FB2026_02 , released June 18, 2026
Gene: Dmel\Arp3
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General Information
Symbol
Dmel\Arp3
Species
D. melanogaster
Name
Actin-related protein 3
Annotation Symbol
CG7558
Feature Type
protein_coding_gene
FlyBase ID
FBgn0262716
Gene Model Status
Current
Stock Availability
15 publicly available
Gene Summary
Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks (By similarity). Seems to contact the pointed end of the daughter actin filament (By similarity). Required during embryogenesis for the developmental migration of tail hemocytes anteriorly, along the ventral midline (PubMed:25739458). (UniProt, P32392)
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

Arp66B, schwachling, Actr66B, Actin-related protein 66B

Key Links
Genomic Location
Cytogenetic map
66B6-66B6
Sequence location
Recombination map
3-22
RefSeq locus
NT_037436 REGION:8113407..8115902
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (21 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
contributes_to actin filament binding
inferred from biological aspect of ancestor with PANTHER:PTN000233796
(GO Reference Genome Project, 2011-)
enables structural constituent of cytoskeleton
inferred from sequence or structural similarity with UniProtKB:P61158
(Hudson and Cooley, 2002)
inferred from biological aspect of ancestor with PANTHER:PTN000940351
(GO Reference Genome Project, 2011-)
Biological Process (15 terms)
Terms Based on Experimental Evidence (14 terms)
CV Term
Evidence
References
involved_in actin cytoskeleton organization
inferred from physical interaction with FLYBASE:Arpc1; FB:FBgn0001961
(Stevenson et al., 2002)
inferred from mutant phenotype
(Richardson et al., 2007)
involved_in axonal fasciculation
inferred from mutant phenotype
(Zallen et al., 2002)
involved_in chaeta development
inferred from mutant phenotype
(Rajan et al., 2009)
involved_in intracellular protein transport
inferred from mutant phenotype
(Rajan et al., 2009)
involved_in lamellipodium assembly
inferred from mutant phenotype
(Rötte et al., 2024)
involved_in myoblast fusion
inferred from mutant phenotype
(Berger et al., 2008, Richardson et al., 2007)
inferred from genetic interaction with FLYBASE:WASp; FB:FBgn0024273
(Berger et al., 2008)
involved_in nuclear envelope budding
inferred from mutant phenotype
(Verboon et al., 2020)
involved_in plasma membrane tubulation
inferred from mutant phenotype
(Yan et al., 2013)
involved_in positive regulation of filopodium assembly
inferred from mutant phenotype
(Gonçalves-Pimentel et al., 2011)
involved_in positive regulation of lamellipodium assembly
inferred from mutant phenotype
(Georgiou and Baum, 2010)
involved_in positive regulation of synaptic assembly at neuromuscular junction
inferred from mutant phenotype
(Koch et al., 2014)
involved_in pseudocleavage involved in syncytial blastoderm formation
inferred from physical interaction with FLYBASE:Arpc1; FB:FBgn0001961
(Stevenson et al., 2002)
involved_in terminal button organization
inferred from mutant phenotype
(Koch et al., 2014)
involved_in wound healing, spreading of epidermal cells
inferred from mutant phenotype
(Rötte et al., 2024)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in Arp2/3 complex-mediated actin nucleation
inferred from biological aspect of ancestor with PANTHER:PTN000233796
(GO Reference Genome Project, 2011-)
inferred from sequence or structural similarity with UniProtKB:P61158
(Hudson and Cooley, 2002)
Cellular Component (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
located_in cytosol
inferred from direct assay
(Verboon et al., 2020)
located_in lamellipodium
inferred from direct assay
(Iwasa and Mullins, 2007)
located_in nuclear lamina
inferred from direct assay
(Verboon et al., 2020)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
part_of Arp2/3 protein complex
non-traceable author statement
(Vartiainen and Machesky, 2004)
inferred from biological aspect of ancestor with PANTHER:PTN000233796
(GO Reference Genome Project, 2011-)
inferred from sequence or structural similarity with UniProtKB:P61158
(Hudson and Cooley, 2002)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the actin family. ARP3 subfamily. (P32392)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
ACTIN-RELATED PROTEIN 2/3 COMPLEX ARPC3A VARIANT -
The Actin-related protein 2/3 complex is involved in the organization of the actin cytoskeleton, in particular, the nucleation of branched actin filaments. (Adapted from FBrf0144947).
ACTIN-RELATED PROTEINS -
Actin-related proteins (Arp) share significant sequence identity with actins. Arps broadly fall into two groups: nuclear Arps, involved in chromatin remodeling; and cytoplasmic Arps, associated with actin. (Adapted from PMID:21859859).
ACTIN-RELATED PROTEIN 2/3 COMPLEX ARPC3B VARIANT -
The Actin-related protein 2/3 complex is involved in the organization of the actin cytoskeleton, in particular, the nucleation of branched actin filaments. (Adapted from FBrf0144947).
Protein Function (UniProtKB)
Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks (By similarity). Seems to contact the pointed end of the daughter actin filament (By similarity). Required during embryogenesis for the developmental migration of tail hemocytes anteriorly, along the ventral midline (PubMed:25739458).
(UniProt, P32392)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Arp3 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry P32392)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Arp3-RA
FBtr0076737
NM_079244
1983
418
Arp3-RB
FBtr0334087
NM_001274630
1585
418
Additional Transcript Data and Comments
Reported size (kB)

1.257 (longest cDNA)

(Fyrberg et al., 1994)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Arp3-PA
FBpp0076460
47.0
418
5.84
P32392
NP_523968
AAF50488
Arp3-PB
FBpp0306212
47.0
418
5.84
P32392
NP_001261559
AGB94254
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

418 aa isoforms: Arp3-PA, Arp3-PB
Additional Polypeptide Data and Comments
Reported size (kDa)

418 (aa)

(Fyrberg et al., 1994)
Comments
External Data
Subunit Structure (UniProtKB)

Component of the Arp2/3 complex.

(UniProt, P32392)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Arp3 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.37

Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
distribution deduced from reporter or direct label
Stage
Tissue/Position (including subcellular localization)
Reference
oogenesis stage S9
nurse cell ring canal
(Hudson and Cooley, 2002)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data

Arp3 protein distribution was followed with an Arp3-GFP fusion protein. The GFP fusion protein is abundant in the cytoplasm of germ cells and is enriched in ring canals. Some enrichment at cortical membranes was also observed.

(Hudson and Cooley, 2002)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytosol
inferred from direct assay
(Verboon et al., 2020)
located_in lamellipodium
inferred from direct assay
(Iwasa and Mullins, 2007)
located_in nuclear lamina
inferred from direct assay
(Verboon et al., 2020)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Arp3 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 8 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Arp3
Transgenic constructs containing regulatory region of Arp3
Aberrations (Deficiencies and Duplications) ( 8 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal
lethal (with Arp383F)
lethal (with Arp3515FC)
lethal (with Arp31066PC)
lethal (with Arp3EP3640)
lethal - all die before end of P-stage | recessive
partially lethal - majority die, with Scer\GAL4pnr-MD237
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4tin.CΔ4
Other Phenotypes
Allele
abnormal cell migration, with Scer\GAL4Pxn.PS
abnormal locomotor behavior | third instar larval stage, with Scer\GAL4Ubi.PU
abnormal neuroanatomy, with Scer\GAL4C57
abnormal neuroanatomy | larval stage, with Scer\GAL4Ubi.PU
abnormal neuroanatomy | larval stage, with Scer\GAL4VGlut1-OK371
abnormal wound healing | P-stage, with Scer\GAL4A58
short lived | heat sensitive, with Scer\GAL4tin.CΔ4
some die during pharate adult stage | recessive
some die during pupal stage, with Scer\GAL4pnr-MD237
visible, with Scer\GAL4pnr-MD237
Phenotype manifest in
Allele
anterior dorsocentral bristle | pharate adult stage
bouton, with Scer\GAL4C57
cell projection, with Scer\GAL4Pxn.PS
dorsal pharyngeal muscle cell
embryonic/larval hemocyte, with Scer\GAL4Pxn.PS
embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4Ubi.PU
embryonic myoblast
external sensory organ precursor cell IIa | somatic clone
external sensory organ precursor cell IIb | somatic clone
female germline cyst | somatic clone
filamentous actin | embryonic stage, with Scer\GAL4VP16.mat.αTub67C
filopodium
fusion competent cell
lamellipodium | P-stage, with Scer\GAL4A58
larval somatic muscle cell
larval somatic muscle cell (with Arp3EP3640)
larval somatic muscle cell (with Arp3schwachling)
larval ventral nerve cord commissure (with Df(3L)pbl-X1)
macrochaeta | pharate adult stage
macrochaeta | somatic clone
mechanosensory chaeta | somatic clone
microchaeta | somatic clone
myoblast | embryonic stage
myoblast | embryonic stage (with Arp3EP3640)
myoblast | embryonic stage (with Arp3schwachling)
myoblast | embryonic stage (with Df(3L)ZP1)
neuromuscular junction, with Scer\GAL4C57
nurse cell & actin filament | germ-line clone
nurse cell | germline clone
nurse cell ring canal | germline clone
oocyte | germline clone
plasma membrane | embryonic stage
plasma membrane | embryonic stage, with Scer\GAL4VP16.mat.αTub67C
stalk follicle cell | somatic clone
synapse, with Scer\GAL4C57
trichogen cell, with Scer\GAL4pnr-MD237
type Ib terminal bouton | larval stage, with Scer\GAL4Ubi.PU
type Ib terminal bouton | larval stage, with Scer\GAL4VGlut1-OK371
vacuole | increased number, with Scer\GAL4Pxn.PS
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (41)
Hsap\ACTR3
14 of 14
Yes
Yes
1  
Hsap\ACTR3B
12 of 14
No
Yes
1  
Hsap\ACTA1
3 of 14
No
No
Hsap\ACTBL2
3 of 14
No
No
Hsap\ACTC1
3 of 14
No
No
1  
Hsap\ACTR1A
3 of 14
No
No
1  
Hsap\ACTR1B
3 of 14
No
No
1  
Hsap\ACTR2
3 of 14
No
No
Hsap\POTEE
3 of 14
No
No
Hsap\POTEI
3 of 14
No
No
Hsap\POTEJ
3 of 14
No
No
Hsap\ACTA2
2 of 14
No
No
Hsap\ACTB
2 of 14
No
No
5  
Hsap\ACTG1
2 of 14
No
No
1  
Hsap\ACTG2
2 of 14
No
No
Hsap\ACTL6A
2 of 14
No
No
2  
Hsap\ACTL6B
2 of 14
No
No
1  
Hsap\ACTL7A
2 of 14
No
No
Hsap\ACTL8
2 of 14
No
No
Hsap\ACTL9
2 of 14
No
No
Hsap\ACTR3C
2 of 14
No
Yes
Hsap\ACTR10
2 of 14
No
No
Hsap\ACTRT1
2 of 14
No
No
Hsap\ACTRT2
2 of 14
No
No
Hsap\ACTRT3
2 of 14
No
No
Hsap\POTEF
2 of 14
No
No
Hsap\ACTL7B
1 of 14
No
No
Hsap\ACTL10
1 of 14
No
No
Hsap\ACTR6
1 of 14
No
No
1  
Hsap\ACTR8
1 of 14
No
No
Hsap\LOC100288966
1 of 14
No
No
Hsap\LOC102723502
1 of 14
No
No
Hsap\POTEB2
1 of 14
No
No
Hsap\POTEB3
1 of 14
No
No
Hsap\POTEB
1 of 14
No
No
Hsap\POTEC
1 of 14
No
No
Hsap\POTED
1 of 14
No
No
Hsap\POTEG
1 of 14
No
No
Hsap\POTEH
1 of 14
No
No
Hsap\POTEKP
1 of 14
No
No
Hsap\POTEM
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (30)
Rnor\Actr3
13 of 14
Yes
Yes
Rnor\Actr3b
9 of 14
No
Yes
Rnor\Acta1
3 of 14
No
No
Rnor\Actbl2
3 of 14
No
No
Rnor\Actb
3 of 14
No
No
Rnor\Actc1
3 of 14
No
No
Rnor\Actg2
3 of 14
No
No
Rnor\Actr1a
3 of 14
No
No
Rnor\Actr1b
3 of 14
No
No
Rnor\Actr2
3 of 14
No
No
Rnor\Acta2
2 of 14
No
No
Rnor\Actg1
2 of 14
No
No
Rnor\Actl6a
2 of 14
No
No
Rnor\Actl6b
2 of 14
No
No
Rnor\Actl9b
2 of 14
No
No
Rnor\Actl9
2 of 14
No
No
Rnor\Actl10
2 of 14
No
No
Rnor\Actr10
2 of 14
No
No
Rnor\Actrt1
2 of 14
No
No
Rnor\Actrt3
2 of 14
No
No
Rnor\LOC100361457
2 of 14
No
No
Rnor\Actl7a
1 of 14
No
No
Rnor\Actl7b
1 of 14
No
No
Rnor\Actl11
1 of 14
No
No
Rnor\Actr6
1 of 14
No
No
Rnor\Actr8
1 of 14
No
No
Rnor\Actrt2
1 of 14
No
No
Rnor\LOC302827
1 of 14
No
Yes
Rnor\LOC102552318
1 of 14
No
No
Rnor\Potef
1 of 14
No
No
Mus musculus (laboratory mouse) (27)
Mmus\Actr3
13 of 14
Yes
Yes
Mmus\Actr3b
12 of 14
No
Yes
Mmus\Acta1
3 of 14
No
No
Mmus\Acta2
3 of 14
No
No
Mmus\Actbl2
3 of 14
No
No
Mmus\Actb
3 of 14
No
No
Mmus\Actc1
3 of 14
No
No
Mmus\Actg1
3 of 14
No
No
Mmus\Actg2
3 of 14
No
No
Mmus\Actr1a
3 of 14
No
No
Mmus\Actr1b
3 of 14
No
No
Mmus\Actr2
3 of 14
No
No
Mmus\Acte1
2 of 14
No
No
Mmus\Actl6a
2 of 14
No
No
Mmus\Actl6b
2 of 14
No
No
Mmus\Actl9
2 of 14
No
No
Mmus\Actl10
2 of 14
No
No
Mmus\Actr10
2 of 14
No
No
Mmus\Actrt1
2 of 14
No
No
Mmus\Actrt3
2 of 14
No
No
Mmus\Actl7a
1 of 14
No
No
Mmus\Actl7b
1 of 14
No
No
Mmus\Actl11
1 of 14
No
No
Mmus\Actr6
1 of 14
No
No
Mmus\Actr8
1 of 14
No
No
Mmus\Actrt2
1 of 14
No
No
Mmus\Ankrd36
1 of 14
No
Yes
Xenopus tropicalis (Western clawed frog) (16)
Xtro\actr3
8 of 13
Yes
Yes
Xtro\acta1
2 of 13
No
No
Xtro\acta4
2 of 13
No
No
Xtro\actb
2 of 13
No
No
Xtro\actc1
2 of 13
No
No
Xtro\actg1
2 of 13
No
No
Xtro\actr2
2 of 13
No
No
Xtro\acta2
1 of 13
No
No
Xtro\actl6a
1 of 13
No
No
Xtro\actr1a
1 of 13
No
No
Xtro\actr6
1 of 13
No
No
Xtro\actrt2
1 of 13
No
No
Xtro\klhl32
1 of 13
No
No
Xtro\men1
1 of 13
No
No
Xtro\MGC75582
1 of 13
No
No
Xtro\prnp
1 of 13
No
Yes
Danio rerio (Zebrafish) (21)
Drer\actr3b
12 of 14
Yes
Yes
Drer\actr3
7 of 14
No
Yes
Drer\acta1a
3 of 14
No
No
Drer\acta1b
3 of 14
No
No
Drer\actb1
3 of 14
No
No
Drer\actb2
3 of 14
No
No
Drer\actc1a
3 of 14
No
No
Drer\actc2
3 of 14
No
No
Drer\acte1
3 of 14
No
No
Drer\actr1b
3 of 14
No
No
Drer\actr2a
3 of 14
No
No
Drer\actr2b
3 of 14
No
No
Drer\acta2
2 of 14
No
No
Drer\actc1b
2 of 14
No
No
Drer\actc1c
2 of 14
No
Yes
Drer\actl6a
2 of 14
No
No
Drer\actl6b
2 of 14
No
No
Drer\actr10
2 of 14
No
No
Drer\zgc:101810
2 of 14
No
No
Drer\actr6
1 of 14
No
No
Drer\si:ch211-241j12.3
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (12)
Cele\arx-1
14 of 14
Yes
Yes
Cele\act-1
3 of 14
No
No
Cele\act-2
3 of 14
No
No
Cele\act-3
3 of 14
No
No
Cele\act-5
3 of 14
No
No
Cele\arx-2
3 of 14
No
No
Cele\act-4
2 of 14
No
No
Cele\arp-1
2 of 14
No
No
Cele\arp-11
2 of 14
No
No
Cele\F42C5.9
2 of 14
No
No
Cele\swsn-6
2 of 14
No
No
Cele\arp-6
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (13)
Agam\AgaP_AGAP005110
12 of 12
Yes
Yes
Agam\AgaP_AGAP001676
3 of 12
No
No
Agam\AgaP_AGAP002127
3 of 12
No
No
Agam\AgaP_AGAP005095
3 of 12
No
No
Agam\AgaP_AGAP011514
3 of 12
No
No
Agam\AgaP_AGAP011515
3 of 12
No
No
Agam\AgaP_AGAP011516
3 of 12
No
No
Agam\ACT5C_ANOGA
2 of 12
No
No
Agam\AgaP_AGAP000985
2 of 12
No
No
Agam\AgaP_AGAP008687
2 of 12
No
No
Agam\AgaP_AGAP012077
2 of 12
No
No
Agam\AgaP_AGAP005857
1 of 12
No
No
Agam\AgaP_AGAP010671
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (19)
Atha\DIS1
12 of 13
Yes
Yes
Atha\ACT1
3 of 13
No
No
Atha\ACT2
3 of 13
No
No
Atha\ACT3
3 of 13
No
No
Atha\ACT4
3 of 13
No
No
Atha\ACT7
3 of 13
No
No
Atha\ACT8
3 of 13
No
No
Atha\ACT9
3 of 13
No
No
Atha\ACT11
3 of 13
No
No
Atha\ACT12
3 of 13
No
No
Atha\AT2G42100
3 of 13
No
No
Atha\AT2G42170
3 of 13
No
No
Atha\ARP2
2 of 13
No
No
Atha\ARP4
2 of 13
No
No
Atha\ARP6
2 of 13
No
No
Atha\ARP7
2 of 13
No
No
Atha\ARP4A
1 of 13
No
No
Atha\ARP8
1 of 13
No
No
Atha\ARP9
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (8)
Scer\ARP3
12 of 13
Yes
Yes
Scer\ACT1
2 of 13
No
No
0  
Scer\ARP1
2 of 13
No
No
Scer\ARP2
1 of 13
No
No
Scer\ARP4
1 of 13
No
No
Scer\ARP7
1 of 13
No
No
Scer\ARP8
1 of 13
No
No
Scer\ARP9
1 of 13
No
No
Schizosaccharomyces pombe (Fission yeast) (8)
Spom\arp3
12 of 12
Yes
Yes
Spom\act1
2 of 12
No
No
Spom\alp5
1 of 12
No
No
Spom\arp1
1 of 12
No
No
Spom\arp2
1 of 12
No
No
Spom\arp8
1 of 12
No
No
Spom\arp9
1 of 12
No
No
Spom\arp42
1 of 12
No
No
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Arp3. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (14)
Act5C
5 of 13
Act42A
5 of 13
Act57B
5 of 13
Act79B
5 of 13
Act87E
5 of 13
Act88F
5 of 13
Arp1
4 of 13
Arp2
4 of 13
Arp10
4 of 13
Arp53D
4 of 13
Bap55
4 of 13
Arp6
3 of 13
Arp8
3 of 13
Arp5
2 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 2 )
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    ACTR3; actin related protein 3
    14 of 14
    604222
         
        ACTR3B; actin related protein 3B
        12 of 14
             
            ACTA1; actin alpha 1, skeletal muscle
            3 of 14
            102610
            ACTBL2; actin beta like 2
            3 of 14
            614835
                ACTC1; actin alpha cardiac muscle 1
                3 of 14
                ACTIN, ALPHA, CARDIAC MUSCLE; ACTC1
                 
                ACTR1A; actin related protein 1A
                3 of 14
                605143
                     
                    ACTR1B; actin related protein 1B
                    3 of 14
                    605144
                         
                        ACTR2; actin related protein 2
                        3 of 14
                        604221
                            POTEE; POTE ankyrin domain family member E
                            3 of 14
                            608914
                                POTEI; POTE ankyrin domain family member I
                                3 of 14
                                    POTEJ; POTE ankyrin domain family member J
                                    3 of 14
                                        Functional Complementation Data
                                        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
                                        Interactions
                                        Summary of Physical Interactions
                                        Summary of Genetic Interactions
                                        Interaction Browsers
                                        View in FlyBase Interactions Browser   View in MIST tool (external link)
                                        Please look at the allele data for full details of the genetic interactions
                                        Starting gene(s)
                                        Interaction type
                                        Interacting gene(s)
                                        Reference
                                        Arp3
                                        enhanceable
                                        Cip4
                                        (Nahm et al., 2010)
                                        Arp3
                                        enhanceable
                                        WASp
                                        (Berger et al., 2008)
                                        Starting gene(s)
                                        Interaction type
                                        Interacting gene(s)
                                        Reference
                                        arm
                                        suppressible
                                        Arp3
                                        (Sarpal et al., 2012)
                                        cindr
                                        suppressible
                                        Arp3
                                        (Johnson et al., 2008)
                                        Cip4
                                        enhanceable
                                        Arp3
                                        (Nahm et al., 2010)
                                        crb
                                        suppressible
                                        Arp3
                                        (Flores-Benitez and Knust, 2015)
                                        dock
                                        suppressible
                                        Arp3
                                        (Stark et al., 2021)
                                        Dora
                                        suppressible
                                        Arp3
                                        (Molina-Pelayo et al., 2022)
                                        WASp
                                        enhanceable
                                        Arp3
                                        (Berger et al., 2008)
                                        α-Cat
                                        suppressible
                                        Arp3
                                        (Sarpal et al., 2012)
                                        External Data
                                        Subunit Structure (UniProtKB)
                                        Component of the Arp2/3 complex.
                                        (UniProt, P32392 )
                                        Linkouts
                                        DroID - A comprehensive database of gene and protein interactions.
                                        MIST (genetic) - An integrated Molecular Interaction Database
                                        MIST (protein-protein) - An integrated Molecular Interaction Database
                                        Pathways
                                        Signaling Pathways (FlyBase)
                                        Metabolic Pathways
                                        FlyBase
                                        External Links
                                        External Data
                                        Linkouts
                                        KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                                        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                                        SignaLink - A signaling pathway resource with multi-layered regulatory networks.
                                        Class of Gene
                                        Genomic Location and Detailed Mapping Data
                                        Chromosome (arm)
                                        3L
                                        Recombination map
                                        3-22
                                        Cytogenetic map
                                        66B6-66B6
                                        Sequence location
                                        FlyBase Computed Cytological Location
                                        Cytogenetic map
                                        Evidence for location
                                        66B6-66B6
                                        Limits computationally determined from genome sequence between P{PZ}l(3)0721708223&P{EP}Rac2EP3118 and P{lacW}Nmtj1C7
                                        Experimentally Determined Cytological Location
                                        Cytogenetic map
                                        Notes
                                        References
                                        66B-66B
                                        (Fyrberg, 1993.5.4)
                                        66B-66B
                                        (determined by in situ hybridisation)
                                        (Fyrberg et al., 1994, Fyrberg and Fyrberg, 1993)
                                        Experimentally Determined Recombination Data
                                        Location

                                        3-22

                                        (FlyBase, 2016)

                                        3-19.0

                                        (Comeron et al., 2012)
                                        Left of (cM)
                                        Right of (cM)
                                        Notes
                                        Stocks and Reagents
                                        Stocks (15)
                                        Genomic Clones (17)
                                         

                                        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

                                        cDNA Clones (155)
                                         

                                        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

                                        cDNA clones, fully sequenced
                                        BDGP DGC clones
                                        Other clones
                                        Drosophila Genomics Resource Center cDNA clones

                                        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                                        cDNA Clones, End Sequenced (ESTs)
                                        BDGP DGC clones
                                        Other clones
                                        RNAi and Array Information
                                        Linkouts
                                        Antibody Information
                                        Laboratory Generated Antibodies
                                         
                                        Commercially Available Antibodies
                                         
                                        Cell Line Information
                                        Publicly Available Cell Lines
                                         
                                          Other Stable Cell Lines
                                           
                                            Other Comments

                                            S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

                                            (Stroschein-Stevenson et al., 2006)

                                            dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

                                            (Kiger et al., 2003)

                                            The Arp66B protein part of the Arp2/3 complex.

                                            (Stevenson et al., 2002)

                                            The Arp2/3 complex (including Arp66B) is required for actin cap expansion and pseudocleavage furrow formation during syncytial blastoderm divisions.

                                            (Stevenson et al., 2002)

                                            Isolation and characterisation of actin-related proteins Arp14D, Arp53D, Arp66B and Arp87C.

                                            (Fyrberg et al., 1994)

                                            Isolation and partial characterisation of Arp66B.

                                            (Fyrberg and Fyrberg, 1993)
                                            Relationship to Other Genes
                                            Source for database merge of

                                            Source for merge of: Arp66B schwachling

                                            (Berger et al., 2008)
                                            Additional comments
                                            Nomenclature History
                                            Source for database identify of

                                            Source for identity of: Arp3 Arp66B

                                            (FlyBase, 2009-)
                                            Nomenclature comments

                                            'Arp66B' renamed to 'Arp3' to rationalize the nomenclature of Actin-related protein (Arp) genes (by orthology with the mammalian and yeast genes) and better reflect community usage.

                                            (FlyBase, 2009-)
                                            Etymology
                                            Synonyms and Secondary IDs (16)
                                            Reported As
                                            Symbol Synonym
                                            ARP3
                                            (Zakrzewski et al., 2021, Reed et al., 2012, Goldstein and Gunawardena, 2000)
                                            AcTr66B
                                            Actr66B
                                            (Fyrberg and Fyrberg, 1993)
                                            Arp
                                            (Rout et al., 2022)
                                            Arp3
                                            (Mitra et al., 2026, Almeida Machado Costa et al., 2025, Sanhueza et al., 2025, Berry et al., 2024, Chen et al., 2024, De Groef et al., 2024, Lee et al., 2024, Li et al., 2024, Parkhurst, 2024.9.4, Pinot and Le Borgne, 2024, Tam and Harris, 2024, Biton et al., 2023, Cazzagon et al., 2023, Fernandez-Gonzalez and Harris, 2023, Hui et al., 2023, Lehne and Bogdan, 2023, Molina-Pelayo et al., 2022, Papagiannouli, 2022, Zhou et al., 2022, Bischoff and Bogdan, 2021, Chaudhari et al., 2021, Hirschhäuser et al., 2021, Johnson, 2021, Kilo et al., 2021, Loganathan et al., 2021, Martin et al., 2021, Sarkar et al., 2021, Xie et al., 2021, Chougule et al., 2020, Davidson and Wood, 2020, Deng et al., 2020, Dubey et al., 2020, Earl et al., 2020, Ko and Martin, 2020, López-Gay et al., 2020, Lv et al., 2020, Meltzer and Schuldiner, 2020.6.25, Schroeder et al., 2020, Thestrup et al., 2020, Verboon et al., 2020, Wang et al., 2020, Blake-Hedges and Megraw, 2019, Dialynas et al., 2019, Gao et al., 2019, Hurst et al., 2019, Jiang and Harris, 2019, Jiang et al., 2019, Lee and Chen, 2019, Trylinski and Schweisguth, 2019, Tsarouhas et al., 2019, Caridi et al., 2018, Salazar and Yamamoto, 2018, Segal et al., 2018, Flores-Benitez and Knust, 2015, Hsiao et al., 2015, Kollmar, 2015.9.15, Levayer et al., 2015, Rodal et al., 2015, Rosa et al., 2015, Verboon et al., 2015, Haralalka et al., 2014, Koch et al., 2014, Ingerman et al., 2013, Leibfried et al., 2013, Yan et al., 2013, Abmayr and Pavlath, 2012, Giagtzoglou et al., 2012, Reed et al., 2012, Sarpal et al., 2012, Zoller and Schulz, 2012, Isaji et al., 2011, Rotkopf et al., 2011, Eriksson et al., 2010, Serio et al., 2010, Massarwa et al., 2009, Rajan et al., 2009, Berger et al., 2008, Richardson and Baylies, 2008, Schäfer et al., 2008, Iwasa and Mullins, 2007, Jovceva et al., 2007, Rajan et al., 2007, Richardson et al., 2007, Kollmar and Odronitz, 2006, Price et al., 2006, Frank et al., 2005, Noguchi et al., 2005, Beltzner and Pollard, 2004, Biyasheva et al., 2004, Bogdan et al., 2004, Noguchi et al., 2004, Vartiainen and Machesky, 2004, Grevengoed et al., 2003, Lundquist, 2003, Rogers et al., 2003, Hudson and Cooley, 2002, Hudson and Cooley, 2002, Jacinto et al., 2002, Kiehart and Franke, 2002, Miller, 2002, Rogat and Miller, 2002, Rorth, 2002, Stevenson et al., 2002, Tal et al., 2002, Zallen et al., 2002, Hudson and Cooley, 2001)
                                            Arp66B
                                            (Das and Cagan, 2017, Gonzalez et al., 2014, Lee et al., 2014, Antunes et al., 2013, Kwon et al., 2013, Abmayr and Pavlath, 2012, Japanese National Institute of Genetics, 2012.5.21, Friedman et al., 2011, Gonçalves-Pimentel et al., 2011, Rohn et al., 2011, Rotkopf et al., 2011, D'Ambrosio and Vale, 2010, Berger et al., 2008, Bakal et al., 2007, Stuart et al., 2007, Stroschein-Stevenson et al., 2006)
                                            CG7558
                                            (Kolkhof et al., 2017, Hosono et al., 2015, Abmayr and Pavlath, 2012, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Goldstein and Gunawardena, 2000)
                                            anon-WO0172774.138
                                            arp3
                                            (Bourouliti and Skoulakis, 2022, Bischoff et al., 2021, Hu et al., 2021, Stark et al., 2021, Green et al., 2018, Zhang et al., 2018, Rudolf et al., 2014, Herszterg et al., 2013, Hatan et al., 2011, Hornbruch-Freitag et al., 2011, Nahm et al., 2010, Leibfried et al., 2008, Noguchi et al., 2008, Frank et al., 2006, Rogat and Miller, 2002, Frankel and Mooseker, 1996)
                                            arp66B
                                            (Sánchez-Soriano et al., 2010, Georgiou et al., 2008, Johnson et al., 2008, Wang et al., 2008, Fyrberg, 1993.5.4)
                                            arp66b
                                            (Georgiou and Baum, 2010)
                                            schwachling
                                            Name Synonyms
                                            Actin-related protein
                                            (Fyrberg and Fyrberg, 1993)
                                            Actin-related protein 3
                                            Actin-related protein 66B
                                            (Fyrberg et al., 1994)
                                            actin-related protein-3
                                            (Rajan et al., 2009)
                                            schwachling
                                            (Berger et al., 2008, Griemert et al., 2007)
                                            Secondary FlyBase IDs
                                            • FBgn0086714
                                            • FBgn0011744
                                            • FBgn0046458
                                            Datasets (0)
                                            Study focus (0)
                                            Experimental Role
                                            Project
                                            Project Type
                                            Title
                                            Study result (0)
                                            Result
                                            Result Type
                                            Title
                                            External Crossreferences and Linkouts ( 42 )
                                            Sequence Crossreferences
                                            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                                            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                                            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                                            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                                            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                                            UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
                                            Other crossreferences
                                            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                                            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                                            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                                            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                                            InterPro - A database of protein families, domains and functional sites
                                            KEGG Genes - Molecular building blocks of life in the genomic space.
                                            MARRVEL_MODEL - MARRVEL (model organism gene)
                                            Linkouts
                                            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                                            DroID - A comprehensive database of gene and protein interactions.
                                            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                                            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                                            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                                            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                                            FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
                                            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                                            KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                                            MIST (genetic) - An integrated Molecular Interaction Database
                                            MIST (protein-protein) - An integrated Molecular Interaction Database
                                            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                                            SignaLink - A signaling pathway resource with multi-layered regulatory networks.
                                            References (232)