FlyBase Gene Report: Dmel\scaf

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Gene: Dmel\scaf

General Information

Symbol

Dmel\scaf

Species

D. melanogaster

Name

scarface

Annotation Symbol

CG11066

Feature Type

protein_coding_gene

FlyBase ID

FBgn0033033

Gene Model Status

Stock Availability

11 publicly available

Gene Summary

Inactive serine protease that plays a role in germ-band retraction and dorsal closure morphogenesis in embryogenesis; contributes to amnioserosa attachment and epithelial apico-basal polarity by regulating the localization of laminin LanA on the apical side of the amnioserosa epithelium (PubMed:20379222, PubMed:28628612). Contributes to epithelial morphogenesis probably by regulating the bsk/JNK pathway, as part of a negative-feedback loop, and by modulating the cross-talk between the Egfr, bsk/JNK and dpp signal transduction pathways (PubMed:20379222, PubMed:28628612). In larval development, antagonizes the morphogenetic movements controlled by the bsk/JNK signaling including male genitalia formation and thorax development (PubMed:20379222, PubMed:20530545, PubMed:25737837). (UniProt, Q7K5M0)

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All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

Sequence location

2R:5,676,750..5,691,723 [-]

Recombination map

2-55

RefSeq locus

NT_033778 REGION:5676750..5691723

Sequence

Get Decorated FASTA

Genomic Maps

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (19 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

NOT enables peptidase activity

inferred from key residues with MEROPS:S01.UNA

(GO curators, 2012-)

NOT enables serine-type endopeptidase activity

inferred from key residues

(Cao and Jiang, 2018, Ross et al., 2003)

Biological Process (10 terms)

Terms Based on Experimental Evidence (8 terms)

CV Term

Evidence

References

involved_in dorsal closure, elongation of leading edge cells

inferred from mutant phenotype

(Sorrosal et al., 2010)

involved_in dorsal closure

inferred from mutant phenotype

(Rousset et al., 2010, Sorrosal et al., 2010)

involved_in establishment or maintenance of epithelial cell apical/basal polarity

inferred from mutant phenotype

(Sorrosal et al., 2010)

involved_in germ-band shortening

inferred from mutant phenotype

(Sorrosal et al., 2010)

involved_in imaginal disc morphogenesis

inferred from mutant phenotype

(Srivastava and Dong, 2015)

involved_in imaginal disc-derived male genitalia morphogenesis

inferred from mutant phenotype

(Rousset et al., 2010)

involved_in notum morphogenesis

inferred from mutant phenotype

(Srivastava and Dong, 2015)

involved_in regulation of developmental process

inferred from mutant phenotype

(Srivastava and Dong, 2015)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in innate immune response

inferred from biological aspect of ancestor with PANTHER:PTN000667087

(GO Reference Genome Project, 2011-)

involved_in proteolysis

inferred from electronic annotation with InterPro:IPR001254

(InterPro Project Members, 2004-)

Cellular Component (7 terms)

Terms Based on Experimental Evidence (7 terms)

CV Term

Evidence

References

colocalizes_with apical plasma membrane

inferred from direct assay

(Sorrosal et al., 2010)

located_in cytoplasm

inferred from direct assay

(Srivastava and Dong, 2015)

colocalizes_with early endosome

inferred from direct assay

(Sorrosal et al., 2010)

located_in extracellular region

inferred from direct assay

(Sorrosal et al., 2010)

located_in extracellular space

inferred from direct assay

(Rousset et al., 2010)

colocalizes_with late endosome

inferred from direct assay

(Sorrosal et al., 2010)

colocalizes_with recycling endosome

inferred from direct assay

(Sorrosal et al., 2010)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in extracellular space

inferred from biological aspect of ancestor with PANTHER:PTN000667087

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

S1A NON-PEPTIDASE HOMOLOGS

Protein Family (UniProt)

Belongs to the peptidase S1 family. (Q7K5M0)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

S1A NON-PEPTIDASE HOMOLOGS -: Serine protease homologs of the S1A family are similar in amino acid sequence to S1A serine proteases but are enzymatically inactive due to the lack of catalytic residue(s). Their functions are generally poorly understood, though at least some of them have known regulatory functions. (Adapted from FBrf0155784 & FBrf0240739.)

Protein Function (UniProtKB)

Inactive serine protease that plays a role in germ-band retraction and dorsal closure morphogenesis in embryogenesis; contributes to amnioserosa attachment and epithelial apico-basal polarity by regulating the localization of laminin LanA on the apical side of the amnioserosa epithelium (PubMed:20379222, PubMed:28628612). Contributes to epithelial morphogenesis probably by regulating the bsk/JNK pathway, as part of a negative-feedback loop, and by modulating the cross-talk between the Egfr, bsk/JNK and dpp signal transduction pathways (PubMed:20379222, PubMed:28628612). In larval development, antagonizes the morphogenetic movements controlled by the bsk/JNK signaling including male genitalia formation and thorax development (PubMed:20379222, PubMed:20530545, PubMed:25737837).

(UniProt, Q7K5M0)

Summary (Interactive Fly)

belongs to a subgroup of serine protease homologues with an inactive catalytic site - functions extracellularly during dorsal closure as an antagonist of the JNK pathway - regulates the polarized localization of laminin A during the development of epithelial polarity

Interactive Fly

Gene Model and Products

Number of Transcripts

2

Number of Unique Polypeptides

1

Please see the JBrowse view of Dmel\scaf for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q7K5M0)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0086061

3390

655

FBtr0086060

3043

655

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

FBpp0085397

71.4

655

7.05

FBpp0085396

71.4

655

7.05

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

655 aa isoforms: scaf-PA, scaf-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Domain

The CLIP domain consists of 37-55 residues which are 'knitted' together usually by 3 conserved disulfide bonds forming a clip-like compact structure.

(UniProt, Q7K5M0)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Q7K5M0

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\scaf using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.77

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11 -- 12

dorsal epidermis primordium

(Fisher et al., 2012)

embryonic stage 12 -- 14

(Rousset et al., 2010)

ventral ectoderm

(Rousset et al., 2010)

embryonic stage 12 -- 16

embryonic leading edge cell

(Rousset et al., 2010)

embryonic stage 13 -- 16

embryonic dorsal epidermis

(Fisher et al., 2012)

embryonic head epidermis

(Fisher et al., 2012)

embryonic leading edge cell

(Fisher et al., 2012)

embryonic ventral epidermis

(Fisher et al., 2012)

larval pericardial cell

(Fisher et al., 2012)

Additional Descriptive Data

scaf is strongly expressed in the leading edge during dorsal closure. It is also detected in some cells of the amnioserosa and in the ventral ectoderm.

(Rousset et al., 2010)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 12 -- 16

embryonic leading edge cell

(Rousset et al., 2010)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

colocalizes_with apical plasma membrane

inferred from direct assay

(Sorrosal et al., 2010)

located_in cytoplasm

inferred from direct assay

(Srivastava and Dong, 2015)

colocalizes_with early endosome

inferred from direct assay

(Sorrosal et al., 2010)

located_in extracellular region

inferred from direct assay

(Sorrosal et al., 2010)

located_in extracellular space

inferred from direct assay

(Rousset et al., 2010)

colocalizes_with late endosome

inferred from direct assay

(Sorrosal et al., 2010)

colocalizes_with recycling endosome

inferred from direct assay

(Sorrosal et al., 2010)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\scaf in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\scaf

View exonic expression by tissue for Dmel\scaf

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0033033

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35505/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0033033

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0033033

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0033033

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

Flygut - An atlas of the Drosophila adult midgut

FBgn0033033

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\scaf

Stages(s) 11-12
Stages(s) 13-16

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 10 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of scaf

Transgenic constructs containing regulatory region of scaf

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

(Rousset et al., 2010)

Inferred from location ( 4 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | recessive

scaf²⁷

lethal - all die before end of pupal stage | recessive

scaf^PBss

partially lethal - majority die | recessive

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

scaf^GD5263

viable, with Scer\GAL4^Mef2.PR

scaf^GD5263

viable, with Scer\GAL4^pnr-MD237

scaf^GD5263

Other Phenotypes

Allele

abnormal feeding behavior | adult stage | nutrition conditional, with Scer\GAL4^GMR50H04

scaf^UAS.cRa

abnormal feeding behavior | adult stage | nutrition conditional, with Scer\GAL4^nSyb.PS

scaf^HMC03909

some die during pupal stage | recessive

scaf^PBss

visible | recessive

Phenotype manifest in

Allele

embryonic/first instar larval cuticle

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (61)

1 of 14

Yes

No

1 of 14

Yes

No

1

1 of 14

Yes

No

1

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1

1 of 14

Yes

No

1

1 of 14

Yes

Yes

1 of 14

Yes

No

1 of 14

Yes

No

1

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1

1 of 14

Yes

No

1

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (42)

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

Rnor\LOC100361261

1 of 14

Yes

No

Rnor\LOC102553861

1 of 14

Yes

No

1 of 14

Yes

Yes

1 of 14

Yes

Yes

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

Yes

1 of 14

Yes

Yes

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

Mus musculus (laboratory mouse) (43)

Mmus\2210010C04Rik

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

Yes

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

Yes

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

Xenopus tropicalis (Western clawed frog) (39)

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

Yes

1 of 13

Yes

No

Xtro\LOC100495541

1 of 13

Yes

No

Xtro\LOC101731336

1 of 13

Yes

No

Xtro\LOC101732176

1 of 13

Yes

No

Xtro\LOC101733280

1 of 13

Yes

No

Xtro\LOC101734670

1 of 13

Yes

No

Xtro\LOC108648818

1 of 13

Yes

No

Xtro\LOC116407662

1 of 13

Yes

No

Xtro\LOC116408674

1 of 13

Yes

Yes

Xtro\LOC116411715

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

Xtro\tmprss2.12

1 of 13

Yes

No

Xtro\tmprss2.15

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

Danio rerio (Zebrafish) (45)

1 of 14

Yes

Yes

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

Drer\LOC103908930

1 of 14

Yes

No

Drer\LOC110439063

1 of 14

Yes

Yes

Drer\LOC110439065

1 of 14

Yes

Yes

Drer\LOC110439069

1 of 14

Yes

Yes

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

Drer\si:dkey-9p24.5

1 of 14

Yes

Yes

Drer\si:dkey-32n7.7

1 of 14

Yes

No

Drer\si:dkey-78l4.6

1 of 14

Yes

No

Drer\si:dkeyp-93a5.2

1 of 14

Yes

No

Drer\si:dkeyp-93a5.3

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

Drer\zgc:100868

1 of 14

Yes

No

Drer\zgc:112038

1 of 14

Yes

No

Drer\zgc:123217

1 of 14

Yes

No

Drer\zgc:123295

1 of 14

Yes

No

Drer\zgc:153968

1 of 14

Yes

No

Drer\zgc:165423

1 of 14

Yes

No

Drer\zgc:171509

1 of 14

Yes

No

Drer\zgc:171592

1 of 14

Yes

No

Drer\zmp:0000001114

1 of 14

Yes

No

Caenorhabditis elegans (Nematode, roundworm) (3)

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

Anopheles gambiae (African malaria mosquito) (190)

9 of 12

Yes

Yes

Agam\AgaP_AGAP005065

2 of 12

No

No

Agam\AgaP_AGAP005194

2 of 12

No

No

Agam\AgaP_AGAP006707

2 of 12

No

No

Agam\AgaP_AGAP006710

2 of 12

No

No

Agam\AgaP_AGAP007142

2 of 12

No

No

Agam\AgaP_AGAP011917

2 of 12

No

No

Agam\AgaP_AGAP011919

2 of 12

No

No

Agam\CTR2_ANOGA

2 of 12

No

No

Agam\AgaP_AGAP000290

1 of 12

No

No

Agam\AgaP_AGAP000411

1 of 12

No

No

Agam\AgaP_AGAP001199

1 of 12

No

No

Agam\AgaP_AGAP001252

1 of 12

No

No

Agam\AgaP_AGAP001365

1 of 12

No

No

Agam\AgaP_AGAP001964

1 of 12

No

No

Agam\AgaP_AGAP002842

1 of 12

No

No

Agam\AgaP_AGAP003248

1 of 12

No

No

Agam\AgaP_AGAP003627

1 of 12

No

No

Agam\AgaP_AGAP003686

1 of 12

No

No

Agam\AgaP_AGAP003691

1 of 12

No

No

Agam\AgaP_AGAP003748

1 of 12

No

No

Agam\AgaP_AGAP003807

1 of 12

No

No

Agam\AgaP_AGAP003960

1 of 12

No

No

Agam\AgaP_AGAP003971

1 of 12

No

No

Agam\AgaP_AGAP003977

1 of 12

No

Yes

Agam\AgaP_AGAP004149

1 of 12

No

No

Agam\AgaP_AGAP004566

1 of 12

No

No

Agam\AgaP_AGAP004567

1 of 12

No

No

Agam\AgaP_AGAP004568

1 of 12

No

No

Agam\AgaP_AGAP004569

1 of 12

No

No

Agam\AgaP_AGAP004570

1 of 12

No

No

Agam\AgaP_AGAP004571

1 of 12

No

No

Agam\AgaP_AGAP004857

1 of 12

No

Yes

Agam\AgaP_AGAP004858

1 of 12

No

No

Agam\AgaP_AGAP004900

1 of 12

No

No

Agam\AgaP_AGAP005072

1 of 12

No

No

Agam\AgaP_AGAP005195

1 of 12

No

No

Agam\AgaP_AGAP005196

1 of 12

No

No

Agam\AgaP_AGAP005303

1 of 12

No

No

Agam\AgaP_AGAP005310

1 of 12

No

No

Agam\AgaP_AGAP005663

1 of 12

No

No

Agam\AgaP_AGAP005687

1 of 12

No

No

Agam\AgaP_AGAP005704

1 of 12

No

No

Agam\AgaP_AGAP005791

1 of 12

No

No

Agam\AgaP_AGAP006087

1 of 12

No

No

Agam\AgaP_AGAP006192

1 of 12

No

No

Agam\AgaP_AGAP006385

1 of 12

No

No

Agam\AgaP_AGAP006485

1 of 12

No

No

Agam\AgaP_AGAP006486

1 of 12

No

No

Agam\AgaP_AGAP006487

1 of 12

No

No

Agam\AgaP_AGAP006488

1 of 12

No

No

Agam\AgaP_AGAP006489

1 of 12

No

No

Agam\AgaP_AGAP006539

1 of 12

No

No

Agam\AgaP_AGAP006676

1 of 12

No

No

Agam\AgaP_AGAP006677

1 of 12

No

No

Agam\AgaP_AGAP007043

1 of 12

No

No

Agam\AgaP_AGAP007141

1 of 12

No

No

Agam\AgaP_AGAP007251

1 of 12

No

No

Agam\AgaP_AGAP007280

1 of 12

No

No

Agam\AgaP_AGAP007795

1 of 12

No

No

Agam\AgaP_AGAP008276

1 of 12

No

No

Agam\AgaP_AGAP008277

1 of 12

No

No

Agam\AgaP_AGAP008403

1 of 12

No

No

Agam\AgaP_AGAP008558

1 of 12

No

No

Agam\AgaP_AGAP008649

1 of 12

No

No

Agam\AgaP_AGAP008808

1 of 12

No

No

Agam\AgaP_AGAP008861

1 of 12

No

No

Agam\AgaP_AGAP008891

1 of 12

No

No

Agam\AgaP_AGAP008911

1 of 12

No

No

Agam\AgaP_AGAP008994

1 of 12

No

No

Agam\AgaP_AGAP008995

1 of 12

No

No

Agam\AgaP_AGAP008996

1 of 12

No

No

Agam\AgaP_AGAP008997

1 of 12

No

No

Agam\AgaP_AGAP008999

1 of 12

No

Yes

Agam\AgaP_AGAP009006

1 of 12

No

No

Agam\AgaP_AGAP009211

1 of 12

No

No

Agam\AgaP_AGAP009216

1 of 12

No

No

Agam\AgaP_AGAP009218

1 of 12

No

No

Agam\AgaP_AGAP009220

1 of 12

No

No

Agam\AgaP_AGAP009249

1 of 12

No

No

Agam\AgaP_AGAP009251

1 of 12

No

No

Agam\AgaP_AGAP009252

1 of 12

No

No

Agam\AgaP_AGAP009273

1 of 12

No

No

Agam\AgaP_AGAP009828

1 of 12

No

No

Agam\AgaP_AGAP009849

1 of 12

No

No

Agam\AgaP_AGAP009966

1 of 12

No

No

Agam\AgaP_AGAP010528

1 of 12

No

Yes

Agam\AgaP_AGAP010529

1 of 12

No

Yes

Agam\AgaP_AGAP010545

1 of 12

No

No

Agam\AgaP_AGAP010546

1 of 12

No

No

Agam\AgaP_AGAP010627

1 of 12

No

Yes

Agam\AgaP_AGAP010635

1 of 12

No

No

Agam\AgaP_AGAP010662

1 of 12

No

No

Agam\AgaP_AGAP010692

1 of 12

No

No

Agam\AgaP_AGAP010730

1 of 12

No

No

Agam\AgaP_AGAP010798

1 of 12

No

No

Agam\AgaP_AGAP011040

1 of 12

No

No

Agam\AgaP_AGAP011325

1 of 12

No

No

Agam\AgaP_AGAP011427

1 of 12

No

No

Agam\AgaP_AGAP011429

1 of 12

No

Yes

Agam\AgaP_AGAP011477

1 of 12

No

No

Agam\AgaP_AGAP011590

1 of 12

No

No

Agam\AgaP_AGAP011608

1 of 12

No

No

Agam\AgaP_AGAP011669

1 of 12

No

No

Agam\AgaP_AGAP011719

1 of 12

No

No

Agam\AgaP_AGAP011793

1 of 12

No

No

Agam\AgaP_AGAP011794

1 of 12

No

No

Agam\AgaP_AGAP011908

1 of 12

No

No

Agam\AgaP_AGAP011918

1 of 12

No

No

Agam\AgaP_AGAP012020

1 of 12

No

No

Agam\AgaP_AGAP012021

1 of 12

No

No

Agam\AgaP_AGAP012022

1 of 12

No

No

Agam\AgaP_AGAP012269

1 of 12

No

No

Agam\AgaP_AGAP012270

1 of 12

No

No

Agam\AgaP_AGAP012276

1 of 12

No

No

Agam\AgaP_AGAP012277

1 of 12

No

No

Agam\AgaP_AGAP012328

1 of 12

No

No

Agam\AgaP_AGAP012502

1 of 12

No

No

Agam\AgaP_AGAP012504

1 of 12

No

No

Agam\AgaP_AGAP012505

1 of 12

No

Yes

Agam\AgaP_AGAP012614

1 of 12

No

No

Agam\AgaP_AGAP012671

1 of 12

No

No

Agam\AgaP_AGAP012946

1 of 12

No

No

Agam\AgaP_AGAP013089

1 of 12

No

No

Agam\AgaP_AGAP013117

1 of 12

No

Yes

Agam\AgaP_AGAP013221

1 of 12

No

No

Agam\AgaP_AGAP013252

1 of 12

No

No

Agam\AgaP_AGAP013487

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

Yes

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

Yes

1 of 12

No

No

1 of 12

No

No

Agam\CTR1_ANOGA

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

Agam\SP24D_ANOGA

1 of 12

No

No

Agam\TRY1_ANOGA

1 of 12

No

No

Agam\TRY2_ANOGA

1 of 12

No

No

Agam\TRY3_ANOGA

1 of 12

No

No

Agam\TRY4_ANOGA

1 of 12

No

No

Agam\TRY5_ANOGA

1 of 12

No

No

Agam\TRY6_ANOGA

1 of 12

No

No

Agam\TRY7_ANOGA

1 of 12

No

No

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:scaf. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (196)

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Rousset et al., 2010)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0033033

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

2R

Recombination map

2-55

Cytogenetic map

Sequence location

2R:5,676,750..5,691,723 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

41F6-41F8

Limits computationally determined from genome sequence between P{PZ}vlc⁰⁷⁰²²&P{lacW}l(2)09851^k08138 and P{PZ}l(2)09851⁰⁹⁸⁵¹&P{lacW}Src42A^k10108

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-55

(FlyBase, 2016)

2-55.7

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (11)

Aberrations including deletions of this gene Aberrations including duplications of this gene

29386

y¹ v¹; P{TRiP.JF03318}attP2

36974

y¹ w^*; Mi{MIC}scaf^MI03824/SM6a

53101

y¹ w^*; Mi{MIC}scaf^MI09409/SM6a

56349

y¹ w^*; Mi{MIC}scaf^MI11586/SM6a

13631

y¹ w^67c23; P{SUPor-P}scaf^KG05129

55695

y¹ sc^* v¹ sev²¹; P{TRiP.HMC03909}attP40

11066R-1

11066R-2

HMJ22062

y¹ v¹; P{TRiP.HMJ22062}attP40/CyO

v330285

P{VSH330285}attP40

v13249

w¹¹¹⁸; P{GD5263}v13249

Genomic Clones (29)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (97)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH05918

Other clones

DPiM_GH05918

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0033033

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0033033

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Contreras et al., 2021, Rousset et al., 2010, Sorrosal et al., 2010)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Source for merge of: CG11066 CG15900

(Whitfield, 2000.12.15)

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: scarface CG11066

(Bonin and Mann, 2004)

Source for identity of: scaf scarface

(Rousset et al., 2010)

Nomenclature comments

Etymology

The gene is named "scarface" based on the phenotype of homozygous adult escapers, which have what appears to be "scarring" around the mouthparts.

(Bonin and Mann, 2004)

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

CG11066

(Meadows, 2017.1.11, Caizzi et al., 2016, Veillard et al., 2016, Ni et al., 2010.12.1, Perkins et al., 2009, Collett, 2008.2.16, Shah et al., 2008, Kim et al., 2005, Bonin and Mann, 2004, Myster et al., 2004, Corradini et al., 2003)

CG15900

(Boutros et al., 2002)

SPH142

(Ross et al., 2003)

Scaf

(Cao and Jiang, 2018, Veillard et al., 2016, Ríos-Barrera and Riesgo-Escovar, 2013)

cSPH142

(Cao and Jiang, 2018)

scaf

(Brutscher et al., 2025, Rommelaere et al., 2025, Joy et al., 2021, Zhou et al., 2021, Funikov et al., 2020, Parra and Johnston, 2020, Mortensen et al., 2018, Revaitis et al., 2017, Kuleesha et al., 2016, Dent et al., 2015, Doggett et al., 2015, DeSalvo et al., 2014, Lai et al., 2014, Kwon et al., 2013, Lloret-Llinares et al., 2012, Rousset et al., 2010)

scarf

(Sorrosal et al., 2010)

scarface

(Ashwal-Fluss et al., 2014, Japanese National Institute of Genetics, 2012.5.21)

Name Synonyms

Scarface

(Veillard et al., 2016, Sorrosal et al., 2010)

scarface

(Álvarez-Fernández et al., 2015, Lai et al., 2014, Chen et al., 2011, Napoletano et al., 2011, Rousset et al., 2010, Collett, 2008.2.16, Gilchrist et al., 2008, Yakoby et al., 2008, Bonin and Mann, 2004)

Secondary FlyBase IDs

FBgn0033034

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 41 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35505

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q7K5M0

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q7K5M0

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q7K5M0

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0033033

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35505/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0033033

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0033033

FlyMine - An integrated database for Drosophila genomics

FBgn0033033

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG11066

MARRVEL_MODEL - MARRVEL (model organism gene)

35505

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0033033

DroID - A comprehensive database of gene and protein interactions.

FBgn0033033

DRSC - Results frm RNAi screens

FBgn0033033

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0033033

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0033033

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

Flygut - An atlas of the Drosophila adult midgut

FBgn0033033

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC015670

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

References (69)