Zasp52 was immunostained by using the Z(210) antibody. Zasp52 was found on the Z discs on the myofibrils of internal flight muscles, but not on the myofibrils of the nearby mesothoracic extracoxal depressor muscle 66 (TDT muscle).

At 30 h APF, Zasp52 shows a punctate distribution in the forming myofibrils. At 48 h APF, Zasp52 appears as broad dots at the forming Z-disc. At 72 h APF, Zasp52 is found in evenly spaced stripes at the Z-disc. In the adult, the myofibrils have grown in width and Zasp52 labels the striations. Zasp52 localizes with Actn.

A Zasp52 antibody that recognizes all isoforms is expressed in third instar larval body wall muscle and midgut, as well as in adult indirect flight muscle (IFM), tubular jump muscle (TDT), abdominal muscles, heart muscle, and midgut. Zasp52 localizes to Z-disks in all muscle tissues, heart, visceral, and somatic. Antibodies against exon 8 sequences (encoding one form of the variable LIM1 domain) shows tissue-specific expression. It detects expression in adult heart and certain somatic muscles (IFM and TDT), but not in larval and adult midgut, larval body wall muscles and abdominal muscles.

Z(210) protein is found only in the indirect flight muscle (IFM) and large cells of mesothoracic extracoxal depressor muscle 66 (tergal depressor of trochanter, or jump muscle). It is first detected in the precursors of these cells at 40 hr APF.