FlyBase Gene Report: Dmel\didum

General Information

Symbol

Dmel\didum

Species

D. melanogaster

Name

dilute class unconventional myosin

Annotation Symbol

CG2146

Feature Type

protein_coding_gene

FlyBase ID

FBgn0261397

Gene Model Status

Current

Stock Availability

17 publicly available

Enzyme Name (EC)

myosin ATPase (5.6.1.8)

Gene Summary

dilute class unconventional myosin (didum) encodes a class V unconventional myosin that binds actin and hydrolyzes ATP. It functions in mRNA localization in the oocyte, spermatid individualization during spermatogenesis, transport of pigment granules in photoreceptors and mitochondrial transport in neuronal cells. [Date last reviewed: 2019-02-28] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

MyoV, myosin V, shorty, MyoV43CD, myosin-V

Key Links

Genomic Location

Cytogenetic map

43C7-43D1

Sequence location

2R:7,500,147..7,508,625 [+]

Recombination map

2-56

RefSeq locus

NT_033778 REGION:7500147..7508625

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (25 terms)

Molecular Function (8 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

enables actin binding

inferred from direct assay

(Toth et al., 2005)

enables ATP binding

inferred from direct assay

(Toth et al., 2005)

enables calcium-dependent ATPase activity

inferred from direct assay

(Ji et al., 2015)

enables calmodulin binding

inferred from physical interaction with FLYBASE:Cam; FB:FBgn0000253

(Martin and Bayley, 2004)

enables microfilament motor activity

inferred from direct assay

(Ji et al., 2015, Toth et al., 2005)

enables myosin light chain binding

inferred from physical interaction with FLYBASE:Cam; FB:FBgn0000253

(Toth et al., 2005)

inferred from physical interaction with FLYBASE:Mlc-c; FB:FBgn0004687

(Franke et al., 2006, Toth et al., 2005)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

enables actin binding

inferred from sequence or structural similarity with MGI:MGI:105976

(Bonafe and Sellers, 1998, MacIver et al., 1998)

enables actin filament binding

inferred from biological aspect of ancestor with PANTHER:PTN000320364

(GO Reference Genome Project, 2011-)

enables ATP binding

inferred from sequence or structural similarity with MGI:MGI:105976

(Bonafe and Sellers, 1998, MacIver et al., 1998)

inferred from electronic annotation with InterPro:IPR001609

(InterPro Project Members, 2004-)

enables cytoskeletal motor activity

inferred from electronic annotation with InterPro:IPR001609

(InterPro Project Members, 2004-)

enables microfilament motor activity

inferred from biological aspect of ancestor with PANTHER:PTN000320364

(GO Reference Genome Project, 2011-)

Biological Process (10 terms)

Terms Based on Experimental Evidence (8 terms)

CV Term

Evidence

References

involved_in actin filament-based movement

inferred from direct assay

(Toth et al., 2005)

involved_in anterior/posterior axis specification, embryo

inferred from high throughput mutant phenotype

(Luschnig et al., 2004)

involved_in intracellular protein transport

inferred from mutant phenotype

(Pocha et al., 2011)

inferred from genetic interaction with FLYBASE:crb; FB:FBgn0259685

(Pocha et al., 2011)

involved_in mitochondrion transport along microtubule

inferred from direct assay

(Pathak et al., 2010)

involved_in positive regulation of pole plasm oskar mRNA localization

inferred from mutant phenotype

(Krauss et al., 2009)

involved_in rhabdomere development

inferred from mutant phenotype

(Li et al., 2007)

involved_in sperm individualization

inferred from mutant phenotype

(Mermall et al., 2005)

NOT involved_in oogenesis

inferred from mutant phenotype

(Mermall et al., 2005)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in actin filament organization

inferred from biological aspect of ancestor with PANTHER:PTN000320364

(GO Reference Genome Project, 2011-)

involved_in endocytosis

inferred from biological aspect of ancestor with PANTHER:PTN008546178

(GO Reference Genome Project, 2011-)

Cellular Component (7 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

part_of myosin V complex

inferred from physical interaction with FLYBASE:Mlc-c; FB:FBgn0004687

(Franke et al., 2006)

inferred from physical interaction with FLYBASE:Cam; FB:FBgn0000253

(Martin and Bayley, 2004)

located_in neuronal cell body

inferred from direct assay

(Pocha et al., 2011)

located_in rhabdomere

inferred from direct assay

(Pocha et al., 2011)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

is_active_in actin cytoskeleton

inferred from biological aspect of ancestor with PANTHER:PTN000320364

(GO Reference Genome Project, 2011-)

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000320364

(GO Reference Genome Project, 2011-)

is_active_in membrane

inferred from biological aspect of ancestor with PANTHER:PTN000320364

(GO Reference Genome Project, 2011-)

part_of myosin complex

inferred from sequence or structural similarity with MGI:MGI:105976

(Bonafe and Sellers, 1998, MacIver et al., 1998)

inferred from electronic annotation with InterPro:IPR001609

(InterPro Project Members, 2004-)

Gene Group (FlyBase)

MYOSIN MOTORS

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

microfilament motor activity

ATP + H2O + myosin bound to actin filament at position(n) = ADP + phosphate + myosin bound to actin filament at position(n+1) (5.6.1.8)

Summaries

Gene Snapshot

Gene Group (FlyBase)

MYOSIN MOTORS -: Myosin motors are motor proteins that use the hydrolysis of ATP to drive movement along actin filaments. They can be divided into two groups: conventional myosins (myosin II) are involved in generating the mechanical force for muscle contraction; unconventional myosins (all other classes) are involved in processes such as cell motility, cytokinesis and trafficking. (Adapted from FBrf0134714.)

Summary (Interactive Fly)

a motor protein that mediates sensory neuron morphogenesisin - forms a complex with Rab11 and Rab11 interacting protein that trafficks rhodopsin in the developing rhabdomere

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\didum for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A1Z6Z8)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.48

Low-frequency RNA-Seq exon junction(s) not annotated.

Alternative translation stop created by use of multiphasic reading frames within coding region.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

didum-RA

FBtr0089745

NM_057838

6478

1792

didum-RB

FBtr0089744

NM_165536

5992

1196

didum-RC

FBtr0089743

NM_165535

6114

1800

Additional Transcript Data and Comments

Reported size (kB)

6.0 (northern blot)

(Bonafe and Sellers, 1998)

6.5 (northern blot)

(MacIver et al., 1998)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

didum-PA

FBpp0088686

207.3

1792

8.52

FBpp0088685

137.9

1196

9.45

FBpp0088684

208.2

1800

8.60

A1Z6Z8

NP_724569

AAM68902

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

1792 (aa); 207 (kD predicted)

(Bonafe and Sellers, 1998)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\didum using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

didum_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.81

Transcript Expression

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(MacIver et al., 1998)

embryonic stage & adult stage

(Bonafe and Sellers, 1998)

embryonic stage 1 -- 6

organism | ubiquitous

(Bonafe and Sellers, 1998)

embryonic stage 16

embryonic/larval hindgut

(Bonafe and Sellers, 1998)

adult stage | male

testis

(MacIver et al., 1998)

adult stage | female

ovary

(MacIver et al., 1998)

adult stage & oogenesis

oocyte

(MacIver et al., 1998)

adult stage oogenesis stage S6 -- S8

oocyte | anterior

(MacIver et al., 1998)

adult stage oogenesis stage S10 -- S14

nurse cell

(MacIver et al., 1998)

Additional Descriptive Data

didum transcripts are detected in embryos and adults on northern blots. Maternally derived transcripts are uniformly distributed in embryos up to stage 6. Later in embryogenesis, high levels of didum transcript are observed in the ectodermal tissue of the hindgut.

(Bonafe and Sellers, 1998)

didum transcripts are detected in adult ovaries and testes and at low levels in embryos by northern blots. They are detected by in situ hybridization in ovaries. They are localized to the oocyte from germarial stage 2 and are confined to the anterior margin of the oocyte during the middle stages of oogenesis (6-8). In late vitellogenic stages (9-11), there is strong staining in nurse cells and a loss of anterior localization in the oocyte.

(MacIver et al., 1998)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of myosin V complex

inferred from physical interaction with FLYBASE:Mlc-c; FB:FBgn0004687

(Franke et al., 2006)

inferred from physical interaction with FLYBASE:Cam; FB:FBgn0000253

(Martin and Bayley, 2004)

located_in neuronal cell body

inferred from direct assay

(Pocha et al., 2011)

located_in rhabdomere

inferred from direct assay

(Pocha et al., 2011)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\didum in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\didum

View exonic expression by tissue for Dmel\didum

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35680/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0261397

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0015933

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0261397

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2146

Flygut - An atlas of the Drosophila adult midgut

FBgn0261397

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 11 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 19 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of didum

Transgenic constructs containing regulatory region of didum

Aberrations (Deficiencies and Duplications) ( 4 )

Inferred from experimentation ( 4 )

Gene disrupted in

Df(2R)del28

(Mermall et al., 2005)

Df(2R)Drl^rv21

(Mermall et al., 2005)

Df(2R)Drl^rv22

(Mermall et al., 2005)

Df(2R)Exel6052

(Ryder, 2004.4.26)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die before end of larval stage | recessive

lethal - all die during embryonic stage | germline clone | maternal effect | recessive

lethal - all die during embryonic stage | germline clone | non-rescuable maternal effect | recessive

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

didum^GD1848

viable, with Scer\GAL4^pnr-MD237

didum^GD1848

viable, with Scer\GAL4^tin.CΔ4

didum^GD1848

Sterility

Allele

female fertile

didum^Q1052st

female fertile | germline clone

didum^Q1052st

male sterile | recessive

didum^Q1052st

Other Phenotypes

Allele

abnormal axis specification | germline clone | oogenesis

didum²³⁴

abnormal cell polarity | germline clone | oogenesis

didum²³⁴

abnormal developmental rate

didum^unspecified

abnormal developmental rate | recessive

didum^Q1052st

some die during embryonic stage | maternal effect | recessive

some die during embryonic stage | non-rescuable maternal effect | recessive

some die during larval stage | recessive

didum^unspecified

some die during third instar larval stage | recessive

didum^Q1052st

Phenotype manifest in

Allele

embryo | germline clone | maternal effect | posterior

embryonic abdomen | germline clone | maternal effect

didum¹⁵⁴

embryonic abdominal segment | germline clone | maternal effect

didum^unspecified

embryonic head | germline clone | maternal effect

eye photoreceptor cell | somatic clone

didum^Q1052st

filzkorper | germline clone | maternal effect

male genitalia, with Scer\GAL4^Abd-B-LDN

didum^GD1848

male genitalia, with Scer\GAL4^tsh-md621

didum^GD1848

oocyte | germline clone

didum²³⁴

ovary | germline clone | maternal effect

primordial germ cell | germline clone | maternal effect

rhabdomere | ectopic

didum^KG04384

rhabdomere | ectopic | somatic clone

didum^Q1052st

rhabdomere | somatic clone

didum^Q1052st

spermatid cyst

didum^Q1052st

spermatozoon

didum^Q1052st

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (45)

Hsap\MYO5A

13 of 14

Yes

Hsap\MYO5C

12 of 14

Yes

Hsap\MYO5B

11 of 14

Yes

Hsap\MYO19

4 of 14

Yes

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\RASIP1

1 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (33)

Rnor\Myo5a

12 of 14

Yes

Rnor\Myo5b

12 of 14

Yes

Rnor\Myo5c

11 of 14

Yes

Rnor\Myo19

4 of 14

Yes

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Rasip1

1 of 14

Yes

Mus musculus (laboratory mouse) (36)

Mmus\Myo5a

13 of 14

Yes

Mmus\Myo5b

12 of 14

Yes

Mmus\Myo5c

12 of 14

Yes

Mmus\Myo19

4 of 14

Yes

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Rasip1

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (32)

Xtro\myo5a

11 of 13

Yes

Xtro\myo5b

6 of 13

Yes

Xtro\rpl11

5 of 13

Xtro\myo5c

4 of 13

Yes

Xtro\myo19

3 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\st6galnac4

1 of 13

Danio rerio (Zebrafish) (51)

Drer\myo5aa

12 of 14

Yes

Drer\myo5ab

12 of 14

Yes

Drer\myo5b

11 of 14

Yes

Drer\myo5c

11 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\rasip1

1 of 14

Yes

Drer\si:ch211-18i17.2

1 of 14

Drer\si:dkey-110c1.10

1 of 14

Yes

Drer\zgc:66156

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (19)

Cele\hum-2

13 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (14)

Agam\AgaP_AGAP006479

12 of 12

Yes

3 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (20)

Atha\XIC

11 of 13

Yes

Atha\XIE

11 of 13

Yes

Atha\MYA1

10 of 13

Yes

Atha\MYA2

10 of 13

Yes

Atha\XIA

10 of 13

Yes

Atha\XIB

10 of 13

Yes

Atha\XID

10 of 13

Yes

Atha\XIF

10 of 13

Yes

Atha\XIG

10 of 13

Yes

Atha\XIH

10 of 13

Yes

Atha\XI-I

10 of 13

Yes

Atha\XIJ

10 of 13

Yes

Atha\XIK

10 of 13

Yes

Atha\ATM1

7 of 13

Yes

Atha\ATM2

7 of 13

Yes

Atha\VIIIA

7 of 13

Yes

Atha\VIIIB

6 of 13

Yes

Atha\AT5G20450

2 of 13

Yes

Atha\HDK

2 of 13

Yes

Atha\AT1G42680

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (6)

Scer\MYO2

12 of 13

Yes

Scer\MYO4

10 of 13

Yes

Scer\MYO1

2 of 13

Scer\YPR089W

2 of 13

Yes

Scer\MYO3

1 of 13

Scer\MYO5

1 of 13

Schizosaccharomyces pombe (Fission yeast) (5)

Spom\myo52

10 of 12

Yes

Spom\myo51

9 of 12

Yes

Spom\myo2

2 of 12

Spom\SPAC25B8.08

2 of 12

Yes

Spom\myp2

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:didum. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (17)

Myo10A

6 of 13

5 of 13

dachs

5 of 13

Myo28B1

5 of 13

jar

4 of 13

Mhc

4 of 13

zip

4 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

Prm

2 of 13

CG31638

1 of 13

CG43796

1 of 13

cno

1 of 13

ninaC

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 2 )

Human Ortholog

Disease

Evidence

References

MYO5A; myosin VA

model of Griscelli syndrome type 1

IEA

(FlyBase, 2019-)

MYO5B; myosin VB

model of microvillus inclusion disease

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

MYO5A; myosin VA

13 of 14

160777

GRISCELLI SYNDROME, TYPE 1; GS1

Griscelli syndrome type 1

12 of 14

11 of 14

microvillus inclusion disease

MYO19; myosin XIX

4 of 14

617379

MYH10; myosin heavy chain 10

3 of 14

160776

MYO15A; myosin XVA

3 of 14

602666

DEAFNESS, AUTOSOMAL RECESSIVE 3; DFNB3

autosomal recessive nonsyndromic deafness 3

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

didum - Act57B

experimental knowledge based

(Guruharsha et al., 2011)

didum - Arp2

experimental knowledge based

(Guruharsha et al., 2011)

didum - Arpc2

experimental knowledge based

(Guruharsha et al., 2011)

didum - CG7611

pull down, anti tag western blot

(Finan et al., 2011)

didum - Cam

anti tag coimmunoprecipitation, western blot, peptide massfingerprinting

(Satoh et al., 2008, Toth et al., 2005)

didum - Kaz

pull down, anti tag western blot

(Finan et al., 2011)

didum - Khc

two hybrid

(Krauss et al., 2009)

didum - Mer

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

didum - Mlc-c

anti tag coimmunoprecipitation, Identification by mass spectrometry, anti bait coimmunoprecipitation, peptide massfingerprinting, western blot

(Satoh et al., 2008, Franke et al., 2006, Toth et al., 2005)

didum - Ntan1

experimental knowledge based

(Guruharsha et al., 2011)

didum - Nup93-1

pull down, anti tag western blot

(Finan et al., 2011)

didum - Patj

anti bait coimmunoprecipitation, peptide massfingerprinting, western blot

(Pocha et al., 2011)

didum - Rab11

pull down, peptide massfingerprinting, anti tag coimmunoprecipitation, autoradiography, two hybrid, anti tag western blot

(Ji et al., 2015, Ramel et al., 2013, Satoh et al., 2008, Li et al., 2007)

didum - Rab32

two hybrid, pull down, anti tag western blot, anti tag coimmunoprecipitation, autoradiography

(Ji et al., 2015, Satoh et al., 2008)

didum - SMC5

anti tag coimmunoprecipitation, anti tag western blot

(Caridi et al., 2018)

didum - SMC6

anti tag coimmunoprecipitation, anti tag western blot

(Caridi et al., 2018)

didum - Swip-1

experimental knowledge based

(Guruharsha et al., 2011)

didum - cpa

experimental knowledge based

(Guruharsha et al., 2011)

didum - cpb

experimental knowledge based

(Guruharsha et al., 2011)

didum - jar

anti bait coimmunoprecipitation, western blot, pull down, peptide massfingerprinting

(Finan et al., 2011)

didum - osk

anti tag coimmunoprecipitation, quantitative reverse transcription pcr, Identification by mass spectrometry

(Hurd et al., 2016, Krauss et al., 2009)

didum - sdt

anti bait coimmunoprecipitation, peptide massfingerprinting, western blot

(Pocha et al., 2011)

didum - stau

anti tag coimmunoprecipitation, western blot

(Lu et al., 2018)

RNA-protein

Physical Interaction

Assay

References

didum - osk

anti tag coimmunoprecipitation, quantitative reverse transcription pcr, Identification by mass spectrometry

(Hurd et al., 2016, Krauss et al., 2009)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

fzr

suppressible

didum

(Neuert et al., 2017)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

61556

DroID - A comprehensive database of gene and protein interactions.

FBgn0261397

MIST (protein-protein) - An integrated Molecular Interaction Database

35680

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Motor proteins

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Vasopressin regulates renal water homeostasis via Aquaporins

Class of Gene

gene

gene_with_edited_transcript

(Rodriguez et al., 2012, Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-56

Cytogenetic map

43C7-43D1

Sequence location

2R:7,500,147..7,508,625 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

43C7-43D1

Limits computationally determined from genome sequence between P{lacW}cos^k16101 and P{EP}CG1600^EP398

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

43B-43B

(Maciver, 1996.9.17)

43C-43D

(Bonafe, 1997.5.12)

43B-43C

(determined by in situ hybridisation)

(MacIver et al., 1998, McCormack et al., 1996)

43C-43D

(determined by in situ hybridisation)

(Bonafe and Sellers, 1998)

Experimentally Determined Recombination Data

Location

2-56

(FlyBase, 2016)

2-56.6

(Comeron et al., 2012)

(Luschnig et al., 2004, McCormack et al., 1996)

Left of (cM)

(Krauss et al., 2009)

Right of (cM)

Notes

Maps to 2R.

(Luschnig et al., 2004)

Stocks and Reagents

Stocks (17)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

605271

w^*; P{UASp-didum.GT}3

14094

y¹; P{SUPor-P}didum^KG04384/CyO

605270

w^*; P{UASp-didum.Motor}3/TM3, Ser¹

605269

w^*; P{FRT(w^hs)}G13 didum⁸⁸

99723

w^*; P{UASp-didum.FL.EGFP}3/TM3, Ser¹

605268

w^*; P{FRT(w^hs)}G13 didum¹⁵⁴

99724

w^*; P{UAS-crb.wt}30.12e; P{UASp-didum.GT.EGFP}3

76986

y¹ sc^* v¹ sev²¹; P{TKO.GS00739}attP40

VDRC

v16902

w¹¹¹⁸; P{GD1848}v16902

v44291

w¹¹¹⁸; P{GD1848}v44291

v44292

w¹¹¹⁸; P{GD1848}v44292

v318855

PBac{fTRG01350.sfGFP-TVPTBF}VK00033

More stocks available...

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (57)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE30195

Other clones

FI23904

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0261397

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0261397

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Lu et al., 2018, Finan et al., 2011, Krauss et al., 2009, Toth et al., 2005)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

didum is required for wls protein-containing exosome release at synaptic boutons.

(Koles et al., 2012)

didum knockdown increases axonal mitochondrial length. didum acts to interrupt mitochondrial transport on microtubules to decrease the duty cycle and perhaps facilitate docking.

(Pathak et al., 2010)

didum protein associates with the osk mRNA transport complex preferentially at the oocyte cortex.

(Krauss et al., 2009)

One of 42 Drosophila genes identified as being most likely to reveal molecular and cellular mechanisms of nervous system development or plasticity relevant to human Mental Retardation disorders.

(Inlow and Restifo, 2004)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.

(Rogers et al., 2003)

Area matching Drosophila myosin V gene (inverted), Acc. No. AF003826.

(Davies, 2001.3.15)

Relationship to Other Genes

Source for database merge of

Source for merge of: soy didum

(Krauss et al., 2009)

Source for merge of: didum NEST:bs14c05

(FlyBase, 1996-)

Additional comments

soy mutants complement tud and stau mutants.

(Luschnig et al., 2004)

"l(2)43Cc" may correspond to "didum"; there is a band shift in the didum genomic region associated with the "l(2)43Cc⁴" mutation which is not a polymorphism, although it maps several kilobases 5' of the cloned genomic didum sequence.

(MacIver et al., 1998)

Source for merge of didum NEST:bs14c05 was sequence comparison ( date:031022 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: didum CG2146

(Gabrielian, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (25)

Reported As

Symbol Synonym

43CD

(Oliver et al., 1999)

CG2146

(National Institute of Genetics Fly Stocks, 2022-, Laffafian and Tepass, 2019, Kolkhof et al., 2017, Neuert et al., 2017, Çiçek et al., 2016, Harris et al., 2016, Hurd et al., 2016, Hosono et al., 2015, Rosa-Ferreira et al., 2015, Krahmer et al., 2013, Ramel et al., 2013, Petzoldt et al., 2012, Ni et al., 2010.12.1, Perkins et al., 2009, Rogers et al., 2003, Goldstein and Gunawardena, 2000)

Didum

(Miettinen and Björklund, 2015, Ramel et al., 2013, Zhang et al., 2013, Liebl and Featherstone, 2008, Bandyopadhyay et al., 2006)

DmV

(Toth et al., 2005, Yamashita et al., 2000)

(Finan et al., 2011)

MYOV

(Goldstein and Gunawardena, 2000)

Myo1C

(Kollmar, 2015.9.15)

Myo5

(Nishimura et al., 2021, Koles et al., 2012)

MyoV43CD

(Bonafe and Sellers, 1998)

NEST:bs14c05

NMC7

(MacIver et al., 1998)

didum

(Lee et al., 2024, Nowaskie et al., 2023, Doerflinger et al., 2022, Pang et al., 2021, Aguilar-Aragon et al., 2020, Lu et al., 2020, Lattner et al., 2019, Gene Disruption Project members, 2018-, Couderc et al., 2017, Neuert et al., 2017, Transgenic RNAi Project members, 2017-, Vazquez-Pianzola et al., 2017, Hurd et al., 2016, Sarov et al., 2016, Luck et al., 2014, Rudrapatna et al., 2014, Kwon et al., 2013, Kwon et al., 2013, Petzoldt et al., 2012, Rodriguez et al., 2012, Zoller and Schulz, 2012, Finan et al., 2011, Friedman et al., 2011, Dworkin et al., 2009, Krauss et al., 2009, Krauss et al., 2009, Haerty et al., 2007, Stuart et al., 2007, Texada et al., 2007, Wang and Lin, 2007, Franke et al., 2006, Liebl et al., 2006, Burgler and Macdonald, 2005, Liebl and Featherstone, 2005, Mermall et al., 2005, Nelson et al., 2005)

dmM5

(Ji et al., 2015)

myoV

(Massarwa et al., 2009)

soy

Name Synonyms

Didum

(Wong et al., 2011)

Myosin V

(Camelo and Luschnig, 2021, Voolstra and Huber, 2020, Schopf and Huber, 2017, Krahmer et al., 2013, Zoller and Schulz, 2012, Sun et al., 2011, Serio et al., 2010, Satoh et al., 2008, Rogers et al., 2003)

Myosin-V

(Bayer et al., 2024)

Myosin5

(Laffafian and Tepass, 2019)

MyosinV

(Shah et al., 2004)

dilute class unconventional myosin

(Rudrapatna et al., 2014, Wang and Lin, 2007, Nelson et al., 2005)

myosin V

(Couderc et al., 2017, Finan et al., 2011, Pathak et al., 2010, Nagy et al., 2009, Toth et al., 2005, Welte, 2004, Rogers et al., 2003, Bonafe et al., 2002, Goldstein and Gunawardena, 2000, Bonafe et al., 1999, Bonafe and Sellers, 1998, McCormack et al., 1998, Bonafe, 1997.5.12, Bonafe and Sellers, 1997)

myosin-5

(Ji et al., 2015)

myosin-V

(Milas and Telley, 2022, Bonafe et al., 1996)

shorty

(Krauss et al., 2009, Krauss et al., 2009, Luschnig et al., 2004, Luschnig et al., 2004)

Secondary FlyBase IDs

FBgn0069080
FBgn0015933
FBgn0023490
FBgn0045305

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 57 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35680

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A1Z6Z8

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A1Z6Z8

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35680/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0261397

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0261397

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG2146

MARRVEL_MODEL - MARRVEL (model organism gene)

35680

Linkouts

BioGRID - A database of protein and genetic interactions.

61556

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0261397

DroID - A comprehensive database of gene and protein interactions.

FBgn0261397

DRSC - Results frm RNAi screens

FBgn0261397

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

didum_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0015933

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0261397

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2146

Flygut - An atlas of the Drosophila adult midgut

FBgn0261397

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0261397

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC009935

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Motor proteins

MIST (protein-protein) - An integrated Molecular Interaction Database

35680

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Vasopressin regulates renal water homeostasis via Aquaporins

References (156)

The following external sites may use different assemblies or annotations than FlyBase.

1.81

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