FlyBase Allele Report: Dmel\wit[A12]

wit^A12/wit^B11 (but not wit^A12/+) third instar larvae display synaptic undergrowth with fewer mature and satellite boutons at the NMJ than controls.

(Kim et al., 2019)

wit^A12 third instar larval C4da neurons do not show any obvious defects in the dendritic arborization, as compared to controls.

(Hoyer et al., 2018)

wit^B11/wit^A12 transheterozygotes do not reach adulthood and present significant changes in the third instar larval neuromuscular junction compared to controls: a decrease in the number, but not in the area, of synaptic boutons; a significant increase in the active zone density; a significant increase in the total postsynaptic density length; a significant increase in the ruffle density at the synapse; and significant decreases in the subsynaptic reticulum length, but not density.

(Banerjee et al., 2017)

Third instar larvae heterozygous for wit^A12 show no significant differences in the number or size of neuromuscular junction boutons compared to wild-type controls.

(Zhang et al., 2017)

Bouton and branch number at the neuromuscular junction of wit^A12/+ third instar larvae is similar to controls.

(Kashima et al., 2016)

wit^A12/wit^B11 mutant third instar larvae do display synaptic undergrowth (reduced number of boutons) at neuromuscular junctions compared to controls.

(Cho et al., 2015)

wit^A12/wit^B11 mutants show a significant reduction in bouton number at neuromuscular junction 4.

(Liu et al., 2014)

wit^A12/wit^unspecified mutants show a significant reduction in bouton number at muscles 6/7 and 4 NMJs compared to controls.

(Sulkowski et al., 2014)

wit^A12/wit^B11 third instar larvae show a significant decrease in bouton number and satellite bouton number at the neuromuscular junction compared to controls.

(Nahm et al., 2013)

wit^B11/wit^A12 larvae show a reduction in synaptic terminal size at the neuromuscular junction (NMJ) compared to wild type. The frequency and amplitude of the spontaneous miniature excitatory junctional potential (EJP) at the NMJ are decreased compared to wild type.

(Kim and Marqués, 2012)

Heterozygotes do not display defects in neuromuscular junction expansion.

wit^A12/wit^B11 animals display a 46% reduction in bouton number at neuromuscular junction 4.

(James and Broihier, 2011)

wit^A12/wit^HA3 third instar larvae exhibit neuromuscular junction overgrowth and a reduced number of boutons per muscle surface area in muscle 4 compared to controls. Evoked excitatory junctional potentials (EJPs) and quantal content are reduced compared to controls. The size of the miniature excitatory junctional potentials does not differ from controls.

(Ball et al., 2010)

wit^A12/wit^B11 transheterozygotes exhibit approximately half the number of synaptic boutons as found in wild-type terminals.

(Higashi-Kovtun et al., 2010)

wit^A12/wit^B11 third instar larvae display small neuromuscular junctions.

(Khuong et al., 2010)

wit^A12/wit^B11 larvae exhibit synaptic undergrowth.

wit^A12 heterozygotes do not exhibit any synaptic growth defects.

(Nahm et al., 2010)

aCC/RP2 synaptic current amplitude in wit^A12/wit^B11 mutants does not differ from wild-type.

(Fradkin et al., 2008)

wit^A12/wit^B11 larvae show a decrease in the number of type 1b boutons at muscle 13 compared to wild type.

(Haussmann et al., 2008)

The average total bouton number at the neuromuscular junction is decreased in wit^A12/wit^B11 larvae compared to controls.

(O'Connor-Giles et al., 2008)

wit^A12/wit^B11 third instar larvae show a reduction in synaptic growth at the neuromuscular junction. The mean bouton number for segment A3, muscle 6/7 is reduced compared to wild type, and fewer satellite boutons are seen in segments A2-A3, muscle 4 than in controls.

(Rodal et al., 2008)

wit^A12/wit^B11 mutants exhibit a dramatic reduction in bouton number (64%), branching (80%), and synaptic area (64%).

(Collins et al., 2006)

wit^A12/wit^B11 trans-heterozygous neuromuscular junctions exhibit very low EJP amplitudes and low quantal content, but maintain wild-type level mEJP amplitudes.

(van der Plas et al., 2006)

At 25^oC less than 0.5% of wit^A12/wit^B11 animals escape from the pupal case.

(Marques et al., 2003)

wit^A12/wit^C175 transheterozygous mutants are completely lethal. Approximately 50% of animals survive to the pharate stage. The remaining animals die during larval and early pupal stages. Those animals that do survive to pharate adult stage exhibit the head expansion and abdominal peristaltic movements characteristic of normal pre-eclosion behaviour, but do not rupture nor escape from the pupal case. Many pharate adults show a bend in the femur or tibia, the phenotype being more pronounced in the metathoracic leg. A small fraction of the animals also exhibit truncations of the tarsal segments. The general organisation of the nervous system in these mutants is essentially normal. wit^A12/wit^C175 mutant larvae exhibit dramatically reduced transmitter release from presynaptic terminals when the excitatory junctional current (EJC) of body wall muscles examined. The amplitude of EJCs at 0.8 mM Ca²⁺ is significantly reduced in mutants. Most nerve stimuli fail to evoke transmitter release at low extracellular ^Ca<up>2+</up> in mutants. Cooperativity is unaffected in mutants. The amplitude of miniature excitatory junction potentials (mEJPs) is not significantly altered in mutants. The number of synaptic boutons seen in ventral longitudinal muscles 6 and 7 in wit^A12/wit^C175 third instar larvae, are about half that seen in wild-type animals, when corrected for muscle size the number of synaptic boutons seen is about 70% of wild-type. Although synapse size and bouton number are reduced in mutants, the overall percentage reduction is not proportionately as severe as the reduction in EJC magnitude. Bouton size and overall synaptic structure appear normal. However there is a dramatic alteration in the morphology of the active zone. The pre- and postsynaptic membranes are no longer closely aligned. The presynaptic membrane detaches completely from the postsynaptic membrane, forming large curvatures bending toward the cytosol of nerve terminals. Such membrane detachments also occur in areas near the active zones. However presynaptic membranes do not separate from postsynaptic membranes.

(Marques et al., 2002)

wit^A12/wit^B11 larvae show a significant decrease in bouton number at the neuromuscular junction.

(Sweeney and Davis, 2002)

External Data

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Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

wit^B11/wit^A12 has abnormal neuroanatomy | larval stage phenotype, enhanceable by Scer\GAL4^elav.PU/twit^UAS.cKa

(Kim and Marqués, 2012)

wit^A12 has abnormal neuroanatomy phenotype, enhanceable by Impβ11⁷⁰

(Higashi-Kovtun et al., 2010)

NOT Enhanced by

Statement

Reference

wit^B11/wit^A12 has abnormal neuroanatomy | larval stage phenotype, non-enhanceable by Megf8^HSC/Megf8^HSC

(Chen et al., 2022)

wit^B11/wit^A12 has abnormal neuroanatomy phenotype, non-enhanceable by WASp^GD1559/Scer\GAL4^C57

(Nahm et al., 2010)

wit^B11/wit^A12 has abnormal neuroanatomy phenotype, non-enhanceable by Cip4^NIG.15015R/Scer\GAL4^C57

(Nahm et al., 2010)

wit^B11/wit^A12 has abnormal neurophysiology phenotype, non-enhanceable by Scer\GAL4^G14/Dys^RNAi.NH2.UAS

(van der Plas et al., 2006)

Suppressed by

Statement

Reference

wit^HA3/wit^A12 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^BG380/trio^UAS.cBa

(Ball et al., 2010)

wit^B11/wit^A12 has lethal phenotype, suppressible | partially by FMRFa^UAS.cMa/Scer\GAL4^dimm-929

(Marques et al., 2003)

NOT suppressed by

Statement

Reference

wit^B11/wit^A12 has abnormal neuroanatomy | larval stage phenotype, non-suppressible by Megf8^HSC/Megf8^HSC

(Chen et al., 2022)

wit^B11/wit^A12 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Abi⁵/Df(3R)su(Hw)7

(Kim et al., 2019)

wit^B11/wit^A12 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Abl⁴/Abl¹

(Kim et al., 2019)

wit^B11/wit^A12 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/Abi^UAS.Tag:HA

(Kim et al., 2019)

wit^B11/wit^A12 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/CG41099^HMS01228

(Kim et al., 2019)

wit^B11/wit^A12 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/CtBP^HMS00677

(Kim et al., 2019)

wit^B11/wit^A12 has lethal - all die before end of P-stage phenotype, non-suppressible by Scer\GAL4^CCAP.PP/Mip^UAS.cVa/CCAP^UAS.cVa/Pburs^UAS.cVa

(Veverytsa and Allan, 2011)

wit^B11/wit^A12 has some die during larval stage phenotype, non-suppressible by Scer\GAL4^CCAP.PP/Mip^UAS.cVa/CCAP^UAS.cVa/Pburs^UAS.cVa

(Veverytsa and Allan, 2011)

wit^HA3/wit^A12 has abnormal size | third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^UAS.Tag:MYC

(Ball et al., 2010)

wit^HA3/wit^A12 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^UAS.Tag:MYC

(Ball et al., 2010)

wit^HA3/wit^A12 has abnormal size | third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^V12.UAS

(Ball et al., 2010)

wit^HA3/wit^A12 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^V12.UAS

(Ball et al., 2010)

wit^HA3/wit^A12 has abnormal neurophysiology | third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^UAS.cLa

(Ball et al., 2010)

wit^B11/wit^A12 has abnormal neuroanatomy phenotype, non-suppressible by Cip4^NIG.15015R/Scer\GAL4^C57

(Nahm et al., 2010)

wit^B11/wit^A12 has abnormal neuroanatomy phenotype, non-suppressible by WASp^GD1559/Scer\GAL4^C57

(Nahm et al., 2010)

wit^A12 has abnormal size | third instar larval stage phenotype, non-suppressible by nwk¹

(Rodal et al., 2008)

wit^B11/wit^A12 has abnormal neurophysiology phenotype, non-suppressible by Scer\GAL4^G14/Dys^RNAi.NH2.UAS

(van der Plas et al., 2006)

wit^B11/wit^A12 has abnormal neurophysiology phenotype, non-suppressible by FMRFa^UAS.cMa/Scer\GAL4^dimm-929

(Marques et al., 2003)

Enhancer of

Statement

Reference

wit^A12/wit[+] is an enhancer of abnormal neuroanatomy phenotype of Impβ11⁷⁰

(Higashi-Kovtun et al., 2010)

NOT Enhancer of

Statement

Reference

wit^B11/wit^A12 is a non-enhancer of abnormal neuroanatomy | larval stage | recessive phenotype of Megf8^HSC

(Chen et al., 2022)

wit^A12/wit[+] is a non-enhancer of visible | adult stage phenotype of HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU

(Padash Barmchi et al., 2016)

wit^B11/wit^A12 is a non-enhancer of abnormal neurophysiology phenotype of Dys^{RNAi.Dp186.UAS}, Scer\GAL4^eve.RN2

(Fradkin et al., 2008)

Suppressor of

Statement

Reference

wit^B11/wit^A12 is a suppressor | partially of abnormal neuroanatomy | larval stage phenotype of Scer\GAL4^elav.PU, Sec15^KK101708

(Kang et al., 2024)

wit^A12 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Abi⁵/Df(3R)su(Hw)7

(Kim et al., 2019)

wit^A12 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Abl⁴/Abl¹

(Kim et al., 2019)

wit^A12/wit[+] is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of CG41099^HMS01228, Scer\GAL4^elav-C155

(Kim et al., 2019)

wit^A12/wit[+] is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of CtBP^HMS00677, Scer\GAL4^elav-C155

(Kim et al., 2019)

wit^A12 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Rac1^N17.UAS, Scer\GAL4^elav-C155

(Kim et al., 2019)

wit^B11/wit^A12 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Rac1^N17.UAS, Scer\GAL4^elav-C155

(Kim et al., 2019)

wit^A12/wit[+] is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Nlg4^UAS.cZa, Scer\GAL4^elav.PU

(Zhang et al., 2017)

wit^B11/wit^A12 is a suppressor of abnormal neuroanatomy | dominant | third instar larval stage phenotype of Fmr1^Δ113M

(Kashima et al., 2016)

wit^A12/wit[+] is a suppressor of abnormal neuroanatomy | dominant | third instar larval stage phenotype of Fmr1^Δ113M

(Kashima et al., 2016)

wit^A12/wit[+] is a suppressor of abnormal neuroanatomy | larval stage phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO

(Liu et al., 2014)

wit^A12/wit[+] is a suppressor of abnormal neurophysiology | third instar larval stage phenotype of spartin¹/Df(3R)110

(Nahm et al., 2013)

wit^A12/wit[+] is a suppressor of abnormal neuroanatomy | adult stage | progressive phenotype of spartin¹/Df(3R)110

(Nahm et al., 2013)

wit^B11/wit^A12 is a suppressor of abnormal neuroanatomy | larval stage phenotype of Scer\GAL4^elav-C155, Snx16^3A.UAS.GFP

(Rodal et al., 2011)

wit^HA3/wit^A12 is a suppressor of abnormal neurophysiology | third instar larval stage phenotype of Rac1^UAS.cLa, Scer\GAL4^BG380

(Ball et al., 2010)

wit^HA3/wit^A12 is a suppressor of abnormal size | third instar larval stage phenotype of Rac1^UAS.Tag:MYC, Scer\GAL4^BG380

(Ball et al., 2010)

wit^HA3/wit^A12 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Rac1^UAS.Tag:MYC, Scer\GAL4^BG380

(Ball et al., 2010)

wit^A12/wit[+] is a suppressor of abnormal neuroanatomy phenotype of Cip4^NIG.15015R, Scer\GAL4^C57

(Nahm et al., 2010)

wit^A12/wit[+] is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^C57, WASp^GD1559

(Nahm et al., 2010)

wit^A12/wit[+] is a suppressor of abnormal neuroanatomy | larval stage phenotype of nwk¹/nwk²

(O'Connor-Giles et al., 2008)

wit^A12/wit[+] is a suppressor of abnormal neuroanatomy | larval stage phenotype of Dap160^Δ1

(O'Connor-Giles et al., 2008)

wit^B11/wit^A12 is a suppressor of abnormal size | third instar larval stage phenotype of nwk¹

(Rodal et al., 2008)

wit^B11/wit^A12 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of nwk¹

(Rodal et al., 2008)

wit^B11/wit^A12 is a suppressor of abnormal neuroanatomy phenotype of spict^mut

(Wang et al., 2007)

wit^A12/wit[+] is a suppressor | partially of abnormal neuroanatomy phenotype of spict^mut

(Wang et al., 2007)

wit^A12/wit[+] is a suppressor of abnormal neuroanatomy phenotype of spin^k09905/spin¹⁰⁴⁰³

(Sweeney and Davis, 2002)

wit^B11/wit^A12 is a suppressor of abnormal neuroanatomy phenotype of spin^k09905/spin¹⁰⁴⁰³

(Sweeney and Davis, 2002)

NOT Suppressor of

Statement

Reference

wit^B11/wit^A12 is a non-suppressor of abnormal neuroanatomy | recessive | larval stage phenotype of Megf8^HSC

(Chen et al., 2022)

wit^A12/wit[+] is a non-suppressor of abnormal neurophysiology | third instar larval stage phenotype of cpx^SH1

(Cho et al., 2015)

wit^B11/wit^A12 is a non-suppressor of abnormal neurophysiology | third instar larval stage phenotype of cpx^SH1

(Cho et al., 2015)

wit^A12/wit[+] is a non-suppressor of abnormal neuroanatomy | third instar larval stage phenotype of brat^2L-192-9/brat¹¹

(Shi et al., 2013)

wit^HA3/wit^A12 is a non-suppressor of abnormal size | third instar larval stage phenotype of Rac1^V12.UAS, Scer\GAL4^BG380

(Ball et al., 2010)

wit^HA3/wit^A12 is a non-suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Rac1^V12.UAS, Scer\GAL4^BG380

(Ball et al., 2010)

wit^B11/wit^A12 is a non-suppressor of abnormal neurophysiology phenotype of Dys^{RNAi.Dp186.UAS}, Scer\GAL4^eve.RN2

(Fradkin et al., 2008)

Other

Statement

Reference

Megf8^HSC, wit^A12 has abnormal neuroanatomy | larval stage phenotype

(Chen et al., 2022)

Rac1^N17.UAS, Scer\GAL4^elav-C155, wit^A12 has abnormal neuroanatomy | third instar larval stage phenotype

(Kim et al., 2019)

Rac1^N17.UAS, Scer\GAL4^elav-C155, wit^B11/wit^A12 has abnormal neuroanatomy | third instar larval stage phenotype

(Kim et al., 2019)

Nlg4^KO10, wit^A12/wit[+] has abnormal neuroanatomy | third instar larval stage phenotype

(Zhang et al., 2017)

Fmr1^Δ113M/Fmr1[+], wit^A12 has abnormal neuroanatomy | third instar larval stage phenotype

(Kashima et al., 2016)

Acsl⁰⁵⁸⁴⁷/Acsl^KO, wit^B11/wit^A12 has abnormal neuroanatomy | larval stage phenotype

(Liu et al., 2014)

Scer\GAL4^CCAP.PP, sax^CA.UAS.cUa, tkv^CA.UAS.cUa, wit^B11/wit^A12 has lethal - all die before end of P-stage phenotype

(Veverytsa and Allan, 2011)

Scer\GAL4^CCAP.PP, sax^CA.UAS.cUa, tkv^CA.UAS.cUa, wit^B11/wit^A12 has majority die during larval stage phenotype

(Veverytsa and Allan, 2011)

CCAP^UAS.cVa, Mip^UAS.cVa, Pburs^UAS.cVa, Scer\GAL4^CCAP.PP, wit^B11/wit^A12 has decreased body size phenotype

(Veverytsa and Allan, 2011)

RhoGAP92B¹, wit^A12/wit[+] has abnormal neuroanatomy | dominant | third instar larval stage phenotype

(Nahm et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

wit^B11/wit^A12 has embryonic/larval neuromuscular junction phenotype, enhanceable by Scer\GAL4^elav.PU/twit^UAS.cKa

(Kim and Marqués, 2012)

wit^A12 has bouton phenotype, enhanceable by Ranbp11[+]/Impβ11⁷⁰

(Higashi-Kovtun et al., 2010)

NOT Enhanced by

Statement

Reference

wit^B11/wit^A12 has NMJ bouton | larval stage | decreased number phenotype, non-enhanceable by Megf8^HSC/Megf8^HSC

(Chen et al., 2022)

wit^B11/wit^A12 has neuromuscular junction | third instar larval stage phenotype, non-enhanceable by Nlg1^Δ46/Nlg1^Δ46

(Banerjee et al., 2017)

wit^B11/wit^A12 has terminal bouton | third instar larval stage phenotype, non-enhanceable by Nlg1^Δ46/Nlg1^Δ46

(Banerjee et al., 2017)

wit^B11/wit^A12 has presynaptic active zone | third instar larval stage phenotype, non-enhanceable by Nlg1^Δ46/Nlg1^Δ46

(Banerjee et al., 2017)

wit^B11/wit^A12 has postsynaptic density | third instar larval stage phenotype, non-enhanceable by Nlg1^Δ46/Nlg1^Δ46

(Banerjee et al., 2017)

wit^B11/wit^A12 has subsynaptic reticulum | third instar larval stage phenotype, non-enhanceable by Nlg1^Δ46/Nlg1^Δ46

(Banerjee et al., 2017)

wit^B11/wit^A12 has neuromuscular junction | third instar larval stage phenotype, non-enhanceable by Nrx-1²⁷³/Nrx-1²⁷³

(Banerjee et al., 2017)

wit^B11/wit^A12 has terminal bouton | third instar larval stage phenotype, non-enhanceable by Nrx-1²⁷³/Nrx-1²⁷³

(Banerjee et al., 2017)

wit^B11/wit^A12 has presynaptic active zone | third instar larval stage phenotype, non-enhanceable by Nrx-1²⁷³/Nrx-1²⁷³

(Banerjee et al., 2017)

wit^B11/wit^A12 has postsynaptic density | third instar larval stage phenotype, non-enhanceable by Nrx-1²⁷³/Nrx-1²⁷³

(Banerjee et al., 2017)

wit^B11/wit^A12 has subsynaptic reticulum | third instar larval stage phenotype, non-enhanceable by Nrx-1²⁷³/Nrx-1²⁷³

(Banerjee et al., 2017)

wit^B11/wit^A12 has neuromuscular junction phenotype, non-enhanceable by WASp^GD1559/Scer\GAL4^C57

(Nahm et al., 2010)

wit^B11/wit^A12 has synapse phenotype, non-enhanceable by Cip4^NIG.15015R/Scer\GAL4^C57

(Nahm et al., 2010)

wit^B11/wit^A12 has neuromuscular junction phenotype, non-enhanceable by Cip4^NIG.15015R/Scer\GAL4^C57

(Nahm et al., 2010)

wit^B11/wit^A12 has synapse phenotype, non-enhanceable by WASp^GD1559/Scer\GAL4^C57

(Nahm et al., 2010)

Suppressed by

Statement

Reference

wit^B11/wit^A12 has pupa phenotype, suppressible | partially by Scer\GAL4^CCAP.PP/tkv^CA.UAS.cUa/sax^CA.UAS.cUa

(Veverytsa and Allan, 2011)

wit^B11/wit^A12 has pupa phenotype, suppressible | partially by Scer\GAL4^CCAP.PP/CCAP^UAS.cVa

(Veverytsa and Allan, 2011)

wit^B11/wit^A12 has pupa phenotype, suppressible | partially by Scer\GAL4^CCAP.PP/Pburs^UAS.cVa

(Veverytsa and Allan, 2011)

wit^B11/wit^A12 has pupa phenotype, suppressible | partially by Scer\GAL4^CCAP.PP/Mip^UAS.cVa

(Veverytsa and Allan, 2011)

wit^B11/wit^A12 has pupa phenotype, suppressible | partially by Scer\GAL4^CCAP.PP/Mip^UAS.cVa/Pburs^UAS.cVa

(Veverytsa and Allan, 2011)

wit^B11/wit^A12 has pupa phenotype, suppressible | partially by Scer\GAL4^CCAP.PP/CCAP^UAS.cVa/Pburs^UAS.cVa

(Veverytsa and Allan, 2011)

wit^B11/wit^A12 has pupa phenotype, suppressible | partially by Scer\GAL4^CCAP.PP/Mip^UAS.cVa/CCAP^UAS.cVa

(Veverytsa and Allan, 2011)

wit^B11/wit^A12 has pupa phenotype, suppressible | partially by Scer\GAL4^CCAP.PP/Mip^UAS.cVa/CCAP^UAS.cVa/Pburs^UAS.cVa

(Veverytsa and Allan, 2011)

wit^HA3/wit^A12 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^BG380/trio^UAS.cBa

(Ball et al., 2010)

wit^HA3/wit^A12 has NMJ bouton | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^BG380/trio^UAS.cBa

(Ball et al., 2010)

wit^B11/wit^A12 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible | partially by Df(3R)3450/WASp¹

(Khuong et al., 2010)

NOT suppressed by

Statement

Reference

wit^B11/wit^A12 has NMJ bouton | larval stage | decreased number phenotype, non-suppressible by Megf8^HSC/Megf8^HSC

(Chen et al., 2022)

wit^B11/wit^A12 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-suppressible by Abi⁵/Df(3R)su(Hw)7

(Kim et al., 2019)

wit^B11/wit^A12 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-suppressible by Abl⁴/Abl¹

(Kim et al., 2019)

wit^B11/wit^A12 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/Abi^UAS.Tag:HA

(Kim et al., 2019)

wit^B11/wit^A12 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/CG41099^HMS01228

(Kim et al., 2019)

wit^B11/wit^A12 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/CtBP^HMS00677

(Kim et al., 2019)

wit^B11/wit^A12 has NMJ bouton | third instar larval stage phenotype, non-suppressible by Abi⁵/Df(3R)su(Hw)7

(Kim et al., 2019)

wit^B11/wit^A12 has NMJ bouton | third instar larval stage phenotype, non-suppressible by Abl⁴/Abl¹

(Kim et al., 2019)

wit^B11/wit^A12 has NMJ bouton | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/Abi^UAS.Tag:HA

(Kim et al., 2019)

wit^B11/wit^A12 has NMJ bouton | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/CG41099^HMS01228

(Kim et al., 2019)

wit^B11/wit^A12 has NMJ bouton | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/CtBP^HMS00677

(Kim et al., 2019)

wit^B11/wit^A12 has neuromuscular junction | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav.PU/Nrx-1^UAS.cLa

(Banerjee et al., 2017)

wit^B11/wit^A12 has terminal bouton | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav.PU/Nrx-1^UAS.cLa

(Banerjee et al., 2017)

wit^HA3/wit^A12 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^UAS.Tag:MYC

(Ball et al., 2010)

wit^HA3/wit^A12 has NMJ bouton | third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^UAS.Tag:MYC

(Ball et al., 2010)

wit^HA3/wit^A12 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^V12.UAS

(Ball et al., 2010)

wit^HA3/wit^A12 has NMJ bouton | third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^V12.UAS

(Ball et al., 2010)

wit^B11/wit^A12 has synapse phenotype, non-suppressible by Cip4^NIG.15015R/Scer\GAL4^C57

(Nahm et al., 2010)

wit^B11/wit^A12 has neuromuscular junction phenotype, non-suppressible by Cip4^NIG.15015R/Scer\GAL4^C57

(Nahm et al., 2010)

wit^B11/wit^A12 has synapse phenotype, non-suppressible by WASp^GD1559/Scer\GAL4^C57

(Nahm et al., 2010)

wit^B11/wit^A12 has neuromuscular junction phenotype, non-suppressible by WASp^GD1559/Scer\GAL4^C57

(Nahm et al., 2010)

wit^A12 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-suppressible by nwk¹

(Rodal et al., 2008)

wit^A12 has NMJ bouton | third instar larval stage phenotype, non-suppressible by nwk¹

(Rodal et al., 2008)

wit^B11/wit^A12 has synapse phenotype, non-suppressible by FMRFa^UAS.cMa/Scer\GAL4^dimm-929

(Marques et al., 2003)

Enhancer of

Statement

Reference

wit^B11/wit^A12 is an enhancer of neuromuscular junction | third instar larval stage phenotype of Nrx-1²⁷³

(Banerjee et al., 2017)

wit^B11/wit^A12 is an enhancer of terminal bouton | third instar larval stage phenotype of Nrx-1²⁷³

(Banerjee et al., 2017)

wit^B11/wit^A12 is an enhancer of neuromuscular junction | third instar larval stage phenotype of Nlg1^Δ46

(Banerjee et al., 2017)

wit^B11/wit^A12 is an enhancer of terminal bouton | third instar larval stage phenotype of Nlg1^Δ46

(Banerjee et al., 2017)

wit^A12/wit[+] is an enhancer of bouton phenotype of Impβ11⁷⁰

(Higashi-Kovtun et al., 2010)

wit^B11/wit^A12 is an enhancer of NMJ bouton | third instar larval stage phenotype of nwk¹

(Rodal et al., 2008)

NOT Enhancer of

Statement

Reference

wit^B11/wit^A12 is a non-enhancer of NMJ bouton | larval stage | decreased number phenotype of Megf8^HSC

(Chen et al., 2022)

wit^A12/wit[+] is a non-enhancer of eye phenotype of HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU

(Padash Barmchi et al., 2016)

Suppressor of

Statement

Reference

wit^B11/wit^A12 is a suppressor | partially of embryonic/larval neuromuscular junction | larval stage phenotype of Scer\GAL4^elav.PU, Sec15^KK101708

(Kang et al., 2024)

wit^A12 is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Abi⁵/Df(3R)su(Hw)7

(Kim et al., 2019)

wit^A12 is a suppressor of NMJ bouton | third instar larval stage phenotype of Abi⁵/Df(3R)su(Hw)7

(Kim et al., 2019)

wit^A12 is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Abl⁴/Abl¹

(Kim et al., 2019)

wit^A12 is a suppressor of NMJ bouton | third instar larval stage phenotype of Abl⁴/Abl¹

(Kim et al., 2019)

wit^A12/wit[+] is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of CG41099^HMS01228, Scer\GAL4^elav-C155

(Kim et al., 2019)

wit^A12/wit[+] is a suppressor of NMJ bouton | third instar larval stage phenotype of CG41099^HMS01228, Scer\GAL4^elav-C155

(Kim et al., 2019)

wit^A12/wit[+] is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of CtBP^HMS00677, Scer\GAL4^elav-C155

(Kim et al., 2019)

wit^A12/wit[+] is a suppressor of NMJ bouton | third instar larval stage phenotype of CtBP^HMS00677, Scer\GAL4^elav-C155

(Kim et al., 2019)

wit^A12 is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Rac1^N17.UAS, Scer\GAL4^elav-C155

(Kim et al., 2019)

wit^B11/wit^A12 is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Rac1^N17.UAS, Scer\GAL4^elav-C155

(Kim et al., 2019)

wit^A12 is a suppressor of NMJ bouton | third instar larval stage phenotype of Rac1^N17.UAS, Scer\GAL4^elav-C155

(Kim et al., 2019)

wit^B11/wit^A12 is a suppressor of NMJ bouton | third instar larval stage phenotype of Rac1^N17.UAS, Scer\GAL4^elav-C155

(Kim et al., 2019)

wit^B11/wit^A12 is a suppressor of neuromuscular junction | third instar larval stage phenotype of Nrx-1^UAS.cLa, Scer\GAL4^elav.PU

(Banerjee et al., 2017)

wit^B11/wit^A12 is a suppressor of terminal bouton | third instar larval stage phenotype of Nrx-1^UAS.cLa, Scer\GAL4^elav.PU

(Banerjee et al., 2017)

wit^A12/wit[+] is a suppressor of NMJ bouton | increased number | third instar larval stage phenotype of Nlg4^UAS.cZa, Scer\GAL4^RapGAP1-OK6

(Zhang et al., 2017)

wit^A12/wit[+] is a suppressor of NMJ bouton | increased number | third instar larval stage phenotype of Fmr1^Δ113M

(Kashima et al., 2016)

wit^A12/wit[+] is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Fmr1^Δ113M

(Kashima et al., 2016)

wit^B11/wit^A12 is a suppressor of NMJ bouton | increased number | third instar larval stage phenotype of Fmr1^Δ113M

(Kashima et al., 2016)

wit^B11/wit^A12 is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Fmr1^Δ113M

(Kashima et al., 2016)

wit^A12/wit[+] is a suppressor of neuromuscular junction | third instar larval stage phenotype of cpx^SH1

(Cho et al., 2015)

wit^A12/wit[+] is a suppressor of NMJ bouton | third instar larval stage phenotype of cpx^SH1

(Cho et al., 2015)

wit^B11/wit^A12 is a suppressor of neuromuscular junction | third instar larval stage phenotype of cpx^SH1

(Cho et al., 2015)

wit^B11/wit^A12 is a suppressor of NMJ bouton | third instar larval stage phenotype of cpx^SH1

(Cho et al., 2015)

wit^A12/wit[+] is a suppressor of embryonic/larval neuromuscular junction phenotype of S6KL¹⁴⁰

(Zhao et al., 2015)

wit^A12/wit[+] is a suppressor of NMJ bouton | increased number phenotype of S6KL¹⁴⁰

(Zhao et al., 2015)

wit^A12/wit[+] is a suppressor of neuromuscular junction phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO

(Liu et al., 2014)

wit^A12/wit[+] is a suppressor of NMJ bouton | increased number phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO

(Liu et al., 2014)

wit^A12/wit[+] is a suppressor of larval abdominal 2 neuron phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO

(Liu et al., 2014)

wit^A12/wit[+] is a suppressor of larval abdominal 3 neuron phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO

(Liu et al., 2014)

wit^A12/wit[+] is a suppressor of NMJ bouton | increased number | third instar larval stage phenotype of spartin¹/Df(3R)110

(Nahm et al., 2013)

wit^A12/wit[+] is a suppressor of adult brain | progressive phenotype of spartin¹/Df(3R)110

(Nahm et al., 2013)

wit^A12/wit[+] is a suppressor | partially of type I bouton | increased number | third instar larval stage phenotype of cmpy^Δ8

(James and Broihier, 2011)

wit^B11/wit^A12 is a suppressor of NMJ bouton | increased number | larval stage phenotype of Scer\GAL4^elav-C155, Snx16^3A.UAS.GFP

(Rodal et al., 2011)

wit^HA3/wit^A12 is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Rac1^UAS.Tag:MYC, Scer\GAL4^BG380

(Ball et al., 2010)

wit^HA3/wit^A12 is a suppressor of NMJ bouton | third instar larval stage phenotype of Rac1^UAS.Tag:MYC, Scer\GAL4^BG380

(Ball et al., 2010)

wit^A12/wit[+] is a suppressor of neuromuscular junction phenotype of Cip4^NIG.15015R, Scer\GAL4^C57

(Nahm et al., 2010)

wit^A12/wit[+] is a suppressor of bouton phenotype of Cip4^NIG.15015R, Scer\GAL4^C57

(Nahm et al., 2010)

wit^A12/wit[+] is a suppressor of neuromuscular junction phenotype of Scer\GAL4^C57, WASp^GD1559

(Nahm et al., 2010)

wit^A12/wit[+] is a suppressor of bouton phenotype of Scer\GAL4^C57, WASp^GD1559

(Nahm et al., 2010)

wit^A12/wit[+] is a suppressor of NMJ bouton | increased number | larval stage phenotype of nwk¹/nwk²

(O'Connor-Giles et al., 2008)

wit^A12/wit[+] is a suppressor of NMJ bouton | increased number | larval stage phenotype of Dap160^Δ1

(O'Connor-Giles et al., 2008)

wit^B11/wit^A12 is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of nwk¹

(Rodal et al., 2008)

wit^B11/wit^A12 is a suppressor of embryonic/larval neuromuscular junction phenotype of spict^mut

(Wang et al., 2007)

wit^A12/wit[+] is a suppressor | partially of embryonic/larval neuromuscular junction phenotype of spict^mut

(Wang et al., 2007)

wit^A12/wit[+] is a suppressor of bouton | increased number phenotype of spin^k09905/spin¹⁰⁴⁰³

(Sweeney and Davis, 2002)

wit^B11/wit^A12 is a suppressor of bouton | increased number phenotype of spin^k09905/spin¹⁰⁴⁰³

(Sweeney and Davis, 2002)

wit^A12/wit[+] is a suppressor of neuromuscular junction phenotype of spin^k09905/spin¹⁰⁴⁰³

(Sweeney and Davis, 2002)

wit^B11/wit^A12 is a suppressor of neuromuscular junction phenotype of spin^k09905/spin¹⁰⁴⁰³

(Sweeney and Davis, 2002)

NOT Suppressor of

Statement

Reference

wit^B11/wit^A12 is a non-suppressor of NMJ bouton | larval stage | decreased number phenotype of Megf8^HSC

(Chen et al., 2022)

wit^A12/wit[+] is a non-suppressor of NMJ bouton | increased number | third instar larval stage phenotype of brat^2L-192-9/brat¹¹

(Shi et al., 2013)

wit^HA3/wit^A12 is a non-suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Rac1^V12.UAS, Scer\GAL4^BG380

(Ball et al., 2010)

wit^HA3/wit^A12 is a non-suppressor of NMJ bouton | third instar larval stage phenotype of Rac1^V12.UAS, Scer\GAL4^BG380

(Ball et al., 2010)

Other

Statement

Reference

Megf8^HSC, wit^A12 has NMJ bouton | larval stage | decreased number phenotype

(Chen et al., 2022)

Rac1^N17.UAS, Scer\GAL4^elav-C155, wit^A12 has NMJ bouton | third instar larval stage phenotype

(Kim et al., 2019)

Rac1^N17.UAS, Scer\GAL4^elav-C155, wit^A12 has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Kim et al., 2019)

Rac1^N17.UAS, Scer\GAL4^elav-C155, wit^B11/wit^A12 has NMJ bouton | third instar larval stage phenotype

(Kim et al., 2019)

Rac1^N17.UAS, Scer\GAL4^elav-C155, wit^B11/wit^A12 has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Kim et al., 2019)

Nlg4^KO10, wit^A12/wit[+] has NMJ bouton | third instar larval stage phenotype

(Zhang et al., 2017)

Fmr1^Δ113M/Fmr1[+], wit^A12 has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Kashima et al., 2016)

Fmr1^Δ113M/Fmr1[+], wit^A12 has NMJ bouton | increased number | third instar larval stage phenotype

(Kashima et al., 2016)

Acsl⁰⁵⁸⁴⁷/Acsl^KO, wit^B11/wit^A12 has NMJ bouton phenotype

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO, wit^B11/wit^A12 has neuromuscular junction phenotype

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO, wit^B11/wit^A12 has larval abdominal 2 neuron phenotype

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl^KO, wit^B11/wit^A12 has larval abdominal 3 neuron phenotype

(Liu et al., 2014)

cmpy^Δ8, wit^A12 has type I bouton | third instar larval stage phenotype

(James and Broihier, 2011)

RhoGAP92B¹, wit^A12/wit[+] has NMJ bouton | third instar larval stage phenotype

(Nahm et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Expressing Rac1^N17.UAS under the control of Scer\GAL4^elav-C155 in a wit^A12 background results in significantly fewer satellite boutons, but a similar number of mature synaptic boutons as controls at the NMJ of third instar larvae. The number of both bouton types is significantly reduced when expression is driven in a wit^A12/wit^B11 background.

(Kim et al., 2019)

The third instar larval neuromuscular junction defects observed in wit^B11/wit^A12 transheterozygotes is not suppressed or enhanced by either Nrx-1²⁷³ or Nlg1^Δ46 homozygosity.

The third instar larval neuromuscular junctions of individuals that both express Nrx-1^Scer\UAS.cLa under the control of Scer\GAL4^elav.PU and are wit^B11/wit^A12 transheterozygous show a decrease in the number of synaptic boutons similar to what is observed in wit^B11/wit^A12 transheterozygotes, rather than the increase induced by the expression of Nrx-1^Scer\UAS.cLa.

(Banerjee et al., 2017)

The increased bouton and satellite bouton number characteristic for third instar larvae expressing two copies of Nlg4^Scer\UAS.cZa under the control of Scer\GAL4^RapGAP1-OK6 is suppressed by combination with a single copy of wit^A12.

Although none of the larvae heterozygous for either Nlg4^KO10 or wit^A12 display any significant difference in the number or size of boutons on neuromuscular junctions compared to controls, wit^A12,Nlg4^KO10 double heterozygotes show reduced bouton number and increased bouton size.

(Zhang et al., 2017)

Syx4⁷³/+;wit^A12/+ double heterozygotic third instar larvae do not show significant differences in bouton number at the neuromuscular junction compared to wild type or single heterozygotes.

(Harris et al., 2016)

wit^A12/+ significantly suppresses the increases in mature boutons and branch number seen at the neuromuscular junction of Fmr1^Δ113M/+ third instar larvae (though bouton and branch number is significantly higher in Fmr1^Δ113M/+;wit^A12/+ larvae than wit^A12/+ larvae). wit^A12/wit^B11 fully suppresses the increase in mature boutons and branch number seen at the neuromuscular junction of Fmr1^Δ113M/+ third instar larvae (branch number is significantly higher in wit^A12/wit^B11 larvae than Fmr1^Δ113M/+;wit^A12/wit^B11 larvae).

(Kashima et al., 2016)

Introducing a single copy of wit^A12 into the cpx^SH1 mutant background also rescues the synaptic overgrowth but not the elevated minis, combination with wit^A12/wit^B11 reduces the number of NMJ boutons in cpx^SH1 mutants even further, below the control levels and is comparable to that observed in wit^A12/wit^B11 mutants alone.

(Cho et al., 2015)

One copy of wit^A12 suppresses the increase in total bouton number seen in S6KL¹⁴⁰ mutants.

(Zhao et al., 2015)

wit^A12/wit^B11 Acsl⁰⁵⁸⁴⁷/Acsl^KO double mutants show a significant reduction in bouton number at neuromuscular junction 4, similar to wit^A12/wit^B11 mutants.

wit^A12/+ significantly suppresses synaptic overgrowth of Acsl⁰⁵⁸⁴⁷/Acsl^KO mutants.

(Liu et al., 2014)

wit^A12/+ suppresses the increase in bouton number and satellite bouton number seen at the neuromuscular junction in spartin¹/Df(3R)110 third instar larvae.

wit^A12 spartin¹/wit^B11 Df(3R)110 double mutant larvae show a significant decrease in bouton number and satellite bouton number at the neuromuscular junction compared to controls, as occurs in wit^A12/wit^B11 single mutant larvae.

wit^A12/+ strongly suppresses the brain vacuolisation seen in 20 day old spartin¹/Df(3R)110 adults.

(Nahm et al., 2013)

The neuromuscular junction overgrowth phenotype (increased total number of boutons and increased number of satellite boutons) of brat¹¹/brat^2L-192-9 larvae is not suppressed by wit^A12/+.

(Shi et al., 2013)

Expression of twit^Scer\UAS.cKa under the control of Scer\GAL4^elav.PU slightly, but significantly, enhances the reduction in synaptic terminal size at the neuromuscular junction seen in wit^B11/wit^A12 larvae.

Expression of twit^Scer\UAS.cKa under the control of Scer\GAL4^elav.PU has no effect on the size of the evoked excitatory junctional potential (EJP) at the neuromuscular junction in wit^B11/wit^A12 larvae.

Expression of twit^Scer\UAS.cKa under the control of Scer\GAL4^elav.PU has no effect on miniature EJP (mEJP) amplitude at the neuromuscular junction in wit^B11/wit^A12 larvae, while mEJP frequency is slightly increased.

(Kim and Marqués, 2012)

The neuromuscular junction expansion phenotype seen in cmpy^Δ8 mutants is partially suppressed when animals are also heterozygous for wit^A12.

wit^A12, cmpy^Δ8 double mutants show a 44% reduction in bouton number at neuromuscular junction 4, thereby phenocopying the neuromuscular junction undergrowth displayed by wit single mutants.

(James and Broihier, 2011)

wit^A12/wit^B11 suppresses the increase in average bouton number at the neuromuscular junction which is seen in larvae expressing Snx16^{3A.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^elav-C155.

(Rodal et al., 2011)

Expression of wit^A12/wit^HA3 suppresses the larval neuromuscular junction overgrowth and bouton number phenotypes seen when Rac1^{Scer\UAS.T:Hsap\MYC} is expressed in motor neurons under the control of Scer\GAL4^BG380, instead producing a reduction in bouton number similar to the phenotype seen in wit^A12/wit^HA3 third instar larvae.

Expression of wit^A12/wit^HA3 fails to suppress the larval neuromuscular junction overgrowth and bouton number phenotypes seen when Rac1^V12.Scer\UAS is expressed in motor neurons under the control of Scer\GAL4^BG380.

wit^A12/wit^HA3 suppresses the increase in evoked excitatory junctional potential (EJPs) and quantal content seen when Rac1^Scer\UAS.cLa is expressed in motor neurons under the control of Scer\GAL4^BG380, with third instar larvae instead displaying the reduction in neurotransmitter release phenotype seen in wit^A12/wit^HA3 mutants.

Expression of trio^Scer\UAS.cBa in motor neurons under the control of Scer\GAL4^BG380 partially suppresses the reduced bouton number phenotype seen in wit^A12/wit^HA3 larval neuromuscular junctions.

(Ball et al., 2010)

Ranbp11⁷⁰/+; wit^A12/+ double heterozygous larvae exhibit significantly fewer boutons than either wild-type or each heterozygous allele alone.

(Higashi-Kovtun et al., 2010)

WASp¹/Df(3R)3450 partially suppresses the small neuromuscular junction phenotype seen in wit^A12/wit^B11 mutants.

(Khuong et al., 2010)

RhoGAP92B¹/+ wit^A12/+ double heterozygous third instar larvae show a significant decrease in bouton number/muscle area and satellite bouton number at the neuromuscular junction compared to wild type.

(Nahm et al., 2010)

Expression of Cip4^NIG.15015R or WASp^GD1559 post-synaptically (under the control of Scer\GAL4^C57) in wit^A12/wit^B11 larvae does not affect overall bouton number or satellite bouton formation.

A wit^A12 heterozygous background can completely reverse the increase in total bouton number and satellite bouton formation in larvae expressing either Cip4^NIG.15015R or WASp^GD1559 post-synaptically (under the control of Scer\GAL4^C57).

(Nahm et al., 2010)

Post-synaptic expression of Dys^{dsRNA.mub.Scer\UAS} under the control of Scer\GAL4^eve.RN2 in a wit^A12/wit^B11 background increases the synaptic current as in a wild-type background.

(Fradkin et al., 2008)

wit^A12/+ suppresses the increase in total bouton number at the neuromuscular junction that is seen in nwk¹/nwk² larvae.

The increase in total bouton number at the neuromuscular junction that is seen in Dap160^Δ1 larvae is suppressed by wit^A12/+.

(O'Connor-Giles et al., 2008)

A wit^A12/wit^B11 transheterozygous background suppresses the synaptic overgrowth and increased bouton number phenotypes found in nwk¹ homozygous third instar larvae, instead the larvae exhibit reduced synaptic growth and fewer boutons than wild type controls.

(Rodal et al., 2008)

spict^mut neuromuscular junction overgrowth phenotypes are fully suppressed in wit^A12/wit^B11 mutants. The synaptic undergrowth phenotypes in larvae homozygous for spict^mut in a wit^A12/wit^B11 background are indistinguishable from that of wit^A12/wit^B11 mutants alone. In addition, a heterozygous wit^A12 background partially suppresses the neuromuscular junction expansion of spict^mut larvae.

(Wang et al., 2007)

Expression of Dys^{dsRNA.NH2.Scer\UAS} postsynaptically (under the control of Scer\GAL4^G14) in wit^A12/wit^B11 trans-heterozygous neuromuscular junctions results in similar EJP amplitudes and quantal content as in wit^A12/wit^B11 mutants.

(van der Plas et al., 2006)

At 25^oC ~80% of wit^A12/wit^B11, Fmrf^Scer\UAS.cMa (driven by Scer\GAL4^dimm-929) animals can partially escape the pupal case. At 28^oC about 30% of females fully eclose, and some inflate their wings. While these animals move fairly well they usually die within several days without producing progeny. No rescue is seen in the size of the synapse. Nor is the primary defect in synaptic transmission rescued.

(Marques et al., 2003)

One copy of wit^A12 suppresses the increased bouton number seen at the neuromuscular junction in spin¹⁰⁴⁰³/spin^k09905 animals. spin¹⁰⁴⁰³/spin^k09905 animals which are also mutant for wit^A12/wit^B11 show a further decrease in bouton number.

(Sweeney and Davis, 2002)

Xenogenetic Interactions

Statement

Reference

Heterozygosity for wit^A12 does not modify the fully penetrant rough eye phenotype resulting from the co-expression of HPV18\E6^{Scer\UAS.T:Hsap\MYC} and Hsap\UBE3A^Scer\UAS.cRa under the control of Scer\GAL4^GMR.PU, leading to severe eye necrosis.

(Padash Barmchi et al., 2016)

Complementation and Rescue Data

Rescued by

wit^B11/wit^A12 is rescued by Scer\GAL4^elav-C155/wit^UAS.EGFP

(Smith et al., 2012)

wit^B11/wit^A12 is rescued by Scer\GAL4^elav-C155/wit^UAS.CFP

(Smith et al., 2012)

Partially rescued by

wit^B11/wit^A12 is partially rescued by Scer\GAL4^CCAP.PP/wit^UAS.cMa

(Veverytsa and Allan, 2011)

wit^A12/wit^C175 is partially rescued by Scer\GAL4^elav-C155/wit^UAS.cMa

(Marques et al., 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

5173

bw¹; wit^A12 st¹/TM6B, Tb⁺

Kyoto

108085

bw¹; wit^A12 st¹/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Wit^A12

(Kashima et al., 2017)

l(3)64Aa^A12

(Featherstone et al., 2000, Harrison, 1999.5.12)

wit^A12

(Klaustermeier et al., 2025, Kaneko et al., 2024, Kang et al., 2024, Rubio-Ferrera et al., 2023, Zhou et al., 2023, Chen et al., 2022, Park et al., 2022, Peterson et al., 2022, Vuilleumier et al., 2022, Berndt et al., 2020, Kamimura et al., 2019, Kim et al., 2019, Politano et al., 2019, Vuilleumier et al., 2019, Wang et al., 2019, Hoyer et al., 2018, Liao et al., 2018, Akbergenova and Littleton, 2017, Banerjee et al., 2017, Deshpande et al., 2016, Harris et al., 2016, Kashima et al., 2016, Padash Barmchi et al., 2016, Sulkowski et al., 2016, Berndt et al., 2015, Bivik et al., 2015, Cho et al., 2015, Zhao et al., 2015, Liu et al., 2014, Sulkowski et al., 2014, Nahm et al., 2013, Shi et al., 2013, Kim and Marqués, 2012, Smith et al., 2012, James and Broihier, 2011, Pilgram et al., 2011, Veverytsa and Allan, 2011, Ball et al., 2010, Higashi-Kovtun et al., 2010, Khuong et al., 2010, Nahm et al., 2010, Nahm et al., 2010, Chang et al., 2008, Fradkin et al., 2008, Haussmann et al., 2008, Miguel-Aliaga et al., 2008, O'Connor-Giles et al., 2008, Rodal et al., 2008, Baumgardt et al., 2007, Christensen et al., 2007.10.29, Herrero et al., 2007, Korolchuk et al., 2007, Wang et al., 2007, Collins et al., 2006, van der Plas et al., 2006)

wit^a12

(Rodal et al., 2011)

witA12

(Zhang et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (68)

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