FlyBase Gene Report: Dmel\CG7483

General Information

Symbol

Dmel\CG7483

Species

D. melanogaster

Name

Annotation Symbol

CG7483

Feature Type

protein_coding_gene

FlyBase ID

FBgn0037573

Gene Model Status

Current

Stock Availability

18 publicly available

Enzyme Name (EC)

RNA helicase (3.6.4.13)

Gene Summary

ATP-dependent RNA helicase (PubMed:22961380). Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs (PubMed:14973490, PubMed:22961380). Involved in exon definition of genes containing long introns, including the rolled/MAPK gene (PubMed:20946982, PubMed:20946983). Has a role in oskar mRNA localization at the posterior pole of the developing oocyte (PubMed:14973490). (UniProt, Q9VHS8)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

eIF4AIII, eIF4A3, eIF4A, eIF4A-III, l(3)84Fi

Key Links

Genomic Location

Cytogenetic map

84F11-84F11

Sequence location

3R:8,313,208..8,314,864 [+]

Recombination map

3-48

RefSeq locus

NT_033777 REGION:8313208..8314864

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (16 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with UniProtKB:Q9VJ87

(Barbosa et al., 2012)

inferred from physical interaction with FLYBASE:btz; FB:FBgn0045862

(Palacios et al., 2004)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR011545

(InterPro Project Members, 2004-)

enables mRNA binding

inferred from biological aspect of ancestor with PANTHER:PTN002776867

(GO Reference Genome Project, 2011-)

enables nucleic acid binding

inferred from electronic annotation with InterPro:IPR011545

(InterPro Project Members, 2004-)

enables RNA helicase activity

inferred from electronic annotation with InterPro:IPR014014

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN002774595

(GO Reference Genome Project, 2011-)

Biological Process (6 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

involved_in chromatin organization

inferred from mutant phenotype

(Klusza et al., 2013)

involved_in exon-exon junction complex assembly

inferred from mutant phenotype

(Zhang and Krainer, 2007)

involved_in pole plasm oskar mRNA localization

inferred from genetic interaction with FLYBASE:btz; FB:FBgn0045862

(Palacios et al., 2004)

involved_in positive regulation of gene expression

inferred from mutant phenotype

(Ashton-Beaucage et al., 2014)

involved_in RNA splicing

inferred from mutant phenotype

(Ashton-Beaucage et al., 2010)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in mRNA splicing, via spliceosome

inferred by curator from GO:0071011,GO:0071013

(Herold et al., 2009)

inferred from biological aspect of ancestor with PANTHER:PTN002777794

(GO Reference Genome Project, 2011-)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

part_of catalytic step 2 spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

located_in nucleus

inferred from direct assay

(Palacios et al., 2004)

located_in pole plasm

inferred from direct assay

(Palacios et al., 2004)

part_of precatalytic spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

part_of catalytic step 2 spliceosome

inferred from biological aspect of ancestor with PANTHER:PTN002777794

(GO Reference Genome Project, 2011-)

is_active_in nucleolus

inferred from biological aspect of ancestor with PANTHER:PTN002777794

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

SPLICEOSOME COMPLEX C

SPLICEOSOME COMPLEX P

DEAD-BOX RNA HELICASES

Protein Family (UniProt)

Belongs to the DEAD box helicase family. (Q9VHS8)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

RNA helicase activity

ATP + H2O = ADP + phosphate + H(+) (3.6.4.13)

Summaries

Gene Group (FlyBase)

SPLICEOSOME COMPLEX C -: Nuclear pre-mRNA splicing is catalyzed by the spliceosome which assembles in a step-wise manner. Complex B undergoes a number of structural rearrangements and U4 and U1 snRNPs dissociate to generate complex C, the catalytic spliceosome. (Adapted from PMID:24452469 and PMID:23118483).
SPLICEOSOME COMPLEX P -: Nuclear pre-mRNA splicing is catalyzed by the spliceosome which assembles in a step-wise manner. Complex P, the post-spliceosomal complex, is formed after the catalytic removal of the intron by complex C. Complex P contains the lariat intron and spliced exons. (Adapted from PMID:24452469 and PMID:23118483).
DEAD-BOX RNA HELICASES -: DEAD-box RNA helicases belong to helicase superfamily 2 (SF2). They are characterized by the presence of nine conserved helicase motifs, including the DEAD sequence in motif II. (Adapted from PMID:21509200.)

Protein Function (UniProtKB)

(UniProt, Q9VHS8)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CG7483 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VHS8)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.49

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CG7483-RA

FBtr0081834

NM_141531

1481

399

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

CG7483-PA

FBpp0081324

45.6

399

5.82

Q9VHS8

NP_649788

AAF54221

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Part of the mRNA splicing-dependent exon junction complex (EJC) complex; the core complex contains btz/CASC3, eIF4AIII, mago and tsu/RBM8A (PubMed:14973490, PubMed:22961380). Interacts with btz/CASC3 and mago (PubMed:14973490). Interacts with ncm/CWC22 (PubMed:22961380).

(UniProt, Q9VHS8)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VHS8

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG7483 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

eIF4AIII_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.54

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of catalytic step 2 spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

located_in nucleus

inferred from direct assay

(Palacios et al., 2004)

located_in pole plasm

inferred from direct assay

(Palacios et al., 2004)

part_of precatalytic spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\CG7483 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG7483

View exonic expression by tissue for Dmel\CG7483

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0037573

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40987/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0037573

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0037573

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0037573

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7483

Flygut - An atlas of the Drosophila adult midgut

FBgn0037573

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 13 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG7483

Transgenic constructs containing regulatory region of CG7483

Aberrations (Deficiencies and Duplications) ( 20 )

Inferred from experimentation ( 20 )

Gene disrupted in

Df(3R)D6

(Baker et al., 1991)

Df(3R)dsx2D

(Baker et al., 1991)

Df(3R)dsx11

(Baker et al., 1991)

Df(3R)ED5230

(Marygold et al., 2007)

Df(3R)ED5296

(Marygold et al., 2007)

Df(3R)Exel6147

(Marygold et al., 2007, Ryder, 2004.4.26)

Df(3R)p13

(Baker et al., 1991)

Df(3R)p30

(Baker et al., 1991)

Df(3R)p40

(Baker et al., 1991)

Gene not disrupted in

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

CG7483¹⁹

lethal | germline clone

CG7483¹⁹

Other Phenotypes

Allele

increased cell death | germline clone

CG7483¹⁹

Phenotype manifest in

Allele

chromatin, with Scer\GAL4^{VP16.mat.αTub67C}

CG7483^HMS00442

cytoskeleton & oocyte | germ-line clone

CG7483¹⁹

egg chamber, with Scer\GAL4^{VP16.mat.αTub67C}

CG7483^HMS00442

main body follicle cell | germline clone

CG7483¹⁹

nucleus & oocyte | germ-line clone

CG7483¹⁹

nurse cell, with Scer\GAL4^{VP16.mat.αTub67C}

CG7483^HMS00442

ovary, with Scer\GAL4^nanos.PG

ovary, with Scer\GAL4^NP1624

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (37)

Hsap\EIF4A3

13 of 14

Yes

5 of 14

5 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (23)

Rnor\Eif4a3

14 of 14

Yes

5 of 14

5 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (28)

Mmus\Eif4a3

14 of 14

Yes

Mmus\Eif4a3l1

11 of 14

Yes

Mmus\Eif4a3l2

11 of 14

Yes

5 of 14

5 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (17)

Xtro\eif4a3.1

11 of 13

Yes

Xtro\eif4a1

3 of 13

Xtro\eif4a2

3 of 13

Xtro\apoc1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (25)

Drer\eif4a3

13 of 14

Yes

5 of 14

5 of 14

5 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (26)

Cele\F33D11.10

14 of 14

Yes

Cele\Y65B4A.6

14 of 14

Yes

5 of 14

4 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (29)

Agam\AgaP_AGAP003089

12 of 12

Yes

5 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (52)

Atha\EIF4A-III

13 of 13

Yes

Atha\AT1G51380

9 of 13

Yes

5 of 13

5 of 13

5 of 13

2 of 13

Yes

Atha\PMH1

2 of 13

Yes

Atha\PMH2

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (38)

Scer\FAL1

13 of 13

Yes

7 of 13

7 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Scer\YEL077C

1 of 13

Yes

Scer\YFL066C

1 of 13

Yes

Scer\YHL050C

1 of 13

Yes

Scer\YHR218W

1 of 13

Yes

Scer\YIL177C

1 of 13

Yes

Scer\YJL225C

1 of 13

Yes

Scer\YLL066C

1 of 13

Yes

Scer\YLL067C

1 of 13

Yes

Scer\YML133C

1 of 13

Yes

Scer\YPR204W

1 of 13

Yes

Scer\YRF1-1

1 of 13

Yes

Scer\YRF1-2

1 of 13

Yes

Scer\YRF1-3

1 of 13

Yes

Scer\YRF1-4

1 of 13

Yes

Scer\YRF1-5

1 of 13

Yes

Scer\YRF1-6

1 of 13

Yes

Scer\YRF1-7

1 of 13

Yes

Scer\YRF1-8

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (13)

Spom\SPAC1F5.10

12 of 12

Yes

4 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (3)

Ecol\deaD

6 of 11

Yes

Ecol\dbpA

3 of 11

Yes

Ecol\rhlE

3 of 11

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:CG7483. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (33)

eIF4A

8 of 13

Gem3

4 of 13

me31B

4 of 13

abs

3 of 13

bel

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

vas

3 of 13

ais

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

pit

2 of 13

Prp5

2 of 13

pths

2 of 13

Rs1

2 of 13

Blm

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

CG7483^HMS00696

exacerbates cancer

modeled by Rbf^HMS03004

(Parkhitko et al., 2021)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

EIF4A3; eukaryotic translation initiation factor 4A3

13 of 14

608546

ROBIN SEQUENCE WITH CLEFT MANDIBLE AND LIMB ANOMALIES

EIF4A1; eukaryotic translation initiation factor 4A1

5 of 14

602641

EIF4A2; eukaryotic translation initiation factor 4A2

5 of 14

EUKARYOTIC TRANSLATION INITIATION FACTOR 4A, ISOFORM 2; EIF4A2

NEURODEVELOPMENTAL DISORDER WITH HYPOTONIA AND SPEECH DELAY, WITH OR WITHOUT SEIZURES; NEDHSS

neurodevelopmental disorder with hypotonia and speech delay

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

CG7483 - CG8353

experimental knowledge based

(Guruharsha et al., 2011)

CG7483 - Polr2A

anti bait coimmunoprecipitation, western blot

(Singh et al., 2019)

CG7483 - btz

anti tag coimmunoprecipitation, anti tag western blot, two hybrid, Identification by mass spectrometry

(Ho et al., 2022, Bansal et al., 2020, Palacios et al., 2004)

CG7483 - hfp

anti tag coimmunoprecipitation, Identification by mass spectrometry, anti tag western blot

(Bansal et al., 2020)

CG7483 - mago

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, pull down, molecular weight estimation by staining

(Obrdlik et al., 2019, Ghosh et al., 2014, Palacios et al., 2004)

CG7483 - ncm

anti tag coimmunoprecipitation, Identification by mass spectrometry, anti tag western blot, affinity technology

(Bansal et al., 2020, Barbosa et al., 2012)

CG7483 - tsu

anti tag coimmunoprecipitation, western blot, pull down, molecular weight estimation by staining, anti bait coimmunoprecipitation

(Obrdlik et al., 2019, Ghosh et al., 2014, Palacios et al., 2004)

RNA-protein

Physical Interaction

Assay

References

CG7483 - osk

anti tag coimmunoprecipitation, autoradiography

(Ghosh et al., 2012)

CG7483 - sp3

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Saulière et al., 2010)

CG7483 - su(w^a)

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Saulière et al., 2010)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

btz

enhanceable

CG7483

(Palacios et al., 2004)

osk|btz

enhanceable

CG7483

(Palacios et al., 2004)

Ras85D

suppressible

CG7483

(Ashton-Beaucage et al., 2010)

External Data

Subunit Structure (UniProtKB)

(UniProt, Q9VHS8 )

Linkouts

BioGRID - A database of protein and genetic interactions.

66168

DroID - A comprehensive database of gene and protein interactions.

FBgn0037573

MIST (genetic) - An integrated Molecular Interaction Database

40987

MIST (protein-protein) - An integrated Molecular Interaction Database

40987

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-48

Cytogenetic map

84F11-84F11

Sequence location

3R:8,313,208..8,314,864 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

84F11-84F11

Limits computationally determined from genome sequence between P{PZ}Mcm2^rL074 and P{EP}EP833

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-48

(FlyBase, 2016)

3-44.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (18)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

67695

w^*; P{αTub67C-CG7483.GFP}2/CyO

20978

w¹¹¹⁸; P{GT1}CG7483^GT-000230

67694

w^*; P{αTub67C-CG7483.GFP}3/TM3, Sb¹

94911

w^*; P{CG7483.HA.FLAG}attP3/TM3, Sb¹

2781

CG7483¹⁹ red¹ e⁴/TM3, Sb¹

67696

w^*; P{neoFRT}82B CG7483¹⁹ e⁴/TM3, Sb¹

FlyORF

F001353

M{UAS-CG7483.ORF}ZH-86Fb

F001132

M{UAS-CG7483.ORF.3xHA}ZH-86Fb

Kyoto

107466

CG7483¹⁹ red¹ e⁴/TM3, Sb¹

VDRC

v330257

P{VSH330257}attP40

v108580

P{KK101462}VIE-260B

v318766

PBac{fTRG01134.sfGFP-TVPTBF}VK00002

More stocks available...

Genomic Clones (15)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (74)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE50350

Other clones

FI20117

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0037573

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0037573

Antibody Information

Laboratory Generated Antibodies

monoclonal

(Na et al., 2016)

polyclonal

(Choudhury et al., 2016)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

eIF4AIII has a role in the splicing regulation of genes containing long introns.

(Ashton-Beaucage et al., 2010)

Area matching Drosophila EST AA817394. This EST has sequence similarity to Dead box DNA helicase genes.

(Davies, 2001.3.15)

Relationship to Other Genes

Source for database merge of

Source for merge of: eIF4AIII anon-WO0118547.364

(FlyBase, 1996-)

Additional comments

Source for merge of eIF4AIII anon-WO0118547.364 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: CG7483 eIF4AIII

(Marygold et al., 2017)

Nomenclature comments

FlyBase curator comment: Reverted this gene symbol (eIF4AIII) to its CG designation (CG7483) as it has no known translation initiation factor activity - rather, it is a core component of exon junction complex (see FBrf0174990).

(Marygold et al., 2017)

Etymology

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

7483

(Ravi et al., 2009)

DmRH28

(Boudet et al., 2001)

EIF4AIII

(Czech et al., 2013, Ashton-Beaucage and Therrien, 2011, Friedman et al., 2011)

anon-WO0118547.364

eIF4A

(Kim et al., 2021, Lasko, 2000)

eIF4A-III

(Vasudevan et al., 2020, Hernandez and Vazquez-Pianzola, 2005)

eIF4A3

(Morillo et al., 2023, Gao et al., 2019, Zhang and Krainer, 2007)

eIF4AII

(Okuda et al., 2022)

eIF4AIII

(Dyson et al., 2025, Joseph and Lai, 2021, Parkhitko et al., 2021, Bansal et al., 2020, Obrdlik et al., 2019, Singh et al., 2019, Trcek and Lehmann, 2019, Wang et al., 2018, Ashton-Beaucage and Therrien, 2017, Garcia-Garcia et al., 2017, Ashton-Beaucage et al., 2016, Choudhury et al., 2016, Jakšić and Schlötterer, 2016, Liu et al., 2016, Na et al., 2016, Sarov et al., 2016, Ashton-Beaucage et al., 2014, Ghosh et al., 2014, Hayashi et al., 2014, White-Grindley et al., 2014, Handler et al., 2013, Hernández et al., 2013, Klusza et al., 2013, Schertel et al., 2013, Barbosa et al., 2012, Ghosh et al., 2012, Cheng et al., 2011, Liu et al., 2011, Ashton-Beaucage et al., 2010, Roignant and Treisman, 2010, Saulière et al., 2010, Ulvila et al., 2010, Herold et al., 2009, Stielow et al., 2008, Marygold et al., 2007)

eIF4aIII

(Ho et al., 2022, Singh et al., 2019)

elF4AIII

(Haussmann et al., 2022)

l(3)84Fi

(Baker et al., 1991)

Name Synonyms

eIF4AIII

eukaryotic translation initiation factor 4AIII

(Saulière et al., 2010)

lethal(3)84Fi

Secondary FlyBase IDs

FBgn0004221
FBgn0062067

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 48 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40987

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VHS8

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VHS8

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VHS8

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0037573

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40987/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0037573

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0037573

FlyMine - An integrated database for Drosophila genomics

FBgn0037573

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG7483

MARRVEL_MODEL - MARRVEL (model organism gene)

40987

Linkouts

BioGRID - A database of protein and genetic interactions.

66168

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0037573

DroID - A comprehensive database of gene and protein interactions.

FBgn0037573

DRSC - Results frm RNAi screens

FBgn0037573

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

eIF4AIII_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0037573

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0037573

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7483

Flygut - An atlas of the Drosophila adult midgut

FBgn0037573

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0037573

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC009998

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (genetic) - An integrated Molecular Interaction Database

40987

MIST (protein-protein) - An integrated Molecular Interaction Database

40987

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (103)

The following external sites may use different assemblies or annotations than FlyBase.

1.54

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