FlyBase Gene Report: Dmel\Ack

General Information

Symbol

Dmel\Ack

Species

D. melanogaster

Name

Activated Cdc42 kinase

Annotation Symbol

CG14992

Feature Type

protein_coding_gene

FlyBase ID

FBgn0028484

Gene Model Status

Current

Stock Availability

14 publicly available

Enzyme Name (EC)

non-specific protein-tyrosine kinase (2.7.10.2)

Gene Summary

Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in diverse biological functions such as cell survival, cell differentiation, cell growth and proliferation (PubMed:11997505, PubMed:18816840, PubMed:22615583, PubMed:23562806, PubMed:25223282, PubMed:27462444). Phosphorylates SH3PX1 and ex (PubMed:11773052, PubMed:22615583, PubMed:27462444). Phosphorylates SH3PX1 predominantly on 'Tyr-56', which likely promotes the recruitment of SH3PX1 to an axonal guidance receptor complex that includes dock and Dscam; because phosphorylation of SH3PX1 increases its interaction with the complex member dock while decreasing its interaction with the actin cytoskeleton modulator WASp (PubMed:11773052). In the wing and eye, promotes tissue growth, and during embryogenesis coordinates cell shape changes required for correct dorsal closure (PubMed:18816840, PubMed:22615583, PubMed:27462444). Functions in the negative regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway by enhancing yki activity thereby promoting cell proliferation and inhibiting apoptosis (PubMed:22615583, PubMed:27462444). This is accomplished, at least in part, by phosphorylating ex thereby reducing its ability to efficiently activate the Hippo signaling cascade (PubMed:27462444). In the eye disk, wing disk and possibly spermatids, inhibits programmed cell death induced by hid and rpr through a mechanism that is independent of the MAP kinase signal transduction pathway (PubMed:22615583). Essential for male and female fertility (PubMed:22615583, PubMed:23562806). During oogenesis required for the correct temporal assembly, and consequently the catalytic activity of long Ctps filaments (cytoophidium) in the germline nurse cells, likely by phosphorylating an unidentified substrate that is essential for linking individual Ctps filaments into large, catalytically active assemblies (PubMed:25223282). (UniProt, Q9VZI2)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Pathway
Gene Group
UniProtKB
Interactive Fly

Also Known As

DACK, p145

Key Links

Genomic Location

Cytogenetic map

64A6-64A6

Sequence location

3L:4,108,186..4,114,157 [+]

Recombination map

3-10

RefSeq locus

NT_037436 REGION:4108186..4114157

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (25 terms)

Molecular Function (8 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

enables DNA-binding transcription factor binding

inferred from physical interaction with UniProtKB:Q45VV3

(Hu et al., 2016)

enables non-membrane spanning protein tyrosine kinase activity

inferred from direct assay

(Worby et al., 2002)

enables protein binding

inferred from physical interaction with UniProtKB:Q08012

(Schoenherr et al., 2012)

inferred from physical interaction with UniProtKB:Q8IPW2

(Abdallah et al., 2013, Worby et al., 2002)

inferred from physical interaction with UniProtKB:Q07436

(Hu et al., 2016)

enables protein tyrosine kinase activity

inferred from direct assay

(Hu et al., 2016, Zahedi et al., 2008)

inferred from mutant phenotype

(Clemens et al., 2000)

enables SH2 domain binding

inferred from direct assay

(Schmucker et al., 2000)

inferred from direct assay

(Abdallah et al., 2013)

enables SH3 domain binding

inferred from direct assay

(Worby et al., 2002)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR017441

(InterPro Project Members, 2004-)

enables protein kinase activity

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR001245, InterPro:IPR008266

(InterPro Project Members, 2004-)

enables protein tyrosine kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN002521457

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR020635

(InterPro Project Members, 2004-)

Biological Process (11 terms)

Terms Based on Experimental Evidence (11 terms)

CV Term

Evidence

References

involved_in cellular response to epidermal growth factor stimulus

inferred from mutant phenotype

(Schoenherr et al., 2012)

involved_in CTP biosynthetic process

inferred from mutant phenotype

(Strochlic et al., 2014)

involved_in dorsal closure, amnioserosa morphology change

inferred from genetic interaction with UniProtKB:Q9I7F7

(Zahedi et al., 2008)

involved_in dorsal closure

inferred from mutant phenotype

(Sem et al., 2002)

involved_in head involution

inferred from genetic interaction with UniProtKB:Q9I7F7

(Zahedi et al., 2008)

involved_in intracellular protein localization

inferred from mutant phenotype

(Abdallah et al., 2013)

involved_in myosin filament assembly

inferred from mutant phenotype

(Zahedi et al., 2008)

involved_in negative regulation of apoptotic process

inferred from genetic interaction with FLYBASE:hid; FB:FBgn0003997

(Schoenherr et al., 2012)

involved_in negative regulation of hippo signaling

inferred from mutant phenotype

(Hu et al., 2016)

involved_in sperm individualization

inferred from mutant phenotype

(Schoenherr et al., 2012)

involved_in spermatid development

inferred from mutant phenotype

(Abdallah et al., 2013)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (6 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

located_in cell periphery

inferred from direct assay

(Abdallah et al., 2013)

located_in clathrin-coated vesicle

inferred from direct assay

(Abdallah et al., 2013)

located_in cytoophidium

inferred from direct assay

(Strochlic et al., 2014)

located_in cytoplasm

inferred from direct assay

(Schoenherr et al., 2012)

part_of protein-containing complex

inferred from direct assay

(Schoenherr et al., 2012)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN002521457

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

NON-RECEPTOR TYROSINE KINASES

Pathway (FlyBase)

NEGATIVE REGULATORS OF HIPPO SIGNALING PATHWAY

Protein Family (UniProt)

Belongs to the protein kinase superfamily. Tyr protein kinase family. (Q9VZI2)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

protein tyrosine kinase activity

RHEA 10596: non-membrane spanning protein tyrosine kinase activity

L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+) (2.7.10.2)

Summaries

Gene Group (FlyBase)

NON-RECEPTOR TYROSINE KINASES -: Non-Receptor Tyrosine kinases (nRTK) are cytoplasmic protein kinases that specifically phosphorylate tyrosine residues. nRTKs are involved in signalling cascades and possess targeting domains such as SH2 domains. (Adapted from FBrf0132098).

Pathway (FlyBase)

NEGATIVE REGULATORS OF HIPPO SIGNALING PATHWAY -: The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Negative regulators of the pathway promote the nuclear accumulation of yki, enhancing yki-mediated transcriptional regulation and the expression of genes that positively regulate tissue growth (Adapted from FBrf0224870).

Protein Function (UniProtKB)

(UniProt, Q9VZI2)

Summary (Interactive Fly)

an evolutionarily conserved non-receptor tyrosine kinases - anti-apoptotic - interacts with Drk (Grb2 homolog) and Yorkie for pro-survival activity and promotion of tissue growth respectively - cooperates with Dpp to regulate myosin levels during dorsal closure - targets the sorting nexin DSH3PX1 to regulate axonal guidance

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Ack for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VZI2)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.43

Gene model reviewed during 5.45

Gene model reviewed during 5.56

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Ack-RA

FBtr0073211

NM_139602

4393

1073

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Ack-PA

FBpp0073067

118.4

1073

7.24

Q9VZI2

NP_647859

AAF47839

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with yki and ex (PubMed:27462444). Interacts with drk (PubMed:22615583). Likely to be a member of an axonal guidance receptor complex that includes SH3PX1, dock and Dscam (PubMed:11773052). Interacts (via N-terminus) with dock (PubMed:11773052, PubMed:23562806). Interacts with SH3PX1 (via SH3 domain) (PubMed:11773052).

(UniProt, Q9VZI2)

Post Translational Modification

Phosphorylated (PubMed:22615583). Autophosphorylated (PubMed:11773052).

(UniProt, Q9VZI2)

Domain

The SAM, Protein kinase and SH3 domains are required for sperm formation.

The SAM and SH3 domains are required for localization to clathrin-coated vesicles in spermatocytes.

(UniProt, Q9VZI2)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ack using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Ack_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

4.75

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism

(Sem et al., 2002)

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13

embryonic leading edge cell

(Sem et al., 2002)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

organism

Comment: head and body

(Sem et al., 2002)

Additional Descriptive Data

Ack transcripts are widely distributed during embryogenesis, and elevated in cells at the leading edge during dorsal closure.

(Sem et al., 2002)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 9

mitotic domain

(Sem et al., 2002)

embryonic stage 15

embryonic leading edge cell

(Sem et al., 2002)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

(Sem et al., 2002)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cell periphery

inferred from direct assay

(Abdallah et al., 2013)

located_in clathrin-coated vesicle

inferred from direct assay

(Abdallah et al., 2013)

located_in cytoophidium

inferred from direct assay

(Strochlic et al., 2014)

located_in cytoplasm

inferred from direct assay

(Schoenherr et al., 2012)

part_of protein-containing complex

inferred from direct assay

(Schoenherr et al., 2012)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Ack in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Ack

View exonic expression by tissue for Dmel\Ack

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0028484

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38489/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0028484

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0028484

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0028484

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG14992

Flygut - An atlas of the Drosophila adult midgut

FBgn0028484

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Ack

Stages(s) 1-3
Stages(s) 4-6

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 6 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 26 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Ack

Transgenic constructs containing regulatory region of Ack

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

Df(3L)Ack^52a

(Zahedi et al., 2008)

Df(3L)C175

(Sem et al., 2002)

Df(3L)Exel6100

(Ryder, 2004.4.26)

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die before end of P-stage, with Scer\GAL4^71B

Ack^UAS.cSa

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

Ack^GD8506

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Ack^GD8506

partially lethal - majority die | heat sensitive, with Scer\GAL4^hs.PB

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Ack^GD8506

viable, with Scer\GAL4^tin.CΔ4

Ack^GD8506

Sterility

Allele

female fertile

Ack⁸⁶

female semi-sterile, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

male sterile

Ack⁸⁶

Other Phenotypes

Allele

increased cell number | adult stage, with Scer\GAL4^NP7397

Ack^UAS.cSa

some die during embryonic stage | heat sensitive, with Scer\GAL4^hs.PB

some die during pharate adult stage, with Scer\GAL4^71B

Ack^UAS.cSa

some die during pupal stage, with Scer\GAL4^pnr-MD237

Ack^GD8506

visible

Ack⁸⁶

visible, with Scer\GAL4^71B

Ack^KD.UAS

visible, with Scer\GAL4^GMR.PF

visible, with Scer\GAL4^GMR.PU

visible, with Scer\GAL4^pnr-MD237

Ack^GD8506

visible | adult stage, with Scer\GAL4^Bx-MS1096

visible | adult stage, with Scer\GAL4^GMR.PU

Ack^UAS.Tag:V5

Phenotype manifest in

Allele

adult midgut, with Scer\GAL4^NP7397

Ack^UAS.cSa

elongation stage spermatid

Ack⁸⁶

embryo | dorsal closure stage, with Scer\GAL4^ptc-559.1

Ack^UAS.cSa

embryo | dorsal closure stage | heat sensitive, with Scer\GAL4^hs.PB

embryonic/first instar larval cuticle | heat sensitive, with Scer\GAL4^hs.PB

embryonic head | heat sensitive, with Scer\GAL4^hs.PB

Ack^KD.UAS

eye

Ack⁸⁶

eye, with Scer\GAL4^GMR.PF

eye, with Scer\GAL4^GMR.PU

eye | adult stage, with Scer\GAL4^GMR.PU

Ack^UAS.Tag:V5

individualization stage spermatid

Ack⁸⁶

interommatidial bristle, with Scer\GAL4^GMR.PF

interommatidial bristle | increased number, with Scer\GAL4^GMR.PU

mesonotum, with Scer\GAL4^pnr-MD237

Ack^GD8506

ommatidium, with Scer\GAL4^GMR.PF

pigment cell, with Scer\GAL4^GMR.PU

Ack^K156A.UAS

spermatozoon

Ack⁸⁶

sperm individualization complex

Ack⁸⁶

wing, with Scer\GAL4^71B

Ack^KD.UAS

wing blade, with Scer\GAL4^Bx-MS1096

wing vein | ectopic, with Scer\GAL4^71B

Ack^KD.UAS

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (97)

Hsap\TNK2

11 of 14

Yes

Hsap\TNK1

5 of 14

Yes

Hsap\AATK

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\LMTK3

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\WNK2

1 of 14

Hsap\YES1

1 of 14

Hsap\ZAP70

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (35)

Rnor\Tnk2

12 of 14

Yes

Rnor\Tnk1

6 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (35)

Mmus\Tnk2

12 of 14

Yes

Mmus\Tnk1

5 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (54)

Xtro\tnk2

9 of 13

Yes

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\tyro3

1 of 13

Yes

Xtro\uqcrfs1

1 of 13

Xtro\zap70

1 of 13

Danio rerio (Zebrafish) (46)

Drer\tnk2a

12 of 14

Yes

Drer\tnk2b

10 of 14

Yes

Drer\tnk1

7 of 14

Yes

Drer\aatkb

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (58)

Cele\sid-3

6 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (35)

Agam\AgaP_AGAP011776

12 of 12

Yes

3 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (24)

Atha\STY8

2 of 13

Yes

Atha\STY17

2 of 13

Yes

Atha\STY46

2 of 13

Yes

Atha\ALE2

1 of 13

Atha\AT1G16270

1 of 13

Atha\AT1G29730

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\CTR1

1 of 13

Atha\HT1

1 of 13

Atha\LYK3

1 of 13

Yes

Atha\PERK12

1 of 13

Atha\VIK

1 of 13

Atha\WAK5

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\CDC15

1 of 13

Yes

Scer\DUN1

1 of 13

Yes

Scer\KIN1

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Ack. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (32)

Ack-like

5 of 13

Abl

4 of 13

Btk

4 of 13

Csk

4 of 13

FER

4 of 13

Src42A

4 of 13

Src64B

4 of 13

Fak

3 of 13

Shark

3 of 13

Alk

2 of 13

btl

2 of 13

Cad96Ca

2 of 13

Egfr

2 of 13

Eph

2 of 13

htl

2 of 13

Nrk

2 of 13

Ret

2 of 13

Ror

2 of 13

CG3277

1 of 13

CG10702

1 of 13

CG17528

1 of 13

dnt

1 of 13

Drl-2

1 of 13

hop

1 of 13

ksr

1 of 13

otk

1 of 13

Pkc53E

1 of 13

robo3

1 of 13

sev

1 of 13

slpr

1 of 13

wnd

1 of 13

Wsck

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TNK2; tyrosine kinase non receptor 2

11 of 14

606994

TNK1; tyrosine kinase non receptor 1

5 of 14

608076

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Dmel gene

Ortholog showing functional complementation

Supporting References

Ack

Mmus\Tnk2

(Abdallah et al., 2013)

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Ack - Gale

anti tag coimmunoprecipitation, peptide massfingerprinting

(Schoenherr et al., 2012)

Ack - Hecw

pull down, Identification by mass spectrometry

(Fajner et al., 2021)

Ack - Hsp23

anti tag coimmunoprecipitation, peptide massfingerprinting

(Schoenherr et al., 2012)

Ack - Hsp26

anti tag coimmunoprecipitation, peptide massfingerprinting

(Schoenherr et al., 2012)

Ack - Hsp83

anti tag coimmunoprecipitation, peptide massfingerprinting

(Schoenherr et al., 2012)

Ack - Magi

anti tag coimmunoprecipitation, peptide massfingerprinting

(Schoenherr et al., 2012)

Ack - Pgam5

anti tag coimmunoprecipitation, peptide massfingerprinting

(Schoenherr et al., 2012)

Ack - SH3PX1

two hybrid, pull down, western blot, enzymatic study, autoradiography, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation, peptide massfingerprinting

(Schoenherr et al., 2012, Worby et al., 2002)

Ack - UQCR-C2

anti tag coimmunoprecipitation, peptide massfingerprinting

(Schoenherr et al., 2012)

Ack - dock

anti bait coimmunoprecipitation, anti tag western blot, pull down, edman degradation, western blot, two hybrid

(Abdallah et al., 2013, Worby et al., 2002)

Ack - drk

anti tag coimmunoprecipitation, peptide massfingerprinting, anti bait coimmunoprecipitation, western blot

(Schoenherr et al., 2012)

Ack - ex

anti tag coimmunoprecipitation, anti tag western blot

(Hu et al., 2016)

Ack - yki

pull down, peptide massfingerprinting, anti tag coimmunoprecipitation, western blot, anti tag western blot

(Hu et al., 2016, Kwon et al., 2013, Schoenherr et al., 2012)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Hu et al., 2016)

suppressible

(Hu et al., 2016)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Sem et al., 2002)

suppressible

(Schoenherr et al., 2012)

hid

suppressible

Ack

(Schoenherr et al., 2012)

hid

suppressible

RasGAP1|Ack

(Schoenherr et al., 2012)

hid

enhanceable

Ack

(Schoenherr et al., 2012)

enhanceable

(Hu et al., 2016)

suppressible

(Gregory et al., 2007)

rpr

suppressible

Ack

(Schoenherr et al., 2012)

tkv

suppressible

Ack

(Zahedi et al., 2008)

enhanceable

(Hu et al., 2016)

suppressible

(Schoenherr et al., 2012)

External Data

Subunit Structure (UniProtKB)

(UniProt, Q9VZI2 )

Linkouts

BioGRID - A database of protein and genetic interactions.

63969

DroID - A comprehensive database of gene and protein interactions.

FBgn0028484

MIST (genetic) - An integrated Molecular Interaction Database

38489

MIST (protein-protein) - An integrated Molecular Interaction Database

38489

Pathways

Signaling Pathways (FlyBase)

NEGATIVE REGULATORS OF HIPPO SIGNALING PATHWAY

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-10

Cytogenetic map

64A6-64A6

Sequence location

3L:4,108,186..4,114,157 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

64A6-64A6

Limits computationally determined from genome sequence between P{PZ}l(3)rG166^rG166 and P{PZ}sinu⁰⁶⁵²⁴

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-10

(FlyBase, 2016)

3-8.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (14)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

27652

y¹ v¹; P{TRiP.JF02729}attP2

22316

y¹ w^67c23; P{EPgy2}Ack^EY09374

35264

y¹ sc^* v¹ sev²¹; P{TRiP.GL00158}attP2

33899

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00839}attP2

Kyoto

200574

y¹ w^67c23; P{GSV1}Ack^GS3234/TM3, Sb¹ Ser¹

NIG-Fly

14992R-1

P{NIG.14992R}1

14992R-3

P{NIG.14992R}3

GL00158

y¹ sc^* v¹ sev²¹; P{TRiP.GL00158}attP2

HMS00839

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00839}attP2

VDRC

v330396

P{VSH330396}attP40

v39857

w¹¹¹⁸; P{GD8506}v39857

v341703

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD00809}attP40/CyO-GFP

More stocks available...

Genomic Clones (16)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (47)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0028484

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0028484

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Hu et al., 2016, Abdallah et al., 2013, Sem et al., 2002, Worby et al., 2002)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Ack is required for the correct subcellular localisation of the dock protein in male germ cells.

(Abdallah et al., 2013)

Ack negatively regulates Egfr in the amnioserosa by targeting it for endocytosis.

(Shen et al., 2013)

When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of S phase cells and an increase in the proportion of G2/M phase cells are seen.

(Bettencourt-Dias et al., 2004)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Identification: as a tyrosine-phosphorylated protein that binds to the SH2 domain of dock.

(Worby et al., 2001, Schmucker et al., 2000)

Relationship to Other Genes

Source for database merge of

Source for merge of: Ack p145

(Worby et al., 2002)

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Ack CG14992

(Clemens et al., 2000)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

ACK

(Zahedi et al., 2002, Morrison et al., 2000)

ACK2

(Morrison et al., 2000)

BcDNA:GH10777

CG14992

(Meadows, 2017.9.14, Hosono et al., 2015, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Perkins et al., 2009, Laviolette et al., 2005, Morrison et al., 2000)

DACK

(Zahedi et al., 2008, Shen et al., 2006, Zahedi et al., 2005, Zahedi et al., 2004, Sem et al., 2002, Harden et al., 2001, Clemens et al., 2000)

DAck

(Thangadurai et al., 2022, Strochlic et al., 2014, Muda et al., 2002, Worby et al., 2002)

dACK

(Zhang et al., 2021)

dAck

(Wang et al., 2025)

p145

(Worby et al., 2002, Worby et al., 2001)

Name Synonyms

Ack

Activated Cdc42 kinase

Secondary FlyBase IDs

FBgn0060299

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 52 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38489

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VZI2

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VZI2

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q8MRP0

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VZI2

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0028484

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38489/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0028484

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0028484

FlyMine - An integrated database for Drosophila genomics

FBgn0028484

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG14992

MARRVEL_MODEL - MARRVEL (model organism gene)

38489

Linkouts

BioGRID - A database of protein and genetic interactions.

63969

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0028484

DroID - A comprehensive database of gene and protein interactions.

FBgn0028484

DRSC - Results frm RNAi screens

FBgn0028484

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Ack_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0028484

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0028484

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG14992

Flygut - An atlas of the Drosophila adult midgut

FBgn0028484

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

38489

MIST (protein-protein) - An integrated Molecular Interaction Database

38489

References (78)

The following external sites may use different assemblies or annotations than FlyBase.

4.75

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