FlyBase Gene Report: Dmel\aurB

General Information

Symbol

Dmel\aurB

Species

D. melanogaster

Name

aurora B

Annotation Symbol

CG6620

Feature Type

protein_coding_gene

FlyBase ID

FBgn0024227

Gene Model Status

Current

Stock Availability

14 publicly available

Enzyme Name (EC)

Gene Summary

aurora B (aurB) encodes a serine-threonine kinase and member of the chromosomal passenger complex. It plays multiple roles in mitosis including the correction of erroneous chromosome-spindle interactions, chromosome condensation, kinetochore assembly, spindle assembly checkpoint and cytokinesis. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

ial, Aurora B kinase, ABK, IplI-aurora-like kinase, DmAuroraB

Key Links

Genomic Location

Cytogenetic map

32B2-32B2

Sequence location

2L:10,856,797..10,858,094 [-]

Recombination map

2-43

RefSeq locus

NT_033779 REGION:10856797..10858094

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (27 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein serine/threonine kinase activity

inferred from mutant phenotype

(Giet and Glover, 2001)

inferred from direct assay

(Bell et al., 2015)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR017441

(InterPro Project Members, 2004-)

enables protein kinase activity

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR008271, InterPro:IPR030616

(InterPro Project Members, 2004-)

enables protein serine/threonine kinase activity

inferred from sequence or structural similarity with UniProtKB:Q96GD4

(Gene Ontology Curators, 2002-)

Biological Process (12 terms)

Terms Based on Experimental Evidence (8 terms)

CV Term

Evidence

References

involved_in chromatin organization

inferred from mutant phenotype

(Giet and Glover, 2001)

involved_in chromosome condensation

inferred from mutant phenotype

(Nikalayevich and Ohkura, 2015)

involved_in female germ-line stem cell asymmetric division

inferred from genetic interaction with FLYBASE:shrb; FB:FBgn0086656

(Matias et al., 2015)

involved_in midbody abscission

inferred from mutant phenotype

(Mathieu et al., 2013)

involved_in mitotic chromosome condensation

inferred from mutant phenotype

(Giet and Glover, 2001)

involved_in mitotic cytokinesis

inferred from mutant phenotype

(Giet and Glover, 2001)

inferred from genetic interaction with FLYBASE:shrb; FB:FBgn0086656

(Matias et al., 2015)

involved_in mitotic sister chromatid segregation

inferred from mutant phenotype

(Giet and Glover, 2001)

involved_in post-translational protein modification

inferred from mutant phenotype

(Giet and Glover, 2001)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in mitotic sister chromatid biorientation

inferred from sequence or structural similarity with UniProtKB:Q96GD4

(Gene Ontology Curators, 2002-)

involved_in mitotic spindle organization

inferred from biological aspect of ancestor with PANTHER:PTN000681967

(GO Reference Genome Project, 2011-)

involved_in regulation of cytokinesis

inferred from biological aspect of ancestor with PANTHER:PTN000681967

(GO Reference Genome Project, 2011-)

involved_in repair of mitotic kinetochore microtubule attachment defect

inferred from sequence or structural similarity with UniProtKB:Q96GD4

(Gene Ontology Curators, 2002-)

Cellular Component (12 terms)

Terms Based on Experimental Evidence (8 terms)

CV Term

Evidence

References

part_of chromosome passenger complex

inferred from direct assay

(Capalbo et al., 2012)

located_in chromosome, centromeric region

inferred from direct assay

(Orr and Sunkel, 2011, Giet and Glover, 2001)

located_in condensed chromosome, centromeric region

inferred from direct assay

(Delcros et al., 2006)

located_in kinetochore

inferred from direct assay

(Szafer-Glusman et al., 2011)

located_in meiotic spindle midzone

inferred from direct assay

(Szafer-Glusman et al., 2011)

located_in midbody

inferred from direct assay

(Capalbo et al., 2012, Giet and Glover, 2001)

located_in spindle midzone

inferred from direct assay

(Jang et al., 2005)

located_in spindle

inferred from direct assay

(Giet and Glover, 2001)

Terms Based on Predictions or Assertions (7 terms)

CV Term

Evidence

References

is_active_in centrosome

inferred from biological aspect of ancestor with PANTHER:PTN001219008

(GO Reference Genome Project, 2011-)

part_of chromosome passenger complex

inferred from biological aspect of ancestor with PANTHER:PTN000681967

(GO Reference Genome Project, 2011-)

is_active_in kinetochore

inferred from biological aspect of ancestor with PANTHER:PTN000681968

(GO Reference Genome Project, 2011-)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000681967

(GO Reference Genome Project, 2011-)

is_active_in spindle microtubule

inferred from biological aspect of ancestor with PANTHER:PTN000681967

(GO Reference Genome Project, 2011-)

is_active_in spindle midzone

inferred from biological aspect of ancestor with PANTHER:PTN000681967

(GO Reference Genome Project, 2011-)

is_active_in spindle pole

inferred from biological aspect of ancestor with PANTHER:PTN000681968

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

UNCLASSIFIED CONVENTIONAL PROTEIN KINASE DOMAIN KINASES

MITOTOIC CHROMOSOMAL PASSENGER COMPLEX

MEIOTIC CHROMOSOMAL PASSENGER COMPLEX

Protein Family (UniProt)

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily. (Q9VKN7)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

protein serine/threonine kinase activity

Summaries

Gene Snapshot

Gene Group (FlyBase)

UNCLASSIFIED CONVENTIONAL PROTEIN KINASE DOMAIN KINASES -: This group comprises conventional protein kinase domain kinases that do not classify under other groups in FlyBase.
MITOTOIC CHROMOSOMAL PASSENGER COMPLEX -: The Mitotic Chromosomal Passenger Complex plays several key roles in mitosis including kinetochore-microtubule attachment, spindle assembly and cytokinesis. (Adapted from FBrf0216422 and FBrf0202888).
MEIOTIC CHROMOSOMAL PASSENGER COMPLEX -: The Meiotic Chromosomal Passenger Complex plays several key roles in meiosis including kinetochore-microtubule attachment, spindle assembly and cytokinesis. (Adapted from FBrf0216422 and FBrf0202888).

Protein Function (UniProtKB)

Serine/threonine-protein kinase that mediates both meiotic and mitotic chromosome segregation. Required for histone H3 'Ser-10' phosphorylation. Phosphorylates mei-S332 within residues 124-126 and stabilizes its association with centromeres during meiosis. May regulate the function of the ESCRT-III complex core component shrb during abscission of germline cells in oogenesis (PubMed:25647097).

(UniProt, Q9VKN7)

Summary (Interactive Fly)

signaling protein involved in chromatin condensation during mitosis

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\aurB for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VKN7)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.45

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

aurB-RA

FBtr0080180

NM_057988

1183

329

Additional Transcript Data and Comments

Reported size (kB)

1.17 (longest cDNA)

(Mesilaty-Gross et al., 1999)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

aurB-PA

FBpp0079769

38.3

329

8.98

Q9VKN7

NP_477336

AAF53026

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

329 (aa)

(Mesilaty-Gross et al., 1999)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with Incenp and Cdc37.

(UniProt, Q9VKN7)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VKN7

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\aurB using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

aurB_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

2.31

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4

organism | ubiquitous | uniform

(Mesilaty-Gross et al., 1999)

embryonic stage 4 -- 6

organism | ubiquitous

• cytoplasm | basal

Comment: apically cleared

(Fisher et al., 2012)

embryonic stage 7 -- 8

antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

visual anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

central brain primordium

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 11 -- 13

larval ventral nerve cord | segmentally repeated

(Mesilaty-Gross et al., 1999)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

larval nervous system

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

adult stage | female oogenesis stage S9 -- S14

nurse cell

• cytoplasm

(Mesilaty-Gross et al., 1999)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Mesilaty-Gross et al., 1999)

embryonic stage 4

(Mesilaty-Gross et al., 1999)

embryonic stage 7 -- 17

organism | segmentally repeated

(Mesilaty-Gross et al., 1999)

embryonic stage 14 -- 17

larval ventral nerve cord | restricted

(Mesilaty-Gross et al., 1999)

adult stage & oogenesis

oocyte

(Mesilaty-Gross et al., 1999)

adult stage oogenesis stage S9 -- S10

nurse cell

(Mesilaty-Gross et al., 1999)

adult stage oogenesis stage S9 -- S14

(Mesilaty-Gross et al., 1999)

Additional Descriptive Data

Stam and aurB transcripts are detected in all developmental stages, but are at highest levels in embryos and adults. Stam and aurB transcripts are detected at very high levels in syncytial blastoderm embryos. From stage 7 onwards, a segmentally repeated pattern is visible for both transcripts. Expression is detected in the developing ventral nerve cord; later, a limited number of cells in the condensed nerve cord continue to express Stam and aurB transcripts. Stam and aurB transcript expression is also detected during oogenesis, starting at stage S9. In stages S9 and S10, expression is high in the nurse cell cytoplasm. Transcript is detected in deposited eggs.

(Mesilaty-Gross et al., 1999)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage & oogenesis

ovariole

(Mesilaty-Gross et al., 1999)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of chromosome passenger complex

inferred from direct assay

(Capalbo et al., 2012)

located_in chromosome, centromeric region

inferred from direct assay

(Orr and Sunkel, 2011, Giet and Glover, 2001)

located_in condensed chromosome, centromeric region

inferred from direct assay

(Delcros et al., 2006)

located_in kinetochore

inferred from direct assay

(Szafer-Glusman et al., 2011)

located_in meiotic spindle midzone

inferred from direct assay

(Szafer-Glusman et al., 2011)

located_in midbody

inferred from direct assay

(Capalbo et al., 2012, Giet and Glover, 2001)

located_in spindle midzone

inferred from direct assay

(Jang et al., 2005)

located_in spindle

inferred from direct assay

(Giet and Glover, 2001)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\aurB in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\aurB

View exonic expression by tissue for Dmel\aurB

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0024227

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34504/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0024227

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0024227

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0024227

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6620

Flygut - An atlas of the Drosophila adult midgut

FBgn0024227

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\aurB

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 7 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 23 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of aurB

Transgenic constructs containing regulatory region of aurB

Aberrations (Deficiencies and Duplications) ( 6 )

Inferred from experimentation ( 6 )

Gene disrupted in

Df(2L)1469

(Chang et al., 2006)

Df(2L)BSC213

(Taniguchi et al., 2018)

Df(2L)BSC214

(Taniguchi et al., 2018)

Df(2L)Exel7049

(Ryder, 2004.4.26)

Df(2L)Exel8026

(Taniguchi et al., 2018, Ryder, 2004.4.26)

Df(2L)J39

(Wides et al., 1998)

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

lethal, with Scer\GAL4^insc-Mz1407

aurB^GD11982

lethal, with Scer\GAL4^αTub84B.PL

aurB^GL00202

lethal - all die before end of first instar larval stage | recessive

aurB^L27

partially lethal - majority die, with Scer\GAL4^pnr-MD237

aurB^GD11982

viable

aurB¹⁶⁸⁹

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

aurB^GD11982

viable, with Scer\GAL4^tin.CΔ4

aurB^GD11982

Sterility

Allele

female sterile, with Scer\GAL4^{VP16.nanos.UTR}

aurB^GL00202

female sterile, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

aurB^GL00202

Other Phenotypes

Allele

abnormal cell cycle

aurB¹⁶⁸⁹

abnormal meiotic cell cycle | oogenesis

aurB¹⁶⁸⁹

abnormal meiotic cell cycle | oogenesis, with Scer\GAL4^{VP16.mat.αTub67C}

aurB^GL00202

abnormal mitotic cell cycle | adult stage | somatic clone

aurB^L27

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

aurB^GD11982

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

aurB^GD11982

abnormal size, with Scer\GAL4^sd.PU

aurB^G213D.UAS

abnormal size | adult stage, with Scer\GAL4^NP5399

aurB^G213D.UAS

decreased cell number | adult stage, with Scer\GAL4^NP5399

aurB^G213D.UAS

decreased cell number | adult stage | recessive | somatic clone

aurB^L27

decreased cell number | larval stage, with Scer\GAL4^Hml.Δ

aurB^JF03107

decreased cell number | larval stage | recessive | somatic clone

aurB^L27

decreased cell number | larval stage | somatic clone, with Scer\GAL4^Act5C.PI

aurB^G213D.UAS

decreased occurrence of cell division | adult stage | recessive | somatic clone

aurB^L27

decreased occurrence of cell division | larval stage | recessive | somatic clone

aurB^L27

decreased occurrence of cell division | larval stage | somatic clone, with Scer\GAL4^Act5C.PI

aurB^G213D.UAS

decreased occurrence of cell division | third instar larval stage (with aurB^2A43)

aurB¹⁶⁸⁹

decreased occurrence of cell division | third instar larval stage (with aurB^35.33)

aurB¹⁶⁸⁹

decreased occurrence of cell division | third instar larval stage (with aurB¹⁶⁸⁹)

hypoplasia, with Scer\GAL4^mirr-DE

increased cell number | adult stage | female, with Scer\GAL4^{VP16.nanos.UTR}

aurB^UASp.Tag:HA

increased cell size | adult stage | recessive | somatic clone

aurB^L27

increased cell size | larval stage | recessive | somatic clone

aurB^L27

increased occurrence of cell division | embryonic stage, with Scer\GAL4^Mef2.PR

aurB^{UASp.Tag:proteinA}

some die during pupal stage, with Scer\GAL4^pnr-MD237

aurB^GD11982

visible, with Scer\GAL4^pnr-MD237

aurB^GD11982

Phenotype manifest in

Allele

chromosome | adult stage | female, with Scer\GAL4^{VP16.mat.αTub67C}

aurB^GL00202

egg chamber

aurB¹⁶⁸⁹

egg chamber, with Scer\GAL4^{VP16.nanos.UTR}

aurB^UASp.Tag:HA

embryonic/larval crystal cell | decreased number | larval stage, with Scer\GAL4^Hml.Δ

aurB^JF03107

eye disc | larval stage, with Scer\GAL4^mirr-DE

female fusome

aurB¹⁶⁸⁹

female germline cyst

aurB¹⁶⁸⁹

female germline cyst, with Scer\GAL4^{VP16.nanos.UTR}

aurB^GL00202

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

aurB^GD11982

germarium | germline clone

germline cell

aurB¹⁶⁸⁹

germline cell | germline clone

intestinal stem cell | adult stage, with Scer\GAL4^esg.PU, Scer\GAL80^Su(H).GBE

aurB^KK112558

kinetochore, with Scer\GAL4^{VP16.mat.αTub67C}

aurB^GL00202

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

aurB^GD11982

larval neuroblast | third instar larval stage (with aurB^2A43)

aurB¹⁶⁸⁹

larval neuroblast | third instar larval stage (with aurB^35.33)

aurB¹⁶⁸⁹

larval neuroblast | third instar larval stage (with aurB¹⁶⁸⁹)

male accessory gland | adult stage, with Scer\GAL4^NP5399

aurB^G213D.UAS

male accessory gland | adult stage | somatic clone

aurB^L27

mesoderm | embryonic stage, with Scer\GAL4^Mef2.PR

aurB^{UASp.Tag:proteinA}

mesonotum, with Scer\GAL4^pnr-MD237

aurB^GD11982

microtubule, with Scer\GAL4^{VP16.mat.αTub67C}

aurB^GL00202

microtubule | adult stage | female, with Scer\GAL4^{VP16.mat.αTub67C}

aurB^GL00202

mitotic spindle | adult stage | somatic clone

aurB^L27

muscle cell of ventral longitudinal muscle 1 | embryonic stage, with Scer\GAL4^Mef2.PR

aurB^{UASp.Tag:proteinA}

nucleus | adult stage, with Scer\GAL4^esg.PU, Scer\GAL80^Su(H).GBE

aurB^KK112558

nucleus | adult stage, with Scer\GAL4^NP5399

aurB^G213D.UAS

nucleus | adult stage | somatic clone

aurB^L27

nucleus | embryonic stage | increased number, with Scer\GAL4^Mef2.PR

aurB^{UASp.Tag:proteinA}

nucleus | germline clone

nucleus | larval stage | somatic clone

aurB^L27

nucleus | somatic clone, with Scer\GAL4^Act5C.PI

aurB^G213D.UAS

nurse cell

aurB¹⁶⁸⁹

nurse cell, with Scer\GAL4^{VP16.nanos.UTR}

aurB^UASp.Tag:HA

nurse cell spectrosome | germline clone

oocyte

aurB¹⁶⁸⁹

oocyte, with Scer\GAL4^{VP16.mat.αTub67C}

aurB^GL00202

oocyte, with Scer\GAL4^{VP16.nanos.UTR}

aurB^UASp.Tag:HA

ring canal

aurB¹⁶⁸⁹

ring canal, with Scer\GAL4^{VP16.nanos.UTR}

aurB^UASp.Tag:HA

spindle microtubule | oogenesis, with Scer\GAL4^{VP16.mat.αTub67C}

aurB^GL00202

wing, with Scer\GAL4^sd.PU

aurB^G213D.UAS

wing disc | somatic clone

aurB^L27

wing disc | somatic clone, with Scer\GAL4^Act5C.PI

aurB^G213D.UAS

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (36)

Hsap\AURKA

7 of 14

Yes

Hsap\AURKB

7 of 14

Yes

Hsap\AURKC

7 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Hsap\SIK1B

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (19)

Rnor\Aurka

7 of 14

Yes

Rnor\Aurkb

7 of 14

Yes

Rnor\Aurkc

7 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (21)

Mmus\Aurkc

8 of 14

Yes

Mmus\Aurkb

7 of 14

Mmus\Aurka

6 of 14

Mmus\Gm7207

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (25)

Xtro\aurka

5 of 13

Yes

Xtro\aurkb

5 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\snrk

1 of 13

Xtro\ulk1

1 of 13

Xtro\ulk3

1 of 13

Danio rerio (Zebrafish) (25)

Drer\aurka

7 of 14

Yes

Drer\aurkb

7 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:ch211-195b13.1

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (13)

Cele\air-2

10 of 14

Yes

7 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (10)

Agam\AgaP_AGAP007855

12 of 12

Yes

Agam\AgaP_AGAP001793

6 of 12

Agam\AgaP_AGAP012456

3 of 12

Yes

Agam\AgaP_AGAP012876

3 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (36)

Atha\AUR3

8 of 13

Yes

7 of 13

7 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (7)

Scer\IPL1

8 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (5)

Spom\ark1

6 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:aurB. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (12)

10 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

SAK

1 of 13

Sik2

1 of 13

Sik3

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 2 )

Human Ortholog

Disease

Evidence

References

AURKA; aurora kinase A

model of colorectal cancer

IEA

(FlyBase, 2019-)

AURKC; aurora kinase C

model of spermatogenic failure 5

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

aurB^JF03107

ameliorates pancreatic ductal adenocarcinoma

modeled by CycE^{UAS.Tag:HA.Medsh}, Med^{RNAi.UAS.CycE-HA}, p53^{RNAi.UAS.RasG12D}

(Sekiya et al., 2023)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

AURKA; aurora kinase A

7 of 14

603072

COLORECTAL CANCER; CRC

colorectal cancer

AURKB; aurora kinase B

7 of 14

604970

AURKC; aurora kinase C

7 of 14

603495

SPERMATOGENIC FAILURE 5; SPGF5

spermatogenic failure 5

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

aurB - Incenp

anti tag coimmunoprecipitation, anti tag western blot, peptide massfingerprinting, proximity ligation assay, fluorescence microscopy, pull down, autoradiography

(Carmena et al., 2012, D'Avino et al., 2009, Resnick et al., 2006)

aurB - borr

anti tag coimmunoprecipitation, peptide massfingerprinting

(D'Avino et al., 2009)

aurB - polo

enzymatic study, autoradiography

(Carmena et al., 2012)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

aurB

suppressible

bam

(Mathieu et al., 2013)

aurB

suppressible

CycB

(Mathieu et al., 2013)

aurB

suppressible

polo

(Gallaud et al., 2022)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

aurA

enhanceable

aurB

(Gallaud et al., 2022)

Det

suppressible

aurB

(Mathieu et al., 2013)

pal

suppressible

aurB

(Dubruille et al., 2023)

Ras85D|p53|CycE|Med

suppressible

aurB

(Sekiya et al., 2023)

shrb

suppressible

aurB

(Matias et al., 2015)

External Data

Subunit Structure (UniProtKB)

Interacts with Incenp and Cdc37.

(UniProt, Q9VKN7 )

Linkouts

BioGRID - A database of protein and genetic interactions.

60576

DroID - A comprehensive database of gene and protein interactions.

FBgn0024227

MIST (protein-protein) - An integrated Molecular Interaction Database

34504

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Polycomb repressive complex

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9VKN7

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-43

Cytogenetic map

32B2-32B2

Sequence location

2L:10,856,797..10,858,094 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

32B2-32B2

Limits computationally determined from genome sequence between P{PZ}dbf¹ and P{lacW}SCAR^k03107

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

32B-32B

(Reich, 1999.1.21, Wides et al., 1998)

Location determined by physical mapping.

(Wides et al., 1998)

Experimentally Determined Recombination Data

Location

2-43

(FlyBase, 2016)

2-36.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (14)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

28691

y¹ v¹; P{TRiP.JF03107}attP2

58308

y¹ v¹; P{TRiP.HMJ22415}attP40/CyO

85301

w^*; P{UASp-aurB.PrA}3/TM6C, Sb¹ Tb¹

35299

y¹ sc^* v¹ sev²¹; P{TRiP.GL00202}attP2

FlyORF

F001345

M{UAS-aurB.ORF}ZH-86Fb

F001197

M{UAS-aurB.ORF.3xHA}ZH-86Fb

NIG-Fly

6620R-2

P{NIG.6620R}2

6620R-3

P{NIG.6620R}3

HMJ22415

y¹ v¹; P{TRiP.HMJ22415}attP40/CyO

VDRC

v104051

P{KK112558}VIE-260B

v35107

w¹¹¹⁸ P{GD11982}v35107

v342000

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD01348}attP40/CyO-GFP

More stocks available...

Genomic Clones (11)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (29)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD39409

Other clones

DPiM_LD39409

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0024227

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0024227

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

New stable cell line derived from S2-unspecified : S2 cell lines expressing His2B GFP/mCherry-alpha-tubulin or GFP-aurB/mCherry-alpha-tubulin were created.
(Afonso et al., 2014)
New stable cell line derived from S2-unspecified : S2 cells stably expressing ken-CycB-GFP/mCherry-alpha-tubulin or GFP-aurB/WTCycB-mCherry were created.
(Afonso et al., 2019)

Other Comments

aurB delays abscission by antagonising CycB activity in germline cells.

(Mathieu et al., 2013)

DNA-protein interactions: genome-wide binding profile assayed for ial protein in Kc167 cells; see Chromatin_types_NKI collection report. Individual protein-binding experiments listed under "Samples" at GEO_GSE22069 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE22069).

(Filion et al., 2010)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes defects in spindle shape when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, a decrease in cytokinetic index, centrosome abnormalities, a whole range of mitotic abnormalities, chromosome abnormalities are seen.

(Bettencourt-Dias et al., 2004)

dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells.

(Echard et al., 2004)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a binucleation phenotype when assayed in Kc₁₆₇ cells.

(Eggert et al., 2004)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R⁺ cells: binucleate cells.

(Kiger et al., 2003)

Depleting ial using RNAi in cultured cells results in polyploidy.

(Giet and Glover, 2001)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: ial aurB

(FlyBase, 2009-)

Nomenclature comments

'ial' renamed to 'aurB' to reflect preferred usage in the literature and to match the related 'aurA' gene.

(FlyBase, 2009-)

Etymology

Synonyms and Secondary IDs (27)

Reported As

Symbol Synonym

ABK

(Audett et al., 2022, Verma and Maresca, 2019, Salemi et al., 2013)

AURKB

(Mitchison and Field, 2021)

Aur

(Lou et al., 2017)

Aur B

(Szafer-Glusman et al., 2011)

AurB

(Khalili et al., 2023, Nagai et al., 2023, Almeida Machado Costa et al., 2022, Repton et al., 2022, Llorens-Giralt et al., 2021, Oliveira et al., 2019, Park et al., 2019, Roubinet et al., 2017, Bell et al., 2015, Cane et al., 2013, Cane et al., 2013, Cui et al., 2013, Debruhl et al., 2013, Crest et al., 2012, Jang et al., 2005, Zavortink et al., 2005, Terada et al., 2003)

Aurora B

(Aguilar-Aragon et al., 2020, Capalbo et al., 2019, Silva et al., 2018, Beaven et al., 2017, Karg et al., 2015, Yung et al., 2015, Noatynska et al., 2013, Carmena et al., 2012, Giansanti et al., 2012, McKee et al., 2012, Colombié et al., 2008)

Aurora-B

(Gilliland et al., 2007)

CG6620

(National Institute of Genetics Fly Stocks, 2022-, Ibaraki et al., 2021, Nikalayevich and Ohkura, 2015, Fukuoh et al., 2014, Cui et al., 2013, Moutinho-Pereira et al., 2013, Schertel et al., 2013, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Goshima et al., 2007, Morrison et al., 2000)

DmAIRK2

(Giet and Prigent, 1999)

DmAurB

(Adams et al., 2001)

DmAuroraB

(Adams et al., 2001)

IAL

(Zhimulev et al., 2024, Parey and Crombach, 2019, Filion et al., 2010, Giet and Prigent, 1999, Mesilaty-Gross, 1999.1.20)

IAL1

(Bischoff and Plowman, 1999)

Ial

(Antao et al., 2012, Somma et al., 2008, Goshima et al., 2007, De Lucia et al., 2005)

aurora B

(Bettencourt-Dias et al., 2004)

ial/AurB

(Yin et al., 2013)

Name Synonyms

AURORA-like

(Morrison et al., 2000)

Aurora

(Coelho et al., 2008)

Aurora B

(Warecki et al., 2023, Saha and Mishra, 2019, Bell et al., 2015, Maiato et al., 2015, Nikalayevich and Ohkura, 2015, Afonso et al., 2014, Carmena et al., 2014, Liang et al., 2014, Nogueira et al., 2014, Cane et al., 2013, Conde et al., 2013, Mathieu et al., 2013, Moutinho-Pereira et al., 2013, Capalbo et al., 2012, Crest et al., 2012, Giansanti and Fuller, 2012, Chen et al., 2011, Szafer-Glusman et al., 2011, Oliveira et al., 2010, Bucciarelli et al., 2009, D'Avino et al., 2009, Hughes et al., 2009, Kondo et al., 2009, McCleland et al., 2009, Vale et al., 2009, Archambault et al., 2008, Gao et al., 2008, Somma et al., 2008, Brittle et al., 2007, Jang et al., 2007, Cesario et al., 2006, Chang et al., 2006, Delcros et al., 2006, Jang et al., 2006, Resnick et al., 2006, Resnick et al., 2006, Anonymous, 2005, D'Avino et al., 2005, De Lucia et al., 2005, Maia and Sunkel, 2005, Redding et al., 2005, Zavortink et al., 2005, Balasubramanian et al., 2004, Bucciarelli et al., 2002, Lange et al., 2002, Shannon and Salmon, 2002)

Aurora B kinase

(Mehrotra and Deshpande, 2018, Warecki and Sullivan, 2018, Nogueira et al., 2014, Oliveira et al., 2010, Vale et al., 2009, Perrimon and Mathey-Prevot, 2007, Pines, 2006, Zavortink et al., 2005)

Aurora-B

(Hinnant et al., 2020, Royou et al., 2010, Gilliland et al., 2007, Mishima and Glotzer, 2003)

AuroraB

(Spratford et al., 2021, Kitazawa et al., 2014, Hickson et al., 2003, Lee and Orr-Weaver, 2003, Parry et al., 2003)

DmAuroraB

(Chang et al., 2003)

IplI-aurora-like kinase

(Mathieu et al., 2013, Wides et al., 1998)

aurora B

(Lee et al., 2019, Cesario and McKim, 2008, Prigent et al., 2005, Hirano, 2002)

aurora B kinase

(Salemi et al., 2013, Anonymous, 2003, Uhlmann, 2001)

aurora-B

(Earnshaw and Carmena, 2003, Adams et al., 2001)

auroraB

(Lesch and Page, 2012, De Lucia et al., 2005, Giet and Glover, 2001)

dAurora B

(Hanson and Bienz, 2005)

Secondary FlyBase IDs

Datasets (1)

Study focus (1)

Experimental Role

Project

Project Type

Title

transgene_used

FBGSE22069

genome binding

Protein profiling reveals five principal chromatin types in Drosophila cells.

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 46 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34504

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VKN7

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VKN7

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VKN7

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0024227

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34504/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0024227

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0024227

FlyMine - An integrated database for Drosophila genomics

FBgn0024227

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG6620

MARRVEL_MODEL - MARRVEL (model organism gene)

34504

Linkouts

BioGRID - A database of protein and genetic interactions.

60576

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0024227

DroID - A comprehensive database of gene and protein interactions.

FBgn0024227

DRSC - Results frm RNAi screens

FBgn0024227

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

aurB_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0024227

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0024227

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6620

Flygut - An atlas of the Drosophila adult midgut

FBgn0024227

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0024227

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC001943

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Polycomb repressive complex

MIST (protein-protein) - An integrated Molecular Interaction Database

34504

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9VKN7

References (241)

The following external sites may use different assemblies or annotations than FlyBase.

2.31

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