FlyBase Gene Report: Dmel\gw

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Gene: Dmel\gw

General Information

Symbol

Dmel\gw

Species

D. melanogaster

Name

gawky

Annotation Symbol

CG31992

Feature Type

protein_coding_gene

FlyBase ID

FBgn0051992

Gene Model Status

Stock Availability

10 publicly available

Gene Summary

Required for gene silencing mediated by micro-RNAs (miRNAs). Silences both polyadenylated and deadenylated mRNAs. Required for miRNA-mediated translational repression and mRNA decay. Not required for miRNA target recognition. Necessary to initiate but not to maintain silencing. Promotes mRNA deadenylation through the recruitment of the CCR4-NOT and PAN complexes and promotes decapping by the DCP1-DCP2 complex. Dissociates from silenced mRNAs after deadenylation. Required for completion of nuclear divisions during early embryonic development. (UniProt, Q8SY33)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

GW182, dGW182

Key Links

Genomic Location

Cytogenetic map

Sequence location

4:649,041..660,608 [-]

Recombination map

4-0

RefSeq locus

NC_004353 REGION:649041..660608

Sequence

Get Decorated FASTA

Genomic Maps

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (15 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with UniProtKB:A1Z7K9

(Braun et al., 2011)

inferred from physical interaction with UniProtKB:A8DY81

(Braun et al., 2011)

inferred from physical interaction with UniProtKB:E1JHY7

(Braun et al., 2011)

inferred from physical interaction with UniProtKB:Q7K126

(Braun et al., 2011)

inferred from physical interaction with UniProtKB:Q8IMX1

(Braun et al., 2011)

inferred from physical interaction with UniProtKB:Q94547

(Braun et al., 2011)

inferred from physical interaction with UniProtKB:Q95RR8

(Braun et al., 2011)

inferred from physical interaction with UniProtKB:Q32KD4

(Chekulaeva et al., 2010, Eulalio et al., 2009, Eulalio et al., 2008)

inferred from physical interaction with UniProtKB:P21187

(Zekri et al., 2009)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables nucleic acid binding

inferred from electronic annotation with InterPro:IPR035979

(InterPro Project Members, 2004-)

Biological Process (10 terms)

Terms Based on Experimental Evidence (8 terms)

CV Term

Evidence

References

involved_in 3'-UTR-mediated mRNA destabilization

inferred from direct assay

(Zhu et al., 2018)

involved_in embryonic development via the syncytial blastoderm

inferred from direct assay

(Schneider et al., 2006)

involved_in locomotor rhythm

inferred from mutant phenotype

(Zhang and Emery, 2013)

involved_in miRNA-mediated post-transcriptional gene silencing

inferred from mutant phenotype

(Chekulaeva et al., 2010, Chekulaeva et al., 2009, Eulalio et al., 2009, Eulalio et al., 2009, Zekri et al., 2009, Eulalio et al., 2008)

inferred from mutant phenotype

(Behm-Ansmant et al., 2006, Rehwinkel et al., 2005)

involved_in mRNA catabolic process

inferred from mutant phenotype

(Behm-Ansmant et al., 2006)

involved_in negative regulation of gene expression

inferred from direct assay

(Sienski et al., 2015)

involved_in regulation of protein localization

inferred from mutant phenotype

(Eulalio et al., 2009)

NOT involved_in nuclear-transcribed mRNA catabolic process, nonsense-mediated decay

inferred from mutant phenotype

(Rehwinkel et al., 2005)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in miRNA-mediated gene silencing by inhibition of translation

inferred from electronic annotation with InterPro:IPR033503

(InterPro Project Members, 2004-)

involved_in miRNA-mediated post-transcriptional gene silencing

inferred from biological aspect of ancestor with PANTHER:PTN000989337

(GO Reference Genome Project, 2011-)

involved_in positive regulation of nuclear-transcribed mRNA poly(A) tail shortening

inferred from biological aspect of ancestor with PANTHER:PTN000989337

(GO Reference Genome Project, 2011-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Sienski et al., 2015)

is_active_in P-body

inferred from direct assay

(Schneider et al., 2006)

located_in P-body

inferred from direct assay

(Eulalio et al., 2007)

part_of RISC complex

inferred from physical interaction with FLYBASE:AGO1; FB:FBgn0262739

(Miyoshi et al., 2009)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in P-body

inferred from biological aspect of ancestor with PANTHER:PTN000989337

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

miRNA-INDUCED SILENCING COMPLEX

Protein Family (UniProt)

Belongs to the GW182 family. (Q8SY33)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

miRNA-INDUCED SILENCING COMPLEX -: The miRNA RNA-induced silencing complex (miRISC) mediates post-transcriptional repression of gene expression by incorporating mature miRNA and down-regulating the expression of the target mRNA via repression of translation or mRNA degradation. (Adapted from FBrf0247552 and FBrf0194513.)

Protein Function (UniProtKB)

Required for gene silencing mediated by micro-RNAs (miRNAs). Silences both polyadenylated and deadenylated mRNAs. Required for miRNA-mediated translational repression and mRNA decay. Not required for miRNA target recognition. Necessary to initiate but not to maintain silencing. Promotes mRNA deadenylation through the recruitment of the CCR4-NOT and PAN complexes and promotes decapping by the DCP1-DCP2 complex. Dissociates from silenced mRNAs after deadenylation. Required for completion of nuclear divisions during early embryonic development.

(UniProt, Q8SY33)

Summary (Interactive Fly)

RNA-binding protein - glycine-tryptophan (GW) repeat protein required for P-body integrity - promotes mRNA deadenylation, decapping and degradation

Interactive Fly

Gene Model and Products

Number of Transcripts

5

Number of Unique Polypeptides

2

Please see the JBrowse view of Dmel\gw for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q8SY33)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

2WBR

Comments on Gene Model

Gene model reviewed during 5.43

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.46

Gene model reviewed during 5.47

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Gene model reviewed during 6.32

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0089100

5036

1384

FBtr0089096

4792

1384

FBtr0089097

4675

1384

FBtr0089101

4718

1384

FBtr0310543

5716

1382

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

FBpp0088169

143.0

1384

6.72

FBpp0088165

143.0

1384

6.72

FBpp0088166

143.0

1384

6.72

FBpp0088170

143.0

1384

6.72

FBpp0302680

142.7

1382

6.69

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1384 aa isoforms: gw-PA, gw-PB, gw-PE, gw-PF

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Component of the miRNA-directed RNA-induced silencing complex (miRISC), composed of at least AGO1 and gw, which bind mature miRNAs and targets the selective destruction of homologous RNAs (PubMed:16815998, PubMed:18345015, PubMed:19451544). Interacts (via N-terminal region) with AGO1 (via Piwi domain); the interaction is essential for localization of AGO1 in P-bodies and for miRNA-mediated silencing (PubMed:16815998, PubMed:18345015, PubMed:19451544). Interacts with pAbp/PABPC1; this interaction interferes with the binding of pAbp to eIF4G and is required for miRNA-mediated silencing (PubMed:19797087). Interacts with CCR4-NOT complex members Not1, Rga/NOT2, twin/CCR4, Pop2 and NOT3/5 and with PAN complex members CG8232/PAN2 and CG11486/PAN3 (PubMed:21981923).

(UniProt, Q8SY33)

Domain

The UBA domain is not required for correct subcellular location, gene silencing or interaction with pAbp.

The RRM domain lacks RNA-binding properties and does not bind RNA in vitro. It is not required for P-body localization or for interaction with AGO1 or miRNAs but is required for silencing. May play a role in protein-protein interactions.

(UniProt, Q8SY33)

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

2WBR

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Q8SY33

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\gw using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.13

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

spermatogenesis

male germline cyst

(Insco et al., 2012)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Sienski et al., 2015)

is_active_in P-body

inferred from direct assay

(Schneider et al., 2006)

located_in P-body

inferred from direct assay

(Eulalio et al., 2007)

part_of RISC complex

inferred from physical interaction with FLYBASE:AGO1; FB:FBgn0262739

(Miyoshi et al., 2009)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\gw in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\gw

View exonic expression by tissue for Dmel\gw

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

43808/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0051992

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0051992

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0051992

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG31992

Flygut - An atlas of the Drosophila adult midgut

FBgn0051992

Images

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 18 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 15 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of gw

Transgenic constructs containing regulatory region of gw

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

(Podemski et al., 2004)

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal (with Df(4)ED6380)

gw^{CR70482-TG4.0}

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

gw^GD10428

partially lethal - majority die, with Scer\GAL4^pnr-MD237

gw^GD10428

gw^39C-33

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

gw^GD10428

viable, with Scer\GAL4^tin.CΔ4

gw^GD10428

Other Phenotypes

Allele

abnormal locomotor rhythm, with Dcr-2^UAS.cDa, Scer\GAL4^tim.PE

abnormal locomotor rhythm, with Dcr-2^UAS.cDa, Scer\GAL4^tim.PE, Scer\GAL80^Pdf.PS

gw^KK101472

some die during pupal stage, with Scer\GAL4^pnr-MD237

gw^GD10428

visible, with Scer\GAL4^pnr-MD237

gw^GD10428

Phenotype manifest in

Allele

mesonotum, with Scer\GAL4^pnr-MD237

gw^GD10428

trichogen cell, with Scer\GAL4^pnr-MD237

gw^GD10428

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (3)

8 of 14

Yes

Yes

1

5 of 14

No

Yes

1

5 of 14

No

Yes

1

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (3)

7 of 14

Yes

Yes

6 of 14

No

Yes

6 of 14

No

Yes

Mus musculus (laboratory mouse) (3)

7 of 14

Yes

Yes

5 of 14

No

Yes

5 of 14

No

Yes

Xenopus tropicalis (Western clawed frog) (3)

5 of 13

Yes

Yes

4 of 13

No

Yes

4 of 13

No

Yes

Danio rerio (Zebrafish) (6)

7 of 14

Yes

Yes

6 of 14

No

Yes

6 of 14

No

Yes

4 of 14

No

Yes

4 of 14

No

Yes

3 of 14

No

Yes

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (3)

Agam\AgaP_AGAP007802

10 of 12

Yes

Yes

Agam\AgaP_AGAP007803

10 of 12

Yes

Yes

Agam\AgaP_AGAP007808

8 of 12

No

Yes

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:gw. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

TNRC6A; trinucleotide repeat containing adaptor 6A

model of familial adult myoclonic epilepsy 6

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TNRC6C; trinucleotide repeat containing adaptor 6C

8 of 14

TNRC6A; trinucleotide repeat containing adaptor 6A

5 of 14

EPILEPSY, FAMILIAL ADULT MYOCLONIC, 6; FAME6

familial adult myoclonic epilepsy 6

TNRC6B; trinucleotide repeat containing adaptor 6B

5 of 14

GLOBAL DEVELOPMENTAL DELAY WITH SPEECH AND BEHAVIORAL ABNORMALITIES; GDSBA

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

anti tag coimmunoprecipitation, anti tag western blot

(Kaul et al., 2024)

anti tag coimmunoprecipitation, anti tag western blot, coimmunoprecipitation, western blot

(Jia et al., 2021)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, western blot

(Huntzinger et al., 2013, Braun et al., 2011)

anti tag coimmunoprecipitation, anti tag western blot

(Braun et al., 2011)

anti tag coimmunoprecipitation, anti tag western blot

(Huntzinger et al., 2013, Braun et al., 2011)

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot

(Barišić-Jäger et al., 2013, Jäger and Dorner, 2010)

experimental knowledge based

(Guruharsha et al., 2011)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, anti tag western blot

(Huntzinger et al., 2013, Braun et al., 2011)

pull down, western blot

(Antic et al., 2015)

anti bait coimmunoprecipitation, western blot

(Xiong et al., 2015)

experimental knowledge based

(Guruharsha et al., 2011)

anti bait coimmunoprecipitation, western blot

(Li et al., 2012)

experimental knowledge based

(Guruharsha et al., 2011)

pull down, anti tag western blot, anti tag coimmunoprecipitation, molecular weight estimation by staining

(Huntzinger et al., 2013, Fukaya and Tomari, 2011, Huntzinger et al., 2010, Zekri et al., 2009)

RNA-protein

Physical Interaction

Assay

References

anti bait coimmunoprecipitation, primer specific pcr

(Gehrke et al., 2015)

anti tag coimmunoprecipitation, northern blot, anti bait coimmunoprecipitation

(Eulalio et al., 2009, Eulalio et al., 2009, Miyoshi et al., 2009, Zekri et al., 2009)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Zhang and Emery, 2013)

suppressible

(Zhang and Emery, 2013)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Gehrke et al., 2015)

External Data

Subunit Structure (UniProtKB)

Component of the miRNA-directed RNA-induced silencing complex (miRISC), composed of at least AGO1 and gw, which bind mature miRNAs and targets the selective destruction of homologous RNAs (PubMed:16815998, PubMed:18345015, PubMed:19451544). Interacts (via N-terminal region) with AGO1 (via Piwi domain); the interaction is essential for localization of AGO1 in P-bodies and for miRNA-mediated silencing (PubMed:16815998, PubMed:18345015, PubMed:19451544). Interacts with pAbp/PABPC1; this interaction interferes with the binding of pAbp to eIF4G and is required for miRNA-mediated silencing (PubMed:19797087). Interacts with CCR4-NOT complex members Not1, Rga/NOT2, twin/CCR4, Pop2 and NOT3/5 and with PAN complex members CG8232/PAN2 and CG11486/PAN3 (PubMed:21981923).

(UniProt, Q8SY33 )

Linkouts

BioGRID - A database of protein and genetic interactions.

68639

DroID - A comprehensive database of gene and protein interactions.

FBgn0051992

MIST (protein-protein) - An integrated Molecular Interaction Database

43808

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

4

Recombination map

4-0

Cytogenetic map

Sequence location

4:649,041..660,608 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

102D2-102D3

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

4-0

(FlyBase, 2016)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (10)

Aberrations including deletions of this gene Aberrations including duplications of this gene

60863

y¹; Mi{MIC}gw^MI14022

22311

y¹; P{EPgy2}gw^EY05379

62024

y¹; P{SUPor-P}gw^KG08170b

81986

y¹ sc^* v¹ sev²¹; P{TKO.GS04175}attP40

34796

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00105}attP2/TM3, Sb¹

97747

y¹ w¹¹¹⁸; TI{DH.0}gw^{CR70482-DH.PT-GFSTF.0}/TI{GMR-HMS04515}Gat^eya

97184

y¹ w^*; TI{CRIMIC.TG4.0}gw^{CR70482-TG4.0}/In(4)ci^D, ci^D pan^ciD

118968

y¹ w¹¹¹⁸; TI{DH.0}gw^{CR70482-DH.PT-GFSTF.0}/TI{GMR-HMS04515}Gat^eya

v103581

P{KK101472}VIE-260B

v45772

w¹¹¹⁸; P{GD10428}v45772

Genomic Clones (20)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (181)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0051992

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0051992

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Matsuura-Suzuki et al., 2024, Su et al., 2024, Barišić-Jäger et al., 2013, Braun et al., 2011, Behm-Ansmant et al., 2006, Schneider et al., 2006)

monoclonal

(Na et al., 2016, Miyoshi et al., 2009)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

New stable cell line derived from S2-unspecified : S2 cell lines expressing circCG32369, circMCPH1, circeIF5B, circrl, circPde11, circCG17715, circzfh2, circTao, circcrol, circdrn, circMeltrin, circpxb, circfru, circdati, circstw, circSarm, circStim, circCG2991, circPvr, circHil, circmub, circsxc, circbnl, and circCG9743 were created. The author reports new stable cell lines S2-circMCPH1, S2-circCG32369, S2-Flag-Ddx56 , and S2-Flag-gw.
(Su et al., 2024)

Other Comments

gw-mediated mRNA decay involves the Pop2 :Not1 complex for deadenylation and the Dcp1 :Dcp2 complex for decapping.

(Behm-Ansmant et al., 2006)

gw silences expression of bound transcripts by two mechanisms: one involving inhibition of protein expression, and one involving a reduction of mRNA levels.

(Behm-Ansmant et al., 2006)

gw links the miRNA pathway to mRNA degradation by interacting with AGO1 and promoting decay of at least a subset of miRNA targets.

(Behm-Ansmant et al., 2006)

dsRNA made from templates generated with primers directed against this gene are used to infer a role for this gene in the miRNA pathway.

(Behm-Ansmant et al., 2006)

Loss of gw leads to breakdown of the cortical cytoskeleton and nuclear expansion.

(Schneider et al., 2006)

Mutant embryos have severe abnormalities, including disruptions in the precisely timed early embryonic nuclear divisions that normally occur prior to cellularization of the syncytial embryo.

(Simmonds et al., 2006)

dsRNA has been made from templates generated with primers directed against this gene.

(Rehwinkel et al., 2005)

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

Relationship to Other Genes

Source for database merge of

Source for merge of: CG9905 anon- EST:Posey205

(Bayraktaroglu, 2000.11.2)

Source for merge of: CG11484 CG9905

(FlyBase, 1996-)

Additional comments

FlyBase curator comment: Correspondence with author Jacquier revealed that 'Gawki' = 'gw' (CG31992).

(Jacquier et al., 2008)

Annotations CG11484, CG9905 merged as CG31992 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Source for merge of CG11484 CG9905 was a shared cDNA ( date:010720 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: gw CG31992

(Simmonds, 2006.3.2)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

CG11484

CG31992

(Kumar et al., 2016, Na et al., 2016, Hosono et al., 2015, Schertel et al., 2013, Ni et al., 2011.7.19, Jäger and Dorner, 2010, Müller et al., 2010, Keleman et al., 2009.8.5, Riddle et al., 2008, Johansson et al., 2007, Bjorklund et al., 2006, Schneider et al., 2006, Rehwinkel et al., 2005, Sousa-Neves et al., 2005, Lawniczak and Begun, 2004, Sun et al., 2004)

CG9905

(Boutros et al., 2004)

GQ182

(McCann et al., 2011)

GW

(Wilby and Weil, 2023, Schneider et al., 2006)

GW182

(Matsuura-Suzuki et al., 2024, Hamid and Akgül, 2022, Galagali and Kim, 2020, Jia et al., 2019, Cao et al., 2016, Sanfilippo et al., 2016, Sienski et al., 2015, Tataroglu and Emery, 2015, Xiong et al., 2015, Sudhakaran et al., 2014, Temme et al., 2014, Barckmann and Simonelig, 2013, Gomes et al., 2013, Hur et al., 2013, Lim and Allada, 2013, Nishihara et al., 2013, Smibert et al., 2013, Wu et al., 2013, Zekri et al., 2013, Chan and Kocerha, 2012, Collart and Panasenko, 2012, Insco et al., 2012, Li et al., 2012, Chekulaeva et al., 2010, Huntzinger et al., 2010, Iwasaki et al., 2010, Jäger and Dorner, 2010, Moretti et al., 2010, Borah et al., 2009, Chekulaeva et al., 2009, Eulalio et al., 2009, Eulalio et al., 2009, Eulalio et al., 2009, Iwasaki et al., 2009, Kawamata et al., 2009, Lauwers et al., 2009, Miyoshi et al., 2009, Zekri et al., 2009, Eulalio et al., 2008, Eulalio et al., 2007, Eulalio et al., 2007, Behm-Ansmant et al., 2006, Valencia-Sanchez et al., 2006, Rehwinkel et al., 2005)

Gw

(Temme et al., 2010)

Gw182

(Busto et al., 2017, Gehrke et al., 2015, Meister, 2013)

anon-EST:Posey205

dGW182

(Chekulaeva et al., 2011, Chekulaeva et al., 2009)

gw182

(Zhang and Emery, 2013)

Name Synonyms

Gawki

(Jacquier et al., 2008)

Gawky

(Burke, 2022, Sienski et al., 2015, Miyoshi et al., 2009)

gawky

(Goldsmith et al., 2022, Jia et al., 2022, Mugat et al., 2015, Temme et al., 2014, Handler et al., 2013, Temme et al., 2010, Schneider et al., 2006, Simmonds, 2006.3.2, Simmonds et al., 2006)

Secondary FlyBase IDs

FBgn0027730
FBgn0039917
FBgn0039918

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 76 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

43808

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q8SY33

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q8SY33

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q8SY33

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

43808/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0051992

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0051992

FlyMine - An integrated database for Drosophila genomics

FBgn0051992

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG31992

MARRVEL_MODEL - MARRVEL (model organism gene)

43808

PDB - An information portal to biological macromolecular structures

2WBR

Linkouts

BioGRID - A database of protein and genetic interactions.

68639

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0051992

DroID - A comprehensive database of gene and protein interactions.

FBgn0051992

DRSC - Results frm RNAi screens

FBgn0051992

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0051992

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0051992

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG31992

Flygut - An atlas of the Drosophila adult midgut

FBgn0051992

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0051992

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC006679

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

43808

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (151)