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Citation
Alyagor, I., Berkun, V., Keren-Shaul, H., Marmor-Kollet, N., David, E., Mayseless, O., Issman-Zecharya, N., Amit, I., Schuldiner, O. (2018). Combining Developmental and Perturbation-Seq Uncovers Transcriptional Modules Orchestrating Neuronal Remodeling.  Dev. Cell 47(1): 38--52.e6.
FlyBase ID
FBrf0240300
Publication Type
Research paper
Abstract

Developmental neuronal remodeling is an evolutionarily conserved mechanism required for precise wiring of nervous systems. Despite its fundamental role in neurodevelopment and proposed contribution to various neuropsychiatric disorders, the underlying mechanisms are largely unknown. Here, we uncover the fine temporal transcriptional landscape of Drosophila mushroom body γ neurons undergoing stereotypical remodeling. Our data reveal rapid and dramatic changes in the transcriptional landscape during development. Focusing on DNA binding proteins, we identify eleven that are required for remodeling. Furthermore, we sequence developing γ neurons perturbed for three key transcription factors required for pruning. We describe a hierarchical network featuring positive and negative feedback loops. Superimposing the perturbation-seq on the developmental expression atlas highlights a framework of transcriptional modules that together drive remodeling. Overall, this study provides a broad and detailed molecular insight into the complex regulatory dynamics of developmental remodeling and thus offers a pipeline to dissect developmental processes via RNA profiling.

PubMed ID
PubMed Central ID
PMC6179959 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase

Location data for chinmo, Sox14, and mamo deletions.
Alyagor and Schuldiner, 2019.3.17, Location data for chinmo, Sox14, and mamo deletions. [FBrf0241824]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Dev. Cell
    Title
    Developmental Cell
    Publication Year
    2001-
    ISBN/ISSN
    1534-5807 1878-1551
    Data From Reference
    Alleles (98)
    Genes (50)
    Natural transposons (1)
    Insertions (3)
    Transgenic Constructs (88)