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General Information
Symbol
lexA::UnkAD
FlyBase ID
FBto0000158
Name
Description
Description

The lexA::UnkAD entry in FlyBase represents an artificial transcriptional activator ('driver') that includes the DNA-binding domain from the Escherichia coli lexA gene (ECOGENE:EG10533) and a transcriptional activation domain of unknown or unspecified origin. In Escherichia coli the lexA protein acts as a transcriptional repressor, binding specifically to the lexAop DNA sequence via its DNA-binding domain (reviewed in PMID:18726173). By fusing the lexA DNA-binding domain to a transcriptional activation domain, the resulting lexA::UnkAD protein is converted into a transcriptional activator that specifically binds lexAop. lexAop and lexA::UnkAD thus form a binary expression system that can be used to control the spatial and temporal expression of a gene of interest: a transgene or modified endogenous locus carrying the target gene of interest downstream of lexAop sequences is combined with a second transgene or modified endogenous locus encoding the lexA::UnkAD driver.

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Insertions ( 40 )
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Reported As
Symbol Synonym
lexA::UnkAD
Name Synonyms
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    References (57)