EC3, EG:BACR37P7.7 , l(1)EC3, l(1)1Ag
nuclear respiratory factor-1 transcription factor - regulates synaptic growth - regulates spatio-temporal coordination of cell cycle exit, fusion and differentiation of adult muscle precursors
Gene model reviewed during 5.41
Unconventional translation start (CUG) postulated; FBrf0059052.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Gene model reviewed during 5.46
Multiphase exon postulated: exon reading frame differs in alternative transcripts.
6.0, 5.2, 5.1, 4.3, 3.7 (northern blot)
733 (aa); 116 (kD observed)
Homodimer. Binds DNA as a dimer (By similarity).
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ewg using the Feature Mapper tool.
Comment: reference states 10-36 hr APF
GBrowse - Visual display of RNA-Seq signalsView Dmel\ewg in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
The enrichment of the 116kD ewg gene product is enriched in the head due to tissue-specific alternate and inefficient splicing, not by transcriptional regulation. The distinction is biologically relevant: an increased level of the 116kD ewg gene product outside the nervous system causes lethality.
Expression in the nervous system can rescue the embryonic lethality of ewg mutants but not the adult muscle defects. Expression from a heat shock promoter can restore IFM defects.
Mutations in ewg cause an early patterning defect and degeneration of the indirect flight muscles (IFM) at the onset of their differentiation. The developmental defects can be rescued by heat induced expression of ewg.
Protein encoded by the ewg open reading frame is sufficient to provide both the embryonic function and the requirement for the formation of indirect flight muscles.
Viable alleles cause wings to be held upright; wing posture phenotype shows incomplete penetrance. Dorso-ventral flight muscles often absent, especially when the mutation is heterozygous with a deficiency. Ultrastructure of tergal depressor of trochanter jump muscle normal. Flies hemizygous for lethal alleles die either just prior to hatching of the larva from the egg or immediately thereafter; mosaic analysis of certain lethal alleles suggests primary defect in developing muscles.