polypeptide associated with this gene
151 (aa); 16 (kD predicted)
Interacts with the SMN complex.
Methylated on arginine residues by Art5 and Art7; methylation is not required for assembly and biogenesis of snRNPs.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\SmD3 using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
SmD3 transcripts are detected in embryonic RNA.
JBrowse - Visual display of RNA-Seq signals
View Dmel\SmD3 in JBrowsePlease Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
RNAi screen using dsRNA made from templates generated with primers directed against this gene results in a long metaphase spindle with misaligned chromosomes when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
Mutants isolated in a screen of the second chromosome identifying genes affecting disc morphology.
SmD3 plays a role in the development of the embryonic peripheral nervous system and is required for normal development of the somatic musculature.
Mutants display a brain, imaginal disc and blood cell neoplastic phenotype.
Genetic and developmental characteristics of the lymph gland and brain overgrowth mutant have been determined.
The mutant phenotypes of the "l(2)k09540" and "l(2)k11803" lines were previously attributed to an effect on the Oda gene (FBrf0089808), however, evidence in FBrf0149027 shows that the mutant phenotype of these lines (and other P-element insertion lines located within an intron of Oda) is actually due to an effect on SmD3 (a gene nested in an intron of Oda) and not on Oda.
The complementation group represented by "l(2)k11803", "l(2)k09540" and "l(2)k09512" is named "gutfeeling" because all the P{lacW} insertion mutants confer strong Ecol\lacZ expression in the gut and affect sensory organ development. FlyBase curator comment: the mutant phenotype of "l(2)k11803", "l(2)k09540" and "l(2)k09512" is attributed to an effect on the Oda gene in FBrf0089808 and the Oda gene is shown to be expressed in the gut in FBrf0089808. However, FBrf0149027 shows that the mutant phenotype of "l(2)k11803", "l(2)k09540" and "l(2)k09512" is actually due to an effect on SmD3 (a gene nested in an intron of Oda) and not on Oda.
