FlyBase Gene Report: Dmel\Rab35

General Information

Symbol

Dmel\Rab35

Species

D. melanogaster

Name

Rab35

Annotation Symbol

CG9575

Feature Type

protein_coding_gene

FlyBase ID

FBgn0031090

Gene Model Status

Current

Stock Availability

16 publicly available

Gene Summary

Rab35 (Rab35) encodes a protein that regulates proximal versus distal growth of seamless tubes in the terminal cells of the tracheal system. [Date last reviewed: 2018-10-04] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

DmRab35, dRab35

Key Links

Genomic Location

Cytogenetic map

19B3-19B3

Sequence location

X:20,155,766..20,159,872 [-]

Recombination map

1-64

RefSeq locus

NC_004354 REGION:20155766..20159872

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (34 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables GTP binding

inferred from biological aspect of ancestor with PANTHER:PTN002785905

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR001806, InterPro:IPR005225

(InterPro Project Members, 2004-)

enables GTPase activity

inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN002785905

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR001806

(InterPro Project Members, 2004-)

inferred from electronic annotation with InterPro:IPR001806, InterPro:IPR041815

(InterPro Project Members, 2004-)

Biological Process (19 terms)

Terms Based on Experimental Evidence (16 terms)

CV Term

Evidence

References

involved_in apical protein localization

inferred from mutant phenotype

(Jewett et al., 2017)

involved_in cytoskeletal rearrangement involved in phagocytosis, engulfment

inferred from mutant phenotype

(Shim et al., 2010)

involved_in endosome organization

inferred from mutant phenotype

(Carim et al., 2019)

involved_in endosome to plasma membrane protein transport

inferred from mutant phenotype

(Shim et al., 2010)

involved_in exosomal secretion

inferred from mutant phenotype

(Tsarouhas et al., 2023, Corrigan et al., 2014)

acts_upstream_of liquid clearance, open tracheal system

inferred from mutant phenotype

(Tsarouhas et al., 2023)

involved_in lumen formation, open tracheal system

inferred from mutant phenotype

(Schottenfeld-Roames and Ghabrial, 2012)

involved_in mitotic cytokinesis

inferred from mutant phenotype

(Kouranti et al., 2006)

involved_in plasma membrane to endosome transport

inferred from mutant phenotype

(Jewett et al., 2017)

involved_in polarity specification of proximal/distal axis

inferred from mutant phenotype

(Schottenfeld-Roames and Ghabrial, 2012)

involved_in positive regulation of apical constriction involved in ventral furrow formation

inferred from mutant phenotype

(Jewett et al., 2017)

involved_in positive regulation of establishment of protein localization

inferred from mutant phenotype

(Jewett et al., 2017)

involved_in positive regulation of filopodium assembly

inferred from mutant phenotype

(Shim et al., 2010)

involved_in regulation of embryonic cell shape

inferred from mutant phenotype

(Jewett et al., 2017)

involved_in symbiont entry into host cell

inferred from high throughput mutant phenotype

(Hodgson et al., 2019)

involved_in synaptic vesicle endosomal processing

inferred from mutant phenotype

(Uytterhoeven et al., 2011)

inferred from direct assay

(Uytterhoeven et al., 2011)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in endocytic recycling

inferred from biological aspect of ancestor with PANTHER:PTN001178320

(GO Reference Genome Project, 2011-)

involved_in Rab protein signal transduction

inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790

(Gene Ontology Curators, 2002-)

inferred from electronic annotation with InterPro:IPR041815

(InterPro Project Members, 2004-)

involved_in vesicle-mediated transport

inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790

(Gene Ontology Curators, 2002-)

Cellular Component (13 terms)

Terms Based on Experimental Evidence (11 terms)

CV Term

Evidence

References

located_in anterior cell cortex

inferred from direct assay

(Jewett et al., 2017)

located_in cytoplasmic side of apical plasma membrane

inferred from direct assay

(Schottenfeld-Roames and Ghabrial, 2012)

located_in cytoplasm

inferred from direct assay

(Schottenfeld-Roames and Ghabrial, 2012)

located_in endosome to plasma membrane transport vesicle

inferred from direct assay

(Shim et al., 2010)

located_in filopodium

inferred from direct assay

(Schottenfeld-Roames and Ghabrial, 2012)

located_in lamellipodium

inferred from direct assay

(Shim et al., 2010)

is_active_in neuromuscular junction

inferred from mutant phenotype

(Uytterhoeven et al., 2011)

inferred from direct assay

(Uytterhoeven et al., 2011)

located_in neuronal cell body

inferred from high throughput direct assay

(Chan et al., 2011)

located_in plasma membrane

inferred from high throughput direct assay

(Tan et al., 2009)

located_in posterior cell cortex

inferred from direct assay

(Jewett et al., 2017)

located_in synapse

inferred from high throughput direct assay

(Chan et al., 2011)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

is_active_in endosome membrane

inferred from biological aspect of ancestor with PANTHER:PTN001178320

(GO Reference Genome Project, 2011-)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN001178320

(GO Reference Genome Project, 2011-)

located_in vesicle

inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790

(Gene Ontology Curators, 2002-)

Gene Group (FlyBase)

RAB GTPASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

GTPase activity
RHEA 19669:

Summaries

Gene Snapshot

Rab35 (Rab35) encodes a protein that regulates proximal versus distal growth of seamless tubes in the terminal cells of the tracheal system. [Date last reviewed: 2018-10-04]

Gene Group (FlyBase)

RAB GTPASES -: The Rab family are members of the Ras superfamily of small GTPases. Rabs regulate vesicle trafficking including cargo selection, vesicle budding, transport, docking and targeting. They localize to different intracellular compartments directed by specific isoprenylation of C-terminal motifs. (Adapted from PMID:15731001).

Summary (Interactive Fly)

tissue polarity, an essential component of the contractile process that functions as a membrane ratchet to ensure unidirectional movement of intercalating cells - gastrulation - mesoderm invagination - Whacked and Rab35 polarize dynein-motor-complex-dependent seamless tube growth during tracheal development - Skywalker facilitates endosomal trafficking of synaptic vesicles at NMJ boutons by controlling Rab35 activity

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Rab35 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9W5X0)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Rab35-RA

FBtr0070028

NM_134529

2635

201

Rab35-RB

FBtr0303258

NM_001201749

2024

201

Rab35-RC

FBtr0333548

NM_001272809

2159

190

Rab35-RD

FBtr0333549

NM_001272810

3348

201

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Rab35-PA

FBpp0070027

22.9

201

8.82

FBpp0292350

22.9

201

8.82

FBpp0305728

21.6

190

8.62

FBpp0305729

22.9

201

8.82

Q9W5X0

NP_001259739

AGB95578

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

201 aa isoforms: Rab35-PA, Rab35-PB, Rab35-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rab35 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Rab35_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.66

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in anterior cell cortex

inferred from direct assay

(Jewett et al., 2017)

located_in cytoplasmic side of apical plasma membrane

inferred from direct assay

(Schottenfeld-Roames and Ghabrial, 2012)

located_in cytoplasm

inferred from direct assay

(Schottenfeld-Roames and Ghabrial, 2012)

located_in endosome to plasma membrane transport vesicle

inferred from direct assay

(Shim et al., 2010)

located_in filopodium

inferred from direct assay

(Schottenfeld-Roames and Ghabrial, 2012)

located_in lamellipodium

inferred from direct assay

(Shim et al., 2010)

is_active_in neuromuscular junction

inferred from mutant phenotype

(Uytterhoeven et al., 2011)

inferred from direct assay

(Uytterhoeven et al., 2011)

located_in neuronal cell body

inferred from high throughput direct assay

(Chan et al., 2011)

located_in plasma membrane

inferred from high throughput direct assay

(Tan et al., 2009)

located_in posterior cell cortex

inferred from direct assay

(Jewett et al., 2017)

located_in synapse

inferred from high throughput direct assay

(Chan et al., 2011)

Expression Deduced from Reporters

Reporter: PBac{I-SceI(FRT.Rab35-GAL4.ATG(loxP.3xP3-RFP))}

Stage

Tissue/Position (including subcellular localization)

Reference

pupal stage P6 -- P7

neuron of pupal brain

Comment: 30-40h APF

(Chan et al., 2011)

mushroom body

Comment: 30-40h APF

(Chan et al., 2011)

neuropil glial sheath

Comment: 30-40h APF

(Jin et al., 2012)

glial cell of second optic chiasma | intense

Comment: 30-40h APF

(Jin et al., 2012)

neuron of adult optic lobe

Comment: 30-40h APF

(Jin et al., 2012)

mushroom body | intense

Comment: 30-40h APF

(Jin et al., 2012)

eye photoreceptor cell

Comment: 30-40h APF

(Jin et al., 2012)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Rab35 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Rab35

View exonic expression by tissue for Dmel\Rab35

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0031090

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

33014/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0031090

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0031090

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0031090

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9575

Flygut - An atlas of the Drosophila adult midgut

FBgn0031090

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 15 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Rab35

Transgenic constructs containing regulatory region of Rab35

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene partially disrupted in

Df(1)Phf7^Δ88

(Yang et al., 2012)

Inferred from location ( 14 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal

Rab35^CRISPR

partially lethal - majority die, with Scer\GAL4^Rab35.ATG

Rab35^S22N.UAS.YFP

viable

Rab35^EYFP

Other Phenotypes

Allele

abnormal neuroanatomy | third instar larval stage, with Hsim\VP16^AD.nrv2, Scer\GAL4^{DBD.wrapper.932i}

abnormal neurophysiology, with Scer\GAL4^nSyb.PS

abnormal planar polarity, with Avic\GFP^RNAi.R.UAS, Scer\GAL4^nub.PK

Rab35^EYFP

abnormal planar polarity, with Avic\GFP^{RNAi.UAS.V20.3}, Scer\GAL4^nub.PK

Rab35^EYFP

Phenotype manifest in

Allele

dorsal tracheal branch primordium

Rab35^CRISPR

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, Scer\GAL4^{DBD.wrapper.932i}

embryonic/larval neuromuscular junction, with Scer\GAL4^nSyb.PS

extracellular exosome, with Scer\GAL4^dsx.KI

Rab35^JF02978

larval tracheal system | embryonic stage, with Scer\GAL4^btl.PS

Rab35^S22N.UAS.YFP

male accessory gland secondary cell, with Scer\GAL4^dsx.KI

Rab35^JF02978

tracheal lumen | embryonic stage, with Scer\GAL4^btl.PS

Rab35^S22N.UAS.YFP

wing hair, with Avic\GFP^RNAi.R.UAS, Scer\GAL4^nub.PK

Rab35^EYFP

wing hair, with Avic\GFP^{RNAi.UAS.V20.3}, Scer\GAL4^nub.PK

Rab35^EYFP

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (92)

Hsap\RAB35

11 of 14

Yes

Hsap\IFT27

2 of 14

Yes

Hsap\RAB1A

2 of 14

Hsap\RAB1B

2 of 14

Hsap\RAB12

2 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

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Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (60)

Rnor\Rab35

12 of 14

Yes

Rnor\Ift27

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

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1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (60)

Mmus\Rab35

11 of 14

Yes

Mmus\Ift27

2 of 14

Yes

Mmus\Rab1a

2 of 14

Mmus\Rab1b

2 of 14

Mmus\Rab12

2 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

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1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (53)

Xtro\rab35l

9 of 13

Yes

Xtro\rab35

8 of 13

Yes

Xtro\rab1a

2 of 13

Xtro\rab12

2 of 13

Yes

Xtro\rab19

2 of 13

Xtro\arl8a

1 of 13

Xtro\cplane2

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\ift27

1 of 13

Yes

Xtro\LOC100488596

1 of 13

Xtro\LOC100496232

1 of 13

Yes

Xtro\MGC147600

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

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Yes

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Yes

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Yes

1 of 13

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1 of 13

Danio rerio (Zebrafish) (76)

Drer\rab35b

12 of 14

Yes

Drer\rab35a

9 of 14

Yes

Drer\si:dkey-16l2.16

8 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\ift27

1 of 14

Yes

1 of 14

1 of 14

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1 of 14

Caenorhabditis elegans (Nematode, roundworm) (41)

Cele\rab-35

13 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

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1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (37)

Agam\AgaP_AGAP003028

11 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

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1 of 12

Arabidopsis thaliana (thale-cress) (61)

Atha\ATFP8

2 of 13

Yes

Atha\RA-5

2 of 13

Yes

Atha\RAB1A

2 of 13

Yes

Atha\RAB1C

2 of 13

Yes

1 of 13

1 of 13

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1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\SGP2

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (14)

Scer\GSP1

1 of 13

Yes

Scer\GSP2

1 of 13

Yes

Scer\GTR1

1 of 13

Yes

Scer\GTR2

1 of 13

Yes

Scer\RSR1

1 of 13

Yes

Scer\SEC4

1 of 13

Yes

Scer\TEM1

1 of 13

Yes

Scer\VPS21

1 of 13

Yes

Scer\YPT1

1 of 13

Yes

Scer\YPT6

1 of 13

Yes

Scer\YPT7

1 of 13

Yes

Scer\YPT31

1 of 13

Yes

Scer\YPT32

1 of 13

Yes

Scer\YPT53

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (11)

Spom\gtr1

1 of 12

Yes

Spom\gtr2

1 of 12

Yes

Spom\ryh1

1 of 12

Yes

Spom\spg1

1 of 12

Yes

Spom\spi1

1 of 12

Yes

Spom\ypt1

1 of 12

Yes

Spom\ypt2

1 of 12

Yes

Spom\ypt3

1 of 12

Yes

Spom\ypt4

1 of 12

Yes

Spom\ypt7

1 of 12

Yes

Spom\ypt71

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Rab35. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (50)

4 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Mtl

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Ran

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Ric

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Rab35^S22N.UAS.YFP

ameliorates epilepsy

modeled by para^bss1

(Kroll et al., 2015)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RAB35; RAB35, member RAS oncogene family

11 of 14

604199

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

RNA-protein

Physical Interaction

Assay

References

Rab35 - shep

coimmunoprecipitation, quantitative reverse transcription pcr

(Olesnicky et al., 2018)

protein-protein

Physical Interaction

Assay

References

Rab35 - Gdi

experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting

(Rhee et al., 2014, Guruharsha et al., 2011)

Rab35 - Rab3

experimental knowledge based

(Guruharsha et al., 2011)

Rab35 - Rbpn-5

pull down, peptide massfingerprinting, two hybrid

(Hu et al., 2017.6.13, Gillingham et al., 2014)

Rab35 - Sbf

pull down, western blot, peptide massfingerprinting

(Miao et al., 2019, Gillingham et al., 2014)

Rab35 - Tcs5

anti tag coimmunoprecipitation, anti tag western blot

(Molina et al., 2022)

Rab35 - run

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

Rab35 - sky

gtpase assay

(Uytterhoeven et al., 2011)

Rab35 - sn

anti tag coimmunoprecipitation, anti tag western blot, pull down, molecular weight estimation by staining, anti bait coimmunoprecipitation, western blot

(Zhang et al., 2009)

Rab35 - strat

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Rab35

enhanceable

Rab27

(Camelo et al., 2022)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Ma et al., 2012)

suppressible

(Kroll et al., 2015)

sky

suppressible

Rab35

(Uytterhoeven et al., 2011)

Tcs5

suppressible

Rab35

(Molina et al., 2022)

wkd

suppressible

Rab35

(Schottenfeld-Roames and Ghabrial, 2012)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0031090

MIST (protein-protein) - An integrated Molecular Interaction Database

33014

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Endocytosis

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

gene_with_edited_transcript

(Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-64

Cytogenetic map

19B3-19B3

Sequence location

X:20,155,766..20,159,872 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

19B3-19B3

Limits computationally determined from genome sequence between P{EP}amn^EP1639&P{EP}amn^EP367 and P{EP}CG1702^EP1525

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-64

(FlyBase, 2016)

1-63.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (16)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

32635

w^* P{EP}Rab35^G11110

62559

w¹¹¹⁸ TI{TI}Rab35^EYFP

9821

y¹ w^*; P{UASp-YFP.Rab35}15

28342

y¹ v¹; P{TRiP.JF02978}attP2

9818

y¹ w^*; P{UASp-YFP.Rab35.Q67L}11

9819

y¹ w^*; P{UAST-YFP.Rab35.S22N}01

9820

y¹ w^*; P{UAST-YFP.Rab35.S22N}06

Kyoto

104417

y^* w^* P{GawB}Rab35^NP3216 / FM7c

9575R-3

v330170

v101361

v318284

PBac{fTRG00931.sfGFP-TVPTBF}VK00033

More stocks available...

Genomic Clones (9)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (92)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0031090

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0031090

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Shim et al., 2010)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

A large increase in the proportion of binucleated cells is seen following transfection of S2 cells with dsRNA made from templates generated with primers directed against this gene.

(Kouranti et al., 2006)

S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

(Stroschein-Stevenson et al., 2006)

dsRNA made from templates generated with primers directed against Rab35 that is transfected into S2 treated with Listeria monocytogenes reveals Rab35 to be involved in Listeria monocytogenes entry, intracellular growth, and vacuolar escape.

(Cheng et al., 2005)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in S2R⁺ cells: binucleate cells.

(Kiger et al., 2003)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

CG9575

(Meadows, 2017.1.11, Dunst et al., 2015, Keleman et al., 2009.8.5, Perkins et al., 2009, Sinka et al., 2008, Derré et al., 2007, Zhang et al., 2007, Cermelli et al., 2006, Colicelli, 2004)

DRab35

(Zhang et al., 2005)

DmRab35

(Pereira-Leal and Seabra, 2001, Pereira-Leal and Seabra, 2001)

Q95RH7

(Rojas et al., 2012)

Rab35

(Alvarez and Papagiannouli, 2025, Hodgson et al., 2024, Kim et al., 2024, Lee et al., 2023, Pradhan et al., 2023, Tsarouhas et al., 2023, Camelo et al., 2022, Chen and He, 2022, Kunduri et al., 2022, Linnemannstöns et al., 2022, Molina et al., 2022, Ochi et al., 2022, Rounds et al., 2022, Sanchez-Lopez et al., 2022, Camelo and Luschnig, 2021, Evans et al., 2021, Kohrs et al., 2021, Ma and Brill, 2021, Ricolo et al., 2021, Loerke and Blankenship, 2020, Martin, 2020, Paré and Zallen, 2020, Brantley and Fuller, 2019, Hodgson et al., 2019, Jiang et al., 2019, Johnson and Andrew, 2019, Krämer et al., 2019, Miao et al., 2019, Myat et al., 2019, Bellec et al., 2018, Olesnicky et al., 2018, Beer and Wehman, 2017, Coutinho-Budd et al., 2017, Del Signore et al., 2017, Hu et al., 2017.6.13, Jewett et al., 2017, Caviglia et al., 2016, Meehan et al., 2016, Otani et al., 2016, Sarov et al., 2016, Kroll et al., 2015, White et al., 2015, Corrigan et al., 2014, Fernandes et al., 2014, Gillingham et al., 2014, Gillingham et al., 2014, Gillingham et al., 2014, Beckett et al., 2013, O'Sullivan et al., 2013, Zhang et al., 2013, Hazelett et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Koles et al., 2012, Laflamme et al., 2012, Ma et al., 2012, Schottenfeld-Roames and Ghabrial, 2012, Wang et al., 2012, Yang et al., 2012, Otani et al., 2011, Uytterhoeven et al., 2011, Shim et al., 2010, Tan et al., 2009, Zhang et al., 2009, Sinka et al., 2008, Bakal et al., 2007, Bakal et al., 2007, Derré et al., 2007, Zhang et al., 2007, Zhang et al., 2007, Cermelli et al., 2006, Kouranti et al., 2006, Stroschein-Stevenson et al., 2006)

Rab35 CES

(Kaye et al., 2017)

dRab35

(Carim et al., 2019, Zhang and Scott, 2006)

rab35

(Lammers et al., 2017, Jin et al., 2012, Chan et al., 2011)

Name Synonyms

Rab GTPase

(Paré and Zallen, 2020)

Rab35

Secondary FlyBase IDs

Datasets (1)

Study focus (1)

Experimental Role

Project

Project Type

Title

bait_protein

Rab_Effectors_interaction_map

interactome

Interaction map generated by purification of most Rab factors, with identification of copurifying effectors by mass spectrometry.

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 55 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

33014

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9W5X0

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9W5X0

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0031090

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

33014/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0031090

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0031090

FlyMine - An integrated database for Drosophila genomics

FBgn0031090

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG9575

MARRVEL_MODEL - MARRVEL (model organism gene)

33014

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0031090

DroID - A comprehensive database of gene and protein interactions.

FBgn0031090

DRSC - Results frm RNAi screens

FBgn0031090

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Rab35_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0031090

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0031090

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9575

Flygut - An atlas of the Drosophila adult midgut

FBgn0031090

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0031090

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC015416

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Endocytosis

MIST (protein-protein) - An integrated Molecular Interaction Database

33014

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (125)

The following external sites may use different assemblies or annotations than FlyBase.

-0.66

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