FlyBase Allele Report: Dmel\Csk[j1D8]

Csk^c04256/Csk^j1D8 third instar larvae do not show significant electrophysiological defects (mEPSPs, EPSPs and quantal content similar to wild type).

(Spring et al., 2016)

Wing discs of homozygous third instar larvae show considerable apoptosis.

(Langton et al., 2007)

Mutant larvae die within 6-8 hours after pupariation. The larval body mass of mutants increases up to 180% of the wild-type mass during the wandering stage. The brain ventral ganglion and salivary glands of mutants are enlarged. Pupariation is delayed. The body length of mutant pupae are about 121% the size of wild-type. Imaginal discs of mutant larvae are also larger than wild-type. The larval body mass of Csk^j1D8/Csk^S030003 mutants increases up to 150% of the wild-type mass during the wandering stage. In the rare cases mutants survived to pharate adult stage, the head and eyes are enlarged. The posterior ommatidia are sometimes misaligned, though individual ommatidia are morphologically normal. Mutant eyes contain more ommatidia than wild-type. Rarely the eyes are replaced with duplicated antennae. In addition the wings and legs are severely malformed, the notum is sometimes split, and the head, legs and notum often contain cuticle overgrowths. In Csk^j1D8/Csk^S030003 and homozygous Csk^j1D8 mutant developing eye discs, an increase is seen in the number of mitotic cells in the morphogenetic furrow. Mutant pupal retinas contain excess mitotic cells in the interommatidial lattice. Csk^j1D8/Csk^S030003 eye antennal disc cells exhibit a decrease in the proportion of cells in G₀-G₁ phase, and a consequent increase in cells in G₂-M phase when compared to wild-type controls. Homozygous mutant clones in the eye produced severely overgrown eyes. Eyes have more than 1200 ommatidia, compared to about 687 in wild-type. Eyes can become malformed as a result. The extra ommatidia are occasionally inverted in their planar polarity but are otherwise normal in size and morphology. Occasionally mutant clones result in antennal duplication and cuticle overgrowth.

(Read et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Csk^j1D8, puc^E69/puc[+] has visible phenotype, enhanceable by ASPP[+]/ASPP⁸

(Langton et al., 2007)

Csk^j1D8 has abnormal size | adult stage | somatic clone phenotype, enhanceable by Stat92E^j6C8

(Read et al., 2004)

Suppressed by

Statement

Reference

ASPP^d/ASPP⁸, Csk^j1D8 has visible | adult stage phenotype, suppressible by ASPP^Ubi-p63E.GFP

(Bertran et al., 2019)

ASPP^d/ASPP⁸, Csk^j1D8 has visible | adult stage phenotype, suppressible | partially by ASPP^{FA.Ubi-p63E.GFP}

(Bertran et al., 2019)

ASPP⁸, Csk^j1D8/Csk[+] has visible phenotype, suppressible by Btk29A[+]/Btk^k00206

(Langton et al., 2007)

Csk^j1D8/Csk^S030003 has lethal | pupal stage phenotype, suppressible by Btk^k00206

(Read et al., 2004)

Csk^j1D8/Csk^S030003 has lethal | pupal stage phenotype, suppressible by Src64B^PI

(Read et al., 2004)

Csk^j1D8/Csk^S030003 has lethal | pupal stage phenotype, suppressible by bsk¹

(Read et al., 2004)

Csk^j1D8/Csk^S030003 has lethal | pupal stage phenotype, suppressible | partially by Src42A^18-2

(Read et al., 2004)

Csk^j1D8/Csk^S030003 has abnormal mitotic cell cycle phenotype, suppressible by Btk^k00206

(Read et al., 2004)

Csk^j1D8/Csk^S030003 has increased occurrence of cell division phenotype, suppressible by bsk¹

(Read et al., 2004)

Csk^j1D8/Csk^S030003 has increased occurrence of cell division phenotype, suppressible by Btk^k00206

(Read et al., 2004)

NOT suppressed by

Statement

Reference

Csk^j1D8/Csk^S030003 has lethal | pupal stage phenotype, non-suppressible by CadN^M12

(Read et al., 2004)

Csk^j1D8/Csk^S030003 has lethal | pupal stage phenotype, non-suppressible by Egfr^f2

(Read et al., 2004)

Csk^j1D8/Csk^S030003 has lethal | pupal stage phenotype, non-suppressible by Jra^IA109

(Read et al., 2004)

Csk^j1D8/Csk^S030003 has lethal | pupal stage phenotype, non-suppressible by Sos^34Ea-6

(Read et al., 2004)

Csk^j1D8/Csk^S030003 has lethal | pupal stage phenotype, non-suppressible by Src42A^Su(Raf)1-1

(Read et al., 2004)

Csk^j1D8/Csk^S030003 has lethal | pupal stage phenotype, non-suppressible by Src42A^k10108

(Read et al., 2004)

Csk^j1D8/Csk^S030003 has lethal | pupal stage phenotype, non-suppressible by chico¹

(Read et al., 2004)

Csk^j1D8/Csk^S030003 has lethal | pupal stage phenotype, non-suppressible by ft^G-rv

(Read et al., 2004)

Csk^j1D8/Csk^S030003 has lethal | pupal stage phenotype, non-suppressible by scb²

(Read et al., 2004)

Csk^j1D8/Csk^S030003 has lethal | pupal stage phenotype, non-suppressible by shg^k03401

(Read et al., 2004)

Enhancer of

Statement

Reference

Csk^j1D8/Csk[+] is an enhancer of abnormal neuroanatomy phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Saja et al., 2010)

Csk^j1D8/Csk[+] is an enhancer of visible phenotype of Ret^MEN2B.GMR

(Read et al., 2005)

Csk^j1D8/Csk[+] is an enhancer of visible phenotype of Ret^GMR.PR

(Read et al., 2005)

Csk^j1D8 is an enhancer of abnormal size | adult stage | somatic clone phenotype of Stat92E^j6C8

(Read et al., 2004)

Csk^j1D8 is an enhancer of increased cell number phenotype of upd1^GMR.PB

(Read et al., 2004)

NOT Enhancer of

Statement

Reference

Csk^j1D8/Csk[+] is a non-enhancer of visible | adult stage phenotype of Magi^UAS.GFP, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

Csk^j1D8/Csk[+] is a non-enhancer of visible | dominant phenotype of Egfr^E1

(Read et al., 2005)

Csk^j1D8/Csk[+] is a non-enhancer of visible phenotype of Ret^MEN2A.GMR

(Read et al., 2005)

Suppressor of

Statement

Reference

Csk^j1D8/Csk[+] is a suppressor of visible phenotype of Scer\GAL4^sev.PU, Zzzz\CTG^i480.UAS.cGa

(Garcia-Lopez et al., 2008)

NOT Suppressor of

Statement

Reference

Csk^j1D8/Csk[+] is a non-suppressor of visible | adult stage phenotype of Magi^UAS.GFP, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

Csk^j1D8/Csk[+] is a non-suppressor of visible phenotype of Ret^MEN2A.GMR

(Read et al., 2005)

Csk^j1D8/Csk[+] is a non-suppressor of visible | dominant phenotype of Egfr^E1

(Read et al., 2005)

Other

Statement

Reference

ASPP^d/ASPP⁸, Csk^j1D8 has visible | adult stage phenotype

(Bertran et al., 2019)

Csk^j1D8/Csk^c04256, Fas2^e76, GluRIIA^SP16 has abnormal neuroanatomy | third instar larval stage phenotype

(Spring et al., 2016)

Csk^j1D8/Csk^c04256, Fas2^e76, GluRIIA^SP16 has abnormal neurophysiology | third instar larval stage phenotype

(Spring et al., 2016)

Csk^j1D8/Csk^c04256, GluRIIA^SP16 has abnormal neurophysiology | third instar larval stage phenotype

(Spring et al., 2016)

Csk^j1D8/Csk[+], GluRIIA^SP16, cac^S/cac[+] has abnormal neurophysiology | third instar larval stage phenotype

(Spring et al., 2016)

Csk^j1D8/Csk^c04256, GluRIIA^SP16, Src64B[+]/Src64B^ko has abnormal neurophysiology | third instar larval stage phenotype

(Spring et al., 2016)

Csk^UAS.YFP, Csk^j1D8/Csk^c04256, GluRIIA^SP16, Scer\GAL4^nau.PM has abnormal neurophysiology | third instar larval stage phenotype

(Spring et al., 2016)

ASPP^d, Csk^j1D8 has visible phenotype

(Langton et al., 2009)

ASPP⁸, Csk^j1D8/Csk[+] has visible phenotype

(Langton et al., 2007)

ASPP⁸, Csk^j1D8/Csk[+] has increased cell death | third instar larval stage phenotype

(Langton et al., 2007)

Csk^j1D8, puc^E69/puc[+] has increased cell death phenotype

(Langton et al., 2007)

Csk^j1D8, puc^E69/puc[+] has visible phenotype

(Langton et al., 2007)

ASPP[+]/ASPP⁸, Csk^j1D8, puc^E69/puc[+] has visible phenotype

(Langton et al., 2007)

Csk^j1D8, Stat92E⁰⁶³⁴⁶ has increased cell death phenotype

(Read et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

Csk^j1D8, puc^E69/puc[+] has wing phenotype, enhanceable by ASPP[+]/ASPP⁸

(Langton et al., 2007)

Csk^j1D8 has eye-antennal disc | somatic clone phenotype, enhanceable by Stat92E⁰⁶³⁴⁶

(Read et al., 2004)

Csk^j1D8 has eye | somatic clone phenotype, enhanceable by Stat92E^j6C8

(Read et al., 2004)

Suppressed by

Statement

Reference

ASPP^d/ASPP⁸, Csk^j1D8 has wing phenotype, suppressible by ASPP^Ubi-p63E.GFP

(Bertran et al., 2019)

ASPP^d/ASPP⁸, Csk^j1D8 has wing phenotype, suppressible | partially by ASPP^{FA.Ubi-p63E.GFP}

(Bertran et al., 2019)

ASPP⁸, Csk^j1D8/Csk[+] has wing phenotype, suppressible by Btk29A[+]/Btk^k00206

(Langton et al., 2007)

ASPP⁸, Csk^j1D8/Csk[+] has wing disc phenotype, suppressible by Btk29A[+]/Btk^k00206

(Langton et al., 2007)

Csk^j1D8, Stat92E^j6C8 has eye | somatic clone phenotype, suppressible by Btk^k00206

(Read et al., 2004)

Enhancer of

Statement

Reference

Csk^j1D8/Csk[+] is an enhancer of eye phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Saja et al., 2010)

Csk^j1D8/Csk[+] is an enhancer of eye phenotype of Ret^MEN2B.GMR

(Read et al., 2005)

Csk^j1D8/Csk[+] is an enhancer of eye phenotype of Ret^GMR.PR

(Read et al., 2005)

Csk^j1D8, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, Src42A^UAS.cPa

(Pedraza et al., 2004)

Csk^j1D8, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, Src64B^UAS.cPa

(Pedraza et al., 2004)

Csk^j1D8 is an enhancer of eye phenotype of upd1^GMR.PB

(Read et al., 2004)

Csk^j1D8 is an enhancer of eye | somatic clone phenotype of Stat92E^j6C8

(Read et al., 2004)

NOT Enhancer of

Statement

Reference

Csk^j1D8/Csk[+] is a non-enhancer of eye | adult stage phenotype of Magi^UAS.GFP, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

Csk^j1D8/Csk[+] is a non-enhancer of eye phenotype of Egfr^E1

(Read et al., 2005)

Csk^j1D8/Csk[+] is a non-enhancer of eye phenotype of Ret^MEN2A.GMR

(Read et al., 2005)

Suppressor of

Statement

Reference

Csk^j1D8/Csk[+] is a suppressor of eye phenotype of Scer\GAL4^sev.PU, Zzzz\CTG^i480.UAS.cGa

(Garcia-Lopez et al., 2008)

Csk^j1D8 is a suppressor of ommatidium phenotype of wts^GMR.PS

(Stewart et al., 2003)

Csk^j1D8 is a suppressor of eye phenotype of wts^GMR.PS

(Stewart et al., 2003)

NOT Suppressor of

Statement

Reference

Csk^j1D8/Csk[+] is a non-suppressor of eye | adult stage phenotype of Magi^UAS.GFP, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

Csk^j1D8/Csk[+] is a non-suppressor of eye phenotype of Ret^MEN2A.GMR

(Read et al., 2005)

Csk^j1D8/Csk[+] is a non-suppressor of eye phenotype of Egfr^E1

(Read et al., 2005)

Other

Statement

Reference

ASPP^d/ASPP⁸, Csk^j1D8 has wing phenotype

(Bertran et al., 2019)

Csk^j1D8/Csk^c04256, Fas2^e76, GluRIIA^SP16 has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Spring et al., 2016)

Csk^j1D8/Csk^c04256, GluRIIA^SP16 has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Spring et al., 2016)

Csk^j1D8/Csk[+], GluRIIA^SP16, cac^S/cac[+] has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Spring et al., 2016)

Csk^j1D8/Csk^c04256, GluRIIA^SP16, Src64B[+]/Src64B^ko has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Spring et al., 2016)

Csk^UAS.YFP, Csk^j1D8/Csk^c04256, GluRIIA^SP16, Scer\GAL4^nau.PM has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Spring et al., 2016)

ASPP^d, Csk^j1D8 has wing phenotype

(Langton et al., 2009)

Csk^j1D8, puc^E69/puc[+] has wing phenotype

(Langton et al., 2007)

ASPP⁸, Csk^j1D8/Csk[+] has wing phenotype

(Langton et al., 2007)

ASPP⁸, Csk^j1D8/Csk[+] has wing disc phenotype

(Langton et al., 2007)

Csk^j1D8, puc^E69/puc[+] has wing pouch phenotype

(Langton et al., 2007)

ASPP[+]/ASPP⁸, Csk^j1D8, puc^E69/puc[+] has leg phenotype

(Langton et al., 2007)

Csk^j1D8, Stat92E⁰⁶³⁴⁶ has antenna | somatic clone phenotype

(Read et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

ASPP^d/ASPP⁸, Csk^j1D8/+ adults very frequently show wing notches, which are never observed in controls; this phenotype is fully rescued by ASPP^Ubi-p63E.GFP, but only partially by ASPP^{FA.Ubi-p63E.GFP}.

(Bertran et al., 2019)

Third instar larval GluRIIA^SP16/GluRIIA^SP16;Csk^c04256/Csk^j1D8 double mutants show significant decreases in mEPSP (decreasing quantal size) but do not show significant increases in quantal content (as seen in GluRIIA^SP16/GluRIIA^SP16 mutants alone) at the NMJ. Additional presence Csk^j1D8/+ does not alter differences in quantal size and content seen in GluRIIA^SP16/GluRIIA^SP16 larvae. GluRIIA^SP16/GluRIIA^SP16 cac^S/+ Csk^j1D8/+ third instar larvae show significant decreases in mEPSP and quantal content at the NMJ.

GluRIIA^SP16/GluRIIA^SP16 Csk^c04256/Csk^j1D8 Src64B^ko/+ third instar larvae show significant decreases in mEPSP (decreased quantal size) partially restores significant compensatory homeostatic increases in quantal content (unlike GluRIIA^SP16/GluRIIA^SP16 Csk^c04256/Csk^j1D8 double mutants, which do not show significant increases in quantal content) at the NMJ.

GluRIIA^SP16/GluRIIA^SP16;Csk^c04256/Csk^j1D8 third instar larvae show significant decreases in mEPSP (decreasing quantal size) but do not show significant compensatory homeostatic increases in quantal content (as seen in GluRIIA^SP16/GluRIIA^SP16 mutants alone) at the NMJ; additional expression of Csk^{Scer\UAS.T:Avic\GFP-YFP} driven by Scer\GAL4^nau.PM or Scer\GAL4^VGlut-OK371 restores phenotypes to those seen in GluRIIA^SP16/GluRIIA^SP16 mutants alone.

GluRIIA^SP16/GluRIIA^SP16;Csk^c04256/Csk^j1D8 third instar larvae show significant decreases in mEPSP (decreasing quantal size) but do not show significant compensatory homeostatic increases in quantal content (as seen in GluRIIA^SP16/GluRIIA^SP16 mutants alone) at the NMJ; additional presence of Fas2^e76 restores phenotypes to those seen in GluRIIA^SP16/GluRIIA^SP16 mutants alone. GluRIIA^SP16/GluRIIA^SP16;Csk^c04256/Csk^j1D8 Fas2^e76 third instar larvae also show a small significant increase in bouton number per muscle area (segment A2, synapse 6/7).

(Spring et al., 2016)

The rough eye phenotype observed in adults expressing Magi^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^GMR.PU is not modified by combination with single copy of Csk^j1D8.

(Zaessinger et al., 2015)

The tumor overgrowth of somatic clones (induced specifically in the eye disc) expressing Ras85D^V12.Scer\UAS under the control of Scer\GAL4^Act.PU is suppressed when the clones are also homozygous for Csk^j1D8.

(Hirabayashi et al., 2013)

A Csk^j1D8/+ background enhances the visual system degeneration seen in Scer\GAL4^GMR.PF, Ppt1^Scer\UAS.cKa flies.

(Saja et al., 2010)

68% of ASPP^d ; Csk^j1D8/+ flies have notched wings.

(Langton et al., 2009)

ASPP⁸/ASPP⁸ Csk^j1D8/+ adults show a notched wing phenotype. Wing discs of ASPP⁸/ASPP⁸ Csk^j1D8/+ third instar larvae show considerable apoptosis. Neither phenotype is seen in ASPP⁸/ASPP⁸ or Csk^j1D8/+ single mutants.

Btk29A^k00206/+ largely suppresses the ectopic apoptosis in wing discs and notched wing phenotype seen in ASPP⁸/ASPP⁸ Csk^j1D8/+ animals.

puc^E69/+ Csk^j1D8/+ double heterozygotes have a notched wing phenotype, and third larval instar wing discs show ectopic apoptosis within the wing pouch.

The ectopic apoptosis seen in the wing discs of puc^E69/+ Csk^j1D8/+ double heterozygotes is enhanced if they are also heterozygous for ASPP⁸, resulting in adults with a severely reduced wing size. These adults also have leg deformities.

(Langton et al., 2007)

Csk^j1D8, Stat92E^j6C8 mutant eyes are smaller than eyes mutant for Csk^j1D8 or Stat92E^j6C8 alone. In addition, adult eyes are frequently fragmented, with scars and/or patches of eye tissue separated by patches of cuticle. Stat92E⁰⁶³⁴⁶. Csk^j1D8 mutant larval eye-antennal discs frequently show reduced sizes relative to the single mutants. Mutants also frequently show a reduction or absence of developing antennal tissues. Mutant larval eye tissue also contains many apoptotic cells.

(Read et al., 2004)

Xenogenetic Interactions

Statement

Reference

Csk^j1D8 dominantly suppresses the rough eye phenotype caused by expression of Zzzz\CTG^{i480.Scer\UAS.cGa} under the control of Scer\GAL4^sev.PU.

(Garcia-Lopez et al., 2008)

Complementation and Rescue Data

Fails to complement

Csk^S017909

(Read et al., 2004)

Csk^S030003

(Read et al., 2004)

Rescued by

Csk^j1D8 is rescued by Csk^hs.PR

(Read et al., 2004)

Csk^j1D8 is rescued by Csk^hs.PS

(Stewart et al., 2003)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

10244

y¹ w¹¹¹⁸; P{3'lacW}j1D8 FB{}j1D8 Csk^j1D8/TM3, Sb¹

Kyoto

111035

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}82B P{3'lacW}j1D8/TM6B, P{Car20y}TPN1, Tb¹

Notes on Origin

Discoverer

Comments

Complements: l(3)04629⁰⁴⁶²⁹.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (12)

Reported As

Symbol Synonym

Csk^1jd8

(Bertran et al., 2019)

Csk^J1D8

(Zaessinger et al., 2015)

Csk^j1D8

(Spring et al., 2016, Saja et al., 2010, Christensen et al., 2008.4.15, O'Reilly et al., 2006)

csk^hypo

(Hirabayashi et al., 2013)

csk^j1D8

(Hirabayashi et al., 2013)

dCsk^1jd8

(Langton et al., 2007)

dCsk^j1D8

(Read et al., 2005, Read et al., 2004)

dCsk^j1d8

(Langton et al., 2009)

dcsk1D8

(Pedraza et al., 2004)

dcsk^flik

(Stewart et al., 2003)

l(3)j1D8

(Garcia-Lopez et al., 2008, Kusano et al., 2001, Spradling et al., 1999)

l(3)j1D8^j1D8

(BDGP Project Members, 1994-1999)

Name Synonyms

l(3)j1D8j1D8

(Stewart et al., 2003)

Secondary FlyBase IDs

FBal0086976

References (18)

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