FlyBase Allele Report: Dmel\shi[ts1.IVS.p10.20xUAS]

General Information

Symbol

Dmel\shi^{ts1.IVS.p10.20xUAS}

Species

D. melanogaster

Name

FlyBase ID

FBal0270222

Feature type

allele

Associated gene

Dmel\shi

Associated Insertion(s)

Carried in Construct

P{20XUAS-TTS-shi^ts1-p10}

PBac{20XUAS-IVS-shi^ts1-p10}

PBac{20XUAS-TTS-shi^ts1-p10}

Also Known As

UAS-shi^ts1, pJFRC100-20XUAS-TTS-Shibire-ts1-p10, UAS-shi^ts, UAS-Shibire^TS1, UAS-shibire^ts, UAS-Shi, UAS-20xShi.ts1, pJFRC100, 20XUAS-TTS-Shibire^ts1-p10, UAS-Shibirets

Key Links

Transgenic product class

dominant_negative_variant

(Pfeiffer et al., 2012)

missense_variant

(Pfeiffer et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Pfeiffer et al., 2012)

missense_variant

(Pfeiffer et al., 2012)

Mutagen

in vitro construct

(Pfeiffer et al., 2012)

Progenitor genotype

Carried in construct

P{20XUAS-TTS-shi^ts1-p10}

PBac{20XUAS-IVS-shi^ts1-p10}

PBac{20XUAS-TTS-shi^ts1-p10}

Cytology

Description

20 copies of the UAS site are fused upstream of the shi¹ coding region (this encodes a temperature-sensitive dominant negative form of shi). A small intron derived from the Mhc gene has been inserted between the promoter and coding sequences. The 3' UTR (666bp) from the Autographa californica nucleopolyhedrovirus (AcNPV) p10 gene is present downstream of the coding sequence.

(Pfeiffer et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

shi

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates epilepsy

modeled by eas²

(Kroll et al., 2015)

modeled by para^bss1

(Kroll et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior, with Scer\GAL4^0273-G4

(Lin et al., 2014)

abnormal behavior, with Scer\GAL4^GMR48B04

(Lin et al., 2014)

abnormal behavior, with Scer\GAL4^GMR48B04, Scer\GAL80⁰¹⁰⁴

(Lin et al., 2014)

abnormal behavior, with Scer\GAL4^GMR48B04, Scer\GAL80^R58E02

(Lin et al., 2014)

abnormal learning, with Scer\GAL4^Tdc2.PC

(Lin et al., 2014)

abnormal learning | temperature conditional, with Scer\GAL4^0273-G4

(Lin et al., 2014)

abnormal learning | temperature conditional, with Scer\GAL4^GMR58E02

(Lin et al., 2014)

abnormal memory | adult stage | :heat sensitive | chemical conditional, with Scer\GAL4^VT006554

(Shyu et al., 2017)

abnormal memory | adult stage | :heat sensitive | chemical conditional, with Scer\GAL4^VT008167

(Shyu et al., 2017)

abnormal memory | adult stage | :heat sensitive | conditional, with Hsap\RELA^AD.R12C11, Scer\GAL4^DBD.R14C08

(Aso et al., 2014)

abnormal memory | adult stage | :heat sensitive | conditional, with Hsap\RELA^AD.R14C08, Scer\GAL4^DBD.R15B01

(Aso et al., 2014)

abnormal memory | adult stage | :heat sensitive | conditional, with Hsap\RELA^AD.R15B01, Scer\GAL4^DBD.R27G01

(Aso et al., 2014)

abnormal memory | adult stage | :heat sensitive | conditional, with Hsap\RELA^AD.R20G03, Scer\GAL4^DBD.R19F09

(Aso et al., 2014)

abnormal memory | adult stage | :heat sensitive | conditional, with Hsap\RELA^AD.R24H08, Scer\GAL4^DBD.R53F03

(Aso et al., 2014)

abnormal memory | adult stage | :heat sensitive | conditional, with Hsap\RELA^AD.R25D01, Scer\GAL4^DBD.R19F09

(Aso et al., 2014)

abnormal memory | adult stage | :heat sensitive | conditional, with Hsap\RELA^AD.R30E08, Scer\GAL4^DBD.R53C10

(Aso et al., 2014)

abnormal memory | adult stage | :heat sensitive | conditional, with Hsap\RELA^AD.R40B08, Scer\GAL4^DBD.R23C06

(Aso et al., 2014)

abnormal memory | adult stage | :heat sensitive | conditional, with Hsap\RELA^AD.R53C03, Scer\GAL4^DBD.R24E12

(Aso et al., 2014)

abnormal memory | adult stage | :heat sensitive | conditional, with Hsap\RELA^AD.R64F07, Scer\GAL4^DBD.R57C10

(Aso et al., 2014)

abnormal memory | adult stage | :heat sensitive | conditional, with Hsap\RELA^AD.R65B09, Scer\GAL4^DBD.R11F03

(Aso et al., 2014)

abnormal memory | adult stage | :heat sensitive | conditional, with Hsap\RELA^AD.R65B09, Scer\GAL4^DBD.R51D04

(Aso et al., 2014)

abnormal memory | adult stage | :heat sensitive | conditional, with Hsap\RELA^AD.R70B10, Scer\GAL4^DBD.R19F09

(Aso et al., 2014)

abnormal memory | adult stage | :heat sensitive | conditional, with Hsap\RELA^AD.R71D08, Scer\GAL4^DBD.R11F03

(Aso et al., 2014)

abnormal memory | adult stage | :heat sensitive | conditional, with Hsap\RELA^AD.R73H08, Scer\GAL4^DBD.R40B08

(Aso et al., 2014)

abnormal memory | adult stage | :heat sensitive | conditional, with Hsap\RELA^AD.R93D10, Scer\GAL4^DBD.R13F04

(Aso et al., 2014)

abnormal memory | adult stage | :heat sensitive | nutrition conditional, with Hsap\RELA^AD.12xCRE, Scer\GAL4^DBD.Mef2.247

(Yamazaki et al., 2018)

abnormal memory | adult stage | heat sensitive, with Hsap\RELA^AD.R13F02, Scer\GAL4^DBD.R21B06

(Vogt et al., 2016)

abnormal memory | adult stage | heat sensitive, with Hsap\RELA^AD.R13F02, Scer\GAL4^DBD.R45H04

(Vogt et al., 2016)

abnormal memory | adult stage | heat sensitive, with Hsap\RELA^AD.R13F02, Scer\GAL4^DBD.R52H09

(Vogt et al., 2016)

abnormal memory | adult stage | heat sensitive, with Hsap\RELA^AD.R19B03, Scer\GAL4^DBD.R26E07

(Vogt et al., 2016)

abnormal memory | adult stage | heat sensitive, with Hsap\RELA^AD.R19B03, Scer\GAL4^DBD.R39A11

(Vogt et al., 2016)

abnormal memory | adult stage | heat sensitive, with Hsap\RELA^AD.R25D01, Scer\GAL4^DBD.R19F09

(Berry et al., 2018)

abnormal memory | adult stage | heat sensitive, with Hsap\RELA^AD.R26E07, Scer\GAL4^DBD.R39A11

(Vogt et al., 2016)

abnormal memory | adult stage | heat sensitive, with Hsap\RELA^AD.R28F07, Scer\GAL4^DBD.R10E05

(Vogt et al., 2016)

abnormal memory | adult stage | heat sensitive, with Hsap\RELA^AD.R30E11, Scer\GAL4^DBD.R22B12

(Aso et al., 2014)

abnormal memory | adult stage | heat sensitive, with Hsap\RELA^AD.R40B08, Scer\GAL4^DBD.R23C06

(Shyu et al., 2017)

abnormal memory | adult stage | heat sensitive, with Hsap\RELA^AD.R52B07, Scer\GAL4^DBD.R52H01

(Aso et al., 2014)

abnormal memory | adult stage | heat sensitive, with Hsap\RELA^AD.R52G04, Scer\GAL4^DBD.R17C11

(Aso et al., 2014)

abnormal memory | adult stage | heat sensitive, with Hsap\RELA^AD.R52G04, Scer\GAL4^DBD.R49F03

(Vogt et al., 2016)

abnormal memory | adult stage | heat sensitive, with Hsap\RELA^AD.R52H01, Scer\GAL4^DBD.R52B07

(Aso et al., 2014)

abnormal memory | adult stage | heat sensitive, with Hsap\RELA^AD.R58E02, Scer\GAL4^DBD.R37E10

(Yamagata et al., 2015)

abnormal memory | adult stage | heat sensitive, with Hsap\RELA^AD.R73H08, Scer\GAL4^DBD.R20G04

(Aso et al., 2014)

abnormal memory | adult stage | heat sensitive, with Scer\GAL4^5-HT1B.PY

(Shyu et al., 2017)

abnormal memory | adult stage | heat sensitive, with Scer\GAL4^c739

(Shyu et al., 2017)

abnormal memory | adult stage | heat sensitive, with Scer\GAL4^Ddc.PL

(Shyu et al., 2017)

abnormal memory | adult stage | heat sensitive, with Scer\GAL4^E1255

(Shyu et al., 2017)

abnormal memory | adult stage | heat sensitive, with Scer\GAL4^G0239

(Shyu et al., 2017)

abnormal memory | adult stage | heat sensitive, with Scer\GAL4^GMR15A04

(Yamagata et al., 2015)

abnormal memory | adult stage | heat sensitive, with Scer\GAL4^GMR16A06

(Shyu et al., 2017)

abnormal memory | adult stage | heat sensitive, with Scer\GAL4^GMR27G01

(Shyu et al., 2017)

abnormal memory | adult stage | heat sensitive, with Scer\GAL4^GMR48B04

(Shyu et al., 2017, Yamagata et al., 2015)

abnormal memory | adult stage | heat sensitive, with Scer\GAL4^GMR58E02

(Yamagata et al., 2015)

abnormal memory | adult stage | heat sensitive, with Scer\GAL4^Gr43a-GAL4

(Yamagata et al., 2015)

abnormal memory | adult stage | heat sensitive, with Scer\GAL4^VT019841

(Shyu et al., 2017)

abnormal memory | adult stage | heat sensitive, with Scer\GAL4^VT030604

(Shyu et al., 2017)

abnormal memory | adult stage | heat sensitive, with Scer\GAL4^VT049246

(Shyu et al., 2017)

abnormal memory | adult stage | heat sensitive, with Scer\GAL4^VT057242

(Shyu et al., 2017)

abnormal memory | adult stage | heat sensitive, with Scer\GAL4^VT057244

(Shyu et al., 2017)

abnormal phototaxis, with Ecol\lexA^DBD.C1a.ort, Hsim\VP16^{AD-VGlut1-OK371}

(Karuppudurai et al., 2014)

abnormal phototaxis, with Scer\GAL4^GMR21C07

(Pfeiffer et al., 2012)

abnormal sleep | adult stage | heat sensitive, with Hsap\RELA^AD.R19B03, Scer\GAL4^DBD.R39A11

(Sitaraman et al., 2015)

abnormal sleep | adult stage | heat sensitive, with Hsap\RELA^AD.R25D01, Scer\GAL4^DBD.R19F09

(Sitaraman et al., 2015)

paralytic | adult stage | heat sensitive, with Scer\GAL4^GMR57C10

(Pfeiffer et al., 2012)

Phenotype Manifest In

Detailed Description

Statement

Reference

Blocking synaptic transmission in Tm5c neurons by expressing Ctet\TeTxLC^TNT.Scer\UAS under the control of Scer\GAL4^{C1a.DBD.ort.T:Zzzz\ZipRREEL} and Hsim\VP16^{AD.VGlut.OK371.T:Zzzz\ZipEERRL,T:SV40\nls2} at the non-permissive temperature significantly reduces the phototactic preference for ultraviolet light over green light seen in wild type and control flies.

(Karuppudurai et al., 2014)

Expression of shi^{ts1.IVS.p10.20xScer\UAS} under the control of Scer\GAL4^GMR48B04 converts the behavior of naive thirsty flies from water approach to water avoidance. This behavioral reversal is not evident at the permissive temperature.

Expression of shi^{ts1.IVS.p10.20xScer\UAS} under the control of Scer\GAL4^GMR48B04 has no effect on water avoidance in sated flies, suggesting that these flies perceive water normally and that output from R48B04 neurons is only required for water approach in thirsty flies.

Addition of Scer\GAL80^GMR58E02 to flies expressing shi^{ts1.IVS.p10.20xScer\UAS} under the control of Scer\GAL4^GMR48B04 suppresses the behavioral defect of naive thirsty flies (back to water approach from water avoidance).

Addition of Scer\GAL80⁰¹⁰⁴ to flies expressing shi^{ts1.IVS.p10.20xScer\UAS} under the control of Scer\GAL4^GMR48B04 abolishes naive water-seeking behavior in thirsty flies.

(Lin et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^DBD.R19F09, shi^{ts1.IVS.p10.20xUAS}, Hsap\RELA^AD.R25D01 is an enhancer | heat sensitive of abnormal sleep | adult stage | heat sensitive phenotype of Ecol\lexA^GMR14H06, TrpA1^{13xlexAop.JFRC26}

(Sitaraman et al., 2015)

NOT Enhancer of

Statement

Reference

Scer\GAL4^DBD.R19F09, shi^{ts1.IVS.p10.20xUAS}, Hsap\RELA^AD.R25D01 is a non-enhancer of abnormal sleep | adult stage | heat sensitive phenotype of Ecol\lexA^GMR35B12, TrpA1^{13xlexAop.JFRC26}

(Sitaraman et al., 2015)

Suppressor of

Statement

Reference

shi^{ts1.IVS.p10.20xUAS}/Scer\GAL4^shot-OK307 is a suppressor of bang sensitive phenotype of para^bss1

(Kroll et al., 2015)

Scer\GAL4^GMR57C10/shi^{ts1.IVS.p10.20xUAS} is a suppressor of bang sensitive phenotype of eas²

(Kroll et al., 2015)

Scer\GAL4^ChAT.PU/shi^{ts1.IVS.p10.20xUAS} is a suppressor of bang sensitive phenotype of eas²

(Kroll et al., 2015)

shi^{ts1.IVS.p10.20xUAS}/Scer\GAL4^shot-OK307 is a suppressor of bang sensitive phenotype of eas²

(Kroll et al., 2015)

Scer\GAL4^GMR57C10/shi^{ts1.IVS.p10.20xUAS} is a suppressor of bang sensitive phenotype of para^bss1

(Kroll et al., 2015)

Scer\GAL4^elav-C155/shi^{ts1.IVS.p10.20xUAS} is a suppressor of bang sensitive phenotype of para^bss1

(Kroll et al., 2015)

Scer\GAL4^ChAT.PU/shi^{ts1.IVS.p10.20xUAS} is a suppressor of bang sensitive phenotype of para^bss1

(Kroll et al., 2015)

Scer\GAL4^RapGAP1-OK6/shi^{ts1.IVS.p10.20xUAS} is a suppressor of bang sensitive phenotype of para^bss1

(Kroll et al., 2015)

Scer\GAL4^ChAT.PU/shi^{ts1.IVS.p10.20xUAS} is a suppressor of abnormal neurophysiology phenotype of para^bss1

(Kroll et al., 2015)

Scer\GAL4^GMR57C10/shi^{ts1.IVS.p10.20xUAS} is a suppressor of abnormal neurophysiology phenotype of para^bss1

(Kroll et al., 2015)

Scer\GAL4^DBD.R15B01, Hsap\RELA^AD.R14C08, shi^{ts1.IVS.p10.20xUAS} is a suppressor | heat sensitive of abnormal sleep | adult stage | heat sensitive phenotype of Ecol\lexA^GMR35B12, TrpA1^{13xlexAop.JFRC26}

(Sitaraman et al., 2015)

NOT Suppressor of

Statement

Reference

Scer\GAL4^RapGAP1-OK6/shi^{ts1.IVS.p10.20xUAS} is a non-suppressor of abnormal neurophysiology phenotype of para^bss1

(Kroll et al., 2015)

Scer\GAL4^DBD.R19F09, shi^{ts1.IVS.p10.20xUAS}, Hsap\RELA^AD.R25D01 is a non-suppressor of abnormal sleep | adult stage | heat sensitive phenotype of Ecol\lexA^GMR35B12, TrpA1^{13xlexAop.JFRC26}

(Sitaraman et al., 2015)

Other

Statement

Reference

Scer\GAL4^shot-OK307, eas², shi^{ts1.IVS.p10.20xUAS} has abnormal neurophysiology phenotype

(Kroll et al., 2015)

Scer\GAL4^shot-OK307, para^bss1, shi^{ts1.IVS.p10.20xUAS} has abnormal neurophysiology phenotype

(Kroll et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of shi^{ts1.IVS.p10.20xScer\UAS} under the control of Scer\GAL4^GMR57C10 partially suppresses the bang sensitivity of hemizygous eas² flies when incubated for 3 min at 28[o]C, but not at 23[o]C.

shi^{ts1.IVS.p10.20xScer\UAS} under the control of Scer\GAL4^RapGAP1-OK6 does not rescue the bang sensitivity of hemizygous eas² flies temperature shifted to 28[o]C.

shi^{ts1.IVS.p10.20xScer\UAS} under the control of Scer\GAL4^ChAT.PU fully rescues, or under the control of Scer\GAL4^shot-OK307 partially rescues the bang sensitivity of hemizygous eas² flies temperature shifted to 28[o]C.

eas² mutants expressing shi^{ts1.IVS.p10.20xScer\UAS} under the control of Scer\GAL4^shot-OK307 display synaptic transmission defects and a higher seizure threshold when heated, as compared with controls.

Expression of shi^{ts1.IVS.p10.20xScer\UAS} under the control of Scer\GAL4^GMR57C10 partially suppresses the bang sensitivity of hemizygous para^bss1 flies when incubated for 3 min at 28[o]C, but not at 23[o]C; and increases the seizure threshold of para^bss1/+ flies at 23[o]C.

Expression of shi^{ts1.IVS.p10.20xScer\UAS} under the control of Scer\GAL4^GMR57C10 or Scer\GAL4^elav-C155 partially suppresses the bang sensitivity of para^bss1/+ flies at 23[o]C or 30[o]C, but not at 18[o]C.

shi^{ts1.IVS.p10.20xScer\UAS} under the control of Scer\GAL4^ChAT.PU partially suppresses the bang sensitivity, and increases the seizure threshold of para^bss1/+ flies at 23[o]C, and partially rescues the bang sensitivity of hemizygous para^bss1 flies temperature shifted to 28[o]C.

shi^{ts1.IVS.p10.20xScer\UAS} under the control of Scer\GAL4^RapGAP1-OK6 partially rescues the bang sensitivity, but does not significantly change the seizure threshold of para^bss1/+ flies at 23[o]C; and does not rescue the bang sensitivity of hemizygous para^bss1 flies temperature shifted to 28[o]C.

shi^{ts1.IVS.p10.20xScer\UAS} under the control of Scer\GAL4^shot-OK307 does not rescue the bang sensitivity, and does not significantly change the seizure threshold of para^bss1/+ flies at 23[o]C; but partially rescues the bang sensitivity of hemizygous para^bss1 flies temperature shifted to 28[o]C.

para^bss1 mutants expressing shi^{ts1.IVS.p10.20xScer\UAS} under the control of Scer\GAL4^shot-OK307 display synaptic transmission defects and a higher seizure threshold when heated, as compared with controls.

(Kroll et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

66599

y¹ w^*; PBac{20XUAS-IVS-shi^ts1-p10}VK00005

66600

y¹ w^*; PBac{20XUAS-TTS-shi^ts1-p10}VK00005

Notes on Origin

Discoverer

Comments

The P{20XUAS-IVS-shi^ts1-p10} construct contains a single copy of shi^{ts1.IVS.p10.20xScer\UAS}. The PBac{20XUAS-TTS-shi^ts1-p10} construct contains a tandem dimer of shi^{ts1.IVS.p10.20xScer\UAS}.

(Pfeiffer et al., 2012)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

shi^{ts1.IVS.p10.20xScer\UAS}

shi^{ts1.IVS.p10.20xUAS}

Name Synonyms

Secondary FlyBase IDs

References (61)

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