FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\Prosβ5
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General Information
Symbol
Dmel\Prosβ5
Species
D. melanogaster
Name
Proteasome β5 subunit
Annotation Symbol
CG12323
Feature Type
protein_coding_gene
FlyBase ID
FBgn0029134
Gene Model Status
Current
Stock Availability
12 publicly available
Gene Summary
Proteasome β5 subunit (Prosβ5) encodes an endopeptidase subunit of the of the 26S proteasome. [Date last reviewed: 2019-09-26] (FlyBase Gene Snapshot)
All Summaries
Also Known As

β5, dβ5

Key Links
Genomic Location
Cytogenetic map
47C1-47C1
Sequence location
Recombination map
2-63
RefSeq locus
NT_033778 REGION:10819978..10821178
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (10 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables endopeptidase activity
inferred from biological aspect of ancestor with PANTHER:PTN000175708
(GO Reference Genome Project, 2011-)
inferred by curator from GO:0005839
(Sumegi et al., 2003)
inferred from sequence or structural similarity with SGD:S000006307
(Gene Ontology Curators, 2002-)
enables threonine-type endopeptidase activity
inferred from electronic annotation with InterPro:IPR000243
(InterPro Project Members, 2004-)
Biological Process (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
involved_in centrosome cycle
inferred from mutant phenotype
(Goshima et al., 2007)
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process
inferred from mutant phenotype
(Lőw et al., 2013)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process
inferred by curator from GO:0005839
(Sumegi et al., 2003)
inferred from biological aspect of ancestor with PANTHER:PTN008521424
(GO Reference Genome Project, 2011-)
inferred from sequence or structural similarity with SGD:S000006307
(Gene Ontology Curators, 2002-)
Cellular Component (6 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
located_in cytosol
inferred from high throughput direct assay
(Lye et al., 2014)
located_in nucleoplasm
inferred from high throughput direct assay
(Lye et al., 2014)
part_of proteasome complex
inferred from direct assay
(Nickell et al., 2009)
part_of proteasome core complex
inferred from direct assay
(Sumegi et al., 2003)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
is_active_in cytosol
inferred from biological aspect of ancestor with PANTHER:PTN002613347
(GO Reference Genome Project, 2011-)
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN002613348
(GO Reference Genome Project, 2011-)
part_of proteasome core complex, beta-subunit complex
inferred from biological aspect of ancestor with PANTHER:PTN002613347
(GO Reference Genome Project, 2011-)
inferred from sequence or structural similarity with SGD:S000006307
(Gene Ontology Curators, 2002-)
part_of proteasome core complex
inferred from electronic annotation with InterPro:IPR000243, InterPro:IPR001353, InterPro:IPR016050
(InterPro Project Members, 2004-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
Proteasome β5 subunit (Prosβ5) encodes an endopeptidase subunit of the of the 26S proteasome. [Date last reviewed: 2019-09-26]
Gene Group (FlyBase)
PROTEASOME ENDOPEPTIDASES -
The proteasome is a threonine protease in which the active-site threonine residue is at the N-terminus of the beta subunits. Only three of the seven eukaryotic beta subunits (beta1, beta2 and beta5) retain an intact active site. These subunits are activated following autocatalytic processing of an N-terminal propeptide - propeptide autocleavage and substrate proteolysis utilize closely related mechanisms. (Adapted from PMID:28583440.)
26S PROTEASOME -
The 26S proteasome degrades polyubiquitinated proteins in the cytoplasm and nucleus. It is formed from a cylindrical catalytic core 20S proteasome capped at each end by a regulatory 19S particle. The 19S regulatory particle recognizes, unfolds, and translocates ubiquitinated proteins into the catalytic core particle in an ATP-dependent manner. (Adapted from FBrf0215459).
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Prosβ5 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q7K148)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.49

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Prosβ5-RA
FBtr0088225
NM_143757
1139
282
Prosβ5-RB
FBtr0088224
NM_165801
1076
282
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Prosβ5-PA
FBpp0087320
31.2
282
6.94
Q7K148
NP_652014
AAF58748
Prosβ5-PB
FBpp0087319
31.2
282
6.94
Q7K148
NP_724974
AAM68751
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

282 aa isoforms: Prosβ5-PA, Prosβ5-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Prosβ5 using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.88

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 7 -- 8
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 9 -- 10
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 11 -- 12
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 13 -- 16
organism | ubiquitous
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytosol
inferred from high throughput direct assay
(Lye et al., 2014)
located_in nucleoplasm
inferred from high throughput direct assay
(Lye et al., 2014)
part_of proteasome complex
inferred from direct assay
(Nickell et al., 2009)
part_of proteasome core complex
inferred from direct assay
(Sumegi et al., 2003)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Prosβ5 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 7 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Prosβ5
Transgenic constructs containing regulatory region of Prosβ5
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation ( 1 )
Gene disrupted in
Df(2R)Exel7112
(Ryder, 2004.4.26)
Inferred from location ( 7 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal, with Scer\GAL4insc-Mz1407
lethal, with Scer\GAL4pnr-MD237
lethal - all die during P-stage, with Scer\GAL4Mef2.PR
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4tin.CΔ4
Other Phenotypes
Allele
abnormal feeding behavior | adult stage | RU486 conditional, with Scer\GAL4da.Switch.PT
abnormal neuroanatomy | larval stage, with Scer\GAL4insc-Mz1407
abnormal neuroanatomy | third instar larval stage, with Scer\GAL4Gli.PU
abnormal starvation stress response | adult stage | RU486 conditional, with Scer\GAL4da.Switch.PT
long lived | RU486 conditional, with Scer\GAL4da.Switch.PT
short lived | heat sensitive, with Scer\GAL4tin.CΔ4
Phenotype manifest in
Allele
abdominal nerve | third instar larval stage, with Scer\GAL4Gli.PU
actin cytoskeleton | adult stage | female, with Scer\GAL4VP16.mat.αTub67C
egg chamber, with Scer\GAL4VP16.mat.αTub67C
embryonic/larval fat body | third instar larval stage, with Scer\GAL4Cg.PA
filamentous actin | oogenesis, with Scer\GAL4VP16.mat.αTub67C
ganglion mother cell | larval stage, with Scer\GAL4insc-Mz1407
larval neuroblast | larval stage, with Scer\GAL4insc-Mz1407
mitochondrion | third instar larval stage, with Scer\GAL4Cg.PA
nurse cell ring canal, with Scer\GAL4VP16.mat.αTub67C
subperineurial glial cell | third instar larval stage, with Scer\GAL4Gli.PU
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (19)
Hsap\PSMB5
13 of 14
Yes
Yes
Hsap\PSMB8
11 of 14
No
Yes
Hsap\PSMB11
5 of 14
No
Yes
Hsap\PSMA5
2 of 14
No
No
1  
Hsap\PSMA7
2 of 14
No
No
Hsap\PSMB1
2 of 14
No
No
1  
Hsap\PSMB2
2 of 14
No
No
Hsap\PSMB3
2 of 14
No
No
Hsap\PSMB7
2 of 14
No
No
Hsap\PSMB10
2 of 14
No
No
Hsap\PSMA1
1 of 14
No
No
Hsap\PSMA2
1 of 14
No
No
1  
Hsap\PSMA3
1 of 14
No
No
Hsap\PSMA4
1 of 14
No
No
Hsap\PSMA6
1 of 14
No
No
Hsap\PSMA8
1 of 14
No
No
Hsap\PSMB4
1 of 14
No
No
Hsap\PSMB6
1 of 14
No
No
Hsap\PSMB9
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (19)
Rnor\Psmb5
13 of 14
Yes
Yes
Rnor\Psmb8
11 of 14
No
Yes
Rnor\Psmb11
4 of 14
No
Yes
Rnor\Psmb1
2 of 14
No
No
Rnor\Psmb2
2 of 14
No
No
Rnor\Psmb10
2 of 14
No
No
Rnor\LOC100360846
1 of 14
No
No
Rnor\Psma1
1 of 14
No
No
Rnor\Psma3
1 of 14
No
No
Rnor\Psma4
1 of 14
No
No
Rnor\Psma5
1 of 14
No
No
Rnor\Psma6
1 of 14
No
No
Rnor\Psma8
1 of 14
No
No
Rnor\Psmb3
1 of 14
No
No
Rnor\Psmb4
1 of 14
No
No
Rnor\Psmb6
1 of 14
No
No
Rnor\Psmb7
1 of 14
No
No
Rnor\Psmb9
1 of 14
No
No
Rnor\RGD1564425
1 of 14
No
No
Mus musculus (laboratory mouse) (18)
Mmus\Psmb5
12 of 14
Yes
Yes
Mmus\Psmb8
11 of 14
No
Yes
Mmus\Psmb11
5 of 14
No
Yes
Mmus\Psmb1
2 of 14
No
No
Mmus\Psmb2
2 of 14
No
No
Mmus\Psmb3
2 of 14
No
No
Mmus\Psmb7
2 of 14
No
No
Mmus\Psmb10
2 of 14
No
No
Mmus\Psma1
1 of 14
No
No
Mmus\Psma3
1 of 14
No
No
Mmus\Psma4
1 of 14
No
No
Mmus\Psma5
1 of 14
No
No
Mmus\Psma6
1 of 14
No
No
Mmus\Psma7
1 of 14
No
No
Mmus\Psma8
1 of 14
No
No
Mmus\Psmb4
1 of 14
No
No
Mmus\Psmb6
1 of 14
No
No
Mmus\Psmb9
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (8)
Xtro\psmb5
11 of 13
Yes
Yes
Xtro\LOC100485711
3 of 13
No
Yes
Xtro\psmb8
3 of 13
No
Yes
Xtro\ctsv
1 of 13
No
No
Xtro\panx2
1 of 13
No
No
Xtro\psma5
1 of 13
No
No
Xtro\psmb1
1 of 13
No
No
Xtro\psmb11
1 of 13
No
No
Danio rerio (Zebrafish) (21)
Drer\psmb5
13 of 14
Yes
Yes
Drer\psmb8a
10 of 14
No
Yes
Drer\psmb11b
4 of 14
No
Yes
Drer\psmb11a
3 of 14
No
Yes
Drer\psmb1
2 of 14
No
No
Drer\psmb2
2 of 14
No
No
Drer\psmb3
2 of 14
No
No
Drer\psmb7
2 of 14
No
No
Drer\psmb10
2 of 14
No
No
Drer\psmb13a
2 of 14
No
No
Drer\psma3
1 of 14
No
No
Drer\psma4
1 of 14
No
No
Drer\psma5
1 of 14
No
No
Drer\psma6a
1 of 14
No
No
Drer\psma6b
1 of 14
No
No
Drer\psma6l
1 of 14
No
No
Drer\psma8
1 of 14
No
No
Drer\psmb4
1 of 14
No
No
Drer\psmb6
1 of 14
No
No
Drer\psmb9a
1 of 14
No
No
Drer\psmb12
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (13)
Cele\pbs-5
14 of 14
Yes
Yes
Cele\pas-4
2 of 14
No
No
Cele\pas-5
2 of 14
No
No
Cele\pbs-2
2 of 14
No
No
Cele\pbs-3
2 of 14
No
No
Cele\pbs-4
2 of 14
No
No
Cele\pbs-6
2 of 14
No
No
Cele\pas-1
1 of 14
No
No
Cele\pas-3
1 of 14
No
No
Cele\pas-6
1 of 14
No
No
Cele\pas-7
1 of 14
No
No
Cele\pbs-1
1 of 14
No
No
Cele\pbs-7
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (14)
Agam\AgaP_AGAP010718
12 of 12
Yes
Yes
Agam\AgaP_AGAP004991
2 of 12
No
No
Agam\AgaP_AGAP005423
2 of 12
No
No
Agam\AgaP_AGAP008816
2 of 12
No
No
Agam\AgaP_AGAP008837
2 of 12
No
No
Agam\AgaP_AGAP010253
2 of 12
No
No
Agam\AgaP_AGAP011423
2 of 12
No
No
Agam\AgaP_AGAP001697
1 of 12
No
No
Agam\AgaP_AGAP001995
1 of 12
No
No
Agam\AgaP_AGAP003935
1 of 12
No
No
Agam\AgaP_AGAP004960
1 of 12
No
No
Agam\AgaP_AGAP009271
1 of 12
No
No
Agam\AgaP_AGAP011444
1 of 12
No
No
Agam\AgaP_AGAP012608
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (22)
Atha\AT3G26340
13 of 13
Yes
Yes
Atha\PBE1
13 of 13
Yes
Yes
Atha\PAC1
2 of 13
No
No
Atha\PAD2
2 of 13
No
No
Atha\PAF2
2 of 13
No
No
Atha\PAG1
2 of 13
No
No
Atha\PBB1
2 of 13
No
No
Atha\PBB2
2 of 13
No
No
Atha\PBC1
2 of 13
No
No
Atha\PBC2
2 of 13
No
No
Atha\PBD1
2 of 13
No
No
Atha\PBD2
2 of 13
No
No
Atha\PBF1
2 of 13
No
No
Atha\AT4G15165
1 of 13
No
No
Atha\PAA1
1 of 13
No
No
Atha\PAA2
1 of 13
No
No
Atha\PAD1
1 of 13
No
No
Atha\PAE1
1 of 13
No
No
Atha\PAE2
1 of 13
No
No
Atha\PAF1
1 of 13
No
No
Atha\PBA1
1 of 13
No
No
Atha\PBG1
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (14)
Scer\PRE2
12 of 13
Yes
Yes
Scer\PRE1
2 of 13
No
No
Scer\PRE6
2 of 13
No
No
Scer\PRE7
2 of 13
No
No
Scer\PRE9
2 of 13
No
No
Scer\PUP1
2 of 13
No
No
Scer\PUP2
2 of 13
No
No
Scer\PUP3
2 of 13
No
No
Scer\PRE3
1 of 13
No
No
Scer\PRE4
1 of 13
No
No
Scer\PRE5
1 of 13
No
No
Scer\PRE8
1 of 13
No
No
Scer\PRE10
1 of 13
No
No
Scer\SCL1
1 of 13
No
No
Schizosaccharomyces pombe (Fission yeast) (13)
Spom\pts1
11 of 12
Yes
Yes
Spom\pam1
2 of 12
No
No
Spom\pup1
2 of 12
No
No
Spom\pup2
2 of 12
No
No
Spom\pup3
2 of 12
No
No
Spom\SPAC31A2.04c
2 of 12
No
No
Spom\pre3
1 of 12
No
No
Spom\pre6
1 of 12
No
No
Spom\pre10
1 of 12
No
No
Spom\SPAC6G10.04c
1 of 12
No
No
Spom\SPAC13C5.01c
1 of 12
No
No
Spom\SPBC577.10
1 of 12
No
No
Spom\SPBC646.16
1 of 12
No
No
Escherichia coli (enterobacterium) (1)
Ecol\hslV
1 of 11
Yes
No
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Prosβ5. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (24)
Prosβ5R1
11 of 13
Prosβ5R2
11 of 13
Prosβ2
5 of 13
Prosβ2R1
5 of 13
Prosβ2R2
5 of 13
Prosα1
4 of 13
Prosα1R
4 of 13
Prosα3
4 of 13
Prosα4T2
4 of 13
Prosα5
4 of 13
Prosα6
4 of 13
Prosα6T
4 of 13
Prosα7
4 of 13
Prosβ1
4 of 13
Prosβ3
4 of 13
Prosβ4
4 of 13
Prosβ4R1
4 of 13
Prosβ4R2
4 of 13
Prosβ6
4 of 13
Prosα2
3 of 13
Prosα3T
3 of 13
Prosα4
3 of 13
Prosα4T1
3 of 13
Prosβ7
3 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Evidence
    References
    Prosβ5HMS00119
    model of  neurodegenerative disease
    CEA
    (Fernández-Cruz et al., 2020)
    Potential Models Based on Orthology ( 1 )
    Modifiers Based on Experimental Evidence ( 3 )
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    PSMB5; proteasome 20S subunit beta 5
    13 of 14
    600306
        PSMB8; proteasome 20S subunit beta 8
        11 of 14
        177046
        PSMB11; proteasome subunit beta 11
        5 of 14
        611137
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            Interaction Browsers
            View in FlyBase Interactions Browser   View in MIST tool (external link)
            Please see the Physical Interaction reports below for full details
            protein-protein
            Physical Interaction
            Assay
            References
            Prosβ5 - AnxB10
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - CG11885
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - CG12321
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - CG13319
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - CG15717
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - Dera
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - Eno
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - Klc
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - Mdh2
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - Moe
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - PSMG1
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - Pomp
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - Prosα1
            experimental knowledge based, anti bait coimmunoprecipitation, western blot
            (Tsakiri et al., 2013, Tsakiri et al., 2013, Guruharsha et al., 2011)
            Prosβ5 - Prosα2
            anti bait coimmunoprecipitation, western blot, experimental knowledge based
            (Tsakiri et al., 2019, Tsakiri et al., 2013, Tsakiri et al., 2013, Guruharsha et al., 2011)
            Prosβ5 - Prosα3
            anti bait coimmunoprecipitation, western blot, experimental knowledge based
            (Tsakiri et al., 2013, Tsakiri et al., 2013, Guruharsha et al., 2011)
            Prosβ5 - Prosα4
            anti bait coimmunoprecipitation, western blot, experimental knowledge based
            (Tsakiri et al., 2013, Tsakiri et al., 2013, Guruharsha et al., 2011)
            Prosβ5 - Prosα5
            anti bait coimmunoprecipitation, western blot, experimental knowledge based
            (Tsakiri et al., 2013, Tsakiri et al., 2013, Guruharsha et al., 2011)
            Prosβ5 - Prosα6
            anti bait coimmunoprecipitation, western blot, experimental knowledge based
            (Tsakiri et al., 2013, Tsakiri et al., 2013, Guruharsha et al., 2011)
            Prosβ5 - Prosα7
            experimental knowledge based, anti bait coimmunoprecipitation, western blot
            (Tsakiri et al., 2013, Tsakiri et al., 2013, Guruharsha et al., 2011)
            Prosβ5 - Prosβ1
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - Prosβ2
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - Prosβ3
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - Prosβ4
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - Prosβ6
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - Prosβ7
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - Prx6c
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - REG
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - Rpn1
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - Rpn10
            cosedimentation through density gradient, western blot, anti bait coimmunoprecipitation, anti tag western blot
            (Kreko-Pierce and Eaton, 2017)
            Prosβ5 - Rpn11
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - Rpn13
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - Rpn2
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - Rpn6
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - Rpn7
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - Rpn8
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - Rpn9
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - Rpt1
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - Rpt2
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - Rpt3
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - Rpt4
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - Rpt5
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - Rpt6
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - Sh3β
            experimental knowledge based
            (Guruharsha et al., 2011)
            Prosβ5 - ctp
            anti bait coimmunoprecipitation, western blot
            (Kreko-Pierce and Eaton, 2017)
            Prosβ5 - scu
            experimental knowledge based
            (Guruharsha et al., 2011)
            Summary of Genetic Interactions
            Interaction Browsers
            View in FlyBase Interactions Browser   View in MIST tool (external link)
            Please look at the allele data for full details of the genetic interactions
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Prosβ5
            enhanceable
            hts
            (Hudson et al., 2019)
            Prosβ5
            suppressible
            hts
            (Hudson et al., 2019)
            Prosβ5
            enhanceable
            kel
            (Hudson et al., 2015)
            hts|Prosβ5
            enhanceable
            kel
            (Hudson et al., 2019)
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            External Data
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            DroID - A comprehensive database of gene and protein interactions.
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Pathways
            Signaling Pathways (FlyBase)
            Metabolic Pathways
            FlyBase
            External Links
            Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.
            External Data
            Linkouts
            KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            Class of Gene
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            2R
            Recombination map
            2-63
            Cytogenetic map
            47C1-47C1
            Sequence location
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            47C1-47C1
            Limits computationally determined from genome sequence between P{lacW}l(2)k00909k00909&P{EP}EP2619EP2619 and P{lacW}l(2)k15826k15826&P{EP}EP471
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            47B-47C
            (Renault, 1999.12.20)
            Experimentally Determined Recombination Data
            Location

            2-63

            (FlyBase, 2016)

            2-62.3

            (Comeron et al., 2012)
            Left of (cM)
            Right of (cM)
            Notes
            Stocks and Reagents
            Stocks (12)
            Genomic Clones (18)
             

            Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

            cDNA Clones (95)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequenced
            BDGP DGC clones
            Other clones
            Drosophila Genomics Resource Center cDNA clones

            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            RNAi and Array Information
            Linkouts
            DRSC - Results frm RNAi screens
            Antibody Information
            Laboratory Generated Antibodies
             
            Commercially Available Antibodies
             
            Cell Line Information
            Publicly Available Cell Lines
             
              Other Stable Cell Lines
               
                Other Comments

                RNAi screen using dsRNA made from templates generated with primers directed against this gene results in the formation of short, monastral spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

                (Goshima et al., 2007)

                RNAi screen using dsRNA made from templates generated with primers directed against this gene in S2 cells does not affect the number of prophase centrosomes, but monastral bipolar spindles are formed during mitosis, suggesting that the centrosomes are fused.

                (Goshima et al., 2007)

                RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.

                (Boutros et al., 2004)

                Area matching Drosophila ESTs AI064230 and AA263288.

                (Davies, 2001.3.15)
                Relationship to Other Genes
                Source for database merge of

                Source for merge of: Prosβ5 CG12323

                (Bayraktaroglu, 2000.8.7)

                Source for merge of: Prosβ5 anon-WO0118547.116

                (FlyBase, 1996-)
                Additional comments

                FlyBase curator comment: "EP2619" overexpression phenotype stated to be due to its effect on EST LD08717 (which corresponds to Prosβ5) but orientation of the P{EP} element suggests otherwise.

                (Pena-Rangel et al., 2002)

                Source for merge of Prosβ5 anon-WO0118547.116 was sequence comparison ( date:051113 ).

                (FlyBase, 1996-)
                Nomenclature History
                Source for database identify of
                Nomenclature comments

                The nomenclature of genes encoding subunits of the 26S proteasome of D. melanogaster have been standardized according to FBrf0215459. These symbols/names largely follow those used already in FlyBase, and largely mirror fly community usage. HOWEVER, note that at least one other nomenclature system exists that is followed by the HUGO Gene Nomenclature Committee (HGNC), for example, with the unfortunate result that several D. melanogaster genes have shared synonyms.

                (FlyBase, 2009-)
                Etymology
                Synonyms and Secondary IDs (19)
                Reported As
                Symbol Synonym
                Beta 5 subunit
                (Pomatto et al., 2020)
                Beta-5
                (Lundgren et al., 2005)
                CG12323
                (National Institute of Genetics Fly Stocks, 2022-, Nguyen et al., 2019, Caizzi et al., 2016, Çiçek et al., 2016, Hosono et al., 2015, Wang et al., 2014, Schertel et al., 2013, Yano et al., 2012, Cammarato et al., 2011, Ni et al., 2011.7.19, Belote and Zhong, 2009, Keleman et al., 2009.8.5, Barbash and Lorigan, 2007, Gandhi et al., 2007, Goshima et al., 2007, Juhász et al., 2007, Molnar et al., 2006, Lundgren et al., 2005, Ma et al., 2002, Rawlings, 2001.2.12)
                LD08717
                (Pena-Rangel et al., 2002)
                PSMB5
                (Lindquist et al., 2011)
                Pros b5
                (Molnar et al., 2006)
                Prosbeta5
                (Kang et al., 2023, Kong et al., 2022, Ribot et al., 2022, Pang et al., 2021, Gene Disruption Project members, 2018-, Yu et al., 2016, Zirin et al., 2015, Schertel et al., 2013, Gurudatta et al., 2012, Lindquist et al., 2011, Neumüller et al., 2011, Carbone et al., 2009, Zhou et al., 2008, Barbash and Lorigan, 2007, Lundgren et al., 2005, Stanyon et al., 2004, Giot et al., 2003)
                Prosβ5
                (Lee et al., 2024, Chocron et al., 2022, Kumar and Baker, 2022, Järvelä-Stölting et al., 2021, Manola et al., 2021, Rai et al., 2021, Walls et al., 2020, Gumeni et al., 2019, Hudson et al., 2019, Manola et al., 2019, Munkácsy et al., 2019, Nikou et al., 2019, Sato et al., 2019, Tsakiri et al., 2019, Zhou et al., 2019, Langerak et al., 2018, Liu et al., 2018, Kreko-Pierce and Eaton, 2017, Hudson et al., 2015, Sanhueza et al., 2015, Rumpf et al., 2014, Lőw et al., 2013, Velentzas et al., 2013, Goshima et al., 2007, Zhang et al., 2007, Bandyopadhyay et al., 2006, Ma et al., 2002)
                anon-WO0118547.116
                beta-5
                (Goshima et al., 2007)
                beta5
                (Yano et al., 2012)
                dbeta5
                (Velentzas et al., 2013)
                dβ5
                (Vernace et al., 2007, Wojcik and DeMartino, 2002)
                prosbeta5
                (Blount et al., 2018)
                prosβ5
                (Hirschhäuser et al., 2021)
                β-5
                (Wang et al., 2014)
                β5
                (Sun et al., 2025, Velentzas et al., 2020, Nguyen et al., 2019, Tsakiri et al., 2017, Tsakiri et al., 2013, Velentzas et al., 2013, Belote and Zhong, 2009)
                Name Synonyms
                20S proteasome
                (Bohm et al., 2000)
                Prosbeta5
                (Cammarato et al., 2011)
                Prosβ5
                Proteasome β5 subunit
                β5
                (Nickell et al., 2009)
                Secondary FlyBase IDs
                • FBgn0033553
                • FBgn0062234
                Datasets (0)
                Study focus (0)
                Experimental Role
                Project
                Project Type
                Title
                Study result (0)
                Result
                Result Type
                Title
                External Crossreferences and Linkouts ( 98 )
                Sequence Crossreferences
                NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                Other crossreferences
                AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                FlyMine - An integrated database for Drosophila genomics
                InterPro - A database of protein families, domains and functional sites
                KEGG Genes - Molecular building blocks of life in the genomic space.
                MARRVEL_MODEL - MARRVEL (model organism gene)
                Linkouts
                BioGRID - A database of protein and genetic interactions.
                Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                DroID - A comprehensive database of gene and protein interactions.
                DRSC - Results frm RNAi screens
                FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                Flygut - An atlas of the Drosophila adult midgut
                FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
                iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                MIST (protein-protein) - An integrated Molecular Interaction Database
                Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.
                References (127)