FlyBase Gene Report: Dmel\CTCF

General Information

Symbol

Dmel\CTCF

Species

D. melanogaster

Name

CTCF

Annotation Symbol

CG8591

Feature Type

protein_coding_gene

FlyBase ID

FBgn0035769

Gene Model Status

Current

Stock Availability

15 publicly available

Gene Summary

CTCF (CTCF) encodes a ubiquitous transcription factor that binds to insulators and domain boundaries. It mediates insulator function and blocks enhancers by binding to the product of Cp190. It contributes to long-range chromatin interaction, organizes chromatin domain boundaries and coordinates nuclear architecture. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

dCTCF, CCCTC-binding factor

Key Links

Genomic Location

Cytogenetic map

65F6-65F6

Sequence location

3L:7,353,578..7,356,713 [-]

Recombination map

3-20

RefSeq locus

NT_037436 REGION:7353578..7356713

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (14 terms)

Molecular Function (7 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

enables chromatin insulator sequence binding

inferred from direct assay

(Smith et al., 2009, Mohan et al., 2007, Moon et al., 2005)

enables DNA binding

inferred from direct assay

(Ogiyama et al., 2018)

enables protein binding

inferred from physical interaction with UniProtKB:Q9VCH5

(Pascual-Garcia et al., 2017)

enables sequence-specific DNA binding

inferred from direct assay

(Smith et al., 2009, Moon et al., 2005)

enables transcription cis-regulatory region binding

inferred from direct assay

(Magbanua et al., 2015)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables DNA-binding transcription factor activity

inferred from biological aspect of ancestor with PANTHER:PTN002811104

(GO Reference Genome Project, 2011-)

enables RNA polymerase II cis-regulatory region sequence-specific DNA binding

inferred from biological aspect of ancestor with PANTHER:PTN002811104

(GO Reference Genome Project, 2011-)

Biological Process (6 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

involved_in genomic imprinting

inferred from mutant phenotype

(Macdonald et al., 2010)

involved_in maintenance of rDNA

inferred from direct assay

(Guerrero and Maggert, 2011)

involved_in negative regulation of DNA-templated transcription

inferred from direct assay

(Smith et al., 2009, Moon et al., 2005)

involved_in response to ecdysone

inferred from direct assay

(Pascual-Garcia et al., 2017)

involved_in segment specification

inferred from mutant phenotype

(Mohan et al., 2007)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN002811104

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Moon et al., 2005)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN002811104

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

C2H2 ZINC FINGER TRANSCRIPTION FACTORS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

C2H2 ZINC FINGER TRANSCRIPTION FACTORS -: Zinc finger C2H2 transcription factors are sequence-specific DNA binding proteins that regulate transcription. They possess DNA-binding domains that are formed from repeated Cys₂His₂ zinc finger motifs. (Adapted from PMID:1835093, FBrf0220103 and FBrf0155739).

Summary (Interactive Fly)

CCCTC-binding factor is a zinc finger transcription factor that confers enhancer blocking in all known chromatin insulators

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CTCF for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VS55)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.45

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CTCF-RA

FBtr0076878

NM_139852

2892

818

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

CTCF-PA

FBpp0076588

93.1

818

6.14

Q9VS55

NP_648109

AAF50573

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CTCF using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CTCF_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.48

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 9 -- 10

central brain primordium

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 11 -- 12

central brain primordium

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Moon et al., 2005)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\CTCF in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CTCF

View exonic expression by tissue for Dmel\CTCF

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0035769

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38817/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0035769

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0035769

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0035769

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8591

Flygut - An atlas of the Drosophila adult midgut

FBgn0035769

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\CTCF

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 32 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 21 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CTCF

Transgenic constructs containing regulatory region of CTCF

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3L)0463

(Mohan et al., 2007)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Act5C.PI

CTCF^GL00266

lethal (with CTCF^attP.mCh)

CTCF^C2

lethal (with CTCF^C2)

CTCF^attP.mCh

lethal | recessive

CTCF³⁰

lethal - all die before end of P-stage

lethal - all die before end of P-stage (with Df(3L)0463)

CTCF^P30.6

lethal - all die before end of pupal stage | maternal effect

CTCF^KO

lethal - all die during embryonic stage, with Scer\GAL4^Act5C.PI

CTCF^UAS.EGFP

lethal - all die during embryonic stage, with Scer\GAL4^αTub84B.PL

CTCF^UAS.EGFP

lethal - all die during pharate adult stage (with CTCF^P35.2)

CTCF^y+1

lethal - all die during pharate adult stage (with CTCF^y+1)

CTCF^P35.2

partially lethal - majority die

partially lethal - majority die, with Scer\GAL4^pnr-MD237

CTCF^GD5052

partially lethal - majority die (with CTCF⁹)

CTCF^P35.2

partially lethal - majority die (with CTCF^attP.mCh)

CTCF^C1

partially lethal - majority die (with CTCF^C1)

partially lethal - majority die (with CTCF^C2)

CTCF^C1

partially lethal - majority die (with CTCF^GE24185)

CTCF^KO

partially lethal - majority die (with CTCF^KO)

CTCF^GE24185

partially lethal - majority die (with CTCF^P35.2)

CTCF⁹

partially lethal - majority live

partially lethal - majority live (with CTCF^attP.mCh)

CTCF^GE24185

partially lethal - majority live (with CTCF^C1)

CTCF^GE24185

partially lethal - majority live (with CTCF^C2)

CTCF^GE24185

partially lethal - majority live (with CTCF^GE24185)

partially lethal - majority live (with CTCF^KO)

CTCF^P30.6

partially lethal - majority live (with CTCF^P30.6)

CTCF^KO

partially lethal - majority live | maternal effect (with CTCF^GE24185)

CTCF^KO

partially lethal - majority live | maternal effect (with CTCF^KO)

CTCF^GE24185

viable

viable (with CTCF^EY15833)

CTCF^y+1

viable (with CTCF^GE24185)

CTCF^y+1

viable (with CTCF^y+1)

Sterility

Allele

female semi-fertile

CTCF^GE24185

female sterile (with CTCF^C1)

CTCF^GE24185

female sterile (with CTCF^GE24185)

CTCF^C1

fertile

sterile

sterile (with CTCF^attP.mCh)

sterile (with CTCF^attP)

CTCF^GE24185

sterile (with CTCF^C1)

sterile (with CTCF^C2)

CTCF^C1

sterile (with CTCF^GE24185)

Other Phenotypes

Allele

abnormal developmental rate

decreased size | adult stage | female

CTCF^GE24185

enhancer of variegation

flightless, with Scer\GAL4^Mef2.PR

CTCF^GD5052

homeotic

homeotic (with CTCF^EY15833)

CTCF^y+1

homeotic (with CTCF^GE24185)

CTCF^y+1

homeotic (with CTCF^y+1)

homeotic | adult stage

homeotic | adult stage (with CTCF^attP)

CTCF^C1

homeotic | adult stage (with CTCF^C1)

CTCF^attP

homeotic | adult stage (with CTCF^GE24185)

CTCF^KO

homeotic | adult stage (with CTCF^KO)

homeotic | adult stage (with CTCF^P30.6)

homeotic | adult stage (with CTCF^y+1)

CTCF^P30.6

homeotic | adult stage | maternal effect

CTCF^KO

homeotic | adult stage | maternal effect (with CTCF^GE24185)

CTCF^KO

homeotic | adult stage | maternal effect (with CTCF^KO)

CTCF^GE24185

homeotic | maternal effect

CTCF^GE24185

homeotic | pharate adult stage (with CTCF⁹)

CTCF^P35.2

homeotic | pharate adult stage (with CTCF^P35.2)

homeotic | pharate adult stage (with CTCF^y+1)

CTCF^P35.2

homeotic | P-stage

majority die during pharate adult stage (with CTCF⁹)

CTCF^P35.2

majority die during pharate adult stage (with CTCF^P35.2)

CTCF⁹

majority die during P-stage (with CTCF^GE24185)

CTCF^KO

majority die during P-stage (with CTCF^KO)

CTCF^GE24185

non-enhancer of variegation

non-suppressor of variegation

CTCF³⁰

short lived

CTCF^KO

short lived (with CTCF^KO)

short lived (with CTCF^P30.6)

CTCF^KO

short lived (with CTCF^y+1)

CTCF^KO

some die during embryonic stage, with Scer\GAL4^Act5C.PI

CTCF^UAS.EGFP

some die during embryonic stage, with Scer\GAL4^αTub84B.PL

CTCF^UAS.EGFP

some die during larval stage

CTCF^KO

some die during larval stage | maternal effect

CTCF^GE24185

some die during pharate adult stage

some die during pharate adult stage (with CTCF^KO)

CTCF^P30.6

some die during pharate adult stage (with CTCF^P30.6)

some die during pharate adult stage (with CTCF^y+1)

CTCF^P30.6

some die during pharate adult stage (with Df(3L)0463)

CTCF^P30.6

some die during pupal stage

CTCF^GE24185

some die during pupal stage, with Scer\GAL4^pnr-MD237

CTCF^GD5052

suppressor of variegation

CTCF³⁰

visible, with Scer\GAL4^Bx-MS1096

CTCF^{UAS.ORF.GW.Tag:HA}

visible | adult stage

visible | adult stage, with Scer\GAL4^en.PU

CTCF^GL00266

visible | adult stage, with Scer\GAL4^ey.PH

CTCF^GL00266

visible | adult stage (with CTCF^attP.mCh)

visible | adult stage (with CTCF^attP)

visible | adult stage (with CTCF^C1)

visible | adult stage (with CTCF^GE24185)

visible | homeotic

visible | homeotic (with Df(3L)0463)

visible | P-stage

visible | P-stage (with CTCF^attP.mCh)

CTCF^C2

visible | P-stage (with CTCF^C2)

CTCF^attP.mCh

Phenotype manifest in

Allele

abdomen | adult stage

abdomen | adult stage (with CTCF^GE24185)

CTCF^KO

abdomen | adult stage (with CTCF^KO)

abdomen | adult stage (with CTCF^P30.6)

abdomen | adult stage (with CTCF^y+1)

CTCF^P30.6

abdomen | adult stage | maternal effect

abdomen | adult stage | maternal effect (with CTCF^GE24185)

CTCF^KO

abdomen | adult stage | maternal effect (with CTCF^KO)

CTCF^GE24185

abdominal segment 4

CTCF^GE24185

abdominal segment 4 (with Df(3L)0463)

CTCF^GE24185

abdominal segment 5

abdominal segment 5 (with Df(3L)0463)

CTCF^P30.6

abdominal segment 6

abdominal segment 6 (with CTCF^EY15833)

CTCF^y+1

abdominal segment 6 (with CTCF^GE24185)

CTCF^y+1

abdominal segment 6 (with CTCF^y+1)

abdominal segment 6 | pharate adult stage (with CTCF⁹)

CTCF^P35.2

abdominal segment 6 | pharate adult stage (with CTCF^P35.2)

abdominal segment 6 | pharate adult stage (with CTCF^y+1)

CTCF^P35.2

abdominal segment 7

abdominal segment 7 (with CTCF^EY15833)

CTCF^y+1

abdominal segment 7 (with CTCF^GE24185)

CTCF^y+1

abdominal segment 7 (with CTCF^y+1)

abdominal segment 7 | pharate adult stage (with CTCF⁹)

CTCF^P35.2

abdominal segment 7 | pharate adult stage (with CTCF^P35.2)

abdominal segment 7 | pharate adult stage (with CTCF^y+1)

CTCF^P35.2

abdominal segment 8

abdominal segment 8 (with CTCF^EY15833)

CTCF^y+1

abdominal segment 8 (with CTCF^GE24185)

CTCF^y+1

abdominal segment 8 (with CTCF^y+1)

abdominal segment 8 | pharate adult stage (with CTCF⁹)

CTCF^P35.2

abdominal segment 8 | pharate adult stage (with CTCF^P35.2)

abdominal segment 8 | pharate adult stage (with CTCF^y+1)

CTCF^P35.2

abdominal segment | adult stage

abdominal segment | adult stage (with CTCF^GE24185)

CTCF^KO

abdominal segment | adult stage (with CTCF^KO)

abdominal segment | adult stage (with CTCF^P30.6)

abdominal segment | adult stage (with CTCF^y+1)

CTCF^P30.6

abdominal segment | adult stage | maternal effect

abdominal segment | adult stage | maternal effect (with CTCF^GE24185)

CTCF^KO

abdominal segment | adult stage | maternal effect (with CTCF^KO)

CTCF^GE24185

abdominal sternite

abdominal sternite 5

abdominal sternite 5 (with Df(3L)0463)

CTCF^P30.6

abdominal sternite 6

CTCF^GE24185

abdominal sternite 6 (with Df(3L)0463)

CTCF^GE24185

abdominal sternite 6 & abdominal sternite bristle

abdominal sternite 6 & abdominal sternite bristle (with Df(3L)0463)

CTCF^P30.6

abdominal sternite 6 | adult stage

abdominal sternite 7 (with Df(3L)0463)

CTCF^GE24185

abdominal sternite (with CTCF^EY15833)

CTCF^y+1

abdominal sternite (with CTCF^GE24185)

CTCF^y+1

abdominal sternite (with CTCF^y+1)

abdominal sternite | adult stage

abdominal sternite | adult stage (with CTCF^attP)

CTCF^C1

abdominal sternite | adult stage (with CTCF^C1)

CTCF^attP

abdominal sternite | pharate adult stage (with CTCF⁹)

CTCF^P35.2

abdominal sternite | pharate adult stage (with CTCF^P35.2)

abdominal sternite | pharate adult stage (with CTCF^y+1)

CTCF^P35.2

abdominal sternite bristle

abdominal sternite bristle (with CTCF^EY15833)

CTCF^y+1

abdominal sternite bristle (with CTCF^GE24185)

CTCF^y+1

abdominal sternite bristle (with CTCF^y+1)

abdominal sternite bristle (with Df(3L)0463)

CTCF^GE24185

abdominal sternite bristle | pharate adult stage (with CTCF⁹)

CTCF^P35.2

abdominal sternite bristle | pharate adult stage (with CTCF^P35.2)

abdominal sternite bristle | pharate adult stage (with CTCF^y+1)

CTCF^P35.2

abdominal tergite

abdominal tergite 4

abdominal tergite 4 (with Df(3L)0463)

CTCF^P30.6

abdominal tergite 5

abdominal tergite 5 (with Df(3L)0463)

abdominal tergite 7

abdominal tergite 7 (with Df(3L)0463)

CTCF^P30.6

abdominal tergite 7 & abdominal sternite bristle

abdominal tergite 7 & abdominal sternite bristle (with Df(3L)0463)

CTCF^GE24185

abdominal tergite (with CTCF^EY15833)

CTCF^y+1

abdominal tergite (with CTCF^GE24185)

CTCF^y+1

abdominal tergite (with CTCF^y+1)

abdominal tergite | pharate adult stage (with CTCF⁹)

CTCF^P35.2

abdominal tergite | pharate adult stage (with CTCF^P35.2)

abdominal tergite | pharate adult stage (with CTCF^y+1)

CTCF^P35.2

adult external abdomen (with CTCF^attP.mCh)

adult external abdomen (with CTCF^attP)

CTCF^GE24185

adult external abdomen (with CTCF^C1)

CTCF^attP.mCh

adult external abdomen (with CTCF^GE24185)

adult external abdomen | adult stage

adult external abdomen | adult stage (with CTCF^attP)

CTCF^C1

adult external abdomen | adult stage (with CTCF^C1)

CTCF^attP

adult external abdomen | P-stage

adult external abdomen | P-stage (with CTCF^attP.mCh)

CTCF^C2

adult external abdomen | P-stage (with CTCF^C2)

CTCF^attP.mCh

embryonic/larval salivary gland | larval stage, with Scer\GAL4^nub.PU

CTCF^GD5052

eye, with Scer\GAL4^ey.PH

CTCF^GL00266

female genitalia | adult stage (with CTCF^attP)

CTCF^GE24185

female genitalia | adult stage (with CTCF^C2)

CTCF^GE24185

female genitalia | adult stage (with CTCF^GE24185)

genitalia | adult stage

genitalia | adult stage (with CTCF^attP.mCh)

genitalia | adult stage (with CTCF^C1)

genitalia | adult stage (with CTCF^C2)

CTCF^C1

genitalia | adult stage (with CTCF^GE24185)

genitalia | P-stage

genitalia | P-stage (with CTCF^attP.mCh)

CTCF^C2

genitalia | P-stage (with CTCF^C2)

CTCF^attP.mCh

hypogynium | adult stage

CTCF^GE24185

male genitalia

male genitalia (with CTCF^EY15833)

CTCF^y+1

male genitalia (with CTCF^GE24185)

CTCF^y+1

male genitalia (with CTCF^y+1)

male genitalia (with Df(3L)0463)

male genitalia | pharate adult stage (with CTCF⁹)

CTCF^P35.2

male genitalia | pharate adult stage (with CTCF^P35.2)

male genitalia | pharate adult stage (with CTCF^y+1)

CTCF^P35.2

myofibril, with Scer\GAL4^Mef2.PR

CTCF^GD5052

nucleus | larval stage, with Scer\GAL4^nub.PU

CTCF^GD5052

posterior crossvein, with Scer\GAL4^en.PU

CTCF^GL00266

sarcomere, with Scer\GAL4^Mef2.PR

CTCF^GD5052

wing

wing, with Scer\GAL4^Bx-MS1096

CTCF^{UAS.ORF.GW.Tag:HA}

wing (with Df(3L)0463)

CTCF^GE24185

Z disc, with Scer\GAL4^Mef2.PR

CTCF^GD5052

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (221)

Hsap\CTCFL

7 of 14

Yes

Hsap\CTCF

6 of 14

Yes

Hsap\ZBTB48

2 of 14

Yes

Hsap\ZNF358

2 of 14

Yes

Hsap\ZNF692

2 of 14

Yes

Hsap\BACH1

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Hsap\LOC728743

1 of 14

Yes

Hsap\MAZ

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ZBTB7A

1 of 14

Hsap\ZBTB17

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ZBTB44

1 of 14

Hsap\ZBTB45

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ZNF267

1 of 14

Hsap\ZNF268

1 of 14

Hsap\ZNF276

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ZNF418

1 of 14

Hsap\ZNF425

1 of 14

Hsap\ZNF426

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ZNF484

1 of 14

Hsap\ZNF493

1 of 14

Hsap\ZNF517

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Hsap\ZNF660-ZNF197

1 of 14

Hsap\ZNF665

1 of 14

Hsap\ZNF668

1 of 14

Yes

Hsap\ZNF671

1 of 14

Hsap\ZNF672

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Hsap\ZNF765-ZNF761

1 of 14

Hsap\ZNF766

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ZSCAN12

1 of 14

Hsap\ZSCAN31

1 of 14

Hsap\ZSCAN32

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (27)

Rnor\Ctcfl

6 of 14

Yes

Rnor\Ctcf

6 of 14

Yes

Rnor\Zbtb48

2 of 14

Yes

Rnor\E4f1

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Zbtb49

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Znf768

1 of 14

Yes

Mus musculus (laboratory mouse) (43)

Mmus\Ctcfl

7 of 14

Yes

Mmus\Ctcf

6 of 14

Yes

Mmus\2010315B03Rik

1 of 14

Mmus\AI854703

1 of 14

Yes

Mmus\E4f1

1 of 14

Yes

Mmus\Gm2381

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Zfp354c

1 of 14

Yes

Mmus\Zfp410

1 of 14

Yes

Mmus\Zfp524

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Zfp746

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (66)

Xtro\ctcf

2 of 13

Yes

Xtro\bcl6

1 of 13

Yes

Xtro\cxcl9

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\ikzf3

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\zbtb20

1 of 13

Yes

Xtro\zbtb38

1 of 13

Yes

Xtro\zbtb41

1 of 13

Xtro\zbtb44

1 of 13

Yes

Xtro\zbtb47

1 of 13

Yes

Xtro\zfat

1 of 13

Xtro\zfp64

1 of 13

Yes

Xtro\zfx

1 of 13

Yes

Xtro\znf84

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\znf282

1 of 13

Xtro\znf319

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (26)

Drer\ctcf

6 of 14

Yes

Drer\LOC100150619

1 of 14

Drer\LOC100535691

1 of 14

Yes

Drer\LOC101883151

1 of 14

Yes

Drer\LOC108190743

1 of 14

Yes

Drer\pogza

1 of 14

Drer\pogzb

1 of 14

Drer\si:ch73-158p21.7

1 of 14

Drer\si:dkey-34m19.3

1 of 14

Drer\si:dkeyp-53d3.6

1 of 14

Drer\si:dkeyp-121d2.7

1 of 14

Drer\vezf1a

1 of 14

Yes

Drer\vezf1b

1 of 14

Drer\zbtb16a

1 of 14

Yes

Drer\zbtb16b

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\znf648

1 of 14

Yes

Drer\znf740a

1 of 14

Yes

Drer\znf983

1 of 14

Drer\znf1042

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (8)

Cele\ehn-3

1 of 14

Yes

Cele\F21A9.2

1 of 14

Yes

Cele\hinf-1

1 of 14

Yes

Cele\sprp-4

1 of 14

Yes

Cele\Y5F2A.4

1 of 14

Yes

Cele\Y17G7B.22

1 of 14

Yes

Cele\ZK177.3

1 of 14

Yes

Cele\znf-782

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (37)

Agam\AgaP_AGAP005555

7 of 12

Yes

Agam\AgaP_AGAP003056

2 of 12

Yes

Agam\AgaP_AGAP000286

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Agam\AgaP_AGAP005568

1 of 12

Agam\AgaP_AGAP006129

1 of 12

Yes

Agam\AgaP_AGAP006544

1 of 12

Yes

Agam\AgaP_AGAP007516

1 of 12

Agam\AgaP_AGAP008232

1 of 12

Agam\AgaP_AGAP008429

1 of 12

Yes

Agam\AgaP_AGAP008795

1 of 12

Yes

Agam\AgaP_AGAP008796

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Agam\AgaP_AGAP010751

1 of 12

Agam\AgaP_AGAP011262

1 of 12

Agam\AgaP_AGAP011758

1 of 12

Yes

Agam\AgaP_AGAP011759

1 of 12

Agam\AgaP_AGAP011763

1 of 12

Yes

Agam\AgaP_AGAP012110

1 of 12

Agam\AgaP_AGAP012598

1 of 12

Yes

Agam\AgaP_AGAP013275

1 of 12

Arabidopsis thaliana (thale-cress) (2)

Atha\AT3G29340

1 of 13

Yes

Atha\YY1

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (4)

Scer\AZF1

2 of 13

Yes

Scer\STP3

1 of 13

Yes

Scer\STP4

1 of 13

Yes

Scer\ZAP1

1 of 13

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:CTCF. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (58)

CG14655

2 of 13

Kr-h1

2 of 13

Odj

2 of 13

Cf2

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Cph

1 of 13

crol

1 of 13

dwg

1 of 13

her

1 of 13

Hinfp

1 of 13

Imzf

1 of 13

jim

1 of 13

Nnk

1 of 13

pad

1 of 13

Paris

1 of 13

Phs

1 of 13

pita

1 of 13

rgr

1 of 13

row

1 of 13

sens

1 of 13

sqz

1 of 13

su(Hw)

1 of 13

topi

1 of 13

trabl

1 of 13

ush

1 of 13

wdn

1 of 13

Zaf1

1 of 13

Zif

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

intellectual disability, autosomal dominant 21

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 2 )

Allele

Disease

Evidence

References

CTCF^EY15833

model of autosomal dominant intellectual developmental disorder 21

CEA

(Konrad et al., 2019)

CTCF^KK100986

model of autosomal dominant intellectual developmental disorder 21

CEA

(Konrad et al., 2019)

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

CTCF; CCCTC-binding factor

model of autosomal dominant intellectual developmental disorder 21

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CTCFL; CCCTC-binding factor like

7 of 14

607022

CTCF; CCCTC-binding factor

6 of 14

604167

INTELLECTUAL DEVELOPMENTAL DISORDER, AUTOSOMAL DOMINANT 21; MRD21

autosomal dominant intellectual developmental disorder 21

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

CTCF

molecular sieving, molecular weight estimation by staining, anti tag coimmunoprecipitation, anti tag western blot, bimolecular fluorescence complementation, fluorescence microscopy, protein cross-linking with a bifunctional reagent, cross-linking study, two hybrid array, two hybrid, light scattering

(Kamalyan et al., 2024, Bonchuk et al., 2020, Shokri et al., 2019, Bonchuk et al., 2015)

CTCF - AGO2

affinity chromatography technology, western blot

(Moshkovich et al., 2011)

CTCF - Ada2b

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

CTCF - CG1737

pull down, Identification by mass spectrometry

(Kaushal et al., 2022)

CTCF - Caf1-55

anti tag coimmunoprecipitation, anti bait coimmunoprecipitation, western blot

(Bohla et al., 2014)

CTCF - Chro

anti tag coimmunoprecipitation, anti bait coimmunoprecipitation, western blot

(Bohla et al., 2014)

CTCF - EcR

anti bait coimmunoprecipitation, western blot

(Pascual-Garcia et al., 2017)

CTCF - HIPP1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Alekseyenko et al., 2014)

CTCF - Hr78

two hybrid array

(Shokri et al., 2019)

CTCF - Ibf1

pull down, Identification by mass spectrometry

(Kaushal et al., 2022)

CTCF - Ibf2

pull down, Identification by mass spectrometry

(Kaushal et al., 2022)

CTCF - Iswi

anti tag coimmunoprecipitation, anti bait coimmunoprecipitation, western blot

(Bohla et al., 2014)

CTCF - Map60

pull down, molecular weight estimation by staining, Identification by mass spectrometry

(Kaushal et al., 2022)

CTCF - Myb

anti tag coimmunoprecipitation, anti bait coimmunoprecipitation, western blot

(Bohla et al., 2014)

CTCF - Nlp

anti bait coimmunoprecipitation, western blot

(Padeken et al., 2013)

CTCF - Nup98-96

anti bait coimmunoprecipitation, western blot

(Pascual-Garcia et al., 2017)

CTCF - SA1

pull down, Identification by mass spectrometry, molecular weight estimation by staining

(Kaushal et al., 2022, Kaushal et al., 2021)

CTCF - SMC1

pull down, Identification by mass spectrometry

(Kaushal et al., 2022)

CTCF - SMC3

pull down, Identification by mass spectrometry

(Kaushal et al., 2022)

CTCF - Sgf11

pull down, molecular weight estimation by staining

(Bonchuk et al., 2016)

CTCF - Ubx

bimolecular fluorescence complementation, fluorescence microscopy

(Bischof et al., 2018)

CTCF - abd-A

bimolecular fluorescence complementation, fluorescence microscopy

(Bischof et al., 2018)

CTCF - cid

anti tag coimmunoprecipitation, western blot

(Padeken et al., 2013)

CTCF - e(y)2

pull down, western blot, molecular weight estimation by staining, anti bait coimmunoprecipitation, two hybrid

(Bonchuk et al., 2016, Maksimenko et al., 2014)

CTCF - mip120

anti tag coimmunoprecipitation, anti bait coimmunoprecipitation, western blot

(Bohla et al., 2014)

CTCF - mip130

anti tag coimmunoprecipitation, anti bait coimmunoprecipitation, western blot

(Bohla et al., 2014)

CTCF - mod

anti bait coimmunoprecipitation, western blot

(Padeken et al., 2013)

CTCF - pzg

anti tag coimmunoprecipitation, anti bait coimmunoprecipitation, western blot

(Bohla et al., 2014)

CTCF - vtd

pull down, molecular weight estimation by staining, Identification by mass spectrometry

(Kaushal et al., 2022, Kaushal et al., 2021)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

CTCF

enhanceable

mod(mdg4)

(Savitsky et al., 2016)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

mod(mdg4)

enhanceable

CTCF

(Savitsky et al., 2016)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

64257

DroID - A comprehensive database of gene and protein interactions.

FBgn0035769

MIST (protein-protein) - An integrated Molecular Interaction Database

38817

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Generic Transcription Pathway

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9VS55

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-20

Cytogenetic map

65F6-65F6

Sequence location

3L:7,353,578..7,356,713 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

65F6-65F6

Limits computationally determined from genome sequence between P{EP}lark^EP3259&P{lacW}l(3)j1D5^j1D5 and P{PZ}l(3)07217⁰⁸²²³&P{EP}Rac2^EP3118

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-20

(FlyBase, 2016)

3-17.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (15)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

604736

y¹ v¹; P{TOE.GS03002}attP40

40850

y¹ v¹; P{TRiP.HMS02017}attP40

64810

y¹ w^*; P{CTCF-GFP.FPTB}attP40

21162

y¹ w^67c23; P{EPgy2}CTCF^EY15833

33480

y¹ w^*; CTCF³⁰/TM6B, Tb¹

59963

y¹ w^*; CTCF^y+1/TM6B, Tb¹

35354

y¹ sc^* v¹ sev²¹; P{TRiP.GL00266}attP2

FlyORF

F004800

M{UAS-CTCF.ORF-VN}ZH-86Fb

F000619

M{UAS-CTCF.ORF.3xHA.GW}ZH-86Fb

VDRC

v108857

P{KK100986}VIE-260B

v30713

w¹¹¹⁸; P{GD5052}v30713

v341372

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD00430}attP40/CyO-GFP

More stocks available...

Genomic Clones (16)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (40)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH14774

Other clones

DPiM_GH14774

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0035769

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0035769

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Kaushal et al., 2021, Maksimenko et al., 2020, Bonchuk et al., 2015, Maksimenko et al., 2015, Maksimenko et al., 2014, Smith et al., 2009, Gerasimova et al., 2007)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

CTCF19.7-1c

Other Stable Cell Lines

New stable cell line derived from S2-unspecified : S2 cells were stably transfected with CTCF flagged with Tag:FLAG.
(Mohan et al., 2007)

Other Comments

DNA-protein interactions: genome-wide binding profile assayed for CTCF protein in Kc167 cells; see Chromatin_types_NKI collection report. Individual protein-binding experiments listed under "Samples" at GEO_GSE22069 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE22069).

(Filion et al., 2010)

DNA-protein interactions: genome-wide binding profile assayed for CTCF protein in 0-12 hr embryos; see Insulator_Class_I.mE01 collection report. Individual protein-binding experiments listed under "Samples" at GEO: 16245 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE16245); included are assays in Kc and S2 cell lines.

(Nègre et al., 2010)

Many, but not all CTCF binding sites depend on Cp190.

(Mohan et al., 2007)

CTCF mutants show a homeotic phenotype and pharate lethality.

(Mohan et al., 2007)

Relationship to Other Genes

Source for database merge of

Additional comments

Source for identity of CTCF CG8591 was sequence comparison ( date:020710 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: CTCF CG8591

(FlyBase, 1996-)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

CG8591

(Ni et al., 2011.7.19, Ni et al., 2010.12.1, Keleman et al., 2009.8.5)

CTCF

(Dolsten et al., 2025, Jayakrishnan et al., 2025, Sigalova et al., 2025, Jayakrishnan et al., 2024, Li and Levine, 2024, Pollex et al., 2024, Tullius et al., 2024, Zhang et al., 2024, Gaskill et al., 2023, Kahn et al., 2023, Messina et al., 2023, Chetverina et al., 2022, Ding et al., 2022, Kamiyama and Niwa, 2022, Kaushal et al., 2022, Richards et al., 2022, Bilder et al., 2021, Chetverina et al., 2021, Erokhin et al., 2021, Kaushal et al., 2021, Khalisova et al., 2021, Kyrchanova et al., 2021, Llorens-Giralt et al., 2021, Maksimenko et al., 2021, Wolfe et al., 2021, Bender, 2020, Bonchuk et al., 2020, De et al., 2020, Fresán et al., 2020, Khaminets et al., 2020, Luo et al., 2020, Melnikova et al., 2020, Meyer-Nava et al., 2020, Nora et al., 2020, Rozenwald et al., 2020, Santana et al., 2020, Srinivasan and Mishra, 2020, Szabo et al., 2020, Cardozo Gizzi et al., 2019, Chen and Lei, 2019, Cheutin and Cavalli, 2019, Henderson et al., 2019, Konrad et al., 2019, Kyrchanova et al., 2019, Luzhin et al., 2019, Matthews and White, 2019, Shokri et al., 2019, Viets et al., 2019, Vorobyeva and Mazina, 2019, Zykova et al., 2019, Bischof et al., 2018, Gambetta and Furlong, 2018, Gene Disruption Project members, 2018-, Lu et al., 2018, Prange et al., 2018, Rennie et al., 2018, Ciabrelli et al., 2017, El-Sharnouby et al., 2017, Gerland et al., 2017, Lou et al., 2017, Melnikova et al., 2017, Pascual-Garcia et al., 2017, Transgenic RNAi Project members, 2017-, Zaytseva and Quinn, 2017, Kwon et al., 2016, Rowley and Corces, 2016, Savitsky et al., 2016, Dai et al., 2015, Dietz et al., 2015, Li et al., 2015, Magbanua et al., 2015, Maksimenko et al., 2015, model organism Encyclopedia of Regulatory Network (modERN) Project, 2015-, Nadimpalli et al., 2015, Naval-Sánchez et al., 2015, Nikalayevich and Ohkura, 2015, Saberi et al., 2015, Schertel et al., 2015, Singh and Mishra, 2015, Van Bortle et al., 2015, Alekseyenko et al., 2014, Bowman et al., 2014, Boyle et al., 2014, Comoglio and Paro, 2014, Cuartero et al., 2014, Ho et al., 2014, Jiang and Singh, 2014, Maksimenko et al., 2014, McElroy et al., 2014, Navarro et al., 2014, Pascual-Garcia et al., 2014, Pathak et al., 2014, Slattery et al., 2014, Villar et al., 2014, Li et al., 2013, Ong et al., 2013, Padeken et al., 2013, Rohrbaugh et al., 2013, Rubin and Green, 2013, Schuettengruber and Cavalli, 2013, Fabian and Brill, 2012, Feller et al., 2012, Follmer et al., 2012, Hou et al., 2012, McEachern, 2012, Ni et al., 2012, Philip et al., 2012, Sakoparnig et al., 2012, Schwartz et al., 2012, Alic et al., 2011, Demakov et al., 2011, Guerrero and Maggert, 2011, Li et al., 2011, Nègre et al., 2011, Pruteanu-Malinici et al., 2011, Richter et al., 2011, Sultana et al., 2011, Weake et al., 2011, Cook et al., 2010.2.12, Filion et al., 2010, Guertin and Lis, 2010, Kalverda and Fornerod, 2010, Kong et al., 2010, Kong et al., 2010, Macdonald et al., 2010, Meller and Lloyd, 2010.12.13, modENCODE Consortium et al., 2010, Oliver et al., 2010, van Bemmel et al., 2010, Wallace et al., 2010, Bushey et al., 2009, Jiang et al., 2009, Smith et al., 2009, Chetverina et al., 2008, Chen et al., 2007, Ciavatta et al., 2007, Holohan et al., 2007, Rathke et al., 2007, Moon et al., 2005, Franchini et al., 2004, Stanyon et al., 2004)

dCTCF

(Kamalyan et al., 2024, Sokolov et al., 2024, Golovnin et al., 2023, Kyrchanova et al., 2023, Albright et al., 2022, Benoit et al., 2022, Kyrchanova et al., 2022, Peterson et al., 2021, Sabirov et al., 2021, Arzate-Mejía et al., 2020, Dong et al., 2020, Heurteau et al., 2020, Kyrchanova et al., 2020, Maksimenko et al., 2020, Glenn and Geyer, 2019, Kostyuchenko et al., 2019, Postika et al., 2019, Fedoseeva et al., 2018, Mourad and Cuvier, 2018, Ong and Lin, 2018, Pokholkova et al., 2018, Postika et al., 2018, Wang et al., 2018, Heseding et al., 2017, Mourad et al., 2017, Bonchuk et al., 2016, Khoroshko et al., 2016, Kyrchanova et al., 2016, Ulianov et al., 2016, Bonchuk et al., 2015, Golovnin et al., 2015, Maksimenko et al., 2015, Ahanger et al., 2014, Korenjak et al., 2014, Kyrchanova and Georgiev, 2014, Maksimenko et al., 2014, McElroy et al., 2014, Van Bortle et al., 2014, Fedoseeva and Tchurikov, 2013, Gurudatta et al., 2013, Kellner et al., 2013, Li and Zhou, 2013, Ong et al., 2013, Van Bortle et al., 2012, Kyrchanova et al., 2011, Rach et al., 2011, Ramos et al., 2011, Vogelmann et al., 2011, Wood et al., 2011, Macdonald et al., 2010, Nègre et al., 2010, Schoborg and Labrador, 2010, Bartkuhn et al., 2009, Chetverina et al., 2008, Kyrchanova et al., 2008, Kyrchanova et al., 2008, Li et al., 2008, Pérez-Lluch et al., 2008, Ciavatta et al., 2007, Gerasimova et al., 2007, Mohan et al., 2007, Smith et al., 2007, Rasheva et al., 2005)

Name Synonyms

CCCTC-Binding Factor

(Guerrero and Maggert, 2011)

CCCTC-binding factor

(Magbanua et al., 2015, Comoglio and Paro, 2014)

CTCF

(Gerasimova et al., 2007, Holohan et al., 2007)

Secondary FlyBase IDs

Datasets (6)

Study focus (6)

Experimental Role

Project

Project Type

Title

transgene_used

FBGSE20313

genome binding

The insulator protein SU(HW) modulates nuclear lamina interactions of the Drosophila genome.

transgene_used

FBGSE22069

genome binding

Protein profiling reveals five principal chromatin types in Drosophila cells.

RNAi_target

modENCODE_chromatin_proteins_cells

genome binding

Genome-wide localization of chromosomal proteins in cell lines by ChIP-chip and ChIP-Seq.

bait_protein

modENCODE_chromatin_proteins_flies

genome binding

Genome-wide localization of chromosomal proteins in fly tissues by ChIP-chip and ChIP-Seq.

RNAi_target

modENCODE_chromatin_histones_cells

genome binding

Genome-wide localization of histones and their modifications in cell lines by ChIP-chip and ChIP-Seq.

bait_protein

modENCODE_regulation_insulators

genome binding

Genome-wide localization of insulator proteins by ChIP-chip and ChIP-Seq.

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 44 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38817

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VS55

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VS55

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0035769

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38817/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0035769

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0035769

FlyMine - An integrated database for Drosophila genomics

FBgn0035769

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG8591

MARRVEL_MODEL - MARRVEL (model organism gene)

38817

Linkouts

BioGRID - A database of protein and genetic interactions.

64257

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0035769

DroID - A comprehensive database of gene and protein interactions.

FBgn0035769

DRSC - Results frm RNAi screens

FBgn0035769

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CTCF_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0035769

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0035769

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8591

Flygut - An atlas of the Drosophila adult midgut

FBgn0035769

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC002961

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

38817

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Generic Transcription Pathway

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9VS55

References (257)

The following external sites may use different assemblies or annotations than FlyBase.

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