FlyBase Gene Report: Dmel\Atg18a

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Gene: Dmel\Atg18a

General Information

Symbol

Dmel\Atg18a

Species

D. melanogaster

Name

Autophagy-related 18a

Annotation Symbol

CG7986

Feature Type

protein_coding_gene

FlyBase ID

FBgn0035850

Gene Model Status

Stock Availability

16 publicly available

Gene Summary

Autophagy-related 18a (Atg18a) encodes a protein that regulates autophagy by participating in isolation membrane elongation around cytoplasmic cargoes. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

Atg18, Autophagy-specific gene 18

Key Links

Genomic Location

Cytogenetic map

Sequence location

3L:8,186,857..8,191,118 [+]

Recombination map

3-22

RefSeq locus

NT_037436 REGION:8186857..8191118

Sequence

Get Decorated FASTA

Genomic Maps

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (19 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables phosphatidylinositol-3,5-bisphosphate binding

inferred from biological aspect of ancestor with PANTHER:PTN000132136

(GO Reference Genome Project, 2011-)

enables phosphatidylinositol-3-phosphate binding

inferred from biological aspect of ancestor with PANTHER:PTN000132136

(GO Reference Genome Project, 2011-)

enables protein-macromolecule adaptor activity

inferred from biological aspect of ancestor with PANTHER:PTN000132136

(GO Reference Genome Project, 2011-)

Biological Process (14 terms)

Terms Based on Experimental Evidence (10 terms)

CV Term

Evidence

References

involved_in autophagosome assembly

inferred from mutant phenotype

(Nagy et al., 2014)

inferred from mutant phenotype

(Fujita et al., 2017)

involved_in cellular response to starvation

inferred from mutant phenotype

(Nagy et al., 2014, Zirin et al., 2013)

involved_in defense response to virus

inferred from mutant phenotype

(Moy et al., 2014)

involved_in glycophagy

inferred from mutant phenotype

(Zirin et al., 2013)

involved_in larval midgut cell programmed cell death

inferred from mutant phenotype

(Xu et al., 2015, Chang et al., 2013)

involved_in larval midgut histolysis

inferred from mutant phenotype

(Denton et al., 2012, Denton et al., 2009)

involved_in macroautophagy

inferred from mutant phenotype

(Wen et al., 2017, Xu et al., 2015, Chang et al., 2013, Pircs et al., 2012, Scott et al., 2004)

involved_in mitophagy

inferred from direct assay

(Taoka et al., 2025)

inferred from mutant phenotype

(Xu et al., 2019, Chang et al., 2013)

involved_in regulation of defense response to virus

inferred from mutant phenotype

(Shelly et al., 2009)

involved_in T-tubule organization

inferred from mutant phenotype

(Fujita et al., 2017)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

involved_in autophagy of mitochondrion

inferred from biological aspect of ancestor with PANTHER:PTN000132136

(GO Reference Genome Project, 2011-)

involved_in glycophagy

inferred from biological aspect of ancestor with PANTHER:PTN000132136

(GO Reference Genome Project, 2011-)

involved_in nucleophagy

inferred from biological aspect of ancestor with PANTHER:PTN000132136

(GO Reference Genome Project, 2011-)

involved_in pexophagy

inferred from biological aspect of ancestor with PANTHER:PTN000132136

(GO Reference Genome Project, 2011-)

involved_in protein localization to phagophore assembly site

inferred from biological aspect of ancestor with PANTHER:PTN000132136

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in autophagosome

inferred from direct assay

(Vanhauwaert et al., 2017)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in phagophore assembly site membrane

inferred from biological aspect of ancestor with PANTHER:PTN000132136

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

AUTOPHAGY-RELATED GENES

Protein Family (UniProt)

-

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Autophagy-related 18a (Atg18a) encodes a protein that regulates autophagy by participating in isolation membrane elongation around cytoplasmic cargoes. [Date last reviewed: 2019-03-07]

Gene Group (FlyBase)

AUTOPHAGY-RELATED GENES -: The Autophagy-related genes (Atg), characterized extensively in yeast, are a set of approximately twenty evolutionarily conserved genes required for autophagy. Autophagy (specifically, macroautophagy) is the process of bulk degradation of cytoplasmic material. It takes place by the assembly of a double membrane vesicle, the autophagosome, which delivers cargo to the lysosome for recycling. (Adapted from FBrf0219844 and FBrf020902).

Gene Model and Products

Number of Transcripts

7

Number of Unique Polypeptides

4

Please see the JBrowse view of Dmel\Atg18a for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry M9PER1)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.43

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.45

Gene model reviewed during 5.56

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0076691

2097

377

FBtr0076692

2382

377

FBtr0076693

2526

377

FBtr0321319

1980

435

FBtr0321320

2267

447

FBtr0321321

2291

372

FBtr0346547

2235

377

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

FBpp0076414

41.7

377

7.06

FBpp0076415

41.7

377

7.06

FBpp0076416

41.7

377

7.06

FBpp0302862

46.5

435

6.73

FBpp0302863

47.9

447

6.73

FBpp0302864

41.0

372

6.88

FBpp0312165

41.7

377

7.06

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

377 aa isoforms: Atg18a-PA, Atg18a-PB, Atg18a-PC, Atg18a-PG

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Atg18a using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.09

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

central brain anlage in statu nascendi

(Fisher et al., 2012)

dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

ventral ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

antennal anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

dorsal head epidermis anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

visual anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

embryonic stage 7 -- 8

antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal ectoderm

(Fisher et al., 2012)

(Fisher et al., 2012)

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | faint | ubiquitous

(Fisher et al., 2012)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female

(Simonsen et al., 2008)

Additional Descriptive Data

qRT-PCR of adult female heads assayed at weekly intervals shows that transcript levels decline significantly by 3 weeks and remain supressed.

(Simonsen et al., 2008)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in autophagosome

inferred from direct assay

(Vanhauwaert et al., 2017)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Atg18a in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Atg18a

View exonic expression by tissue for Dmel\Atg18a

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0035850

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38913/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0035850

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0035850

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0035850

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7986

Flygut - An atlas of the Drosophila adult midgut

FBgn0035850

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Atg18a

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 13-16

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 8 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 17 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Atg18a

Transgenic constructs containing regulatory region of Atg18a

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

(Ryder, 2004.4.26)

Inferred from location ( 8 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die before end of larval stage | recessive

Atg18a^KG03090

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Atg18a^GD12342

viable, with Scer\GAL4^Mef2.PR

Atg18a^GD12342

viable, with Scer\GAL4^pnr-MD237

Atg18a^GD12342

viable, with Scer\GAL4^tin.CΔ4

Atg18a^GD12342

Other Phenotypes

Allele

abnormal cell cycle | oogenesis, with Scer\GAL4^nanos.PG

Atg18a^HMS01193

abnormal immune response, with Scer\GAL4^Act5C.PU

Atg18a^GD12342

abnormal locomotor behavior | adult stage | male, with Scer\GAL4^Mef2.PU

Atg18a^KK100064

abnormal neuroanatomy

abnormal neuroanatomy | adult stage | somatic clone - tissue specific

Atg18a^KG03090

abnormal neurophysiology | adult stage | somatic clone - tissue specific

Atg18a^KG03090

abnormal size | P-stage, with Scer\GAL4^NP0001

Atg18a^JF02898

abnormal starvation stress response | larval stage, with Scer\GAL4^Cg.PU

Atg18a^JF02898

abnormal stress response | larval stage | recessive

Atg18a^KG03090

abnormal stress response | oogenesis, with Scer\GAL4^nanos.PG

Atg18a^HMS01193

decreased cell death | P-stage, with Scer\GAL4^NP0001

Atg18a^JF02898

decreased cell growth rate

increased size | adult stage, with Scer\GAL4^nub-AC-62

Atg18a^JF02898

short lived, with Scer\GAL4^Act88F.PU

Atg18a^KK100064

short lived, with Scer\GAL4^Mef2.PU

Atg18a^KK100064

short lived, with Scer\GAL4^tin.CΔ4

Atg18a^KK100064

some die during larval stage | recessive

Atg18a^KG03090

visible | adult stage, with Scer\GAL4^nub-AC-62

Atg18a^JF02898

Phenotype manifest in

Allele

adult brain | adult stage | somatic clone - tissue specific

Atg18a^KG03090

adult fat body | nutrition conditional, with Scer\GAL4^Tub.PU

Atg18a^KK100064

adult heart muscle cell | day 40 of adulthood, with Scer\GAL4^Mef2.PU

Atg18a^KK100064

adult heart muscle cell | progressive, with Scer\GAL4^Mef2.PU

Atg18a^KK100064

autophagosome | :chemical conditional | nutrition conditional, with Scer\GAL4^Mef2.PR

Atg18a^JF02898

autophagosome | adult stage | nutrition conditional, with Scer\GAL4^Tub.PU

Atg18a^KK100064

embryonic/larval fat body | larval stage, with Scer\GAL4^Cg.PU

Atg18a^JF02898

embryonic/larval fat body | somatic clone | third instar larval stage, with Scer\GAL4^Act5C.PP

Atg18a^JF02898

embryonic/larval fat body | third instar larval stage

Atg18a^KG03090

embryonic/larval midgut, with Scer\GAL4^NP0001

Atg18a^RNAi.UAS.cUa

embryonic/larval midgut | P-stage, with Scer\GAL4^NP0001

embryonic/larval midgut | P-stage (with Df(3L)Exel6112)

Atg18a^KG03090

embryonic/larval salivary gland (with Df(3L)66C-G28)

Atg18a^KG03090

eye photoreceptor cell | adult stage | somatic clone - tissue specific

Atg18a^KG03090

eye photoreceptor cell | P-stage

Atg18a^KG03090

female germline stem cell, with Scer\GAL4^nanos.PG

Atg18a^HMS01193

filopodium | P-stage | increased number

Atg18a^KG03090

gastric caecum | P-stage, with Scer\GAL4^NP0001

Atg18a^JF02898

indirect flight muscle cell | day 40 of adulthood, with Scer\GAL4^Mef2.PU

Atg18a^KK100064

lysosome | adult stage | nutrition conditional, with Scer\GAL4^Tub.PU

Atg18a^KK100064

mitochondrion | adult stage, with Scer\GAL4^Mef2.PU

Atg18a^KK100064

neuromuscular junction

retina | adult stage | somatic clone - tissue specific

Atg18a^KG03090

synapse | adult stage | increased number | somatic clone - tissue specific

Atg18a^KG03090

wing, with Scer\GAL4^nub-AC-62

Atg18a^JF02898

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (17)

13 of 14

Yes

Yes

1

11 of 14

No

Yes

2 of 14

No

No

2 of 14

No

No

1

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

4

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (11)

13 of 14

Yes

Yes

11 of 14

No

Yes

2 of 14

No

No

2 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

Mus musculus (laboratory mouse) (11)

12 of 14

Yes

Yes

11 of 14

No

Yes

2 of 14

No

No

2 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

Xenopus tropicalis (Western clawed frog) (12)

7 of 13

Yes

Yes

6 of 13

No

Yes

2 of 13

No

No

2 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

Danio rerio (Zebrafish) (13)

11 of 14

Yes

Yes

11 of 14

Yes

Yes

2 of 14

No

No

2 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

Drer\LOC100332293

1 of 14

No

Yes

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

Caenorhabditis elegans (Nematode, roundworm) (9)

12 of 14

Yes

Yes

2 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

Anopheles gambiae (African malaria mosquito) (9)

Agam\AgaP_AGAP005910

11 of 12

Yes

Yes

Agam\AgaP_AGAP007970

6 of 12

No

No

Agam\AgaP_AGAP012792

2 of 12

No

No

Agam\AgaP_AGAP001090

1 of 12

No

No

Agam\AgaP_AGAP004138

1 of 12

No

No

Agam\AgaP_AGAP004280

1 of 12

No

No

Agam\AgaP_AGAP005029

1 of 12

No

No

Agam\AgaP_AGAP009574

1 of 12

No

No

Agam\AgaP_AGAP010693

1 of 12

No

No

Arabidopsis thaliana (thale-cress) (13)

9 of 13

Yes

Yes

3 of 13

No

No

2 of 13

No

No

2 of 13

No

No

2 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

Saccharomyces cerevisiae (Brewer's yeast) (6)

11 of 13

Yes

Yes

6 of 13

No

Yes

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

Schizosaccharomyces pombe (Fission yeast) (10)

11 of 12

Yes

Yes

8 of 12

No

Yes

Spom\SPAC458.06

2 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

Spom\SPBC1A4.07c

1 of 12

No

No

Spom\SPBC3D6.12

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Atg18a. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (5)

11 of 13

3 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

Atg18a^HMS01193

ameliorates C9orf72 frontotemporal dementia and/or amyotrophic lateral sclerosis

modeled by Zzzz\GGGGCC^30.UAS.EGFP

(Cunningham et al., 2020)

exacerbates neurodegenerative disease

modeled by Zzzz\CTG^{rCUG.99.UAS.Tag:MYC,Tag:FLAG}, Zzzz\CAG^{99.UAS.Tag:MYC,Tag:FLAG}

(Samaraweera et al., 2013)

exacerbates carcinoma

modeled by Delta^UAS.cDa, lola^GS88A8

(Pérez et al., 2015)

exacerbates mucopolysaccharidosis type IIIA

modeled by Sgsh^GD1578

(Webber et al., 2018)

Atg18a^RNAi.UAS.cUa

exacerbates Parkinson's disease 6

modeled by Pink1^RNAi.UAS.cYa

(Liu and Lu, 2010)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

WIPI2; WD repeat domain, phosphoinositide interacting 2

13 of 14

INTELLECTUAL DEVELOPMENTAL DISORDER WITH SHORT STATURE AND VARIABLE SKELETAL ANOMALIES; IDDSSA

WIPI1; WD repeat domain, phosphoinositide interacting 1

11 of 14

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

RNA-protein

Physical Interaction

Assay

References

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Rojas-Ríos et al., 2015)

protein-protein

Physical Interaction

Assay

References

anti tag coimmunoprecipitation, anti tag western blot

(Nagy et al., 2014)

anti tag coimmunoprecipitation, anti tag western blot

(Nagy et al., 2014)

anti tag coimmunoprecipitation, anti tag western blot

(Taoka et al., 2025)

Atg18a - ref(2)P

anti tag coimmunoprecipitation, anti tag western blot

(Hegedűs et al., 2014, Nagy et al., 2014)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Denton et al., 2012)

suppressible

(Denton et al., 2012)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Shen and Ganetzky, 2009)

enhanceable

(Simonsen et al., 2007)

suppressible

(Shinoda et al., 2019, Kim et al., 2010)

suppressible

(Shinoda et al., 2019)

suppressible

(Nagy et al., 2013)

enhanceable

(Choo et al., 2015)

suppressible

(Zhu et al., 2015)

suppressible

(Zhu et al., 2017)

suppressible

(Denton et al., 2012)

suppressible

(Tian et al., 2011)

enhanceable

(Webber et al., 2018)

suppressible

(Ikegawa et al., 2023)

suppressible

(Denton et al., 2012)

enhanceable

(Liu et al., 2016)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0035850

MIST (genetic) - An integrated Molecular Interaction Database

38913

MIST (protein-protein) - An integrated Molecular Interaction Database

38913

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Macroautophagy

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9VSF0

Class of Gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

3L

Recombination map

3-22

Cytogenetic map

Sequence location

3L:8,186,857..8,191,118 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

66B11-66B11

Limits computationally determined from genome sequence between P{lacW}Nmt^j1C7 and P{lacW}l(3)L0139^L0139

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-22

(FlyBase, 2016)

3-19.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (16)

Aberrations including deletions of this gene Aberrations including duplications of this gene

28061

y¹ v¹; P{TRiP.JF02898}attP2

80822

y¹, v¹; P{TKO.GS04629}attP40

80430

y¹ v¹; P{TRiP.HMS06010}attP40

82954

y¹ v¹; P{TRiP.HMS06040}attP40

93768

w^*; Atg18a^KO/TM6B, Tb¹

92571

y¹ v¹; P{U6.2-Atg18a.dgRNA}attP40/CyO

34714

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01193}attP2

F002805

M{UAS-Atg18a.ORF.3xHA.GW}ZH-86Fb

HMJ22436

y¹ v¹; P{TRiP.HMJ22436}attP40/CyO

HMS01193

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01193}attP2

v105366

P{KK100064}VIE-260B

v22646

w¹¹¹⁸; P{GD12342}v22646/TM3

More stocks available...

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (55)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD38705

Other clones

DPiM_LD38705

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0035850

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0035850

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA has been made from templates generated with primers directed against this gene.

(Pilot et al., 2006)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Atg18 CG7986

(Scott, 2005.3.2, Scott et al., 2004)

Source for identity of: Atg18a Atg18

(Érdi et al., 2012)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

ATG18

(Song et al., 2020, Shen and Codogno, 2011, Scott, 2005.3.2)

ATG18.1

(Wu et al., 2009)

ATG18a

(Yang et al., 2020)

Atg18

(Bradic and Rewitz, 2025, Demir and Kacew, 2023, Ikegawa et al., 2023, Shekhar et al., 2023, Benoit et al., 2022, Hernandez-Diaz et al., 2022, Murakawa et al., 2022, Rosendo Machado et al., 2021, Denton et al., 2020, Xu et al., 2020, Texada et al., 2019, Xu et al., 2019, Yan et al., 2019, Clark et al., 2018, Kuo et al., 2018, Pandey et al., 2018, Swevers et al., 2018, Webber et al., 2018, Dasari et al., 2017, Kim et al., 2017, Kuleesha et al., 2017, Kutscher and Shaham, 2017, Lei et al., 2017, Wen et al., 2017, Kuleesha et al., 2016, Liu et al., 2016, Puah and Wasser, 2016, Balakrishnan et al., 2015, Choo et al., 2015, Kim et al., 2015, Nagy et al., 2015, Pérez et al., 2015, Rojas-Ríos et al., 2015, Xu et al., 2015, Zirin et al., 2015, Banreti et al., 2014, Hegedűs et al., 2014, Moy et al., 2014, Nagy et al., 2014, Chang et al., 2013, Denton et al., 2013, Samaraweera et al., 2013, Velentzas et al., 2013, Zirin et al., 2013, Denton et al., 2012, Érdi et al., 2012, Jungreis, 2011.11.18, Tian et al., 2011, Chang and Neufeld, 2010, Kim et al., 2010, Liu and Lu, 2010, Denton et al., 2009, Köhler et al., 2009, Shelly et al., 2009, Liu and Lehmann, 2008, Simonsen et al., 2008, Curtis et al., 2007, Zeitouni et al., 2007, Rusten et al., 2006)

Atg18A

(Rojas-Ríos et al., 2015)

Atg18a

(Guo et al., 2025, Krejčová et al., 2025, Li et al., 2025, Mendoza Andrade et al., 2025, Santos-Cruz et al., 2025, Taoka et al., 2025, Zhang et al., 2025, Xu et al., 2024, Oyarce-Pezoa et al., 2023, Ciesielski et al., 2022, Hernandez-Diaz et al., 2022, Huang et al., 2022, Ishii et al., 2022, Jouandin et al., 2022, Xu et al., 2022, Uçkun et al., 2021, Allen et al., 2020, Barthez et al., 2020, Cunningham et al., 2020, Gohel et al., 2020, Jacomin et al., 2020, Murakawa et al., 2020, Rodriguez-Fernandez et al., 2020, Khezri and Rusten, 2019, Meltzer et al., 2019, Yan et al., 2019, Billes et al., 2018, Gene Disruption Project members, 2018-, O'Keefe and Denton, 2018, Xiao et al., 2018, Galluzzi et al., 2017, Manent et al., 2017, Péan et al., 2017, Transgenic RNAi Project members, 2017-, Papp et al., 2016, Zhang and Baehrecke, 2015, Nagy et al., 2013, Érdi et al., 2012, Pircs et al., 2012)

CG7986

(National Institute of Genetics Fly Stocks, 2023-, Trivedi et al., 2020, Érdi et al., 2012, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Wu et al., 2009, Quinones-Coello, 2007, Pilot et al., 2006)

atg18

(Zhao et al., 2023, Zhu et al., 2015, Omata et al., 2014, Kwon et al., 2013, Chen et al., 2012, Nezis, 2012, Milton et al., 2011, Shen and Ganetzky, 2009, Wang et al., 2008, Berry and Baehrecke, 2007, Simonsen et al., 2007)

atg18.1

(Singh and Chowdhuri, 2018)

atg18a

(El Fissi et al., 2024, Joy et al., 2021, Shinoda et al., 2019)

Name Synonyms

Autophagy-related 18a

Autophagy-specific gene 18

(Scott, 2005.3.2, Scott et al., 2004, Scott et al., 2004)

Autophagy-specific gene 18a

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 61 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38913

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

M9PER1

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

M9PER1

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0035850

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38913/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0035850

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0035850

FlyMine - An integrated database for Drosophila genomics

FBgn0035850

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG7986

MARRVEL_MODEL - MARRVEL (model organism gene)

38913

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0035850

DroID - A comprehensive database of gene and protein interactions.

FBgn0035850

DRSC - Results frm RNAi screens

FBgn0035850

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0035850

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0035850

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7986

Flygut - An atlas of the Drosophila adult midgut

FBgn0035850

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0035850

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC006217

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (genetic) - An integrated Molecular Interaction Database

38913

MIST (protein-protein) - An integrated Molecular Interaction Database

38913

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Macroautophagy

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9VSF0

References (171)