FlyBase Gene Report: Dmel\pont

General Information

Symbol

Dmel\pont

Species

D. melanogaster

Name

pontin

Annotation Symbol

CG4003

Feature Type

protein_coding_gene

FlyBase ID

FBgn0040078

Gene Model Status

Current

Stock Availability

9 publicly available

Enzyme Name (EC)

DNA helicase (3.6.4.12)

Gene Summary

pontin (pont) encodes a member of the AAA+ (ATPases Associated with various cellular Activities) family. It is found in different complexes involved in transcriptional regulation, chromatin remodelling, DNA repair or ribonucleoprotein assembly, and participates in the control of cell proliferation and survival. [Date last reviewed: 2019-03-21] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Gene Group
UniProtKB
Interactive Fly

Also Known As

dPontin, Tip49/Pontin, Tip49, RUVBL1

Key Links

Genomic Location

Cytogenetic map

86A3-86A3

Sequence location

3R:10,262,123..10,263,899 [+]

Recombination map

3-49

RefSeq locus

NT_033777 REGION:10262123..10263899

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (22 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with UniProtKB:Q9V3K3

(Bellosta et al., 2005)

inferred from physical interaction with UniProtKB:Q9W4S7

(Bellosta et al., 2005)

enables transcription coactivator activity

inferred from mutant phenotype

(Bellosta et al., 2005, Bauer et al., 2000)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR010339

(InterPro Project Members, 2004-)

enables ATP-dependent activity, acting on DNA

inferred from electronic annotation with InterPro:IPR027238

(InterPro Project Members, 2004-)

enables DNA helicase activity

inferred from biological aspect of ancestor with PANTHER:PTN000120244

(GO Reference Genome Project, 2011-)

Biological Process (11 terms)

Terms Based on Experimental Evidence (8 terms)

CV Term

Evidence

References

involved_in DNA repair-dependent chromatin remodeling

inferred from direct assay

(Kusch et al., 2004)

involved_in mitotic spindle assembly

inferred from mutant phenotype

(Ducat et al., 2008)

involved_in negative regulation of JNK cascade

inferred from mutant phenotype

(Wang et al., 2018)

acts_upstream_of negative regulation of programmed cell death

inferred from genetic interaction with FLYBASE:egr; FB:FBgn0033483

(Wang et al., 2018)

involved_in negative regulation of tumor necrosis factor-mediated signaling pathway

inferred from genetic interaction with FLYBASE:egr; FB:FBgn0033483

(Wang et al., 2018)

involved_in positive regulation of canonical Wnt signaling pathway

inferred from mutant phenotype

(Bauer et al., 2000)

involved_in positive regulation of gene expression

inferred from mutant phenotype

(Diop et al., 2008)

involved_in regulation of cell population proliferation

inferred from mutant phenotype

(Bellosta et al., 2005)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in box C/D snoRNP assembly

inferred from biological aspect of ancestor with PANTHER:PTN000120244

(GO Reference Genome Project, 2011-)

involved_in chromatin remodeling

inferred from biological aspect of ancestor with PANTHER:PTN000120244

(GO Reference Genome Project, 2011-)

inferred by curator from GO:0031011

(Klymenko et al., 2006)

involved_in regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN000120244

(GO Reference Genome Project, 2011-)

Cellular Component (6 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

part_of brahma complex

inferred from direct assay

(Diop et al., 2008)

part_of Ino80 complex

inferred from direct assay

(Klymenko et al., 2006)

part_of NuA4 histone acetyltransferase complex

inferred from direct assay

(Kusch et al., 2004)

located_in nucleus

inferred from direct assay

(Bauer et al., 2000)

inferred from direct assay

(Kusch et al., 2004)

part_of R2TP complex

inferred from direct assay

(Lennon et al., 2022)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

part_of Ino80 complex

inferred from biological aspect of ancestor with PANTHER:PTN000120245

(GO Reference Genome Project, 2011-)

part_of NuA4 histone acetyltransferase complex

inferred from biological aspect of ancestor with PANTHER:PTN000120244

(GO Reference Genome Project, 2011-)

part_of R2TP complex

inferred from biological aspect of ancestor with PANTHER:PTN000120244

(GO Reference Genome Project, 2011-)

part_of Swr1 complex

inferred from biological aspect of ancestor with PANTHER:PTN000120245

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

INO80 COMPLEX

TIP60 COMPLEX

RUVB-LIKE DNA HELICASES

SWR1 COMPLEX

Pathway (FlyBase)

NEGATIVE REGULATORS OF TNFalpha-EIGER SIGNALING PATHWAY

Protein Family (UniProt)

Belongs to the RuvB family. (Q9VH07)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

DNA helicase activity

ATP + H2O = ADP + phosphate + H(+) (3.6.4.12)

Summaries

Gene Snapshot

Gene Group (FlyBase)

INO80 COMPLEX -: The INO80 complex is an ATP-dependent chromatin remodeller that can mobilize nucleosomes. (Adapted from PMID:17316710).
TIP60 COMPLEX -: The Tip60 chromatin remodeling complex acetylates nucleosomal phospho-histone H2Av and exchanges it with unmodified H2Av. (Adapted from FBrf0185742).
RUVB-LIKE DNA HELICASES -: RuvB-like DNA helicases are homologs of E. coli RuvB, comprising RVB1 and RVB2 (also known as Pontin/Reptin) in eukaryotes. They are two are two highly conserved members of the AAA+ family of ATPases. RuvB-like DNA helicases are part of various chromatin remodeling complexes where they regulate the accessibility of DNA to the proteins involved in transcription and DNA damage repair by regulating the position or modification of nucleosomes. (Adapted from PMID:19524533.)
SWR1 COMPLEX -: In D.mel, the SWR1 complex is an ATP-dependent histone exchanger that incorporates the histone H2A variant, His2Av. (Adapted from FBrf0245697.)

Pathway (FlyBase)

NEGATIVE REGULATORS OF TNFalpha-EIGER SIGNALING PATHWAY -: Negative regulators of Tumor Necrosis Factor α (TNFα) signaling pathway down-regulate the activation of the pathway triggered by Eiger (egr).

Protein Function (UniProtKB)

Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity (By similarity). Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of histone H4 'Lys-12' (PubMed:32432549). Component of a SWR1-like complex that incorporates H2A.V genome-wide and specifically at active promoters, in an ATP-dependent manner (PubMed:32432549). Component of the Tip60 chromatin-remodeling complex that upon induction of DNA double-strand breaks, mediates the ATP-dependent exchange of unmodified histone H2AV for its phosphorylated and acetylated form H2AVK5acS138ph, leading to transcriptional regulation of selected genes by chromatin remodeling (PubMed:15528408). Component of the chromatin-remodeling INO80 complex (By similarity). Binds beta-catenin, to positively regulate Wnt-responsive targets (PubMed:11080158). Also an essential cofactor for the normal function of Myc; required for cellular proliferation and growth (PubMed:16087886).

(UniProt, Q9VH07)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\pont for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VH07)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.43

Gene model reviewed during 5.48

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

pont-RA

FBtr0082226

NM_144351

1659

456

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

pont-PA

FBpp0081704

50.2

456

7.38

Q9VH07

NP_652608

AAF54514

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Forms homohexameric rings. May form a dodecamer with rept made of two stacked hexameric rings (By similarity). Component of the NuA4 histone acetyltransferase complex at least composed of dom/Domino, Brd8, MRG15, YL-1, MrgBP, pont/Pontin, rept/Reptin, DMAP1, Tip60, Ing3, Nipped-A, E(Pc), Gas41 and Bap55 (PubMed:32432549). Component of a SWR1-like complex at least composed of dom/Domino, Brd8, MrgBP, YL-1, Gas41, Bap55, Arp6, DMAP1, MRG15, pont/Pontin, rept/Reptin and Hcf (PubMed:32432549). Component of the Tip60 chromatin-remodeling complex which contains dom/Domino, Tip60, Tra1, Brd8, E(Pc), DMAP1, pont, rept, Ing3, Act87E, Bap55, MRG15, MrgBP, Gas41 and YL-1 (PubMed:15528408). Component of the chromatin remodeling Ino80 complex (PubMed:16618800). Interacts with Myc and rept (PubMed:16087886).

(UniProt, Q9VH07)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VH07

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\pont using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

pont_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

3.98

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism | ubiquitous

Comment: maternally contributed

(Bauer et al., 2000)

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 5

mesoderm anlage

(Bauer et al., 2000)

endoderm anlage

(Bauer et al., 2000)

embryonic stage 6 -- 8

cephalic furrow

(Bauer et al., 2000)

mesoderm

(Bauer et al., 2000)

posterior endoderm

(Bauer et al., 2000)

anterior endoderm

(Bauer et al., 2000)

embryonic stage 7 -- 8

anterior endoderm anlage

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

hindgut anlage

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

inclusive hindgut primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 9 -- 15

endodermal derivative

(Bauer et al., 2000)

embryonic stage 11 -- 14

trunk mesoderm

(Bauer et al., 2000)

embryonic stage 13 -- 16

dorsal sensory complex proper primordium

Comment: reported as dorsal/lateral sensory complexes

(Fisher et al., 2012)

embryonic/larval fat body

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

larval muscle system

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

sensory nervous system primordium

(Fisher et al., 2012)

ventral sensory complex primordium

(Fisher et al., 2012)

embryonic stage 15 -- 17

chordotonal organ precursor cell

(Bauer et al., 2000)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 14 -- 17

endodermal derivative

• nucleus

(Bauer et al., 2000)

early third instar larval stage

embryonic/larval fat body

(Banreti et al., 2014)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism

(Bauer et al., 2000)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of brahma complex

inferred from direct assay

(Diop et al., 2008)

part_of Ino80 complex

inferred from direct assay

(Klymenko et al., 2006)

part_of NuA4 histone acetyltransferase complex

inferred from direct assay

(Kusch et al., 2004)

located_in nucleus

inferred from direct assay

(Bauer et al., 2000)

inferred from direct assay

(Kusch et al., 2004)

part_of R2TP complex

inferred from direct assay

(Lennon et al., 2022)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\pont in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\pont

View exonic expression by tissue for Dmel\pont

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0040078

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

53439/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0040078

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0040078

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0040078

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4003

Flygut - An atlas of the Drosophila adult midgut

FBgn0040078

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\pont

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 12 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of pont

Transgenic constructs containing regulatory region of pont

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene not disrupted in

Df(3R)Delta-BX12

(Szeged Stock Center, 1998-)

Gene disrupted in

Df(3R)Exel6156

(Ryder, 2004.4.26)

Inferred from location ( 4 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^c564

pont^KK101103

lethal, with Scer\GAL4^sca.PU

pont^KK101103

lethal | recessive

pont^S022905a

lethal - all die before end of larval stage, with Scer\GAL4^Tub.PU

pont^KK101103

lethal - all die before end of larval stage | recessive

pont^5.1

Sterility

Allele

male fertile, with Scer\GAL4^bam.PU

pont^HMJ21078

male sterile, with Scer\GAL4^VP16.bam

pont^KK101103

Other Phenotypes

Allele

abnormal circadian rhythm | adult stage, with Scer\GAL4^ey-OK107

pont^KK101103

abnormal cytokinesis | spermatogenesis, with Scer\GAL4^VP16.bam

pont^KK101103

abnormal developmental rate | somatic clone

pont^5.1

decreased cell number | adult stage, with Scer\GAL4^Tk.PS

pont^HMJ21078

decreased cell number | male, with Scer\GAL4^VP16.bam

pont^KK101103

decreased size | adult stage, with Scer\GAL4^ey.PU

pont^KK101103

increased sleep | adult stage | female, with Scer\GAL4^ey-OK107

pont^KK101103

some die during larval stage | recessive

pont^5.1

suppressor of variegation | dominant, with In(1)w^m4

pont^5.1

visible, with Scer\GAL4^arm.PS

pont^D302N.UAS

visible | adult stage, with Scer\GAL4^ey.PU

pont^KK101103

Phenotype manifest in

Allele

adult anterior midgut, with Scer\GAL4^Tk.PS

pont^HMJ21078

adult enteroendocrine cell | decreased number, with Scer\GAL4^Tk.PS

pont^HMJ21078

chromatin | spermatogenesis, with Scer\GAL4^VP16.bam

pont^KK101103

embryonic/larval salivary gland | third instar larval stage, with Scer\GAL4^Tub.PU

pont^KK101103

eye, with Scer\GAL4^ey.PU

pont^KK101103

lipid droplet | adult stage, with Scer\GAL4^Tk.PS

pont^HMJ21078

meiotic spindle | spermatogenesis, with Scer\GAL4^VP16.bam

pont^KK101103

meiotic spindle pole | increased number | spermatogenesis, with Scer\GAL4^VP16.bam

pont^KK101103

polytene chromosome | third instar larval stage, with Scer\GAL4^Tub.PU

pont^KK101103

spermatid, with Scer\GAL4^VP16.bam

pont^KK101103

spermatid axoneme, with Scer\GAL4^VP16.bam

pont^KK101103

spermatozoon, with Scer\GAL4^VP16.bam

pont^KK101103

wing, with Scer\GAL4^arm.PS

pont^D302N.UAS

wing | somatic clone

pont^5.1

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (5)

Hsap\RUVBL1

14 of 14

Yes

3 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (5)

Rnor\Ruvbl1

14 of 14

Yes

Rnor\RGD1564855

11 of 14

Yes

Rnor\Ruvbl2

3 of 14

Rnor\Spata5

1 of 14

Rnor\Yme1l1

1 of 14

Mus musculus (laboratory mouse) (4)

Mmus\Ruvbl1

13 of 14

Yes

Mmus\Ruvbl2

3 of 14

Mmus\Spata5

1 of 14

Mmus\Yme1l1

1 of 14

Xenopus tropicalis (Western clawed frog) (2)

Xtro\ruvbl1

11 of 13

Yes

Xtro\ruvbl2

1 of 13

Danio rerio (Zebrafish) (4)

Drer\ruvbl1

14 of 14

Yes

Drer\ruvbl2

3 of 14

Drer\yme1l1a

1 of 14

Drer\yme1l1b

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\ruvb-1

14 of 14

Yes

Cele\ruvb-2

3 of 14

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP009746

3 of 12

Yes

Arabidopsis thaliana (thale-cress) (4)

Atha\RIN1

13 of 13

Yes

Atha\AT3G49830

3 of 13

Atha\AT5G67630

3 of 13

Atha\AT1G05910

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\RVB1

13 of 13

Yes

Scer\RVB2

2 of 13

Scer\YTA7

1 of 13

Schizosaccharomyces pombe (Fission yeast) (4)

Spom\rvb1

12 of 12

Yes

Spom\rvb2

2 of 12

Spom\cdc48

1 of 12

Spom\SPAC31G5.19

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:pont. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (2)

rept

5 of 13

smid

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

pont^KK101103

ameliorates acute myeloid leukemia

modeled by Hsap\RUNX1::Hsap\RUNX1T1^UAS.cMa

(Breig et al., 2014)

pont^{UAS.ORF.Tag:HA}

ameliorates C9orf72 frontotemporal dementia and/or amyotrophic lateral sclerosis

modeled by Zzzz\poly-GR^{1136.UAS.EGFP}

(Webster et al., 2025)

modeled by Zzzz\poly-PR^{1100.UAS.EGFP}

(Webster et al., 2025)

modeled by Zzzz\poly-PA^{1024.UAS.EGFP}

(Webster et al., 2025)

pont^NIG.4003R

ameliorates acute myeloid leukemia

modeled by Hsap\RUNX1::Hsap\RUNX1T1^UAS.cMa

(Breig et al., 2014)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RUVBL1; RuvB like AAA ATPase 1

14 of 14

603449

RUVBL2; RuvB like AAA ATPase 2

3 of 14

604788

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

pont - Act87E

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kusch et al., 2004)

pont - Bap55

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kusch et al., 2004)

pont - Brd8

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kusch et al., 2004)

pont - CG13901

experimental knowledge based

(Guruharsha et al., 2011)

pont - CG8745

experimental knowledge based

(Guruharsha et al., 2011)

pont - DMAP1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kusch et al., 2004)

pont - Dnaaf4

anti tag coimmunoprecipitation, anti tag western blot, pull down, Identification by mass spectrometry

(Lennon et al., 2022)

pont - Dnaaf6

anti tag coimmunoprecipitation, anti tag western blot

(Lennon et al., 2022)

pont - E(Pc)

anti tag coimmunoprecipitation, peptide massfingerprinting, western blot

(Kusch et al., 2004)

pont - Eaf6

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kusch et al., 2004)

pont - Gas41

anti tag coimmunoprecipitation, peptide massfingerprinting, western blot

(Kusch et al., 2004)

pont - His2Av

anti tag coimmunoprecipitation, western blot, peptide massfingerprinting

(Kusch et al., 2004)

pont - His2B

anti tag coimmunoprecipitation, western blot, peptide massfingerprinting

(Kusch et al., 2004)

pont - Ing3

anti tag coimmunoprecipitation, peptide massfingerprinting, western blot

(Kusch et al., 2004)

pont - MRG15

anti tag coimmunoprecipitation, western blot, peptide massfingerprinting

(Kusch et al., 2004)

pont - Mapmodulin

experimental knowledge based

(Guruharsha et al., 2011)

pont - MrgBP

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kusch et al., 2004)

pont - Myc

anti bait coimmunoprecipitation, western blot, pull down, anti tag coimmunoprecipitation

(Bellosta et al., 2005)

pont - Nipped-A

anti tag coimmunoprecipitation, peptide massfingerprinting, western blot

(Kusch et al., 2004)

pont - Pih1D1

anti tag coimmunoprecipitation, western blot

(Benbahouche et al., 2014)

pont - S6kII

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

pont - Tip60

anti bait coimmunoprecipitation, anti tag western blot, anti tag coimmunoprecipitation, western blot, peptide massfingerprinting

(Kusch et al., 2004)

pont - Tti1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Glatter et al., 2011)

pont - Wdr92

anti tag coimmunoprecipitation, anti tag western blot

(Zur Lage et al., 2018)

pont - YL-1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kusch et al., 2004)

pont - dom

anti tag coimmunoprecipitation, peptide massfingerprinting, western blot

(Kusch et al., 2004)

pont - ecd

anti tag coimmunoprecipitation, Identification by mass spectrometry, anti tag western blot

(Erkelenz et al., 2021)

pont - mTor

anti tag coimmunoprecipitation, peptide massfingerprinting

(Glatter et al., 2011)

pont - per

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Liao et al., 2025)

pont - pho

anti tag coimmunoprecipitation, western blot, peptide massfingerprinting

(Klymenko et al., 2006)

pont - rept

anti tag coimmunoprecipitation, anti tag western blot, western blot, experimental knowledge based, peptide massfingerprinting

(Liao et al., 2025, Guruharsha et al., 2011, Bellosta et al., 2005, Kusch et al., 2004)

pont - spag

anti tag coimmunoprecipitation, peptide massfingerprinting

(Benbahouche et al., 2014)

pont - wcy

anti tag coimmunoprecipitation, peptide massfingerprinting

(David-Morrison et al., 2016)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

arm

suppressible

pont

(Bauer et al., 2000)

brm

enhanceable

pont

(Diop et al., 2008)

Myc

enhanceable

pont

(Bellosta et al., 2005)

Myc

suppressible

pont

(Bellosta et al., 2005)

shg

enhanceable

pont

(Bauer et al., 2000)

External Data

Subunit Structure (UniProtKB)

(UniProt, Q9VH07 )

Linkouts

BioGRID - A database of protein and genetic interactions.

72727

DroID - A comprehensive database of gene and protein interactions.

FBgn0040078

MIST (genetic) - An integrated Molecular Interaction Database

53439

MIST (protein-protein) - An integrated Molecular Interaction Database

53439

Pathways

Signaling Pathways (FlyBase)

NEGATIVE REGULATORS OF TNFalpha-EIGER SIGNALING PATHWAY

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-49

Cytogenetic map

86A3-86A3

Sequence location

3R:10,262,123..10,263,899 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

86A3-86A3

Limits computationally determined from genome sequence between P{EP}Fmr1^EP3517&P{PZ}tws⁰²⁴¹⁴ and P{PZ}jumu⁰⁶⁴³⁹&P{lacW}l(3)j8B6^j8B6

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

86A-86A

(Chauvet, 2000.2.9)

91F-91F

(determined by in situ hybridisation)

(Szeged Stock Center, 1998-)

86A-86A

(determined by in situ hybridisation)

(Deak et al., 1997)

Experimentally Determined Recombination Data

Location

3-49

(FlyBase, 2016)

3-46.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (9)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

50972

y¹ v¹; P{TRiP.HMJ21078}attP40

64756

w^*; P{neoFRT}82B pont^5.1/TM3, Sb¹

82808

y¹ sc^* v¹ sev²¹; P{TKO.GS04167}attP40

81719

y¹ sc^* v¹ sev²¹; P{SAM.dCas9.GS03995}attP40

FlyORF

F000819

M{UAS-pont.ORF.3xHA}ZH-86Fb

NIG-Fly

4003R-1

P{NIG.4003R}1

HMJ21078

y¹ v¹; P{TRiP.HMJ21078}attP40/CyO

VDRC

v330324

P{VSH330324}attP40

v105408

P{KK101103}VIE-260B

Genomic Clones (7)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (157)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD08555

Other clones

DPiM_LD08555

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0040078

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0040078

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Fingerhut and Yamashita, 2020, Diop et al., 2008, Ducat et al., 2008, Bauer et al., 2000)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Source for merge of: pontin CG4003

(Bayraktaroglu, 2000.8.7)

Source for merge of: l(3)S022905 pontin

(Bauer et al., 2000)

Source for merge of: pont anon-WO0118547.698

(FlyBase, 1996-)

Additional comments

Source for merge of pont anon-WO0118547.698 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: pont pontin

(Pradel, 2001.1.25)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (19)

Reported As

Symbol Synonym

0229/05

(Deak et al., 1997)

CG4003

(Zur Lage et al., 2018, Meadows, 2017.1.11, Çiçek et al., 2016, Kumar et al., 2016, Rosa-Ferreira et al., 2015, Benbahouche et al., 2014, Schertel et al., 2013, Glatter et al., 2011, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Pan and Zhang, 2009, Ducat et al., 2008, Hughes et al., 2008, Kusch et al., 2004)

PON

(Bouazoune and Brehm, 2006)

PONT

(Fukuyama et al., 2013, Kondo and Perrimon, 2011)

Pont

(Rust et al., 2018, Sharma et al., 2017, David-Morrison et al., 2016, Banreti et al., 2014, Klymenko et al., 2006)

Pontin

(Liao et al., 2025, Clapier and Cairns, 2009, Stadeli, 2006)

RUVBL1

(Skayneh et al., 2019, Breig et al., 2014)

Tip49

(Stadeli, 2006, Hulf and Gallant, 2003)

Tip49/Pontin

(Bellosta et al., 2005, Gallant et al., 2003)

anon-WO0118547.698

dPon

(Kusch et al., 2004)

dPontin

(Bailetti et al., 2019, Kusch et al., 2004, Kusch et al., 2004)

dRUVBL1

(Yasunaga et al., 2014)

dpon

(Bauer et al., 2000)

l(3)S022905

pon

(Bauer et al., 2000)

pont

(François et al., 2023, Zheng et al., 2023, Sureka and Mishra, 2021, Fingerhut et al., 2019, Götze et al., 2019, McCarthy et al., 2018, Wang et al., 2018, Transgenic RNAi Project members, 2017-, Kwong et al., 2015, Kwon et al., 2013, Schertel et al., 2013, Wang et al., 2013, Antao et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Friedman et al., 2011, Lindquist et al., 2011, Toku et al., 2011, Diop et al., 2008)

pontin

(Vinayagam et al., 2016)

Name Synonyms

Pontin

(Lennon et al., 2022, Prozzillo et al., 2021, Flegel et al., 2016, Banreti et al., 2014, Benbahouche et al., 2014, Garcia and Stathopoulos, 2011, Dekanty et al., 2010, Huen et al., 2010, Ducat et al., 2008, Fisher et al., 2008, Ullah et al., 2007, Klymenko et al., 2006)

dPontin

(Jin, 2005)

dpontin

(Bauer et al., 2000)

pontin

(Zur Lage et al., 2019, Chen et al., 2018, Zur Lage et al., 2018, Perez-Perri et al., 2016, Breig et al., 2014, Yasunaga et al., 2014, Fukuyama et al., 2013, Vorobyeva et al., 2009, Diop et al., 2008)

Secondary FlyBase IDs

FBgn0028192
FBgn0037803
FBgn0061742

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 46 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

53439

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VH07

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VH07

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VH07

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0040078

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

53439/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0040078

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0040078

FlyMine - An integrated database for Drosophila genomics

FBgn0040078

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG4003

MARRVEL_MODEL - MARRVEL (model organism gene)

53439

Linkouts

BioGRID - A database of protein and genetic interactions.

72727

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0040078

DroID - A comprehensive database of gene and protein interactions.

FBgn0040078

DRSC - Results frm RNAi screens

FBgn0040078

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

pont_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0040078

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0040078

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4003

Flygut - An atlas of the Drosophila adult midgut

FBgn0040078

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0040078

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC012184

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (genetic) - An integrated Molecular Interaction Database

53439

MIST (protein-protein) - An integrated Molecular Interaction Database

53439

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (118)

The following external sites may use different assemblies or annotations than FlyBase.

3.98

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