FlyBase Gene Report: Dmel\eIF4G1

General Information

Symbol

Dmel\eIF4G1

Species

D. melanogaster

Name

eukaryotic translation initiation factor 4G1

Annotation Symbol

CG10811

Feature Type

protein_coding_gene

FlyBase ID

FBgn0023213

Gene Model Status

Current

Stock Availability

11 publicly available

Gene Summary

eukaryotic translation initiation factor 4G1 (eIF4G1) encodes a protein that contributes to translation and spermatogenesis. [Date last reviewed: 2019-07-11] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

eIF4G, eIF-4G, eukaryotic translation initiation factor 4G, deIF4G, Eukaryotic-initiation-factor-4G

Key Links

Genomic Location

Cytogenetic map

102F1-102F1

Sequence location

4:915,297..930,715 [-]

Recombination map

4-0

RefSeq locus

NC_004353 REGION:915297..930715

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

NCBI

UCSC

Ensembl

Function

Gene Ontology (GO) Annotations (10 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables eukaryotic initiation factor 4E binding

inferred from physical interaction with FLYBASE:eIF4E1; FB:FBgn0015218

(Hernandez et al., 2005, Hernandez et al., 1998)

inferred from physical interaction with FLYBASE:eIF4E4; FB:FBgn0035709

(Hernandez et al., 2005)

inferred from physical interaction with FLYBASE:eIF4E5; FB:FBgn0035823

(Hernandez et al., 2005)

inferred from physical interaction with FLYBASE:eIF4E7; FB:FBgn0040368

(Hernandez et al., 2005)

inferred from physical interaction with FLYBASE:eIF4E3; FB:FBgn0265089

(Hernandez et al., 2005)

enables RNA 7-methylguanosine cap binding

inferred from direct assay

(Franklin-Dumont et al., 2007)

enables RNA binding

inferred from direct assay

(Zapata et al., 1994)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables RNA binding

inferred from electronic annotation with InterPro:IPR003890

(InterPro Project Members, 2004-)

enables translation initiation factor activity

inferred from biological aspect of ancestor with PANTHER:PTN000583430

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity

(Lasko, 2000)

inferred from sequence or structural similarity with UniProtKB:Q80XI3

(Gene Ontology Curators, 2002-)

Biological Process (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in mitotic cell cycle

inferred from high throughput mutant phenotype

(Ducat et al., 2008)

involved_in regulation of translation

inferred from mutant phenotype

(Franklin-Dumont et al., 2007)

involved_in translation

inferred from direct assay

(Zapata et al., 1994)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in translational initiation

inferred from biological aspect of ancestor with PANTHER:PTN000583430

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity

(Lasko, 2000)

inferred from sequence or structural similarity with UniProtKB:P39935

(Hernandez et al., 1998)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in cytosol

inferred from high throughput direct assay

(Lye et al., 2014)

part_of eukaryotic translation initiation factor 4F complex

inferred from direct assay

(Igreja et al., 2014, Zapata et al., 1994)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in cytosol

inferred from sequence or structural similarity

(Lasko, 2000)

part_of eukaryotic translation initiation factor 4F complex

inferred from biological aspect of ancestor with PANTHER:PTN000583430

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity

(Lasko, 2000)

Gene Group (FlyBase)

CYTOPLASMIC TRANSLATION INITIATION FACTORS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

eukaryotic translation initiation factor 4G1 (eIF4G1) encodes a protein that contributes to translation and spermatogenesis. [Date last reviewed: 2019-07-11]

Gene Group (FlyBase)

CYTOPLASMIC TRANSLATION INITIATION FACTORS -: Cytoplasmic (or eukaryotic) translation initiation factors function in the initiation stage of ribosome-mediated translation in the cytoplasm. (Adapted from FBrf0233552 and PMID:20094052).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\eIF4G1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A8DZ29)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

eIF4G1-RA

FBtr0089243

NM_079901

5576

1666

eIF4G1-RB

FBtr0112904

NM_001103382

6335

1919

eIF4G1-RC

FBtr0289951

NM_001144385

5806

1666

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

eIF4G1-PA

FBpp0088303

183.9

1666

7.92

FBpp0111817

211.1

1919

8.54

FBpp0288389

183.9

1666

7.92

O61380

NP_001137857

ACL82868

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1666 aa isoforms: eIF4G1-PA, eIF4G1-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\eIF4G1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

eIF4G_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.86

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | faint | ubiquitous

(Fisher et al., 2012)

spermatogenesis & adult stage | male

testis | apical | restricted

(Franklin-Dumont et al., 2007)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male & spermatogenesis

cyst cell of testis

(Ghosh and Lasko, 2015)

spermatogonium

• cytoplasm

(Ghosh and Lasko, 2015)

primary spermatocyte

• cytoplasm

(Ghosh and Lasko, 2015)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female

ovary

(Pisa et al., 2009)

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood | male -- day 7 of adulthood | male

spermatozoon

(Wasbrough et al., 2010)

Additional Descriptive Data

eIF4G1 protein is expressed primarily in somatic cyst cells. eIF4G1 protein can also be detected at lower levels in germ cells at some stages of spermatogeneis; it is localized to the cytoplasm of spermatogonia and primary spermatocytes, but is excluded from meiotic and post-meiotic stage germ cells.

(Ghosh and Lasko, 2015)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytosol

inferred from high throughput direct assay

(Lye et al., 2014)

part_of eukaryotic translation initiation factor 4F complex

inferred from direct assay

(Igreja et al., 2014, Zapata et al., 1994)

Expression Deduced from Reporters

Reporter: PBac{754.P.FSVS-0}eIF4G1^CPTI002576

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain cell body rind

(Knowles-Barley, 2011.8.24)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\eIF4G1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\eIF4G1

View exonic expression by tissue for Dmel\eIF4G1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0023213

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

43839/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0023213

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0023213

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0023213

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10811

Flygut - An atlas of the Drosophila adult midgut

FBgn0023213

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 7 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of eIF4G1

Transgenic constructs containing regulatory region of eIF4G1

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(4)C3

(Podemski et al., 2004)

Df(4)O2

(Marygold et al., 2007)

Inferred from location ( 9 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

lethal, with Scer\GAL4^pnr-MD237

eIF4G1^GD7098

lethal, with Scer\GAL4^Ubi.PU

eIF4G1^HMS00762

lethal (with Df(4)8-M91)

lethal (with Df(4)8-M318)

lethal (with Df(4)O2)

lethal (with eIF4G1^B)

eIF4G1^{MI05019-TG4.0}

lethal (with eIF4G1^E)

eIF4G1^{MI05019-TG4.0}

lethal (with eIF4G1^{MI05019-TG4.0})

lethal - all die before end of second instar larval stage, with Scer\GAL4^Mef2.PR

eIF4G1^GD7098

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

eIF4G1^GD7098

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

eIF4G1^GD7098

viable, with Scer\GAL4^tin.CΔ4

eIF4G1^GD7098

Sterility

Allele

female sterile, with Scer\GAL4^{VP16.nanos.UTR}

eIF4G1^HMS00762

male fertile, with Scer\GAL4^VP16.bam

eIF4G1^HMS00762

male fertile, with Scer\GAL4^{VP16.nanos.UTR}

eIF4G1^HMS00762

male semi-sterile, with Scer\GAL4^C587

eIF4G1^HMS00762

Other Phenotypes

Allele

abnormal neuroanatomy | adult stage, with Scer\GAL4^ple.PF

eIF4G1^HMS00762

decreased cell number | adult stage, with Scer\GAL4^ple.PF

eIF4G1^HMS00762

decreased cell number | spermatogenesis, with Scer\GAL4^tj.PU

eIF4G1^GD7098

Phenotype manifest in

Allele

cyst progenitor cell | decreased number, with Scer\GAL4^tj.PU

eIF4G1^GD7098

dopaminergic PPL1 neuron | adult stage, with Scer\GAL4^ple.PF

eIF4G1^HMS00762

elongation stage spermatid, with Scer\GAL4^C587

eIF4G1^HMS00762

larval somatic muscle cell, with Scer\GAL4^Mef2.PR

eIF4G1^GD7098

primary spermatocyte cyst, with Scer\GAL4^C587

eIF4G1^HMS00762

sarcomere, with Scer\GAL4^Mef2.PR

eIF4G1^GD7098

testis, with Scer\GAL4^C587

eIF4G1^HMS00762

Z disc, with Scer\GAL4^Mef2.PR

eIF4G1^GD7098

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (5)

Hsap\EIF4G3

10 of 14

Yes

Hsap\EIF4G1

9 of 14

Yes

Hsap\EIF4G2

4 of 14

Hsap\PAIP1

2 of 14

Hsap\CTIF

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (6)

Rnor\Eif4g1

10 of 14

Yes

Rnor\Eif4g3

10 of 14

Yes

Rnor\Eif4g2

4 of 14

Rnor\Ctif

1 of 14

Rnor\LOC501116

1 of 14

Yes

Rnor\Paip1

1 of 14

Mus musculus (laboratory mouse) (5)

Mmus\Eif4g1

10 of 14

Yes

Mmus\Eif4g3

10 of 14

Yes

Mmus\Eif4g2

4 of 14

Mmus\Paip1

2 of 14

Mmus\Ctif

1 of 14

Xenopus tropicalis (Western clawed frog) (6)

Xtro\eif4g1

8 of 13

Yes

Xtro\eif4g3

7 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (9)

Drer\eif4g1a

9 of 14

Yes

Drer\eif4g3a

9 of 14

Yes

Drer\eif4g3b

9 of 14

Yes

Drer\eif4g2a

4 of 14

Drer\eif4g2b

4 of 14

Drer\wu:fb16f03

4 of 14

Yes

Drer\paip1

2 of 14

Drer\bzw2

1 of 14

Drer\ctif

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\ifg-1

8 of 14

Yes

Anopheles gambiae (African malaria mosquito) (4)

Agam\AgaP_AGAP002502

8 of 12

Yes

Agam\AgaP_AGAP004750

3 of 12

Agam\AgaP_AGAP011627

2 of 12

Agam\AgaP_AGAP011628

1 of 12

Arabidopsis thaliana (thale-cress) (7)

Atha\EIF4G

8 of 13

Yes

Atha\eIFiso4G1

4 of 13

Atha\eIFiso4G2

4 of 13

Atha\AT1G62410

3 of 13

Yes

Atha\AT4G30680

3 of 13

Yes

Atha\AT3G48390

1 of 13

Atha\ECIP1

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\TIF4631

7 of 13

Yes

Scer\TIF4632

6 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\tif471

7 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:eIF4G1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (3)

eIF4G2

7 of 13

NAT1

6 of 13

Paip1

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

congenital heart defect (postulated), EIF4G3-related

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

eIF4G1^HMS00762

ameliorates retinitis pigmentosa 4

modeled by ninaE^P37H.GFP

(Zhao et al., 2023)

exacerbates cancer

modeled by Rbf^HMS03004

(Parkhitko et al., 2021)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

EIF4G3; eukaryotic translation initiation factor 4 gamma 3

10 of 14

603929

EIF4G1; eukaryotic translation initiation factor 4 gamma 1

9 of 14

600495

PARKINSON DISEASE 18, AUTOSOMAL DOMINANT, SUSCEPTIBILITY TO; PARK18

EIF4G2; eukaryotic translation initiation factor 4 gamma 2

4 of 14

602325

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

eIF4G1 - Akt

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eIF4G1 - Capr

anti bait coimmunoprecipitation, western blot

(Papoulas et al., 2010)

eIF4G1 - Fmr1

anti bait coimmunoprecipitation, western blot, peptide massfingerprinting

(Papoulas et al., 2010)

eIF4G1 - Mkrn1

anti bait coimmunoprecipitation, anti tag western blot, anti tag coimmunoprecipitation, Identification by mass spectrometry

(Dold et al., 2020)

eIF4G1 - Pdk1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eIF4G1 - RagC-D

pull down, anti tag western blot, anti tag coimmunoprecipitation, peptide massfingerprinting

(Tsokanos et al., 2016)

eIF4G1 - Tsc1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eIF4G1 - aub

anti tag coimmunoprecipitation, anti tag western blot

(Ma et al., 2017)

eIF4G1 - eIF4A

two hybrid

(Hernandez et al., 2004)

eIF4G1 - eIF4E3

two hybrid, anti bait coimmunoprecipitation, western blot

(Ghosh and Lasko, 2015, Hernandez et al., 2012, Hernandez et al., 2005)

eIF4G1 - eIF4E4

two hybrid, classical fluorescence spectroscopy, predetermined participant

(Zuberek et al., 2016, Hernandez et al., 2005)

eIF4G1 - eIF4E5

classical fluorescence spectroscopy, predetermined participant, two hybrid

(Zuberek et al., 2016, Hernandez et al., 2005)

eIF4G1 - eIF4E7

classical fluorescence spectroscopy, predetermined participant, two hybrid

(Zuberek et al., 2016, Hernandez et al., 2005)

eIF4G1 - foxo

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eIF4G1 - ft

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

eIF4G1 - gig

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eIF4G1 - ksr

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

eIF4G1 - pAbp

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot, Identification by mass spectrometry

(Wang et al., 2017, Ghosh and Lasko, 2015, Zekri et al., 2009)

eIF4G1 - rictor

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eIF4G1 - sima

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eIF4G1 - sstn

anti tag coimmunoprecipitation, peptide massfingerprinting

(Liu et al., 2015)

RNA-protein

Physical Interaction

Assay

References

eIF4G1 - nanos

pull down, western blot

(Jeske et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Pink1

enhanceable

eIF4G1

(Gehrke et al., 2015)

upd1

suppressible

eIF4G1

(Wang et al., 2025)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

68664

DroID - A comprehensive database of gene and protein interactions.

FBgn0023213

MIST (protein-protein) - An integrated Molecular Interaction Database

43839

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

gene_with_edited_transcript

(Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

4-0

Cytogenetic map

102F1-102F1

Sequence location

4:915,297..930,715 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

102F1-102F1

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

102E-102E

(Hernandez, 1997.10.16)

102E-102E

(determined by in situ hybridisation)

(Hernandez et al., 1998)

Experimentally Determined Recombination Data

Location

4-0

(FlyBase, 2016)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (11)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

82960

y¹ v¹; P{TRiP.HMS06053}attP2

76985

y¹ sc^* v¹ sev²¹; P{TKO.GS00738}attP40

78670

y¹ sc^* v¹ sev²¹; P{TOE.GS01928}attP40

33049

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00762}attP2

60779

y¹; Mi{MIC}eIF4G1^MI05019/In(4)ci^D, ci^D pan^ciD

605970

y¹ w¹¹¹⁸; TI{TI}FRT101F eIF4G1^B/In(4)ci^D, ci^D pan^ciD

605971

y¹ w¹¹¹⁸; TI{TI}FRT101F eIF4G1^E/In(4)ci^D, ci^D pan^ciD

76667

y¹ w^*; Mi{Trojan-GAL4.0}eIF4G1^{MI05019-TG4.0}/In(4)ci^D, ci^D pan^ciD

Kyoto

115305

w¹¹¹⁸; PBac{754.P.FSVS-0}eIF4G1^CPTI002576

VDRC

v17002

w¹¹¹⁸; P{GD7098}v17002

v17003

w¹¹¹⁸; P{GD7098}v17003

Genomic Clones (13)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (107)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE51676

Other clones

FI02056

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0023213

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0023213

Antibody Information

Laboratory Generated Antibodies

monoclonal

(Na et al., 2016)

polyclonal

(Ghosh and Lasko, 2015, Peter et al., 2015, Zapata et al., 1994)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Candidate stable intronic sequence RNA (sisRNA) identified within CDS of this gene.

(Pek et al., 2015)

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly short, monopolar spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

eIF-4G has been cloned and sequenced.

(Hernandez et al., 1998)

Drosophila initiation factor eIF-4F is composed of two subunits, one is encoded by eIF-4E and the other by eIF-4G, a 200kD protein. eIF-4G is required for the translation of endogenous mRNAs in cell-free systems made from Drosophila embryos, and can cross-link uncapped, but not m⁷G capped RNA. eIF-4E cross-links with m⁷G capped RNA.

(Zapata et al., 1994)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: eIF4G eIF-4G

(Fuller and Baker, 2007.6.28)

Source for identity of: eIF4G1 eIF4G

(Marygold et al., 2017)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (20)

Reported As

Symbol Synonym

EIF4G

(Kondo and Perrimon, 2011)

EIF4G1

(Damulewicz et al., 2025, Malik et al., 2023, Ham et al., 2021)

Eif4G

deIF4G

(Garrey et al., 2010, Takahashi et al., 2005)

eIF-4G

(Liu and Lasko, 2015, Ducat et al., 2008, Fuller and Baker, 2007.6.28, Goshima et al., 2007, Johansson et al., 2007, Marygold et al., 2007, Stuart et al., 2007, Tadros et al., 2007, Bjorklund et al., 2006, Valencia-Sanchez et al., 2006, Greil et al., 2003)

eIF4G

(Zhao et al., 2023, Frappaolo et al., 2022, Parkhitko et al., 2021, Dold et al., 2020, Lasko, 2020, Vandehoef et al., 2020, Vasudevan et al., 2020, Laflamme et al., 2017, Ma et al., 2017, Rissland et al., 2017, Transgenic RNAi Project members, 2017-, Wang et al., 2017, Grüner et al., 2016, Na et al., 2016, Vinayagam et al., 2016, Zhang et al., 2016, Zuberek et al., 2016, Dent et al., 2015, Gehrke et al., 2015, Ghosh and Lasko, 2015, Horan et al., 2015, Liu et al., 2015, Pek et al., 2015, Peter et al., 2015, Peter et al., 2015, Sopko et al., 2015, Vanhauwaert and Verstreken, 2015, Ashwal-Fluss et al., 2014, Ghosh et al., 2014, Igreja et al., 2014, Cui et al., 2013, Hernández et al., 2013, Kwon et al., 2013, Kwon et al., 2013, Kwon et al., 2013, Yoshigi et al., 2013, Zekri et al., 2013, Gonsalvez and Long, 2012, Hernandez et al., 2012, Jansen and Niessing, 2012, Killip and Grewal, 2012, Mihailovich et al., 2012, Vazquez-Pianzola and Suter, 2012, Friedman et al., 2011, Garrey et al., 2010, Jäger and Dorner, 2010, Müller et al., 2010, Papoulas et al., 2010, Wasbrough et al., 2010, Duncan et al., 2009, Iwasaki et al., 2009, Pisa et al., 2009, Zekri et al., 2009, Duncan, 2008, Hernandez et al., 2008, Baker and Fuller, 2007, Franklin-Dumont et al., 2007, Franklin-Dumont et al., 2007, Fuller and Baker, 2007.6.28, Baker and Fuller, 2006, Hernandez and Vazquez-Pianzola, 2005, Hernandez et al., 2005, Macdonald, 2005, Hernandez et al., 2004, Kozak, 2004, Macdonald, 2004, Nelson et al., 2004, Zappavigna et al., 2004, Gamberi et al., 2003, Herold et al., 2003, Herold et al., 2001, Johnstone and Lasko, 2001, Lasko, 2000, Locke et al., 2000, Lehner, 1999, Hernandez et al., 1998, Zapata et al., 1994)

eIF4G1

(Dyson et al., 2025, Wippich et al., 2023, Ekure et al., 2021, Joseph and Lai, 2021, Liu and Han, 2021.4.14, Rode et al., 2018, Marygold et al., 2017, Gene Disruption Project members, 2016-)

eIF4g

(Ephrussi and St. Johnston, 2004)

eiF-4G

(Okuda et al., 2022)

p200

(Zapata et al., 1994)

Name Synonyms

Eukaryotic initiation factor 4G

(Gouw et al., 2009)

Eukaryotic-initiation-factor-4G

(Chang et al., 2008)

eIF-4G

(Johansson et al., 2007)

eukaryotic

(Hallier et al., 2015)

eukaryotic initiation factor 4G

(Papoulas et al., 2010)

eukaryotic translation initiation factor 4G

(Yoshigi et al., 2013, Garrey et al., 2010, Pisa et al., 2009)

eukaryotic translation initiation factor 4G1

translation initiation factor

(Cho et al., 2005)

translation initiation factor 4G

(Hallier et al., 2015)

translation initiation factor eIF4G

(Hernandez, 1997.10.16)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 59 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

43839

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A8DZ29

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A8DZ29

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0023213

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

43839/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0023213

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0023213

FlyMine - An integrated database for Drosophila genomics

FBgn0023213

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG10811

MARRVEL_MODEL - MARRVEL (model organism gene)

43839

PDB - An information portal to biological macromolecular structures

Linkouts

BioGRID - A database of protein and genetic interactions.

68664

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0023213

DroID - A comprehensive database of gene and protein interactions.

FBgn0023213

DRSC - Results frm RNAi screens

FBgn0023213

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

eIF4G_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0023213

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0023213

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10811

Flygut - An atlas of the Drosophila adult midgut

FBgn0023213

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0023213

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC013876

MIST (protein-protein) - An integrated Molecular Interaction Database

43839

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (165)

The following external sites may use different assemblies or annotations than FlyBase.

-1.86

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