FlyBase Gene Report: Dmel\mRpL4

General Information

Symbol

Dmel\mRpL4

Species

D. melanogaster

Name

mitochondrial ribosomal protein L4

Annotation Symbol

CG5818

Feature Type

protein_coding_gene

FlyBase ID

FBgn0001995

Gene Model Status

Current

Stock Availability

7 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Pathway
Gene Group
Interactive Fly

Also Known As

l(2)35Fe, BG:DS02740.17 , l(2)k14608

Key Links

Genomic Location

Cytogenetic map

35F1-35F1

Sequence location

2L:16,328,059..16,329,132 [-]

Recombination map

2-52

RefSeq locus

NT_033779 REGION:16328059..16329132

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables structural constituent of ribosome

inferred from biological aspect of ancestor with PANTHER:PTN000078778

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR002136, InterPro:IPR023574

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with UniProtKB:P51998

(Gene Ontology Curators, 2002-)

traceable author statement

(Marygold et al., 2007)

Biological Process (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in positive regulation of Notch signaling pathway

inferred from genetic interaction with FLYBASE:Su(H); FB:FBgn0004837

(Mo et al., 2023)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in mitochondrial translation

traceable author statement

(Marygold et al., 2007)

involved_in positive regulation of Notch signaling pathway

inferred from biological aspect of ancestor with PANTHER:PTN000844076

(GO Reference Genome Project, 2011-)

involved_in regulation of translation

inferred from biological aspect of ancestor with PANTHER:PTN000078778

(GO Reference Genome Project, 2011-)

involved_in translation

inferred from electronic annotation with InterPro:IPR002136, InterPro:IPR023574

(InterPro Project Members, 2004-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

is_active_in nucleus

inferred from direct assay

(Mo et al., 2023)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

part_of mitochondrial large ribosomal subunit

traceable author statement

(Marygold et al., 2007)

inferred from biological aspect of ancestor with PANTHER:PTN000078780

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity with UniProtKB:P51998

(Gene Ontology Curators, 2002-)

is_active_in ribosome

inferred from biological aspect of ancestor with PANTHER:PTN000078778

(GO Reference Genome Project, 2011-)

located_in ribosome

inferred from electronic annotation with InterPro:IPR002136, InterPro:IPR023574

(InterPro Project Members, 2004-)

Gene Group (FlyBase)

MITOCHONDRIAL LARGE RIBOSOMAL SUBUNIT

Pathway (FlyBase)

POSITIVE REGULATORS OF NOTCH SIGNALING PATHWAY

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

MITOCHONDRIAL LARGE RIBOSOMAL SUBUNIT -: Mitochondrial ribosomes are found in the mitochondrial matrix and translate genes involved in oxidative phosphorylation encoded by the mitochondrial genome. Ribosomes are formed by two ribonucleoprotein subunits, large and small. The principle function of the large subunit is to bind tRNA during the synthesis of polypeptides. (Adapted from FBrf0205398).

Pathway (FlyBase)

POSITIVE REGULATORS OF NOTCH SIGNALING PATHWAY -: The Notch receptor signaling pathway is activated by the binding of the transmembrane receptor Notch (N) to transmembrane ligands, Dl or Ser, presented on adjacent cells. This results in the proteolytic cleavage of N, releasing the intracellular domain (NICD). NICD translocates into the nucleus, interacting with Su(H) and mam to form a transcription complex, which up-regulates transcription of Notch-responsive genes. Positive regulators of the pathway enhance the signal from the sending cell or the response in the receiving cell. (Adapted from FBrf0225731 and FBrf0192604).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\mRpL4 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9V3D0)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.48

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

mRpL4-RA

FBtr0080843

NM_080200

1012

296

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

mRpL4-PA

FBpp0080401

32.8

296

9.63

Q9V3D0

NP_524939

AAF53526

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9V3D0

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mRpL4 using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.31

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

is_active_in nucleus

inferred from direct assay

(Mo et al., 2023)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\mRpL4 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\mRpL4

View exonic expression by tissue for Dmel\mRpL4

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

49425/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0001995

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001995

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0001995

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5818

Flygut - An atlas of the Drosophila adult midgut

FBgn0001995

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 4 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of mRpL4

Transgenic constructs containing regulatory region of mRpL4

Aberrations (Deficiencies and Duplications) ( 108 )

Inferred from experimentation ( 108 )

Gene disrupted in

Df(2L)cact-255rv64

(Dawson et al., 1995, Tower et al., 1993)

Df(2L)ED1123

(Marygold et al., 2007)

Df(2L)Exel7065

(Marygold et al., 2007, Ryder, 2004.4.26)

Df(2L)k08106-rv4

(Roote, 2000.6.13)

Df(2L)k08106-rv6

(Roote, 2000.6.13, Roote, 1999.7.7)

Df(2L)k08106-rv15

(Roote, 2000.6.13, Roote, 1999.7.7)

(Roote, 2000.6.13)

(Roote, 2000.6.13)

(Roote, 2000.6.13)

(Roote, 2000.6.13)

(Roote, 2000.6.13)

(Roote, 2000.6.13)

(Roote, 2000.6.13)

(Roote, 2000.6.13)

(Marygold et al., 2007, Ashburner et al., 1990)

Df(2L)RA5

(Levine et al., 2007, Marygold et al., 2007, Eberl et al., 1998, Roote, 1996.8, Ashburner et al., 1990)

Df(2L)RM5

(Ashburner et al., 1990)

Df(2L)RN2

(Eberl et al., 1998, Dawson et al., 1995, Ashburner et al., 1990)

Df(2L)TE35D-16

(Ashburner et al., 1990)

Df(2L)TE35D-21

(Roote, 1996.8, Dawson et al., 1995, Ashburner et al., 1990)

Df(2L)TE35D-23

(Ashburner et al., 1990)

Gene not disrupted in

Df(2L)64j

(Ashburner et al., 1990)

Df(2L)A47

(Fossett et al., 1994)

Df(2L)A48

(Fossett et al., 1994)

Df(2L)A63

(Fossett et al., 1994)

Df(2L)A72

(Fossett et al., 1994, Ashburner et al., 1990)

Df(2L)A178

(Fossett et al., 1994)

Df(2L)A215

(Fossett et al., 1994)

Df(2L)A217

(Fossett et al., 1994)

Df(2L)A220

(Fossett et al., 1994)

Df(2L)A245

(Fossett et al., 1994)

Df(2L)A246

(Fossett et al., 1994)

Df(2L)A264

(Fossett et al., 1994)

Df(2L)A266

(Fossett et al., 1994)

Df(2L)A267

(Fossett et al., 1994, Ashburner et al., 1990)

Df(2L)A337

(Fossett et al., 1994)

Df(2L)A376

(Fossett et al., 1994)

Df(2L)A379

(Fossett et al., 1994)

Df(2L)A400

(Fossett et al., 1994)

Df(2L)A445

(Fossett et al., 1994)

Df(2L)Adh-nBR100

(Fossett et al., 1994)

Df(2L)Adh-nBR101

(Fossett et al., 1994)

Df(2L)Adh-nBR102

(Fossett et al., 1994)

Df(2L)Adh-nBR103

(Fossett et al., 1994)

Df(2L)Adh-nBR105

(Fossett et al., 1994)

Df(2L)Adh-nBR117

(Fossett et al., 1994)

Df(2L)Adh-nBR118

(Fossett et al., 1994)

Df(2L)Adh-nBR119

(Fossett et al., 1994)

Df(2L)Adh-nBR120

(Fossett et al., 1994)

Df(2L)Adh-nBR121

(Fossett et al., 1994)

Df(2L)Adh-nBR122

(Fossett et al., 1994)

Df(2L)Adh-nBR125

(Fossett et al., 1994)

Df(2L)Adh-nBR126

(Fossett et al., 1994)

Df(2L)Adh-nBR127

(Fossett et al., 1994)

Df(2L)Adh-nBR128

(Fossett et al., 1994)

Df(2L)Adh-nBR129

(Fossett et al., 1994)

Df(2L)Adh-nBR130

(Fossett et al., 1994)

Df(2L)Adh-nBR131

(Fossett et al., 1994)

Df(2L)ARR1

(Ashburner et al., 1990)

Df(2L)b80e4

(Ashburner et al., 1990)

Df(2L)el20

(Ashburner et al., 1990)

Df(2L)el28

(Ashburner et al., 1990)

Df(2L)fn12

(Ashburner et al., 1990)

Df(2L)fn26

(Ashburner et al., 1990)

Df(2L)fn27

(Ashburner et al., 1990)

Df(2L)H20

(Ashburner et al., 1990)

Df(2L)H60-3

(Roote, 1996.1.11, Roote, 1996.8, Dawson et al., 1995)

(Roote, 1996.8)

(Roote, 1996.8)

(Roote, 2000.6.13)

(Roote, 2000.6.13)

(Levine et al., 2007)

Df(2L)n78l3

(Ashburner et al., 1990)

Df(2L)noc11

(Ashburner et al., 1990)

Df(2L)osp29

(Eberl et al., 1998)

Df(2L)osp38

(Ashburner et al., 1990)

Df(2L)rd9

(Ashburner et al., 1990)

Df(2L)Sco7

(Ashburner et al., 1990)

Df(2L)Sco^rv16

(Ashburner et al., 1990)

Df(2L)Sco^rv18

(Ashburner et al., 1990)

Df(2L)Sco^rv19

(Ashburner et al., 1990)

Df(2L)Sco^rv25

(Ashburner et al., 1990)

Df(2L)SS1

(Ashburner et al., 1990)

Df(2L)TE35BC-3

(Ashburner et al., 1990)

Df(2L)TE35BC-4

(Ashburner et al., 1990)

Df(2L)TE35BC-34

(Ashburner et al., 1990)

Df(2L)TE35D-1

(Ashburner et al., 1990)

Df(2L)TE35D-2

(Ashburner et al., 1990)

Df(2L)TE35D-3

(Ashburner et al., 1990)

Df(2L)TE35D-4

(Ashburner et al., 1990)

Df(2L)TE35D-6

(Ashburner et al., 1990)

Df(2L)TE35D-13

(Eberl et al., 1998, Dawson et al., 1995, Ashburner et al., 1990)

Df(2L)TE35D-14

(Eberl et al., 1998, Ashburner et al., 1990)

Df(2L)TE35D-15

(Ashburner et al., 1990)

Df(2L)TE35D-19

(Ashburner et al., 1990)

Df(2L)TE35D-20

(Ashburner et al., 1990)

Df(2L)TE35D-22

(Ashburner et al., 1990)

In(2L)dpp^s22Ldpp^d36R

(Ashburner et al., 1990)

In(2L)el18

(Eberl et al., 1998, Dawson et al., 1995, Ashburner et al., 1990)

In(2LR)Sco^rv1-DV7

(Ashburner et al., 1990)

T(2;3)Sco^rv7

(Ashburner et al., 1990)

Tp(2;2)A446

(Fossett et al., 1994)

Gene duplicated in

Dp(2;3)osp³

(Ashburner et al., 1990)

Gene not duplicated in

Dp(2;1)Sco^rv23

(Ashburner et al., 1990)

Dp(2;2)GYL

(Ashburner et al., 1990)

Dp(2;2)TE35B-54^L

(Ashburner et al., 1990)

In(2L)C163.41^LC158^R

(Ashburner et al., 1990)

In(2L)Sco^rv17LC158^R

(Ashburner et al., 1990)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | recessive

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

mRpL4^GD11777

viable, with Scer\GAL4^Mef2.PR

mRpL4^GD11777

viable, with Scer\GAL4^pnr-MD237

mRpL4^GD11777

viable, with Scer\GAL4^tin.CΔ4

mRpL4^GD11777

Other Phenotypes

Allele

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

mRpL4^GD11777

decreased occurrence of cell division | larval stage

mRpL4^k14608

decreased occurrence of cell division | somatic clone | third instar larval stage

mRpL4^k14608

visible | recessive | somatic clone

mRpL4^k14608

Phenotype manifest in

Allele

eye | somatic clone

mRpL4^k14608

eye disc | somatic clone

mRpL4^k14608

lens | somatic clone

mRpL4^k14608

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (3)

Hsap\MRPL4

14 of 14

Yes

Hsap\RPL4

1 of 14

Hsap\RPS16

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (3)

Rnor\Mrpl4

14 of 14

Yes

Rnor\Rpl4

1 of 14

Rnor\Rps16

1 of 14

Mus musculus (laboratory mouse) (3)

Mmus\Mrpl4

13 of 14

Yes

Mmus\Rpl4

1 of 14

Mmus\Rps16

1 of 14

Xenopus tropicalis (Western clawed frog) (1)

Xtro\mrpl4

8 of 13

Yes

Danio rerio (Zebrafish) (3)

Drer\mrpl4

14 of 14

Yes

Drer\rpl4

1 of 14

Drer\rps16

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (3)

Cele\mrpl-4

14 of 14

Yes

Cele\rpl-4

1 of 14

Cele\rps-16

1 of 14

Anopheles gambiae (African malaria mosquito) (3)

Agam\AgaP_AGAP007937

12 of 12

Yes

Agam\AgaP_AGAP002306

1 of 12

Agam\AgaP_AGAP011424

1 of 12

Arabidopsis thaliana (thale-cress) (7)

Atha\AT2G20060

12 of 13

Yes

Atha\RPL4

5 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (5)

Scer\YML6

7 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (5)

Spom\SPBC2D10.08c

8 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (1)

Ecol\rplD

10 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:mRpL4. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (2)

RpL4

1 of 13

RpS16

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

cancer, intercellular interactions, RAS-mito

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

MRPL4; mitochondrial ribosomal protein L4

14 of 14

611823

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

mRpL4 - mRpL50

two hybrid

(Mo et al., 2023)

mRpL4 - mnb

anti tag coimmunoprecipitation, western blot

(Mo et al., 2023)

mRpL4 - wap

anti tag coimmunoprecipitation, western blot, two hybrid

(Mo et al., 2023)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

mRpL4

(Krupp et al., 2005)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0001995

MIST (protein-protein) - An integrated Molecular Interaction Database

49425

Pathways

Signaling Pathways (FlyBase)

POSITIVE REGULATORS OF NOTCH SIGNALING PATHWAY

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Ribosome

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-52

Cytogenetic map

35F1-35F1

Sequence location

2L:16,328,059..16,329,132 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

35F1-35F1

Limits computationally determined from genome sequence between P{EP}fzy^EP1028&P{lacW}mRpL4^k14608 and P{PZ}dac^P

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

35F11-35F12

(determined by in situ hybridisation)

(Ashburner et al., 1999, Spradling et al., 1999)

35F11-35F12

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

2-52

(FlyBase, 2016)

2-49.7

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (7)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

12179

y¹ w^67c23; P{lacW}mRpL4^k14608/CyO

3620

Adc^b-1 mRpL4¹ pr¹ cn¹ wx^wxt bw¹/CyO

Kyoto

111435

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{lacW}mRpL4^k14608 P{neoFRT}40A/CyO y⁺

NIG-Fly

5818R-1

P{NIG.5818R}1

2LG-0302

y² VhaAC39-1^cho-1 v¹; P{U6-mRpL4.NIG.sgRNA}attP40/CyO

VDRC

v101351

P{KK108552}VIE-260B

v40608

w¹¹¹⁸; P{GD11777}v40608/CyO

Genomic Clones (20)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (79)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD33485

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0001995

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0001995

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Mo et al., 2023)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Area matching Drosophila EST AA979191.

(Davies, 2001.3.15)

Identification: One of a collection of genes identified with defective larval growth that extend larval life. Not studied further due to apparent lack of association of mutant phenotype with insertion of P-element present in the mutant chromosome.

(Galloni and Edgar, 1999)

2 alleles are discussed but are not named.

(Ashburner et al., 1990)

Relationship to Other Genes

Source for database merge of

Source for merge of: l(2)35Fe CG5818

(Misra, 2000.4.12)

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

BG:DS02740.17

(Ashburner et al., 1999, Ashburner et al., 1999.10.12)

CG5818

(National Institute of Genetics Fly Stocks, 2022-, Choo et al., 2015, Fukuoh et al., 2014, Keleman et al., 2009.8.5, Marygold et al., 2007)

(Suzuki et al., 2001)

Rpl4

(Ashburner et al., 1999.10.12)

l(2)35Fe

(Levine et al., 2007, Bolshakov et al., 2002, Roote, 2000.6.13, Ashburner et al., 1999, Ashburner et al., 1999, Ashburner et al., 1999, Landis and Tower, 1999, Spradling et al., 1999, Tower, 1999.12.23, Chen et al., 1998, Eberl et al., 1998, Roote, 1996.1.11, Spradling et al., 1995, BDGP Project Members, 1994-1999, Ashburner et al., 1990)

l(2)k14608

(Galloni and Edgar, 1999)

l35Fe

(Fossett et al., 1994)

mRpL4

(Mo et al., 2023, National Institute of Genetics Fly Stocks, 2022-, Japanese National Institute of Genetics, 2012.5.21, Ohsawa et al., 2012, Marygold et al., 2007, Liao et al., 2006, Mandal et al., 2005)

mito

(Enomoto and Igaki, 2022)

mrpL4

(Wang et al., 2012)

Name Synonyms

lethal(2)35Fe

mitochondrial ribosomal protein L4

(Suzuki et al., 2001)

mitochondrial ribosomal protein large subunit 4

(Liao et al., 2006)

Secondary FlyBase IDs

FBgn0032584

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 36 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

49425

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9V3D0

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q9V3D0

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9V3D0

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

49425/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0001995

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0001995

FlyMine - An integrated database for Drosophila genomics

FBgn0001995

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG5818

MARRVEL_MODEL - MARRVEL (model organism gene)

49425

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0001995

DroID - A comprehensive database of gene and protein interactions.

FBgn0001995

DRSC - Results frm RNAi screens

FBgn0001995

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001995

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0001995

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5818

Flygut - An atlas of the Drosophila adult midgut

FBgn0001995

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0001995

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC011518

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Ribosome

MIST (protein-protein) - An integrated Molecular Interaction Database

49425

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (67)

The following external sites may use different assemblies or annotations than FlyBase.

1.31

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