FlyBase Gene Report: Dmel\NPF

General Information

Symbol

Dmel\NPF

Species

D. melanogaster

Name

neuropeptide F

Annotation Symbol

CG10342

Feature Type

protein_coding_gene

FlyBase ID

FBgn0027109

Gene Model Status

Current

Stock Availability

14 publicly available

Gene Summary

neuropeptide F (NPF) encodes a 36-residue amidated peptide that signals through its receptor NPFR activating an inhibitory G-protein. Major functions of NPF signaling include the regulation of feeding and courtship behavior, metabolism, alcohol sensitivity, aggression as well as learning and memory. It further plays a role in the regulation of circadian behavior, in particular by modifying evening activity and free-running period. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

dNPF, sNPF, Drm-NPF, Dm-NPF

Key Links

Genomic Location

Cytogenetic map

89D5-89D5

Sequence location

3R:16,609,220..16,611,325 [+]

Recombination map

3-59

RefSeq locus

NT_033777 REGION:16609220..16611325

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (17 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables G protein-coupled receptor binding

inferred from physical interaction with FLYBASE:NPFR; FB:FBgn0037408

(Garczynski et al., 2005)

enables neuropeptide F receptor binding

inferred from physical interaction with FLYBASE:NPFR; FB:FBgn0037408

(Johnson et al., 2003, Garczynski et al., 2002)

enables neuropeptide hormone activity

inferred from mutant phenotype

(Brown et al., 1999)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables neuropeptide hormone activity

traceable author statement

(vanden Broeck, 2001)

non-traceable author statement

(Hewes and Taghert, 2001)

Biological Process (13 terms)

Terms Based on Experimental Evidence (12 terms)

CV Term

Evidence

References

involved_in circadian behavior

inferred from direct assay

(Hermann et al., 2012)

involved_in circadian rhythm

inferred from expression pattern

(Johard et al., 2009)

involved_in G protein-coupled receptor signaling pathway

inferred from direct assay

(Garczynski et al., 2002)

involved_in larval feeding behavior

inferred from mutant phenotype

(Wu et al., 2003)

involved_in larval foraging behavior

inferred from mutant phenotype

(Wu et al., 2003)

inferred from mutant phenotype

(Brown et al., 1999)

involved_in locomotor rhythm

inferred from mutant phenotype

(Lee et al., 2006)

involved_in male courtship behavior

inferred from mutant phenotype

(Lee et al., 2006)

involved_in neuropeptide signaling pathway

inferred from direct assay

(Johnson et al., 2003)

inferred from expression pattern

(Johard et al., 2009)

involved_in olfactory behavior

inferred from mutant phenotype

(Lee et al., 2017)

involved_in regulation of response to food

inferred from mutant phenotype

(Brown et al., 1999)

involved_in response to odorant

inferred from mutant phenotype

(Lee et al., 2017)

involved_in social behavior

inferred from mutant phenotype

(Brown et al., 1999)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in larval feeding behavior

traceable author statement

(Sokolowski, 2003)

involved_in larval locomotory behavior

traceable author statement

(Sokolowski, 2003)

involved_in neuropeptide signaling pathway

traceable author statement

(vanden Broeck, 2001)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in extracellular region

inferred from direct assay

(Brown et al., 1999)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

NEUROPEPTIDES

Protein Family (UniProt)

Belongs to the NPY family. (Q9VET0)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

NEUROPEPTIDES -: Neuropeptides are secreted into the extracellular space where they interact with cell surface receptors (usually G protein coupled receptors). They are extremely diverse, acting as neurotransmitters, neuromodulators, hormones or growth factors. (Adapted from FBrf0211443 and PMID:27813667).

Protein Function (UniProtKB)

Integral part of the sensory system that mediates food signaling, providing the neural basis for the regulation of food response; coordinates larval foraging and social behavior changes during development. Required in dopaminergic (DA) neurons that innervate the mushroom body for satiety to suppress appetitive memory performance; a key factor in the internal state of hunger in the brain. NPF neurons coordinately modulate diverse sensory and motor neurons important for feeding, flight, and locomotion. NPF/NPFR pathway exerts its suppressive effect on larval aversion to diverse stressful stimuli (chemical stress and noxious heat) through attenuation of TRP channel-induced neuronal excitation. NPF neural signaling system plays a physiological role in acute modulation of alcohol sensitivity in adults, rather than a general response to intoxication by sedative agents. Activation and inhibition of the NPF system reduces and enhances ethanol preference, respectively. Sexual experience, the NPF system activity and ethanol consumption are all linked; sexual deprivation is a major contributor to enhanced ethanol preference.

(UniProt, Q9VET0)

Summary (Interactive Fly)

peptide hormone - developmentally programmed to modify foraging and social behavior in Drosophila larvae

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\NPF for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VET0)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.45

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

NPF-RA

FBtr0083328

NM_080493

569

102

NPF-RB

FBtr0331685

NM_001275713

518

102

NPF-RC

FBtr0331686

NM_001275714

579

102

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

NPF-PA

FBpp0082778

11.5

102

7.92

FBpp0304074

11.5

102

7.92

FBpp0304075

11.5

102

7.92

Q9VET0

NP_001262643

AGB96023

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

102 aa isoforms: NPF-PA, NPF-PB, NPF-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\NPF using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

NPF_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.69

Transcript Expression

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult fat body

Comment: slightly cyclical, peak ZT20

(Erion et al., 2016)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

embryonic/larval midgut | restricted

(Veenstra and Ida, 2014)

enteroendocrine cell

(Veenstra and Ida, 2014)

adult stage

LNd neuron | subset

(Hermann et al., 2012)

adult Pdf negative s-LNv neuron

(Hermann et al., 2012)

l-LNv neuron | subset

(Hermann et al., 2012)

fan-shaped body | restricted

(Hermann et al., 2012)

adult brain | central | restricted

(Hermann et al., 2012)

adult brain | lateral | restricted

(Hermann et al., 2012)

adult brain | restricted

(Kahsai and Winther, 2011)

fan-shaped body | dorsal

(Kahsai and Winther, 2011)

fan-shaped body | ventral

(Kahsai and Winther, 2011)

adult subesophageal zone

(Krashes et al., 2009)

protocerebrum | dorsal

(Krashes et al., 2009)

protocerebrum | lateral

(Krashes et al., 2009)

fan-shaped body layer 8

(Krashes et al., 2009)

adult midgut | restricted

(Veenstra and Ida, 2014)

enteroendocrine cell

(Veenstra and Ida, 2014)

tachykinin neuron | restricted

(Shankar et al., 2015)

adult enteroendocrine cell

(Ameku et al., 2018)

adult NPF neuron

(Shao et al., 2017)

Additional Descriptive Data

NPF is expressed in 7 tachykinin neurons in the anterior ventrolateral protocerebrum labelled by ScerGAL4^Tk

(Shankar et al., 2015)

NPF protein labels some of the clock neurons, including, in males, three LNd neurons, three or four l-LNv neurons (weaker signal) and the Pdf negative s-LNV neuron. Females express NPF protein in one less LNd and l-LNv neurons on average than males. Labelling was strongly detected in other non-clock cells in the central and lateral brain, including in the fan-shaped body. The expression pattern is similar to that of ScerGAL4^npf.1, except that varicosities and axon terminals are more strongly stained with NPF protein.

(Hermann et al., 2012)

NPF protein is detected in a dorsal and ventral layers of the fan-shaped body.

(Kahsai and Winther, 2011)

NPF protein is detected in neurons whose cell bodies are in the subesophageal ganglion. These neurons are not labelled by ScerGAL4^npf.1. NPF protein is also detected in the dorsal and lateral protocerebrum, and fan-shaped body.

(Krashes et al., 2009)

Marker for

adult NPF neuron

(Shao et al., 2017)

Subcellular Localization

CV Term

Evidence

References

located_in extracellular region

inferred from direct assay

(Brown et al., 1999)

Expression Deduced from Reporters

Reporter: P{NPF-GAL4.1}

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval NPF-DL1 cell

(Rohwedder et al., 2015)

larval NPF-DL2 cell

(Rohwedder et al., 2015)

larval NPF-SOG cell

(Rohwedder et al., 2015)

early third instar larval stage

peptidergic neuron | subset

(Wu et al., 2003)

larval protocerebrum | restricted

(Wu et al., 2003)

peptidergic neuron of protocerebrum

(Wu et al., 2003)

Reporter: P{NPF-GAL4.4.5}

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval protocerebrum | restricted

(Wu et al., 2003)

embryonic/larval copper cell

(Veenstra, 2009)

embryonic/larval midgut large flat cell

(Veenstra, 2009)

early third instar larval stage

peptidergic neuron | subset

(Wu et al., 2003)

peptidergic neuron of protocerebrum

(Wu et al., 2003)

adult stage

peptidergic neuron

(Nässel et al., 2008)

adult protocerebrum

(Nässel et al., 2008)

adult stage | male

LNd neuron | subset

(Johard et al., 2009)

Reporter: P{NPF-GAL4.U}

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval brain | restricted

(Johard et al., 2008)

adult stage

adult brain | restricted

(Johard et al., 2008)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\NPF in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\NPF

View exonic expression by tissue for Dmel\NPF

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0027109

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42018/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0027109

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0027109

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0027109

Flygut - An atlas of the Drosophila adult midgut

FBgn0027109

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 14 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 13 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of NPF

Transgenic constructs containing regulatory region of NPF

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3R)Exel6270

(Ryder, 2004.4.26)

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

NPF^GD6818

viable, with Scer\GAL4^Mef2.PR

NPF^GD6818

viable, with Scer\GAL4^pnr-MD237

NPF^GD6818

Other Phenotypes

Allele

abnormal behavior | adult stage, with Scer\GAL4^NPF.1

NPF^UAS.cWa

abnormal behavior | larval stage, with Scer\GAL4^386Y

NPF^UAS.cWa

abnormal chemosensory behavior | larval stage, with Scer\GAL4^NPF.1

NPF^RNAi.UAS.cUa

abnormal courtship behavior

abnormal courtship behavior, with Scer\FLP1^fru, Scer\GAL4^NPF.1, Scer\GAL80^αTub84B.PG

NPF^KK111846

abnormal courtship behavior, with Scer\GAL4^elav.PLu

NPF^KK111846

abnormal courtship behavior, with Scer\GAL4^NP0021

NPF^KK111846

abnormal courtship behavior, with Scer\GAL4^NPF.1

NPF^KK111846

abnormal courtship behavior (with NPF¹)

NPF^lexA

abnormal courtship behavior (with NPF^lexA)

NPF¹

abnormal developmental rate | conditional, with Scer\GAL4^386Y

NPF^UAS.cWa

abnormal feeding behavior | larval stage, with Scer\GAL4^386Y

NPF^UAS.cWa

abnormal feeding behavior | larval stage, with Scer\GAL4^NPF.1

NPF^RNAi.UAS.cUa

abnormal mating behavior | mated female, with Scer\GAL4^nSyb.PU

NPF^KK111846

abnormal neurophysiology | adult stage, with Scer\GAL4^386Y

NPF^KK111846

abnormal neurophysiology | adult stage, with Scer\GAL4^Or22a.7.717

NPF^KK111846

abnormal neurophysiology | adult stage, with Scer\GAL4^Orco.PK

NPF^KK111846

abnormal sleep | calorie restriction conditional

NPF^SK2

abnormal starvation stress response | adult stage, with Scer\GAL4^Tk.PS

abnormal starvation stress response | adult stage (with Df(3R)ED10642)

NPF^sk1

chemical sensitive, with Scer\GAL4^386Y

NPF^UAS.cWa

chemical sensitive, with Scer\GAL4^NPF.1

NPF^UAS.cWa

decreased cell number | adult stage, with Scer\GAL4^Tk.PS

NPF^KK111846

decreased cell number | oogenesis | conditional, with Scer\GAL4^GMR41D08

NPF^JF02555

decreased cell number | oogenesis | conditional, with Scer\GAL4^GMR42C03

NPF^JF02555

decreased cell number | oogenesis | conditional, with Scer\GAL4^GMR46G06

NPF^JF02555

decreased cell number | oogenesis | conditional, with Scer\GAL4^Tk.PS

NPF^JF02555

decreased cell number | oogenesis | conditional (with Df(3R)ED10642)

decreased fecundity | female | conditional, with Scer\GAL4^Tk.PS

NPF^JF02555

decreased occurrence of cell division | oogenesis | conditional, with Scer\GAL4^Tk.PS

NPF^JF02555

increased cell number | oogenesis | conditional, with Df(3R)ED10642/NPF^sk1, Scer\GAL4^Tk.PS

NPF^UAS.cWa

increased cell number | oogenesis | conditional, with Scer\GAL4^NP1624

NPF^UAS.cWa

increased cell number | oogenesis | conditional (with Df(3R)ED10642), with NPF^UAS.cWa, Scer\GAL4^Tk.PS

NPF^sk1

increased rate of feeding behavior | adult stage, with Scer\GAL4^Tk.PS

NPF^KK111846

increased rate of feeding behavior | adult stage (with Df(3R)ED10642)

NPF^sk1

long lived, with Scer\GAL4^NPF.1

NPF^JF02555

long lived | nutrition conditional, with Scer\GAL4^Tk.PS

NPF^JF02555

Phenotype manifest in

Allele

adult enteroendocrine cell | decreased number, with Scer\GAL4^Tk.PS

NPF^KK111846

adult olfactory receptor neuron Or22, with Scer\GAL4^386Y

NPF^KK111846

adult olfactory receptor neuron Or22, with Scer\GAL4^Or22a.7.717

NPF^KK111846

adult olfactory receptor neuron Or22, with Scer\GAL4^Orco.PK

NPF^KK111846

female germline stem cell | conditional, with Df(3R)ED10642/NPF^sk1, Scer\GAL4^Tk.PS

NPF^UAS.cWa

female germline stem cell | conditional, with Scer\GAL4^GMR41D08

NPF^JF02555

female germline stem cell | conditional, with Scer\GAL4^GMR42C03

NPF^JF02555

female germline stem cell | conditional, with Scer\GAL4^GMR46G06

NPF^JF02555

female germline stem cell | conditional, with Scer\GAL4^NP1624

NPF^UAS.cWa

female germline stem cell | conditional, with Scer\GAL4^Tk.PS

NPF^JF02555

female germline stem cell | conditional (with Df(3R)ED10642)

female germline stem cell | conditional (with Df(3R)ED10642), with NPF^UAS.cWa, Scer\GAL4^Tk.PS

NPF^sk1

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (0)

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (0)

Mus musculus (laboratory mouse) (0)

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (0)

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (1)

Agam\NPF_ANOGA

1 of 12

Yes

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:NPF. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

NPF^UAS.cWa

model of alcohol use disorder

CEA

(Wen et al., 2005)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

NPF - NPFR

phenotype-based detection assay, fluorescence microscopy, inferred by author

(Johnson et al., 2003)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

NPF

suppressible

AkhR

(Yoshinari et al., 2021)

NPF

suppressible

Hsl

(Yoshinari et al., 2021)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

TrpA1

suppressible

NPF

(Chung et al., 2017)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0027109

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-59

Cytogenetic map

89D5-89D5

Sequence location

3R:16,609,220..16,611,325 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

89D5-89D5

Limits computationally determined from genome sequence between P{lacW}CSN5^L4032 and P{EP}MESK4^EP1015

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-59

(FlyBase, 2016)

3-58.7

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (14)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

83722

w¹¹¹⁸; NPF¹

84549

w^*; TI{TI}NPF^attP

39254

w¹¹¹⁸; P{GMR60F04-GAL4}attP2

39431

w¹¹¹⁸; P{GMR67D02-GAL4}attP2

25681

y¹ w^*; P{NPF-GAL4.1}2

25682

y¹ w^*; P{NPF-GAL4.1}1

27237

y¹ v¹; P{TRiP.JF02555}attP2

84671

w^*; TI{2A-GAL4}NPF^2A-GAL4/TM6B, Tb¹

83720

w¹¹¹⁸; TI{lexA::p65}NPF^lexA/TM6B, Tb¹

84422

w^*; TI{2A-lexA::GAD}NPF^2A-lexA/TM3, Sb¹

VDRC

v330277

P{VSH330277}attP40

v108772

P{KK111846}VIE-260B

More stocks available...

Genomic Clones (22)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (5)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH04563

Other clones

DPiM_GH04563

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0027109

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0027109

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Lee et al., 2025, Yoshinari et al., 2021, Veenstra and Ida, 2014, Hermann et al., 2012)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Larvae deficient in npf signaling precociously show the phenotypes of food aversion and social behaviours normally shown by older nonfeeding larvae.

(Wu et al., 2003)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: npf NPF

(FlyBase, 2009-)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (21)

Reported As

Symbol Synonym

CG10342

(de Tredern et al., 2025, Mao et al., 2024, Meadows, 2017.1.11, Lemaitre and Miguel-Aliaga, 2013, Ida et al., 2011, Reiher et al., 2011, Keleman et al., 2009.8.5, Yew et al., 2009, Wegener and Gorbashov, 2008, De Loof et al., 2001)

Dm-NPF

(Collin et al., 2011, Lee et al., 2004)

DmNPF

(Garczynski et al., 2002)

Drm-NPF

(Garczynski et al., 2005, Nassel, 2002, vanden Broeck, 2001)

NP-PP

(Hewes and Taghert, 2001)

NPF

(Ahmad et al., 2025, Asahina and Zelikowsky, 2025, Chen et al., 2025, de Tredern et al., 2025, Lee et al., 2025, Lee et al., 2025, Lee et al., 2025, Liu et al., 2025, Nässel, 2025, Nässel, 2025, Shi et al., 2025, Tawa and Notterman, 2025, Xia and Li, 2025, Yapici, 2025, Bhandari et al., 2024, Dayal Aggarwal et al., 2024, Lin et al., 2024, Mahishi et al., 2024, Peterson and Ahmad, 2024, Ryvkin et al., 2024, Suárez-Grimalt et al., 2024, Chen et al., 2023, Hu et al., 2023, Chopra et al., 2022, Deshpande et al., 2022, Ding et al., 2022, Dvořáček et al., 2022, Honda, 2022, Kubrak et al., 2022, Nässel and Wu, 2022, Palavicino-Maggio and Sengupta, 2022, Pang et al., 2022, White and Wolfner, 2022, Yu et al., 2022, Zhou et al., 2022, Ahmad et al., 2021, Barber et al., 2021, Devineni and Scaplen, 2021, Feng et al., 2021, Hime et al., 2021, Hoshino and Niwa, 2021, Hung et al., 2021, Kannangara et al., 2021, Khan et al., 2021, Koyama et al., 2021, Landayan et al., 2021, Lee et al., 2021, Ma et al., 2021, Mishra et al., 2021, Nässel, 2021, Pang et al., 2021, Sadanandappa et al., 2021, Sareen et al., 2021, Schoberleitner et al., 2021, Shafer and Keene, 2021, Yeom et al., 2021, Yoshinari et al., 2021, Araki et al., 2020, Gao et al., 2020, Hung et al., 2020, Kannangara et al., 2020, Koyama et al., 2020, Lathen et al., 2020, Lee and Wu, 2020, Nässel and Zandawala, 2020, Toprak et al., 2020, Williams et al., 2020, Zhou et al., 2020, Bozler et al., 2019, Deng et al., 2019, Engel et al., 2019, Gu et al., 2019, Hegazi et al., 2019, Kozlov and Nagoshi, 2019, Li et al., 2019, Lin et al., 2019, Rao and Deng, 2019.10.23, Zhang et al., 2019, Ameku et al., 2018, Diesner et al., 2018, Gáliková and Klepsatel, 2018, Grabowska et al., 2018, Helfrich-Förster, 2018, Lowenstein and Velazquez-Ulloa, 2018, Nässel, 2018, Obniski et al., 2018, Pu et al., 2018, Ryvkin et al., 2018, Sayin et al., 2018, Top and Young, 2018, Asahina, 2017, Kim et al., 2017, Kim et al., 2017, Lee et al., 2017, Liang et al., 2017, Loh et al., 2017, Park et al., 2017, Schoofs et al., 2017, Tomita et al., 2017, Transgenic RNAi Project members, 2017-, Chen et al., 2016, Slade and Staveley, 2016, Beckwith and Ceriani, 2015, Grotewiel and Bettinger, 2015, Landayan and Wolf, 2015, Shankar et al., 2015, Amcheslavsky et al., 2014, Song et al., 2014, Veenstra and Ida, 2014, Erion and Sehgal, 2013, Itskov and Ribeiro, 2013, Nässel et al., 2013, Perry and Barron, 2013, Rajan and Perrimon, 2013, Shang et al., 2013, Wang et al., 2013, Bendena et al., 2012, Chintapalli et al., 2012, Hermann et al., 2012, Herrero, 2012, Kaun et al., 2012, Marella et al., 2012, Morozova et al., 2012, Shohat-Ophir et al., 2012, Zwarts et al., 2012, Kahsai and Winther, 2011, Nässel and Wegener, 2011, Nässel and Winther, 2010, Xu et al., 2010, Johard et al., 2009, Rieger et al., 2009, Veenstra, 2009, Park et al., 2008, Veenstra et al., 2008, Xu et al., 2008, Yoshii et al., 2008, Johnson et al., 2005, Wu et al., 2005, Wu et al., 2005, Johnson et al., 2003)

NPF-A1

(Feng et al., 2003)

NPF-A2

(Feng et al., 2003)

NPF/NPY

(Miller et al., 2020)

NPF89D3

(Feng et al., 2003)

Npf

(Lee et al., 2015, Marianes and Spradling, 2013, Kadener et al., 2006)

dNPF

(Dvořáček and Kodrík, 2021, Grabowska et al., 2020, Cognigni et al., 2018, Croset et al., 2018, Perisse et al., 2013, Ida et al., 2011, Newquist, 2011, Nässel and Winther, 2010, Krashes et al., 2009, Yew et al., 2009, Shen et al., 2003, Sokolowski, 2003, Wu et al., 2003, Shen and Cai, 2001)

dnpf

(Tonoki et al., 2020, Shen and Cai, 2001)

sNPF

(Honda, 2022, Nässel and Zandawala, 2022, Megha and Hasan, 2020)

Name Synonyms

NPY-like neuropeptide F

(Chen et al., 2008)

Neuropeptide F

(Boumard and Bardin, 2021, Ahmad et al., 2020, Sachse and Beshel, 2016, Slade and Staveley, 2016, Beckwith and Ceriani, 2015, Asahina et al., 2014, Owusu-Ansah and Perrimon, 2014, Itskov and Ribeiro, 2013, Perisse et al., 2013, Bendena et al., 2012, Caers et al., 2012, Grewal, 2012, Nässel and Winther, 2010, Xu et al., 2010, Nassel, 2002)

Neuropeptide F 1

(Nässel and Winther, 2010)

long neuropeptide F

(Johard et al., 2009)

neuropeptide-F

(Veenstra et al., 2008)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 32 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

42018

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VET0

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VET0

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

A0A0B4KG37

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VET0

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0027109

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42018/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0027109

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0027109

FlyMine - An integrated database for Drosophila genomics

FBgn0027109

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG10342

MARRVEL_MODEL - MARRVEL (model organism gene)

42018

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0027109

DroID - A comprehensive database of gene and protein interactions.

FBgn0027109

DRSC - Results frm RNAi screens

FBgn0027109

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

NPF_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0027109

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0027109

Flygut - An atlas of the Drosophila adult midgut

FBgn0027109

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

References (279)

The following external sites may use different assemblies or annotations than FlyBase.

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