FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\tsu
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General Information
Symbol
Dmel\tsu
Species
D. melanogaster
Name
tsunagi
Annotation Symbol
CG8781
Feature Type
protein_coding_gene
FlyBase ID
FBgn0033378
Gene Model Status
Current
Stock Availability
10 publicly available
Gene Summary
Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs (PubMed:14973490, PubMed:24967911). Involved in exon definition of genes containing long introns, including the rolled/MAPK gene (PubMed:20946982, PubMed:20946983). The mago-tsu heterodimer interacts with the EJC key regulator Pym leading to EJC disassembly in the cytoplasm (PubMed:24967911). Has a role in oskar mRNA localization to the posterior pole of the developing oocyte (PubMed:11691839). (UniProt, Q9V535)
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

Y14, Y14/Tsunagi

Key Links
Genomic Location
Cytogenetic map
45A7-45A7
Sequence location
Recombination map
2-60
RefSeq locus
NT_033778 REGION:9110799..9111578
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (19 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables protein binding
inferred from physical interaction with UniProtKB:P82804
(Bono et al., 2004, Forler et al., 2003)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
enables mRNA binding
inferred from biological aspect of ancestor with PANTHER:PTN000566947
(GO Reference Genome Project, 2011-)
inferred from electronic annotation with InterPro:IPR033744
(InterPro Project Members, 2004-)
inferred from sequence or structural similarity with UniProtKB:Q9Y5S9
(Mohr et al., 2001)
inferred from sequence or structural similarity
(Lasko, 2000)
enables nucleic acid binding
inferred from electronic annotation with InterPro:IPR035979
(InterPro Project Members, 2004-)
enables RNA binding
inferred from electronic annotation with InterPro:IPR000504, InterPro:IPR008111
(InterPro Project Members, 2004-)
Biological Process (11 terms)
Terms Based on Experimental Evidence (7 terms)
CV Term
Evidence
References
involved_in germarium-derived oocyte fate determination
inferred from mutant phenotype
(Parma et al., 2007)
involved_in microtubule cytoskeleton organization
inferred from mutant phenotype
(Mohr et al., 2001)
involved_in oocyte anterior/posterior axis specification
inferred from mutant phenotype
(Mohr et al., 2001)
involved_in oocyte dorsal/ventral axis specification
inferred from mutant phenotype
(Mohr et al., 2001)
involved_in oocyte nucleus localization involved in oocyte dorsal/ventral axis specification
inferred from mutant phenotype
(Mohr et al., 2001)
involved_in regulation of pole plasm oskar mRNA localization
inferred from mutant phenotype
(Mohr et al., 2001)
inferred from physical interaction with FLYBASE:mago; FB:FBgn0002736
(Mohr et al., 2001)
involved_in RNA splicing
inferred from mutant phenotype
(Hayashi et al., 2014)
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
involved_in establishment of pole plasm mRNA localization
traceable author statement
(Farina and Singer, 2002)
involved_in mRNA splicing, via spliceosome
inferred by curator from GO:0071013
(Herold et al., 2009)
involved_in pole plasm oskar mRNA localization
traceable author statement
(Farina and Singer, 2002, Tekotte and Davis, 2002)
involved_in RNA processing
inferred from electronic annotation with InterPro:IPR008111
(InterPro Project Members, 2004-)
involved_in RNA splicing
inferred from biological aspect of ancestor with PANTHER:PTN000566947
(GO Reference Genome Project, 2011-)
Cellular Component (4 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
part_of catalytic step 2 spliceosome
inferred from high throughput direct assay
(Herold et al., 2009)
located_in cytoplasm
inferred from direct assay
(Parma et al., 2007, Mohr et al., 2001)
part_of exon-exon junction complex
inferred from physical interaction with FLYBASE:mago; FB:FBgn0002736
(Lewandowski et al., 2010)
located_in nucleus
inferred from direct assay
(Parma et al., 2007, Le Hir et al., 2001, Mohr et al., 2001)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
located_in cytoplasm
inferred from electronic annotation with InterPro:IPR008111
(InterPro Project Members, 2004-)
part_of exon-exon junction complex
inferred from biological aspect of ancestor with PANTHER:PTN000566947
(GO Reference Genome Project, 2011-)
located_in nucleus
inferred from electronic annotation with InterPro:IPR008111
(InterPro Project Members, 2004-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the RBM8A family. (Q9V535)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
SPLICEOSOME COMPLEX C -
Nuclear pre-mRNA splicing is catalyzed by the spliceosome which assembles in a step-wise manner. Complex B undergoes a number of structural rearrangements and U4 and U1 snRNPs dissociate to generate complex C, the catalytic spliceosome. (Adapted from PMID:24452469 and PMID:23118483).
SPLICEOSOME COMPLEX P -
Nuclear pre-mRNA splicing is catalyzed by the spliceosome which assembles in a step-wise manner. Complex P, the post-spliceosomal complex, is formed after the catalytic removal of the intron by complex C. Complex P contains the lariat intron and spliced exons. (Adapted from PMID:24452469 and PMID:23118483).
Protein Function (UniProtKB)
Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs (PubMed:14973490, PubMed:24967911). Involved in exon definition of genes containing long introns, including the rolled/MAPK gene (PubMed:20946982, PubMed:20946983). The mago-tsu heterodimer interacts with the EJC key regulator Pym leading to EJC disassembly in the cytoplasm (PubMed:24967911). Has a role in oskar mRNA localization to the posterior pole of the developing oocyte (PubMed:11691839).
(UniProt, Q9V535)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\tsu for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9V535)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
PDB - An information portal to biological macromolecular structures
Comments on Gene Model

Gene model reviewed during 5.44

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.49

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
tsu-RA
FBtr0088567
NM_136610
691
165
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
tsu-PA
FBpp0087648
19.0
165
4.92
Q9V535
NP_610454
AAF58987
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Heterodimer (via RRM domain) with mago (PubMed:11691839, PubMed:12704080, PubMed:12730685). Part of the mRNA splicing-dependent exon junction complex (EJC) complex; the core complex contains btz/CASC3, eIF4AIII, mago and tsu/RBM8A (PubMed:14973490). Interacts with Pym (via N-terminus); the interaction is direct (PubMed:14968132, PubMed:24967911). Interacts with eIF4AIII (PubMed:14973490).

(UniProt, Q9V535)
Domain

The RNA recognition motif (RRM) is involved in the interaction with mago. The RNA-binding activity of such domains is therefore uncertain.

(UniProt, Q9V535)
Crossreferences
InterPro - A database of protein families, domains and functional sites
PDB - An information portal to biological macromolecular structures
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\tsu using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.05

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism
(Fisher et al., 2012)
embryonic stage 7 -- 8
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 9 -- 10
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 11 -- 12
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic brain
(Fisher et al., 2012)
larval ventral nerve cord
(Fisher et al., 2012)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference

Comment: expression assayed in cultured cells

(Gatfield and Izaurralde, 2002)
Additional Descriptive Data

tsu transcripts are expressed in S2 cells.

(Gatfield and Izaurralde, 2002)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
oogenesis & adult stage | female
oocyte
(Parma et al., 2007)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
part_of catalytic step 2 spliceosome
inferred from high throughput direct assay
(Herold et al., 2009)
located_in cytoplasm
inferred from direct assay
(Parma et al., 2007, Mohr et al., 2001)
part_of exon-exon junction complex
inferred from physical interaction with FLYBASE:mago; FB:FBgn0002736
(Lewandowski et al., 2010)
located_in nucleus
inferred from direct assay
(Parma et al., 2007, Le Hir et al., 2001, Mohr et al., 2001)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\tsu in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 10 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 12 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of tsu
Transgenic constructs containing regulatory region of tsu
Aberrations (Deficiencies and Duplications) ( 9 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal, with Scer\GAL4insc-Mz1407
lethal, with Scer\GAL4nub-AC-62
lethal (with Df(2R)BSC271)
lethal (with Df(2R)Np5)
lethal | female limited, with Scer\GAL4dome-PG14
lethal | recessive
lethal - all die before end of pupal stage, with Scer\GAL4pnr-MD237
lethal - all die during embryonic stage | germline clone | recessive
lethal - all die during P-stage, with Scer\GAL4Mef2.PR
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4tin.CΔ4
viable (with tsu5)
viable (with tsu6)
viable (with tsu7)
Sterility
Allele
female sterile, with Scer\GAL4VP16.otu
female sterile (with tsu5)
female sterile (with tsu6)
female sterile (with tsu7)
female sterile | recessive
Other Phenotypes
Allele
abnormal cell migration | oogenesis, with Scer\GAL4c306
abnormal cell polarity | oogenesis, with Scer\GAL4c306
short lived | heat sensitive, with Scer\GAL4tin.CΔ4
some die during embryonic stage | recessive
some die during pupal stage, with Scer\GAL4pnr-MD237
Phenotype manifest in
Allele
border follicle cell, with Scer\GAL4c306
dorsal appendage
dorsal appendage (with tsu6)
dorsal appendage (with tsu7)
egg
egg (with tsu6)
egg (with tsu7)
egg | germline clone
egg chamber (with tsu6)
egg chamber (with tsu7)
egg chamber | germline clone
germarium region 2b | germline clone
germarium region 3 | germline clone
larval brain | larval stage, with Scer\GAL4insc-Mz1407
oocyte nucleus
ovary, with Scer\GAL4nanos.PG
ovary, with Scer\GAL4NP1624
synaptonemal complex | germline clone
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (48)
Hsap\RBM8A
13 of 14
Yes
Yes
Hsap\CIRBP
1 of 14
No
No
Hsap\CSTF2
1 of 14
No
No
Hsap\CSTF2T
1 of 14
No
No
Hsap\EIF3G
1 of 14
No
No
1  
Hsap\ELAVL1
1 of 14
No
No
3  
Hsap\ELAVL2
1 of 14
No
No
2  
Hsap\ELAVL3
1 of 14
No
No
3  
Hsap\HNRNPA3
1 of 14
No
No
2  
Hsap\HNRNPAB
1 of 14
No
No
Hsap\HNRNPDL
1 of 14
No
No
4  
Hsap\NCBP2
1 of 14
No
No
Hsap\NCL
1 of 14
No
No
Hsap\NIFK
1 of 14
No
No
Hsap\PABPC4L
1 of 14
No
No
Hsap\PPIE
1 of 14
No
No
Hsap\PUF60
1 of 14
No
No
Hsap\RBFOX3
1 of 14
No
No
Hsap\RBM7
1 of 14
No
No
Hsap\RBM18
1 of 14
No
No
1  
Hsap\RBM19
1 of 14
No
No
1  
Hsap\RBM23
1 of 14
No
No
Hsap\RBM28
1 of 14
No
No
1  
Hsap\RBM34
1 of 14
No
No
Hsap\RBM39
1 of 14
No
No
Hsap\RBMX2
1 of 14
No
No
1  
Hsap\RBMX
1 of 14
No
No
Hsap\RBMXL1
1 of 14
No
No
Hsap\RBMXL3
1 of 14
No
Yes
Hsap\RBMY1A1
1 of 14
No
No
Hsap\RBMY1B
1 of 14
No
No
Hsap\RBMY1C
1 of 14
No
Yes
Hsap\RBMY1D
1 of 14
No
No
Hsap\RBMY1E
1 of 14
No
Yes
Hsap\RBMY1F
1 of 14
No
Yes
Hsap\RBMY1J
1 of 14
No
No
Hsap\SART3
1 of 14
No
No
Hsap\SF3B4
1 of 14
No
No
Hsap\SNRNP35
1 of 14
No
No
Hsap\SNRNP70
1 of 14
No
No
Hsap\SRSF8
1 of 14
No
No
Hsap\SRSF12
1 of 14
No
No
Hsap\TIA1
1 of 14
No
No
Hsap\TRA2A
1 of 14
No
No
2  
Hsap\TRA2B
1 of 14
No
No
1  
Hsap\U2AF2
1 of 14
No
No
4  
Hsap\U2SURP
1 of 14
No
No
Hsap\ZCRB1
1 of 14
No
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (26)
Rnor\Rbm8a
13 of 14
Yes
Yes
Rnor\Cstf2
1 of 14
No
No
Rnor\Cstf2t
1 of 14
No
No
Rnor\Elavl3
1 of 14
No
No
Rnor\Hnrnpa3
1 of 14
No
No
Rnor\Hnrnpab
1 of 14
No
No
Rnor\Hnrnpdl
1 of 14
No
No
Rnor\Myef2
1 of 14
No
No
Rnor\Ncbp2
1 of 14
No
No
Rnor\Ncl
1 of 14
No
No
Rnor\Nifk
1 of 14
No
No
Rnor\Pabpc1l
1 of 14
No
No
Rnor\Pabpc4l
1 of 14
No
No
Rnor\Ppie
1 of 14
No
No
Rnor\Puf60
1 of 14
No
No
Rnor\Rbm19
1 of 14
No
No
Rnor\Rbm34
1 of 14
No
No
Rnor\Rbm39
1 of 14
No
No
Rnor\Rbmx2
1 of 14
No
No
Rnor\Sf3b4
1 of 14
No
No
Rnor\Snrnp35
1 of 14
No
No
Rnor\Snrnp70
1 of 14
No
No
Rnor\Srsf8
1 of 14
No
No
Rnor\Tra2a
1 of 14
No
No
Rnor\Tra2b
1 of 14
No
No
Rnor\U2af2
1 of 14
No
No
Mus musculus (laboratory mouse) (24)
Mmus\Rbm8a
13 of 14
Yes
Yes
Mmus\Rbm8a2
9 of 14
No
Yes
Mmus\4930595M18Rik
1 of 14
No
No
Mmus\Cstf2
1 of 14
No
No
Mmus\Cstf2t
1 of 14
No
No
Mmus\Elavl2
1 of 14
No
No
Mmus\Elavl3
1 of 14
No
No
Mmus\Gm11639
1 of 14
No
No
Mmus\Hnrnpa3
1 of 14
No
No
Mmus\Ncbp2
1 of 14
No
No
Mmus\Ncl
1 of 14
No
No
Mmus\Nifk
1 of 14
No
No
Mmus\Pabpc1l
1 of 14
No
No
Mmus\Pabpc4l
1 of 14
No
No
Mmus\Ppie
1 of 14
No
No
Mmus\Puf60
1 of 14
No
No
Mmus\Rbm34
1 of 14
No
No
Mmus\Rbmx2
1 of 14
No
No
Mmus\Sf3b4
1 of 14
No
No
Mmus\Snrnp35
1 of 14
No
No
Mmus\Snrnp70
1 of 14
No
No
Mmus\Tra2a
1 of 14
No
No
Mmus\Tra2b
1 of 14
No
No
Mmus\U2af2
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (20)
Xtro\rbm8a
9 of 13
Yes
Yes
Xtro\celf1
1 of 13
No
No
Xtro\cirbp
1 of 13
No
No
Xtro\eif3g
1 of 13
No
No
Xtro\elavl1
1 of 13
No
No
Xtro\hnrnpdl
1 of 13
No
No
Xtro\jpt2
1 of 13
No
No
Xtro\ncbp2
1 of 13
No
No
Xtro\nifk
1 of 13
No
No
Xtro\rbm34
1 of 13
No
No
Xtro\rnps1
1 of 13
No
No
Xtro\sf3b4
1 of 13
No
No
Xtro\snrnp70
1 of 13
No
No
Xtro\srsf4
1 of 13
No
No
Xtro\tia1
1 of 13
No
No
Xtro\tra2a
1 of 13
No
No
Xtro\tra2b
1 of 13
No
No
Xtro\tut1
1 of 13
No
No
Xtro\u2surp
1 of 13
No
No
Xtro\zcrb1
1 of 13
No
Yes
Danio rerio (Zebrafish) (32)
Drer\rbm8a
14 of 14
Yes
Yes
Drer\celf2
1 of 14
No
No
Drer\cirbpa
1 of 14
No
No
Drer\cstf2
1 of 14
No
No
Drer\elavl3
1 of 14
No
No
Drer\hnrnpm
1 of 14
No
No
Drer\LOC100536928
1 of 14
No
Yes
Drer\LOC100537585
1 of 14
No
Yes
Drer\LOC101885013
1 of 14
No
Yes
Drer\LOC110437789
1 of 14
No
Yes
Drer\ncbp2
1 of 14
No
No
Drer\nifk
1 of 14
No
No
Drer\pabpc1a
1 of 14
No
No
Drer\ppie
1 of 14
No
No
Drer\puf60a
1 of 14
No
No
Drer\puf60b
1 of 14
No
No
Drer\rbm7
1 of 14
No
No
Drer\rbm11
1 of 14
No
No
Drer\rbmx2
1 of 14
No
No
Drer\rbmx
1 of 14
No
Yes
Drer\sf3b4
1 of 14
No
No
Drer\si:ch73-190f9.4
1 of 14
No
Yes
Drer\snrnp35
1 of 14
No
Yes
Drer\snrnp70
1 of 14
No
No
Drer\srsf7a
1 of 14
No
No
Drer\syncrip
1 of 14
No
No
Drer\tia1l
1 of 14
No
No
Drer\tra2a
1 of 14
No
No
Drer\tra2b
1 of 14
No
No
Drer\u2af2a
1 of 14
No
No
Drer\u2af2b
1 of 14
No
No
Drer\zgc:171310
1 of 14
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (21)
Cele\rnp-4
14 of 14
Yes
Yes
Cele\asd-1
1 of 14
No
No
Cele\cpf-2
1 of 14
No
No
Cele\cyn-13
1 of 14
No
No
Cele\eif-3.G
1 of 14
No
No
Cele\exc-7
1 of 14
No
No
Cele\nifk-1
1 of 14
No
No
Cele\R06C1.4
1 of 14
No
No
Cele\rbd-1
1 of 14
No
No
Cele\rbm-3.1
1 of 14
No
No
Cele\rbm-3.2
1 of 14
No
No
Cele\rbm-7
1 of 14
No
No
Cele\rbm-34
1 of 14
No
No
Cele\rbmx-2
1 of 14
No
No
Cele\rnp-6
1 of 14
No
No
Cele\rnp-7
1 of 14
No
No
Cele\rnp-9
1 of 14
No
No
Cele\rsp-5
1 of 14
No
No
Cele\rsp-8
1 of 14
No
No
Cele\sap-49
1 of 14
No
No
Cele\sig-7
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (32)
Agam\AgaP_AGAP006365
12 of 12
Yes
Yes
Agam\AgaP_AGAP000965
1 of 12
No
No
Agam\AgaP_AGAP000977
1 of 12
No
No
Agam\AgaP_AGAP001460
1 of 12
No
No
Agam\AgaP_AGAP001538
1 of 12
No
No
Agam\AgaP_AGAP001883
1 of 12
No
No
Agam\AgaP_AGAP001930
1 of 12
No
No
Agam\AgaP_AGAP002256
1 of 12
No
No
Agam\AgaP_AGAP002335
1 of 12
No
No
Agam\AgaP_AGAP002908
1 of 12
No
No
Agam\AgaP_AGAP003219
1 of 12
No
No
Agam\AgaP_AGAP003899
1 of 12
No
No
Agam\AgaP_AGAP004414
1 of 12
No
No
Agam\AgaP_AGAP005292
1 of 12
No
No
Agam\AgaP_AGAP006090
1 of 12
No
No
Agam\AgaP_AGAP006733
1 of 12
No
No
Agam\AgaP_AGAP006755
1 of 12
No
No
Agam\AgaP_AGAP006798
1 of 12
No
No
Agam\AgaP_AGAP008136
1 of 12
No
No
Agam\AgaP_AGAP008433
1 of 12
No
No
Agam\AgaP_AGAP009142
1 of 12
No
No
Agam\AgaP_AGAP009570
1 of 12
No
No
Agam\AgaP_AGAP009810
1 of 12
No
No
Agam\AgaP_AGAP010553
1 of 12
No
No
Agam\AgaP_AGAP010918
1 of 12
No
No
Agam\AgaP_AGAP012137
1 of 12
No
No
Agam\AgaP_AGAP012250
1 of 12
No
No
Agam\AgaP_AGAP012853
1 of 12
No
No
Agam\AgaP_AGAP013051
1 of 12
No
No
Agam\AgaP_AGAP013420
1 of 12
No
Yes
Agam\EIF3G_ANOGA
1 of 12
No
No
Agam\NCBP2_ANOGA
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (72)
Atha\Y14
11 of 13
Yes
Yes
Atha\AT1G03457
1 of 13
No
No
Atha\AT1G60000
1 of 13
No
No
Atha\AT1G60900
1 of 13
No
No
Atha\AT1G73530
1 of 13
No
Yes
Atha\AT2G27330
1 of 13
No
Yes
Atha\AT2G43370
1 of 13
No
No
Atha\AT3G04500
1 of 13
No
No
Atha\AT3G08000
1 of 13
No
No
Atha\AT3G14100
1 of 13
No
No
Atha\AT3G46020
1 of 13
No
Yes
Atha\AT3G47120
1 of 13
No
No
Atha\AT4G19610
1 of 13
No
No
Atha\AT4G20030
1 of 13
No
Yes
Atha\AT4G26650
1 of 13
No
No
Atha\AT4G35785
1 of 13
No
No
Atha\AT5G03580
1 of 13
No
No
Atha\AT5G04600
1 of 13
No
No
Atha\AT5G04810
1 of 13
No
No
Atha\AT5G06210
1 of 13
No
Yes
Atha\AT5G08695
1 of 13
No
No
Atha\AT5G19960
1 of 13
No
Yes
Atha\AT5G51300
1 of 13
No
No
Atha\AT5G52545
1 of 13
No
No
Atha\AT5G54580
1 of 13
No
Yes
Atha\AT5G55550
1 of 13
No
No
Atha\AT5G55670
1 of 13
No
No
Atha\AT5G59860
1 of 13
No
Yes
Atha\ATRBP45C
1 of 13
No
No
Atha\ATRZ-1A
1 of 13
No
No
Atha\AtRZ-1b
1 of 13
No
No
Atha\ATU2AF65A
1 of 13
No
No
Atha\CBP20
1 of 13
No
No
Atha\CCR1
1 of 13
No
No
Atha\CID8
1 of 13
No
No
Atha\CID10
1 of 13
No
No
Atha\CID11
1 of 13
No
No
Atha\CID12
1 of 13
No
Yes
Atha\CID13
1 of 13
No
No
Atha\CP31B
1 of 13
No
No
Atha\CSTF64
1 of 13
No
No
Atha\CYP59
1 of 13
No
No
Atha\EIF3G1
1 of 13
No
No
Atha\FPA
1 of 13
No
No
Atha\GRP7
1 of 13
No
No
Atha\GR-RBP1
1 of 13
No
No
Atha\GR-RBP2
1 of 13
No
No
Atha\GR-RBP3
1 of 13
No
Yes
Atha\GR-RBP5
1 of 13
No
Yes
Atha\PAB1
1 of 13
No
No
Atha\PAB3
1 of 13
No
No
Atha\PAB5
1 of 13
No
No
Atha\PAB6
1 of 13
No
No
Atha\PAB7
1 of 13
No
No
Atha\RBP31
1 of 13
No
No
Atha\RBP45B
1 of 13
No
No
Atha\RBP47C
1 of 13
No
No
Atha\RPS19
1 of 13
No
No
Atha\RS2Z32
1 of 13
No
No
Atha\RS2Z33
1 of 13
No
No
Atha\RS31a
1 of 13
No
No
Atha\RS31
1 of 13
No
No
Atha\RS40
1 of 13
No
No
Atha\RS41
1 of 13
No
No
Atha\SC35
1 of 13
No
No
Atha\SCL30A
1 of 13
No
No
Atha\SR34b
1 of 13
No
No
Atha\SR45a
1 of 13
No
No
Atha\SUA
1 of 13
No
No
Atha\U1-70K
1 of 13
No
No
Atha\UBP1A
1 of 13
No
No
Atha\UBP1B
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (10)
Scer\CBC2
1 of 13
Yes
No
Scer\HRP1
1 of 13
Yes
No
Scer\IST3
1 of 13
Yes
No
Scer\MRD1
1 of 13
Yes
No
Scer\NOP15
1 of 13
Yes
No
Scer\NSR1
1 of 13
Yes
Yes
Scer\PAB1
1 of 13
Yes
No
Scer\RNA15
1 of 13
Yes
No
Scer\SGN1
1 of 13
Yes
No
Scer\SNP1
1 of 13
Yes
No
Schizosaccharomyces pombe (Fission yeast) (10)
Spom\SPAC23A1.09
9 of 12
Yes
Yes
Spom\crp79
1 of 12
No
Yes
Spom\ctf1
1 of 12
No
No
Spom\cwf29
1 of 12
No
No
Spom\pabp
1 of 12
No
No
Spom\SPAC328.05
1 of 12
No
No
Spom\SPAC644.16
1 of 12
No
No
Spom\SPBC4F6.14
1 of 12
No
No
Spom\SPCC1827.05c
1 of 12
No
No
Spom\usp101
1 of 12
No
No
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:tsu. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (21)
Caper
1 of 13
Cbp20
1 of 13
CG4806
1 of 13
CG6937
1 of 13
CG10466
1 of 13
CG14414
1 of 13
CG34334
1 of 13
CG34354
1 of 13
CstF64
1 of 13
cyp33
1 of 13
fne
1 of 13
hfp
1 of 13
Ppil4
1 of 13
Rbp9
1 of 13
Rnp4F
1 of 13
Rox8
1 of 13
snRNP-U1-70K
1 of 13
Spx
1 of 13
sqd
1 of 13
tra2
1 of 13
trv
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Human Ortholog
    Disease
    Evidence
    References
    RBM8A; RNA binding motif protein 8A
    model of  thrombocytopenia-absent radius syndrome
    IEA
    (FlyBase, 2019-)
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    RBM8A; RNA binding motif protein 8A
    13 of 14
    605313
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    Interaction Browsers
    View in FlyBase Interactions Browser   View in MIST tool (external link)
    Please see the Physical Interaction reports below for full details
    RNA-protein
    Physical Interaction
    Assay
    References
    tsu - Motor
    anti tag coimmunoprecipitation, quantitative reverse transcription pcr
    (Saulière et al., 2010)
    tsu - ftz
    anti tag coimmunoprecipitation, autoradiography
    (Ghosh et al., 2012)
    tsu - msl-3
    pull down, western blot
    (Buscaino et al., 2003)
    tsu - osk
    anti tag coimmunoprecipitation, autoradiography
    (Ghosh et al., 2012)
    tsu - sp3
    anti tag coimmunoprecipitation, quantitative reverse transcription pcr
    (Saulière et al., 2010)
    tsu - su(wa)
    anti tag coimmunoprecipitation, quantitative reverse transcription pcr
    (Saulière et al., 2010)
    protein-protein
    Physical Interaction
    Assay
    References
    tsu - CG7483
    anti tag coimmunoprecipitation, western blot, pull down, molecular weight estimation by staining, anti bait coimmunoprecipitation
    (Obrdlik et al., 2019, Ghosh et al., 2014, Palacios et al., 2004)
    tsu - Pym
    x-ray crystallography, anti tag coimmunoprecipitation, western blot, experimental knowledge based, anti bait coimmunoprecipitation, pull down, molecular weight estimation by staining
    (Ghosh et al., 2014, Guruharsha et al., 2011, Bono et al., 2004)
    tsu - cdm
    x-ray crystallography, pull down, molecular weight estimation by staining
    (Bono et al., 2010)
    tsu - mago
    anti tag coimmunoprecipitation, western blot, pull down, molecular weight estimation by staining, molecular sieving, anti tag western blot, experimental knowledge based, x-ray crystallography
    (Obrdlik et al., 2019, Ghosh et al., 2014, Guruharsha et al., 2011, Bono et al., 2010, Lewandowski et al., 2010, Bono et al., 2004, Fribourg et al., 2003, Shi and Xu, 2003)
    Summary of Genetic Interactions
    Interaction Browsers
    View in FlyBase Interactions Browser   View in MIST tool (external link)
    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Cln3
    enhanceable
    tsu
    (Tuxworth et al., 2009)
    mbl
    enhanceable
    tsu
    (Vicente-Crespo et al., 2008)
    Ras85D
    suppressible
    tsu
    (Ashton-Beaucage et al., 2010)
    External Data
    Subunit Structure (UniProtKB)
    Heterodimer (via RRM domain) with mago (PubMed:11691839, PubMed:12704080, PubMed:12730685). Part of the mRNA splicing-dependent exon junction complex (EJC) complex; the core complex contains btz/CASC3, eIF4AIII, mago and tsu/RBM8A (PubMed:14973490). Interacts with Pym (via N-terminus); the interaction is direct (PubMed:14968132, PubMed:24967911). Interacts with eIF4AIII (PubMed:14973490).
    (UniProt, Q9V535 )
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    FlyBase
    External Links
    External Data
    Linkouts
    KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Class of Gene
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2R
    Recombination map
    2-60
    Cytogenetic map
    45A7-45A7
    Sequence location
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    45A7-45A7
    Limits computationally determined from genome sequence between P{lacW}l(2)45Aik00116&P{PZ}Su(var)2-1003697 and P{lacW}CG8788k04512&P{lacW}l(2)k13412k13412
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    45A-45A
    (Mohr, 1999.7.30)
    45A4-45A4
    (determined by in situ hybridisation)
    (Mohr et al., 2001)
    Experimentally Determined Recombination Data
    Location

    2-60

    (FlyBase, 2016)

    2-58.1

    (Comeron et al., 2012)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (10)
    Genomic Clones (13)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (124)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
    Other clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    Antibody Information
    Laboratory Generated Antibodies
     

    monoclonal

    (Na et al., 2016)

    polyclonal

    (Choudhury et al., 2016)
    Commercially Available Antibodies
     
    Cell Line Information
    Publicly Available Cell Lines
     
      Other Stable Cell Lines
       
        Other Comments

        tsu is required to restrict the oocyte fate to a single cell during oogenesis.

        (Parma et al., 2007)

        The mago/tsu physical interaction is conserved, occurs in vitro and in vivo and is not RNA mediated. mago and tsu gene products localize within nuclear speckles. mago/tsu heterodimers associate with spliced mRNAs carrying an exon-exon junction complex.

        (Le Hir et al., 2001)

        mago and tsu are required for cell viability.

        (Le Hir et al., 2001)
        Relationship to Other Genes
        Source for database merge of
        Additional comments

        Source for identity of tsu CG8781 was sequence comparison ( date:011219 ).

        (FlyBase, 1996-)
        Nomenclature History
        Source for database identify of

        Source for identity of: tsu CG8781

        (FlyBase, 1996-)
        Nomenclature comments
        Etymology

        Gene is named "tsunagi", which is Japanese for "connection" or "link".

        (Mohr et al., 2001)
        Synonyms and Secondary IDs (11)
        Reported As
        Symbol Synonym
        CG8781
        (Na et al., 2016, Brooks et al., 2015, Hosono et al., 2015, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Herold et al., 2009, Keleman et al., 2009.8.5, Perkins et al., 2009, Gandhi et al., 2007, Gatfield et al., 2003, Gatfield and Izaurralde, 2002)
        TSU
        (Sofola et al., 2007)
        Tsu
        (Lewandowski et al., 2010)
        Y14
        (Bayer et al., 2024, Verma et al., 2023, Haussmann et al., 2022, Lu and Guo, 2020, Obrdlik et al., 2019, Singh et al., 2019, Trcek and Lehmann, 2019, Garcia-Garcia et al., 2017, Choudhury et al., 2016, Ghosh et al., 2012, Bono et al., 2010, Lewandowski et al., 2010, Roignant and Treisman, 2010, Saulière et al., 2010, Roignant et al., 2007, Behm-Ansmant and Izaurralde, 2006, Minakhina and Steward, 2005, Bono et al., 2004, Forler et al., 2004, Hachet and Ephrussi, 2004, Huynh et al., 2004, Palacios et al., 2004, Buscaino et al., 2003, Fribourg et al., 2003, Gatfield et al., 2003, Hall, 2003, Le Hir et al., 2003, Shi and Xu, 2003, Gatfield and Izaurralde, 2002, Hachet and Ephrussi, 2002, Palacios, 2002, Tekotte and Davis, 2002, Le Hir et al., 2001)
        Y14/Tsunagi
        (Hachet and Ephrussi, 2004, Wilkie et al., 2003)
        cg8781
        (Lasko, 2000)
        dY14
        (Wu et al., 2019)
        tsu
        (Miao et al., 2022, Joseph and Lai, 2021, Gao et al., 2019, Cho et al., 2018, Wang et al., 2018, Ashton-Beaucage and Therrien, 2017, Na et al., 2016, Brooks et al., 2015, Li et al., 2015, Yan and Perrimon, 2015, Hayashi et al., 2014, Malone et al., 2014, Czech et al., 2013, Handler et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Ashton-Beaucage and Therrien, 2011, Couthouis et al., 2011, Neumüller et al., 2011, Ashton-Beaucage et al., 2010, Roignant and Treisman, 2010, Dworkin et al., 2009, Herold et al., 2009, Tuxworth et al., 2009, Garcia-Lopez et al., 2008, Vicente-Crespo et al., 2008, Christensen and Cook, 2007.3.22, Parma et al., 2007, Sofola et al., 2007, Liebl et al., 2006)
        y14
        (Ghosh et al., 2014, Besse et al., 2009, Rehwinkel et al., 2004, Herold et al., 2003, Anonymous, 2001, Hachet and Ephrussi, 2001)
        Name Synonyms
        Tsunagi
        (Lewandowski et al., 2010, Nie et al., 2009, Tuxworth et al., 2009, Okamura et al., 2008)
        Y14
        (Tuxworth et al., 2009)
        tsunagi
        (Marie et al., 2017, Jakšić and Schlötterer, 2016, Rumpf et al., 2014, Handler et al., 2013, Tuxworth et al., 2009, Garcia-Lopez et al., 2008, Vicente-Crespo et al., 2008, Parma et al., 2007, Mohr et al., 2001)
        Secondary FlyBase IDs
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          Study result (0)
          Result
          Result Type
          Title
          External Crossreferences and Linkouts ( 49 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
          UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
          Other crossreferences
          AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
          BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
          DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
          EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
          FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
          FlyMine - An integrated database for Drosophila genomics
          InterPro - A database of protein families, domains and functional sites
          KEGG Genes - Molecular building blocks of life in the genomic space.
          MARRVEL_MODEL - MARRVEL (model organism gene)
          PDB - An information portal to biological macromolecular structures
          Linkouts
          BioGRID - A database of protein and genetic interactions.
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
          DroID - A comprehensive database of gene and protein interactions.
          DRSC - Results frm RNAi screens
          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
          Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
          Flygut - An atlas of the Drosophila adult midgut
          FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
          iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
          KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
          MIST (protein-protein) - An integrated Molecular Interaction Database
          Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
          References (126)