FlyBase Gene Report: Dmel\zen2

General Information

Symbol

Dmel\zen2

Species

D. melanogaster

Name

zerknullt-related

Annotation Symbol

CG1048

Feature Type

protein_coding_gene

FlyBase ID

FBgn0004054

Gene Model Status

Current

Stock Availability

9 publicly available

Gene Summary

zerknullt-related (zen2) encodes a member of the Antennapedia Complex (ANT-C), one of two Hox gene complexes. zen2 is similar in sequence and expression to the adjacent zen gene. The two are closely linked and they appear to be duplicate copies with zen being the ancestral member of the pair. Despite its similarity to zen, absence of zen2 function has no discernible effect on development or morphology. [Date last reviewed: 2019-03-21] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

Key Links

Genomic Location

Cytogenetic map

84A5-84A5

Sequence location

3R:6,743,135..6,744,136 [-]

Recombination map

3-48

RefSeq locus

NT_033777 REGION:6743135..6744136

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (6 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables DNA binding

inferred from electronic annotation with InterPro:IPR001356, InterPro:IPR020479

(InterPro Project Members, 2004-)

enables DNA-binding transcription factor activity, RNA polymerase II-specific

inferred from biological aspect of ancestor with PANTHER:PTN005228379

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR017970

(InterPro Project Members, 2004-)

enables RNA polymerase II cis-regulatory region sequence-specific DNA binding

inferred from biological aspect of ancestor with PANTHER:PTN005228379

(GO Reference Genome Project, 2011-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in regulation of DNA-templated transcription

inferred from electronic annotation with InterPro:IPR017970

(InterPro Project Members, 2004-)

involved_in regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN005228379

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN005228379

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

HOX-LIKE HOMEOBOX TRANSCRIPTION FACTORS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

HOX-LIKE HOMEOBOX TRANSCRIPTION FACTORS -: HOX-like (HOXL) homeobox transcription factors are sequence-specific DNA binding proteins that regulate transcription. They encompass transcription factors encoded by the Hox genes of the Antennapedia and the Bithorax gene complexes and genes closely related in sequence. HOXL transcription factors are major regulators of animal development. (Adapted from FBrf0232555).

Protein Function (UniProtKB)

Required for the differentiation of the dorsal-ventral pattern, and does not appear to be involved in the process of segmentation.

(UniProt, P09090)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

z2: zen-2

Simultaneous deletion of both z2 and pb produces only a pb mutant phenotype. Thus absence of z2 function has no discernible effect on development or morphology.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\zen2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P09090)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.49

Gene model reviewed during 5.50

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

zen2-RA

FBtr0081671

NM_057446

935

252

zen2-RB

FBtr0290296

NM_001144545

940

232

Additional Transcript Data and Comments

Reported size (kB)

1.1 (northern blot)

(Pultz et al., 1988)

0.9 (northern blot)

(Rushlow et al., 1987)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

zen2-PA

FBpp0081171

29.0

252

4.69

FBpp0288735

26.7

232

4.99

Q0IGT0

NP_001138017

ACL83476

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

252 (aa); 28 (kD)

(Rushlow et al., 1987)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\zen2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

zen2_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

NA

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 4 -- 6

dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

foregut anlage in statu nascendi

(Fisher et al., 2012)

posterior ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

gastrula stage

organism | dorsal

(Rushlow et al., 1987)

embryonic stage 7 -- 8

amnioserosa anlage

(Fisher et al., 2012)

embryonic cycle 14

organism | anterior | dorsal

(Rushlow et al., 1987)

extended germ band stage

amnioserosa

(Rushlow et al., 1987)

dorsal ectoderm | restricted

(Rushlow et al., 1987)

embryonic stage 9

dorsal fold

Comment: reference states 3.5 hr AEL

(Pultz et al., 1988)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(Pultz et al., 1988, Rushlow et al., 1987)

embryonic stage 1 -- 6

organism

(Pultz et al., 1988)

Additional Descriptive Data

zen2 transcripts are observed between 0 and 6 hrs of embryonic development with a peak at 2-4hrs on northern blots. No zen2 transcripts are observed in postembryonic stages. zen2 transcripts are observed along the dorsal surface of the pre-cellular blastoderm embryo by in situ hybridization. As gastrulation proceeds, they become restricted to the area of the dorsal folds.

(Pultz et al., 1988)

Northern analysis of RNA from 0-24 hour embryos indicate that zen2 transcript is expressed in early embryos, and expression peaks at 2-3 hours of embryogenesis. Using radioactive in situ hybridization, zen2 expression is detected in the dorsal surface and the anterior and posterior poles of embryonic cycle 14 embryos. By gastrulation, zen2 expression is restricted to the dorsal surface. During germ band elongation, zen2 transcript is detected in the amnioserosa and a region of the dorsal ectoderm corresponding to the presumptive ptic lobe.

(Rushlow et al., 1987)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\zen2 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\zen2

View exonic expression by tissue for Dmel\zen2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0004054

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40827/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004054

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004054

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004054

Flygut - An atlas of the Drosophila adult midgut

FBgn0004054

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\zen2

Stages(s) 4-6
Stages(s) 7-8

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 20 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of zen2

Transgenic constructs containing regulatory region of zen2

Aberrations (Deficiencies and Duplications) ( 11 )

Inferred from experimentation ( 11 )

Gene disrupted in

Df(3R)Antp-RX2

(Kaufman et al., 1990)

Df(3R)MAP8

(Kaufman et al., 1990)

Df(3R)MAP13

(Kaufman et al., 1990)

Df(3R)MAP14

(Kaufman et al., 1990)

Df(3R)pb-X2

(Kaufman et al., 1990)

Df(3R)WIN1

(Kaufman et al., 1990)

Df(3R)WIN3

(Kaufman et al., 1990)

Gene not disrupted in

Df(3R)LIN

(Kaufman et al., 1990)

Df(3R)MAP2

(Kaufman et al., 1990)

Df(3R)MAP117

(Kaufman et al., 1990)

Df(3R)SCB-XL2

(Kaufman, 1994.10.5, Cribbs et al., 1992, Kaufman et al., 1990)

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^pnr-MD237

zen2^GD4144

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

zen2^GD4144

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

zen2^GD4144

Phenotype manifest in

Allele

larval somatic muscle cell, with Scer\GAL4^Mef2.PR

zen2^GD4144

sarcomere, with Scer\GAL4^Mef2.PR

zen2^GD4144

Z disc, with Scer\GAL4^Mef2.PR

zen2^GD4144

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (155)

Hsap\HOXA4

4 of 14

Yes

Hsap\HOXA5

4 of 14

Yes

Hsap\HOXA6

4 of 14

Yes

Hsap\HOXA7

4 of 14

Yes

Hsap\HOXB4

4 of 14

Yes

Hsap\HOXB5

4 of 14

Yes

Hsap\HOXB6

4 of 14

Yes

Hsap\HOXB7

4 of 14

Yes

Hsap\HOXB8

4 of 14

Yes

Hsap\HOXC4

4 of 14

Yes

Hsap\HOXC5

4 of 14

Yes

Hsap\HOXC6

4 of 14

Yes

Hsap\HOXC8

4 of 14

Yes

Hsap\HOXD4

4 of 14

Yes

Hsap\HOXD8

4 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\DUX4L7

1 of 14

Yes

Hsap\DUX4L9

1 of 14

Yes

Hsap\DUXA

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\HOXD12

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (111)

Rnor\Hoxa6

4 of 14

Yes

Rnor\Hoxa7

4 of 14

Yes

Rnor\Hoxb4

4 of 14

Yes

Rnor\Hoxb5

4 of 14

Yes

Rnor\Hoxb6

4 of 14

Yes

Rnor\Hoxb7

4 of 14

Yes

Rnor\Hoxc5

4 of 14

Yes

Rnor\Hoxc6

4 of 14

Yes

Rnor\Hoxc8

4 of 14

Yes

Rnor\Hoxd3

4 of 14

Yes

Rnor\Hoxd4

4 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Hoxa11

1 of 14

Rnor\Hoxc11

1 of 14

Rnor\Hoxd12

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (110)

Mmus\Hoxa4

4 of 14

Yes

Mmus\Hoxa5

4 of 14

Yes

Mmus\Hoxa6

4 of 14

Yes

Mmus\Hoxa7

4 of 14

Yes

Mmus\Hoxb4

4 of 14

Yes

Mmus\Hoxb5

4 of 14

Yes

Mmus\Hoxb7

4 of 14

Yes

Mmus\Hoxb8

4 of 14

Yes

Mmus\Hoxc4

4 of 14

Yes

Mmus\Hoxc5

4 of 14

Yes

Mmus\Hoxc6

4 of 14

Yes

Mmus\Hoxc8

4 of 14

Yes

Mmus\Hoxd4

4 of 14

Yes

Mmus\Hoxd8

4 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Mmus\Meox1

3 of 14

Mmus\Mnx1

3 of 14

Mmus\Pdx1

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Rhox3f

1 of 14

Yes

Mmus\Rhox3h

1 of 14

Yes

Mmus\Rhox12

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (155)

Xtro\LOC101731251

4 of 13

Yes

3 of 13

3 of 13

3 of 13

3 of 13

Yes

Xtro\hoxb5

3 of 13

Xtro\hoxb8

3 of 13

Xtro\hoxc3

3 of 13

Yes

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Yes

Xtro\hoxb2

2 of 13

Xtro\hoxb3

2 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Xtro\homeobox100496651-provisional

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\hoxc13

1 of 13

Yes

Xtro\hoxd13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\soho1

1 of 13

Yes

Xtro\synpo2l

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (160)

Drer\hoxb4a

4 of 14

Yes

Drer\hoxb5a

4 of 14

Yes

Drer\hoxb5b

4 of 14

Yes

Drer\hoxb6a

4 of 14

Yes

Drer\hoxb6b

4 of 14

Yes

Drer\hoxb7a

4 of 14

Yes

Drer\hoxb8a

4 of 14

Yes

Drer\hoxb8b

4 of 14

Yes

Drer\hoxc3a

4 of 14

Yes

Drer\hoxc4a

4 of 14

Yes

Drer\hoxc8a

4 of 14

Yes

Drer\hoxd4a

4 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

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1 of 14

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1 of 14

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1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

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1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (52)

Cele\lin-39

4 of 14

Yes

Cele\mab-5

4 of 14

Yes

Cele\ceh-12

3 of 14

Cele\ceh-13

3 of 14

Yes

Cele\ceh-63

3 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (64)

Agam\AgaP_AGAP004647

5 of 12

Yes

4 of 12

4 of 12

4 of 12

4 of 12

4 of 12

4 of 12

4 of 12

4 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (47)

Atha\HB-1

5 of 13

Yes

Atha\HB5

5 of 13

Yes

Atha\HB6

5 of 13

Yes

Atha\HB-7

5 of 13

Yes

Atha\HB16

5 of 13

Yes

Atha\ATHB13

4 of 13

Yes

Atha\AtHB23

4 of 13

Yes

Atha\ATHB54

4 of 13

Atha\HB-3

4 of 13

Yes

Atha\HB-12

4 of 13

Yes

Atha\HB20

4 of 13

Yes

Atha\HB22

4 of 13

Atha\HB51

4 of 13

Atha\HAT1

3 of 13

Yes

Atha\HAT2

3 of 13

Yes

Atha\HAT3

3 of 13

Yes

Atha\HAT9

3 of 13

Yes

Atha\HAT14

3 of 13

Yes

Atha\HAT22

3 of 13

Yes

Atha\HB-2

3 of 13

Yes

Atha\HB4

3 of 13

Yes

Atha\HB17

3 of 13

Yes

Atha\HB18

3 of 13

Yes

Atha\HB21

3 of 13

Yes

Atha\HB40

3 of 13

Yes

Atha\HB52

3 of 13

Yes

Atha\HB53

3 of 13

Yes

Atha\ANL2

1 of 13

Atha\AT4G03250

1 of 13

Atha\ATHB-15

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\YHP1

3 of 13

Yes

Scer\YOX1

3 of 13

Yes

Scer\PHO2

1 of 13

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\phx1

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:zen2. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (85)

7 of 13

zen

7 of 13

bcd

6 of 13

btn

6 of 13

Dfd

6 of 13

ftz

6 of 13

lab

6 of 13

Scr

6 of 13

5 of 13

5 of 13

5 of 13

5 of 13

cad

4 of 13

Dbx

4 of 13

ems

4 of 13

HGTX

4 of 13

ind

4 of 13

lms

4 of 13

Ubx

4 of 13

Abd-B

3 of 13

bap

3 of 13

bsh

3 of 13

CG18599

3 of 13

CG34031

3 of 13

eve

3 of 13

H2.0

3 of 13

HHEX

3 of 13

Hmx

3 of 13

inv

3 of 13

NK7.1

3 of 13

slou

3 of 13

tin

3 of 13

2 of 13

B-H1

2 of 13

B-H2

2 of 13

C15

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Dll

2 of 13

Drgx

2 of 13

gsb

2 of 13

gsb-n

2 of 13

Gsc

2 of 13

hbn

2 of 13

lbe

2 of 13

lbl

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

tup

2 of 13

unc-4

2 of 13

vnd

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Awh

1 of 13

CG30480

1 of 13

CG32532

1 of 13

exd

1 of 13

nub

1 of 13

onecut

1 of 13

otp

1 of 13

prd

1 of 13

Tes

1 of 13

toe

1 of 13

toy

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 4 )

Human Ortholog

Disease

Evidence

References

PDX1; pancreatic and duodenal homeobox 1

model of pancreatic agenesis 1

IEA

(FlyBase, 2019-)

model of maturity-onset diabetes of the young type 4

IEA

(FlyBase, 2019-)

model of type 2 diabetes mellitus

IEA

(FlyBase, 2019-)

PAX4; paired box 4

model of type 2 diabetes mellitus

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

GBX1; gastrulation brain homeobox 1

3 of 14

603354

GBX2; gastrulation brain homeobox 2

3 of 14

3 of 14

3 of 14

DIENCEPHALIC-MESENCEPHALIC JUNCTION DYSPLASIA SYNDROME 2; DMJDS2

HOXA2; homeobox A2

3 of 14

604685

MICROTIA, HEARING IMPAIRMENT, AND CLEFT PALATE

3 of 14

3 of 14

FACIAL PARESIS, HEREDITARY CONGENITAL, 3; HCFP3

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

MEOX1; mesenchyme homeobox 1

3 of 14

600147

KLIPPEL-FEIL SYNDROME 2, AUTOSOMAL RECESSIVE; KFS2

Klippel-Feil syndrome 2

MEOX2; mesenchyme homeobox 2

3 of 14

600535

MNX1; motor neuron and pancreas homeobox 1

3 of 14

142994

CURRARINO SYNDROME

Currarino syndrome

PDX1; pancreatic and duodenal homeobox 1

3 of 14

600733

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0004054

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-48

Cytogenetic map

84A5-84A5

Sequence location

3R:6,743,135..6,744,136 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

84A5-84A5

Limits computationally determined from genome sequence between P{PZ}pb⁰⁴⁴⁹⁸ and P{lacW}l(3)L2100^L2100

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

84A1-84A1

(determined by in situ hybridisation)

(Rushlow et al., 1987)

84A-84B

(determined by in situ hybridisation)

(Rushlow et al., 1987)

Experimentally Determined Recombination Data

Location

3-48

(FlyBase, 2016)

3-44.2

(Comeron et al., 2012)

3-47.5

Left of (cM)

Right of (cM)

Notes

Map position inferred from close proximity to zen.

Stocks and Reagents

Stocks (9)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

94325

y¹ w^*; PBac{zen2-GFP.FPTB}VK00037

78272

y¹ sc^* v¹ sev²¹; P{TOE.GS02024}attP40

34649

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01124}attP2

FlyORF

F003402

M{UAS-zen2.ORF-CC}ZH-21F

F000689

M{UAS-zen2.ORF.3xHA.GW}ZH-86Fb

VDRC

v108130

P{KK102503}VIE-260B

v10406

w¹¹¹⁸; P{GD4144}v10406

v318130

PBac{fTRG00496.sfGFP-TVPTBF}VK00033

v341425

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD00490}attP40/CyO-GFP

Genomic Clones (32)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (14)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0004054

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0004054

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

The homologs of Antp, ftz, Scr, Dfd, Ama, bcd, zen, pb and lab, but not zen2 are all present in D.pseudoobscura.pseudoobscura, in the same linear order and similarly spaced along the chromosome as in D.melanogaster.

(Randazzo et al., 1993)

Comparative analysis of the homeobox sequences reveals the subdivision of the Antp-type homeobox genes into three classes early in metazoan evolution, one includes Abd-B, the second includes abd-A, Ubx, Antp, Scr, Dfd and ftz, and the third includes zen, zen2, pb and lab.

(Schubert et al., 1993)

No mutations corresponding to zen2 or deficiency exhibit a detectable phenotype so the function of zen2 is unknown.

(Kaufman et al., 1990)

A transient expression assay has been employed to investigate the potential of homeobox genes to function as transcriptional activators.

(Han et al., 1989)

Molecular and genetic analysis of the pb locus has been performed.

(Pultz et al., 1988)

Simultaneous deletion of both z2 and pb produces only a pb mutant phenotype. Thus absence of z2 function has no discernible effect on development or morphology.

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: zen2 CG1048

(Li, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

BG:DS00276.10

CG1048

(Hosono et al., 2015, Fukuoh et al., 2014, Ni et al., 2010.12.1, Keleman et al., 2009.8.5)

zpr

Name Synonyms

Zerknullt-related

(Cho et al., 2005)

zen-pattern-related

zerknullt 2

(Martin, 1999.1.14)

zerknullt-related

(Heimiller et al., 2014, Casillas et al., 2006, Negre et al., 2005)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 37 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40827

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P09090

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P09090

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q0IGT0

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P09090

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0004054

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40827/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004054

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004054

FlyMine - An integrated database for Drosophila genomics

FBgn0004054

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG1048

MARRVEL_MODEL - MARRVEL (model organism gene)

40827

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0004054

DroID - A comprehensive database of gene and protein interactions.

FBgn0004054

DRSC - Results frm RNAi screens

FBgn0004054

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

zen2_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004054

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0004054

Flygut - An atlas of the Drosophila adult midgut

FBgn0004054

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

References (90)

The following external sites may use different assemblies or annotations than FlyBase.

NA

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