FlyBase Gene Report: Dmel\NC2β

General Information

Symbol

Dmel\NC2β

Species

D. melanogaster

Name

Negative Cofactor 2β

Annotation Symbol

CG4185

Feature Type

protein_coding_gene

FlyBase ID

FBgn0028926

Gene Model Status

Current

Stock Availability

8 publicly available

Gene Summary

Bifunctional basic transcription factor. Activates transcription of DPE (Downstream Promoter Element) containing promoters while repressing transcription of promoters which contain TATA elements (PubMed:11062130). Together with Chrac-14, promotes nucleosome sliding of ATP-dependent nucelosome remodeling complexes (PubMed:18327268). (UniProt, Q9VJQ5)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

BG:DS00929.3 , Dr1, NC2 beta

Key Links

Genomic Location

Cytogenetic map

35B8-35B8

Sequence location

2L:15,007,813..15,008,538 [+]

Recombination map

2-50

RefSeq locus

NT_033779 REGION:15007813..15008538

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (17 terms)

Molecular Function (6 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables core promoter sequence-specific DNA binding

inferred from direct assay

(Willy et al., 2000)

enables protein binding

inferred from physical interaction with UniProtKB:Q9W2E3

(assigned by UniProt )

(Willy et al., 2000)

enables transcription factor binding

inferred from physical interaction with FLYBASE:NC2α; FB:FBgn0034650

(Willy et al., 2000)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables core promoter sequence-specific DNA binding

inferred from biological aspect of ancestor with PANTHER:PTN000115754

(assigned by GO_Central )

(Gaudet et al., 2011)

enables general transcription initiation factor activity

inferred from electronic annotation with InterPro:IPR042225

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables protein heterodimerization activity

inferred from electronic annotation with InterPro:IPR009072

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables TBP-class protein binding

inferred from biological aspect of ancestor with PANTHER:PTN000115754

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (8 terms)

Terms Based on Experimental Evidence (7 terms)

CV Term

Evidence

References

involved_in chromatin remodeling

inferred from direct assay

(Suganuma et al., 2008)

involved_in histone acetylation

inferred from direct assay

(Suganuma et al., 2008)

involved_in negative regulation of transcription by RNA polymerase II

inferred from mutant phenotype

(Hsu et al., 2008)

inferred from direct assay

(Willy et al., 2000)

involved_in negative regulation of transcription, DNA-templated

inferred from direct assay

(assigned by UniProt )

(Willy et al., 2000)

involved_in nucleosome mobilization

inferred from direct assay

(Suganuma et al., 2008)

involved_in positive regulation of transcription by RNA polymerase II

inferred from mutant phenotype

(Hsu et al., 2008)

inferred from direct assay

(Willy et al., 2000)

involved_in positive regulation of transcription, DNA-templated

inferred from direct assay

(assigned by UniProt )

(Willy et al., 2000)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in chromatin remodeling

inferred from biological aspect of ancestor with PANTHER:PTN000115754

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in negative regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN000115754

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in positive regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN000115754

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in RNA polymerase II preinitiation complex assembly

inferred from biological aspect of ancestor with PANTHER:PTN000115754

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

part_of Ada2/Gcn5/Ada3 transcription activator complex

inferred from physical interaction with FLYBASE:Ada2a; FB:FBgn0263738

(Suganuma et al., 2008)

inferred from direct assay

(Suganuma et al., 2012)

located_in nucleus

inferred from direct assay

(assigned by UniProt )

(Willy et al., 2000)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

part_of Ada2/Gcn5/Ada3 transcription activator complex

inferred from biological aspect of ancestor with PANTHER:PTN000115754

(assigned by GO_Central )

(Gaudet et al., 2011)

part_of negative cofactor 2 complex

inferred from biological aspect of ancestor with PANTHER:PTN000115754

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

ATAC COMPLEX

OTHER DNA BINDING DOMAIN TRANSCRIPTION FACTORS

Protein Family (UniProt)

Belongs to the NC2 beta/DR1 family. (Q9VJQ5)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

ATAC COMPLEX -: The Ada2a-containing (ATAC) complex is a metazoan histone acetyltransferase complex. It positively regulates gene transcription and, although it does not have any intrinsic remodeling activity, facilitates nucleosome sliding of ISWI and SWI-SNF families of chromatin-remodeling complexes. (Adapted from FBrf0204126).
OTHER DNA BINDING DOMAIN TRANSCRIPTION FACTORS -: The Other DNA binding domain transcription factors group is a collection of DNA-binding transcription factors that do not fit into any of the other major domain-based transcription factor groups.

Protein Function (UniProtKB)

(UniProt, Q9VJQ5)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\NC2β for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.45

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

NC2β-RA

FBtr0080695

NM_135892

726

183

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

NC2β-PA

FBpp0080256

20.9

183

4.31

NP_609736

AAF53428

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Component of the Ada2a-containing (ATAC) complex composed of at least Ada2a, Atac1, Hcf, Ada3, Gcn5, Mocs2B, Charac-14, Atac3, Atac2, NC2beta and wds (PubMed:18327268). Homodimer (PubMed:18327268). Interacts with NC2-alpha/Drap1 to form the dNC2 complex (PubMed:11062130).

(UniProt, Q9VJQ5)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VJQ5

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\NC2β using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

NC2beta_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism | ubiquitous

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | faint | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of Ada2/Gcn5/Ada3 transcription activator complex

inferred from physical interaction with FLYBASE:Ada2a; FB:FBgn0263738

(Suganuma et al., 2008)

inferred from direct assay

(Suganuma et al., 2012)

located_in nucleus

inferred from direct assay

(assigned by UniProt )

(Willy et al., 2000)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\NC2β in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\NC2β

View exonic expression by tissue for Dmel\NC2β

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0028926

DRscDB

34875/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0028926

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG4185-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4185

Flygut - An atlas of the Drosophila adult midgut

FBgn0028926

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of NC2β

Transgenic constructs containing regulatory region of NC2β

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2L)Exel7061

(Ryder, 2004.4.26)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

NC2β^GD1590

viable, with Scer\GAL4^Mef2.PR

NC2β^GD1590

viable, with Scer\GAL4^pnr-MD237

NC2β^GD1590

viable, with Scer\GAL4^tin.CΔ4

NC2β^GD1590

Other Phenotypes

Allele

abnormal heat stress response | third instar larval stage, with Scer\GAL4^ppk.1.9

NC2β^NIG.4185R

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^ppk.1.9

NC2β^NIG.4185R

Phenotype manifest in

Allele

dendrite | third instar larval stage, with Scer\GAL4^ppk.1.9

NC2β^NIG.4185R

larval dorsal multidendritic neuron ddaC | third instar larval stage, with Scer\GAL4^ppk.1.9

NC2β^NIG.4185R

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\DR1

13 of 15

Yes

Hsap\FGFR3

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Dr1

13 of 15

Yes

Rattus norvegicus (Norway rat) (2)

Rnor\LOC100910207

12 of 13

Yes

Rnor\Dr1

6 of 13

Yes

Xenopus tropicalis (Western clawed frog) (1)

Xtro\dr1

10 of 12

Yes

Danio rerio (Zebrafish) (1)

Drer\dr1

14 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\dro-1

13 of 15

Yes

Arabidopsis thaliana (thale-cress) (2)

Atha\NF-YB12

7 of 9

Yes

Atha\NF-YB13

7 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\NCB2

12 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPBC30D10.02

11 of 12

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190IBH )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA18013

Drosophila persimilis

Dper\GL26021

Drosophila willistoni

Dwil\GK18069

Drosophila virilis

Dvir\GJ11838

Drosophila mojavensis

Dmoj\GI13215

Drosophila grimshawi

Dgri\GH10911

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150DP1 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17012692

Glossina morsitans

Tsetse fly

Gmor\GMOY000581

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_05387

Mayetiola destructor

Hessian fly

Mdes\Mdes010272

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL008763

Anopheles darlingi

American malaria mosquito

Adar\ADAC003780

Anopheles gambiae

Malaria mosquito

Agam\AGAP010322

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ009231

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0HHV )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA006192

Danaus plexippus

Monarch butterfly

Dple\DPOGS214145-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL015636

Apis florea

Little honeybee

Aflo\2215879188

Apis mellifera

Western honey bee

Amel\GB40387

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP24053

Bombus terrestris

Buff-tailed bumblebee

Bter\15208312

Linepithema humile

Argentine ant

Lhum\LH14810

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:000950

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG012877

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_08601

Tribolium castaneum

Red flour beetle

Tcas\TC014716

Pediculus humanus

Human body louse

Phum\PHUM323600

Rhodnius prolixus

Kissing bug

Rpro\RPRC002484

Cimex lectularius

Bed bug

Clec\CLEC007735

Cimex lectularius

Bed bug

Clec\CLEC003216

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI002304

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_13444

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0LUS )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR005427

Ixodes scapularis

Black-legged tick

Isca\ISCW005635

Stegodyphus mimosarum

African social velvet spider

Smim\KFM60851.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur02g01190

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_49994

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0ZDR )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Dr1

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000020364

Gallus gallus

Domestic chicken

Ggal\DR1

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (2)

Gene Symbol

Score

Source

Alignment

1 of 10

tau

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

DR1; down-regulator of transcription 1

13 of 15

601482

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

NC2β - Ada2a

anti tag coimmunoprecipitation, peptide massfingerprinting, molecular sieving

(Suganuma et al., 2012, Suganuma et al., 2008)

NC2β - Atac1

experimental knowledge based

(Guruharsha et al., 2011)

NC2β - Atac2

experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting, molecular sieving

(Suganuma et al., 2012, Guruharsha et al., 2011, Suganuma et al., 2010, Suganuma et al., 2008)

NC2β - Chrac-14

experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting

(Guruharsha et al., 2011, Suganuma et al., 2010, Suganuma et al., 2008)

NC2β - D12

experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting

(Guruharsha et al., 2011, Suganuma et al., 2010)

NC2β - Dbx

two hybrid array

(Shokri et al., 2019)

NC2β - Gcn5

experimental knowledge based

(Guruharsha et al., 2011)

NC2β - Hcf

experimental knowledge based

(Guruharsha et al., 2011)

NC2β - Jra

anti bait coimmunoprecipitation, western blot

(Suganuma et al., 2010)

NC2β - MBD-R2

experimental knowledge based

(Guruharsha et al., 2011)

NC2β - Mocs2B

anti tag coimmunoprecipitation, western blot, experimental knowledge based, peptide massfingerprinting, anti bait coimmunoprecipitation

(Suganuma et al., 2012, Guruharsha et al., 2011, Suganuma et al., 2010, Suganuma et al., 2008)

NC2β - NC2α

array technology, two hybrid array, bimolecular fluorescence complementation, fluorescence microscopy

(Shokri et al., 2019)

NC2β - Rel

two hybrid array

(Shokri et al., 2019)

NC2β - Sgf29

experimental knowledge based

(Guruharsha et al., 2011)

NC2β - Taf11

two hybrid

(Hu et al., 2017.6.13)

NC2β - Ubx

bimolecular fluorescence complementation, fluorescence microscopy

(Bischof et al., 2018)

NC2β - da

two hybrid array

(Shokri et al., 2019)

NC2β - h

two hybrid array

(Shokri et al., 2019)

NC2β - msn

anti bait coimmunoprecipitation, western blot

(Suganuma et al., 2010)

NC2β - wds

anti tag coimmunoprecipitation, peptide massfingerprinting

(Suganuma et al., 2010, Suganuma et al., 2008)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

(UniProt, Q9VJQ5 )

Linkouts

BioGRID - A database of protein and genetic interactions.

60894

DroID - A comprehensive database of gene and protein interactions.

FBgn0028926

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

34875

MIST (protein-protein) - An integrated Molecular Interaction Database

NC2beta

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-50

Cytogenetic map

35B8-35B8

Sequence location

2L:15,007,813..15,008,538 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

35B8-35B8

Limits computationally determined from genome sequence between P{EP}elB^EP2039&P{PZ}osp^rJ571 and P{lacW}Su(H)^k07904

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-50

(FlyBase, 2016)

2-47.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (8)

Bloomington

56157

w¹¹¹⁸; PBac{NC2β-GFP.FPTB}VK00033

78740

y¹ sc^* v¹ sev²¹; P{TOE.GS02258}attP40

57421

y¹ sc^* v¹ sev²¹; P{TRiP.HMC04728}attP40

FlyORF

F003397

M{UAS-NC2β.ORF-CC}ZH-21F

F004777

M{UAS-NC2β.ORF-VN}ZH-86Fb

F000269

M{UAS-NC2β.ORF.3xHA.GW}ZH-86Fb

VDRC

v3161

w¹¹¹⁸; P{GD1590}v3161

v341476

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD00547}attP40/CyO-GFP

Genomic Clones (19)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (545)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH16072

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0028926

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0028926

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34875

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: BG:DS00929.3 CG4185

(Kodira, 1999.11)

Source for identity of: BG:DS00929.3 NC2β

(FlyBase, 1996-)

Source for database merge of

Additional comments

"l(2)35Bc" probably corresponds to either " BG:DS00929.4 " or to " BG:DS00929.3 ".

(Ashburner et al., 1999)

" BG:DS00929.3 " may correspond to "l(2)35Be" or "l(2)35Bc".

(Ashburner et al., 1999.10.12)

Source for identity of BG:DS00929.3 NC2β was sequence comparison ( date:000225 ).

(FlyBase, 1996-)

Other Comments

Recombinant "NC2" (consisting of both NC2α and NC2β proteins) activates downstream promoter element (DPE)-driven promoters and represses TATA-driven promoters.

(Willy et al., 2000)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 38 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34875

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VJQ5

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0028926

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0028926

DRscDB

34875/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0028926

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

NC2beta_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4185

Flygut - An atlas of the Drosophila adult midgut

FBgn0028926

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34875

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC014716

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG4185

MARRVEL_MODEL

34875

modMine - A data warehouse for the modENCODE project

FBgn0028926

Linkouts

BioGRID - A database of protein and genetic interactions.

60894

DroID - A comprehensive database of gene and protein interactions.

FBgn0028926

DRSC - Results frm RNAi screens

FBgn0028926

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG4185-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0028926

FlyMine - An integrated database for Drosophila genomics

FBgn0028926

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

34875

MIST (protein-protein) - An integrated Molecular Interaction Database

NC2beta

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

BG:DS00929.3

(Bolshakov et al., 2002, Ashburner et al., 1999, Ashburner et al., 1999.10.12)

CG4185

(Honjo et al., 2016, Ni et al., 2010.12.1, Aoyagi and Wassarman, 2000)

Dr1

(Stathopoulos et al., 2002, Phippen et al., 2001, Aoyagi and Wassarman, 2000, Willy, 2000.8.31)

NC2 beta

(Honjo et al., 2016, Suganuma et al., 2012, Suganuma et al., 2010)

NC2beta

(Bischof et al., 2018, Transgenic RNAi Project members, 2017-, Japanese National Institute of Genetics, 2012.5.21, Huen and Roote, 2010.5.4, Giot et al., 2003)

NC2β

(Yamaguchi et al., 2021, Shokri et al., 2019, Hu et al., 2017.6.13, Schertel et al., 2015, Victorsen and White, 2014.5.13, Spedale et al., 2012, Suganuma et al., 2012, Nagy et al., 2010, Hsu et al., 2008, Suganuma et al., 2008)

Nc2b

(Furrer et al., 2010)

dDr1

(Nibu, 2005)

dNC2

(Rosenberg et al., 2001)

dNC2β

(Spedale et al., 2012)

Name Synonyms

NC2β

Negative Cofactor 2β

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (61)

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