FlyBase Allele Report: Scer\GAL4[da.Switch.PT]

General Information

Symbol

Scer\GAL4^da.Switch.PT

Species

S. cerevisiae

Name

FlyBase ID

FBal0249022

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{da-GSGAL4.T}

Also Known As

daGS, da-GS, da-GS-Gal4, DaGS-Gal4, daughterless-GeneSwitch, da-GSG, daughterless-Gene-Switch, Da-GeneSwitch, daughterlessGeneSwitch

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Tricoire et al., 2009)

Progenitor genotype

Carried in construct

P{da-GSGAL4.T}

Cytology

Description

A 3178bp genomic fragment from immediately 5' of the da ATG start codon is fused upstream of the ligand-inducible GAL4::GS driver.

(Tricoire et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

Encoded product / tool

GAL4::GS

binary expression system - small molecule-regulated driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage -- adult stage

organism | ubiquitous

(Paik et al., 2012, Tricoire et al., 2009)

Additional Information

Statement

Reference

Drives mifepristone-inducible expression ubiquitously.

(FlyBase Curators, 2017)

ScerGAL4^da.Switch.PT drives mifepristone-inducible expression ubiquitously.

(Paik et al., 2012)

ScerGAL4^da.Switch.PT drives mifepristone-inducible expression in a broad pattern; in particular, drives expression in all tissues known to influence lifespan including gut, fat body, muscles, brain and gonads. Expression is stable throughout age in females, and increases in males.

(Tricoire et al., 2009)

Marker for

Reflects expression of

(Paik et al., 2012, Tricoire et al., 2009)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | RU486 conditional, with ATPsynO^GD4768

(Rera et al., 2010)

abnormal developmental rate | RU486 conditional, with Coq7^GD8325

(Rera et al., 2010)

abnormal developmental rate | RU486 conditional, with COX5B^GD6659

(Rera et al., 2010)

abnormal developmental rate | RU486 conditional, with Cyt-c1^GD3851

(Rera et al., 2010)

abnormal developmental rate | RU486 conditional, with Lon^GD14030

(Rera et al., 2010)

abnormal developmental rate | RU486 conditional, with mRpL50^GD4975

(Rera et al., 2010)

abnormal developmental rate | RU486 conditional, with ND-51^GD5335

(Rera et al., 2010)

abnormal feeding behavior | adult stage | RU486 conditional, with ITP^UAS.cHa

(Gáliková et al., 2018)

abnormal feeding behavior | adult stage | RU486 conditional, with ITP^VSH330029

(Gáliková et al., 2018)

abnormal feeding behavior | adult stage | RU486 conditional, with Prosβ5^UAS.cNa

(Nguyen et al., 2019)

abnormal feeding behavior | adult stage | RU486 conditional, with Stim^RNAi.cEa.UAS

(Xu et al., 2019)

abnormal immune response | adult stage | RU486 conditional, with imd^CA.UAS

(Yamashita et al., 2021)

abnormal locomotor behavior | female limited, with park^UAS.cGa

(Rana et al., 2013)

abnormal osmotic stress response | adult stage | RU486 conditional, with ITP^UAS.cHa

(Gáliková et al., 2018)

abnormal osmotic stress response | adult stage | RU486 conditional, with ITP^VSH330029

(Gáliková et al., 2018)

abnormal oxidative stress response | adult stage | :chemical conditional | RU486 conditional, with fh^200GAA, fh^UAS.cAa

(Russi et al., 2020)

abnormal oxidative stress response | female limited | RU486 conditional, with ATPsynD^KK107800

(Sun et al., 2014)

abnormal starvation stress response, with park^UAS.cGa

(Rana et al., 2013)

abnormal starvation stress response | adult stage | RU486 conditional, with imd^CA.UAS

(Yamashita et al., 2021)

abnormal starvation stress response | adult stage | RU486 conditional, with Prosβ5^UAS.cNa

(Nguyen et al., 2019)

abnormal starvation stress response | female limited | RU486 conditional, with ATPsynD^KK107800

(Sun et al., 2014)

abnormal starvation stress response | RU486 conditional, with schlank^JF02502

(Nelson et al., 2016)

abnormal stress response | adult stage | RU486 conditional, with imd^CA.UAS

(Yamashita et al., 2021)

abnormal stress response | adult stage | RU486 conditional, with Nmdmc^UAS.cYa

(Yu et al., 2015)

chemical resistant | RU486 conditional, with Pi3K92E^{D954A.UAS.Tag:MYC}

(Slack et al., 2011)

chemical sensitive | adult stage | RU486 conditional, with fh^200GAA, fh^UAS.cAa

(Russi et al., 2020)

decreased body weight | adult stage | RU486 conditional, with imd^CA.UAS

(Yamashita et al., 2021)

decreased fecundity | female | RU486 conditional, with EcR^RNAi.UAS.cCa

(Tricoire et al., 2009)

decreased fecundity | female | RU486 conditional, with Pi3K92E^{D954A.UAS.Tag:MYC}

(Slack et al., 2011)

decreased rate of feeding behavior | adult stage | RU486 conditional, with imd^CA.UAS

(Yamashita et al., 2021)

female sterile | RU486 conditional, with ATPsynO^GD4768

(Rera et al., 2010)

female sterile | RU486 conditional, with ATPsynβ^GD4967

(Rera et al., 2010)

female sterile | RU486 conditional, with COX5B^GD6659

(Rera et al., 2010)

increased fecundity | RU486 conditional, with park^UAS.cGa

(Rana et al., 2013)

increased mortality | adult stage | :chemical conditional | RU486 conditional, with fh^200GAA, fh^UAS.cAa

(Russi et al., 2020)

increased mortality | adult stage | :conditional | RU486 conditional, with ITP^UAS.cHa

(Gáliková et al., 2018)

increased mortality | adult stage | :conditional | RU486 conditional, with ITP^VSH330029

(Gáliková et al., 2018)

increased mortality | adult stage | :RU486 conditional | conditional, with imd^CA.UAS

(Yamashita et al., 2021)

lethal, with CG10209^GX193

(Paik et al., 2012)

lethal, with cv-2^GX6589

(Paik et al., 2012)

lethal, with dan^GX7554

(Paik et al., 2012)

lethal, with PGRP-LC^GX6548

(Paik et al., 2012)

lethal, with RapGAP1^GX5503-1

(Paik et al., 2012)

lethal | RU486 conditional, with ATPsynO^GD4768

(Rera et al., 2010)

lethal | RU486 conditional, with COX5B^GD6659

(Rera et al., 2010)

lethal | RU486 conditional, with ND-51^GD5335

(Rera et al., 2010)

long lived, with Agbe^GX8331

(Paik et al., 2012)

long lived, with Agbe^UAS.cPa

(Paik et al., 2012)

long lived, with CalpA^GX16168

(Paik et al., 2012)

long lived, with CG8155^EP2612

(Paik et al., 2012)

long lived, with CG10383^GX47642-1

(Paik et al., 2012)

long lived, with CG10916^GX47642

(Paik et al., 2012)

long lived, with CG30427^EP22086

(Paik et al., 2012)

long lived, with CG42663^GX2970-1

(Paik et al., 2012)

long lived, with Dlc90F^GX48290

(Paik et al., 2012)

long lived, with ImpL2^s.UAS

(Paik et al., 2012)

long lived, with InR^K1409A.UAS

(Slack et al., 2011)

long lived, with kis^GX2970

(Paik et al., 2012)

long lived, with kis^GX6561

(Paik et al., 2012)

long lived, with Men^EP1250

(Paik et al., 2012)

long lived, with PGRP-LF^GX5503

(Paik et al., 2012)

long lived, with sfl^circ-ORF.UAS

(Weigelt et al., 2020)

long lived, with SIFaR^GX47280-1

(Paik et al., 2012)

long lived, with Sin3A^GX8304

(Paik et al., 2012)

long lived, with sm^GX21702-1

(Paik et al., 2012)

long lived | female | RU486 conditional, with Adf1^GD1358

(Zane et al., 2023)

long lived | female | RU486 conditional, with lncRNA:let7C^UAS.cSa

(Gendron and Pletcher, 2017)

long lived | female | RU486 conditional, with Prp19^UAS.cGa

(Garschall et al., 2017)

long lived | female | RU486 conditional, with Sag1^HMC03384

(Zane et al., 2023)

long lived | female | RU486 conditional, with Trl^GL00699

(Zane et al., 2023)

long lived | female limited, with park^UAS.cGa

(Rana et al., 2013)

long lived | female limited | :RU486 conditional | nutrition conditional, with ATPsynD^KK107800

(Sun et al., 2014)

long lived | RU486 conditional, with daw^HMS01110

(Bai et al., 2013)

long lived | RU486 conditional, with EcR^{B1-ΔC655.F645A.UAS}

(Tricoire et al., 2009)

long lived | RU486 conditional, with EcR^{B1-ΔC655.W650A.UAS}

(Tricoire et al., 2009)

long lived | RU486 conditional, with gig^UAS.cTa

(Sun et al., 2014)

long lived | RU486 conditional, with Glyp^S15D.UAS

(Post et al., 2018)

long lived | RU486 conditional, with Glyp^UAS.cPa

(Post et al., 2018)

long lived | RU486 conditional, with InR^K1409A.UAS

(Slack et al., 2011)

long lived | RU486 conditional, with Nmdmc^UAS.cYa

(Yu et al., 2015)

long lived | RU486 conditional, with Pi3K92E^{D954A.UAS.Tag:MYC}

(Slack et al., 2011)

long lived | RU486 conditional, with Prosβ5^UAS.cNa

(Nguyen et al., 2019)

long lived | RU486 conditional, with Ras85D^HMS01294

(Slack et al., 2015)

long lived | RU486 conditional, with Ras85D^N17.UAS

(Slack et al., 2015)

long lived | RU486 conditional, with rl^HMS00173

(Slack et al., 2015)

partially lethal - majority die | RU486 conditional, with imd^CA.UAS

(Yamashita et al., 2021)

short lived, with Aef1^HMC06626

(Zane et al., 2023)

short lived, with dmrt93B^{UAS.ORF.GW.Tag:HA}

(Zane et al., 2023)

short lived, with Ets21C^HMS01989

(Zane et al., 2023)

short lived, with Ets21C^{UAS.ORF.GW.Tag:HA}

(Zane et al., 2023)

short lived, with Ets96B^{UAS.ORF.GW.Tag:HA}

(Zane et al., 2023)

short lived, with GATAd^HMS01300

(Zane et al., 2023)

short lived, with GATAe^HMS01087

(Zane et al., 2023)

short lived, with Hsf^GL00698

(Zane et al., 2023)

short lived, with ImpL2^GX4499

(Paik et al., 2012)

short lived, with kay^JF02804

(Zane et al., 2023)

short lived, with Mef2^JF03115

(Zane et al., 2023)

short lived, with Nf-YB^HMC04645

(Zane et al., 2023)

short lived, with srp^{UAS.ORF.GW.Tag:HA}

(Zane et al., 2023)

short lived | female | RU486 conditional, with ATPsynO^GD4768

(Rera et al., 2010)

short lived | female | RU486 conditional, with ATPsynβ^GD4967

(Rera et al., 2010)

short lived | female | RU486 conditional, with Coq7^GD8325

(Rera et al., 2010)

short lived | female | RU486 conditional, with COX5B^GD6659

(Rera et al., 2010)

short lived | female | RU486 conditional, with EcR^{B1-ΔC655.F645A.UAS}

(Tricoire et al., 2009)

short lived | female | RU486 conditional, with EcR^{B1-ΔC655.W650A.UAS}

(Tricoire et al., 2009)

short lived | female | RU486 conditional, with EcR^RNAi.UAS.cCa

(Tricoire et al., 2009)

short lived | female | RU486 conditional, with Lon^GD14030

(Rera et al., 2010)

short lived | female | RU486 conditional, with mRpL50^GD4975

(Rera et al., 2010)

short lived | male | RU486 conditional, with ATPsynO^GD4768

(Rera et al., 2010)

short lived | male | RU486 conditional, with COX5B^GD6659

(Rera et al., 2010)

short lived | male | RU486 conditional, with Lon^GD14030

(Rera et al., 2010)

short lived | male | RU486 conditional, with mRpL50^GD4975

(Rera et al., 2010)

short lived | RU486 conditional, with aop^HMS01256

(Slack et al., 2015)

short lived | RU486 conditional, with dpp^HMS00011

(Bai et al., 2013)

short lived | RU486 conditional, with EcR^{B1-ΔC655.F645A.UAS}

(Tricoire et al., 2009)

short lived | RU486 conditional, with EcR^{B1-ΔC655.W650A.UAS}

(Tricoire et al., 2009)

short lived | RU486 conditional, with imd^CA.UAS

(Yamashita et al., 2021)

short lived | RU486 conditional, with Nmdmc^RNAi.UAS.cUa

(Yu et al., 2015)

short lived | RU486 conditional, with pnt^P1.UAS

(Slack et al., 2015)

short lived | RU486 conditional, with Ras85D^V12.cUa.UAS

(Slack et al., 2015)

short lived | RU486 conditional, with Rel^UAS.cUa

(Yu et al., 2015)

short lived | RU486 conditional, with sfl^HMS00543

(Weigelt et al., 2020)

short lived | RU486 conditional, with Tnpo^HMJ23009

(Goodman et al., 2021)

viable | RU486 conditional, with Pi3K92E^{D954A.UAS.Tag:MYC}

(Slack et al., 2011)

Phenotype Manifest In

mitochondrion | female limited, with park^UAS.cGa

(Rana et al., 2013)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^da.Switch.PT, gig^UAS.cTa has long lived | RU486 conditional phenotype, non-enhanceable by ATPsynD^KK107800, Scer\GAL4^da.Switch.PT

(Sun et al., 2014)

Suppressed by

Statement

Reference

Scer\GAL4^da.Switch.PT, Stim^RNAi.cEa.UAS has abnormal feeding behavior | adult stage | RU486 conditional phenotype, suppressible by Akh^A/Akh^A

(Xu et al., 2019)

InR^K1409A.UAS, Scer\GAL4^da.Switch.PT has long lived phenotype, suppressible | partially by foxo^Δ94

(Slack et al., 2011)

InR^K1409A.UAS, Scer\GAL4^da.Switch.PT has long lived | RU486 conditional phenotype, suppressible by foxo^Δ94

(Slack et al., 2011)

Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^da.Switch.PT has long lived | RU486 conditional phenotype, suppressible by foxo^Δ94

(Slack et al., 2011)

Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^da.Switch.PT has long lived phenotype, suppressible | partially by Scer\GAL4^da.Switch.PT

(Slack et al., 2011)

NOT suppressed by

Statement

Reference

Ras85D^V12.cUa.UAS, Scer\GAL4^da.Switch.PT has short lived | RU486 conditional phenotype, non-suppressible by chico¹/chico[+]

(Slack et al., 2015)

Scer\GAL4^da.Switch.PT, aop^HMS01256 has short lived | RU486 conditional phenotype, non-suppressible by chico¹/chico[+]

(Slack et al., 2015)

Scer\GAL4^da.Switch.PT, pnt^P1.UAS has short lived | RU486 conditional phenotype, non-suppressible by chico¹/chico[+]

(Slack et al., 2015)

Scer\GAL4^da.Switch.PT, gig^UAS.cTa has long lived | RU486 conditional phenotype, non-suppressible by ATPsynD^KK107800, Scer\GAL4^da.Switch.PT

(Sun et al., 2014)

Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^da.Switch.PT has viable | RU486 conditional phenotype, non-suppressible by foxo^Δ94

(Slack et al., 2011)

Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^da.Switch.PT has decreased fecundity | female | RU486 conditional phenotype, non-suppressible by foxo^Δ94

(Slack et al., 2011)

NOT Enhancer of

Statement

Reference

ATPsynD^KK107800, Scer\GAL4^da.Switch.PT is a non-enhancer of long lived | RU486 conditional phenotype of Scer\GAL4^da.Switch.PT, gig^UAS.cTa

(Sun et al., 2014)

Suppressor of

Statement

Reference

Tspo^UAS.cRa/Scer\GAL4^da.Switch.PT is a suppressor | partially of short lived phenotype of fh^GAAs

(Jullian et al., 2024)

Hsap\FXN^UAS.cJa/Scer\GAL4^da.Switch.PT is a suppressor of short lived phenotype of fh^GAAs

(Jullian et al., 2024)

Ras85D^V12.cUa.UAS/Scer\GAL4^da.Switch.PT is a suppressor of long lived | dominant phenotype of chico¹

(Slack et al., 2015)

Scer\GAL4^da.Switch.PT is a suppressor | partially of long lived phenotype of Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^da.Switch.PT

(Slack et al., 2011)

NOT Suppressor of

Statement

Reference

Scer\GAL4^da.Switch.PT/Tspo^KK102775 is a non-suppressor of lethal | recessive phenotype of fh^200GAA

(Russi et al., 2020)

Scer\GAL4^da.Switch.PT/Tspo^GD788 is a non-suppressor of lethal | recessive phenotype of fh^200GAA

(Russi et al., 2020)

Tspo^UAS.cRa/Scer\GAL4^da.Switch.PT is a non-suppressor of lethal | recessive phenotype of fh^200GAA

(Russi et al., 2020)

ATPsynD^KK107800, Scer\GAL4^da.Switch.PT is a non-suppressor of long lived | RU486 conditional phenotype of Scer\GAL4^da.Switch.PT, gig^UAS.cTa

(Sun et al., 2014)

InR^K1409A.UAS/Scer\GAL4^da.Switch.PT is a non-suppressor of viable | RU486 conditional phenotype of foxo^Δ94

(Slack et al., 2011)

Pi3K92E^{D954A.UAS.Tag:MYC}/Scer\GAL4^da.Switch.PT is a non-suppressor of viable | RU486 conditional phenotype of foxo^Δ94

(Slack et al., 2011)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^da.Switch.PT/fh^UAS.cJa rescues fh^GAAs

(Jullian et al., 2024)

fh^UAS.cAa/Scer\GAL4^da.Switch.PT rescues fh^18GAA

(Russi et al., 2020)

fh^UAS.cAa/Scer\GAL4^da.Switch.PT rescues fh^200GAA

(Russi et al., 2020)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^da.Switch.PT

Name Synonyms

Secondary FlyBase IDs

References (84)

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