FlyBase Allele Report: Scer\GAL4[sr-md710]

General Information

Symbol

Scer\GAL4^sr-md710

Species

S. cerevisiae

Name

FlyBase ID

FBal0120626

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

P{GawB}sr^md710

Carried in Construct

Also Known As

sr-GAL4, Stripe-Gal4, srGAL4, sr.Gal4, sr^md710, Sr-Gal4^md710

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{GawB}sr^md710

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{GawB}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:18,121,952..18,121,952 [+]

(Pissarek et al., 2025, Yoshino et al., 2025, Luedke et al., 2024, Moucaud et al., 2024, Zhang et al., 2024, Liu et al., 2023, Suver et al., 2023, Takayanagi-Kiya et al., 2023, Chu and Hayashi, 2021, Coni et al., 2021, Bataillé et al., 2020, Bruels et al., 2019, Laddada et al., 2019, Lemke et al., 2019, Pérez-Moreno et al., 2017, Ríos-Barrera et al., 2017, Green et al., 2016, Hudry et al., 2016, Saavedra et al., 2016, Ordan et al., 2015, Tiwari et al., 2015, Wang et al., 2015, Boukhatmi et al., 2014, Butí et al., 2014, Saavedra et al., 2014, Schulman et al., 2014, Bharadwaj et al., 2013, Liu et al., 2013, Uchino et al., 2013, Alves-Silva et al., 2012, Krzemien et al., 2012, Lahaye et al., 2012, Nir et al., 2012, Gilsohn and Volk, 2010, Bottenberg et al., 2009, Shalaby et al., 2009, Wayburn and Volk, 2009, Ikmi et al., 2008, Loer et al., 2008, Loer et al., 2008, Schnorrer et al., 2007, Subramanian et al., 2007, van der Plas et al., 2007, Maqbool et al., 2006, Usui-Ishihara and Simpson, 2005, Inbal et al., 2004, Soler et al., 2004, Soustelle et al., 2004, Swan et al., 2004, Usui et al., 2004, Subramanian et al., 2003, Dorfman et al., 2002, Nabel-Rosen et al., 2002, Ghazi, 2001.1.22, Ghazi et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

prepupal stage P3

tendon cell

(Laddada et al., 2019)

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

tendon cell

(Swan et al., 2004)

chordotonal organ | subset

(Inbal et al., 2004)

lch5 cap attachment cell

(Inbal et al., 2004)

embryonic stage 16

scolopidial ligament cell | subset

(Inbal et al., 2004)

first instar larval stage -- second instar larval stage

tendon cell

(Subramanian et al., 2003)

third instar larval stage

wing disc | restricted

(Usui et al., 2004)

scutum | presumptive

(Usui et al., 2004)

tendon cell | precursor

(Soler et al., 2004)

leg disc

(Soler et al., 2004)

tendon cell

(Laddada et al., 2019)

embryonic/larval tendon cell

(Liu et al., 2023)

pupal stage

mesonotum | restricted

(Usui et al., 2004)

pupal stage P6 -- pharate adult stage P15

tendon cell

Comment: reference states >=26 hr APF

(Ghazi et al., 2000)

adult stage

tendon cell

(Krzemien et al., 2012)

Additional Information

Statement

Reference

To study tendon development, sr reporter expression was studied during leg development relative to and odd reporter, which is expressed at the segmental joints. The first tendon to develop is the long tendon of the tarsi that arises from cells coexpressing sr and odd at the T5/pretarsus boundary at early third instar. These cells invaginate and elongate into the tarsal cavity formed by the progressive evagination of the leg disc at the beginning of metamorphosis. By the end of leg development, they will form a long internal tube extending from pre-tarsus to femur with the apical pole facing the lumen. With a short delay, another cluster of tendon precursor cells is specified during the third instar at the femur/tibia boundary in the dorsal position. Subsequently, during early pupation, additional sr-positive clusters are specified along the five segmental pre-patterned odd-positive boundary rings. This indicates that odd/sr-positive tendon precursors are specified progressively following the leg proximodistal segmentation with odd expression preceding sr expression. N was observed to appear sequentially and precede sr expression.

(Laddada et al., 2019)

Drives expression in tendon cells.

(FlyBase Curators, 2017)

Expression is the same as that observed for the sr gene, and does not overlap with the expression pattern of ac or sc.

(Usui et al., 2004)

Larval expression is observed in tendon cells.

(Subramanian et al., 2003)

Pupal expression is observed in the attachment sites of the anterior dorsal medial muscles.

(Ghazi et al., 2000)

Marker for

embryonic/larval tendon cell

(Liu et al., 2023)

tendon cell

(Swan et al., 2004, Usui et al., 2004, Subramanian et al., 2003, Ghazi et al., 2000)

Reflects expression of

(Liu et al., 2023, Laddada et al., 2019, Inbal et al., 2004, Swan et al., 2004, Usui et al., 2004, Subramanian et al., 2003, Ghazi et al., 2000)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight, with Pfdn2^NIG.6302R

(Tiwari et al., 2015)

decreased size, with mys^NIG.1560R

(Tiwari et al., 2015)

decreased size | larval stage, with Tango1^GD956

(Tiwari et al., 2015)

decreased size | P-stage, with Bap60^NIG.4303R

(Tiwari et al., 2015)

decreased size | P-stage, with Caf1-180^NIG.12109R

(Tiwari et al., 2015)

decreased size | P-stage, with CG11417^NIG.11417R

(Tiwari et al., 2015)

decreased size | P-stage, with CG13994^NIG.13994R

(Tiwari et al., 2015)

decreased size | P-stage, with CG30161^NIG.30161R

(Tiwari et al., 2015)

decreased size | P-stage, with Clp^NIG.3642R

(Tiwari et al., 2015)

decreased size | P-stage, with Dhc64C^NIG.7507R

(Tiwari et al., 2015)

decreased size | P-stage, with EcR^NIG.1765R

(Tiwari et al., 2015)

decreased size | P-stage, with Nup160^NIG.4738R

(Tiwari et al., 2015)

decreased size | P-stage, with Ost1^NIG.5364R

(Tiwari et al., 2015)

decreased size | P-stage, with Tango1^GD956

(Tiwari et al., 2015)

decreased size | P-stage, with Tango4^NIG.1796R

(Tiwari et al., 2015)

flightless, with sc^UAS.cHa

(Usui et al., 2004)

lethal, with Tango1^GD956

(Tiwari et al., 2015)

lethal - all die before end of larval stage, with Scer\GAL4^how-24B, shot^RNAi.UAS

(Subramanian et al., 2003)

lethal - all die before end of P-stage, with LamC^NIG.10119R

(Uchino et al., 2013)

lethal - all die during embryonic stage, with mys^GD15002

(Tiwari et al., 2015)

lethal - all die during embryonic stage, with mys^NIG.1560R

(Tiwari et al., 2015)

lethal - all die during embryonic stage, with rhea^GD12050

(Tiwari et al., 2015)

lethal - all die during embryonic stage, with Tango4^NIG.1796R

(Tiwari et al., 2015)

lethal - all die during P-stage, with Bap60^NIG.4303R

(Tiwari et al., 2015)

lethal - all die during P-stage, with Caf1-180^NIG.12109R

(Tiwari et al., 2015)

lethal - all die during P-stage, with CG3124^NIG.3124R

(Tiwari et al., 2015)

lethal - all die during P-stage, with CG11030^NIG.11030R

(Tiwari et al., 2015)

lethal - all die during P-stage, with CG11417^NIG.11417R

(Tiwari et al., 2015)

lethal - all die during P-stage, with CG13994^NIG.13994R

(Tiwari et al., 2015)

lethal - all die during P-stage, with CG30161^NIG.30161R

(Tiwari et al., 2015)

lethal - all die during P-stage, with Clp^NIG.3642R

(Tiwari et al., 2015)

lethal - all die during P-stage, with Dhc64C^NIG.7507R

(Tiwari et al., 2015)

lethal - all die during P-stage, with EcR^NIG.1765R

(Tiwari et al., 2015)

lethal - all die during P-stage, with fipi^NIG.31970R

(Tiwari et al., 2015)

lethal - all die during P-stage, with Nup160^NIG.4738R

(Tiwari et al., 2015)

lethal - all die during P-stage, with Ost1^NIG.5364R

(Tiwari et al., 2015)

lethal - all die during P-stage, with Polr3H^NIG.7339R

(Tiwari et al., 2015)

lethal - all die during P-stage, with Taf2^NIG.6711R

(Tiwari et al., 2015)

lethal - all die during P-stage, with TfIIB^NIG.5193R

(Tiwari et al., 2015)

some die during larval stage, with Scer\GAL4^how-24B, shot^RNAi.UAS

(Subramanian et al., 2003)

some die during P-stage, with LamC^NIG.10119R

(Uchino et al., 2013)

visible, with sc^UAS.cHa

(Usui et al., 2004)

visible, with sr^b.UAS

(Usui et al., 2004)

visible | adult stage, with Rok^KK107802

(Chu and Hayashi, 2021)

Phenotype Manifest In

A1-7 dorsal transverse muscle 1 | larval stage, with form3^HMS00393

(Pissarek et al., 2025)

dorsal longitudinal indirect flight muscle | P-stage, with Bap60^NIG.4303R

(Tiwari et al., 2015)

dorsal longitudinal indirect flight muscle | P-stage, with Caf1-180^NIG.12109R

(Tiwari et al., 2015)

dorsal longitudinal indirect flight muscle | P-stage, with CG11417^NIG.11417R

(Tiwari et al., 2015)

dorsal longitudinal indirect flight muscle | P-stage, with Dhc64C^NIG.7507R

(Tiwari et al., 2015)

dorsal longitudinal indirect flight muscle | P-stage, with EcR^NIG.1765R

(Tiwari et al., 2015)

dorsal longitudinal indirect flight muscle | P-stage, with mys^NIG.1560R

(Tiwari et al., 2015)

dorsal longitudinal indirect flight muscle | P-stage, with Ost1^NIG.5364R

(Tiwari et al., 2015)

dorsal longitudinal indirect flight muscle | P-stage, with Tango1^GD956

(Tiwari et al., 2015)

dorsal longitudinal indirect flight muscle | P-stage, with Tango4^NIG.1796R

(Tiwari et al., 2015)

dorsal longitudinal indirect flight muscle | P-stage | absent, with CG13994^NIG.13994R

(Tiwari et al., 2015)

dorsal longitudinal indirect flight muscle | P-stage | absent, with CG30161^NIG.30161R

(Tiwari et al., 2015)

dorsal longitudinal indirect flight muscle | P-stage | absent, with Clp^NIG.3642R

(Tiwari et al., 2015)

dorsal longitudinal indirect flight muscle | P-stage | absent, with Nup160^NIG.4738R

(Tiwari et al., 2015)

dorsal longitudinal indirect flight muscle cell, with sc^UAS.cHa

(Usui et al., 2004)

embryonic/larval tendon cell | larval stage, with form3^HMS00393

(Pissarek et al., 2025)

embryonic/larval tendon cell | larval stage, with Tango1^GD956

(Tiwari et al., 2015)

exuvial fluid | pupal stage, with Rok^KK107802

(Chu and Hayashi, 2021)

larva, with Tango1^GD956

(Tiwari et al., 2015)

larval somatic muscle cell, with Lrt^UAS.EGFP

(Wayburn and Volk, 2009)

lateral transverse muscle | larval stage, with form3^HMS00393

(Pissarek et al., 2025)

lch5 ligament attachment cell, with Egfr^DN.UAS

(Inbal et al., 2004)

macrochaeta | ectopic, with sc^UAS.cHa

(Usui et al., 2004)

mesothoracic tergum & microchaeta, with sr^b.UAS

(Usui et al., 2004)

microchaeta, with sc^UAS.cHa

(Usui-Ishihara and Simpson, 2005)

microchaeta & mesothoracic tergum, with Amun^GD9779

(Shalaby et al., 2009)

microchaeta & mesothoracic tergum, with Amun^UAS.mRFP1

(Shalaby et al., 2009)

muscle tendon junction | P-stage, with CG11417^NIG.11417R

(Tiwari et al., 2015)

muscle tendon junction | P-stage, with Dhc64C^NIG.7507R

(Tiwari et al., 2015)

muscle tendon junction | P-stage, with mys^NIG.1560R

(Tiwari et al., 2015)

muscle tendon junction | P-stage, with Ost1^NIG.5364R

(Tiwari et al., 2015)

muscle tendon junction | P-stage, with Tango1^GD956

(Tiwari et al., 2015)

muscle tendon junction | P-stage, with Tango4^NIG.1796R

(Tiwari et al., 2015)

somatic muscle cell | P-stage, with LamC^NIG.10119R

(Uchino et al., 2013)

tendon cell, with Scer\GAL4^how-24B, shot^RNAi.UAS

(Subramanian et al., 2003)

tendon cell & actin filament, with Scer\GAL4^how-24B, shot^RNAi.UAS

(Subramanian et al., 2003)

tendon cell & microtubule, with Scer\GAL4^how-24B, shot^RNAi.UAS

(Subramanian et al., 2003)

tendon cell | larval stage, with LamC^NIG.10119R

(Uchino et al., 2013)

tendon cell | P-stage, with CG11417^NIG.11417R

(Tiwari et al., 2015)

tendon cell | P-stage, with CG13994^NIG.13994R

(Tiwari et al., 2015)

tendon cell | P-stage, with CG30161^NIG.30161R

(Tiwari et al., 2015)

tendon cell | P-stage, with Clp^NIG.3642R

(Tiwari et al., 2015)

tendon cell | P-stage, with Dhc64C^NIG.7507R

(Tiwari et al., 2015)

tendon cell | P-stage, with LamC^NIG.10119R

(Uchino et al., 2013)

tendon cell | P-stage, with mys^NIG.1560R

(Tiwari et al., 2015)

tendon cell | P-stage, with Nup160^NIG.4738R

(Tiwari et al., 2015)

tendon cell | P-stage, with Ost1^NIG.5364R

(Tiwari et al., 2015)

tendon cell | P-stage, with Tango1^GD956

(Tiwari et al., 2015)

tendon cell | P-stage, with Tango4^NIG.1796R

(Tiwari et al., 2015)

tendon cell | pupal stage, with Tango1^NIG.11098R

(Ríos-Barrera et al., 2017)

ventral longitudinal muscle cell, with Lrt^UAS.EGFP

(Wayburn and Volk, 2009)

wing, with Rok^KK107802

(Chu and Hayashi, 2021)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^sr-md710/Rok^KK107802 is a suppressor | partially of visible | adult stage phenotype of qsm^F17fs

(Chu and Hayashi, 2021)

Scer\GAL4^sr-md710/Rok^GD1522 is a suppressor | partially of visible | adult stage phenotype of qsm^F17fs

(Chu and Hayashi, 2021)

Phenotype Manifest In

Suppressed by

Statement

Reference

Lrt^UAS.EGFP, Scer\GAL4^sr-md710 has larval somatic muscle cell phenotype, suppressible by robo[+]/robo1¹

(Wayburn and Volk, 2009)

Suppressor of

Statement

Reference

Scer\GAL4^sr-md710/Rok^GD1522 is a suppressor | partially of notum phenotype of qsm^F17fs

(Chu and Hayashi, 2021)

Scer\GAL4^sr-md710/Rok^KK107802 is a suppressor | partially of notum phenotype of qsm^F17fs

(Chu and Hayashi, 2021)

NOT Suppressor of

Statement

Reference

Scer\GAL4^sr-md710/Rok^GD1522 is a non-suppressor of wing phenotype of qsm^F17fs

(Chu and Hayashi, 2021)

Scer\GAL4^sr-md710/Rok^KK107802 is a non-suppressor of wing phenotype of qsm^F17fs

(Chu and Hayashi, 2021)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Partially rescues

Scer\GAL4^sr-md710/qsm^UAS.mCherry partially rescues qsm^F17fs

(Chu and Hayashi, 2021)

Scer\GAL4^sr-md710/LamC^UAS.cSa partially rescues LamC^LC58

(Uchino et al., 2013)

Scer\GAL4^sr-md710/shot^LC.UAS.GFP partially rescues shot^unspecified

(Bottenberg et al., 2009)

Scer\GAL4^sr-md710/shot^{LA-Δrod1.UAS.GFP} partially rescues shot^unspecified

(Bottenberg et al., 2009)

wech^UAS.EGFP, Scer\GAL4^sr-md710, Scer\GAL4^Mef2.PR partially rescues wech⁶⁶

(Loer et al., 2008)

wech^UAS.EGFP/Scer\GAL4^sr-md710 partially rescues wech⁶⁶

(Loer et al., 2008)

wech^UAS.EGFP, Scer\GAL4^sr-md710, Scer\GAL4^Mef2.PR partially rescues wech⁶⁶

(Loer et al., 2008)

Fails to rescue

Scer\GAL4^sr-md710/qsm^{mut.UAS.mCherry} fails to rescue qsm^F17fs

(Chu and Hayashi, 2021)

Scer\GAL4^sr-md710/Ced-12^UAS.cGa fails to rescue Ced-12^KO/Ced-12^19F3

(Liu et al., 2013)

Scer\GAL4^sr-md710/shot^{RE-ΔCtail.UAS.GFP} fails to rescue shot^unspecified

(Alves-Silva et al., 2012)

drl^UAS.cCa/Scer\GAL4^sr-md710 fails to rescue drl^Red2

(Lahaye et al., 2012)

Scer\GAL4^sr-md710/shot^{LA-ΔGAS2.UAS.GFP} fails to rescue shot^unspecified

(Bottenberg et al., 2009)

kon^{UAS.Tag:HA,Tag:SS(wg)}/Scer\GAL4^sr-md710 fails to rescue kon^C452/kon^C25

(Schnorrer et al., 2007)

Scer\GAL4^sr-md710/Grip^UAS.cSa fails to rescue Grip^ex36

(Swan et al., 2004)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

26663

w^*; P{GawB}sr^md710/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Scer\GAL4^sr-md710

sr^GAL4

(Ikmi et al., 2008)

sr^MD710

(Takayanagi-Kiya et al., 2023)

sr^md710

(Saavedra et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (56)

Report Sections

Open Close