FlyBase Gene Report: Dmel\Lsd-2

General Information

Symbol

Dmel\Lsd-2

Species

D. melanogaster

Name

Lipid storage droplet-2

Annotation Symbol

CG9057

Feature Type

protein_coding_gene

FlyBase ID

FBgn0030608

Gene Model Status

Current

Stock Availability

19 publicly available

Gene Summary

Lipid storage droplet-2 (Lsd-2) encodes a protein associated with lipid droplets. It acts as a barrier for lipases (such as the product of bmm) and thus prevents the mobilization of lipid stores. It is involved in regulation of lipid storage amount and energy homeostasis and acts in concert with the product of Lsd-1. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
UniProtKB
Interactive Fly

Also Known As

LSD2, plin2, LSD, Perilipin2

Key Links

Genomic Location

Cytogenetic map

13A8-13A9

Sequence location

X:15,069,127..15,076,220 [-]

Recombination map

1-49

RefSeq locus

NC_004354 REGION:15069127..15076220

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (12 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with FLYBASE:klar; FB:FBgn0001316

(Welte et al., 2005)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (9 terms)

Terms Based on Experimental Evidence (9 terms)

CV Term

Evidence

References

involved_in lipid droplet formation

inferred from genetic interaction with FLYBASE:mdy; FB:FBgn0004797

(Zhao et al., 2022)

involved_in lipid droplet organization

inferred from mutant phenotype

(Ugrankar et al., 2019)

involved_in lipid droplet transport along microtubule

inferred from mutant phenotype

(Welte et al., 2005)

involved_in lipid storage

inferred from mutant phenotype

(Vereshchagina and Wilson, 2006, Gronke et al., 2003)

inferred from expression pattern

(Teixeira et al., 2003)

inferred from expression pattern

(Gronke et al., 2003)

involved_in lipid transport

inferred from expression pattern

(Gronke et al., 2003, Teixeira et al., 2003)

involved_in negative regulation of lipid catabolic process

inferred from mutant phenotype

(Zhao et al., 2022, Bi et al., 2012)

involved_in positive regulation of triglyceride storage

inferred from genetic interaction with FLYBASE:bmm; FB:FBgn0036449

(Gronke et al., 2005)

involved_in regulation of transport

inferred from mutant phenotype

(Welte et al., 2005)

involved_in triglyceride storage

inferred from mutant phenotype

(Gutierrez et al., 2007, Teixeira et al., 2003)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in lipid storage

inferred from biological aspect of ancestor with PANTHER:PTN004450669

(GO Reference Genome Project, 2011-)

involved_in positive regulation of triglyceride storage

inferred from biological aspect of ancestor with PANTHER:PTN004450669

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in cytosol

inferred from direct assay

(Vereshchagina and Wilson, 2006)

is_active_in lipid droplet

inferred from direct assay

(Liu et al., 2024, Ueda et al., 2022, Zhao et al., 2022, Bi et al., 2012, Welte et al., 2005)

located_in lipid droplet

inferred from direct assay

(Gronke et al., 2003, Miura et al., 2002)

inferred from direct assay

(Teixeira et al., 2003)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in cytosol

inferred from biological aspect of ancestor with PANTHER:PTN004450669

(GO Reference Genome Project, 2011-)

is_active_in lipid droplet

inferred from biological aspect of ancestor with PANTHER:PTN004450669

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Protein Family (UniProt)

Belongs to the perilipin family. (Q9VXY7)

Protein Signatures (InterPro)

Perilipin

Summaries

Gene Snapshot

Protein Function (UniProtKB)

Essential for embryogenesis. Required for normal deposition of neutral lipids in the oocyte.

(UniProt, Q9VXY7)

Summary (Interactive Fly)

intracellular neutral lipid droplet storage protein - maintains energy homeostasis

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Lsd-2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VXY7)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.50

Multiphase exon postulated: exon reading frame differs in alternative transcripts; overlap >20aa.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.56

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Lsd-2-RA

FBtr0073967

NM_132768

2148

352

Lsd-2-RB

FBtr0110968

NM_001042811

2097

335

Lsd-2-RD

FBtr0340427

NM_001042812

2279

318

Lsd-2-RE

FBtr0340428

NM_001298332

2330

335

Lsd-2-RF

FBtr0346060

NM_001298333

4598

335

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Lsd-2-PA

FBpp0073784

38.2

352

8.72

FBpp0110268

36.4

335

9.20

FBpp0309373

35.6

318

6.17

FBpp0309374

37.4

335

5.54

FBpp0311908

37.4

335

5.54

X2JKC1

NP_001285262

AHN59732

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

335 aa isoforms: Lsd-2-PE, Lsd-2-PF

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Perilipin

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Lsd-2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Lsd-2_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.28

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4

organism | ubiquitous

(Gronke et al., 2003)

embryonic stage 4 -- 6

organism

Comment: rapidly degraded

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

primordial germ cell

(Fisher et al., 2012)

pole plasm

(Fisher et al., 2012)

embryonic stage 5

germline cell

(Gronke et al., 2003)

embryonic stage 5 -- 11

germline cell

(Gronke et al., 2003)

embryonic stage 7 -- 8

primordial germ cell

(Fisher et al., 2012)

embryonic stage 9 -- 10

germline cell

(Fisher et al., 2012)

embryonic stage 11 -- 12

fat body/gonad primordium

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

embryonic stage 12 -- 17

embryonic/larval fat body

(Gronke et al., 2003)

embryonic/larval midgut primordium

(Gronke et al., 2003)

embryonic stage 13

amnioserosa

(Gronke et al., 2003)

embryonic stage 13 -- 16

embryonic/larval fat body

(Fisher et al., 2012)

germline cell

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

late embryonic stage

embryonic/larval midgut

(Gronke et al., 2003)

third instar larval stage

fat body

(Gronke et al., 2003)

wandering third instar larval stage

wing disc | restricted

(Fauny et al., 2005)

wing pouch | restricted

(Fauny et al., 2005)

anterior-posterior compartment boundary of the wing disc of wing pouch

(Fauny et al., 2005)

mesothoracic tergum primordium

(Fauny et al., 2005)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

organism

(Gronke et al., 2003)

Additional Descriptive Data

Lsd-2 transcript is expressed in the wing pouch of wandering third instar larvae; expression is more pronounced in a strong band with a weaker juxtaposed band along the anterior-posterior compartment boundary, and excluded from the dorsal-ventral compartment boundary. Lsd-2 transcript is also expressed in the presumptive notum.

(Fauny et al., 2005)

Maternally contributed Lsd-2 transcript is detected ubiquitously in pre-cellular blastoderm embryos. In cellular blastoderm the transcript is only detected in germ cells. During germ band contraction expression is detected in the amnioserosa and anterior of the midgut as well as the fat body. During third instar Lsd-2 is detected exclusively in the fat body.

(Gronke et al., 2003)

Northern analysis demonstrates that Lsd-2 transcript levels are highest in early embryos (0-3 hours AEL), third instar larvae, and adults.

(Gronke et al., 2003)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

distribution deduced from reporter or direct label

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

fat body

• lipid droplet

(Gronke et al., 2003)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

wing disc | restricted

(Fauny et al., 2005)

wing pouch | restricted

(Fauny et al., 2005)

anterior-posterior compartment boundary of the wing disc of wing pouch

(Fauny et al., 2005)

mesothoracic tergum primordium

(Fauny et al., 2005)

imaginal disc epithelial cell of wing disc

(Fauny et al., 2005)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Lsd-2 protein is expressed in the wing pouch of wandering third instar larvae; expression is excluded from the dorsal-ventral compartment boundary. Lsd-2 protein is also expressed in the presumptive notum. Lsd-2 protein is restricted to the epithelial cell layer of the wing disc, and does not colocalize with adepithelial-expressed twi protein.

(Fauny et al., 2005)

A GFP-Lsd-2 fusion protein was used to determine that Lsd-2 protein was associated with fat droplets and vesicles in the third instar larval fat body.

(Gronke et al., 2003)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytosol

inferred from direct assay

(Vereshchagina and Wilson, 2006)

is_active_in lipid droplet

inferred from direct assay

(Liu et al., 2024, Ueda et al., 2022, Zhao et al., 2022, Bi et al., 2012, Welte et al., 2005)

located_in lipid droplet

inferred from direct assay

(Gronke et al., 2003, Miura et al., 2002)

inferred from direct assay

(Teixeira et al., 2003)

Expression Deduced from Reporters

Reporter: PBac{681.P.FSVS-1}Lsd-2^CPTI001655

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain cell body rind

(Knowles-Barley, 2011.8.24)

adult gnathal ganglion

(Knowles-Barley, 2011.8.24)

Reporter: PBac{681.P.FSVS-1}Lsd-2^CPTI002546

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain

(Knowles-Barley, 2011.8.24)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Lsd-2 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Lsd-2

View exonic expression by tissue for Dmel\Lsd-2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0030608

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32437/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0030608

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0030608

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0030608

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9057

Flygut - An atlas of the Drosophila adult midgut

FBgn0030608

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Lsd-2

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 22 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 14 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Lsd-2

Transgenic constructs containing regulatory region of Lsd-2

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(1)Exel6249

(Ryder, 2004.4.26)

Inferred from location ( 13 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die during embryonic stage | non-rescuable maternal effect

Lsd-2¹

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Lsd-2^GD14108

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Lsd-2^GD14108

viable, with Scer\GAL4^Mef2.PR

Lsd-2^GD14108

viable, with Scer\GAL4^tin.CΔ4

Lsd-2^GD14108

Sterility

Allele

fertile

Other Phenotypes

Allele

decreased fecundity

Lsd-2^KG00149

decreased size, with Scer\GAL4^Cg.PU

Lsd-2^HMS00629

flightless, with Scer\GAL4^Mef2.PR

Lsd-2^GD14108

increased cell death | third instar larval stage, with Scer\GAL4^Bx-MS1096-KE

some die during embryonic stage | non-rescuable maternal effect

Lsd-2¹

some die during pupal stage, with Scer\GAL4^pnr-MD237

Lsd-2^GD14108

visible, with Scer\GAL4^pnr-MD237

Lsd-2^GD14108

visible | adult stage, with Scer\GAL4^Bx-MS1096-KE

Phenotype manifest in

Allele

brain | larval stage, with Scer\GAL4^repo

Lsd-2^KK111306

embryonic/larval fat body | larval stage

Lsd-2^KG00149

embryonic/larval oenocyte | larval stage | nutrition conditional, with Scer\GAL4^Desat1.PB

Lsd-2^UAS.cGa

fat body

fat body, with Scer\GAL4^Adh.PF

Lsd-2^UAS.cGa

fat body, with Scer\GAL4^fat

Lsd-2^UAS.cGa

fat cell | third instar larval stage, with Scer\GAL4^DcG

Lsd-2^HMS00629

lipid droplet

Lsd-2^KG00149

lipid droplet, with Scer\GAL4^Cg.PU

Lsd-2^HMS00629

lipid droplet & larval oenocyte, with Scer\GAL4^ppl.PP

Lsd-2^UAS.cGa

lipid droplet | adult stage

Lsd-2^KG00149

lipid droplet | adult stage, with Scer\GAL4^Mhc.PK

Lsd-2^UAS.cUa

lipid droplet | adult stage | increased number, with Scer\GAL4^ninaE.PT

Lsd-2^UAS.EGFP

lipid droplet | adult stage | progressive, with Scer\GAL4^Mhc.PK

Lsd-2^HMS00629

lipid droplet | embryonic stage

lipid droplet | embryonic stage | maternal effect

Lsd-2^KG00149

lipid droplet | larval stage, with Scer\GAL4^repo

Lsd-2^KK111306

lipid droplet | third instar larval stage, with Scer\GAL4^DcG

Lsd-2^HMS00629

mesonotum, with Scer\GAL4^pnr-MD237

Lsd-2^GD14108

mesothoracic tergotrochanter muscle cell

Lsd-2^KG00149

mesothoracic tergotrochanter muscle cell, with Scer\GAL4^Mhc.PK

Lsd-2^UAS.cUa

mesothoracic tergotrochanter muscle cell | progressive, with Scer\GAL4^Mhc.PK

Lsd-2^HMS00629

myofibril, with Scer\GAL4^Mef2.PR

Lsd-2^GD14108

nurse cell

Lsd-2¹

nurse cell (with Lsd-2¹)

Lsd-2^KG00149

nurse cell (with Lsd-2^KG00149)

Lsd-2¹

photoreceptor neuron | adult stage, with Scer\GAL4^ninaE.PT

Lsd-2^UAS.EGFP

sarcomere, with Scer\GAL4^Mef2.PR

Lsd-2^GD14108

wing, with Scer\GAL4^Bx-MS1096-KE

wing pouch | third instar larval stage, with Scer\GAL4^Bx-MS1096-KE

Z disc, with Scer\GAL4^Mef2.PR

Lsd-2^GD14108

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (5)

Hsap\PLIN2

7 of 14

Yes

Hsap\PLIN1

5 of 14

Yes

Hsap\PLIN3

5 of 14

Yes

Hsap\PLIN5

5 of 14

Yes

Hsap\PLIN4

2 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (5)

Rnor\Plin2

7 of 14

Yes

Rnor\Plin3

7 of 14

Yes

Rnor\Plin1

6 of 14

Yes

Rnor\Plin5

6 of 14

Yes

Rnor\Plin4

1 of 14

Mus musculus (laboratory mouse) (5)

Mmus\Plin5

8 of 14

Yes

Mmus\Plin3

7 of 14

Yes

Mmus\Plin1

6 of 14

Yes

Mmus\Plin2

6 of 14

Yes

Mmus\Plin4

2 of 14

Xenopus tropicalis (Western clawed frog) (5)

Xtro\plin3

7 of 13

Yes

Xtro\plin2

5 of 13

Xtro\LOC101734278

2 of 13

Yes

Xtro\plin1

2 of 13

Xtro\samd8

1 of 13

Danio rerio (Zebrafish) (5)

Drer\plin2

8 of 14

Yes

Drer\plin1

6 of 14

Yes

Drer\zgc:77486

5 of 14

Yes

Drer\plin6

4 of 14

Yes

Drer\plin3

2 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (2)

Agam\AgaP_AGAP000167

11 of 12

Yes

Agam\AgaP_AGAP002890

2 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Lsd-2. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (1)

Lsd-1

4 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 3 )

Allele

Disease

Evidence

References

Lsd-2^HMS00629

model of metabolic dysfunction-associated steatotic liver disease

CEA

(Zhang et al., 2022)

Lsd-2^UAS.cGa

model of obesity

CEA

(Gronke et al., 2003)

Lsd-2^UAS.EYFP

model of obesity

CEA

(Yan et al., 2017)

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

PLIN1; perilipin 1

model of familial partial lipodystrophy type 4

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 5 )

Allele

Disease

Interaction

References

Lsd-2^HMS00629

ameliorates obesity

modeled by HDAC6^KO

(Yan et al., 2017)

Lsd-2^KG00149

ameliorates obesity

modeled by HDAC6^KO

(Yan et al., 2017)

Lsd-2^UAS.EGFP

exacerbates Parkinson's disease 1

modeled by Hsap\SNCA^UAS.cFa

(Girard et al., 2021)

Lsd-2^UAS.cGa

ameliorates neurodegeneration with brain iron accumulation 4

modeled by Nazo^KO

(Sreejith et al., 2024)

Lsd-2^UAS.EYFP

model of obesity

is ameliorated by HDAC6^UAS.cDa

(Yan et al., 2017)

is ameliorated by Hsc70-4^UAS.cEa

(Yan et al., 2017)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

7 of 14

5 of 14

LIPODYSTROPHY, FAMILIAL PARTIAL, TYPE 4; FPLD4

familial partial lipodystrophy type 4

5 of 14

5 of 14

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Lsd-2 - CG11700

split luciferase complementation, luminiscence technology

(Kolkhof et al., 2017)

Lsd-2 - Hsap\SNCA

colocalization, fluorescence microscopy, inferred by author

(Girard et al., 2021)

Lsd-2 - Hsc70-4

anti tag coimmunoprecipitation, western blot

(Yan et al., 2017)

Lsd-2 - Rdh1

anti tag coimmunoprecipitation, anti tag western blot

(Zhao et al., 2022)

Lsd-2 - Ubi-p5E

split luciferase complementation, luminiscence technology

(Kolkhof et al., 2017)

Lsd-2 - Ubi-p63E

split luciferase complementation, luminiscence technology

(Kolkhof et al., 2017)

Lsd-2 - Ubr1

pull down, western blot, anti tag western blot, anti tag coimmunoprecipitation, enzymatic study

(Zhao et al., 2023, Zhang et al., 2022)

Lsd-2 - Ugt35B1

anti tag coimmunoprecipitation, Identification by mass spectrometry, anti tag western blot

(Sheng et al., 2025)

Lsd-2 - klar

two hybrid

(Welte et al., 2005)

Lsd-2 - sturkopf

split luciferase complementation, luminiscence technology

(Kolkhof et al., 2017)

Lsd-2 - α-Est7

split luciferase complementation, luminiscence technology

(Kolkhof et al., 2017)

RNA-protein

Physical Interaction

Assay

References

Lsd-2 - aub

iclip, partial RNA sequence identification

(Barckmann et al., 2015)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Lsd-2

suppressible

bmm

(Gronke et al., 2005)

Lsd-2

suppressible

Diap1

(Binh et al., 2019)

Lsd-2

suppressible

foxo

(Binh et al., 2019)

Lsd-2

enhanceable

foxo

(Binh et al., 2019)

suppressible

(Yan et al., 2017)

suppressible

(Yan et al., 2017)

enhanceable

(Bi et al., 2012)

suppressible

(Bi et al., 2012)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

babo

suppressible

Lsd-2

(Sheng et al., 2025)

bmm

suppressible

Lsd-2

(Gronke et al., 2005)

HDAC6

suppressible

Lsd-2

(Yan et al., 2017)

suppressible

Lsd-2

(Dong et al., 2021)

htl

suppressible

Lsd-2

(Dong et al., 2021)

enhanceable

(Bi et al., 2012)

suppressible

(Sreejith et al., 2024)

Nazo|CG3740

suppressible

Lsd-2

(Sreejith et al., 2024)

Rdh1

suppressible

Lsd-2

(Zhao et al., 2022)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

58792

DroID - A comprehensive database of gene and protein interactions.

FBgn0030608

MIST (genetic) - An integrated Molecular Interaction Database

32437

MIST (protein-protein) - An integrated Molecular Interaction Database

32437

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-49

Cytogenetic map

13A8-13A9

Sequence location

X:15,069,127..15,076,220 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

13A8-13A9

Limits computationally determined from genome sequence between P{EP}hiw^EP1308 and P{EP}Ahcy13^EP1007

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-49

(FlyBase, 2016)

1-49.7

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (19)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

605258

w^* Lsd-2¹

98116

w^*; P{UAS-Lsd-2.G}2

98125

y¹ Lsd-2⁵¹/FM7i

12398

w¹¹¹⁸ P{GT1}Lsd-2^BG00016

19733

y¹ w^67c23 P{EPgy2}EY07971

55471

y¹ w^* Mi{MIC}Lsd-2^MI10352

Korea

10594

P{EP}Lsd-2^G15

Kyoto

112057

w^* P{GawB}Lsd-2^NP0141 / FM7c

115181

w¹¹¹⁸ PBac{681.P.FSVS-1}Lsd-2^CPTI001655

115301

w¹¹¹⁸ PBac{681.P.FSVS-1}Lsd-2^CPTI002546

VDRC

v102269

P{KK111306}VIE-260B

v40734

w¹¹¹⁸; P{GD14108}v40734

More stocks available...

Genomic Clones (9)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (217)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH06230

Other clones

DPiM_GH06230

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0030608

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0030608

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Giedt et al., 2023)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Lsd-2 lack-of-function and gain-of-function studies indicate that the gene is essential for an adjustment of the organismal lipid storage level.

(Beller et al., 2003)

Lsd-2 is required for normal lipid storage.

(Teixeira et al., 2003)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Lsd2 CG9057

(Beller, 2002.3.17)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (26)

Reported As

Symbol Synonym

145098_at

(Kristensen et al., 2005)

CG9057

(Zhao et al., 2022, Hosono et al., 2015, Jaspers et al., 2012, Cammarato et al., 2011, Kühnlein, 2011, Ni et al., 2010.12.1, Ellis and Carney, 2009, Keleman et al., 2009.8.5, Arrese et al., 2008, Hughes et al., 2008, Beller et al., 2006, Cermelli et al., 2006, Bartolome and Maside, 2004, Butler et al., 2003)

DmPLIN2

(Lee et al., 2013)

LSD

(Wang et al., 2022, Westfall et al., 2019)

LSD-2

(Stephenson et al., 2021, Franchet et al., 2019, Arora et al., 2016, Li et al., 2012, Li et al., 2012, Beller et al., 2010, Pospisilik et al., 2010, Miura et al., 2002)

LSP-2

(Parisi et al., 2013)

Lsd-2

(Sheng et al., 2025, Kim et al., 2024, Liu et al., 2024, Nguyen et al., 2024, Floc'hlay et al., 2023, Lu et al., 2023, Zhang et al., 2023, Binh et al., 2022, Kharrat et al., 2022, Pasquier and Robichon, 2022, Parra-Peralbo et al., 2021, Cho et al., 2020, Lee et al., 2020, Thanh et al., 2020, Wat et al., 2020, Binh et al., 2019, Liu et al., 2019, Gene Disruption Project members, 2018-, Kolkhof et al., 2017, Paiardi et al., 2017, Tschapalda et al., 2016, Ugur et al., 2016, Barckmann et al., 2015, Fear et al., 2015, Sieber and Spradling, 2015, Tsuda-Sakurai et al., 2015, Ashwal-Fluss et al., 2014, DeSalvo et al., 2014, Lye et al., 2014, Rajan and Perrimon, 2013, Bolukbasi et al., 2012, Linford et al., 2012, Carreira et al., 2011, Tian et al., 2011, Yu et al., 2011, Kühnlein, 2010, Carreira et al., 2009, Ellis and Carney, 2009, Morozova et al., 2009, Sieber and Thummel, 2009, Buszczak et al., 2007, Morozova et al., 2007, Weake and Scott, 2007, Beller et al., 2006, Beller et al., 2006, Fauny et al., 2005, Gronke et al., 2005)

Lsd2

(He et al., 2022, Moraes and Montagne, 2021, Benoit et al., 2020, Lee et al., 2019, Cinnamon et al., 2016, Zhang et al., 2011, Thimgan et al., 2010, Arrese et al., 2008, Gutierrez et al., 2007, Skorupa et al., 2007, Greil et al., 2003, Gronke, 2003, Gronke et al., 2003, Teixeira et al., 2003, Beller, 2002.3.17)

PLIN2

(Heier et al., 2021, Toprak et al., 2020, Musselman and Kühnlein, 2018, Zheng et al., 2016, Thiel et al., 2013, Kuhnlein, 2012)

Plin2

(Zhao et al., 2023, Zhao et al., 2022, Yan et al., 2017)

dPlin2

(Girard et al., 2021)

dmPLIN2

(Kolkhof et al., 2017)

lsd-2

(De Groef et al., 2024, Harsh et al., 2019)

lsd2

(Dark et al., 2024, Li et al., 2023, Dong et al., 2021, Yurgel et al., 2015, Potdar and Sheeba, 2013)

plin2

(Pardo-Garcia et al., 2023, Zhang et al., 2022, Melentev et al., 2021, Kierdorf et al., 2020, May et al., 2019, Cabirol-Pol et al., 2018, Bi et al., 2012, Wang et al., 2012, Beller et al., 2010, Beller et al., 2010)

Name Synonyms

Lipid Storage Droplet 2 Gene

(Fauny et al., 2005)

Lipid Storage Droplet protein 2

(Brasaemle and Hansen, 2006)

Lipid storage droplet 2

(Ugur et al., 2016, Thimgan et al., 2010, Lai et al., 2007, Rollmann et al., 2007)

Lipid storage droplet-2

(Shafer, 2025, Jaspers et al., 2012, Cammarato et al., 2011, Kühnlein, 2011, Kühnlein, 2010, Morozova et al., 2007, Miura et al., 2002)

Lipid-storage Droplet-2

(Dissel et al., 2015)

Perilipin2

(Sreejith et al., 2024, Parisi et al., 2013)

lipid storage droplet 2

(Yurgel et al., 2015, Cermelli et al., 2006)

lipid storage droplet-2

(Carreira et al., 2009)

perilipin

(Lee et al., 2013)

perilipin-2

(Cabirol-Pol et al., 2018)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 55 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

32437

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VXY7

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VXY7

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VXY7

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0030608

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32437/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0030608

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0030608

FlyMine - An integrated database for Drosophila genomics

FBgn0030608

InterPro - A database of protein families, domains and functional sites

Perilipin

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG9057

MARRVEL_MODEL - MARRVEL (model organism gene)

32437

Linkouts

BioGRID - A database of protein and genetic interactions.

58792

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0030608

DroID - A comprehensive database of gene and protein interactions.

FBgn0030608

DRSC - Results frm RNAi screens

FBgn0030608

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Lsd-2_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0030608

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0030608

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9057

Flygut - An atlas of the Drosophila adult midgut

FBgn0030608

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0030608

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

32437

MIST (protein-protein) - An integrated Molecular Interaction Database

32437

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (200)

The following external sites may use different assemblies or annotations than FlyBase.

-1.28

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