FlyBase Gene Report: Dmel\Tlk

General Information

Symbol

Dmel\Tlk

Species

D. melanogaster

Name

Tousled-like kinase

Annotation Symbol

CG34412

Feature Type

protein_coding_gene

FlyBase ID

FBgn0283657

Gene Model Status

Current

Stock Availability

56 publicly available

Gene Summary

Tousled-like kinase (Tlk) encodes a serine/threonine kinase that acts in a cell cycle-dependent manner. It contributes to cell morphogenesis, cell migration and cell death through interactions with chromatin components. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

l(1)G0054, EP1413, BEST:GH17330 , BcDNA:GH07910

Key Links

Genomic Location

Cytogenetic map

3E6-3F1

Sequence location

X:3,720,007..3,789,770 [+]

Recombination map

1-4

RefSeq locus

NC_004354 REGION:3720007..3789770

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (11 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with FLYBASE:asf1; FB:FBgn0029094

(Carrera et al., 2003)

enables protein serine/threonine kinase activity

inferred from direct assay

(Carrera et al., 2003)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR017441

(InterPro Project Members, 2004-)

enables protein kinase activity

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR008271

(InterPro Project Members, 2004-)

Biological Process (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in chromatin organization

inferred from genetic interaction with FLYBASE:asf1; FB:FBgn0029094

(Carrera et al., 2003)

involved_in chromosome segregation

inferred from genetic interaction with FLYBASE:mars; FB:FBgn0033845

(Li et al., 2009)

involved_in post-translational protein modification

inferred from mutant phenotype

(Carrera et al., 2003)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in chromosome segregation

inferred from biological aspect of ancestor with PANTHER:PTN000540737

(GO Reference Genome Project, 2011-)

involved_in intracellular signal transduction

inferred from biological aspect of ancestor with PANTHER:PTN000540738

(GO Reference Genome Project, 2011-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in heterochromatin

inferred from direct assay

(Swenson et al., 2016)

located_in nucleoplasm

inferred from high throughput direct assay

(Lye et al., 2014)

located_in nucleus

inferred from direct assay

(Carrera et al., 2003)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

UNCLASSIFIED CONVENTIONAL PROTEIN KINASE DOMAIN KINASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

UNCLASSIFIED CONVENTIONAL PROTEIN KINASE DOMAIN KINASES -: This group comprises conventional protein kinase domain kinases that do not classify under other groups in FlyBase.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Tlk for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry B7Z126)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.55

3' UTR contains an ORF that is conserved among close Drosophila species (FBrf0202906; potential peptide 129 aa in length).

Gene model reviewed during 6.46

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Due to significant sequence change in this region between Releases 4 and 5, data that predate Release 5 are not shown for some categories of evidence.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Stop-codon suppression (UAG) postulated; FBrf0243886.

Gene model reviewed during 6.32

Double stop-codon suppression (UAG, UAA) postulated; FBrf0243886.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Tlk-RB

FBtr0112680

NM_001103403

4624

1463

Tlk-RC

FBtr0070596

NM_130717

5849

1254

Tlk-RE

FBtr0299579

NM_206620

5537

1255

Tlk-RF

FBtr0299580

NM_001144680

6513

1266

Tlk-RG

FBtr0299581

NM_001144681

7243

1489

Tlk-RH

FBtr0299582

NM_001144682

5890

1266

Tlk-RI

FBtr0299583

NM_001144683

4673

1242

Tlk-RJ

FBtr0301659

NM_001169184

4882

1266

Tlk-RK

FBtr0333751

NM_001272291

7403

1265

Tlk-RL

FBtr0333752

NM_001272292

4308

1011

Tlk-RM

FBtr0333753

NM_001272293

7490

1242

Tlk-RN

FBtr0345319

NM_001297914

4355

1242

Tlk-RO

FBtr0479803

NM_001382078

5890

1276

Tlk-RP

FBtr0481732

NM_001414088

5890

1412

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Tlk-PB

FBpp0111592

160.3

1463

9.10

FBpp0099673

135.5

1254

9.31

FBpp0288854

135.6

1255

9.36

FBpp0288855

136.7

1266

9.10

FBpp0288856

162.2

1489

9.62

FBpp0288857

136.7

1266

9.10

FBpp0288858

133.9

1242

9.02

FBpp0290873

136.7

1266

9.10

FBpp0305893

136.4

1265

9.25

FBpp0305894

110.0

1011

9.50

FBpp0305895

133.9

1242

9.02

FBpp0311479

133.9

1242

9.02

FBpp0428136

137.8

1276

9.24

QJC18358

Tlk-PP

FBpp0428486

152.7

1412

9.20

UYK33052

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1266 aa isoforms: Tlk-PF, Tlk-PH, Tlk-PJ

1242 aa isoforms: Tlk-PI, Tlk-PM, Tlk-PN

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Tlk using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.77

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 3 -- 9

organism | ubiquitous

(Carrera et al., 2003)

larval stage

imaginal disc

(Carrera et al., 2003)

adult stage | female & oogenesis stage S10

nurse cell & oocyte

Comment: maternal transcript transported from nurse cells to oocytes

(Carrera et al., 2003)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 3

(Carrera et al., 2003)

Additional Descriptive Data

tlk is ubiquituously distributed in all proliferative tissues.

(Carrera et al., 2003)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 5 -- 8

cell

• nucleus

Comment: interphase

(Carrera et al., 2003)

larval stage

salivary gland

• nucleus

Comment: interphase

(Carrera et al., 2003)

adult stage | female & oogenesis

follicle cell

(Xiang et al., 2016)

oogenesis & adult stage | female

nurse cell

(Xiang et al., 2016)

oocyte

(Xiang et al., 2016)

polar follicle cell | intense

(Xiang et al., 2016)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

fertilized egg stage

egg

(Carrera et al., 2003)

embryonic stage

organism

• nucleus

(Carrera et al., 2003)

Additional Descriptive Data

tlk protein is localized to the nucleus in interphase cells, and diffusely distributed throughout the cell during other parts of the mitotic cell cycle. tlk protein does not associate directly with DNA.

(Carrera et al., 2003)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in heterochromatin

inferred from direct assay

(Swenson et al., 2016)

located_in nucleoplasm

inferred from high throughput direct assay

(Lye et al., 2014)

located_in nucleus

inferred from direct assay

(Carrera et al., 2003)

Expression Deduced from Reporters

Reporter: P{GawB}Tlk^NP7281

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

lobula columnar neuron LC4

(Flybrain Neuron Database et al., 2018.6.28, Matsuo et al., 2016, Otsuna and Ito, 2006)

lobula | restricted

(Otsuna and Ito, 2006)

optic glomerulus of the PVLP

(Matsuo et al., 2016)

lobula plate-lobula columnar neuron LPLC

(Flybrain Neuron Database et al., 2018.6.28)

type 1 lobula plate-lobula neuron

(Flybrain Neuron Database et al., 2018.6.28)

complex columnar visual projection neuron CC1

(Flybrain Neuron Database et al., 2018.6.28)

Reporter: PBac{CPTI-un}Tlk^CPTI002768

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain cell body rind

(Knowles-Barley, 2011.8.24)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Tlk in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Tlk

View exonic expression by tissue for Dmel\Tlk

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0086899

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

318206/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0283657

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0086899

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0283657

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG32782

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 88 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 22 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Tlk

Transgenic constructs containing regulatory region of Tlk

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 4 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

cell lethal | somatic clone

Tlk^Δ14

lethal, with Scer\GAL4^pnr-MD237

Tlk^GD9828

lethal | germline clone | male limited

Tlk^G0054

lethal | larval stage

Tlk^G0054

lethal | larval stage | recessive

Tlk^G0054

lethal | prepupal stage

Tlk^G0054

lethal | pupal stage

Tlk^G0054

lethal | recessive

lethal - all die before end of larval stage | heat sensitive, with Scer\GAL4^hs.2sev

Tlk^UAS.cCa

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

lethal - all die during embryonic stage | recessive

Sterility

Allele

female sterile, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

Tlk^GL00200

sterile | female | germline clone

Other Phenotypes

Allele

abnormal mitotic cell cycle, with Scer\GAL4^GCN4.bcd3.1

Tlk^UAS.cCa

abnormal mitotic cell cycle, with Scer\GAL4^GMR.PF

Tlk^UAS.cCa

abnormal mitotic cell cycle | embryonic stage

Tlk^Δ14

abnormal size | adult stage, with Scer\GAL4^hs.2sev

Tlk^UAS.cCa

decreased body size | adult stage, with Scer\GAL4^Ilp2.PR

decreased cell number | larval stage, with Scer\GAL4^arm.PS

Tlk^UAS.cCa

decreased cell number | larval stage, with Scer\GAL4^Bx-MS1096

Tlk^UAS.cCa

decreased cell number | larval stage, with Scer\GAL4^hs.PH

Tlk^UAS.cCa

decreased fecundity | germline clone

increased cell death, with Scer\GAL4^arm.PS

Tlk^UAS.cCa

increased cell death, with Scer\GAL4^Bx-MS1096

Tlk^UAS.cCa

increased cell death, with Scer\GAL4^{VP16.mat.αTub67C}

Tlk^UAS.cCa

increased cell death | embryonic stage

Tlk^Δ14

increased cell size | larval stage, with Scer\GAL4^arm.PS

Tlk^UAS.cCa

increased cell size | larval stage, with Scer\GAL4^Bx-MS1096

Tlk^UAS.cCa

increased cell size | larval stage, with Scer\GAL4^hs.PH

Tlk^UAS.cCa

short lived, with Scer\GAL4^da.Switch.PT

Tlk^UAS.Tag:FLAG

short lived, with Scer\GAL4^Mhc.F3-580

Tlk^UAS.Tag:FLAG

some die during embryonic stage | recessive

some die during larval stage | heat sensitive, with Scer\GAL4^hs.2sev

Tlk^UAS.cCa

some die during pupal stage, with Scer\GAL4^pnr-MD237

suppressor of variegation, with P{SUPor-P}KV00108

Tlk^MI11233

visible, with Scer\GAL4^GMR.PF

Tlk^EP1413

visible, with Scer\GAL4^sca-537.4

Tlk^EP1408

Phenotype manifest in

Allele

embryonic/larval hemocyte | third instar larval stage, with Scer\GAL4^Pxn.PS

Tlk^EP1200

embryonic/larval salivary gland, with Scer\GAL4^hs.2sev

eye, with Scer\GAL4^ey.PH

eye, with Scer\GAL4^GMR.PF

Tlk^EP1413

head, with Scer\GAL4^ey.PH

Tlk^dead.UAS

larval salivary gland & nuclear chromosome, with Scer\GAL4^hs.2sev

Tlk^UAS.cCa

macrochaeta, with Scer\GAL4^hs.2sev

Tlk^UAS.cCa

tormogen cell | ectopic, with Scer\GAL4^sca-537.4

Tlk^EP1408

trichogen cell | ectopic, with Scer\GAL4^sca-537.4

Tlk^EP1408

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (15)

Hsap\TLK2

10 of 14

Yes

Hsap\TLK1

9 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (10)

Rnor\Tlk2

10 of 14

Yes

Rnor\Tlk1

8 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (16)

Mmus\Tlk2

10 of 14

Yes

Mmus\Tlk1

9 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Mark4

1 of 14

Mmus\Nuak1

1 of 14

Mmus\Pim2

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (16)

Xtro\tlk2

7 of 13

Yes

Xtro\rpl27a

5 of 13

Xtro\pcmtd1

4 of 13

Yes

Xtro\tlk1

4 of 13

Yes

Xtro\usp30

4 of 13

Xtro\aurka

1 of 13

Xtro\cnp

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (13)

Drer\tlk2

10 of 14

Yes

Drer\tlk1a

8 of 14

Yes

Drer\tlk1b

8 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (4)

Cele\tlk-1

11 of 14

Yes

Cele\jkk-1

1 of 14

Cele\par-1

1 of 14

Cele\unc-82

1 of 14

Anopheles gambiae (African malaria mosquito) (6)

Agam\AgaP_AGAP000043

9 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (17)

Atha\TSL

9 of 13

Yes

Atha\AT2G20470

1 of 13

Atha\AT2G37840

1 of 13

Atha\AT3G20200

1 of 13

Yes

Atha\AT3G23310

1 of 13

Atha\AT5G09890

1 of 13

Atha\AT5G57035

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\WAKL2

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (11)

Scer\BCK1

1 of 13

Yes

Scer\CDC5

1 of 13

Yes

Scer\HRK1

1 of 13

Yes

Scer\MPS1

1 of 13

Yes

Scer\NPR1

1 of 13

Yes

Scer\PRR2

1 of 13

Yes

Scer\RTK1

1 of 13

Yes

Scer\SCH9

1 of 13

Yes

Scer\SNF1

1 of 13

Yes

Scer\SSK2

1 of 13

Yes

Scer\TPK1

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (6)

Spom\ark1

1 of 12

Yes

Spom\mug27

1 of 12

Yes

Spom\orb6

1 of 12

Yes

Spom\plo1

1 of 12

Yes

Spom\ssp2

1 of 12

Yes

Spom\wis4

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Tlk. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (12)

2 of 13

1 of 13

1 of 13

1 of 13

for

1 of 13

Lk6

1 of 13

lok

1 of 13

Mekk1

1 of 13

Pak3

1 of 13

PhKγ

1 of 13

Tao

1 of 13

wts

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

TLK2; tousled like kinase 2

model of autosomal dominant intellectual developmental disorder 57

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Tlk^HMS00943

exacerbates cancer

modeled by Rbf^HMS03004

(Parkhitko et al., 2021)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TLK2; tousled like kinase 2

10 of 14

608439

INTELLECTUAL DEVELOPMENTAL DISORDER, AUTOSOMAL DOMINANT 57; MRD57

autosomal dominant intellectual developmental disorder 57

TLK1; tousled like kinase 1

9 of 14

608438

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Tlk - Cen

experimental knowledge based

(Guruharsha et al., 2011)

Tlk - Fgop2

experimental knowledge based

(Guruharsha et al., 2011)

Tlk - MED25

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

Tlk - Panx

experimental knowledge based

(Guruharsha et al., 2011)

Tlk - Psi

enzymatic study, peptide massfingerprinting, autoradiography

(Taliaferro et al., 2013)

Tlk - Slmap

experimental knowledge based

(Guruharsha et al., 2011)

Tlk - Tak1

anti bait coimmunoprecipitation, western blot

(Liaw and Chiang, 2019)

Tlk - Ubqn

experimental knowledge based

(Guruharsha et al., 2011)

Tlk - asf1

enzymatic study, autoradiography

(Pilyugin et al., 2009)

Tlk - cmet

experimental knowledge based

(Guruharsha et al., 2011)

Tlk - ctp

anti tag coimmunoprecipitation, Identification by mass spectrometry, anti tag western blot

(Schnabl et al., 2021)

Tlk - dup

experimental knowledge based

(Guruharsha et al., 2011)

Tlk - geminin

experimental knowledge based

(Guruharsha et al., 2011)

Tlk - nxf2

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Tlk

enhanceable

asf1

(Carrera et al., 2003)

Tlk

suppressible

asf1

(Carrera et al., 2003)

Tlk

enhanceable

mars

(Li et al., 2009)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

asf1

enhanceable

Tlk

(Carrera et al., 2003)

suppressible

(Zhu et al., 2005)

enhanceable

(Zhu et al., 2005)

suppressible

Tlk

(Abdelilah-Seyfried et al., 2000)

enhanceable

Tlk

(Müller et al., 2005)

enhanceable

(Li et al., 2009)

suppressible

(Li et al., 2009)

enhanceable

Tlk

(Abdelilah-Seyfried et al., 2000)

pbl

enhanceable

Tlk

(Gregory et al., 2007)

pnr

enhanceable

Tlk

(Pena-Rangel et al., 2002)

pnr

suppressible

Tlk

(Pena-Rangel et al., 2002)

suppressible

(Zhu et al., 2005)

enhanceable

(Zhu et al., 2005)

External Data

Linkouts

MIST (genetic) - An integrated Molecular Interaction Database

318206

MIST (protein-protein) - An integrated Molecular Interaction Database

318206

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

gene

gene_with_stop_codon_read_through

(FlyBase Genome Annotators, 2020, FlyBase Genome Annotators, 2019-)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-4

Cytogenetic map

3E6-3F1

Sequence location

X:3,720,007..3,789,770 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

3E6-3F1

Left limit from in situ hybridisation (FBrf0128367) Right limit from in situ hybridisation (FBrf0128367); Limits computationally determined from genome sequence between P{EP}EP1200^EP1200&P{EP}EP1343 and P{EP}CG2930^EP1352 3F1--5

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

3F4-3F5

(determined by in situ hybridisation)

(Peter et al., 2002, Schäfer et al., 1999.3.29)

3E6-3E7

(determined by in situ hybridisation)

(Abdelilah-Seyfried et al., 2000)

3F1-3F5

(determined by in situ hybridisation)

(Schäfer et al., 1999.6.15)

Experimentally Determined Recombination Data

Location

1-4

(FlyBase, 2016)

1-4.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (56)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

29962

w¹¹¹⁸; P{UAS-Tlk}3

FlyORF

F001746

M{UAS-Tlk.ORF}ZH-86Fb

F001261

M{UAS-Tlk.ORF.3xHA}ZH-86Fb

Korea

10638

P{EP}Tlk^G311

12243

P{VT056450-GAL4}attP2

15308

P{VT056448-GAL4}attP2

11264

11282

11323

11355

2829R-1

v105732

More stocks available...

Genomic Clones (26)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (101)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0283657

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Yeh et al., 2015)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes decreased γ-tubulin staining at the spindle pole and misalignment of chromosomes along the spindle when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

Annotation CG12462 eliminated in release 5.1 of the genome annotation.

(FlyBase Genome Annotators, 2006)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a greater than three-fold increase in AttA activity in response to heat-killed E.coli after ecdysone treatment in S2 cells.

(Kleino et al., 2005)

When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of S phase cells, G2/M+, an increase in the proportion of G2/M phase cells, a decrease in cytokinetic index and a decrease in the mitotic index are seen.

(Bettencourt-Dias et al., 2004)

New annotation (CG33219) in release 3.2 of the genome annotation.

(FlyBase Genome Annotators, 2004)

The release 4 CG32782 annotation contains sequences corresponding to release 2 annotation CG2829. The release 3 version of CG32782 was incomplete and was missing most of the sequence corresponding to release 2 annotation CG2829 (possibly due to an assembly error), but this has been fixed in release 4 and the release 4 CG32782 annotation now includes all of the release 2 CG2829 sequence. 'CG33219' does not appear in Release 4. The region it was located in in Release 3.2 is now a large TE. 'CG33219' is homologous to annotation CG32782; presumably that is where this little bit of mislaid 'CG33219' sequence originated.

(FlyBase Genome Annotators, 2004-2005)

The tlk-asf1 pathway is important for both DNA replication and chromatin assembly. tlk may coordinate cell cycle progression through the regulation of chromatin dynamics.

(Carrera et al., 2003)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R⁺ cells: cells become round and detached.

(Kiger et al., 2003)

New annotation (CG32782) in release 3 of the genome annotation. Annotation CG2829 was eliminated in release 3 of the genome annotation, October 2002.

(FlyBase Genome Annotators, 2002-2003)

New annotation (CG32781) in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Relationship to Other Genes

Source for database merge of

Source for merge of: tlk anon-WO03040301.114 anon-WO03040301.116

(FlyBase, 1996-)

Source for merge of: tlk CG32781

(FlyBase Genome Annotators, 2007)

Source for merge of: CG12462 EP1413

(Müller et al., 2005)

Source for merge of: CG32782 CG33219

(FlyBase Genome Annotators, 2004-2005)

Source for merge of: tlk CG12462

(FlyBase Genome Annotators, 2007)

Source for merge of: tlk CG32782

(FlyBase, 1996-)

Source for merge of: Tlk EP1408

(Abdelilah-Seyfried et al., 2000)

Additional comments

Annotations CG32782 and CG32781 merged as CG34412 in release 5.2 of the genome annotation.

(FlyBase Genome Annotators, 2007)

Sequences corresponding to annotation CG12462 (which was eliminated in release 5.1 of the genome annotation) have been reinstated as part of the CG34412 annotation (which corresponds to tlk) in release 5.2 of the genome annotation.

(FlyBase Genome Annotators, 2007)

"l(1)G0299" may affect "CG2829". "l(1)G0167" may affect "CG2829". "l(1)G0343" may affect "CG2829". "l(1)G0054" may affect "CG2829".

(Peter et al., 2002)

Source for merge of tlk anon-WO03040301.114 anon-WO03040301.116 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Source for merge of tlk CG32782 was sequence comparison ( date:050324 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: Tlk tlk

(FlyBase, 2009-)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (23)

Reported As

Symbol Synonym

BEST:GH17330

BcDNA:GH07910

(Peter et al., 2002)

CG12462

(Gregory et al., 2007, Gilfillan et al., 2006)

CG2829

(Zhu et al., 2005, Bettencourt-Dias et al., 2004, Kiger et al., 2003, Kiger et al., 2003, Brody et al., 2002, Morrison et al., 2000, Whitfield, 2000.5.10)

CG32781

(Gilfillan et al., 2006)

CG32782

(Schertel et al., 2013, Blanco et al., 2010, Hogan and Bettencourt, 2009, Goshima et al., 2007, Kleino et al., 2005)

CG33219

(FlyBase Genome Annotators, 2004-2005)

CG34412

(Hirschhäuser et al., 2021, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Ni et al., 2008, FlyBase Genome Annotators, 2007)

EP(X)1408

(Pena-Rangel et al., 2002)

EP(X)1413

(Pena-Rangel et al., 2002)

EP1408

EP1413

(Zhu et al., 2005)

GH17330

(Pena-Rangel et al., 2002)

TLK

(Gong and Cheng, 2026, Moshkin et al., 2009, Pilyugin et al., 2009)

anon-WO03040301.114

anon-WO03040301.116

dtlk

(Chou and Liaw, 2006)

l(1)G0054

(Peter et al., 2002, Schäfer et al., 1999.3.29, Schäfer et al., 1999.6.15)

Name Synonyms

BEST:GH17330

CG12462

CG32781

Tousled like kinase

(Pogson et al., 2014)

Tousled-like kinase

(Merdes, 2010.2.24, Merdes, 2010.8.5, Hogan and Bettencourt, 2009, Carrera et al., 2003)

tousled-like kinase

(Zhang et al., 2016, Taliaferro et al., 2013, Li et al., 2009, Stofanko et al., 2008)

Secondary FlyBase IDs

FBgn0086899
FBgn0052781
FBan0032782
FBgn0085441
FBgn0053219
FBgn0052782
FBgn0066856
FBgn0066855
FBgn0028489
FBgn0026698
FBgn0086709
FBgn0062688
FBgn0029675
FBgn0043460

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 79 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

318206

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

B7Z126

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

B7Z126

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0086899

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

318206/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0283657

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0283657

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG34412

MARRVEL_MODEL - MARRVEL (model organism gene)

318206

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0283657

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0086899

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0283657

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG32782

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC031766

MIST (genetic) - An integrated Molecular Interaction Database

318206

MIST (protein-protein) - An integrated Molecular Interaction Database

318206

References (145)

The following external sites may use different assemblies or annotations than FlyBase.

-0.77

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