FlyBase Gene Report: Dmel\γTub37C

General Information

Symbol

Dmel\γTub37C

Species

D. melanogaster

Name

γ-Tubulin at 37C

Annotation Symbol

CG17566

Feature Type

protein_coding_gene

FlyBase ID

FBgn0010097

Gene Model Status

Current

Stock Availability

17 publicly available

Gene Summary

γ-Tubulin at 37C (γTub37C) encodes a maternally expressed member of the tubulin family. It localizes predominantly to centrosomes and plays an essential role in microtubule nucleation at the centrosomes. [Date last reviewed: 2019-03-21] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

γ-tubulin, γ tubulin, γtubulin, γ-Tub, gamma-tubulin

Key Links

Genomic Location

Cytogenetic map

37C7-37C7

Sequence location

2L:19,183,863..19,185,726 [-]

Recombination map

2-54

RefSeq locus

NT_033779 REGION:19183863..19185726

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (21 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables GTP binding

inferred from direct assay

(Gunawardane et al., 2003)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables GTP binding

inferred from biological aspect of ancestor with PANTHER:PTN000172598

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR003008, InterPro:IPR017975

(InterPro Project Members, 2004-)

enables microtubule nucleator activity

inferred from biological aspect of ancestor with PANTHER:PTN000172659

(GO Reference Genome Project, 2011-)

Biological Process (11 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in attachment of meiotic spindle microtubules to kinetochore

inferred from mutant phenotype

(Hughes et al., 2011)

involved_in mitotic spindle-templated microtubule nucleation

inferred from direct assay

(Tariq et al., 2020)

involved_in oocyte nucleus migration involved in oocyte dorsal/ventral axis specification

inferred from mutant phenotype

(Hughes et al., 2011)

involved_in spindle assembly involved in female meiosis I

inferred from mutant phenotype

(Hughes et al., 2011)

Terms Based on Predictions or Assertions (7 terms)

CV Term

Evidence

References

involved_in cytoplasmic microtubule organization

inferred from electronic annotation with InterPro:IPR002454

(InterPro Project Members, 2004-)

involved_in meiotic spindle organization

inferred from biological aspect of ancestor with PANTHER:PTN000172659

(GO Reference Genome Project, 2011-)

involved_in microtubule nucleation

inferred from electronic annotation with InterPro:IPR002454

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000172659

(GO Reference Genome Project, 2011-)

involved_in microtubule-based process

inferred from electronic annotation with InterPro:IPR000217, InterPro:IPR017975

(InterPro Project Members, 2004-)

involved_in mitotic cell cycle

inferred from biological aspect of ancestor with PANTHER:PTN000172598

(GO Reference Genome Project, 2011-)

involved_in mitotic sister chromatid segregation

inferred from biological aspect of ancestor with PANTHER:PTN000172659

(GO Reference Genome Project, 2011-)

involved_in mitotic spindle organization

inferred from biological aspect of ancestor with PANTHER:PTN000172659

(GO Reference Genome Project, 2011-)

Cellular Component (8 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

located_in centrosome

inferred from direct assay

(Hallen et al., 2008)

located_in cytoplasm

inferred from direct assay

(Hallen et al., 2008)

part_of gamma-tubulin ring complex

inferred from direct assay

(Tariq et al., 2020)

located_in meiotic spindle

inferred from direct assay

(Hughes et al., 2011)

located_in spindle

inferred from direct assay

(Hallen et al., 2008)

Terms Based on Predictions or Assertions (7 terms)

CV Term

Evidence

References

is_active_in centrosome

inferred from biological aspect of ancestor with PANTHER:PTN000172659

(GO Reference Genome Project, 2011-)

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000172598

(GO Reference Genome Project, 2011-)

part_of gamma-tubulin complex

inferred from electronic annotation with InterPro:IPR002454

(InterPro Project Members, 2004-)

part_of gamma-tubulin ring complex

inferred from biological aspect of ancestor with PANTHER:PTN000172659

(GO Reference Genome Project, 2011-)

located_in microtubule

inferred from electronic annotation with InterPro:IPR000217, InterPro:IPR017975

(InterPro Project Members, 2004-)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000172659

(GO Reference Genome Project, 2011-)

is_active_in spindle

inferred from biological aspect of ancestor with PANTHER:PTN000172659

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

GAMMA-TUBULIN RING COMPLEX

GAMMA-TUBULIN SMALL COMPLEX

GAMMA TUBULINS

Protein Family (UniProt)

Belongs to the tubulin family. (P42271)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

GAMMA-TUBULIN RING COMPLEX -: The gamma-tubulin ring complex (γ-TuRC) is a multiprotein complex composed of the γ-tubulin small complex (γ-TuSC) plus additional Grip-motif proteins to form the ring-shaped complex. γ-TuRC localizes to the centrosome and catalyze the microtubule nucleation. (Adapted from FBrf0190946 and FBrf0259816.)
GAMMA-TUBULIN SMALL COMPLEX -: The gamma-tubulin small complex (γ-TuSC) forms the core of the gamma-tubulin ring complex (γ-TuRC). It is a heterotetramer composed of two molecules of γ-tubulin associated with two Grip-motif proteins. γ-TuSC acts as a microtubule nucleator. (Adapted from FBrf0191157).
GAMMA TUBULINS -: Tubulins are cytoskeletal proteins. γ- tubulin is principally involved in the nucleation of microtubules at microtubule organising centres. (Adapted from PMID:1121746).

Protein Function (UniProtKB)

Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome, suggesting that it is involved in the minus-end nucleation of microtubule assembly. Required for oocyte activation and consequently for organization of the female meiotic spindle (PubMed:9155007, PubMed:9698447). Essential for centrosome organization and assembly of biastral mitotic spindles in embryos. Plays a role in stabilizing the augmin complex on the meiotic spindle (PubMed:23785300).

(UniProt, P42271)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

fs(2)TW1: female sterile (2) Ted Wright 1

Eggs produced by homozygous females show no visible signs of development (Schupbach, and Wieschaus, 1989, Genetics 121: 101-17).

Summary (Interactive Fly)

microtubule nucleating factor - eggs produced by mutant mothers show an arrest of nuclear divisions during early embryogenesis and a disruption in the anterior-posterior axis - required for bicoid mRNA localization

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\γTub37C for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P42271)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.45

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

γTub37C-RA

FBtr0081228

NM_057574

1521

457

γTub37C-RB

FBtr0332572

NM_001273636

1628

457

Additional Transcript Data and Comments

Reported size (kB)

1.553 (unknown)

(Wilson et al., 1997)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

γTub37C-PA

FBpp0080769

51.3

457

6.25

FBpp0304827

51.3

457

6.25

P42271

NP_001260565

AGB93100

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

457 aa isoforms: γTub37C-PA, γTub37C-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with Ote.

(UniProt, P42271)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\γTub37C using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

gammaTub37C_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.30

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

cell

• centrosome

(Vidwans et al., 1999)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in centrosome

inferred from direct assay

(Hallen et al., 2008)

located_in cytoplasm

inferred from direct assay

(Hallen et al., 2008)

part_of gamma-tubulin ring complex

inferred from direct assay

(Tariq et al., 2020)

located_in meiotic spindle

inferred from direct assay

(Hughes et al., 2011)

located_in spindle

inferred from direct assay

(Hallen et al., 2008)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\γTub37C in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\γTub37C

View exonic expression by tissue for Dmel\γTub37C

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0010097

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35199/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0010097

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0010097

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0010097

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG17566

Flygut - An atlas of the Drosophila adult midgut

FBgn0010097

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 24 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 12 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of γTub37C

Transgenic constructs containing regulatory region of γTub37C

Aberrations (Deficiencies and Duplications) ( 28 )

Inferred from experimentation ( 28 )

Gene disrupted in

Df(2L)Exel6043

(Ryder, 2004.4.26)

Df(2L)NST

(Wright, 1996, Stathakis et al., 1995)

Df(2L)Sd57

(Wright, 1996, Stathakis et al., 1995)

Df(2L)Sd77

(Wright, 1996, Stathakis et al., 1995)

Df(2L)TE37C-1

(Wright, 1996)

Df(2L)TE37C-7

(Wright, 1996, Stathakis et al., 1995)

Df(2L)TW50

(Schupbach and Wieschaus, 1991, Schupbach and Wieschaus, 1989)

Df(2L)TW130

(Schnorrer et al., 2002, Wright, 1996, Stathakis et al., 1995, Schupbach and Wieschaus, 1991, Schupbach and Wieschaus, 1989)

Df(2L)TW150

(Schnorrer et al., 2002)

Df(2L)TW158

(Schnorrer et al., 2002, Tavosanis et al., 1997)

Df(2L)VA12

(Wright, 1996, Stathakis et al., 1995)

Df(2L)VA13

(Wright, 1996)

Df(2L)VA16

(Schnorrer et al., 2002)

Df(2L)VA17

(Wright, 1996, Stathakis et al., 1995)

Df(2L)VA19

(Tavosanis et al., 1997, Wright, 1996, Stathakis et al., 1995)

Df(2L)VA23

(Hallen et al., 2008, Tavosanis et al., 1997, Stathakis et al., 1995)

Gene not disrupted in

Inferred from location ( 10 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die during embryonic stage | non-rescuable maternal effect | recessive

γTub37C^unspecified

lethal - all die during embryonic stage | recessive

γTub37C¹

viable

γTub37C¹³⁹

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

γTub37C^GD9416

viable, with Scer\GAL4^Mef2.PR

γTub37C^GD9416

viable, with Scer\GAL4^pnr-MD237

γTub37C^GD9416

viable, with Scer\GAL4^tin.CΔ4

γTub37C^GD9416

Sterility

Allele

female sterile

female sterile (with Df(2L)VA23)

γTub37C¹¹

female sterile | recessive

male fertile

Other Phenotypes

Allele

abnormal meiotic cell cycle

γTub37C^P162L

abnormal meiotic cell cycle (with Df(2L)Exel6043)

γTub37C³

abnormal mitotic cell cycle | maternal effect

γTub37C^unspecified

some die during embryonic stage | recessive

wild-type

γTub37C^+t7.2

Phenotype manifest in

Allele

aster | embryonic stage

γTub37C¹

centrosome | maternal effect (with Df(2L)TE37C-7)

condensed nuclear chromosome

γTub37C^P162L

condensed nuclear chromosome (with Df(2L)Exel6043)

γTub37C³

egg & spindle (with Df(2L)TE37C-7)

γTub37C³

embryo & mitosis & nuclear chromosome

meiotic metaphase I

meiotic spindle

γTub37C^P162L

meiotic spindle (with Df(2L)Exel6043)

γTub37C³

nucleus

γTub37C³

nucleus (with γTub37C¹)

γTub37C³

nucleus (with γTub37C³)

γTub37C¹

oocyte

γTub37C³

oocyte (with Df(2L)TE37C-7)

γTub37C³

oocyte (with γTub37C¹)

γTub37C³

oocyte (with γTub37C³)

γTub37C¹

oocyte nucleus

γTub37C^P162L

oocyte nucleus (with Df(2L)Exel6043)

γTub37C³

ovariole | germline clone

γTub37C¹³⁹

polar body nucleus

polar body nucleus (with Df(2L)TE37C-7)

γTub37C³

polar body nucleus (with γTub37C¹)

γTub37C³

polar body nucleus (with γTub37C³)

γTub37C¹

polar body nucleus | maternal effect (with Df(2L)TE37C-7)

γTub37C¹²

spindle

spindle & oocyte (with γTub37C¹)

γTub37C³

spindle & oocyte (with γTub37C³)

γTub37C¹

spindle | cleavage stage | maternal effect

γTub37C^unspecified

spindle | embryonic stage

spindle | maternal effect (with Df(2L)TE37C-7)

γTub37C¹²

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (23)

Hsap\TUBG1

13 of 14

Yes

12 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Hsap\TUBE1

2 of 14

Yes

Hsap\TUBAL3

1 of 14

Hsap\TUBB8B

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (17)

Rnor\Tubg1

13 of 14

Yes

12 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Tube1

1 of 14

Mus musculus (laboratory mouse) (20)

Mmus\Tubg1

13 of 14

Yes

11 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (16)

Xtro\tubg1

8 of 13

Yes

Xtro\entpd3

1 of 13

Yes

Xtro\epc2

1 of 13

Xtro\LOC101732615

1 of 13

Yes

Xtro\selenos

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\tubz1

1 of 13

Yes

Xtro\yipf2

1 of 13

Danio rerio (Zebrafish) (23)

Drer\tubg1

13 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Drer\zgc:55461

2 of 14

Drer\zgc:65894

2 of 14

Drer\si:ch211-114n24.6

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (17)

Cele\tbg-1

13 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (12)

Agam\AgaP_AGAP007834

8 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (17)

Atha\GCP2

13 of 13

Yes

Atha\TUBG1

13 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Saccharomyces cerevisiae (Brewer's yeast) (4)

Scer\TUB4

12 of 13

Yes

Scer\TUB2

2 of 13

Scer\TUB1

1 of 13

Scer\TUB3

1 of 13

Schizosaccharomyces pombe (Fission yeast) (4)

Spom\gtb1

12 of 12

Yes

Spom\nda3

2 of 12

Spom\atb2

1 of 12

Spom\nda2

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:γTub37C. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (11)

12 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

3 of 13

3 of 13

2 of 13

2 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

TUBG1; tubulin gamma 1

model of complex cortical dysplasia with other brain malformations 4

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TUBG1; tubulin gamma 1

13 of 14

191135

CORTICAL DYSPLASIA, COMPLEX, WITH OTHER BRAIN MALFORMATIONS 4; CDCBM4

complex cortical dysplasia with other brain malformations 4

TUBG2; tubulin gamma 2

12 of 14

605785

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

γTub37C - CG8475

experimental knowledge based

(Guruharsha et al., 2011)

γTub37C - Cnb

anti tag coimmunoprecipitation, peptide massfingerprinting, western blot

(Januschke et al., 2013)

γTub37C - Cp190

anti bait coimmunoprecipitation, western blot

(Raff et al., 1993)

γTub37C - Faa

experimental knowledge based

(Guruharsha et al., 2011)

γTub37C - Grip128

anti bait coimmunoprecipitation, molecular weight estimation by staining, cosedimentation through density gradient, western blot, anti tag coimmunoprecipitation

(Tariq et al., 2020, Gopalakrishnan et al., 2012, Wiese, 2008, Gunawardane et al., 2003, Oegema et al., 1999)

γTub37C - Grip163

anti bait coimmunoprecipitation, molecular weight estimation by staining, cosedimentation through density gradient, western blot, anti tag coimmunoprecipitation

(Tariq et al., 2020, Gopalakrishnan et al., 2012, Wiese, 2008, Gunawardane et al., 2003, Oegema et al., 1999)

γTub37C - Grip71

cosedimentation through density gradient, western blot, anti bait coimmunoprecipitation, molecular weight estimation by staining, anti tag coimmunoprecipitation

(Tariq et al., 2020, Goshima et al., 2008, Gunawardane et al., 2003)

γTub37C - Grip75

anti tag coimmunoprecipitation, molecular weight estimation by staining, anti bait coimmunoprecipitation, western blot, cosedimentation through density gradient

(Tariq et al., 2020, Gopalakrishnan et al., 2012, Schnorrer et al., 2002, Oegema et al., 1999)

γTub37C - Grip84

cosedimentation through density gradient, western blot, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation, molecular weight estimation by staining

(Tariq et al., 2020, Gopalakrishnan et al., 2012, Wiese, 2008, Kawaguchi and Zheng, 2004, Gunawardane et al., 2003, Oegema et al., 1999)

γTub37C - Grip91

cosedimentation through density gradient, western blot, anti bait coimmunoprecipitation, molecular weight estimation by staining, anti tag coimmunoprecipitation

(Tariq et al., 2020, Gopalakrishnan et al., 2012, Wiese, 2008, Kawaguchi and Zheng, 2004, Gunawardane et al., 2003, Oegema et al., 1999)

γTub37C - HeT-A\gag

anti tag coimmunoprecipitation, western blot, pull down, Identification by mass spectrometry

(Morgunova et al., 2021)

γTub37C - Iris

experimental knowledge based

(Guruharsha et al., 2011)

γTub37C - Map60

anti bait coimmunoprecipitation, western blot

(Raff et al., 1993)

γTub37C - Mzt1

cosedimentation, western blot, anti tag coimmunoprecipitation

(Tovey et al., 2018)

γTub37C - Nin

pull down, western blot

(Zheng et al., 2016)

γTub37C - Ote

anti tag coimmunoprecipitation, western blot

(Habermann et al., 2012)

γTub37C - PhKγ

experimental knowledge based

(Guruharsha et al., 2011)

γTub37C - Phka

experimental knowledge based

(Guruharsha et al., 2011)

γTub37C - Plp

anti bait coimmunoprecipitation, western blot, molecular sieving, ion exchange chromatography

(Kawaguchi and Zheng, 2004)

γTub37C - Sas-4

anti bait coimmunoprecipitation, western blot, pull down, anti tag coimmunoprecipitation, cosedimentation through density gradient

(Conduit et al., 2015, Zheng et al., 2014, Gopalakrishnan et al., 2012, Gopalakrishnan et al., 2011)

γTub37C - bru3

experimental knowledge based

(Guruharsha et al., 2011)

γTub37C - cnn

pull down, western blot, Identification by mass spectrometry, anti bait coimmunoprecipitation, colocalization, fluorescence microscopy, inferred by author

(Tovey et al., 2021, Habermann et al., 2012, Terada et al., 2003)

γTub37C - coro

experimental knowledge based

(Guruharsha et al., 2011)

γTub37C - dgt6

anti bait coimmunoprecipitation, western blot

(Bucciarelli et al., 2009)

γTub37C - mRpL1

experimental knowledge based

(Guruharsha et al., 2011)

γTub37C - mRpL23

experimental knowledge based

(Guruharsha et al., 2011)

γTub37C - mael

anti bait coimmunoprecipitation, western blot

(Sato et al., 2011)

γTub37C - mv

anti tag coimmunoprecipitation, western blot, Identification by mass spectrometry

(Lattao et al., 2021)

γTub37C - sub

anti tag coimmunoprecipitation, western blot

(Romé and Ohkura, 2018)

γTub37C - swa

anti bait coimmunoprecipitation, western blot

(Schnorrer et al., 2002)

γTub37C - γTub23C

anti bait coimmunoprecipitation, western blot

(Wiese, 2008)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

γTub37C

enhanceable

E(γTub37C¹)

(Bloomington Drosophila Stock Center, 1997.12.5)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

ncd

enhanceable

γTub37C

(Endow and Komma, 1998)

External Data

Subunit Structure (UniProtKB)

Interacts with Ote.

(UniProt, P42271 )

Linkouts

BioGRID - A database of protein and genetic interactions.

61183

DroID - A comprehensive database of gene and protein interactions.

FBgn0010097

MIST (genetic) - An integrated Molecular Interaction Database

35199

MIST (protein-protein) - An integrated Molecular Interaction Database

35199

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Motor proteins

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-54

Cytogenetic map

37C7-37C7

Sequence location

2L:19,183,863..19,185,726 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

37C7-37C7

Limits computationally determined from genome sequence between P{lacW}brat^k06028&P{lacW}l(2)k09613^k09613 and P{EP}dnt^EP2158

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

37C-37D

(determined by in situ hybridisation)

(Wilson et al., 1997)

37C-37C

(determined by in situ hybridisation)

(Tavosanis et al., 1997)

37C-37C

(Tavosanis et al., 1996)

37D-37D

(determined by in situ hybridisation)

(Bolwig et al., 1995)

Experimentally Determined Recombination Data

Location

2-54

(FlyBase, 2016, Schupbach and Wieschaus, 1989)

2-53.6

(Comeron et al., 2012)

(Tavosanis et al., 1996)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (17)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

4201

γTub37C³/CyO

56831

w¹¹¹⁸; P{ncd-γTub37C.GFP}F13F3

86005

w¹¹¹⁸; PBac{RB}γTub37C^e00793/CyO

57328

P{ncd-γTub37C.GFP}M5F1, w¹¹¹⁸/Dp(1;Y)Bar^S

91954

y¹ sc^* v¹ sev²¹; P{TKO.GS00165}attP40

107628

17566R-1

17566R-2

M2L-0161

y¹ w¹¹¹⁸; γTub37C^SK1 P{neoFRT}40A/CyO-Tb

M2L-0162

y¹ w¹¹¹⁸; γTub37C^SK2 P{neoFRT}40A/CyO-Tb

VDRC

v109921

P{KK110694}VIE-260B

v25271

w¹¹¹⁸; P{GD9416}v25271

More stocks available...

Genomic Clones (21)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (31)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD32755

Other clones

DPiM_LD32755

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0010097

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0010097

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Wiese, 2008)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

sub mutants do not interact with alleles of γTub37C, αTub67C, msps, tacc or wisp.

(Giunta et al., 2002)

γTub37C is essential for nuclear proliferation in the early embryo.

(Llamazares et al., 1999)

Phenotypic rescue of fs(2)TW1 mutants by a γTub37C construct demonstrates γTub37C and fs(2)TW1 are the same gene. This conclusion is substantiated further by the perfect agreement between the restriction maps.

(Tavosanis et al., 1997)

Expression of γTub37C is under strict developmental regulation and the organisation of the female meiotic spindle requires γTub37C. γTub37C gene product provides a function which is essential exclusively for female fertility.

(Tavosanis et al., 1997)

Immunoblot analysis and immunolocalisation show that γTub23C and γTub37C are differentially expressed during gametogenesis and development.

(Wilson et al., 1997)

Maternal effect mutant that affects early embryogenesis.

(Digilio et al., 1996)

Subcellular localisation of the protein during the cell cycle of cells with and without centrioles is determined. Data suggests centrioles are not essential to the formation of a functional mitotic spindle, but participate in the focal concentration of γTub23C and γTub37C protein during both interphase and mitosis.

(Debec et al., 1995)

Located between coordinates +50.1 and +60.1.

(Stathakis et al., 1995)

Mutations in γTub37C show a maternal effect with defects in fertilisation or very early embryonic development.

(Schupbach and Wieschaus, 1989)

Identification: Saturation mutagenesis of Df(2L)TW130.

Relationship to Other Genes

Source for database merge of

Additional comments

Eight independent occurrences recovered by Wright, four more by Schupbach.

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (49)

Reported As

Symbol Synonym

CG17566

(National Institute of Genetics Fly Stocks, 2022-, Neuert et al., 2017, Çiçek et al., 2016, Januschke et al., 2013, Müller et al., 2010, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Hughes et al., 2008, Goldstein and Gunawardena, 2000)

D.m.GAMMA-37C

(Goldstein and Gunawardena, 2000)

GTU88*

(Martinez-Campos et al., 2004)

PL10

(Digilio et al., 1996)

Rb1011

(Tavosanis and Gonzalez, 2003)

TW1

(Wright, 1996)

Tub37C

(Neuert et al., 2017)

Tub3B

(Kollmar, 2015.9.15, Kollmar and Odronitz, 2006)

fs(2)TW1

(Tadros et al., 2003, Tavosanis and Gonzalez, 2003, Wright, 1996, Stathakis et al., 1995, Schupbach and Wieschaus, 1989)

fs(2)TWI

(Gateff and Mechler, 1989)

fsTW1

(McCrady and Tolin, 1994)

g-Tubulin

(Hutterer et al., 2003)

gamma Tub37CD

(Wilson, 1998.9.8)

gamma tubulin

(Zhang and Megraw, 2006)

gammaTub37C

(National Institute of Genetics Fly Stocks, 2022-, Levis, 2018.8.30, Transgenic RNAi Project members, 2017-, Chen et al., 2016, Japanese National Institute of Genetics, 2012.5.21, Müller et al., 2010, Bakal et al., 2007, Giot et al., 2003)

γ tubulin

(Riparbelli et al., 2002)

γ-Tub

(Hayashi et al., 2014, Januschke et al., 2013, Lerit and Rusan, 2013, Habermann et al., 2012, Zhang and Megraw, 2007, Pelissier et al., 2003, de Carcer et al., 2001)

γ-Tub37C

(Hughes et al., 2018, Tovey et al., 2018, Rogers et al., 2008, Jang et al., 2005, Ephrussi and St. Johnston, 2004)

γ-tub

(Zheng et al., 2014)

γ-tub37C

(Colombié et al., 2013)

γ-tub37CD

(Raynaud-Messina et al., 2001)

γ-tubulin

(Gillard et al., 2021, Riparbelli et al., 2018, Romé and Ohkura, 2018, Tovey et al., 2018, Conduit et al., 2015, Bettencourt-Dias et al., 2004)

γ-tubulin37C

(Tovey et al., 2021)

γ^mat

(Wilson et al., 1992)

γTUB

(Herzig et al., 2002)

γTUB37C

(Janic et al., 2010, Tavosanis et al., 1997)

γTub

(Ricolo et al., 2021, Carmena, 2020, Sato et al., 2011, Orian et al., 2007, Barros et al., 2005, Pai et al., 2004)

γTub2

γTub37CD

(Wiese, 2008, Raynaud-Messina et al., 2004, Wilson, 1999.12.22, Wilson and Borisy, 1998, Wilson and Borisy, 1998)

γTub37c

(Czech et al., 2013)

γtub

(Harris and Peifer, 2004)

γtub37C

(Bu et al., 2022)

γtubulin

(Shoda et al., 2021)

Name Synonyms

37CD γ-tubulin

(Raynaud-Messina et al., 2004)

Gamma Tubulin at 37C

(Ducat et al., 2008)

female sterile(2)Ted Wright 1

g-tubulin37C

(Mukherjee et al., 2020)

gama-tubulin

(Moutinho-Santos et al., 2001)

gamma tubulin

(Donaldson et al., 2001, Grieder et al., 2000, Megraw et al., 2000)

gamma-tubulin

(Martins et al., 2007, Rusan and Peifer, 2007, Kaltschmidt and Brand, 2002, Jeng and Stearns, 1999, Vied and Horabin, 1999, Endow and Komma, 1998, Oakley, 1991.10.4)

gammaTub37C

(Williams et al., 2020)

maternal γ-tubulin

(Schnorrer et al., 2002)

tubulin

(Yuen et al., 2008, Ashley et al., 2005, Goshima et al., 2005)

γ Tubulin2

γ tubulin

(Harris and Peifer, 2005, Fischer et al., 2004, Stumpff et al., 2004, Goshima and Vale, 2003, Donaldson et al., 2001, Lee et al., 2001, Schaefer et al., 2001, Foe et al., 2000, Gunawardane et al., 2000, Avides and Glover, 1999, Zimmerman et al., 1999, Carmena et al., 1998, Helps et al., 1998, Moritz et al., 1998, Riparbelli et al., 1997)

γ-Tubulin

(Gopalakrishnan et al., 2011, Megraw et al., 2008, Rickmyre et al., 2007, Colombie et al., 2006, Riparbelli et al., 2004, Berdnik and Knoblich, 2002, Cha et al., 2002, Berdnik et al., 2001)

γ-Tubulin 37C

(Bernard et al., 2018)

γ-Tubulin at 37C

γ-tubulin

(Jana et al., 2018, Zheng et al., 2016, Conduit et al., 2014, Delgehyr et al., 2012, Gopalakrishnan et al., 2012, Ori-McKenney et al., 2012, Reschen et al., 2012, Sanghavi et al., 2012, Conduit and Raff, 2010, Bucciarelli et al., 2009, Blachon et al., 2008, Colombié et al., 2008, Goshima et al., 2008, Mottier et al., 2008, Tan et al., 2008, Trammell et al., 2008, Wei et al., 2008, Yang and Fan, 2008, Goshima et al., 2007, Peel et al., 2007, Rodrigues-Martins et al., 2007, Yamashita et al., 2007, Yamashita et al., 2007, Zhang et al., 2007, Verollet et al., 2006, Barros et al., 2005, Goshima et al., 2005, Januschke et al., 2005, Morales-Mulia and Scholey, 2005, Wilson, 2005, Cottam et al., 2004, Harris and Peifer, 2004, Kwon and Scholey, 2004, Roper and Brown, 2004, Salisbury, 2004, Wilson et al., 2004, Albertson and Doe, 2003, Blagden and Glover, 2003, Fuse et al., 2003, Gunawardane et al., 2003, Mathe et al., 2003, Silvanovich et al., 2003, Gergely, 2002, Mollinari et al., 2002, Bolivar et al., 2001, Ceron et al., 2001, Greaves, 2001, Huynh et al., 2001, Lee et al., 2001, MacDougall et al., 2001, Megraw et al., 2001, Ohkura et al., 2001, Wakefield et al., 2001, Afshar et al., 2000, Barbosa et al., 2000, Bonaccorsi et al., 2000, Brent et al., 2000, Grieder et al., 2000, Gunawardane et al., 2000, Lange et al., 2000, Lemos et al., 2000, Moritz et al., 2000, Riparbelli et al., 2000, Sibon et al., 2000, Su and Vidwans, 2000, Bamburg et al., 1999, Callaini et al., 1999, Gho et al., 1999, Gunawardane et al., 1999, Megraw et al., 1999, Moutinho-Santos et al., 1999, Oegema et al., 1999, Robinson et al., 1999, Swan et al., 1999, Vaizel-Ohayon and Schejter, 1999, Vidwans et al., 1999, Herrmann et al., 1998, Marshall, 1998, Riparbelli and Callaini, 1998, Callaini et al., 1997, Davis, 1997, Moritz et al., 1995, Lajoie-Mazenc et al., 1994, Raff et al., 1993, Moritz and Alberts, 1992)

γ-tubulin (37C)

(Mahoney et al., 2006)

γ-tubulin-2

(Debec et al., 1995)

γ-tubulin37C

(Colombié et al., 2013, Ori-McKenney et al., 2012, Parton et al., 2011)

γTubulin

(Wang and Riechmann, 2008)

γtubulin

(Leatherman and Dinardo, 2010, Terada et al., 2003, Wilson, 2001, Kaltschmidt et al., 2000, Wilson, 1999, Wilson, 1999, Craig and Brink, 1996)

Secondary FlyBase IDs

FBgn0001003

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 59 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35199

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P42271

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P42271

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

M9PDN9

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P42271

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0010097

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35199/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0010097

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0010097

FlyMine - An integrated database for Drosophila genomics

FBgn0010097

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG17566

MARRVEL_MODEL - MARRVEL (model organism gene)

35199

Linkouts

BioGRID - A database of protein and genetic interactions.

61183

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0010097

DroID - A comprehensive database of gene and protein interactions.

FBgn0010097

DRSC - Results frm RNAi screens

FBgn0010097

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

gammaTub37C_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0010097

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0010097

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG17566

Flygut - An atlas of the Drosophila adult midgut

FBgn0010097

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Motor proteins

MIST (genetic) - An integrated Molecular Interaction Database

35199

MIST (protein-protein) - An integrated Molecular Interaction Database

35199

References (316)

The following external sites may use different assemblies or annotations than FlyBase.

-0.30

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