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FB2026_01
,
released March 12, 2026
pUf68, pUbsf, DPUF68, PUF60, poly U binding factor 68kD
RNA recognition motif protein - functions in both constitutive and alternative splicing - required during oogenesis - a single stranded nucleic acid binding protein - functions in Myc transcriptional repression and cell growth control, via interaction with the transcription factor Haywire.
Please see the JBrowse view of Dmel\hfp for information on other features
To submit a correction to a gene model please use the Contact FlyBase form
AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.45
2.5 (unknown)
Interacts with enc. However, given the cytoplasmic localization of enc, the relevance of such interaction is unclear.
The third RNA recognition motif, called PUMP domain in PubMed:10606266, is atypical and may rather mediate protein-protein interactions.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\hfp using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: 0-2 hr AEL
Comment: reference states 0-8 hr AEL
Developmental northern blot analysis indicates that the predominant 2.5 kb hfp transcript is highly enriched in early embryos and the ovary. Phenotypic information indicates that this gene is expressed at other stages as well.
hfp protein is detected in the nuclei of most cells in the wing disc, with higher staining in the ZNC, a zone of cells arrested in G1 or G2, along the dorsal/ventral compartment boundary in the wing disc.
Protein is detected in all nuclei of germline and somatic cells in the ovary when specific monoclonal antibodies are used for detection. When polyclonal sera is used cytoplasmic aggregates are detected, however, the significance of this result is unknown. Reduced amounts of protein are detected in the germline of viable hfp mutants suggesting that expression from a germline promoter is affected in these mutants.
JBrowse - Visual display of RNA-Seq signals
View Dmel\hfp in JBrowse
3-0.5
3-2.2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
monoclonal
pUf68 mutant larvae are produced at a normal frequency, however they undergo an extended larval stage and are significantly larger than wild type. Imaginal discs from hypomorphic pUf68 mutant larvae have increased S phases. Despite completing metamorphosis, pUf68 pupae die in their pupal case. These pupae are large, with malformed legs and consistent duplication of scutellar bristles.
Identification: as a protein that shows a specific interaction with enc protein in a yeast two-hybrid assay.
Area matching Drosophila EST AA568011.
Area matching Drosophila EST AA952055. This EST forms a 1205bp contgi with AA202358, AA202625 and AA951416. Sequence similarity to Huamn SIAH binding protein gene.
Source for merge of: pUf68 anon-WO0118547.408
scaRNA:pUf68-a is encoded in an intron of pUf68.
Source for merge of pUf68 anon-WO0118547.408 was sequence comparison ( date:051113 ).
Source for identity of: pUbsf Fir
Source for identity of: hfp pUf68
Renamed from 'pUf68' to 'hfp' to reflect preferred usage in the Drosophila literature.
