FlyBase Gene Report: Dmel\gfzf

General Information

Symbol

Dmel\gfzf

Species

D. melanogaster

Name

GST-containing FLYWCH zinc-finger protein

Annotation Symbol

CG33546

Feature Type

protein_coding_gene

FlyBase ID

FBgn0250732

Gene Model Status

Current

Stock Availability

17 publicly available

Enzyme Name (EC)

glutathione transferase (2.5.1.18)

Gene Summary

GST-containing FLYWCH zinc-finger protein (gfzf) encodes encodes a glutathione binding protein that is predicted by phylogeny to have glutathione transferase activity. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Red Book
Interactive Fly

Also Known As

Su(Kpn), lethal (3) 84Cc, dGFZF, l(3)84Cc

Key Links

Genomic Location

Cytogenetic map

84C6-84C6

Sequence location

3R:7,145,880..7,150,968 [-]

Recombination map

3-48

RefSeq locus

NT_033777 REGION:7145880..7150968

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (9 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables DNA-binding transcription factor binding

inferred from physical interaction with FLYBASE:M1BP; FB:FBgn0037621

(Baumann et al., 2018)

enables glutathione binding

inferred from direct assay

(Dai et al., 2004)

enables glutathione transferase activity

inferred from direct assay

(Baumann et al., 2018)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables glutathione transferase activity

inferred from sequence or structural similarity

(Dai et al., 2004)

inferred from biological aspect of ancestor with PANTHER:PTN001679864

(GO Reference Genome Project, 2011-)

enables nucleic acid binding

inferred from sequence or structural similarity

(Dai et al., 2004)

Biological Process (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in positive regulation of transcription by RNA polymerase II

inferred from direct assay

(Baumann et al., 2018)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in glutathione metabolic process

inferred from biological aspect of ancestor with PANTHER:PTN001679864

(GO Reference Genome Project, 2011-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Dai et al., 2004)

colocalizes_with euchromatin

inferred from direct assay

(Baumann et al., 2018)

is_active_in nucleus

inferred from direct assay

(Baumann et al., 2018)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

SOLUBLE GLUTATHIONE S-TRANSFERASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

glutathione transferase activity

RX + glutathione = an S-substituted glutathione + a halide anion + H(+) (2.5.1.18)

RHEA 16437:

Summaries

Gene Snapshot

Gene Group (FlyBase)

SOLUBLE GLUTATHIONE S-TRANSFERASES -: Glutathione transferases (glutathione S-transferases, GST) catalyze nucleophilic attack by reduced glutathione on nonpolar compounds that contain an electrophilic carbon, nitrogen, or sulfur atom. At least 14 classes of soluble GSTs have been identified in prokaryotes and eukaryotes: Theta, Omega, Sigma and Zeta exist in all eukaryotes. D. melanogaster has two additional classes, Delta and Epsilon. In D. mel, the class is indicated by the letter after the Gst prefix. (Adapted from FBrf0217315 and PMID:15822171).

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

l(3)84Cc

Pupal lethal.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\gfzf for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q6NP69)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

gfzf-RB

FBtr0091512

NM_001014610

3910

1045

gfzf-RD

FBtr0114531

NM_001014609

1200

234

gfzf-RE

FBtr0334671

NM_001275406

3608

1045

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

gfzf-PB

FBpp0290855

119.3

1045

5.87

FBpp0113023

27.0

234

5.08

FBpp0306732

119.3

1045

5.87

Q6NP69

NP_001262335

AGB95718

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1045 aa isoforms: gfzf-PB, gfzf-PE

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\gfzf using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.21

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism | intense | ubiquitous

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

amnioserosa

(Fisher et al., 2012)

embryonic brain

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Dai et al., 2004)

colocalizes_with euchromatin

inferred from direct assay

(Baumann et al., 2018)

is_active_in nucleus

inferred from direct assay

(Baumann et al., 2018)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\gfzf in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\gfzf

View exonic expression by tissue for Dmel\gfzf

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0250732

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40858/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0250732

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0250732

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG33546

Flygut - An atlas of the Drosophila adult midgut

FBgn0250732

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 30 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of gfzf

Transgenic constructs containing regulatory region of gfzf

Aberrations (Deficiencies and Duplications) ( 15 )

Inferred from experimentation ( 15 )

Gene disrupted in

Df(3R)Antp17

(Provost et al., 2006)

Df(3R)Antp-A41

(Cavener et al., 1986)

Df(3R)Antp-X1

(Cavener et al., 1986)

Df(3R)dsx2M

(Provost et al., 2006, Cavener et al., 1986)

Df(3R)ED5221

(Ambrus et al., 2009)

Df(3R)ED7665

(Ambrus et al., 2009)

Df(3R)Scx4

(Cavener et al., 1986)

Gene not disrupted in

Df(3R)D6

(Cavener et al., 1986)

Df(3R)dsx10M

(Cavener et al., 1986)

Df(3R)dsx29

(Cavener et al., 1986)

Df(3R)F76

(Cavener et al., 1986)

Df(3R)Scr

(Cavener et al., 1986)

Df(3R)Scx2

(Cavener et al., 1986)

Df(3R)Tpl10

(Cavener et al., 1986)

Df(3R)WIN3

(Cavener et al., 1986)

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

gfzf^GD10231

lethal, with Scer\GAL4^insc-Mz1407

lethal (with Df(3R)Antp7)

gfzf^Su(Kpn)1

lethal (with Df(3R)Antp17)

gfzf^Su(Kpn)1

lethal (with Df(3R)dsx2M)

gfzf^Su(Kpn)1

lethal (with Df(3R)ED5221)

gfzf^6a27

lethal (with Df(3R)ED7665)

gfzf^6a27

lethal (with gfzf^6a27)

gfzf^EY08448

lethal (with gfzf^AP434)

lethal (with gfzf^BU640)

lethal (with gfzf^BW914)

lethal (with gfzf^BX123)

lethal (with gfzf^CK438)

lethal (with gfzf^CX322)

lethal (with gfzf^CZ811)

lethal (with gfzf^EY08448)

gfzf^6a27

lethal (with gfzf^Su(Kpn)1)

lethal (with gfzf^Su(Kpn)2)

lethal (with gfzf^Su(Kpn)3)

lethal | recessive

lethal - all die before end of pupal stage

lethal - all die during embryonic stage

gfzf⁵⁴¹

lethal - all die during embryonic stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

gfzf^GD10231

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

gfzf^GD10231

partially lethal - majority die, with Scer\GAL4^pnr-MD237

viable

gfzf^CL1027

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

gfzf^GD10273

viable (with gfzf^AB621)

viable (with gfzf^AB924)

viable (with gfzf^CL1027)

viable (with gfzf^CU338)

viable (with gfzf^DC727)

viable (with gfzf^DC806)

viable (with gfzf^Su(Kpn)1)

viable (with gfzf^Su(Kpn)2)

viable (with gfzf^Su(Kpn)3)

Other Phenotypes

Allele

abnormal flight, with Scer\GAL4^Mef2.PR

gfzf^GD10273

abnormal neuroanatomy | adult stage, with Scer\GAL4^VGlut1-OK371

gfzf^UAS.cSa

abnormal neuroanatomy | adult stage | somatic clone

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

abnormal neurophysiology | adult stage | somatic clone

gfzf⁵⁴¹

some die during pupal stage

some die during pupal stage, with Scer\GAL4^pnr-MD237

visible, with Scer\GAL4^pnr-MD237

Phenotype manifest in

Allele

axon | adult stage, with Scer\GAL4^VGlut1-OK371

gfzf^UAS.cSa

axon | adult stage | somatic clone

cell body | adult stage | somatic clone

gfzf⁵⁴¹

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

mesonotum, with Scer\GAL4^pnr-MD237

mitochondrion | adult stage, with Scer\GAL4^VGlut1-OK371

gfzf^UAS.cSa

mitochondrion | adult stage | somatic clone

retina | somatic clone

gfzf⁵⁴¹

trichogen cell, with Scer\GAL4^pnr-MD237

gfzf^GD10273

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (27)

Hsap\GSTT2B

5 of 14

Yes

4 of 14

4 of 14

4 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\MARS1

1 of 14

Hsap\ODC1

1 of 14

Hsap\VARS1

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (27)

Rnor\Gstt1

5 of 14

Yes

Rnor\Gstt3

5 of 14

Yes

Rnor\Gstt4

5 of 14

Yes

4 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Mars1

1 of 14

Rnor\Vars1

1 of 14

Mus musculus (laboratory mouse) (20)

Mmus\Gstt1

5 of 14

Yes

Mmus\Gstt2

5 of 14

Yes

Mmus\Gstt3

5 of 14

Yes

Mmus\Gstt4

5 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (13)

Xtro\gstt1-like.2

4 of 13

Yes

3 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (15)

Drer\gstt1a

5 of 14

Yes

Drer\gstt1b

5 of 14

Yes

4 of 14

3 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (54)

Cele\gst-15

3 of 14

Yes

Cele\gst-24

3 of 14

Yes

Cele\gst-27

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (35)

Agam\GSTU1

8 of 12

Yes

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

4 of 12

4 of 12

4 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (57)

Atha\GSTF8

7 of 13

Yes

Atha\GSTF9

7 of 13

Yes

Atha\GSTF10

7 of 13

Yes

Atha\GSTF14

7 of 13

Yes

6 of 13

6 of 13

6 of 13

6 of 13

6 of 13

6 of 13

6 of 13

6 of 13

5 of 13

5 of 13

5 of 13

5 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (6)

Scer\GTT2

4 of 13

Yes

Scer\URE2

4 of 13

Yes

Scer\CAM1

2 of 13

Scer\GTT1

1 of 13

Yes

Scer\TEF4

1 of 13

Scer\YGR201C

1 of 13

Schizosaccharomyces pombe (Fission yeast) (5)

Spom\gst1

4 of 12

Yes

Spom\gst2

4 of 12

Yes

Spom\SPCC1183.02

2 of 12

Spom\gst3

1 of 12

Spom\SPAC29A4.02c

1 of 12

Escherichia coli (enterobacterium) (9)

Ecol\gstB

5 of 11

Yes

4 of 11

4 of 11

4 of 11

2 of 11

Yes

Ecol\sspA

2 of 11

Ecol\yncG

2 of 11

Yes

Ecol\grxB

1 of 11

Yes

Ecol\yibF

1 of 11

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:gfzf. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (46)

8 of 13

8 of 13

8 of 13

8 of 13

8 of 13

8 of 13

8 of 13

8 of 13

8 of 13

8 of 13

8 of 13

8 of 13

8 of 13

7 of 13

7 of 13

7 of 13

7 of 13

7 of 13

7 of 13

7 of 13

7 of 13

7 of 13

7 of 13

7 of 13

7 of 13

5 of 13

5 of 13

5 of 13

5 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

GSTT2B; glutathione S-transferase theta 2B

5 of 14

GSTT1; glutathione S-transferase theta 1

4 of 14

600436

GSTT2; glutathione S-transferase theta 2 (gene/pseudogene)

4 of 14

600437

GSTT4; glutathione S-transferase theta 4

4 of 14

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Dmel gene

Ortholog showing functional complementation

Supporting References

gfzf

Hsap\GSTT1

(Smith et al., 2019)

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

gfzf - Blos1

two hybrid array

(Shokri et al., 2019)

gfzf - CG31441

two hybrid array

(Shokri et al., 2019)

gfzf - Chro

pull down, Identification by mass spectrometry

(Kaushal et al., 2022)

gfzf - Cp190

pull down, Identification by mass spectrometry

(Kaushal et al., 2022)

gfzf - Hr78

two hybrid array

(Shokri et al., 2019)

gfzf - M1BP

pull down, molecular weight estimation by staining

(Baumann et al., 2018)

gfzf - her

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

gfzf

suppressible

Drp1

(Smith et al., 2019)

gfzf

suppressible

GstO1

(Smith et al., 2019)

gfzf

suppressible

GstS1

(Smith et al., 2019)

gfzf

suppressible

Marf

(Smith et al., 2019)

gfzf

suppressible

Opa1

(Smith et al., 2019)

gfzf

suppressible

Pink1

(Smith et al., 2019)

gfzf

suppressible

Prx2

(Smith et al., 2019)

gfzf

suppressible

Trxr1

(Smith et al., 2019)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

E2f1

suppressible

gfzf

(Ambrus et al., 2009)

Marf

suppressible

gfzf

(Smith et al., 2019)

Opa1

suppressible

gfzf

(Smith et al., 2019)

Pink1

suppressible

gfzf

(Smith et al., 2019)

Ras85D

suppressible

gfzf

(Ashton-Beaucage et al., 2014)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

66053

DroID - A comprehensive database of gene and protein interactions.

FBgn0250732

MIST (protein-protein) - An integrated Molecular Interaction Database

40858

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Class of Gene

gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-48

Cytogenetic map

84C6-84C6

Sequence location

3R:7,145,880..7,150,968 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

84C6-84C6

Limits computationally determined from genome sequence between P{lacW}l(3)L2100^L2100 and P{EP}EP3060^EP3060

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-48

(FlyBase, 2016)

3-44.2

(Comeron et al., 2012)

3-[47.5]

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (17)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

67718

w¹¹¹⁸; PBac{gfzf-GFP.FPTB}VK00037

2574

gfzf² p^p cu¹/TM3, Sb¹

32399

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00394}attP2

51102

y¹ w^*; Mi{MIC}gfzf^MI08697 CG2656^MI08697

84063

y¹ sc^* v¹ sev²¹; P{SAM.dCas9.GS05528}attP40

9434

y¹ w^*; gfzf¹/TM3, P{GAL4-Kr.C}DC2, P{UAS-GFP.S65T}DC10, Sb¹

9435

y¹ w^*; gfzf²/TM3, P{GAL4-Kr.C}DC2, P{UAS-GFP.S65T}DC10, Sb¹

Kyoto

107405

gfzf² p^p cu¹/TM3, Sb¹

113684

w^*; P{GawB}gfzf^NP5337 / TM6, P{UAS-lacZ.UW23-1}UW23-1

VDRC

v33931

w¹¹¹⁸; P{GD10273}v33931

v25747

w¹¹¹⁸; P{GD10231}v25747/TM3

v341499

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD00570}attP40/CyO-GFP

More stocks available...

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (57)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

DPiM_RE40740

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0250732

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0250732

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Cooper et al., 2019)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Pupal lethal.

Relationship to Other Genes

Source for database merge of

Source for merge of: gfzf l(3)84Cc

(Provost et al., 2006)

Source for merge of: gfzf CG31492

(FlyBase Genome Annotators, 2005)

Additional comments

Annotations CG31329 and CG31492 merged as CG33546 (which corresponds to gfzf) in release 4.1 of the genome annotation.

(FlyBase Genome Annotators, 2005)

Annotation CG10065 split into CG31492 and CG31329 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Source for identity of gfzf CG31329 was sequence comparison ( date:040330 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: gfzf CG31329

(FlyBase, 1996-)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

CG10065

(Provost et al., 2006, Provost et al., 2005, Dai, 2004.2.9, Dai et al., 2004, Marco et al., 2004, Ranson, 2002.8.27, Ranson et al., 2001)

CG31329

(Ambrus et al., 2009, Dai et al., 2004)

CG31492

(Dai et al., 2004, Giot et al., 2003)

CG33546

(Hosono et al., 2015, Lin et al., 2015, Rosa-Ferreira et al., 2015, Fukuoh et al., 2014, Ni et al., 2010.12.1)

GFZF

(Baumann et al., 2018, Kondo and Perrimon, 2011, Dai, 2004.2.9)

Gfzf

(Li et al., 2024, Kaushal et al., 2022)

Su(Kpn)

(Provost et al., 2006, Provost et al., 2005)

cg10065

(Low et al., 2007)

dGFZF

(Provost et al., 2005, Dai et al., 2004)

gfzf

(Fisher et al., 2023, Patlar and Civetta, 2021, Sigalova et al., 2020, Cooper et al., 2019, Haberle et al., 2019, Shokri et al., 2019, Smith et al., 2019, Baumann et al., 2018, Ashton-Beaucage and Therrien, 2017, Transgenic RNAi Project members, 2017-, Gene Disruption Project members, 2016-, Phadnis et al., 2015, Gonzalez et al., 2014, Friedman et al., 2011, Kallappagoudar et al., 2010, Ambrus et al., 2009, Sims et al., 2009, Li et al., 2008, Provost et al., 2006)

l(3)84Cc

(Provost et al., 2006)

Name Synonyms

GST-containing FLYWCH zinc-finger protein

(Ambrus et al., 2009)

Suppressor of Killer of prune

(Provost et al., 2006)

lethal (3) 84Cc

(Provost et al., 2006)

Secondary FlyBase IDs

FBpp0081126
FBtr0081608
FBpp0081125
FBtr0081607
FBgn0051492
FBgn0051329
FBgn0037479
FBgn0053546
FBgn0002305

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 60 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40858

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q6NP69

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q6NP69

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0250732

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40858/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0250732

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0250732

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG33546

MARRVEL_MODEL - MARRVEL (model organism gene)

40858

Linkouts

BioGRID - A database of protein and genetic interactions.

66053

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0250732

DroID - A comprehensive database of gene and protein interactions.

FBgn0250732

DRSC - Results frm RNAi screens

FBgn0250732

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0250732

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG33546

Flygut - An atlas of the Drosophila adult midgut

FBgn0250732

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC000522

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (protein-protein) - An integrated Molecular Interaction Database

40858

References (74)

The following external sites may use different assemblies or annotations than FlyBase.

0.21

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