FB2026_02 , released June 18, 2026
Human Disease Model Report: cancer, multiple, modulation by steroid hormone via mir-let7
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General Information
Name
cancer, multiple, modulation by steroid hormone via mir-let7
FlyBase ID
FBhh0000935
Disease Ontology Term
Parent Disease
OMIM
Overview

In two different Drosophila models of cancer assayed at the larval stage (see FBhh0000779 and FBhh0000772), it has been observed that tumorigenic cells are transformed into nontumorigenic cells after metamorphosis, and are eventually evicted from adult tissues. It has been shown that this process is dependent upon the increased ecdysone signalling that normally occurs during metamorphosis and into adulthood, and is mediated by the ecdysone-responsive microRNA mir-let7 and its target transcription factor gene chinmo. In these systems, reduction in the levels of either mir-let7 or chinmo results in persistence of tumorigenic cells and metastatic tumor growth in adults.

The human let7 microRNA family has been described as serving tumor suppressor functions, with downregulation observed in various cancers. There are at least 10 members of this family in human; several are encoded by multiple genes. There are also multiple microRNA genes in Drosophila categorized as within this family (as defined by shared 5' seed sequences).

Ecdysone (20-hydroxyecdysone) is the major steroid hormone in insects and plays essential roles in coordinating developmental transitions such as larval molting and metamorphosis; it is a member of a more general class called ecdysteroids. In Drosophila, ecdysone-regulated genes are bound by a heterodimer of ecdysone receptor (encoded by EcR) and Ultraspiracle (encoded by usp). In animals carrying a dominant-negative form of EcR, larval-derived tumorigenic cells continue to grow in adults and result in the accumulation of large tumors throughout the body; similar results are obtained in animals with RNAi-effected knockdown of usp.

[updated Dec. 2018 by FlyBase; FBrf0222196]

Disease Summary Information
Disease Summary: cancer, multiple, modulation by steroid hormone via mir-let7
OMIM report
Human gene(s) implicated
Symptoms and phenotype
Genetics

In human, 10 members of the let-7 family have been identified (Thammaiah and Jayaram, 2016 pubmed:30159414).

(FlyBase Curators, 2013-)
Cellular phenotype and pathology

The expression of let-7 family is required for developmental timing and tumor suppressor function; it must be suppressed for the self-renewal of stem cells (Lee et al., 2016; pubmed:26399619).

(FlyBase Curators, 2013-)

In various types of human cancer, downregulation of let-7 has been observed (Thammaiah and Jayaram, 2016 pubmed:30159414).

(FlyBase Curators, 2013-)
Molecular information

Higher animals have multiple isoforms of let-7 miRNAs encoded by multiple genes within the let-7 miRNA family; these isoforms share a consensus sequence called the 'seed sequence' (Lee et al., 2016; pubmed:26399619).

(FlyBase Curators, 2013-)
External links
Disease synonyms
cancer, multiple, let-7-related
cancer, multiple, modulation by steroid hormone via let-7
Search term: cancer, microRNA-related
Ortholog Information
Human gene(s) in FlyBase
    Other mammalian ortholog(s) used
      D. melanogaster Gene Information (4)
      Dmel\EcR
      Gene Snapshot
      Ecdysone receptor (EcR) encodes a protein that interacts with the product of usp to form the nuclear ecdysone receptor heterodimer, which modulates, in conjunction with co-activators and co-repressors, the activities of hundreds of genes in a tissue- and stage-specific way. EcR is widely expressed in embryonic and larval tissues and in some adult tissues where its activities (modulated by the hormone ecdysone) trigger both molting and metamorphosis. [Date last reviewed: 2019-03-07]
      Molecular function (GO)
      Cellular component (GO)
      Gene Groups / Pathways
      Comments on ortholog(s)

      Low- to moderate-scoring ortholog of human NR1H2, NR1H3, NR1H4 (1 Drosophila to 3 human). Dmel\EcR shares 33-38% identity and 47-55% similarity with the human genes.

      Orthologs and Alignments from DRSC
      DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans
      H. sapiens (Human)
      Dmel\usp
      Gene Snapshot
      Contributions welcome
      Molecular function (GO)
      Cellular component (GO)
      Gene Groups / Pathways
      Comments on ortholog(s)
      Orthologs and Alignments from DRSC
      DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans
      H. sapiens (Human)
      Dmel\chinmo
      Gene Snapshot
      Chronologically inappropriate morphogenesis (chinmo) encodes a putative BTB-zinc finger transcription factor. It is essential for neuronal temporal patterning. It also plays a role in the regulation of eye development, tumor formation, and the self-renewal and/or sexual identity of male somatic cyst stem cells by acting downstream of JAK-STAT signaling. [Date last reviewed: 2019-03-07]
      Molecular function (GO)
        Cellular component (GO)
        Gene Groups / Pathways
        Comments on ortholog(s)

        Encodes several isoforms of a BTB-zinc finger transcription factor with low levels of homology to several human ZBTB genes.

        Orthologs and Alignments from DRSC
        DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans
        H. sapiens (Human)
        Dmel\mir-let7
        Gene Snapshot
        Contributions welcome
        Molecular function (GO)
        Cellular component (GO)
        Gene Groups / Pathways
        Comments on ortholog(s)

        5' seed sequence homologous to that of human let-7 microRNAs; several other Drosophila miRNAs have 5' seed sequence homologous to human let-7's, including mir-963, mir-977 and mir-984 (FBrf0242402).

        Orthologs and Alignments from DRSC
        DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans
        H. sapiens (Human)
        Other Genes Used: Viral, Bacterial, Synthetic (0)
          Summary of Physical Interactions (146 groups)
          EcR
          protein-protein
          Interacting group
          Assay
          References
          EcR
          bimolecular fluorescence complementation, fluorescence microscopy
          (Dutko-Gwóźdź et al., 2008)
          EcR - 14-3-3ε
          fluorescent resonance energy transfer, fluorescence microscopy
          (Ng et al., 2017)
          EcR - alien
          pull down, autoradiography, anti tag coimmunoprecipitation, anti tag western blot
          (Huang et al., 2014, Dressel et al., 1999)
          EcR - ari-1
          two hybrid, enzymatic study, western blot, anti tag coimmunoprecipitation, anti tag western blot
          (Gradilla et al., 2011)
          EcR - Art1
          anti tag coimmunoprecipitation, western blot
          (Kimura et al., 2008)
          EcR - Art4
          anti tag coimmunoprecipitation, western blot
          (Cakouros et al., 2004)
          EcR - bon
          pull down, anti tag western blot
          (Beckstead et al., 2001)
          EcR - brm
          anti bait coimmunoprecipitation, western blot, proximity-dependent biotin identification, Identification by mass spectrometry
          (Mazina et al., 2020)
          EcR - Cdk8
          anti bait coimmunoprecipitation, western blot, two hybrid, Identification by mass spectrometry
          (Xie et al., 2015)
          EcR - cg
          proximity ligation assay
          (Hitrik et al., 2016)
          EcR - CG10209
          two hybrid array
          (Shokri et al., 2019)
          EcR - Chd64
          two hybrid, pull down, autoradiography
          (Li et al., 2007)
          EcR - Chro
          anti bait coimmunoprecipitation, western blot, proximity-dependent biotin identification, Identification by mass spectrometry
          (Mazina et al., 2020)
          EcR - Cp190
          anti bait coimmunoprecipitation, western blot, proximity-dependent biotin identification, Identification by mass spectrometry
          (Mazina et al., 2020)
          EcR - crc
          two hybrid, pull down, autoradiography
          (Gauthier et al., 2012)
          EcR - CSN4
          anti tag coimmunoprecipitation, anti tag western blot
          (Huang et al., 2014)
          EcR - CSN5
          anti tag coimmunoprecipitation, anti tag western blot
          (Huang et al., 2014)
          EcR - CTCF
          anti bait coimmunoprecipitation, western blot
          (Pascual-Garcia et al., 2017)
          EcR - CycC
          anti bait coimmunoprecipitation, Identification by mass spectrometry
          (Xie et al., 2015)
          EcR - Dek
          anti tag coimmunoprecipitation, western blot, pull down
          (Sawatsubashi et al., 2010)
          EcR - E(bx)
          pull down, autoradiography
          (Badenhorst et al., 2005)
          EcR - ERR
          anti tag coimmunoprecipitation, anti tag western blot
          (Kovalenko et al., 2019)
          EcR - Fkbp39
          two hybrid, pull down, autoradiography
          (Li et al., 2007)
          EcR - fru
          anti tag coimmunoprecipitation, anti tag western blot
          (Zhang et al., 2018)
          EcR - Hr38
          bimolecular fluorescence complementation
          (Zoglowek et al., 2012)
          EcR - Hsc70-4
          anti bait coimmunoprecipitation, Identification by mass spectrometry
          (Xie et al., 2015)
          EcR - Jra
          pull down, western blot, proximity ligation assay, fluorescence microscopy
          (Yoo et al., 2021)
          EcR - kay
          pull down, western blot
          (Yoo et al., 2021)
          EcR - Kdm4A
          anti tag coimmunoprecipitation, western blot
          (Tsurumi et al., 2013)
          EcR - kto
          anti bait coimmunoprecipitation, Identification by mass spectrometry
          (Xie et al., 2015)
          EcR - LKRSDH
          pull down, autoradiography, peptide massfingerprinting, anti bait coimmunoprecipitation, western blot
          (Cakouros et al., 2008)
          EcR - Lpt
          pull down, western blot, anti tag coimmunoprecipitation
          (Chauhan et al., 2012)
          EcR - MBD-like
          two hybrid array
          (Shokri et al., 2019)
          EcR - MED1
          anti bait coimmunoprecipitation, Identification by mass spectrometry
          (Xie et al., 2015)
          EcR - MED14
          anti bait coimmunoprecipitation, Identification by mass spectrometry, two hybrid
          (Xie et al., 2015)
          EcR - MED15
          anti bait coimmunoprecipitation, Identification by mass spectrometry
          (Xie et al., 2015)
          EcR - MED26
          anti bait coimmunoprecipitation, Identification by mass spectrometry
          (Xie et al., 2015)
          EcR - MED27
          anti tag coimmunoprecipitation, western blot
          (Homem et al., 2014)
          EcR - Met
          pull down, autoradiography, two hybrid, anti tag coimmunoprecipitation, anti tag western blot
          (Long et al., 2025, Bitra and Palli, 2009, Li et al., 2007)
          EcR - Mgtor
          anti bait coimmunoprecipitation, western blot
          (Pascual-Garcia et al., 2017)
          EcR - Mi-2
          anti bait coimmunoprecipitation, western blot, molecular weight estimation by staining, proximity-dependent biotin identification, Identification by mass spectrometry, pull down, autoradiography
          (Mazina et al., 2020, Brehm, 2017.5.22, Kreher et al., 2017)
          EcR - mor
          proximity-dependent biotin identification, Identification by mass spectrometry, anti bait coimmunoprecipitation, western blot
          (Mazina et al., 2020)
          EcR - nej
          anti tag coimmunoprecipitation, anti tag western blot
          (Kirilly et al., 2011)
          EcR - Nelf-A
          anti bait coimmunoprecipitation, western blot, proximity-dependent biotin identification, Identification by mass spectrometry
          (Mazina et al., 2021, Mazina et al., 2020)
          EcR - NELF-B
          anti bait coimmunoprecipitation, western blot
          (Mazina et al., 2021)
          EcR - Nelf-E
          anti bait coimmunoprecipitation, western blot
          (Mazina et al., 2021)
          EcR - Nup98-96
          anti bait coimmunoprecipitation, western blot
          (Pascual-Garcia et al., 2017)
          EcR - Nup358
          proximity-dependent biotin identification, Identification by mass spectrometry, anti bait coimmunoprecipitation, western blot
          (Mazina et al., 2020)
          EcR - osa
          proximity-dependent biotin identification, Identification by mass spectrometry, anti bait coimmunoprecipitation, western blot
          (Mazina et al., 2020)
          EcR - Parp1
          anti tag coimmunoprecipitation, western blot
          (Sawatsubashi et al., 2004)
          EcR - Rel
          two hybrid array
          (Shokri et al., 2019)
          EcR - rig
          anti bait coimmunoprecipitation, Identification by mass spectrometry, pull down, autoradiography
          (Xie et al., 2015, Gates et al., 2004)
          EcR - skd
          anti bait coimmunoprecipitation, Identification by mass spectrometry
          (Xie et al., 2015)
          EcR - Smr
          anti bait coimmunoprecipitation, Identification by mass spectrometry, two hybrid
          (Xie et al., 2015, Wang et al., 2006)
          EcR - Spt5
          anti bait coimmunoprecipitation, western blot, proximity-dependent biotin identification, Identification by mass spectrometry
          (Mazina et al., 2020)
          EcR - svp
          two hybrid
          (Zelhof et al., 1995)
          EcR - tai
          anti bait coimmunoprecipitation, Identification by mass spectrometry, pull down, autoradiography
          (Xie et al., 2015, Bai et al., 2000)
          EcR - TP53INP
          anti tag coimmunoprecipitation, western blot, pull down, anti bait coimmunoprecipitation, Identification by mass spectrometry
          (Xie et al., 2015, Francis et al., 2010)
          EcR - Trf
          two hybrid array
          (Shokri et al., 2019)
          EcR - trr
          anti bait coimmunoprecipitation, western blot
          (Pascual-Garcia et al., 2017)
          EcR - twi
          anti tag coimmunoprecipitation, anti tag western blot
          (Ruan et al., 2024)
          EcR - usp
          two hybrid, pull down, western blot, anti bait coimmunoprecipitation, Identification by mass spectrometry, anti tag coimmunoprecipitation, anti tag western blot, electrophoretic mobility shift assay, autoradiography, peptide massfingerprinting, experimental knowledge based, bimolecular fluorescence complementation, fluorescence microscopy, electrophoretic mobility supershift assay, x-ray crystallography
          (Ruan et al., 2024, Yoo et al., 2021, Kreher et al., 2017, Xie et al., 2015, Huang et al., 2014, Rhee et al., 2014, Bitra and Palli, 2009, Dutko-Gwóźdź et al., 2008, Li et al., 2007, Fang et al., 2005, Bergman et al., 2004, Maki et al., 2004, Przibilla et al., 2004, Devarakonda et al., 2003, Hu et al., 2003, Lezzi et al., 2002, Sutherland et al., 1995, Zelhof et al., 1995, Yao et al., 1993, Yao et al., 1992)
          EcR - Usp7
          pull down, autoradiography
          (van der Knaap et al., 2010)
          EcR - Utx
          anti tag coimmunoprecipitation, western blot, pull down, autoradiography
          (Denton et al., 2013)
          EcR - yki
          coimmunoprecipitation, western blot
          (Zhang et al., 2015)
          RNA-protein
          Interacting group
          Assay
          References
          EcR - B52
          electrophoretic mobility shift assay, autoradiography, systematic evolution of ligands by exponential enrichment, full identification by DNA sequencing
          (Kim et al., 2003)
          RNA-RNA
          Interacting group
          Assay
          References
          EcR - mir-14
          fluorescence technology, luminiscence technology, necessary binding region, western blot, immunohistochemistry
          (Varghese and Cohen, 2007)
          EcR - mir-34
          immunohistochemistry
          (Lai et al., 2016)
          usp
          protein-protein
          Interacting group
          Assay
          References
          usp
          bimolecular fluorescence complementation, fluorescence microscopy, anti tag coimmunoprecipitation, anti tag western blot, two hybrid
          (Bitra and Palli, 2009, Dutko-Gwóźdź et al., 2008, Fang et al., 2005)
          usp - ari-1
          two hybrid
          (Gradilla et al., 2011)
          usp - bon
          pull down, anti tag western blot
          (Beckstead et al., 2001)
          usp - Cdk8
          anti bait coimmunoprecipitation, Identification by mass spectrometry, western blot
          (Xie et al., 2015)
          usp - Chd64
          two hybrid, pull down, autoradiography
          (Li et al., 2007)
          usp - CycC
          anti bait coimmunoprecipitation, Identification by mass spectrometry
          (Xie et al., 2015)
          usp - EcR
          two hybrid, pull down, western blot, anti bait coimmunoprecipitation, Identification by mass spectrometry, anti tag coimmunoprecipitation, anti tag western blot, electrophoretic mobility shift assay, autoradiography, peptide massfingerprinting, experimental knowledge based, bimolecular fluorescence complementation, fluorescence microscopy, electrophoretic mobility supershift assay, x-ray crystallography
          (Ruan et al., 2024, Yoo et al., 2021, Kreher et al., 2017, Xie et al., 2015, Huang et al., 2014, Rhee et al., 2014, Bitra and Palli, 2009, Dutko-Gwóźdź et al., 2008, Li et al., 2007, Fang et al., 2005, Bergman et al., 2004, Maki et al., 2004, Przibilla et al., 2004, Devarakonda et al., 2003, Hu et al., 2003, Lezzi et al., 2002, Sutherland et al., 1995, Zelhof et al., 1995, Yao et al., 1993, Yao et al., 1992)
          usp - egg
          two hybrid
          (Stabell et al., 2006)
          usp - Fkbp39
          pull down, autoradiography, two hybrid
          (Li et al., 2007)
          usp - foxo
          pull down, anti tag western blot, anti bait coimmunoprecipitation, western blot
          (Koyama et al., 2014)
          usp - fru
          anti tag coimmunoprecipitation, anti tag western blot
          (Zhang et al., 2018)
          usp - Hr38
          pull down, western blot, bimolecular fluorescence complementation, two hybrid
          (Zoglowek et al., 2012, Sutherland et al., 1995)
          usp - Hsc70-4
          anti bait coimmunoprecipitation, Identification by mass spectrometry
          (Xie et al., 2015)
          usp - Jra
          pull down, western blot
          (Yoo et al., 2021)
          usp - kto
          anti bait coimmunoprecipitation, Identification by mass spectrometry
          (Xie et al., 2015)
          usp - Lpt
          pull down, western blot, anti tag coimmunoprecipitation
          (Chauhan et al., 2012)
          usp - MED1
          anti bait coimmunoprecipitation, Identification by mass spectrometry
          (Xie et al., 2015)
          usp - MED14
          anti bait coimmunoprecipitation, Identification by mass spectrometry
          (Xie et al., 2015)
          usp - MED15
          anti bait coimmunoprecipitation, Identification by mass spectrometry
          (Xie et al., 2015)
          usp - MED16
          anti bait coimmunoprecipitation, Identification by mass spectrometry
          (Xie et al., 2015)
          usp - MED17
          anti bait coimmunoprecipitation, Identification by mass spectrometry
          (Xie et al., 2015)
          usp - MED23
          anti bait coimmunoprecipitation, Identification by mass spectrometry
          (Xie et al., 2015)
          usp - MED25
          anti bait coimmunoprecipitation, Identification by mass spectrometry
          (Xie et al., 2015)
          usp - MED30
          anti bait coimmunoprecipitation, Identification by mass spectrometry
          (Xie et al., 2015)
          usp - Met
          two hybrid, pull down, autoradiography
          (Bitra and Palli, 2009, Li et al., 2007)
          usp - rig
          anti bait coimmunoprecipitation, Identification by mass spectrometry, pull down, autoradiography
          (Xie et al., 2015, Gates et al., 2004)
          usp - skd
          anti bait coimmunoprecipitation, Identification by mass spectrometry
          (Xie et al., 2015)
          usp - Smr
          anti bait coimmunoprecipitation, Identification by mass spectrometry
          (Xie et al., 2015)
          usp - Sumo
          two hybrid, anti tag coimmunoprecipitation, comigration in sds page, anti tag western blot
          (Pirone et al., 2017, Wang et al., 2014)
          usp - tai
          pull down, autoradiography, anti bait coimmunoprecipitation, Identification by mass spectrometry
          (Xie et al., 2015, Bai et al., 2000)
          usp - Utx
          anti bait coimmunoprecipitation, Identification by mass spectrometry, pull down, autoradiography
          (Xie et al., 2015, Denton et al., 2013)
          chinmo
          RNA-protein
          Interacting group
          Assay
          References
          chinmo - brat
          anti tag coimmunoprecipitation, primer specific pcr
          (Loedige et al., 2015)
          chinmo - Caper
          anti tag coimmunoprecipitation, full identification by RNA sequencing, anti bait coimmunoprecipitation, quantitative reverse transcription pcr
          (Titus et al., 2023)
          chinmo - Imp
          pull down, western blot
          (Genovese et al., 2019)
          chinmo - Syp
          pull down, western blot
          (Genovese et al., 2019)
          protein-protein
          Interacting group
          Assay
          References
          chinmo
          two hybrid, molecular sieving
          (Bonchuk et al., 2024, Rinehart et al., 2024)
          chinmo - ab
          two hybrid
          (Bonchuk et al., 2024)
          chinmo - Ada2b
          anti tag coimmunoprecipitation, peptide massfingerprinting
          (Weake et al., 2011)
          chinmo - bab1
          two hybrid
          (Bonchuk et al., 2024)
          chinmo - bab2
          two hybrid
          (Bonchuk et al., 2024)
          chinmo - br
          two hybrid, two hybrid array
          (Bonchuk et al., 2024, Shokri et al., 2019)
          chinmo - CG3726
          two hybrid
          (Bonchuk et al., 2024)
          chinmo - CG4318
          two hybrid array
          (Shokri et al., 2019)
          chinmo - CG6118
          two hybrid
          (Bonchuk et al., 2024)
          chinmo - CG6765
          two hybrid
          (Bonchuk et al., 2024)
          chinmo - CG8119
          two hybrid array
          (Shokri et al., 2019)
          chinmo - CG8924
          two hybrid
          (Bonchuk et al., 2024)
          chinmo - CG10147
          two hybrid array
          (Shokri et al., 2019)
          chinmo - CG10669
          two hybrid array
          (Shokri et al., 2019)
          chinmo - CG11180
          two hybrid
          (Rinehart et al., 2024)
          chinmo - CG11723
          two hybrid array
          (Shokri et al., 2019)
          chinmo - CG12236
          two hybrid
          (Bonchuk et al., 2024)
          chinmo - CG15812
          two hybrid
          (Bonchuk et al., 2024)
          chinmo - fru
          two hybrid
          (Bonchuk et al., 2024)
          chinmo - Hmg-2
          two hybrid array
          (Shokri et al., 2019)
          chinmo - hth
          two hybrid array
          (Shokri et al., 2019)
          chinmo - lolal
          two hybrid
          (Bonchuk et al., 2024)
          chinmo - lov
          two hybrid
          (Bonchuk et al., 2024)
          chinmo - mamo
          two hybrid
          (Bonchuk et al., 2024)
          chinmo - MBD-R2
          two hybrid array
          (Shokri et al., 2019)
          chinmo - p53
          two hybrid array
          (Shokri et al., 2019)
          chinmo - psq
          two hybrid
          (Bonchuk et al., 2024)
          chinmo - Rbf2
          two hybrid array
          (Shokri et al., 2019)
          chinmo - rib
          two hybrid
          (Bonchuk et al., 2024)
          chinmo - scro
          two hybrid array
          (Shokri et al., 2019)
          chinmo - Sirt1
          anti tag coimmunoprecipitation, anti tag western blot
          (Pandey et al., 2021)
          chinmo - Sox21a
          array technology, two hybrid array
          (Shokri et al., 2019)
          chinmo - ttk
          two hybrid
          (Bonchuk et al., 2024)
          mir-let7
          RNA-protein
          Interacting group
          Assay
          References
          mir-let7 - AGO1
          nucleic acid uv cross-linking assay, molecular weight estimation by staining, autoradiography, anti bait coimmunoprecipitation, northern blot, pull down, western blot
          (Kakumani et al., 2020, Lim et al., 2020, Lim et al., 2018, Ameres et al., 2011, Tomari et al., 2007)
          mir-let7 - Dcr-1
          enzymatic study, autoradiography, electrophoretic mobility shift assay, competition binding
          (Pandey et al., 2022, Tsutsumi et al., 2011, Liu et al., 2007, Ye et al., 2007, Jiang et al., 2005)
          mir-let7 - drosha
          enzymatic study, autoradiography
          (Pandey et al., 2022)
          mir-let7 - Fmr1
          anti bait coimmunoprecipitation, quantitative reverse transcription pcr
          (Yang et al., 2009)
          mir-let7 - Hen1
          enzymatic study, autoradiography
          (Mickute et al., 2018)
          mir-let7 - Unr
          pull down, western blot
          (Kakumani et al., 2020)
          RNA-RNA
          Interacting group
          Assay
          References
          mir-let7 - ab
          immunohistochemistry, luminiscence technology
          (Kucherenko et al., 2012, Burgler and Macdonald, 2005)
          mir-let7 - cwo
          luminiscence technology, necessary binding region, quantitative reverse transcription pcr
          (Chen et al., 2014)
          mir-let7 - Dp
          western blot, luminiscence technology, necessary binding region
          (Gehrke et al., 2010)
          mir-let7 - Imp
          immunohistochemistry, fluorescence technology, necessary binding region
          (Toledano et al., 2012)
          mir-let7 - wech
          luminiscence technology, necessary binding region
          (Wakiyama et al., 2007)
          Alleles Reported to Model Human Disease (Disease Ontology) (14 alleles)
          EcR
          Models Based on Experimental Evidence ( 0 )
          Allele
          Disease
          Evidence
          References
          Modifiers Based on Experimental Evidence ( 7 )
          usp
          Models Based on Experimental Evidence ( 0 )
          Allele
          Disease
          Evidence
          References
          Modifiers Based on Experimental Evidence ( 1 )
          Allele
          Disease
          Interaction
          References
          uspJF02546
          exacerbates  cancer
          modeled by ph-d505, ph-p505
          (Jiang et al., 2018)
          chinmo
          Models Based on Experimental Evidence ( 3 )
          Modifiers Based on Experimental Evidence ( 3 )
          Allele
          Disease
          Interaction
          References
          chinmoHMS00036
          ameliorates  cancer
          modeled by ph-d505, ph-p505
          (Jiang et al., 2018)
          chinmo1
          ameliorates  cancer
          modeled by Ras85DV12.UAS
          (Enomoto et al., 2021)
          chinmoUAS.fl
          exacerbates  cancer
          modeled by ph-d505, ph-p505
          (Jiang et al., 2018)
          mir-let7
          Models Based on Experimental Evidence ( 0 )
          Allele
          Disease
          Evidence
          References
          Modifiers Based on Experimental Evidence ( 3 )
          Alleles Representing Disease-Implicated Variants
          Genetic Tools, Stocks and Reagents
          Sources of Stocks
          Contact lab of origin for a reagent not available from a public stock center.
          Bloomington Stock Center Disease Page
          Related mammalian, viral, bacterial, or synthetic transgenes
          Allele
          Transgene
          Publicly Available Stocks
          Selected Drosophila transgenes
          Allele
          Transgene
          Publicly Available Stocks
          EcRDN.UAS.cUa
          P{UAS-EcR.DN.U}
          EcRB1.UAS
          P{UAS-EcR.B1}
          w*; P{UAS-EcR.B1}3b
          EcRB2-ΔC655.F645A.UAS
          P{UAS-EcR.B2.F645A}
          w*; P{UAS-EcR.B2.F645A}TP1
          chinmoUAS.fl
          P{UAS-chinmo.fl}
          y1 w*; βTub60DPin-1/CyO, y+; P{UAS-chinmo.fl}3
          mir-let7UAS.cCa
          P{UAS-mir-let7.C}
          mir-let7UAS.cGa
          P{UAS-mir-let7.pre}
          mir-let7sponge.V2.UAS.mCherry
          P{UAS-mCherry.mir-let7.sponge.V2}
          w*; P{UAS-mCherry.mir-let7.sponge.V2}attP40; P{UAS-mCherry.mir-let7.sponge.V2}attP2/TM6B, Tb1
          RNAi constructs available
          Allele
          Transgene
          Publicly Available Stocks
          uspRNAi.UAS.cUa
          P{UAS-usp.RNAi.U}
          uspVSH330777
          P{VSH330777}
          uspGD1554
          P{GD1554}
          w1118; P{GD1554}v16893/TM3
          uspJF02546
          P{TRiP.JF02546}
          y1 v1; P{TRiP.JF02546}attP2
          uspHMS01620
          P{TRiP.HMS01620}
          y1 sc* v1 sev21; P{TRiP.HMS01620}attP2
          uspRNAi.UAS.cCa
          P{UAS-usp.RNAi.C}
          uspNIG.4380R
          P{NIG.4380R}
          P{NIG.4380R}1
          usp574.RNAi.UAS
          P{UAS-Usp-RNAi.574}
          uspRNAi.UAS.cLa
          P{UAS-usp.RNAi.L}
          EcRRNAi.UAS.cUa
          P{UAS-EcR.RNAi.U}
          EcRTH01841.N
          P{TH01841.N}
          EcRRNAi.hs.PL
          P{hs-EcR.RNAi.L}
          w1118; P{hs-EcR.RNAi.L}11
          EcRRNAi.B1.UAS.cSa
          P{UAS-EcR.RNAi.S}
          EcRRNAi.A.UAS
          P{UAS-EcR.A.RNAi}
          w1118; P{UAS-EcR.A.RNAi}91/TM3, P{ActGFP}JMR2, Ser1
          EcRRNAi.B1.UAS
          P{UAS-EcR.B1.RNAi}
          w1118; P{UAS-EcR.B1.RNAi}168
          EcRGD1428
          P{GD1428}
          w1118; P{GD1428}v37058
          EcRJF02538
          P{TRiP.JF02538}
          y1 v1; P{TRiP.JF02538}attP2
          EcRRNAi.UAS.cIa
          P{UAS-EcR.RNAi.I}
          EcRRNAi.UAS.cCa
          P{UAS-EcR.RNAi.C}
          w1118; P{UAS-EcR.RNAi.C}97
          EcRNIG.1765R
          P{NIG.1765R}
          P{NIG.1765R}2
          EcRRNAi.UAS
          P{UAS-EcR.RNAi}
          EcRHMC03114
          P{TRiP.HMC03114}
          y1 v1; P{TRiP.HMC03114}attP2/TM3, Sb1
          EcRHMJ22371
          P{TRiP.HMJ22371}
          y1 v1; P{TRiP.HMJ22371}attP40/CyO
          chinmoVSH330501
          P{VSH330501}
          P{VSH330501}attP40
          chinmoRNAi.UAS.cLa
          P{UAS-chinmo.RNAi.L}
          chinmoRNAi.lexAop
          P{lexAop-chinmo.RNAi}
          chinmoRNAi.UAS.cCa
          PBac{UAS-chinmo.RNAi.C}
          chinmoGD10323
          P{GD10323}
          chinmoJF02341
          P{TRiP.JF02341}
          y1 v1; P{TRiP.JF02341}attP2
          chinmoHM04048
          P{TRiP.HM04048}
          y1 v1; P{TRiP.HM04048}attP2
          chinmoHMS00036
          P{TRiP.HMS00036}
          y1 v1; P{TRiP.HMS00036}attP2/TM3, Sb1
          chinmoNIG.17156R
          P{NIG.17156R}
          P{NIG.17156R}1
          chinmoHMC05346
          P{TRiP.HMC05346}
          y1 sc* v1 sev21; P{TRiP.HMC05346}attP40
          Selected Drosophila classical alleles
          Allele
          Allele class
          Mutagen
          Publicly Available Stocks
          usp2
          amorphic allele - genetic evidence
          X ray
          usp2/FM7a
          EcRKG04522
          P-element activity
          EcRMI05320-FlpStop.D
          loss of function allele
          phiC31 integrase
          EcRM554fs
          amorphic allele - genetic evidence
          EcRM554fs/SM6b
          EcRV559fs
          loss of function allele
          ethyl methanesulfonate
          y1 w1; EcRV559fs/CyO, y+
          EcRC300Y
          loss of function allele
          EcR112
          amorphic allele - genetic evidence
          Delta2-3 transposase
          y1 w*; P{lacW}EcR112/CyO, y+
          EcR31
          amorphic allele - genetic evidence
          Delta2-3 transposase
          y1 w1; EcR31/CyO, y+
          EcR99
          amorphic allele - genetic evidence
          Delta2-3 transposase
          EcR10-2
          loss of function allele
          EcRQ50st
          amorphic allele - genetic evidence
          ethyl methanesulfonate
          EcRQ50st/SM6b
          chinmoCRISPRΔ1
          loss of function allele
          CRISPR/Cas9
          chinmo1
          w*; chinmo1 P{neoFRT}40A/CyO, y+
          mir-let7KO.C
          amorphic allele - molecular evidence
          ends-out gene targeting
          References (5)