Selective ablation of octopamine/tyramine neurons in the brains through co-expression of hidScer\UAS.cUa and rprScer\UAS.cUa under the control of Scer\GAL4Tdc2.PC and VNC-specific Scer\GAL80tsh-GAL80 does not lead to any deficit in appetitive olfactory learning when fructose is given as a reward. Larvae also exhibit robust appetitive memory when sorbitol is used as a reward, comparable to that of genetic controls. In contrast, the larvae fail to show significant learning when rewarded with arabinose; memory performance is significantly reduced compared with controls. Perception of the odors (amyl acetate and benzaldehyde) is unaffected, and larvae show normal attraction behavior toward arabinose and perform indistinguishably from controls.
When embryonic Ilp7 neurons are killed using WScer\UAS.cUa and rprScer\UAS.C (post-embryonic Ilp7 neurons are retained by limiting expression to the first and second instar larval stages using Scer\GAL80ts.αTub84B) female egg laying is normal.
When males express WScer\UAS.cUa and rprScer\UAS.C under the control of Scer\GAL4Ilp7.PC female egg laying is reduced on the first day after eclosion compared to wild type males, but subsequent egg laying is normal.
Females expressing WScer\UAS.cUa and rprScer\UAS.C throughout development under the control of Scer\GAL4Ilp7.PC have severely reduced egg laying. These females also have distended abdomens and eggs are always found jammed in the lateral oviduct. Of the small number of egg laid by these females, only 40% produce viable larvae.
Activity peaks during thermophase/cryophase cycles are virtually eliminated in TrpA11 flies expressing WScer\UAS.cUa under the control of Scer\GAL4cry.PU. When these flies are subsequently allowed to free run at a constant temperature (either 18[o]C or 29[o]C) in constant darkness, only 6% show rhythmic locomotor activity.
Expression of both WScer\UAS.cUa and rprScer\UAS.C under the control of Scer\GAL4Rh5.PT abolishes light avoidance at the larval stage. However, expression under the control of Scer\GAL4Rh6.PD does not affect light avoidance.
Expression of both WScer\UAS.cUa and rprScer\UAS.C efficiently ablates all circadian clock neurons (Scer\GAL4tim.PE), leaving larvae with a seriously disrupted light avoidance phenotype. This phenotype is no different in LD or DD conditions.
hid110 mutants expressing both hidScer\UAS.cUa and rprScer\UAS.cUa in class IV dendritic arborization neurons (under the control of Scer\GAL4ppk.1.9) show markedly decreased white-light-avoidance behavior compared to wild-type and hid110 (bolwig-organ-ablated) mutants.
Expression of both hidScer\UAS.cUa and rprScer\UAS.cUa in class IV dendritic arborization neurons (under the control of Scer\GAL4ppk.1.9) results in marked decrease in white-light-avoidance behavior compared to wild-type.
Expression of Zzzz\E4orf4Scer\UAS.cPa suppresses the semi-viability seen when WScer\UAS.cUa is expressed under the control of Scer\GAL4GMR.PF at 18[o]C. 95% of pupae eclose (compared to 69%). The reduction in eye size is also partially suppressed.
Larval wing disc cells expressing BacA\p35Scer\UAS.cHa and WScer\UAS.cUa under the control of Scer\GAL4ptc-559.1 display invading cells exclusively in basal planes of the tissue; these cells have cleanly detached and migrate several cell diameters away from the posterior edge of the ptc domain. They display a robust, rounded morphology indicative of healthy cells.
Knockdown of Nc, through expression of NcNIG.8091R completely suppresses the invasive cell phenotype found upon co-expression of BacA\p35Scer\UAS.cHa-WScer\UAS.cUa under the control of Scer\GAL4ptc-559.1.