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FB2026_01
,
released March 12, 2026
Cg25C, DCg1, collagen IV, Collagen type IV, ColIVα1
along with Collagen IV, the main component of basement membranes - synthesized by the fat body, secreted to the hemolymph, and incorporated into the basement membrane - determines organ shape - regulates BMP signaling
Please see the JBrowse view of Dmel\Col4a1 for information on other features
To submit a correction to a gene model please use the Contact FlyBase form
AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.46
6.3 (northern blot)
1775 (aa)
Trimers of two alpha 1(IV) and one alpha 2(IV) chain. Type IV collagen forms a mesh-like network linked through intermolecular interactions between 7S domains and between NC1 domains.
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
Alpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Col4a1 using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: reported as plasmatocytes anlage
Col4a1 expression initiates later in embryogenesis than LanA and shows a different development profile. Col4a1 transcripts are first detected in 6-8hr embryos and reach a plateau by 16-18hr. Levels remain high through the larval instars, decrease in late third instar, and increase throughout the last two hours before eclosion.
Detected in embryonic, larval, pupal and adult RNA.
Protein is evenly distributed on distal cell surfaces throughout the leg at 18 h after pupation. At 30 h after pupation protein is concentrated on the basal surface of distal joint cells but is much weaker in other leg regions.
JBrowse - Visual display of RNA-Seq signals
View Dmel\Col4a1 in JBrowse
2-15
2-16.5
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
monoclonal
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Homologous genetic loci in D.subobscura and D.melanogaster tend to show a similar ultrastructure in the two species.
Decrease in Cg25C expression causes defective muscle attachments. Mutant phenotypes suggest that type IV collagen acts to stabilise call-matrix interactions.
Cg25C is a member of the haemocyte and fat body enhancer detector strains.
Cg25C serves as a terminal fat cell differentiation marker.
The carboxy terminal fragment of the Cg25C protein, termed gp125, is identical with a collagenous polypeptide that appears during metamorphosis.
Changes in expression of LanA precede those of Cg25C.
During 20-hydroxyecdysone-induced eversion of imaginal discs a glycoprotein termed gp125 becomes detectable.
Identification: Via a genomic clone (pDCg2) selected from a D.melanogaster lambda library using a cDNA probe for chicken proα2(I) collagen.
Structural gene for a type IV basement-membrane collagen. Expression first detected following germ-band shortening in cells of both somatic and visceral mesodermal origin, specifically in mesoblasts (hemocytes) and fat-body cells (Mirre, Cecchini, LeParco and Knibiehler, 1988). Translation takes place in embryonic mesoblasts and larval fat-body cells and is deposited extracellularly in basement membranes surrounding skeletal and visceral muscles (LeParco, Bevic, Knibiehler, Mirre and Cecchini, 1989).
Source for identity of: Col4a1 Cg25C
Renamed from 'Cg25C' to 'Col4a1' to provide more informative & accurate nomenclature and to reflect usage in key publications, including FBrf0129868, FBrf0217234, and FBrf0231085.
